STEPdb: Sub-cellular Topologies of E.coli Polypeptides.

Manual Curation in STEP

Comments

UniProt Name:
Uncharacterized protein YggN
Localization:
Periplasmic
Notes:
Substrate of periplasmic disulfide oxidoreductases DsbA. Identified DsbA substrate PMID: 19953543 PMID: 14726535
References:
PMID: 14726535 PMID: 19953543
Pseudogene/Mobile Element:
-

mRNA Expression

mRNA abundance (Taniguchi 2010)

mRNA molec/cell:
-

Protein Existence

Mass spectrometry experiments

Proteomics:
PMID: 16606699 PMID: 19348461 PMID: 20222674 PMID: 19767571 PMID: 15115803 PMID: 19343722
Proteomics / Inner Membrane fraction:
-

Protein abundance/solubility

Protein abundance (Taniguchi 2010)

Protein molec/cell:
-
PaxDb:
108 ppm

show distribution

EcoCyc

Protein:
EG12705
Gene Locus:
yggN

Structural Annotation

SCOP

Class:
-
Fold:
-
Super Family:
-

Protein Family Domains

Pfam domains

PFAM: P0ADS9

SMART database

SMART: yggN

Protein Complexes

Go to Complexes

Protein Hydrodynamics

Protein Solubility

Niwa et al 2009:
80 % PMID: 19251648

Heat-stability

Heat stable (85oC):
no PMID: 10806384

Disorder

Predict Disorder with IUPred tool:

Predict Now

Bioinformatic tools and predictions

SignalP 4.1

Signal Peptide:
Yes
Cleavage site amino acid:
21
Cleavage Score (Cmax):
0.797

LipoP 1.0

Prediction:
SpI
Cleavage site amino acid:
20
Score:
21.2471
Cleavage Rule:
-

TMHMM v2.0

Number of TMs:
0

Phobius

Signal Peptide:
Yes
Number of TMs:
0

PSORTB (v3.0.2)

Localization:
Unknown
Score:
2

LocTree3

Localization:
periplasm
Score:
74

ClubSub-P

Localization:
-

sosuiGramN

Localization:
P (Periplasm)

PRED-TAT

Localization:
Sec signal peptide predicted. Most likely cleavage site: 1 - 20 [AHA-DY]
Score:
1

TatP

Prediction:
No signal peptide predicted.

Predict Now

Manual Curation in STEP

Comments

UniProt Name:
Protein YgiW
Localization:
Periplasmic
Notes:
SecB substrate. PMID: 9298646
References:
PMID: 16352602 PMID: 9298646
Pseudogene/Mobile Element:
-

mRNA Expression

mRNA abundance (Taniguchi 2010)

mRNA molec/cell:
-

Protein Existence

Mass spectrometry experiments

Proteomics:
PMID: 19348461 PMID: 20222674 PMID: 19767571 PMID: 20803007 PMID: 15115803 PMID: 18425732
Proteomics / Inner Membrane fraction:
-

Protein abundance/solubility

Protein abundance (Taniguchi 2010)

Protein molec/cell:
-
PaxDb:
556 ppm

show distribution

EcoCyc

Protein:
EG13025
Gene Locus:
ygiW

Structural Annotation

SCOP

Class:
All beta proteins
SCOP: 48724
Fold:
OB-fold
SCOP: 50198
Super Family:
Hypothetical protein YgiW
SCOP: 101756

Protein Family Domains

Pfam domains

PFAM: P0ADU5

SMART database

SMART: ygiW

Protein Complexes

Go to Complexes

Protein Hydrodynamics

Protein Solubility

Niwa et al 2009:
28 % PMID: 19251648

Heat-stability

Heat stable (85oC):
no PMID: 10806384

Disorder

Predict Disorder with IUPred tool:

Predict Now

Bioinformatic tools and predictions

SignalP 4.1

Signal Peptide:
Yes
Cleavage site amino acid:
21
Cleavage Score (Cmax):
0.877

LipoP 1.0

Prediction:
SpI
Cleavage site amino acid:
20
Score:
26.1756
Cleavage Rule:
-

TMHMM v2.0

Number of TMs:
0

Phobius

Signal Peptide:
Yes
Number of TMs:
0

PSORTB (v3.0.2)

Localization:
Unknown
Score:
2

LocTree3

Localization:
periplasm
Score:
63

ClubSub-P

Localization:
-

sosuiGramN

Localization:
unknown

PRED-TAT

Localization:
Sec signal peptide predicted. Most likely cleavage site: 1 - 20 [VMA-AE]
Score:
0.999

TatP

Prediction:
No signal peptide predicted.

Predict Now

Manual Curation in STEP

Comments

UniProt Name:
Lipopolysaccharide export system protein LptA
Localization:
Periplasmic
Notes:
-
References:
PMID: 17056748 PMID: 18480051 PMID: 9298646
Pseudogene/Mobile Element:
-

mRNA Expression

mRNA abundance (Taniguchi 2010)

mRNA molec/cell:
-

Protein Existence

Mass spectrometry experiments

Proteomics:
PMID: 20222674 PMID: 19767571 PMID: 20803007 PMID: 18425732
Proteomics / Inner Membrane fraction:
-

Protein abundance/solubility

Protein abundance (Taniguchi 2010)

Protein molec/cell:
-
PaxDb:
87.8 ppm

show distribution

EcoCyc

Protein:
EG12618
Gene Locus:
lptA

Structural Annotation

SCOP

Class:
-
Fold:
-
Super Family:
-

Protein Family Domains

Pfam domains

PFAM: P0ADV1

SMART database

SMART: lptA

Protein Complexes

Go to Complexes

Protein Hydrodynamics

Protein Solubility

Niwa et al 2009:
23 % PMID: 19251648

Heat-stability

Heat stable (85oC):
no PMID: 10806384

Disorder

Predict Disorder with IUPred tool:

Predict Now

Bioinformatic tools and predictions

SignalP 4.1

Signal Peptide:
Yes
Cleavage site amino acid:
28
Cleavage Score (Cmax):
0.838

LipoP 1.0

Prediction:
SpI
Cleavage site amino acid:
27
Score:
24.4302
Cleavage Rule:
-

TMHMM v2.0

Number of TMs:
0

Phobius

Signal Peptide:
Yes
Number of TMs:
0

PSORTB (v3.0.2)

Localization:
Unknown
Score:
2

LocTree3

Localization:
periplasm
Score:
100

ClubSub-P

Localization:
Periplasmic

sosuiGramN

Localization:
unknown

PRED-TAT

Localization:
Sec signal peptide predicted. Most likely cleavage site: 1 - 27 [AFA-VT]
Score:
1

TatP

Prediction:
No signal peptide predicted.

