| entry | acc | gene | alt_gene | oln | protein_name | cm_loca | cm_notes | cm_refs | suboci_full | subloci | subloci_iim | nterm_cterm | bernsel | subloci_uniprot | subloci_echoL | organelle | target_chaperoning1 | 1st_ss | target_chaperoning2 | 2nd_ss | pseudo | common_core | basic_proteome | signalp_sp_exist | signalp_pos | signalp_cmax | lipop_pred | lipop_cleavage | lipop_score | lipop_rule | tmhmm | phobius_tms | phobius_sp_exist | phobius_prediction | psortb_loca | psortb_score | solubility |
| YMGJ_ECOLI | A5A612 | ymgJ | b4594 | periplasmic protein (SEC through IM), YmgJ | Weak signal peptide prediction. Function Unkown. | Periplasmic | G | IM (predicted) | - | SEC | no | yes | N | 24 | 0.144 | CYT | 0 | -0.200913 | - | 0 | 0 | Y | n6-17c22/23o | Unknown | 2 | ||||||||||||
| YJBT_ECOLI | A5A628 | yjbT | b4620 | periplasmic protein (SEC through IM), YjbT | Belongs to the yjbT family. | Periplasmic | G | N/A | - | SEC | no | yes | Y | 30 | 0.744 | SpI | 29 | 15.6556 | - | 1 | 0 | Y | n15-25c29/30o | Periplasmic | 9 | ||||||||||||
| RZOQ_ECOLI | C1P601 | rzoQ | b4689 JW1545.1 | IM lipoprotein (SEC through IM), inserted in OM by LOL), Putative RzoQ | Inner Membrane Lipoprotein | E | N/A | Cell membrane | - | SEC | LOL | no | yes | N | 29 | 0.129 | SpII | 22 | 11.4311 | Pos+2=V | 0 | 0 | Y | n9-20c25/26o | Unknown | 2 | |||||||||||
| YPDI_ECOLI | O32528 | ypdI | b2376 JW2373 | OM lipoprotein (SEC through IM, inserted in OM by LOL), YpdI | Outer Membrane Lipoprotein | I | N/A | Cell membrane | Outer membrane Lipoprotein | SEC | LOL | no | yes | Y | 24 | 0.415 | SpII | 18 | 12.5879 | Pos+2=N | 1 | 0 | Y | n5-16c23/24o | Unknown | 2 | 22 | ||||||||||
| YFJS_ECOLI | O52982 | yfjS | b2636 JW5921 | IM lipoprotein (SEC through IM, inserted in OM by LOL), YfjS | Inner Membrane Lipoprotein | E | N/A | Cell inner membrane | Inner membrane lipoprotein | SEC | LOL | Prophage Integrase (EcoGene) | no | no | Y | 21 | 0.479 | SpII | 20 | 18.7233 | Pos+2=D | 0 | 0 | Y | n6-17c21/22o | Unknown | 2 | ||||||||||
| SODM_ECOLI | P00448 | sodA | b3908 JW3879 | periplasmic protein (SEC through IM), Superoxide dismutase SodA | Periplasmic | The superoxide dismutase SodA is targeted to the periplasm in a SecA-dependent manner by a novel mechanism in Rhizobium leguminosarum. SodM and SodF are <i>E.coli</i> proteins that are close homoloques of the RISodA. SodA does not possess a hydrophobic cleaved N-terminal signal peptide typically present in soluble proteins exported by the Sec-dependent (Sec) pathway or the twin-arginine translocation (TAT) pathway. TatC or SecB mutants in Rhizobium leguminosarum do not affect export of SodA to the periplasm. Inhibition of SecA ATPase activity by azide blocks SodA export to the periplasm. | PMID: 21854464 | Periplasmic | G | N/A | Cytoplasmic | SEC | yes | yes | N | 51 | 0.145 | CYT | 0 | -0.200913 | - | 0 | 0 | N | i | Unknown | 5 | ||||||||||
| PPB_ECOLI | P00634 | phoA | b0383 JW0374 | periplasmic protein (SEC through IM), Alkaline phosphatase PhoA | Periplasmic | Substrate of periplasmic disulfide oxidoreductases DsbA. Alkaline phosphatase (EC 3.1.3.1; APase) of E.coli is a periplasmic enzyme. PMID: 3533724 Alkaline phosphatase (EC 3.1.3.1; APase) of E.coli is a periplasmic enzyme. PMID: 3533724 Identified DsbA substrate PMID: 19953543 PMID: 7022451 An amber mutation in the secA gene of Escherichia coli causes a pleiotropic decrease in the synthesis of secreted proteins. PMID: 3009404 Disulfide bridges (190 200;308 358) Disulfide bridges (190 200;308 358) Disulfide bond-containing protein PMID: 25664544 | PMID: 14726535 PMID: 19953543 PMID: 25664544 PMID: 3009404 PMID: 3533724 PMID: 7022451 | Periplasmic | G | N/A | Periplasm | Periplasmic (Experimental) | SEC | DSBA | no | yes | Y | 22 | 0.408 | SpI | 21 | 3.05238 | - | 0 | 0 | Y | n5-16c21/22o | Periplasmic | 10 | 78 | |||||||
| ASPG2_ECOLI | P00805 | ansB | b2957 JW2924 | periplasmic protein (SEC through IM), L-asparaginase 2 AnsB | Periplasmic | PMID: 2407723 PMID: 25403562 PMID: 25664544 PMID: 387570 PMID: 8434007 | Periplasmic | G | N/A | Periplasm | Periplasmic (Experimental) | SEC | yes | yes | Y | 23 | 0.82 | SpI | 22 | 19.7315 | - | 1 | 0 | Y | n7-17c22/23o | Periplasmic | 10 | 49 | |||||||||
| AMPC_ECOLI | P00811 | ampC ampA | ampA | b4150 JW4111 | periplasmic protein (SEC through IM), Beta-lactamase AmpC | Periplasmic | PMID: 6795623 | Periplasmic | G | N/A | Periplasm | Periplasmic (Experimental) | SEC | yes | yes | Y | 20 | 0.854 | SpI | 19 | 17.1519 | - | 0 | 0 | Y | n4-15c19/20o | Periplasmic | 10 | 26 | ||||||||
| ARAF_ECOLI | P02924 | araF | b1901 JW1889 | periplasmic protein (SEC through IM), L-arabinose-binding periplasmic AraF | Periplasmic | PMID: 9298646 | Periplasmic | G | N/A | Periplasm | Periplasmic (Experimental) | SEC | yes | yes | Y | 24 | 0.816 | SpI | 23 | 20.0244 | - | 0 | 0 | Y | n7-18c23/24o | Periplasmic | 10 | 86 | |||||||||
| RBSB_ECOLI | P02925 | rbsB prlB rbsP | prlB rbsP | b3751 JW3730 | periplasmic protein (SEC through IM), Ribose import binding RbsB | Periplasmic | Identified in periplasm by biochemical fractionation followed by two-dimensional gel electrophoresis and tandem mass spectrometry. PMID: 15911532 Potential Skp substrate PMID: 19953543 PMID: 9298646 | PMID: 15911532 PMID: 19953543 PMID: 9298646 | Periplasmic | G | Soluble IM Fraction | Periplasm | Periplasmic (Experimental) | SEC | SKP | no | yes | Y | 26 | 0.839 | SpI | 25 | 22.7093 | - | 0 | 0 | Y | n6-17c25/26o | Periplasmic | 10 | 93 | ||||||
| TOLC_ECOLI | P02930 | tolC colE1-i mtcB mukA refI | colE1-i mtcB mukA refI | b3035 JW5503 | OM b-barrel protein (SEC through IM, inserted in OM by BAM), TolC | Outer Membrane b-barrel protein | Part of MdtABC-TolC multidrug efflux transport system = [(TolC)3][MdtC][MdtB][MdtA]. Also part of MacAB-TolC macrolide efflux transport system = [(TolC)3][MacB]2[MacA]. Outer membrane protein determined by 2D gel electrophoresis (Lopez-Campistrous et al 2005, Fountoulakis et al 2003, Molloy et al 2000).The crystal structure of MacA has been determined at 3.0A resolution. The inner membrane transporter MacB requires the outer membrane factor TolC and the periplasmic adaptor protein MacA to form a functional tripartite complex. Electron microscopic indicates that the MacA hexamer interacts with TolCIn a tip-to-tip manner. Potential Skp substrate PMID: 19953543 PMID: 9298646 | PMID: 10806384 PMID: 10879525 PMID: 11266632 PMID: 12107133 PMID: 12624733 PMID: 15228545 PMID: 15911532 PMID: 16079137 PMID: 16352602 PMID: 16372265 PMID: 19254725 PMID: 19953543 PMID: 20932056 PMID: 21325274 PMID: 21778229 PMID: 9298646 | Outer Membrane b-barrel protein | H | Soluble IM Fraction | Cell outer membrane | Outer Membrane ??-barrel protein (Experimental) | SECB | SEC | SKP | BAM | yes | yes | Y | 23 | 0.712 | SpI | 22 | 10.2716 | - | 0 | 0 | Y | n4-14c22/23o | OuterMembrane | 10 | 15 | ||||
| OMPF_ECOLI | P02931 | ompF cmlB coa cry tolF | cmlB coa cry tolF | b0929 JW0912 | OM b-barrel protein (SEC through IM, inserted in OM by BAM), OmpF | Outer Membrane b-barrel protein | Substrate of periplasmic peptidyl prolyl cis???trans isomerase SurA. Potential pubstrate of periplasmic chaperones Skp. Outer membrane proteins OmpF, OmpC, and OmpX as central to the YebF export mechanism. Trigger factor-sensitive aggregation. Outer membrane protein determined by LC-MS (Gevaert et al 2002). Outer membrane protein determined by 2D gel electrophoresis (Lopez-Campistrous et al 2005, Fountoulakis et al 2003, Molloy et al 2000) Identified in nucleoids collected by sucrose gradient centrifugation and analyzed by liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS). PMID: 21541338 Potential Skp substrate PMID: 19953543 Identified SurA substrates PMID: 19953543 PMID: 9298646 | PMID: 10806384 PMID: 11266632 PMID: 12488465 PMID: 12624733 PMID: 1380671 PMID: 15911532 PMID: 16079137 PMID: 17908933 PMID: 19343722 PMID: 19737520 PMID: 19953543 PMID: 20932056 PMID: 21541338 PMID: 22658749 PMID: 8626309 PMID: 9298646 | Outer Membrane b-barrel protein | H | Soluble IM Fraction | Cell outer membrane | Outer Membrane ??-barrel protein (Experimental) | TF | SEC | SKP,SURA | BAM | yes | yes | Y | 23 | 0.883 | SpI | 22 | 19.9098 | - | 0 | 0 | Y | n6-17c22/23o | OuterMembrane | 10 | 18 | ||||
| PHOE_ECOLI | P02932 | phoE ompE | ompE | b0241 JW0231 | OM b-barrel protein (SEC through IM, inserted in OM by BAM), PhoE | Outer Membrane b-barrel protein | PMID: 11266632 PMID: 1380671 PMID: 20932056 PMID: 216015 | Outer Membrane b-barrel protein | H | N/A | Cell outer membrane; Multi-pass membrane protein | Outer Membrane ??-barrel protein (Experimental) | SEC | BAM | yes | yes | Y | 22 | 0.841 | SpI | 21 | 21.3546 | - | 0 | 0 | Y | n5-16c21/22o | OuterMembrane | 10 | 2 | |||||||
| LAMB_ECOLI | P02943 | lamB malB | malB | b4036 JW3996 | OM b-barrel protein (SEC through IM, inserted in OM by BAM), Maltoporin LamB | Outer Membrane b-barrel protein | Potential pubstrate of periplasmic chaperones Skp. Substrate of periplasmic peptidyl prolyl cis???trans isomerase SurA. Outer membrane protein determined by 2D gel electrophoresis (Lopez-Campistrous et al 2005, Fountoulakis et al 2003, Molloy 2000). Experimentally verified trimeric beta-barrel. N-terminal region is in periplasm. Potential Skp substrate PMID: 19953543 Identified SurA substrates PMID: 19953543 PMID: 346375 Disulfide bridges between 47 and 63 PMID: 1988451 Disulfide bond-containing protein PMID: 25664544 | PMID: 10806384 PMID: 11266632 PMID: 12624733 PMID: 15911532 PMID: 16079137 PMID: 16372265 PMID: 19343722 PMID: 1988451 PMID: 19953543 PMID: 20932056 PMID: 25664544 PMID: 346375 PMID: 7824948 PMID: 8626309 PMCID: PMC1993877 | Outer Membrane b-barrel protein | H | Soluble IM Fraction | Cell outer membrane | Outer Membrane ??-barrel protein (Experimental) | SEC | SKP,SURA,DEGP | BAM | yes | yes | Y | 26 | 0.795 | SpI | 25 | 20.7922 | - | 0 | 0 | Y | n8-18c25/26o | OuterMembrane | 10 | 54 | |||||
| MALM_ECOLI | P03841 | malM molA | molA | b4037 JW3997 | periplasmic protein (SEC through IM), Maltose operon periplasmic protein MalM | Periplasmic | PMID: 2434655 | Periplasmic | G | N/A | Periplasm | Periplasmic (Experimental) | SEC | yes | yes | Y | 27 | 0.606 | SpI | 26 | 24.5925 | - | 0 | 0 | Y | n7-18c26/27o | Periplasmic | 10 | |||||||||
| FIMA1_ECOLI | P04128 | fimA pilA | pilA | b4314 JW4277 | Fimbrial extra-cellular peripheral OM protein (SEC through IM, chaperone usher export system over OM), Type-1 protein, A chain FimA | Peripheral outer membrane protein facing the extra-cellular space | Fim operon (class ??1); Fimbrial Subunit. Experimentally verified signal peptide PMID: 2858471 Disulfide bridge (44 84) Disulfide bond-containing protein PMID: 25664544 | PMID: 20345943 PMID: 25664544 PMID: 2858471 PMID: 6147250 | Peripheral outer membrane protein facing the extra-cellular space | F4 | N/A | Fimbrium | Cell surface appendage (Experimental) | fimbriae | SEC | CU | yes | yes | Y | 24 | 0.78 | SpI | 23 | 25.7568 | - | 1 | 0 | Y | n5-16c23/24o | Extracellular | 10 | ||||||
| LIVK_ECOLI | P04816 | livK | b3458 JW3423 | periplasmic protein (SEC through IM), Leucine-specific-binding LivK | Periplasmic | Identified DsbA substrate PMID: 19953543 PMID: 9298646 Potential substrate for the SRP pathway. It was found to be down-regulated in the periplasmic compartment of SRP-depleted cells. PMID: 22030350 Disulfide bridge (76 101) Disulfide bridge (42 47) PMID: 8061609 Disulfide bond-containing protein PMID: 25664544 | PMID: 19953543 PMID: 22030350 PMID: 25664544 PMID: 8061609 PMID: 9298646 | Periplasmic | G | N/A | Periplasm | Periplasmic (Experimental) | SEC | DSBA | yes | yes | Y | 24 | 0.817 | SpI | 23 | 17.7516 | - | 0 | 0 | Y | n7-18c23/24o | Periplasmic | 10 | 78 | |||||||