Predict Now

Manual Curation in STEP

Comments

UniProt Name:
Intermembrane phospholipid transport system binding protein MlaC
Localization:
Periplasmic
Notes:
-
References:
PMID: 15911532
Pseudogene/Mobile Element:
-

mRNA Expression

mRNA abundance (Taniguchi 2010)

mRNA molec/cell:
1

Protein Existence

Mass spectrometry experiments

Proteomics:
PMID: 16606699 PMID: 20222674 PMID: 16967446 PMID: 19767571 PMID: 20803007 PMID: 18425732
Proteomics / Inner Membrane fraction:
-

Protein abundance/solubility

Protein abundance (Taniguchi 2010)

Protein molec/cell:
2
PaxDb:
482 ppm

show distribution

EcoCyc

Protein:
EG12798
Gene Locus:
mlaC

Structural Annotation

SCOP

Class:
-
Fold:
-
Super Family:
-

Protein Family Domains

Pfam domains

PFAM: P0ADV7

SMART database

SMART: mlaC

Protein Complexes

Go to Complexes

Protein Hydrodynamics

Protein Solubility

Niwa et al 2009:
61 % PMID: 19251648

Heat-stability

Heat stable (85oC):
no PMID: 10806384

Disorder

Predict Disorder with IUPred tool:

Predict Now

Bioinformatic tools and predictions

SignalP 4.1

Signal Peptide:
Yes
Cleavage site amino acid:
22
Cleavage Score (Cmax):
0.509

LipoP 1.0

Prediction:
SpI
Cleavage site amino acid:
21
Score:
14.0896
Cleavage Rule:
-

TMHMM v2.0

Number of TMs:
0

Phobius

Signal Peptide:
Yes
Number of TMs:
0

PSORTB (v3.0.2)

Localization:
Unknown
Score:
2

LocTree3

Localization:
periplasm
Score:
63

ClubSub-P

Localization:
Periplasmic

sosuiGramN

Localization:
P (Periplasm)

PRED-TAT

Localization:
Sec signal peptide predicted. Most likely cleavage site: 1 - 21 [ATA-AD]
Score:
0.999

TatP

Prediction:
No signal peptide predicted.

Predict Now

Manual Curation in STEP

Comments

UniProt Name:
Uncharacterized protein YhhA (ORFQ)
Localization:
-
Notes:
-
References:
-
Pseudogene/Mobile Element:
-

mRNA Expression

mRNA abundance (Taniguchi 2010)

mRNA molec/cell:
-

Protein Existence

Mass spectrometry experiments

Proteomics:
PMID: 16606699 PMID: 12488465 PMID: 20222674 PMID: 19767571 PMID: 20803007
Proteomics / Inner Membrane fraction:
-

Protein abundance/solubility

Protein abundance (Taniguchi 2010)

Protein molec/cell:
-
PaxDb:
199 ppm

show distribution

EcoCyc

Protein:
EG11184
Gene Locus:
yhhA

Structural Annotation

SCOP

Class:
-
Fold:
-
Super Family:
-

Protein Family Domains

Pfam domains

PFAM: P0ADX7

SMART database

SMART: yhhA

Protein Complexes

Go to Complexes

Protein Hydrodynamics

Protein Solubility

Niwa et al 2009:
107 % PMID: 19251648

Heat-stability

Heat stable (85oC):
no PMID: 10806384

Disorder

Predict Disorder with IUPred tool:

Predict Now

Bioinformatic tools and predictions

SignalP 4.1

Signal Peptide:
Yes
Cleavage site amino acid:
18
Cleavage Score (Cmax):
0.377

LipoP 1.0

Prediction:
SpI
Cleavage site amino acid:
17
Score:
14.5283
Cleavage Rule:
-

TMHMM v2.0

Number of TMs:
0

Phobius

Signal Peptide:
Yes
Number of TMs:
0

PSORTB (v3.0.2)

Localization:
Unknown
Score:
2

LocTree3

Localization:
secreted
Score:
61

ClubSub-P

Localization:
-

sosuiGramN

Localization:
OM (Outer Membrane)

PRED-TAT

Localization:
Sec signal peptide predicted. Most likely cleavage site: 1 - 17 [GFA-QP]
Score:
0.998

TatP

Prediction:
No signal peptide predicted.

Predict Now

Manual Curation in STEP

Comments

UniProt Name:
Multidrug efflux pump subunit AcrA (AcrAB-TolC multidrug efflux pump subunit AcrA) (Acridine resistance protein A)
Localization:
Inner Membrane Lipoprotein
Notes:
Proven signal peptidase II substrate. PMID: 10894736
References:
PMID: 10894736 PMID: 15174130 PMID: 15228545 PMID: 16079137
Pseudogene/Mobile Element:
-

mRNA Expression

mRNA abundance (Taniguchi 2010)

mRNA molec/cell:
-

Protein Existence

Mass spectrometry experiments

Proteomics:
PMID: 12624733 PMID: 12488465 PMID: 17137328 PMID: 19348461 PMID: 20222674 PMID: 16967446 PMID: 19767571 PMID: 16079137 PMID: 15115803 PMID: 20932056 PMID: 18796603
Proteomics / Inner Membrane fraction:
PMID: 16079137 PMID: 17309111

Protein abundance/solubility

Protein abundance (Taniguchi 2010)

Protein molec/cell:
-
PaxDb:
256 ppm

show distribution

EcoCyc

Protein:
EG11703
Gene Locus:
acrA

Structural Annotation

SCOP

Class:
Membrane and cell surface proteins and peptides
SCOP: 56835
Fold:
HlyD-like secretion proteins
SCOP: 111368
Super Family:
HlyD-like secretion proteins
SCOP: 111369

Protein Family Domains

Pfam domains

PFAM: P0AE06

SMART database

SMART: acrA

Protein Complexes

Go to Complexes

Protein Hydrodynamics

Protein Solubility

Niwa et al 2009:
64 % PMID: 19251648

Heat-stability

Heat stable (85oC):
no PMID: 10806384

Disorder

Predict Disorder with IUPred tool:

Predict Now

Bioinformatic tools and predictions

SignalP 4.1

Signal Peptide:
Yes
Cleavage site amino acid:
22
Cleavage Score (Cmax):
0.194

LipoP 1.0

Prediction:
SpII
Cleavage site amino acid:
24
Score:
19.636
Cleavage Rule:
Pos+2=D

TMHMM v2.0

Number of TMs:
0

Phobius

Signal Peptide:
Yes
Number of TMs:
0

PSORTB (v3.0.2)

Localization:
CytoplasmicMembrane
Score:
10

LocTree3

Localization:
inner membrane
Score:
16

ClubSub-P

Localization:
Inner/Outer-membrane lipoprotein

sosuiGramN

Localization:
OM (Outer Membrane)

PRED-TAT

Localization:
Sec signal peptide predicted. Most likely cleavage site: 1 - 21 [SLA-LT]
Score:
0.997

TatP

Prediction:
No signal peptide predicted.

Predict Now

Manual Curation in STEP

Comments

UniProt Name:
Class B acid phosphatase (CBAP) (EC 3.1.3.2)
Localization:
Periplasmic
Notes:
-
References:
PMID: 9011040 PMID: 9298646
Pseudogene/Mobile Element:
-

mRNA Expression

mRNA abundance (Taniguchi 2010)

mRNA molec/cell:
-

Protein Existence

Mass spectrometry experiments

Proteomics:
PMID: 16606699 PMID: 12488465 PMID: 20222674 PMID: 19767571 PMID: 15115803
Proteomics / Inner Membrane fraction:
-

Protein abundance/solubility

Protein abundance (Taniguchi 2010)

Protein molec/cell:
-
PaxDb:
58.6 ppm

show distribution

EcoCyc

Protein:
EG11934
Gene Locus:
aphA

Structural Annotation

SCOP

Class:
Alpha and beta proteins (a/b)
SCOP: 51349
Fold:
HAD-like
SCOP: 56783
Super Family:
HAD-like
SCOP: 56784

Protein Family Domains

Pfam domains

PFAM: P0AE22

SMART database

SMART: aphA

Protein Complexes

Go to Complexes

Protein Hydrodynamics

Protein Solubility

Niwa et al 2009:
% PMID: 19251648

Heat-stability

Heat stable (85oC):
no PMID: 10806384

Disorder

Predict Disorder with IUPred tool:

Predict Now

Bioinformatic tools and predictions

SignalP 4.1

Signal Peptide:
Yes
Cleavage site amino acid:
26
Cleavage Score (Cmax):
0.643

LipoP 1.0

Prediction:
SpI
Cleavage site amino acid:
25
Score:
14.5914
Cleavage Rule:
-

TMHMM v2.0

Number of TMs:
0

Phobius

Signal Peptide:
Yes
Number of TMs:
0

PSORTB (v3.0.2)

Localization:
Unknown
Score:
2

LocTree3

Localization:
periplasm
Score:
100

ClubSub-P

Localization:
Periplasmic

sosuiGramN

Localization:
P (Periplasm)

PRED-TAT

Localization:
Sec signal peptide predicted. Most likely cleavage site: 1 - 25 [ALA-SS]
Score:
0.957

TatP

Prediction:
No signal peptide predicted.

Predict Now

Manual Curation in STEP

Comments

UniProt Name:
Periplasmic protein CpxP (ORF_o167) (Periplasmic accessory protein CpxP)
Localization:
Peripheral inner membrane protein facing the periplasm
Notes:
-
References:
PMID: 10972835 PMID: 17904518 PMID: 21317318
Pseudogene/Mobile Element:
pseudogene (Ochman & Davalos, 2006)

mRNA Expression

mRNA abundance (Taniguchi 2010)

mRNA molec/cell:
-

Protein Existence

Mass spectrometry experiments

Proteomics:
PMID: 16606699 PMID: 12488465 PMID: 20222674 PMID: 20803007
Proteomics / Inner Membrane fraction:
-

Protein abundance/solubility

Protein abundance (Taniguchi 2010)

Protein molec/cell:
-
PaxDb:
0 ppm

EcoCyc

Protein:
EG11872
Gene Locus:
cpxP

Structural Annotation

SCOP

Class:
-
Fold:
-
Super Family:
-

Protein Family Domains

Pfam domains

PFAM: P0AE85

SMART database

SMART: cpxP

Protein Complexes

Go to Complexes

Protein Hydrodynamics

Protein Solubility

Niwa et al 2009:
104 % PMID: 19251648

Heat-stability

Heat stable (85oC):
no PMID: 10806384

Disorder

Predict Disorder with IUPred tool:

Predict Now

Bioinformatic tools and predictions

SignalP 4.1

Signal Peptide:
Yes
Cleavage site amino acid:
22
Cleavage Score (Cmax):
0.806

LipoP 1.0

Prediction:
SpI
Cleavage site amino acid:
21
Score:
16.0374
Cleavage Rule:
-

TMHMM v2.0

Number of TMs:
0

Phobius

Signal Peptide:
Yes
Number of TMs:
0

PSORTB (v3.0.2)

Localization:
Periplasmic
Score:
10

LocTree3

Localization:
periplasm
Score:
100

ClubSub-P

Localization:
Periplasmic

sosuiGramN

Localization:
P (Periplasm)

PRED-TAT

Localization:
Sec signal peptide predicted. Most likely cleavage site: 1 - 21 [SHA-AE]
Score:
1

TatP

Prediction:
No signal peptide predicted.

Predict Now

Manual Curation in STEP

Comments

UniProt Name:
Protein CreA (Catabolite regulation protein A)
Localization:
Periplasmic
Notes:
Identified DsbA substrate PMID: 19953543 Disulfide bond-containing protein PMID: 25664544
References:
PMID: 19953543 PMID: 25664544
Pseudogene/Mobile Element:
-

mRNA Expression

mRNA abundance (Taniguchi 2010)

mRNA molec/cell:
-

Protein Existence

Mass spectrometry experiments

Proteomics:
PMID: 16606699 PMID: 20222674 PMID: 19767571 PMID: 20803007 PMID: 15115803
Proteomics / Inner Membrane fraction:
-

Protein abundance/solubility

Protein abundance (Taniguchi 2010)

Protein molec/cell:
-
PaxDb:
49.7 ppm

show distribution

EcoCyc

Protein:
EG11217
Gene Locus:
creA

Structural Annotation

SCOP

Class:
-
Fold:
-
Super Family:
-

Protein Family Domains

Pfam domains

PFAM: P0AE91

SMART database

SMART: creA

Protein Complexes

Go to Complexes

Protein Hydrodynamics

Protein Solubility

Niwa et al 2009:
% PMID: 19251648

Heat-stability

Heat stable (85oC):
no PMID: 10806384

Disorder

Predict Disorder with IUPred tool:

Predict Now

Bioinformatic tools and predictions

SignalP 4.1

Signal Peptide:
Yes
Cleavage site amino acid:
22
Cleavage Score (Cmax):
0.8

LipoP 1.0

Prediction:
SpI
Cleavage site amino acid:
21
Score:
14.9398
Cleavage Rule:
-

TMHMM v2.0

Number of TMs:
0

Phobius

Signal Peptide:
Yes
Number of TMs:
0

PSORTB (v3.0.2)

Localization:
Unknown
Score:
2

LocTree3

Localization:
periplasm
Score:
51

ClubSub-P

Localization:
Periplasmic

sosuiGramN

Localization:
unknown

PRED-TAT

Localization:
Sec signal peptide predicted. Most likely cleavage site: 1 - 21 [AHA-EE]
Score:
1

TatP

Prediction:
No signal peptide predicted.