| NLPA_ECOLI | P04846 | nlpA | b3661 JW3635 | IM lipoprotein (SEC through IM, inserted in OM by LOL), NlpA | Inner Membrane Lipoprotein | Proven signal peptidase II substrate. | PMID: 15174130 | Inner Membrane Lipoprotein | E | Soluble IM Fraction | Cell inner membrane; Lipid-anchor | Inner membrane lipoprotein | SEC | LOL | yes | yes | Y | 23 | 0.355 | SpII | 23 | 21.7987 | Pos+2=D | 0 | 0 | Y | n10-21c26/27o | CytoplasmicMembrane | 10 | 77 | |||||||
| FLIC_ECOLI | P04949 | fliC flaF hag | flaF hag | b1923 JW1908 | Flagellar extra-cellular peripheral OM protein (Flagellum secreted), Flagellin FliC | Peripheral outer membrane protein facing the extra-cellular space | Flameint protein, flagellin. Flagella proteins contribute to the production of outer membrane vesicles. Analysis of E. coli W3110 OMVs revealed that they contain abundant levels of FliC. Compared to the parental strain expressing wild-type fliC, an E. coli strain with a fliC-null mutation produced reduced amounts of OMVs based on both protein and phosphate levels. Identified in nucleoids collected by sucrose gradient centrifugation and analyzed by liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS). PMID: 21541338 PMID: 12730325 | PMID: 12730325 PMID: 21541338 PMID: 24134841 | Peripheral outer membrane protein facing the extra-cellular space | F4 | Soluble IM Fraction | Secreted Bacterial flagellum | Cell surface appendage (Experimental) | flagellum | Flagellum | Fla | no | yes | N | 52 | 0.13 | CYT | 0 | -0.200913 | - | 0 | 0 | N | i | Extracellular | 10 | 80 | |||||
| PTRA_ECOLI | P05458 | ptrA ptr | ptr | b2821 JW2789 | periplasmic protein (SEC through IM), Protease 3 PtrA | Periplasmic | Potential substrate of periplasmic peptidyl prolyl cis???trans isomerase SurA; sequence contains known binding motif of SurA [RYEF]X[RYEF][P]. PMID: 15502359 | PMID: 15502359 PMID: 18498364 | Periplasmic | G | N/A | Periplasm | Periplasmic(experimental) | SEC | SURA | yes | yes | Y | 24 | 0.815 | SpI | 23 | 13.0719 | - | 0 | 0 | Y | n9-18c23/24o | Periplasmic | 10 | 66 | ||||||
| FEPA_ECOLI | P05825 | fepA fep feuB | fep feuB | b0584 JW5086 | OM b-barrel protein (SEC through IM, inserted in OM by BAM), Ferrienterobactin receptor FepA | Outer Membrane b-barrel protein | Outer membrane protein determined by 2D gel electrophoresis (Lopez-Campistrous et al 2005, Fountoulakis et al 2003, Molloy et al 2000) Potential Skp substrate PMID: 19953543 PMID: 10529396 Disulfide bond-containing protein PMID: 25664544 | PMID: 10529396 PMID: 10806384 PMID: 11266632 PMID: 12624733 PMID: 19953543 PMID: 20932056 PMID: 25664544 PMID: 9886293 | Outer Membrane b-barrel protein | H | N/A | Cell outer membrane; Multi-pass membrane protein | Outer Membrane ??-barrel protein (Experimental) | SEC | SKP | BAM | yes | yes | Y | 23 | 0.836 | SpI | 22 | 11.5401 | - | 0 | 0 | Y | n7-17c22/23o | OuterMembrane | 10 | 11 | |||||
| BTUB_ECOLI | P06129 | btuB bfe cer dcrC | bfe cer dcrC | b3966 JW3938 | OM b-barrel protein (SEC through IM, inserted in OM by BAM), Vitamin B12 transporter BtuB | Outer Membrane b-barrel protein | Outer membrane protein determined by 2D gel electrophoresis (Lopez-Campistrous et al 2005, Fountoulakis et al 2003, Molloy et al 2000) Potential Skp substrate PMID: 19953543 PMID: 9298646 | PMID: 10806384 PMID: 11266632 PMID: 12595710 PMID: 12624733 PMID: 12652322 PMID: 15911532 PMID: 19953543 PMID: 2687240 PMID: 2982793 PMID: 9298646 | Outer Membrane b-barrel protein | H | N/A | Cell outer membrane | Outer Membrane ??-barrel protein (Experimental) | SEC | SKP | BAM | no | yes | Y | 21 | 0.828 | SpI | 20 | 21.8455 | - | 0 | 0 | Y | n5-16c20/21o | OuterMembrane | 10 | ||||||
| CDH_ECOLI | P06282 | cdh | b3918 JW3889 | periplasmic protein (SEC through IM), CDP-diacylglycerol pyrophosphatase Cdh | Periplasmic | G | Soluble IM Fraction | Cell inner membrane; Single-pass membrane protein | Membrane anchored | SEC | yes | yes | N | 19 | 0.264 | SpI | 19 | 7.43557 | - | 1 | 0 | Y | n4-14c19/20o | CytoplasmicMembrane | 10 | 55 | |||||||||||
| FHUA_ECOLI | P06971 | fhuA tonA | tonA | b0150 JW0146 | OM b-barrel protein (SEC through IM, inserted in OM by BAM), Ferrichrome transporter/phage receptor FhuA | Outer Membrane b-barrel protein | SecB substrate; Substrate of periplasmic peptidyl prolyl cis???trans isomerase SurA. Outer membrane protein determined by 2D gel electrophoresis (Lopez-Campistrous et al 2005, Fountoulakis et al 2003, Molloy et al 2000) Potential Skp substrate PMID: 19953543 Identified SurA substrates PMID: 19953543 PMID: 3079747 Disulfide bridge (351 362;725 731) Disulfide bond-containing protein PMID: 25664544 | PMID: 10806384 PMID: 11266632 PMID: 12624733 PMID: 15911532 PMID: 16352602 PMID: 19343722 PMID: 19953543 PMID: 25664544 PMID: 3079747 PMID: 9856937 | Outer Membrane b-barrel protein | H | N/A | Cell outer membrane | Outer Membrane ??-barrel protein (Experimental) | SECB | SEC | SURA,SKP | BAM | yes | yes | Y | 34 | 0.805 | SpI | 33 | 20.4987 | - | 0 | 0 | Y | n16-24c33/34o | OuterMembrane | 10 | 7 | ||||
| OMPC_ECOLI | P06996 | ompC meoA par | meoA par | b2215 JW2203 | OM b-barrel protein (SEC through IM, inserted in OM by BAM), OmpC | Outer Membrane b-barrel protein | Outer membrane protein related to resistance to ampicillin and tetracycline; Potential pubstrate of periplasmic chaperones Skp. Outer membrane proteins OmpF, OmpC, and OmpX as central to the YebF export mechanism. Trigger factor-sensitive aggregation. Outer membrane protein determined by LC-MS (Gevaert et al 2002). Outer membrane protein determined by 2D gel electrophoresis (Lopez-Campistrous et al 2005, Fountoulakis et al 2003, Molloy et al 2000) Identified in nucleoids collected by sucrose gradient centrifugation and analyzed by liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS). PMID: 21541338 Potential Skp substrate PMID: 19953543 PMID: 9298646 | PMID: 10806384 PMID: 12488465 PMID: 12624733 PMID: 15911532 PMID: 16372265 PMID: 17908933 PMID: 19737520 PMID: 19953543 PMID: 21541338 PMID: 22658749 PMID: 9298646 | Outer Membrane b-barrel protein | H | Soluble IM Fraction | Cell outer membrane; Multi-pass membrane protein | Outer Membrane ??-barrel protein (Experimental) | TF | SEC | SKP | BAM | yes | yes | Y | 22 | 0.884 | SpI | 21 | 21.1188 | - | 0 | 0 | Y | n5-16c21/22o | OuterMembrane | 10 | 6 | ||||
| YFIB_ECOLI | P07021 | yfiB | b2605 JW2586 | OM lipoprotein (SEC through IM), inserted in OM by LOL), Putative YfiB | Outer Membrane Lipoprotein | Proven pignal peptidase II substrate. PMID: 15174130 | PMID: 15174130 | Outer Membrane Lipoprotein | I | N/A | Cell membrane | Outer membrane Lipoprotein (Experimental) | SEC | LOL | yes | yes | N | 25 | 0.134 | SpII | 18 | 18.8761 | Pos+2=Q | 0 | 0 | Y | n5-16c20/21o | OuterMembrane | 9 | 74 | |||||||
| USHA_ECOLI | P07024 | ushA | b0480 JW0469 | periplasmic protein (SEC through IM), UshA | Periplasmic | PMID: 25664544 PMID: 9298646 | Periplasmic | G | Soluble IM Fraction | Periplasm | Periplasmic (Experimental) | SEC | no | yes | Y | 26 | 0.748 | SpI | 25 | 11.3752 | - | 0 | 0 | Y | n7-18c25/26o | Periplasmic | 10 | 77 | |||||||||
| PPA_ECOLI | P07102 | appA | b0980 JW0963 | periplasmic protein (SEC through IM), AppA | Periplasmic | Identified DsbC substrate PMID: 19953543 PMID: 8387749 Disulfide bridge (99 130;155 430;200 210;404 413) Disulfide bond-containing protein PMID: 25664544 | PMID: 19953543 PMID: 25664544 PMID: 8387749 | Periplasmic | G | N/A | Periplasm | Periplasmic (Experimental) | SEC | DSBC | yes | yes | Y | 23 | 0.793 | SpI | 22 | 13.6965 | - | 0 | 0 | Y | n3-13c22/23o | Periplasmic | 10 | 66 | |||||||
| FHUD_ECOLI | P07822 | fhuD | b0152 JW0148 | periplasmic protein (TAT-SEC through IM), Iron(3+)-hydroxamate-binding FhuD | Periplasmic | TAT secretion system. PMID: 8522527 | PMID: 17218314 PMID: 8522527 | Periplasmic | G | N/A | Periplasm | Periplasmic (Experimental) | TAT,SEC | yes | yes | Y | 31 | 0.749 | SpI | 30 | 8.81274 | - | 0 | 0 | Y | n12-23c30/31o | Periplasmic | 10 | |||||||||
| FIMF_ECOLI | P08189 | fimF | b4318 JW4281 | Fimbrial extra-cellular peripheral OM protein (SEC through IM, chaperone usher export system over OM), FimF | Peripheral outer membrane protein facing the extra-cellular space | Fim operon (class ??1); Fimbrial Subunit. Required for the regulation of length and mediation of adhesion of Escherichia coli type 1 fimbriae. PMID: 15372038 Disulfide bridge (38 78) Disulfide bond-containing protein PMID: 25664544 | PMID: 15372038 PMID: 19820722 PMID: 20345943 PMID: 25664544 PMID: 2890081 | Peripheral outer membrane protein facing the extra-cellular space | F4 | N/A | Fimbrium | Cell surface appendage (Experimental) | fimbriae | SEC | CU | no | yes | Y | 23 | 0.871 | SpI | 22 | 17.2366 | - | 0 | 0 | Y | n6-17c22/23o | Extracellular | 9 | |||||||
| FIMG_ECOLI | P08190 | fimG | b4319 JW4282 | Fimbrial extra-cellular peripheral OM protein (SEC through IM, chaperone usher export system over OM), FimG | Peripheral outer membrane protein facing the extra-cellular space | Fim operon (class ??1); Fimbrial Subunit. Required for the regulation of length and mediation of adhesion of Escherichia coli type 1 fimbriae. PMID: 15372038 Disulfide bridge (39 77) Disulfide bond-containing protein PMID: 25664544 | PMID: 15372038 PMID: 19820722 PMID: 20345943 PMID: 25664544 PMID: 2890081 | Peripheral outer membrane protein facing the extra-cellular space | F4 | N/A | Fimbrium | Cell surface appendage (Experimental) | fimbriae | SEC | CU | no | yes | Y | 24 | 0.773 | SpI | 23 | 18.1281 | - | 1 | 0 | Y | n7-18c23/24o | Extracellular | 9 | 73 | ||||||
| FIMH_ECOLI | P08191 | fimH | b4320 JW4283 | Fimbrial extra-cellular peripheral OM protein (SEC through IM, chaperone usher export system over OM), Type 1 fimbrin D-mannose specific adhesin FimH | Peripheral outer membrane protein facing the extra-cellular space | Fim operon (class ??1); Potential tip-adhesin. Required for the regulation of length and mediation of adhesion of Escherichia coli type 1 fimbriae. PMID: 1971261 PMID: 2900235 PMID: 2890081 | PMID: 1971261 PMID: 19820722 PMID: 20345943 PMID: 24140205 PMID: 2890081 PMID: 2900235 | Peripheral outer membrane protein facing the extra-cellular space | F4 | N/A | Fimbrium | Cell surface appendage (Experimental) | fimbriae | SEC | CU | no | yes | Y | 20 | 0.539 | SpI | 21 | 14.3197 | - | 0 | 0 | Y | n4-14c19/20o | Unknown | 4 | 19 | ||||||
| CPDB_ECOLI | P08331 | cpdB | b4213 JW4171 | periplasmic protein (SEC through IM), 2',3'-cyclic-nucleotide 2'-phosphodiesterase/3'-nucleotidase CpdB | Periplasmic | Potential substrate of periplasmic peptidyl prolyl cis???trans isomerase SurA; sequence contains known binding motif of SurA [RYEF]X[RYEF][P]. References: Identified in periplasm by biochemical fractionation followed by two-dimensional gel electrophoresis and tandem mass spectrometry. PMID: 15911532 PMID: 9298646 Disulfide bond-containing protein PMID: 25664544 | PMID: 15911532 PMID: 18498364 PMID: 25664544 PMID: 3005231 PMID: 9298646 | Periplasmic | G | N/A | Periplasm | Periplasmic (Experimental) | SEC | SURA | no | yes | Y | 20 | 0.787 | SpI | 19 | 14.5002 | - | 0 | 0 | Y | n4-14c19/20o | Periplasmic | 10 | 82 | |||||||
| YGDB_ECOLI | P08370 | ygdB | b2824 JW5450 | periplasmic protein (SEC through IM), YgdB | Periplasmic | G | N/A | Periplasmic | SEC | yes | yes | N | 27 | 0.147 | SpI | 26 | 7.55205 | - | 1 | 0 | Y | n10-23c35/36o | Unknown | 2 | 7 | ||||||||||||