Predict Now

Manual Curation in STEP

Comments

UniProt Name:
Curli production assembly/transport component CsgE
Localization:
Periplasmic
Notes:
Potential substrate of periplasmic peptidyl prolyl cis???trans isomerase SurA; sequence contains known binding motif of SurA [RYEF]X[RYEF][P]. Curli secretion specificity factor. CsgE provides substrate specificity to the curli secretion pore CsgG, and acts directly on the secretion substrate CsgA to prevent premature subunit assembly. PMID: 9928475
References:
PMID: 16704339 PMID: 18498364 PMID: 19131513 PMID: 21645131 PMID: 9928475
Pseudogene/Mobile Element:
-

mRNA Expression

mRNA abundance (Taniguchi 2010)

mRNA molec/cell:
-

Protein Existence

Mass spectrometry experiments

Proteomics:
-
Proteomics / Inner Membrane fraction:
-

Protein abundance/solubility

Protein abundance (Taniguchi 2010)

Protein molec/cell:
-
PaxDb:
0 ppm

EcoCyc

Protein:
EG13411
Gene Locus:
csgE

Structural Annotation

SCOP

Class:
-
Fold:
-
Super Family:
-

Protein Family Domains

Pfam domains

PFAM: P0AE95

SMART database

SMART: csgE

Protein Complexes

Go to Complexes

Protein Hydrodynamics

Protein Solubility

Niwa et al 2009:
15 % PMID: 19251648

Heat-stability

Heat stable (85oC):
no PMID: 10806384

Disorder

Predict Disorder with IUPred tool:

Predict Now

Bioinformatic tools and predictions

SignalP 4.1

Signal Peptide:
Yes
Cleavage site amino acid:
23
Cleavage Score (Cmax):
0.789

LipoP 1.0

Prediction:
SpI
Cleavage site amino acid:
22
Score:
3.89784
Cleavage Rule:
-

TMHMM v2.0

Number of TMs:
0

Phobius

Signal Peptide:
Yes
Number of TMs:
0

PSORTB (v3.0.2)

Localization:
Unknown
Score:
2

LocTree3

Localization:
secreted
Score:
41

ClubSub-P

Localization:
Periplasmic

sosuiGramN

Localization:
P (Periplasm)

PRED-TAT

Localization:
Sec signal peptide predicted. Most likely cleavage site: 1 - 22 [LHA-VE]
Score:
1

TatP

Prediction:
No signal peptide predicted.

Predict Now

Manual Curation in STEP

Comments

UniProt Name:
Curli production assembly/transport component CsgF
Localization:
Peripheral outer membrane protein facing the extra-cellular space
Notes:
Curli component. CsgF is secreted to the cell surface, where it mediates the cell-association and protease-resistance of the CsgB nucleator, suggesting that CsgF is required for specific localization and/or chaperoning of CsgB for full nucleator activity. Curli are a functional amyloid whose in vivo polymerization requires a dedicated nucleator protein, CsgB, and an assembly protein, CsgF. Without CsgF, curli subunits are released from the cell into the media and are inefficiently polymerized, resulting in fewer and mislocalized curli fibers. PMID: 19131513 PMID: 9928475
References:
PMID: 16522795 PMID: 16704339 PMID: 19131513 PMID: 21645131 PMID: 9928475
Pseudogene/Mobile Element:
-

mRNA Expression

mRNA abundance (Taniguchi 2010)

mRNA molec/cell:
-

Protein Existence

Mass spectrometry experiments

Proteomics:
-
Proteomics / Inner Membrane fraction:
-

Protein abundance/solubility

Protein abundance (Taniguchi 2010)

Protein molec/cell:
-
PaxDb:
0 ppm

EcoCyc

Protein:
EG13412
Gene Locus:
csgF

Structural Annotation

SCOP

Class:
-
Fold:
-
Super Family:
-

Protein Family Domains

Pfam domains

PFAM: P0AE98

SMART database

SMART: csgF

Protein Complexes

Go to Complexes

Protein Hydrodynamics

Protein Solubility

Niwa et al 2009:
30 % PMID: 19251648

Heat-stability

Heat stable (85oC):
no PMID: 10806384

Disorder

Predict Disorder with IUPred tool:

Predict Now

Bioinformatic tools and predictions

SignalP 4.1

Signal Peptide:
Yes
Cleavage site amino acid:
20
Cleavage Score (Cmax):
0.853

LipoP 1.0

Prediction:
SpI
Cleavage site amino acid:
19
Score:
13.9164
Cleavage Rule:
-

TMHMM v2.0

Number of TMs:
1

Phobius

Signal Peptide:
Yes
Number of TMs:
0

PSORTB (v3.0.2)

Localization:
Extracellular
Score:
9

LocTree3

Localization:
secreted
Score:
34

ClubSub-P

Localization:
Extracellular AND signal peptide

sosuiGramN

Localization:
EC (Extracellular)

PRED-TAT

Localization:
Sec signal peptide predicted. Most likely cleavage site: 1 - 19 [SWA-GT]
Score:
0.853

TatP

Prediction:
No signal peptide predicted.