| DACC_ECOLI | P08506 | dacC | b0839 JW0823 | periplasmic peripheral IM protein (SEC through IM), D-alanyl-D-alanine carboxypeptidase DacC | Peripheral inner membrane protein facing the periplasm | Putative amphiphilic alpha-helical anchor at the C-terminusPMID: 8162192. PMID: 9858669 DacC(PBP-6) anchors to the periplasmic face of the inner membrane via C-terminal amphiphilic alpha-helix. The Alpha-helical conformation in the C-terminal anchoring domain is observed in aqueous solution or in the presence of phospholipid vesicles. PMID: 9371419 | PMID: 8162192 PMID: 9371419 PMID: 9858669 | Peripheral inner membrane protein facing the periplasm | F2 | Soluble IM Fraction | Cell inner membrane | Membrane anchored (Experimental) | SEC | yes | yes | Y | 28 | 0.887 | SpI | 27 | 18.4084 | - | 0 | 0 | Y | n11-22c27/28o | CytoplasmicMembrane | 9 | 21 | ||||||||
| OMPT_ECOLI | P09169 | ompT | b0565 JW0554 | OM b-barrel protein (SEC through IM, inserted in OM by BAM), Protease 7 OmpT | Outer Membrane b-barrel protein | SecB substrate. Trigger factor-sensitive aggregation. Outer membrane protein determined by 2D gel electrophoresis (Lopez-Campistrous et al 2005, Fountoulakis et al 2003, Molloy et al 2000) Identified in nucleoids collected by sucrose gradient centrifugation and analyzed by liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS). PMID: 21541338 PMID: 9298646 | PMID: 10806384 PMID: 11266632 PMID: 11566868 PMID: 12624733 PMID: 15911532 PMID: 16352602 PMID: 19737520 PMID: 21541338 PMID: 9298646 | Outer Membrane b-barrel protein | H | Soluble IM Fraction | Cell outer membrane; Multi-pass membrane protein | Outer Membrane ??-barrel protein (Experimental) | SECB,TF | SEC | BAM | Prophage Integrase (EcoGene) | no | no | Y | 21 | 0.295 | SpI | 20 | 7.49489 | - | 0 | 0 | Y | n5-16c20/21o | OuterMembrane | 10 | 3 | |||||
| GLPQ_ECOLI | P09394 | glpQ | b2239 JW2233 | periplasmic protein (SEC through IM), Glycerophosphodiester phosphodiesterase, periplasmic GlpQ | Periplasmic | PMID: 1851953 PMID: 2829735 | Periplasmic | G | N/A | Periplasm | Periplasmic (Experimental) | SEC | yes | yes | Y | 26 | 0.712 | SpI | 25 | 17.875 | - | 0 | 0 | Y | n8-18c25/26o | Periplasmic | 10 | 39 | |||||||||
| ARGT_ECOLI | P09551 | argT | b2310 JW2307 | periplasmic protein (SEC through IM), Lysine/arginine/ornithine-binding periplasmic ArgT | Periplasmic | This periplasmic binding protein is involved in an arginine transport system. ArgT and histidine-binding protein J (hisJ) interact with a common membrane-bound receptor, hisP. Phosphorylated by ArgKPMID: 2136858. Identified DsbA substrate PMID: 19953543 PMID: 9298647 PMID: 9298646 Disulfide bridge (60 67) Disulfide bond-containing protein PMID: 25664544 | PMID: 19953543 PMID: 2136858 PMID: 25664544 PMID: 9298646 PMID: 9298647 | Periplasmic | G | N/A | Periplasm | Periplasmic (Experimental) | SEC | yes | yes | Y | 23 | 0.595 | SpI | 22 | 19.5206 | - | 0 | 0 | Y | n4-14c22/23o | Periplasmic | 10 | |||||||||
| ENO_ECOLI | P0A6P9 | eno | b2779 JW2750 | cytoplasmic peripheral IM protein facing the cytoplasm & peripheral OM protein facing the extra-cellular space (SEC through IM), Enolase Eno | Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm, Peripheral outer membrane protein facing the extra-cellular space | When modified at Lys342 possibly secreted by an unknown mechanism, no evidence of signal peptide) Homodimer, stabilized by Mg2+; Catalyzes the reversible conversion of 2-phosphoglycerate into phosphoenolpyruvate. Essential for the degradation of carbohydrates via glycolysis. Also a component of the RNA degradosome. The covalent binding to the substrate at Lys-342 causes inactivation of the enzyme, and possibly serves as a signal for the export of the protein. Interacts with the C-terminal region of the endoribonuclease RNase EIn the RNA degradosome. Fractions of enolase are present in both the cytoplasm and on the cell surface. As part of the bacterial cytoskeleton in the cytoplasm,Is organized as extended coiled structures that wind around the cell, from one cell pole to the other. When covalently bound to the substrate at Lys-342, the inactive enolase is secreted, and remains attached to the bacterial cell surface. PMID: 8610017 PMID: 9600841 PMID: 9740056 PMID: 9732527 PMID: 12035760 Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). PMID: 23230279 | PMID: 12035760 PMID: 15003462 PMID: 17242352 PMID: 18337249 PMID: 23230279 PMID: 8610017 PMID: 9600841 PMID: 9732527 PMID: 9740056 | Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm, Peripheral outer membrane protein facing the extra-cellular space | A, F1, F4 | Soluble IM Fraction | Cytoplasm; cytoskeleton Secreted Cell surface | Cytoplasmic (Experimental) | SEC | yes | yes | N | 42 | 0.139 | CYT | 0 | -0.200913 | - | 0 | 0 | N | i | Cytoplasmic | 9 | 100 | ||||||||
| FLGH_ECOLI | P0A6S0 | flgH fla FVIII flaY | fla FVIII flaY | b1079 JW5153 | Flagellar OM lipoprotein (SEC through IM), inserted in OM by LOL), L-ring FlgH | Outer Membrane Lipoprotein | Proven pignal peptidase II substrate. L ring protein. PMID: 15174130 | PMID: 12631703 PMID: 15174130 PMID: 20932056 | Outer Membrane Lipoprotein | I | N/A | Cell outer membrane | Outer membrane Lipoprotein (Experimental) | flagellum | SEC | LOL | yes | yes | Y | 24 | 0.235 | SpII | 21 | 17.0713 | Pos+2=A | 0 | 0 | Y | n14-25c33/34o | OuterMembrane | 10 | 24 | |||||
| FLGI_ECOLI | P0A6S3 | flgI fla FIX flaM | fla FIX flaM | b1080 JW1067 | Flagellar periplasmic protein (SEC through IM), P-ring FlgI | Periplasmic | P ring protein. Substrate of periplasmic disulfide oxidoreductases DsbA. Might be is co-translationally translocated through Sec translocon. Identified DsbA substrate PMID: 19953543 PMID: 12730325 Potential substrate for the SRP pathway. When fused to cytoplasmic protein thioredoxin promotes signal recognition particle-dependent translocation PMID: 15838024 | PMID: 12631703 PMID: 12730325 PMID: 14726535 PMID: 15838024 PMID: 19953543 | Periplasmic | G | N/A | Periplasm Bacterial flagellum basal body | Periplasmic (Experimental) | flagellum | SRP (exp) | SEC | DSBA | Fla | no | yes | Y | 20 | 0.783 | SpI | 19 | 15.6262 | - | 0 | 0 | Y | n4-14c19/20o | Periplasmic | 10 | 63 | |||
| TOLB_ECOLI | P0A855 | tolB | b0740 JW5100 | periplasmic & peripheral OM protein (SEC through IM), Tol-Pal system TolB | Periplasmic, Peripheral outer membrane protein facing the periplasm | SecB substrate. Might be is co-translationally translocated through Sec translocon. PMID: 17233825 PMID: 7929011 Potential substrate for the SRP pathway. When fused to cytoplasmic protein thioredoxin promotes signal recognition particle-dependent translocation PMID: 15838024 | PMID: 15838024 PMID: 16352602 PMID: 17233825 PMID: 7929011 | Periplasmic, Peripheral outer membrane protein facing the periplasm | G, F3 | Soluble IM Fraction | Periplasm | Periplasmic (Experimental) | SECB, SRP | SEC | no | yes | N | 22 | 0.555 | SpI | 21 | 7.66433 | - | 0 | 0 | Y | n7-16c21/22o | Periplasmic | 10 | 31 | |||||||
| FLIE_ECOLI | P0A8T5 | fliE fla AI flaN | fla AI flaN | b1937 JW1921 | Flagellar periplasmic peripheral IM protein (Flagellum secreted through IM), hook-basal body complex FliE | Peripheral inner membrane protein facing the periplasm | MS-ring rod junction protein | PMID: 10809679 | Peripheral inner membrane protein facing the periplasm | F2 | N/A | Bacterial flagellum basal body | Cytoplasmic | flagellum | Flagellum | no | yes | N | 41 | 0.269 | CYT | 0 | -0.200913 | - | 0 | 0 | N | i | Periplasmic | 10 | 62 | ||||||
| YCEI_ECOLI | P0A8X2 | yceI | b1056 JW1043 | periplasmic protein (SEC through IM), YceI | Periplasmic | PMID: 22030350 PMID: 9298646 | Periplasmic | G | N/A | Periplasm | Periplasmic (Experimental) | SEC | yes | yes | Y | 23 | 0.827 | SpI | 22 | 23.1728 | - | 0 | 0 | Y | n4-15c22/23o | Unknown | 2 | 69 | |||||||||
| YCCT_ECOLI | P0A8X4 | yccT | b0964 JW0947 | periplasmic protein (SEC through IM), UPF0319 YccT | Periplasmic | G | N/A | Periplasmic | SEC | yes | yes | Y | 21 | 0.531 | SpI | 20 | 6.4525 | - | 0 | 0 | Y | n3-14c20/21o | Unknown | 2 | 60 | ||||||||||||
| BLC_ECOLI | P0A901 | blc yjeL | yjeL | b4149 JW4110 | OM lipoprotein (SEC through IM, inserted in OM by LOL), Blc | Outer Membrane Lipoprotein | Proven pignal peptidase II substrate. Identified in nucleoids collected by sucrose gradient centrifugation and analyzed by liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS). PMID: 21541338 PMID: 15174130 | PMID: 15174130 PMID: 20932056 PMID: 21541338 | Outer Membrane Lipoprotein | I | N/A | Cell outer membrane; Lipid-anchor | Outer membrane Lipoprotein (Experimental) | SEC | LOL | yes | yes | Y | 19 | 0.216 | SpII | 18 | 14.624 | Pos+2=S | 0 | 0 | Y | n3-15c20/21o | OuterMembrane | 10 | 52 | ||||||
| BAMC_ECOLI | P0A903 | bamC dapX nlpB | dapX nlpB | b2477 JW2462 | OM lipoprotein (SEC through IM, inserted in OM by LOL), assembly factor BamC | Outer Membrane Lipoprotein | Outer membrane protein related to resistance to ampicillin and tetracycline. Part of bam complex. Forms subcomplex with BamCE. Proven pignal peptidase II substrate. Outer membrane protein determined by LC-MS (Gevaert et al 2002). Outer membrane protein determined by 2D gel electrophoresis (Lopez-Campistrous et al 2005, Fountoulakis et al 2003, Molloy et al 2000) Identified in nucleoids collected by sucrose gradient centrifugation and analyzed by liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS). PMID: 21541338 PMID: 10806384 | PMID: 10806384 PMID: 12488465 PMID: 12624733 PMID: 15174130 PMID: 15911532 PMID: 16079137 PMID: 16372265 PMID: 20932056 PMID: 21139201 PMID: 21370981 PMID: 21541338 PMID: 21823654 | Outer Membrane Lipoprotein | I | Soluble IM Fraction | Cell outer membrane | Outer membrane Lipoprotein (Experimental) | SEC | LOL | yes | yes | N | 32 | 0.177 | SpII | 24 | 17.2458 | Pos+2=S | 0 | 0 | Y | n13-23c27/28o | OuterMembrane | 10 | 86 | ||||||
| SLYB_ECOLI | P0A905 | slyB | b1641 JW1633 | OM lipoprotein (SEC through IM, inserted in OM by LOL), SlyB | Outer Membrane Lipoprotein | Proven pignal peptidase II substrate. Outer membrane protein determined by 2D gel electrophoresis (Lopez-Campistrous et al 2005, Molloy et al 2000) Identified in nucleoids collected by sucrose gradient centrifugation and analyzed by liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS). PMID: 21541338 PMID: 10806384 | PMID: 10806384 PMID: 15174130 PMID: 15911532 PMID: 20932056 PMID: 21541338 | Outer Membrane Lipoprotein | I | Soluble IM Fraction | Cell outer membrane | Outer membrane Lipoprotein (Experimental) | SEC | LOL | yes | yes | N | 22 | 0.181 | SpII | 17 | 19.9051 | Pos+2=V | 0 | 0 | Y | n5-16c21/22o | OuterMembrane | 9 | ||||||||
| MIPA_ECOLI | P0A908 | mipA yeaF | yeaF | b1782 JW1771 | OM b-barrel protein (SEC through IM, inserted in OM by BAM), MltA-interacting protein MipA | Outer Membrane b-barrel protein | MipA is considered to be a structural protein mediating the assembly of MltA to PBP1B into a complexPMID: 10037771. MipA couples both enzymes to one another in vivo, it is likely to give rise to a membrane adhesion site as depicted. Such contacts between the membranes. Outer membrane protein determined by 2D gel electrophoresis (Lopez-Campistrous et al 2005, Fountoulakis et al 2003, Molloy 2000,Thein 2010)PMID: 15911532PMID: 10806384PMID: 12624733PMID: 20932056 PMID: 10037771 | PMID: 10037771 PMID: 10806384 PMID: 12624733 PMID: 15911532 PMID: 16079137 PMID: 20932056 | Outer Membrane b-barrel protein | H | N/A | Cell outer membrane | Outer Membrane ??-barrel protein (Experimental) | SEC | BAM | yes | yes | Y | 23 | 0.835 | SpI | 22 | 21.2923 | - | 0 | 0 | Y | n6-17c22/23o | OuterMembrane | 10 | 11 | ||||||
| OMPA_ECOLI | P0A910 | ompA con tolG tut | con tolG tut | b0957 JW0940 | OM b-barrel protein (SEC through IM, inserted in OM by BAM), OmpA | Outer Membrane b-barrel protein | Substrate of periplasmic peptidyl prolyl cis???trans isomerase SurA. Potential pubstrate of periplasmic chaperones Skp. Trigger factor-sensitive aggregation. Trigger factor-sensitive aggregation. Copurifies with trigger factor in pull down assays. The Lipid Bilayer-Inserted Membrane Protein BamA of Escherichia coli Facilitates Insertion and Folding of Outer Membrane Protein A from Its Complex with Skp. Outer membrane protein determined by LC-MS (Gevaert et al 2002). Outer membrane protein determined by 2D gel electrophoresis (Lopez-Campistrous et al 2005, Fountoulakis et al 2003, Molloy et al 2000) Identified in nucleoids collected by sucrose gradient centrifugation and analyzed by liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS). PMID: 21541338 Potential Skp substrate PMID: 19953543 Identified SurA substrates PMID: 19953543 Identified DsbA substrate PMID: 19953543 PMID: 9298646 SecA is sufficient to transport a secretory protein with hydrophobic signal sequence across SecYEG, but efficient translocation requires SRP in Addition PMID: 24717922 Disulfide bridge (311 323) Disulfide bond-containing protein PMID: 25664544 | PMID: 10806384 PMID: 11266632 PMID: 12488465 PMID: 12624733 PMID: 16079137 PMID: 17908933 PMID: 19343722 PMID: 19737520 PMID: 19953543 PMID: 20932056 PMID: 21541338 PMID: 21778229 PMID: 23641708 PMID: 24717922 PMID: 25664544 PMID: 7813480 PMID: 8626309 PMID: 9298646 PMID: 9808047 | Outer Membrane b-barrel protein | H | Soluble IM Fraction | Cell outer membrane | Outer Membrane ??-barrel protein (Experimental) | TF | SEC | SKP,SURA,DEGP,DSBA | BAM | yes | yes | Y | 22 | 0.872 | SpI | 21 | 28.1613 | - | 0 | 0 | Y | n5-16c21/22o | OuterMembrane | 10 | 10 | ||||
| PAL_ECOLI | P0A912 | pal excC | excC | b0741 JW0731 | OM lipoprotein (SEC through IM), inserted in OM by LOL), PG-associated Pal | Outer Membrane Lipoprotein | Proven pignal peptidase II substrate. Outer membrane protein determined by 2D gel electrophoresis (Lopez-Campistrous et al 2005, Fountoulakis et al 2003, Molloy et al 2000) PMID: 10806384 PMID: 9298646 | PMID: 10806384 PMID: 12624733 PMID: 15174130 PMID: 15911532 PMID: 19364805 PMID: 20932056 PMID: 23193105 PMID: 9298646 | Outer Membrane Lipoprotein | I | Soluble IM Fraction | Cell outer membrane | Outer membrane Lipoprotein (Experimental) | SEC | LOL | yes | yes | Y | 29 | 0.5 | SpII | 21 | 21.6668 | Pos+2=S | 0 | 0 | Y | n6-14c18/19o | OuterMembrane | 10 | 81 | ||||||
| OMPW_ECOLI | P0A915 | ompW yciD | yciD | b1256 JW1248 | OM b-barrel protein (SEC through IM, inserted in OM by BAM), OmpW | Outer Membrane b-barrel protein | Outer membrane protein related to resistance to ampicillin and tetracycline. Outer membrane protein determined by 2D gel electrophoresis (Lopez-Campistrous et al 2005, Fountoulakis et al 2003, Molloy et al 2000) PMID: 9298646 | PMID: 10806384 PMID: 11266632 PMID: 12624733 PMID: 15911532 PMID: 16372265 PMID: 16414958 PMID: 20932056 PMID: 9298646 | Outer Membrane b-barrel protein | H | N/A | Cell outer membrane | Outer Membrane ??-barrel protein (Experimental) | SEC | BAM | yes | yes | Y | 22 | 0.754 | SpI | 21 | 26.4914 | - | 0 | 0 | Y | n4-15c21/22o | OuterMembrane | 10 | 42 | ||||||
| OMPX_ECOLI | P0A917 | ompX ybiG | ybiG | b0814 JW0799 | OM b-barrel protein (SEC through IM, inserted in OM by BAM), OmpX | Outer Membrane b-barrel protein | SecB substrate. Substrate of periplasmic peptidyl prolyl cis???trans isomerase SurA. Outer membrane proteins OmpF, OmpC, and OmpX as central to the YebF export mechanism. Trigger factor-sensitive aggregation. Outer membrane protein determined by LC-MS (Gevaert et al 2002). Outer membrane protein determined by 2D gel electrophoresis (Lopez-Campistrous et al 2005, Molloy et al 2000) Identified in nucleoids collected by sucrose gradient centrifugation and analyzed by liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS). PMID: 21541338 Potential Skp substrate PMID: 19953543 Identified SurA substrates PMID: 19953543 PMID: 9298646 | PMID: 10545325 PMID: 10806384 PMID: 11266632 PMID: 12488465 PMID: 15911532 PMID: 16352602 PMID: 19343722 PMID: 19737520 PMID: 19953543 PMID: 20932056 PMID: 21541338 PMID: 22658749 PMID: 9298646 | Outer Membrane b-barrel protein | H | Soluble IM Fraction | Cell outer membrane; Multi-pass membrane protein | Outer Membrane ??-barrel protein (Experimental) | SECB,TF | SEC | SURA,SKP | BAM | yes | yes | Y | 24 | 0.63 | SpI | 23 | 26.0753 | - | 1 | 0 | Y | n4-15c23/24o | OuterMembrane | 10 | 14 | ||||
| PA1_ECOLI | P0A921 | pldA | b3821 JW3794 | OM b-barrel protein (SEC through IM, inserted in OM by BAM), Phospholipase A1 PldA | Outer Membrane b-barrel protein | Outer membrane protein determined by 2D gel electrophoresis (Lopez-Campistrous et al 2005, Fountoulakis et al 2003) Potential Skp substrate PMID: 19953543 PMID: 6397464 | PMID: 10537112 PMID: 10806384 PMID: 11266632 PMID: 12624733 PMID: 19953543 PMID: 20932056 PMID: 6397463 PMID: 6397464 | Outer Membrane b-barrel protein | H | N/A | Cell outer membrane | Outer Membrane ??-barrel protein (Experimental) | SEC | SKP | BAM | yes | yes | Y | 21 | 0.528 | SpI | 20 | 7.74532 | - | 0 | 0 | Y | n3-14c20/21o | OuterMembrane | 10 | 9 | ||||||
| TSX_ECOLI | P0A927 | tsx nupA | nupA | b0411 JW0401 | OM b-barrel protein (SEC through IM, inserted in OM by BAM), Nucleoside-specific channel-forming Tsx | Outer Membrane b-barrel protein | Outer membrane protein related to resistance to ampicillin and tetracycline. Outer membrane protein determined by 2D gel electrophoresis (Lopez-Campistrous et al 2005, Fountoulakis et al 2003, Molloy et al 2000) Potential Skp substrate PMID: 19953543 PMID: 10806384 | PMID: 10806384 PMID: 12624733 PMID: 15911532 PMID: 16372265 PMID: 19953543 | Outer Membrane b-barrel protein | H | Soluble IM Fraction | Cell outer membrane | Outer Membrane ??-barrel protein (Experimental) | SEC | BAM | Prophage Transposase | yes | no | Y | 23 | 0.882 | SpI | 22 | 24.8185 | - | 0 | 0 | Y | n4-14c22/23o | OuterMembrane | 10 | 0 | |||||
| WZA_ECOLI | P0A930 | wza | b2062 JW2047 | OM lipoprotein & extra-cellular peripheral OM protein (SEC through IM), inserted in OM by LOL), Putative polysaccharide export Wza | Outer Membrane Lipoprotein, Peripheral outer membrane protein facing the extra-cellular space | Has a helical structure that crosses membrane. The transmembrane region of Wza is an alpha-helical barrel. Proven pignal peptidase II substrate. It has been demostrated that it can be also secreted and be surface exposed. PMID: 15174130 | PMID: 10619844 PMID: 15174130 PMCID: PMC3315050 | Outer Membrane Lipoprotein, Peripheral outer membrane protein facing the extra-cellular space | I, F4 | N/A | Cell outer membrane | Outer membrane Lipoprotein (Experimental) | SEC | LOL | yes | yes | Y | 26 | 0.13 | SpII | 20 | 12.3938 | Pos+2=T | 0 | 0 | Y | n11-21c25/26o | OuterMembrane | 9 | 44 | |||||||
| GFCE_ECOLI | P0A932 | gfcE yccZ | yccZ | b0983 JW0966 | OM lipoprotein (SEC through IM), inserted in OM by LOL), Putative polysaccharide export GfcE | Outer Membrane Lipoprotein | Proven pignal peptidase II substrate. PMID: 15174130 | PMID: 15174130 PMID: 20932056 | Outer Membrane Lipoprotein | I | N/A | Cell outer membrane | Outer membrane Lipoprotein (Experimental) | SEC | LOL | yes | yes | Y | 23 | 0.202 | SpII | 20 | 15.093 | Pos+2=T | 0 | 0 | Y | n10-21c26/27o | OuterMembrane | 9 | 59 | ||||||
| MLTA_ECOLI | P0A935 | mltA mlt ygdM | mlt ygdM | b2813 JW2784 | OM lipoprotein (SEC through IM), inserted in OM by LOL), Membrane-bound lytic murein transglycosylase A MltA | Outer Membrane Lipoprotein | The formation of a complex between a murein polymerase (PBP1B) and a murein hydrolase (MltA). In the presence of MipA represents a first step in a reconstitution of the hypothetical murein-synthesizing holoenzyme. Postulated to be responsible for controlled growth of the stress-bearing sacculus of <i>E.coli</i>. Proven pignal peptidase II substrate. Outer membrane protein determined by 2D gel electrophoresis (Lopez-Campistrous et al 2005, Fountoulakis et al 2003) PMID: 15174130 SRP recognizes the first signal anchor sequence of a polytopic membrane protein (MtlA) resulting in cotranslational targeting of MtlA to SecY and phospholipids of the plasma membrane. PMID: 10619852 Possible interaction of SRP with the secretory protein pOmpA is prevented by the association of trigger factor with nascent pOmpA. PMID: 10619852 | PMID: 10037771 PMID: 10619852 PMID: 12624733 PMID: 15174130 PMID: 20932056 | Outer Membrane Lipoprotein | I | N/A | Cell outer membrane; Lipid-anchor | Outer membrane Lipoprotein (Experimental) | SEC | LOL | pseudogene (Ochman & Davalos, 2006) | no | yes | Y | 20 | 0.191 | SpII | 20 | 22.7365 | Pos+2=S | 0 | 0 | Y | n9-19c23/24o | OuterMembrane | 10 | 35 | |||||
| BAME_ECOLI | P0A937 | bamE smpA | smpA | b2617 JW2598 | OM lipoprotein (SEC through IM, inserted in OM by LOL), assembly factor BamE | Outer Membrane Lipoprotein | Part of BAM complex. Forms subcomplex with BamCE. | PMID: 17404237 PMID: 20932056 PMID: 21139201 PMID: 21370981 PMID: 21823654 | Outer Membrane Lipoprotein | I | Soluble IM Fraction | Cell outer membrane | Outer membrane Lipoprotein | SEC | LOL | yes | yes | Y | 24 | 0.197 | SpII | 19 | 19.5033 | Pos+2=S | 0 | 0 | Y | n5-16c20/21o | OuterMembrane | 9 | 44 | ||||||
| BAMA_ECOLI | P0A940 | bamA yaeT yzzN yzzY | yaeT yzzN yzzY | b0177 JW0172 | OM b-barrel protein (SEC through IM, inserted in OM by BAM), assembly factor BamA | Outer Membrane b-barrel protein | Part of bam complexPMID: 21823654PMID: 21370981. Folding of OmpA from its complex with the periplasmic chaperone Skp into bilayers composed of phosphatidylethanolamine and phosphatidylcholine was inhibited in the absence of BamA but facilitated when BamA was present, indicating an interaction of Skp-OmpA complexes with BamAPMID: 23641708. Outer membrane protein determined by 2D gel electrophoresis (PMID: 15911532,PMID: 12624733,PMID: 20932056) Potential Skp substrate PMID: 19953543 PMID: 9298646 | PMID: 10806384 PMID: 12624733 PMID: 16079137 PMID: 19525348 PMID: 19953543 PMID: 20932056 PMID: 21370981 PMID: 21823654 PMID: 23641708 PMID: 9298646 | Outer Membrane b-barrel protein | H | Soluble IM Fraction | Cell outer membrane | Outer Membrane ss-barrel protein(experimental) | SEC | BAM | yes | yes | Y | 21 | 0.641 | SpI | 20 | 10.5737 | - | 0 | 0 | Y | n6-13c20/21o | OuterMembrane | 10 | 32 | ||||||
| YOBA_ECOLI | P0AA57 | yobA | b1841 JW1830 | periplasmic protein (SEC through IM), YobA | Periplasmic | G | N/A | Periplasm | Periplasmic | SEC | yes | yes | Y | 27 | 0.511 | SpI | 26 | 13.4937 | - | 0 | 0 | Y | n11-21c26/27o | Periplasmic | 9 | ||||||||||||
| YEAY_ECOLI | P0AA91 | yeaY | b1806 JW1795 | OM lipoprotein (SEC through IM, inserted in OM by LOL), YeaY | Outer Membrane Lipoprotein | Proven pignal peptidase II substrate. PMID: 15174130 | PMID: 15174130 PMID: 20932056 | Outer Membrane Lipoprotein | I | N/A | Cell membrane | Outer membrane Lipoprotein (Experimental) | SEC | LOL | yes | yes | N | 27 | 0.138 | SpII | 22 | 14.0614 | Pos+2=V | 0 | 0 | Y | n10-20c25/26o | OuterMembrane | 9 | 25 | |||||||
| YACC_ECOLI | P0AA95 | yacC | b0122 JW0118 | periplasmic protein (SEC through IM), YacC | Periplasmic | G | N/A | Periplasmic | SEC | yes | yes | Y | 23 | 0.239 | SpI | 24 | 10.6167 | - | 0 | 0 | Y | n7-17c22/23o | Unknown | 2 | 21 | ||||||||||||
| YAFK_ECOLI | P0AA99 | yafK | b0224 JW0214 | periplasmic protein (SEC through IM), Putative L,D-transpeptidase YafK | Periplasmic | G | N/A | Periplasmic | SEC | yes | yes | Y | 20 | 0.74 | SpI | 19 | 10.678 | - | 1 | 0 | Y | n4-22c27/28o | Unknown | 2 | 25 | ||||||||||||