Predict Now

Manual Curation in STEP

Comments

UniProt Name:
Curli production assembly/transport component CsgG
Localization:
Outer Membrane Lipoprotein, Peripheral outer membrane protein facing the extra-cellular space
Notes:
Curli component. Proven signal peptidase II substrate. CsgG is an outer membrane protein required for the secretion of the two curli structural subunits CsgA and CsgB. CsgG is a lipoprotein that spans the outermembrane and assembles into an oligomeric annular-shaped structure with an inner diameter of approximately 2 nm (Margery L. Evans and Matthew R. Chapman in press). Overexpression of CsgG results in increased sensitivity to the small antibiotic erythromycin (740Da) but not the larger vancomycin (1440Da) suggesting that CsgG has a pore-like activity (Robinson et al 2006). PMID: 15174130
References:
PMID: 15174130 PMID: 16420357 PMID: 16704339 PMID: 19131513 PMID: 21645131
Pseudogene/Mobile Element:
-

mRNA Expression

mRNA abundance (Taniguchi 2010)

mRNA molec/cell:
1

Protein Existence

Mass spectrometry experiments

Proteomics:
PMID: 17595003
Proteomics / Inner Membrane fraction:
-

Protein abundance/solubility

Protein abundance (Taniguchi 2010)

Protein molec/cell:
2
PaxDb:
22.4 ppm

show distribution

EcoCyc

Protein:
EG13413
Gene Locus:
csgG

Structural Annotation

SCOP

Class:
-
Fold:
-
Super Family:
-

Protein Family Domains

Pfam domains

PFAM: P0AEA2

SMART database

SMART: csgG

Protein Complexes

Go to Complexes

Protein Hydrodynamics

Protein Solubility

Niwa et al 2009:
44 % PMID: 19251648

Heat-stability

Heat stable (85oC):
no PMID: 10806384

Disorder

Predict Disorder with IUPred tool:

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Bioinformatic tools and predictions

SignalP 4.1

Signal Peptide:
Yes
Cleavage site amino acid:
20
Cleavage Score (Cmax):
0.544

LipoP 1.0

Prediction:
SpII
Cleavage site amino acid:
15
Score:
12.9163
Cleavage Rule:
Pos+2=L

TMHMM v2.0

Number of TMs:
0

Phobius

Signal Peptide:
Yes
Number of TMs:
0

PSORTB (v3.0.2)

Localization:
CytoplasmicMembrane
Score:
9

LocTree3

Localization:
secreted
Score:
33

ClubSub-P

Localization:
Inner/Outer-membrane lipoprotein

sosuiGramN

Localization:
OM (Outer Membrane)

PRED-TAT

Localization:
Sec signal peptide predicted. Most likely cleavage site: 1 - 25 [EAA-RP]
Score:
0.993

TatP

Prediction:
No signal peptide predicted.

Predict Now

Manual Curation in STEP

Comments

UniProt Name:
D-alanyl-D-alanine carboxypeptidase DacA (DD-carboxypeptidase) (DD-peptidase) (EC 3.4.16.4) (Beta-lactamase) (EC 3.5.2.6) (Penicillin-binding protein 5) (PBP-5)
Localization:
Peripheral inner membrane protein facing the periplasm
Notes:
Anchored to inner membrane by C-terminal tailPMID: 2194801. Using natural isotope abundance NMR, the structure of the PBP 5 anchor peptide within a micelle was determinedPMID: 20192190. The structure conforms to a helix-bend-helix-turn-helix motif and reveals that the anchor enters the membrane so as to form an amphiphilic structure within the interface of the hydrophilic/hydrophobic boundary regions near the lipid head groups. PBP-5 deletion mutants exhibit irregular distribution of MreB at the deformed zones PMID: 20545860. PMID: 3279397 PMID: 8424800 DacA(PBP-5) anchors to the periplasmic face of the inner membrane via C-terminal amphiphilic alpha-helix. The Alpha-helical conformation in the C-terminal anchoring domain is observed in aqueous solution or in the presence of phospholipid vesicles. A pH dependent loss of alpha-helical conformation in the peptide corresponding to the PBP5 C-terminus was found to correlate with the susceptibility of PBP5 to membrane extraction. PMID: 9371419
References:
PMID: 20192190 PMID: 20545860 PMID: 2194801 PMID: 24057063 PMID: 3279397 PMID: 8424800 PMID: 9371419
Pseudogene/Mobile Element:
-

mRNA Expression

mRNA abundance (Taniguchi 2010)

mRNA molec/cell:
3

Protein Existence

Mass spectrometry experiments

Proteomics:
PMID: 16606699 PMID: 12624733 PMID: 12488465 PMID: 19348461 PMID: 20222674 PMID: 16967446 PMID: 19767571 PMID: 20521846 PMID: 15115803 PMID: 20932056 PMID: 18796603
Proteomics / Inner Membrane fraction:
PMID: 17309111

Protein abundance/solubility

Protein abundance (Taniguchi 2010)

Protein molec/cell:
5
PaxDb:
195 ppm

show distribution

EcoCyc

Protein:
EG10201
Gene Locus:
dacA

Structural Annotation

SCOP

Class:
Multi-domain proteins (alpha and beta)
SCOP: 56572
Fold:
beta-lactamase/transpeptidase-like
SCOP: 56600
Penicillin-binding protein associated domain
SCOP: 69188
Super Family:
beta-lactamase/transpeptidase-like
SCOP: 56601
Penicillin-binding protein associated domain
SCOP: 69189

Protein Family Domains

Pfam domains

PFAM: P0AEB2

SMART database

SMART: dacA

Protein Complexes

Go to Complexes

Protein Hydrodynamics

Protein Solubility

Niwa et al 2009:
8 % PMID: 19251648

Heat-stability

Heat stable (85oC):
no PMID: 10806384

Disorder

Predict Disorder with IUPred tool:

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Bioinformatic tools and predictions

SignalP 4.1

Signal Peptide:
Yes
Cleavage site amino acid:
30
Cleavage Score (Cmax):
0.737

LipoP 1.0

Prediction:
SpI
Cleavage site amino acid:
29
Score:
17.6151
Cleavage Rule:
-

TMHMM v2.0

Number of TMs:
0

Phobius

Signal Peptide:
Yes
Number of TMs:
0

PSORTB (v3.0.2)

Localization:
CytoplasmicMembrane
Score:
9

LocTree3

Localization:
secreted
Score:
17

ClubSub-P

Localization:
Periplasmic

sosuiGramN

Localization:
EC (Extracellular)

PRED-TAT

Localization:
Sec signal peptide predicted. Most likely cleavage site: 1 - 29 [AHA-DD]
Score:
1

TatP

Prediction:
No signal peptide predicted.