| ECPA_ECOLI | P0AAA3 | ecpA matB yagZ | matB yagZ | b0293 JW0287 | Fimbrial periplasmic protein (SEC through IM), Common pilus major fimbrillin subunit EcpA | Periplasmic | Putative chaperone???usher fimbrial operon yag (class ??); Fimbrial Subunit (yagZ). PMID: 16522795 | PMID: 16522795 PMID: 20345943 | Periplasmic | G | N/A | Fimbrium | Periplasmic (Experimental) | fimbriae | SEC | no | yes | Y | 23 | 0.863 | SpI | 22 | 25.6343 | - | 0 | 0 | Y | n5-15c22/23o | Unknown | 2 | 34 | ||||||
| ZRAP_ECOLI | P0AAA9 | zraP yjaI zra | yjaI zra | b4002 JW5546 | periplasmic protein (SEC through IM), Zinc resistance-associated protein ZraP | Periplasmic | PMID: 9694902 | Periplasmic | G | N/A | Periplasm | Periplasmic (Experimental) | SEC | yes | yes | Y | 27 | 0.74 | SpI | 26 | 28.6968 | - | 1 | 0 | Y | n7-18c26/27o | Periplasmic | 10 | |||||||||
| YNFG_ECOLI | P0AAJ1 | ynfG | b1589 JW1581 | periplasmic peripheral IM protein (TAT via piggyback through IM), Probable anaerobic dimethyl sulfoxide reductase chain YnfG | Peripheral inner membrane protein facing the periplasm | The physiological substrate and relevance of the YnfFGH S- and N-oxide reductase is unknown. DmsB paralog. So far, hydroxy pyridine N-oxide is the best substrate for YnfFGH. ynfFGH are expressed anaerobically. YnfG can function with DmsAC, replacing DmsB a as the electron transfer subunit. The ynfEFGHI operon is a paralogue of the Escherichia coli dmsABC operon. ynfE and ynfF are paralogues of dmsA. ynfG and ynfH are paralogues of dmsB and dmsC, respectively. PMID: 14522592 | PMID: 14522592 | Peripheral inner membrane protein facing the periplasm | F2 | N/A | Periplasmic | TAT (piggyback) | yes | yes | N | 44 | 0.106 | CYT | 0 | -0.200913 | - | 0 | 0 | N | i | Unknown | 5 | 38 | |||||||||
| HYBA_ECOLI | P0AAJ8 | hybA | b2996 JW2964 | periplasmic peripheral IM protein (TAT-SEC through IM), Hydrogenase-2 operon HybA | Peripheral inner membrane protein facing the periplasm | TAT secretion system. Part of membrane-associated hydrogenase 4 complex. PMID: 9738917 | PMID: 15911532 PMID: 21890895 PMID: 22313414 PMID: 9738917 | Peripheral inner membrane protein facing the periplasm | F2 | IM (predicted) | Periplasm | Periplasmic (Experimental) | TAT,SEC | yes | yes | Y | 27 | 0.442 | SpI | 26 | 19.7463 | - | 0 | 1 | Y | n9-20c26/27o293-311i | CytoplasmicMembrane | 9 | 39 | ||||||||
| NRFC_ECOLI | P0AAK7 | nrfC yjcJ | yjcJ | b4072 JW4033 | periplasmic protein (TAT through IM), NrfC | Periplasmic | TAT secretion system. PMID: 15280383 | PMID: 15280383 PMID: 17218314 | Periplasmic | G | N/A | Periplasmic (Experimental) | TAT | yes | yes | N | 28 | 0.447 | SpI | 27 | 7.45987 | - | 0 | 0 | Y | n8-19c27/28o | Cytoplasmic | 10 | 23 | ||||||||
| NAPG_ECOLI | P0AAL3 | napG yojA yojB | yojA yojB | b2205 JW2193 | periplasmic protein (TAT through IM), Ferredoxin-type NapG | Periplasmic | TAT secretion system. PMID: 11967083 | PMID: 11967083 PMID: 17218314 | Periplasmic | G | N/A | Periplasm | Periplasmic (Experimental) | TAT | yes | yes | N | 42 | 0.316 | SpI | 41 | 0.19393 | - | 0 | 0 | Y | n21-32c41/42o | Cytoplasmic | 8 | 20 | |||||||
| YKGJ_ECOLI | P0AAL9 | ykgJ | b0288 JW0282 | periplasmic protein (SEC through IM), YkgJ | Periplasmic | Periplasmic | G | N/A | Periplasmic | SEC | no | yes | N | 27 | 0.286 | SpI | 24 | 4.7292 | - | 0 | 0 | N | i | Unknown | 2 | 33 | |||||||||||
| YBAV_ECOLI | P0AAR8 | ybaV | b0442 JW0432 | periplasmic protein (SEC through IM), YbaV | Periplasmic | G | N/A | Periplasmic | SEC | yes | yes | Y | 26 | 0.681 | SpI | 25 | 23.157 | - | 0 | 0 | Y | n7-17c25/26o | CytoplasmicMembrane | 9 | |||||||||||||
| YBGS_ECOLI | P0AAV6 | ybgS | b0753 JW0736 | periplasmic protein (SEC through IM), YbgS | Periplasmic | Disulfide bond-containing protein PMID: 25664544 | PMID: 25664544 | Periplasmic | G | N/A | Periplasmic | SEC | yes | yes | Y | 25 | 0.845 | SpI | 24 | 32.7676 | - | 0 | 0 | Y | n6-17c24/25o | Periplasmic | 9 | ||||||||||
| YBIJ_ECOLI | P0AAX3 | ybiJ | b0802 JW0787 | periplasmic protein (SEC through IM), YbiJ | Periplasmic | G | N/A | Periplasm | Periplasmic | SEC | yes | yes | Y | 23 | 0.811 | SpI | 22 | 21.8351 | - | 1 | 0 | Y | n7-18c22/23o | Unknown | 2 | ||||||||||||
| MCBA_ECOLI | P0AAX6 | mcbA ybiM | ybiM | b0806 JW5106 | periplasmic protein (SEC through IM), McbA | Periplasmic | G | N/A | Periplasm | Periplasmic | SEC | yes | yes | Y | 23 | 0.869 | SpI | 22 | 18.838 | - | 1 | 0 | Y | n4-14c22/23o | Unknown | 2 | |||||||||||
| YBIS_ECOLI | P0AAX8 | ybiS | b0819 JW0803 | periplasmic peripheral OM protein (SEC through IM), Probable L,D-transpeptidase YbiS | Peripheral outer membrane protein facing the periplasm | Potential substrate of periplasmic peptidyl prolyl cis???trans isomerase SurA; sequence contains known binding motif of SurA [RYEF]X[RYEF][P]. YbiS has been shown to crosslink Braun's lipoprotein (Lpp) to the peptidoglycan. PMID: 9298646 Potential substrate for the SRP pathway. It was found to be down-regulated in the periplasmic compartment of SRP-depleted cells. PMID: 22030350 | PMID: 18498364 PMID: 22030350 PMID: 23832002 PMID: 9298646 | Peripheral outer membrane protein facing the periplasm | F3 | N/A | Periplasm | Periplasmic (Experimental) | SEC | SURA | yes | yes | Y | 25 | 0.584 | SpI | 24 | 19.8413 | - | 1 | 0 | Y | n8-19c24/25o | Unknown | 2 | 40 | |||||||
| YBJH_ECOLI | P0AAY4 | ybjH | b0843 JW0827 | periplasmic protein (SEC through IM), YbjH | Periplasmic | G | N/A | Periplasmic | SEC | yes | yes | Y | 23 | 0.874 | SpI | 22 | 25.9172 | - | 1 | 0 | Y | n6-17c22/23o | Unknown | 2 | 10 | ||||||||||||
| YCBK_ECOLI | P0AB06 | ycbK | b0926 JW0909 | periplasmic protein (TAT through IM), YcbK | Periplasmic | TAT secretion system. | PMID: 17218314 | Periplasmic | G | N/A | Periplasmic | TAT | yes | yes | N | 31 | 0.559 | SpI | 30 | 8.29508 | - | 1 | 0 | Y | n11-22c30/31o | Unknown | 2 | 11 | |||||||||
| YMBA_ECOLI | P0AB10 | ymbA | b0952 JW5127 | IM lipoprotein (SEC through IM), inserted in OM by LOL), Intertransport PqiC YmbA | putative protase (enterobacterium homolog) | Inner Membrane Lipoprotein | E | N/A | Cell outer membrane | Cytoplasmic | SEC | LOL | yes | yes | N | 20 | 0.205 | SpII | 15 | 22.8198 | Pos+2=S | 0 | 0 | Y | n4-14c19/20o | Unknown | 2 | 82 | |||||||||
| EFEO_ECOLI | P0AB24 | efeO ycdO | ycdO | b1018 JW1003 | periplasmic protein (SEC through IM), Iron uptake system component EfeO | Periplasmic | Identified DsbA substrate PMID: 19953543 PMID: 12107143 Potential substrate for the SRP pathway. It was found to be down-regulated in the periplasmic compartment of SRP-depleted cells. PMID: 22030350 Disulfide bond-containing protein PMID: 25664544 | PMID: 12107143 PMID: 16551627 PMID: 19953543 PMID: 22030350 PMID: 25664544 | Periplasmic | G | N/A | Periplasm | Periplasmic (Experimental) | SEC | DSBA | yes | yes | Y | 27 | 0.767 | SpI | 26 | 18.2235 | - | 0 | 0 | Y | n9-18c26/27o | Periplasmic | 10 | 42 | ||||||
| YCEB_ECOLI | P0AB26 | yceB | b1063 JW1050 | OM lipoprotein (SEC through IM, inserted in OM by LOL), YceB | Outer Membrane Lipoprotein | Proven pignal peptidase II substrate. PMID: 15174130 | PMID: 15174130 | Outer Membrane Lipoprotein | I | Soluble IM Fraction | Cell membrane | Outer membrane Lipoprotein (Experimental) | SEC | LOL | yes | yes | N | 55 | 0.134 | SpII | 18 | 16.4921 | Pos+2=N | 0 | 0 | Y | n4-15c19/20o | Unknown | 2 | 45 | |||||||
| YCEK_ECOLI | P0AB31 | yceK | b1050 JW5151 | OM lipoprotein (SEC through IM, inserted in OM by LOL), YceK | Outer Membrane Lipoprotein | Proven pignal peptidase II substrate. PMID: 15174130 | PMID: 15174130 | Outer Membrane Lipoprotein | I | N/A | Outer membrane Lipoprotein (Experimental) | SEC | LOL | no | yes | N | 31 | 0.129 | SpII | 15 | 5.35698 | Pos+2=G | 0 | 0 | Y | n3-13c18/19o | Unknown | 2 | |||||||||
| LPOB_ECOLI | P0AB38 | lpoB ycfM | ycfM | b1105 JW5157 | OM lipoprotein (SEC through IM), inserted in OM by LOL), Penicillin-binding activator LpoB | Outer Membrane Lipoprotein | The two OM Lipoproteins, LpoA and LpoB, are essential for the function, respectively, of PBP1A and PBP1B, the major E. coli bifunctional peptidoglycans (PG) synthases. Each Lpo protein binds specifically to its cognate PBP and stimulates its transpeptidase activity, thereby facilitating attachment of new peptidoglycans to the PG sacculus. Proven pignal peptidase II substrate. PMID: 15174130 | PMID: 15174130 PMID: 20932056 PMID: 21183073 PMID: 21183074 | Outer Membrane Lipoprotein | I | N/A | Cell outer membrane | Outer membrane Lipoprotein (Experimental) | SEC | LOL | yes | yes | Y | 24 | 0.258 | SpII | 19 | 23.7759 | Pos+2=V | 0 | 0 | Y | n8-18c22/23o | Unknown | 2 | 96 | ||||||
| BHSA_ECOLI | P0AB40 | bhsA comC ycfR | comC ycfR | b1112 JW1098 | periplasmic peripheral OM protein (SEC through IM), Multiple stress resistance BhsA | Peripheral outer membrane protein facing the periplasm | By membrane fractionation, ComC was shown to be localized in the outer membrane. | PMID: 22089859 | Peripheral outer membrane protein facing the periplasm | F3 | N/A | Cell outer membrane | Periplasmic | SEC | yes | yes | Y | 23 | 0.773 | SpI | 22 | 21.6297 | - | 0 | 0 | Y | n6-14c22/23o | Unknown | 2 | 70 | |||||||
| YMGD_ECOLI | P0AB46 | ymgD | b1171 JW5177 | periplasmic protein (SEC through IM), YmgD | Periplasmic | Disulfide bond-containing protein PMID: 25664544 | PMID: 25664544 | Periplasmic | G | N/A | Periplasmic | SEC | no | yes | Y | 20 | 0.656 | SpI | 19 | 14.7848 | - | 0 | 0 | Y | n4-14c19/20o | Unknown | 2 | 86 | |||||||||
| APBE_ECOLI | P0AB85 | apbE yojK yojL | yojK yojL | b2214 JW5368 | IM lipoprotein (SEC through IM), inserted in OM by LOL), FAD:FMN transferase ApbE | Inner Membrane Lipoprotein | Proven signal peptidase II substrate. | PMID: 15174130 | Inner Membrane Lipoprotein | E | IM (predicted) | Cell inner membrane | Inner membrane lipoprotein | SEC | LOL | yes | yes | N | 28 | 0.154 | SpII | 19 | 12.0176 | Pos+2=D | 0 | 0 | Y | n8-18c22/23o | Unknown | 2 | 65 | ||||||
| CSGB_ECOLI | P0ABK7 | csgB | b1041 JW1024 | Curli related extra-cellular peripheral OM protein (SEC through IM), secreted over OM by curli), Minor curlin subunit CsgB | Peripheral outer membrane protein facing the extra-cellular space | Curli component. CsgB localization requires at least one component of the curli secretion apparatus, CsgF. Curli are a functional amyloid whose in vivo polymerization requires a dedicated nucleator protein, CsgB, and an assembly protein, CsgF. Without CsgF, curli subunits are released from the cell into the media and are inefficiently polymerized, resulting in fewer and mislocalized curli fibers. PMID: 19131513 PMID: 9383186 PMID: 9312041 | PMID: 16704339 PMID: 19131513 PMID: 21645131 PMID: 9312041 PMID: 9383186 PMCID: PMC2630086 | Peripheral outer membrane protein facing the extra-cellular space | F4 | N/A | Fimbrium | Cell surface appendage (Experimental) | Curli | SEC | Curli | yes | yes | Y | 22 | 0.53 | SpI | 21 | 16.29 | - | 0 | 0 | Y | n5-13c21/22o | Extracellular | 9 | |||||||
| NRFA_ECOLI | P0ABK9 | nrfA | b4070 JW4031 | periplasmic protein (SEC through IM), Cytochrome c-552 NrfA | Periplasmic | Disulfide bond-containing protein PMID: 25664544 | PMID: 25664544 PMID: 793493 | Periplasmic | G | N/A | Periplasm | Periplasmic (Experimental) | SEC | yes | yes | Y | 27 | 0.665 | SpI | 26 | 14.744 | - | 0 | 0 | Y | n11-21c26/27o | Periplasmic | 10 | 25 | ||||||||
| NRFB_ECOLI | P0ABL1 | nrfB yjcI | yjcI | b4071 JW4032 | periplasmic protein (SEC through IM), Cytochrome c-type NrfB | Periplasmic | Disulfide bond-containing protein PMID: 25664544 | PMID: 15280383 PMID: 25664544 | Periplasmic | G | N/A | Periplasm | Periplasmic (Experimental) | SEC | no | yes | Y | 33 | 0.498 | SpI | 25 | 6.62799 | - | 0 | 0 | Y | n7-18c23/24o | Periplasmic | 9 | 16 | |||||||
| NAPB_ECOLI | P0ABL3 | napB yejY | yejY | b2203 JW5367 | periplasmic protein (SEC through IM), nitrate reductase, electron transfer subunit NapB | Periplasmic | Nitrate reductase, napFDAGHBC, is located in the periplasm. E. coli also expresses two membrane-bound proton-translocating nitrate reductases, encoded by the narGHJI and narZYWV operons. PMID: 11844760 PMID: 10234835 Disulfide bond-containing protein PMID: 25664544 | PMID: 10234835 PMID: 10548535 PMID: 11844760 PMID: 17130127 PMID: 25664544 | Periplasmic | G | N/A | Periplasm | Periplasmic (Experimental) | SEC | yes | yes | Y | 28 | 0.825 | SpI | 27 | 14.6891 | - | 0 | 0 | Y | n9-20c27/28o | Periplasmic | 10 | ||||||||
| SFMA_ECOLI | P0ABW5 | sfmA | b0530 JW0519 | Fimbrial periplasmic protein (SEC through IM), SfmA | Periplasmic | Potential fimbrial sfm operon (class ??1); Fimbrial Subunit. Disulfide bridge (41 81) | PMID: 19820722 PMID: 20345943 | Periplasmic | G | N/A | Fimbrium | Periplasmic | fimbriae | SEC | CU | yes | yes | Y | 23 | 0.832 | SpI | 22 | 21.4596 | - | 0 | 0 | Y | n6-17c22/23o | Extracellular | 10 | 83 | ||||||
| FLGB_ECOLI | P0ABW9 | flgB fla FII flbA | fla FII flbA | b1073 JW1060 | Flagellar periplasmic protein (Flagellum secreted through IM), basal body rod FlgB | Periplasmic | Proximal rod protein. PMID: 12730325 FlgG is a distal component of the basal body rod. While FlgF was highly soluble, FlgB, FlgC, FlgG and FliE tended to self or cross-aggregate into fibrils in solutions at neutral pH or below, at high ionic strength, or at high protein concentration. PMID: 15136044 | PMID: 12631703 PMID: 12730325 PMID: 15136044 | Periplasmic | G | N/A | Bacterial flagellum basal body | Periplasmic (Experimental) | flagellum | Flagellum | Fla | yes | yes | N | 34 | 0.201 | CYT | 0 | -0.200913 | - | 0 | 0 | N | i | Periplasmic | 10 | 93 | |||||
| FLGC_ECOLI | P0ABX2 | flgC fla FIII flaW | fla FIII flaW | b1074 JW1061 | Flagellar periplasmic protein (Flagellum secreted through IM), basal body rod FlgC | Periplasmic | Proximal rod protein. PMID: 12730325 FlgB, FlgC, FlgF, and FlgG are four proteins that comprise the rod section of the basal-body assembly of the flagellar motor. The rod is a rotational component which is situated transverse to the outer membrane and peptidoglycan layers and is circumscribed by a set of four rings which lie in a horizontal plane within the inner and outer membranes of the cell envelope. PMID: 2181149 FlgG is a distal component of the basal body rod. While FlgF was highly soluble, FlgB, FlgC, FlgG and FliE tended to self or cross-aggregate into fibrils in solutions at neutral pH or below, at high ionic strength, or at high protein concentration. PMID: 15136044 | PMID: 12631703 PMID: 12730325 PMID: 15136044 PMID: 2181149 | Periplasmic | G | N/A | Bacterial flagellum basal body | Periplasmic (Experimental) | flagellum | Flagellum | Fla | yes | yes | N | 32 | 0.201 | CYT | 0 | -0.200913 | - | 0 | 0 | N | i | Periplasmic | 10 | 43 | |||||
| FLGG_ECOLI | P0ABX5 | flgG fla FVII flaL | fla FVII flaL | b1078 JW1065 | Flagellar periplasmic protein (Flagellum secreted through IM), basal body rod FlgG | Periplasmic | Distal rod protein PMID: 12730325 FlgB, FlgC, FlgF, and FlgG are four proteins that comprise the rod section of the basal-body assembly of the flagellar motor. The rod is a rotational component which is situated transverse to the outer membrane and peptidoglycan layers and is circumscribed by a set of four rings which lie in a horizontal plane within the inner and outer membranes of the cell envelope. PMID: 2181149 FlgG is a distal component of the basal body rod. While FlgF was highly soluble, FlgB, FlgC, FlgG and FliE tended to self or cross-aggregate into fibrils in solutions at neutral pH or below, at high ionic strength, or at high protein concentration. PMID: 15136044 | PMID: 12631703 PMID: 12730325 PMID: 15136044 PMID: 2181149 | Periplasmic | G | N/A | Bacterial flagellum basal body | Periplasmic (Experimental) | flagellum | Flagellum | Fla | yes | yes | N | 24 | 0.123 | CYT | 0 | -0.200913 | - | 0 | 0 | N | i | Extracellular | 10 | 78 | |||||
| SURA_ECOLI | P0ABZ6 | surA | b0053 JW0052 | periplasmic protein (SEC through IM), Chaperone SurA | Periplasmic | PMID: 14506253 PMID: 19525348 PMID: 21370981 PMID: 22030350 PMID: 9298646 | Periplasmic | G | N/A | Periplasm | Periplasmic (Experimental) | SEC | yes | yes | Y | 21 | 0.795 | SpI | 20 | 15.5107 | - | 0 | 0 | Y | n7-15c20/21o | Periplasmic | 10 | 86 | |||||||||
| BAMD_ECOLI | P0AC02 | bamD yfiO | yfiO | b2595 JW2577 | OM lipoprotein (SEC through IM, inserted in OM by LOL), assembly factor BamD | Outer Membrane Lipoprotein | Part of bam complex. Forms subcomplex with BamCE. Outer membrane protein related to resistance to ampicillin and tetracycline. YfiO stabilizes the YaeT complex and is essential for outer membrane protein assembly in Escherichia coli. Outer membrane protein determined by LC-MS (Gevaert et al 2002). Outer membrane protein determined by 2D gel electrophoresis (Lopez-Campistrous et al 2005) PMID: 16372265 | PMID: 12488465 PMID: 15174130 PMID: 15911532 PMID: 16079137 PMID: 16372265 PMID: 16824102 PMID: 20932056 PMID: 21139201 PMID: 21370981 PMID: 21823654 | Outer Membrane Lipoprotein | I | Soluble IM Fraction | Cell outer membrane | Outer membrane Lipoprotein(experimental) | SEC | LOL | yes | yes | Y | 24 | 0.171 | SpII | 19 | 27.0165 | Pos+2=S | 0 | 0 | Y | n7-17c22/23o | OuterMembrane | 10 | 22 | ||||||
| MBHM_ECOLI | P0ACE0 | hybC | b2994 JW2962 | periplasmic peripheral IM protein (TAT via piggyback through IM), Hydrogenase-2 large chain HybC | Peripheral inner membrane protein facing the periplasm | The protein is attached to this location through a 5kDa segment of its small subunit hybO that is has a TAT signal peptide. Secreted using the TAT system (piggyback). PMID: 10224080 An active tryptic fragment of membrane-bound hydrogenase isoenzyme 2 from anaerobically grown Escherichia coli has been purified. This indicates that HYD2 is attached to the periplasmic side of the cytoplasmic membrane.PMID: 3516690 The intracellular availability of nickel is essential for the processing of the large subunit and for the transmembrane translocation of HYD2. PMID: 8772179 PMID: 9738917 Disulfide bond-containing protein PMID: 25664544 | PMID: 10224080 PMID: 25664544 PMID: 3516690 PMID: 8772179 PMID: 9738917 | Peripheral inner membrane protein facing the periplasm | F2 | Soluble IM Fraction | Cell membrane; Peripheral membrane protein | Periplasmic (Experimental) | TAT (piggyback) | yes | yes | N | 34 | 0.139 | CYT | 0 | -0.200913 | - | 0 | 0 | N | i | CytoplasmicMembrane | 10 | 28 | ||||||||
| HOKC_ECOLI | P0ACG4 | hokC gef | gef | b4412 JW5002 b0018.1 | periplasmic protein (SEC through IM), Toxic HokC | Periplasmic | Potentially toxic protein. | PMID: 19121005 PMID: 21890895 | Periplasmic | G | N/A | Cell inner membrane | Periplasmic | SEC | no | yes | N | 22 | 0.361 | SpI | 21 | 5.3199 | - | 1 | 0 | Y | n8-16c21/22o | Periplasmic | 9 | 32 | |||||||
| HOKD_ECOLI | P0ACG6 | hokD relF | relF | b1562 JW1554 | periplasmic protein (SEC through IM), Toxic HokD | Periplasmic | G | N/A | Cell inner membrane | Periplasmic | SEC | Prophage Integrase (EcoGene) | no | no | N | 23 | 0.157 | SpI | 22 | 2.62207 | - | 1 | 0 | Y | n8-19c26/27o | Periplasmic | 9 | ||||||||||
| YDBJ_ECOLI | P0ACW2 | ydbJ | b4529 JW5215 | OM lipoprotein (SEC through IM, inserted in OM by LOL), YdbJ | Outer Membrane Lipoprotein | I | N/A | Outer membrane Lipoprotein | SEC | LOL | no | yes | Y | 25 | 0.374 | SpII | 16 | 17.2226 | Pos+2=S | 0 | 0 | Y | n3-14c19/20o | Unknown | 2 | 24 | |||||||||||
| YDCA_ECOLI | P0ACW4 | ydcA | b1419 JW1416 | periplasmic protein (SEC through IM), YdcA | Periplasmic | Disulfide bond-containing protein PMID: 25664544 | PMID: 25664544 | Periplasmic | G | N/A | Periplasmic | SEC | no | yes | Y | 21 | 0.761 | SpI | 20 | 12.9463 | - | 0 | 0 | Y | n4-15c20/21o | Unknown | 2 | ||||||||||
| YFHG_ECOLI | P0AD44 | yfhG | b2555 JW2539 | OM lipoprotein (TAT-SEC through IM, inserted in OM by LOL), YfhG | Outer Membrane Lipoprotein | TAT secretion system. | PMID: 17218314 | Outer Membrane Lipoprotein | I | N/A | Outer membrane Lipoprotein | TAT,SEC | LOL | yes | yes | Y | 23 | 0.159 | SpII | 25 | 6.96106 | Pos+2=V | 0 | 0 | Y | n13-24c29/30o | Unknown | 2 | 55 | ||||||||
| IVY_ECOLI | P0AD59 | ivy ykfE | ykfE | b0220 JW0210 | periplasmic protein (SEC through IM), Inhibitor of vertebrate lysozyme Ivy | Periplasmic | Experimentally verified gene-product. Verified that the signal peptide is cleaved. PMID: 11278658 Disulfide bridge (85 90) Disulfide bond-containing protein PMID: 25664544 | PMID: 11278658 PMID: 25664544 PMID: 9868784 | Periplasmic | G | Soluble IM Fraction | Periplasm | Periplasmic (Experimental) | SEC | yes | yes | Y | 29 | 0.849 | SpI | 28 | 16.6514 | - | 0 | 0 | Y | n13-23c28/29o | Periplasmic | 10 | 27 | |||||||
| LIVJ_ECOLI | P0AD96 | livJ | b3460 JW3425 | periplasmic protein (SEC through IM), Leu/Ile/Val-binding LivJ | Periplasmic | Substrate of periplasmic disulfide oxidoreductases DsbA. Identified DsbA substrate PMID: 19953543 PMID: 9298646 Potential substrate for the SRP pathway. It was found to be down-regulated in the periplasm and up-regulated in the cytoplasm of SRP-depleted cells. PMID: 22030350 Disulfide bridge (76 101) Disulfide bond-containing protein PMID: 25664544 | PMID: 14726535 PMID: 19953543 PMID: 22030350 PMID: 25664544 PMID: 9298646 | Periplasmic | G | N/A | Periplasm | Periplasmic (Experimental) | SEC | DSBA | yes | yes | Y | 24 | 0.832 | SpI | 23 | 20.6668 | - | 0 | 0 | Y | n7-18c23/24o | Periplasmic | 10 | 96 | |||||||
| TESA_ECOLI | P0ADA1 | tesA apeA pldC | apeA pldC | b0494 JW0483 | periplasmic protein (SEC through IM), Thioesterase 1/protease 1/lysophospholipase L1 TesA | Periplasmic | PMID: 8098033 | Periplasmic | G | N/A | Periplasm | Periplasmic (Experimental) | SEC | no | yes | N | 27 | 0.484 | SpI | 26 | 11.0906 | - | 1 | 0 | Y | n12-22c26/27o | Periplasmic | 10 | 40 | ||||||||
| NLPD_ECOLI | P0ADA3 | nlpD | b2742 JW2712 | OM lipoprotein (SEC through IM), inserted in OM by LOL), Murein hydrolase activator NlpD | Outer Membrane Lipoprotein | Proven signal peptidase II substrate. PMID: 15174130 | PMID: 15174130 PMID: 20932056 | Outer Membrane Lipoprotein | I | N/A | Cell inner membrane | Outer membrane Lipoprotein (Experimental) | SEC | LOL | yes | yes | Y | 25 | 0.134 | SpII | 25 | 21.9047 | Pos+2=S | 0 | 0 | Y | n13-24c29/30o | OuterMembrane | 9 | 85 | |||||||
| YAJG_ECOLI | P0ADA5 | yajG | b0434 JW0424 | OM lipoprotein (SEC through IM, inserted in OM by LOL), YajG | Outer Membrane Lipoprotein | Outer membrane protein determined by 2D gel electrophoresis (Lopez-Campistrous et al 2005) PMID: 9298646 | PMID: 15911532 PMID: 20932056 PMID: 9298646 | Outer Membrane Lipoprotein | I | Soluble IM Fraction | Cell membrane | Outer membrane Lipoprotein (Experimental) | SEC | LOL | yes | yes | Y | 20 | 0.403 | SpII | 17 | 19.0402 | Pos+2=A | 0 | 0 | Y | n5-15c19/20o | Unknown | 2 | ||||||||
| OSMB_ECOLI | P0ADA7 | osmB | b1283 JW1275 | OM lipoprotein (SEC through IM), inserted in OM by LOL), Osmotically-inducible B OsmB | Outer Membrane Lipoprotein | Proven signal peptidase II substrate. PMID: 15174130 | PMID: 15174130 | Outer Membrane Lipoprotein | I | N/A | Cell membrane | Outer membrane Lipoprotein (Experimental) | SEC | LOL | no | yes | Y | 24 | 0.336 | SpII | 23 | 22.8573 | Pos+2=S | 0 | 0 | Y | n9-17c22/23o | CytoplasmicMembrane | 9 | ||||||||