Predict Now

Manual Curation in STEP

Comments

UniProt Name:
Protein DcrB
Localization:
Inner Membrane Lipoprotein
Notes:
-
References:
PMID: 12558182 PMID: 15911532
Pseudogene/Mobile Element:
-

mRNA Expression

mRNA abundance (Taniguchi 2010)

mRNA molec/cell:
3

Protein Existence

Mass spectrometry experiments

Proteomics:
PMID: 16606699 PMID: 12488465 PMID: 19348461 PMID: 20222674 PMID: 16967446 PMID: 16858726 PMID: 19767571 PMID: 20803007 PMID: 15115803 PMID: 20932056
Proteomics / Inner Membrane fraction:
PMID: 17446557 PMID: 17309111

Protein abundance/solubility

Protein abundance (Taniguchi 2010)

Protein molec/cell:
5
PaxDb:
262 ppm

show distribution

EcoCyc

Protein:
EG12218
Gene Locus:
dcrB

Structural Annotation

SCOP

Class:
-
Fold:
-
Super Family:
-

Protein Family Domains

Pfam domains

PFAM: P0AEE1

SMART database

SMART: dcrB

Protein Complexes

Go to Complexes

Protein Hydrodynamics

Protein Solubility

Niwa et al 2009:
100 % PMID: 19251648

Heat-stability

Heat stable (85oC):
no PMID: 10806384

Disorder

Predict Disorder with IUPred tool:

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Bioinformatic tools and predictions

SignalP 4.1

Signal Peptide:
No
Cleavage site amino acid:
30
Cleavage Score (Cmax):
0.169

LipoP 1.0

Prediction:
SpII
Cleavage site amino acid:
19
Score:
21.1
Cleavage Rule:
Pos+2=D

TMHMM v2.0

Number of TMs:
0

Phobius

Signal Peptide:
Yes
Number of TMs:
0

PSORTB (v3.0.2)

Localization:
Periplasmic
Score:
10

LocTree3

Localization:
periplasm
Score:
100

ClubSub-P

Localization:
Inner/Outer-membrane lipoprotein

sosuiGramN

Localization:
OM (Outer Membrane)

PRED-TAT

Localization:
Sec signal peptide predicted. Most likely cleavage site: 1 - 29 [ATA-QG]
Score:
0.982

TatP

Prediction:
No signal peptide predicted.

Predict Now

Manual Curation in STEP

Comments

UniProt Name:
Serine endoprotease DegS (EC 3.4.21.107) (Site-1 protease DegS) (S1P protease DegS) (Site-1-type intramembrane protease)
Localization:
Periplasmic
Notes:
Proteolysis of transmembrane and cytoplasmic portions of RseA by DegS frees the sigma(E) transcription factor. RseB protein, a known negative regulator, inhibits proteolysis by DegS in vitro by binding tightly to the periplasmic domain of RseA. PMID: 17360428
References:
PMID: 11442831 PMID: 17360428
Pseudogene/Mobile Element:
-

mRNA Expression

mRNA abundance (Taniguchi 2010)

mRNA molec/cell:
-

Protein Existence

Mass spectrometry experiments

Proteomics:
PMID: 19348461 PMID: 20222674 PMID: 19767571
Proteomics / Inner Membrane fraction:
PMID: 17309111

Protein abundance/solubility

Protein abundance (Taniguchi 2010)

Protein molec/cell:
-
PaxDb:
6.32 ppm

show distribution

EcoCyc

Protein:
EG11652
Gene Locus:
degS

Structural Annotation

SCOP

Class:
All beta proteins
SCOP: 48724
Fold:
PDZ domain-like
SCOP: 50155
Trypsin-like serine proteases
SCOP: 50493
Super Family:
PDZ domain-like
SCOP: 50156
Trypsin-like serine proteases
SCOP: 50494

Protein Family Domains

Pfam domains

PFAM: P0AEE3

SMART database

SMART: degS

Protein Complexes

Go to Complexes

Protein Hydrodynamics

Protein Solubility

Niwa et al 2009:
75 % PMID: 19251648

Heat-stability

Heat stable (85oC):
no PMID: 10806384

Disorder

Predict Disorder with IUPred tool:

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Bioinformatic tools and predictions

SignalP 4.1

Signal Peptide:
No
Cleavage site amino acid:
28
Cleavage Score (Cmax):
0.167

LipoP 1.0

Prediction:
SpI
Cleavage site amino acid:
28
Score:
4.49468
Cleavage Rule:
-

TMHMM v2.0

Number of TMs:
1

Phobius

Signal Peptide:
Yes
Number of TMs:
0

PSORTB (v3.0.2)

Localization:
Periplasmic
Score:
9

LocTree3

Localization:
periplasm
Score:
31

ClubSub-P

Localization:
Uncertain

sosuiGramN

Localization:
OM (Outer Membrane)

PRED-TAT

Localization:
Sec signal peptide predicted. Most likely cleavage site: 1 - 28 [LRS-LN]
Score:
0.918

TatP

Prediction:
No signal peptide predicted.

Predict Now

Manual Curation in STEP

Comments

UniProt Name:
D-galactose-binding periplasmic protein (GBP) (D-galactose/ D-glucose-binding protein) (GGBP)
Localization:
Periplasmic
Notes:
Potential Skp substrate PMID: 19953543 PMID: 7012152 PMID: 9298646
References:
PMID: 19953543 PMID: 7012152 PMID: 9298646
Pseudogene/Mobile Element:
-

mRNA Expression

mRNA abundance (Taniguchi 2010)

mRNA molec/cell:
-

Protein Existence

Mass spectrometry experiments

Proteomics:
PMID: 16606699 PMID: 12488465 PMID: 19348461 PMID: 20222674 PMID: 19003857 PMID: 19767571 PMID: 15115803 PMID: 20923772
Proteomics / Inner Membrane fraction:
-

Protein abundance/solubility

Protein abundance (Taniguchi 2010)

Protein molec/cell:
-
PaxDb:
2392 ppm

show distribution

EcoCyc

Protein:
EG10593
Gene Locus:
mglB

Structural Annotation

SCOP

Class:
Alpha and beta proteins (a/b)
SCOP: 51349
Fold:
Periplasmic binding protein-like I
SCOP: 53821
Super Family:
Periplasmic binding protein-like I
SCOP: 53822

Protein Family Domains

Pfam domains

PFAM: P0AEE5

SMART database

SMART: mglB

Protein Complexes

Go to Complexes

Protein Hydrodynamics

Protein Solubility

Niwa et al 2009:
75 % PMID: 19251648

Heat-stability

Heat stable (85oC):
no PMID: 10806384

Disorder

Predict Disorder with IUPred tool:

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Bioinformatic tools and predictions

SignalP 4.1

Signal Peptide:
Yes
Cleavage site amino acid:
24
Cleavage Score (Cmax):
0.861

LipoP 1.0

Prediction:
SpI
Cleavage site amino acid:
23
Score:
23.7963
Cleavage Rule:
-

TMHMM v2.0

Number of TMs:
1

Phobius

Signal Peptide:
Yes
Number of TMs:
0

PSORTB (v3.0.2)

Localization:
Periplasmic
Score:
10

LocTree3

Localization:
periplasm
Score:
100

ClubSub-P

Localization:
Periplasmic

sosuiGramN

Localization:
P (Periplasm)

PRED-TAT

Localization:
Sec signal peptide predicted. Most likely cleavage site: 1 - 23 [AHA-AD]
Score:
1

TatP

Prediction:
No signal peptide predicted.