| OSME_ECOLI | P0ADB1 | osmE anr | anr | b1739 JW1728 | OM lipoprotein (SEC through IM), inserted in OM by LOL), Osmotically-inducible putative OsmE | Outer Membrane Lipoprotein | Proven signal peptidase II substrate. Outer membrane protein determined by 2D gel electrophoresis (Lopez-Campistrous et al 2005) Identified in nucleoids collected by sucrose gradient centrifugation and analyzed by liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS). PMID: 21541338 PMID: 10806384 Disulfide bond-containing protein PMID: 25664544 | PMID: 10806384 PMID: 15174130 PMID: 15911532 PMID: 20932056 PMID: 21541338 PMID: 25664544 | Outer Membrane Lipoprotein | I | Soluble IM Fraction | Cell inner membrane | Outer membrane Lipoprotein (Experimental) | SEC | LOL | yes | yes | Y | 24 | 0.207 | SpII | 20 | 20.0226 | Pos+2=T | 1 | 0 | Y | n7-18c23/24o | Unknown | 2 | 98 | ||||||
| ECNA_ECOLI | P0ADB4 | ecnA | b4410 JW5737 | OM lipoprotein (SEC through IM), inserted in OM by LOL), Entericidin A EcnA | Outer Membrane Lipoprotein | Small membrane protein detected experimentally. Proven pignal peptidase II substrate. PMID: 15174130 | PMID: 15174130 PMID: 19121005 | Outer Membrane Lipoprotein | I | N/A | Cell membrane; Lipid-anchor | Outer membrane Lipoprotein (Experimental) | SEC | LOL | no | yes | Y | 23 | 0.254 | SpII | 18 | 14.5257 | Pos+2=N | 0 | 0 | Y | n5-16c21/22o | CytoplasmicMembrane | 10 | ||||||||
| ECNB_ECOLI | P0ADB7 | ecnB yjeU | yjeU | b4411 JW4108 | OM lipoprotein (SEC through IM), inserted in OM by LOL), Entericidin B EcnB | Outer Membrane Lipoprotein | Potentially toxic protein. Proven pignal peptidase II substrate. Identified in nucleoids collected by sucrose gradient centrifugation and analyzed by liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS). PMID: 21541338 PMID: 15174130 | PMID: 15174130 PMID: 19121005 PMID: 20932056 PMID: 21541338 | Outer Membrane Lipoprotein | I | Soluble IM Fraction | Cell membrane; Lipid-anchor | Outer membrane Lipoprotein (Experimental) | SEC | LOL | no | yes | Y | 22 | 0.27 | SpII | 21 | 17.5085 | Pos+2=N | 1 | 0 | Y | n5-15c20/21o | CytoplasmicMembrane | 10 | |||||||
| LPTE_ECOLI | P0ADC1 | lptE rlpB | rlpB | b0641 JW0636 | OM lipoprotein (SEC through IM), inserted in OM by LOL), LPS-assembly LptE | Outer Membrane Lipoprotein | Proven signal peptidase II substrate. PMID: 16861298 | PMID: 15174130 PMID: 16861298 PMID: 20932056 PMID: 22901060 | Outer Membrane Lipoprotein | I | N/A | Cell outer membrane | Outer membrane Lipoprotein (Experimental) | SEC | LOL | yes | yes | N | 25 | 0.125 | SpII | 18 | 17.3262 | Pos+2=G | 0 | 0 | Y | n4-15c20/21o | OuterMembrane | 10 | 80 | ||||||
| TAMA_ECOLI | P0ADE4 | tamA yftM ytfM | yftM ytfM | b4220 JW4179 | OM b-barrel protein (SEC through IM, inserted in OM by BAM and TAM), Translocation and assembly module subunit TamA | Outer Membrane b-barrel protein | Part of the translocation and assembly module (TAM). TAM system promotes efficient secretion of autotransporters in proteobacteria. | PMID: 17214547 PMID: 22466966 | Outer Membrane b-barrel protein | H | N/A | Cell outer membrane | Outer Membrane ??-barrel protein | SEC | BAM | yes | yes | Y | 22 | 0.861 | SpI | 21 | 11.5811 | - | 0 | 0 | Y | n7-15c21/22o | OuterMembrane | 10 | |||||||
| YGFZ_ECOLI | P0ADE8 | ygfZ yzzW | yzzW | b2898 JW2866 | cytoplasmic & periplasmic protein (SEC through IM), tRNA-modifying YgfZ | Cytoplasmic, Periplasmic | Subcellular localization analysis of cytotoxic necrotizing factor 1 <a href="http://www.uniprot.org/uniprot/Q1R2U0">(CNF1)</a> demonstrated that YgfZ, a periplasmic protein, contributes to secretion of CNF1 (a normally cytoplasmic protein) into outer-membrane vesicles (OMVs) in pathogenic E.coli strain RS218 O18:K1:H7 PMID: 22174383. PMID: 9298646 | PMID: 22174383 PMID: 9298646 | Cytoplasmic, Periplasmic | A, G | N/A | Cytoplasm | Cytoplasmic (Experimental) | SEC | yes | yes | N | 31 | 0.252 | CYT | 0 | -0.200913 | - | 0 | 0 | N | i | Unknown | 2 | 82 | |||||||
| YIAF_ECOLI | P0ADK0 | yiaF | b3554 JW5655 | IM lipoprotein (SEC through IM, inserted in OM by LOL), YiaF | Uncharacterized protein yiaF - echolocation Occurrence of signal peptide determined by LipoP signal sequence shown based upon lipoP, not possible to determine which membrane based on +2 amino acid rule. | Inner Membrane Lipoprotein | E | Soluble IM Fraction | Membrane Lipoprotein | SEC | LOL | yes | yes | N | 28 | 0.132 | SpII | 25 | 10.3533 | Pos+2=F | 1 | 0 | Y | n10-21c26/27o | Unknown | 2 | 67 | ||||||||||
| YIDQ_ECOLI | P0ADM4 | yidQ | b3688 JW5633 | IM lipoprotein (SEC through IM, inserted in OM by LOL), YidQ | Inner Membrane Lipoprotein | PMID: 11274153 | Inner Membrane Lipoprotein | E | N/A | Outer membrane Lipoprotein(experimental) | SEC | LOL | yes | yes | Y | 27 | 0.185 | SpII | 20 | 16.396 | Pos+2=S | 0 | 0 | Y | n5-18c26/27o | Unknown | 2 | 114 | |||||||||
| YIDX_ECOLI | P0ADM6 | yidX | b3696 JW5858 | OM lipoprotein (SEC through IM, inserted in OM by LOL), YidX | Outer Membrane Lipoprotein | I | N/A | Membrane | Outer membrane Lipoprotein | SEC | LOL | pseudogene (Ochman & Davalos, 2006) | no | yes | Y | 29 | 0.411 | SpII | 23 | 20.6632 | Pos+2=I | 1 | 0 | Y | n11-21c26/27o | Unknown | 2 | 54 | |||||||||
| YIFL_ECOLI | P0ADN6 | yifL | b4558 JW3781 | OM lipoprotein (SEC through IM, inserted in OM by LOL), YifL | Outer Membrane Lipoprotein | Outer membrane protein determined by 2D gel electrophoresis (Lopez-Campistrous et al 2005) PMID: 16397293 | PMID: 15911532 PMID: 16397293 | Outer Membrane Lipoprotein | I | Soluble IM Fraction | Cell membrane | Membrane Lipoprotein (Experimental) | SEC | LOL | no | yes | Y | 24 | 0.159 | SpII | 19 | 27.2584 | Pos+2=G | 1 | 0 | Y | n7-18c23/24o | Unknown | 2 | ||||||||
| YGGE_ECOLI | P0ADS6 | yggE | b2922 JW2889 | periplasmic protein (SEC through IM), YggE | Periplasmic | G | N/A | Periplasmic | SEC | yes | yes | Y | 22 | 0.409 | SpI | 21 | 16.0009 | - | 0 | 0 | Y | n5-16c21/22o | Periplasmic | 9 | 111 | ||||||||||||
| YGGN_ECOLI | P0ADS9 | yggN | b2958 JW2925 | periplasmic protein (SEC through IM), YggN | Periplasmic | Substrate of periplasmic disulfide oxidoreductases DsbA. Identified DsbA substrate PMID: 19953543 PMID: 14726535 | PMID: 14726535 PMID: 19953543 | Periplasmic | G | N/A | Periplasmic(experimental) | SEC | DSBA | yes | yes | Y | 21 | 0.797 | SpI | 20 | 21.2471 | - | 0 | 0 | Y | n6-16c20/21o | Unknown | 2 | 80 | ||||||||
| YGIW_ECOLI | P0ADU5 | ygiW | b3024 JW2992 | periplasmic protein (SEC through IM), YgiW | Periplasmic | SecB substrate. PMID: 9298646 | PMID: 16352602 PMID: 9298646 | Periplasmic | G | N/A | Periplasm | Periplasmic (Experimental) | SECB | SEC | yes | yes | Y | 21 | 0.877 | SpI | 20 | 26.1756 | - | 0 | 0 | Y | n4-15c20/21o | Unknown | 2 | 28 | |||||||
| LPTA_ECOLI | P0ADV1 | lptA yhbN | yhbN | b3200 JW3167 | periplasmic protein (SEC through IM), polysaccharide export system LptA | Periplasmic | PMID: 17056748 PMID: 18480051 PMID: 9298646 | Periplasmic | G | N/A | Periplasm | Periplasmic(experimental) | SEC | yes | yes | Y | 28 | 0.838 | SpI | 27 | 24.4302 | - | 0 | 0 | Y | n12-23c27/28o | Unknown | 2 | 23 | ||||||||
| MLAC_ECOLI | P0ADV7 | mlaC yrbC | yrbC | b3192 JW3159 | periplasmic protein (SEC through IM), Interphospholipid transport system binding MlaC | Periplasmic | PMID: 15911532 | Periplasmic | G | N/A | Periplasm | Periplasmic (Experimental) | SEC | no | yes | Y | 22 | 0.509 | SpI | 21 | 14.0896 | - | 0 | 0 | Y | n8-16c21/22o | Unknown | 2 | 61 | ||||||||
| YHHA_ECOLI | P0ADX7 | yhhA | b3448 JW3413 | periplasmic protein (SEC through IM), YhhA | Periplasmic | G | N/A | Periplasmic | SEC | yes | yes | Y | 18 | 0.377 | SpI | 17 | 14.5283 | - | 0 | 0 | Y | n4-11c17/18o | Unknown | 2 | 107 | ||||||||||||
| ACRA_ECOLI | P0AE06 | acrA lir mtcA | lir mtcA | b0463 JW0452 | IM lipoprotein (SEC through IM), inserted in OM by LOL), Multidrug efflux pump subunit AcrA | Inner Membrane Lipoprotein | Proven signal peptidase II substrate. PMID: 10894736 | PMID: 10894736 PMID: 15174130 PMID: 15228545 PMID: 16079137 | Inner Membrane Lipoprotein | E | Soluble IM Fraction | Cell inner membrane | Inner membrane lipoprotein (Experimental) | SEC | LOL | yes | yes | Y | 22 | 0.194 | SpII | 24 | 19.636 | Pos+2=D | 0 | 0 | Y | n6-16c21/22o | CytoplasmicMembrane | 10 | 64 | ||||||
| APHA_ECOLI | P0AE22 | aphA napA yjbP | napA yjbP | b4055 JW4015 | periplasmic protein (SEC through IM), Class B acid phosphatase AphA | Periplasmic | PMID: 9011040 PMID: 9298646 | Periplasmic | G | N/A | Periplasm | Periplasmic (Experimental) | SEC | no | yes | Y | 26 | 0.643 | SpI | 25 | 14.5914 | - | 0 | 0 | Y | n7-17c25/26o | Unknown | 2 | |||||||||
| CPXP_ECOLI | P0AE85 | cpxP yiiO | yiiO | b4484 JW5558 | periplasmic peripheral IM protein (SEC through IM), CpxP | Peripheral inner membrane protein facing the periplasm | PMID: 10972835 PMID: 17904518 PMID: 21317318 | Peripheral inner membrane protein facing the periplasm | F2 | N/A | Periplasm | Membrane associated (Experimental) | SEC | pseudogene (Ochman & Davalos, 2006) | yes | yes | Y | 22 | 0.806 | SpI | 21 | 16.0374 | - | 0 | 0 | Y | n3-14c21/22o | Periplasmic | 10 | 104 | |||||||
| CREA_ECOLI | P0AE91 | creA yjjD | yjjD | b4397 JW4360 | periplasmic protein (SEC through IM), CreA | Periplasmic | Identified DsbA substrate PMID: 19953543 Disulfide bond-containing protein PMID: 25664544 | PMID: 19953543 PMID: 25664544 | Periplasmic | G | N/A | Periplasmic | SEC | DSBA | yes | yes | Y | 22 | 0.8 | SpI | 21 | 14.9398 | - | 0 | 0 | Y | n6-16c21/22o | Unknown | 2 | ||||||||
| CSGE_ECOLI | P0AE95 | csgE | b1039 JW1022 | Curli related periplasmic protein (SEC through IM), Curli production assembly/transport component CsgE | Periplasmic | Potential substrate of periplasmic peptidyl prolyl cis???trans isomerase SurA; sequence contains known binding motif of SurA [RYEF]X[RYEF][P]. Curli secretion specificity factor. CsgE provides substrate specificity to the curli secretion pore CsgG, and acts directly on the secretion substrate CsgA to prevent premature subunit assembly. PMID: 9928475 | PMID: 16704339 PMID: 18498364 PMID: 19131513 PMID: 21645131 PMID: 9928475 | Periplasmic | G | N/A | Periplasmic (Experimental) | Curli | SEC | SURA | yes | yes | Y | 23 | 0.789 | SpI | 22 | 3.89784 | - | 0 | 0 | Y | n7-17c22/23o | Unknown | 2 | 15 | |||||||
| CSGF_ECOLI | P0AE98 | csgF | b1038 JW1021 | Curli related extra-cellular peripheral OM protein (SEC through IM), secreted over OM by curli), Curli production assembly/transport component CsgF | Peripheral outer membrane protein facing the extra-cellular space | Curli component. CsgF is secreted to the cell surface, where it mediates the cell-association and protease-resistance of the CsgB nucleator, suggesting that CsgF is required for specific localization and/or chaperoning of CsgB for full nucleator activity. Curli are a functional amyloid whose in vivo polymerization requires a dedicated nucleator protein, CsgB, and an assembly protein, CsgF. Without CsgF, curli subunits are released from the cell into the media and are inefficiently polymerized, resulting in fewer and mislocalized curli fibers. PMID: 19131513 PMID: 9928475 | PMID: 16522795 PMID: 16704339 PMID: 19131513 PMID: 21645131 PMID: 9928475 | Peripheral outer membrane protein facing the extra-cellular space | F4 | N/A | Cell outer membrane | Periplasmic (Experimental) | Curli | SEC | Curli | yes | yes | Y | 20 | 0.853 | SpI | 19 | 13.9164 | - | 1 | 0 | Y | n6-14c19/20o | Extracellular | 9 | 30 | ||||||