Predict Now

Manual Curation in STEP

Comments

UniProt Name:
Thiol:disulfide interchange protein DsbA
Localization:
Periplasmic
Notes:
Periplasmic disulfide isomerase DsbC cooperates with SurA and the thiol oxidase DsbA in the folding of the essential ??-barrel protein LptD. DsbA is co-translationally translocated through Sec translocon. PMID: 9298646 DsbA, is a substrate for the SRP pathway. PMID: 15838024 DsbA, is a substrate for the SRP pathway. PMID: 13129941 Disulfide bridge (49 52) PMID: 9300489 Disulfide bond-containing protein PMID: 25664544
References:
PMID: 13129941 PMID: 15838024 PMID: 18406599 PMID: 19525348 PMID: 20615876 PMID: 25664544 PMID: 9298646 PMID: 9300489
Pseudogene/Mobile Element:
-

mRNA Expression

mRNA abundance (Taniguchi 2010)

mRNA molec/cell:
3

Protein Existence

Mass spectrometry experiments

Proteomics:
PMID: 16606699 PMID: 12624733 PMID: 12488465 PMID: 20222674 PMID: 19767571 PMID: 20803007 PMID: 18727129 PMID: 15115803 PMID: 18425732
Proteomics / Inner Membrane fraction:
-

Protein abundance/solubility

Protein abundance (Taniguchi 2010)

Protein molec/cell:
8
PaxDb:
314 ppm

show distribution

EcoCyc

Protein:
EG11297
Gene Locus:
dsbA

Structural Annotation

SCOP

Class:
Alpha and beta proteins (a/b)
SCOP: 51349
Fold:
Thioredoxin fold
SCOP: 52832
Super Family:
Thioredoxin-like
SCOP: 52833

Protein Family Domains

Pfam domains

PFAM: P0AEG4

SMART database

SMART: dsbA

Protein Complexes

Go to Complexes

Protein Hydrodynamics

Protein Solubility

Niwa et al 2009:
71 % PMID: 19251648

Heat-stability

Heat stable (85oC):
no PMID: 10806384

Disorder

Predict Disorder with IUPred tool:

Predict Now

Bioinformatic tools and predictions

SignalP 4.1

Signal Peptide:
Yes
Cleavage site amino acid:
20
Cleavage Score (Cmax):
0.75

LipoP 1.0

Prediction:
SpI
Cleavage site amino acid:
19
Score:
16.7987
Cleavage Rule:
-

TMHMM v2.0

Number of TMs:
0

Phobius

Signal Peptide:
Yes
Number of TMs:
0

PSORTB (v3.0.2)

Localization:
Periplasmic
Score:
10

LocTree3

Localization:
periplasm
Score:
100

ClubSub-P

Localization:
Periplasmic

sosuiGramN

Localization:
P (Periplasm)

PRED-TAT

Localization:
Sec signal peptide predicted. Most likely cleavage site: 1 - 19 [ASA-AQ]
Score:
0.996

TatP

Prediction:
No signal peptide predicted.

Predict Now

Manual Curation in STEP

Comments

UniProt Name:
Thiol:disulfide interchange protein DsbC
Localization:
Periplasmic
Notes:
Periplasmic disulfide isomerase DsbC cooperates with SurA and the thiol oxidase DsbA in the folding of the essential ??-barrel protein LptD. PMID: 9298646 Disulfide bridge (118 121;161 183) PMID: 10700276 Disulfide bond-containing protein PMID: 25664544
References:
PMID: 10700276 PMID: 18406599 PMID: 20615876 PMID: 25664544 PMID: 8168498 PMID: 9298646
Pseudogene/Mobile Element:
-

mRNA Expression

mRNA abundance (Taniguchi 2010)

mRNA molec/cell:
5

Protein Existence

Mass spectrometry experiments

Proteomics:
PMID: 16606699 PMID: 12488465 PMID: 20222674 PMID: 19767571 PMID: 15115803
Proteomics / Inner Membrane fraction:
-

Protein abundance/solubility

Protein abundance (Taniguchi 2010)

Protein molec/cell:
1
PaxDb:
131 ppm

show distribution

EcoCyc

Protein:
EG11070
Gene Locus:
dsbC

Structural Annotation

SCOP

Class:
Alpha and beta proteins (a+b)
SCOP: 53931
Fold:
Thioredoxin fold
SCOP: 52832
Cystatin-like
SCOP: 54402
Super Family:
Thioredoxin-like
SCOP: 52833
DsbC/DsbG N-terminal domain-like
SCOP: 54423

Protein Family Domains

Pfam domains

PFAM: P0AEG6

SMART database

SMART: dsbC

Protein Complexes

Go to Complexes

Protein Hydrodynamics

Protein Solubility

Niwa et al 2009:
82 % PMID: 19251648

Heat-stability

Heat stable (85oC):
no PMID: 10806384

Disorder

Predict Disorder with IUPred tool:

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Bioinformatic tools and predictions

SignalP 4.1

Signal Peptide:
Yes
Cleavage site amino acid:
21
Cleavage Score (Cmax):
0.778

LipoP 1.0

Prediction:
SpI
Cleavage site amino acid:
20
Score:
21.5074
Cleavage Rule:
-

TMHMM v2.0

Number of TMs:
0

Phobius

Signal Peptide:
Yes
Number of TMs:
0

PSORTB (v3.0.2)

Localization:
Periplasmic
Score:
10

LocTree3

Localization:
periplasm
Score:
100

ClubSub-P

Localization:
Periplasmic

sosuiGramN

Localization:
unknown

PRED-TAT

Localization:
Sec signal peptide predicted. Most likely cleavage site: 1 - 20 [AQA-DD]
Score:
1

TatP

Prediction:
No signal peptide predicted.

Predict Now

Manual Curation in STEP

Comments

UniProt Name:
Ferrienterobactin-binding periplasmic protein
Localization:
Periplasmic
Notes:
-
References:
PMID: 10986237
Pseudogene/Mobile Element:
-

mRNA Expression

mRNA abundance (Taniguchi 2010)

mRNA molec/cell:
2

Protein Existence

Mass spectrometry experiments

Proteomics:
PMID: 20222674
Proteomics / Inner Membrane fraction:
-

Protein abundance/solubility

Protein abundance (Taniguchi 2010)

Protein molec/cell:
8
PaxDb:
53.5 ppm

show distribution

EcoCyc

Protein:
EG10294
Gene Locus:
fepB

Structural Annotation

SCOP

Class:
Alpha and beta proteins (a/b)
SCOP: 51349
Fold:
Chelatase-like
SCOP: 53799
Super Family:
Helical backbone metal receptor
SCOP: 53807

Protein Family Domains

Pfam domains

PFAM: P0AEL6

SMART database

SMART: fepB

Protein Complexes

Go to Complexes

Protein Hydrodynamics

Protein Solubility

Niwa et al 2009:
30 % PMID: 19251648

Heat-stability

Heat stable (85oC):
no PMID: 10806384

Disorder

Predict Disorder with IUPred tool:

Predict Now

Bioinformatic tools and predictions

SignalP 4.1

Signal Peptide:
Yes
Cleavage site amino acid:
27
Cleavage Score (Cmax):
0.581

LipoP 1.0

Prediction:
SpI
Cleavage site amino acid:
26
Score:
13.3736
Cleavage Rule:
-

TMHMM v2.0

Number of TMs:
0

Phobius

Signal Peptide:
Yes
Number of TMs:
0

PSORTB (v3.0.2)

Localization:
Periplasmic
Score:
10

LocTree3

Localization:
periplasm
Score:
100

ClubSub-P

Localization:
Periplasmic

sosuiGramN

Localization:
P (Periplasm)

PRED-TAT

Localization:
Sec signal peptide predicted. Most likely cleavage site: 1 - 26 [VQA-AD]
Score:
0.994

TatP

Prediction:
No signal peptide predicted.

Predict Now

Manual Curation in STEP

Comments

UniProt Name:
Negative regulator of flagellin synthesis (Anti-sigma-28 factor)
Localization:
Cytoplasmic, Extra-cellular
Notes:
Anti-sigma28 factor. FlgM is an anti-sigma factor that binds and inactivates sigma28 (FliA_Ecoli). Once the hook and basal body is assembled, FlgM is secreted by the flagellar export system thereby freeing sigma28 to activate transcription.
References:
PMID: 10322161 PMID: 12631703 PMID: 9988479
Pseudogene/Mobile Element:
-

mRNA Expression

mRNA abundance (Taniguchi 2010)

mRNA molec/cell:
-

Protein Existence

Mass spectrometry experiments

Proteomics:
PMID: 16606699 PMID: 20222674 PMID: 20803007
Proteomics / Inner Membrane fraction:
-

Protein abundance/solubility

Protein abundance (Taniguchi 2010)

Protein molec/cell:
-
PaxDb:
251 ppm

show distribution

EcoCyc

Protein:
EG12664
Gene Locus:
flgM

Structural Annotation

SCOP

Class:
All alpha proteins
SCOP: 46456
Fold:
Non-globular all-alpha subunits of globular proteins
SCOP: 48661
Super Family:
Anti-sigma factor FlgM
SCOP: 101498

Protein Family Domains

Pfam domains

PFAM: P0AEM4

SMART database

SMART: flgM

Protein Complexes

Go to Complexes

Protein Hydrodynamics

Protein Solubility

Niwa et al 2009:
89 % PMID: 19251648

Heat-stability

Heat stable (85oC):
no PMID: 10806384

Disorder

Predict Disorder with IUPred tool:

Predict Now

Bioinformatic tools and predictions

SignalP 4.1

Signal Peptide:
No
Cleavage site amino acid:
49
Cleavage Score (Cmax):
0.115

LipoP 1.0

Prediction:
CYT
Cleavage site amino acid:
0
Score:
-0.200913
Cleavage Rule:
-

TMHMM v2.0

Number of TMs:
0

Phobius

Signal Peptide:
No
Number of TMs:
0

PSORTB (v3.0.2)

Localization:
Cytoplasmic
Score:
9

LocTree3

Localization:
cytoplasm
Score:
76

ClubSub-P

Localization:
Uncertain

sosuiGramN

Localization:
unknown

PRED-TAT

Localization:
No signal peptide predicted
Score:
0.98

TatP

Prediction:
No signal peptide predicted.

Predict Now

E.coli K-12 (MG1655) Secretome

Distribution of sub-cellular topologies of this subproteome in K-12

311

Manual Curation in STEP

mRNA Expression

Protein Existence

Protein abundance/solubility

Structural Annotation

Protein Family Domains

Protein Hydrodynamics

Bioinformatic tools and predictions

Manual Curation in STEP

mRNA Expression

Protein Existence

Protein abundance/solubility

Structural Annotation

Protein Family Domains

Protein Hydrodynamics

Bioinformatic tools and predictions

Manual Curation in STEP

mRNA Expression

Protein Existence

Protein abundance/solubility

Structural Annotation

Protein Family Domains

Protein Hydrodynamics

Bioinformatic tools and predictions

Manual Curation in STEP

mRNA Expression

Protein Existence

Protein abundance/solubility

Structural Annotation

Protein Family Domains

Protein Hydrodynamics

Bioinformatic tools and predictions

Manual Curation in STEP

mRNA Expression

Protein Existence

Protein abundance/solubility

Structural Annotation

Protein Family Domains

Protein Hydrodynamics

Bioinformatic tools and predictions

Manual Curation in STEP

mRNA Expression

Protein Existence

Protein abundance/solubility

Structural Annotation

Protein Family Domains

Protein Hydrodynamics

Bioinformatic tools and predictions

Manual Curation in STEP

mRNA Expression

Protein Existence

Protein abundance/solubility

Structural Annotation

Protein Family Domains

Protein Hydrodynamics

Bioinformatic tools and predictions

Manual Curation in STEP

mRNA Expression

Protein Existence

Protein abundance/solubility

Structural Annotation

Protein Family Domains

Protein Hydrodynamics

Bioinformatic tools and predictions

Manual Curation in STEP

mRNA Expression

Protein Existence

Protein abundance/solubility

Structural Annotation

Protein Family Domains

Protein Hydrodynamics

Bioinformatic tools and predictions

Manual Curation in STEP

mRNA Expression

Protein Existence

Protein abundance/solubility

Structural Annotation