| CSGG_ECOLI | P0AEA2 | csgG | b1037 JW1020 | Curli related OM lipoprotein & extra-cellular peripheral OM protein (SEC through IM), inserted in OM by LOL), Curli production assembly/transport component CsgG | Outer Membrane Lipoprotein, Peripheral outer membrane protein facing the extra-cellular space | Curli component. Proven signal peptidase II substrate. CsgG is an outer membrane protein required for the secretion of the two curli structural subunits CsgA and CsgB. CsgG is a lipoprotein that spans the outermembrane and assembles into an oligomeric annular-shaped structure with an inner diameter of approximately 2 nm (Margery L. Evans and Matthew R. Chapman in press). Overexpression of CsgG results in increased sensitivity to the small antibiotic erythromycin (740Da) but not the larger vancomycin (1440Da) suggesting that CsgG has a pore-like activity (Robinson et al 2006). PMID: 15174130 | PMID: 15174130 PMID: 16420357 PMID: 16704339 PMID: 19131513 PMID: 21645131 | Outer Membrane Lipoprotein, Peripheral outer membrane protein facing the extra-cellular space | I, F4 | N/A | Cell membrane | Outer membrane Lipoprotein (Experimental) | Curli | SEC | LOL | yes | yes | Y | 20 | 0.544 | SpII | 15 | 12.9163 | Pos+2=L | 0 | 0 | Y | n4-13c18/19o | CytoplasmicMembrane | 9 | 44 | ||||||
| DACA_ECOLI | P0AEB2 | dacA pfv | pfv | b0632 JW0627 | periplasmic peripheral IM protein (SEC through IM), D-alanyl-D-alanine carboxypeptidase DacA | Peripheral inner membrane protein facing the periplasm | Anchored to inner membrane by C-terminal tailPMID: 2194801. Using natural isotope abundance NMR, the structure of the PBP 5 anchor peptide within a micelle was determinedPMID: 20192190. The structure conforms to a helix-bend-helix-turn-helix motif and reveals that the anchor enters the membrane so as to form an amphiphilic structure within the interface of the hydrophilic/hydrophobic boundary regions near the lipid head groups. PBP-5 deletion mutants exhibit irregular distribution of MreB at the deformed zones PMID: 20545860. PMID: 3279397 PMID: 8424800 DacA(PBP-5) anchors to the periplasmic face of the inner membrane via C-terminal amphiphilic alpha-helix. The Alpha-helical conformation in the C-terminal anchoring domain is observed in aqueous solution or in the presence of phospholipid vesicles. A pH dependent loss of alpha-helical conformation in the peptide corresponding to the PBP5 C-terminus was found to correlate with the susceptibility of PBP5 to membrane extraction. PMID: 9371419 | PMID: 20192190 PMID: 20545860 PMID: 2194801 PMID: 24057063 PMID: 3279397 PMID: 8424800 PMID: 9371419 | Peripheral inner membrane protein facing the periplasm | F2 | Soluble IM Fraction | Cell inner membrane; Peripheral membrane protein; Cytoplasmic side | Membrane anchored (Experimental) | SEC | yes | yes | Y | 30 | 0.737 | SpI | 29 | 17.6151 | - | 0 | 0 | Y | n13-24c29/30o | CytoplasmicMembrane | 9 | 8 | |||||||
| DCRB_ECOLI | P0AEE1 | dcrB yhhR | yhhR | b3472 JW5682 | IM lipoprotein (SEC through IM, inserted in OM by LOL), DcrB | Inner Membrane Lipoprotein | PMID: 12558182 PMID: 15911532 | Inner Membrane Lipoprotein | E | Soluble IM Fraction | Periplasm | Inner membrane lipoprotein | SEC | LOL | yes | yes | N | 30 | 0.169 | SpII | 19 | 21.1 | Pos+2=D | 0 | 0 | Y | n8-15c20/21o | Periplasmic | 10 | 100 | |||||||
| DEGS_ECOLI | P0AEE3 | degS hhoB htrH | hhoB htrH | b3235 JW3204 | periplasmic protein (SEC through IM), Serine endoprotease DegS | Periplasmic | Proteolysis of transmembrane and cytoplasmic portions of RseA by DegS frees the sigma(E) transcription factor. RseB protein, a known negative regulator, inhibits proteolysis by DegS in vitro by binding tightly to the periplasmic domain of RseA. PMID: 17360428 | PMID: 11442831 PMID: 17360428 | Periplasmic | G | Soluble IM Fraction | Cell inner membrane | Membrane anchored | SEC | yes | yes | N | 28 | 0.167 | SpI | 28 | 4.49468 | - | 1 | 0 | Y | n9-20c28/29o | Periplasmic | 9 | 75 | |||||||
| DGAL_ECOLI | P0AEE5 | mglB GBP | GBP | b2150 JW2137 | periplasmic protein (SEC through IM), D-galactose-binding periplasmic MglB | Periplasmic | Potential Skp substrate PMID: 19953543 PMID: 7012152 PMID: 9298646 | PMID: 19953543 PMID: 7012152 PMID: 9298646 | Periplasmic | G | N/A | Periplasm | Periplasmic (Experimental) | SEC | SKP | no | yes | Y | 24 | 0.861 | SpI | 23 | 23.7963 | - | 1 | 0 | Y | n5-16c23/24o | Periplasmic | 10 | 75 | ||||||
| DSBA_ECOLI | P0AEG4 | dsbA dsf ppfA | dsf ppfA | b3860 JW3832 | periplasmic protein (SEC through IM), Thiol:disulfide interchange DsbA | Periplasmic | Periplasmic disulfide isomerase DsbC cooperates with SurA and the thiol oxidase DsbA in the folding of the essential ??-barrel protein LptD. DsbA is co-translationally translocated through Sec translocon. PMID: 9298646 DsbA, is a substrate for the SRP pathway. PMID: 15838024 DsbA, is a substrate for the SRP pathway. PMID: 13129941 Disulfide bridge (49 52) PMID: 9300489 Disulfide bond-containing protein PMID: 25664544 | PMID: 13129941 PMID: 15838024 PMID: 18406599 PMID: 19525348 PMID: 20615876 PMID: 25664544 PMID: 9298646 PMID: 9300489 | Periplasmic | G | N/A | Periplasm | Periplasmic (Experimental) | SRP (exp) | SEC | yes | yes | Y | 20 | 0.75 | SpI | 19 | 16.7987 | - | 0 | 0 | Y | n4-15c19/20o | Periplasmic | 10 | 71 | ||||||
| DSBC_ECOLI | P0AEG6 | dsbC xprA | xprA | b2893 JW2861 | periplasmic protein (SEC through IM), Thiol:disulfide interchange DsbC | Periplasmic | Periplasmic disulfide isomerase DsbC cooperates with SurA and the thiol oxidase DsbA in the folding of the essential ??-barrel protein LptD. PMID: 9298646 Disulfide bridge (118 121;161 183) PMID: 10700276 Disulfide bond-containing protein PMID: 25664544 | PMID: 10700276 PMID: 18406599 PMID: 20615876 PMID: 25664544 PMID: 8168498 PMID: 9298646 | Periplasmic | G | N/A | Periplasm | Periplasmic (Experimental) | SEC | yes | yes | Y | 21 | 0.778 | SpI | 20 | 21.5074 | - | 0 | 0 | Y | n4-15c20/21o | Periplasmic | 10 | 82 | |||||||
| FEPB_ECOLI | P0AEL6 | fepB | b0592 JW0584 | periplasmic protein (SEC through IM), Ferrienterobactin-binding periplasmic protein FepB | Periplasmic | PMID: 10986237 | Periplasmic | G | N/A | Periplasm | Periplasmic (Experimental) | SEC | yes | yes | Y | 27 | 0.581 | SpI | 26 | 13.3736 | - | 0 | 0 | Y | n10-21c26/27o | Periplasmic | 10 | 30 | |||||||||
| FLGM_ECOLI | P0AEM4 | flgM | b1071 JW1058 | Flagellar cytoplasmic & extra-cellular protein (Flagellum secreted), Negative regulator of flagellin synthesis FlgM | Cytoplasmic, Extra-cellular | Anti-sigma28 factor. FlgM is an anti-sigma factor that binds and inactivates sigma28 (FliA_Ecoli). Once the hook and basal body is assembled, FlgM is secreted by the flagellar export system thereby freeing sigma28 to activate transcription. | PMID: 10322161 PMID: 12631703 PMID: 9988479 | Cytoplasmic, Extra-cellular | A, X | N/A | Cytoplasmic | Flagellum | Fla | yes | yes | N | 49 | 0.115 | CYT | 0 | -0.200913 | - | 0 | 0 | N | i | Cytoplasmic | 9 | 89 | ||||||||
| FLIY_ECOLI | P0AEM9 | fliY yzzR | yzzR | b1920 JW1905 | periplasmic protein (SEC through IM), L-cystine-binding FliY | Periplasmic | PMID: 9298646 | Periplasmic | G | N/A | Periplasm | Periplasmic (Experimental) | SEC | no | yes | N | 30 | 0.555 | SpI | 29 | 10.7798 | - | 0 | 0 | Y | n10-21c29/30o | Periplasmic | 10 | 96 | ||||||||
| FTSL_ECOLI | P0AEN4 | ftsL mraR yabD | mraR yabD | b0083 JW0081 | IM lipoprotein (SEC through IM), Cell division FtsL | Inner Membrane Lipoprotein | Localizes to potential division sites in filamentous cells. Proven pignal peptidase II substrate. PMID: 1332942 | PMID: 10027987 PMID: 11703663 PMID: 11948172 PMID: 1332942 PMID: 15165235 PMID: 15174130 PMID: 21890895 | Inner Membrane Lipoprotein | E | IM (predicted) | Cell inner membrane | Membrane anchored (Experimental) | SEC | yes | yes | N | 24 | 0.167 | CYT | 0 | -0.200913 | - | 1 | 1 | N | o35-57i | Cytoplasmic | 8 | 25 | |||||||
| GLNH_ECOLI | P0AEQ3 | glnH | b0811 JW0796 | periplasmic protein (SEC through IM), Glutamine-binding periplasmic GlnH | Periplasmic | Potential substrate of periplasmic peptidyl prolyl cis???trans isomerase SurA; sequence contains known binding motif of SurA [RYEF]X[RYEF][P]. PMID: 9298646 Potential substrate for the SRP pathway. It was found to be down-regulated in the periplasm and up-regulated in the cytoplasm of SRP-depleted cells. PMID: 22030350 | PMID: 18498364 PMID: 22030350 PMID: 9298646 | Periplasmic | G | N/A | Periplasm | Periplasmic (Experimental) | SEC | SURA | yes | yes | Y | 23 | 0.851 | SpI | 22 | 19.9168 | - | 0 | 0 | Y | n7-18c22/23o | Periplasmic | 10 | 62 | |||||||
| HDEA_ECOLI | P0AES9 | hdeA yhhC yhiB | yhhC yhiB | b3510 JW3478 | periplasmic protein (SEC through IM), Acid stress chaperone HdeA | Periplasmic | Experimentally verified gene-product. Verified that the signal peptide is cleaved. Falls into an unstructured form that is essential for its chaperone activity. The protein is dimeric at neutral pH but dissociates into a partially unfolded monomer at pH 2.0 PMID: 20080625. PMID: 8455549 Potential substrate of periplasmic chaperone YfgM. PMID: 25403562 Putative substrate for the periplasmic chaperone YfgM. PMID: 25403562 Disulfide bridge (39 87) Disulfide bond-containing protein PMID: 25664544 | PMID: 10623550 PMID: 17085547 PMID: 20080625 PMID: 25403562 PMID: 25664544 PMID: 8455549 PMID: 9868784 | Periplasmic | G | N/A | Periplasm | Periplasmic (Experimental) | SEC | YfgM | yes | yes | Y | 22 | 0.698 | SpI | 21 | 16.385 | - | 1 | 0 | Y | n4-15c21/22o | Unknown | 2 | 108 | ||||||
| HDEB_ECOLI | P0AET2 | hdeB yhhD yhiC | yhhD yhiC | b3509 JW5669 | periplasmic protein (SEC through IM), Acid stress chaperone HdeB | Periplasmic | Experimentally verified gene-product. Verified that the signal peptide is cleaved. PMID: 8455550 Potential substrate of periplasmic chaperone YfgM. PMID: 25403562 Putative substrate for the periplasmic chaperone YfgM. PMID: 25403562 Disulfide bond-containing protein PMID: 25664544 | PMID: 17085547 PMID: 25403562 PMID: 25664544 PMID: 8455550 PMID: 9868784 | Periplasmic | G | N/A | Periplasm | Periplasmic (Experimental) | SEC | YfgM | yes | yes | Y | 30 | 0.837 | SpI | 29 | 16.8932 | - | 0 | 0 | Y | n10-21c29/30o | Unknown | 2 | 63 | ||||||
| HISJ_ECOLI | P0AEU0 | hisJ | b2309 JW2306 | periplasmic protein (SEC through IM), Histidine-binding periplasmic HisJ | Periplasmic | Substrate of periplasmic disulfide oxidoreductases DsbA. Identified DsbA substrate PMID: 19953543 PMID: 9298646 Disulfide bridge (60 67) Disulfide bond-containing protein PMID: 25664544 | PMID: 14726535 PMID: 19953543 PMID: 25664544 PMID: 9298646 | Periplasmic | G | N/A | Periplasm | Periplasmic (Experimental) | SEC | DSBA | yes | yes | Y | 23 | 0.792 | SpI | 22 | 20.7158 | - | 1 | 0 | Y | n4-14c22/23o | Periplasmic | 10 | ||||||||
| SKP_ECOLI | P0AEU7 | skp hlpA ompH | hlpA ompH | b0178 JW0173 | periplasmic protein (SEC through IM), Chaperone Skp | Periplasmic | Folding of OmpA from its complex with the periplasmic chaperone Skp into bilayers composed of phosphatidylethanolamine and phosphatidylcholine was inhibited in the absence of BamA but facilitated when BamA was present, indicating an interaction of Skp-OmpA complexes with BamA. PMID: 2167239 PMID: 9298646 Potential substrate for the SRP pathway. It was found to be down-regulated in the periplasm and up-regulated in the cytoplasm of SRP-depleted cells. PMID: 22030350 | PMID: 1838129 PMID: 19525348 PMID: 21370981 PMID: 2167239 PMID: 22030350 PMID: 2318304 PMID: 23641708 PMID: 8730870 PMID: 9298646 | Periplasmic | G | N/A | Periplasm | Periplasmic(experimental) | SEC | yes | yes | Y | 21 | 0.881 | SpI | 20 | 22.0906 | - | 0 | 0 | Y | n4-15c20/21o | Periplasmic | 10 | 48 | |||||||
| MALE_ECOLI | P0AEX9 | malE | b4034 JW3994 | periplasmic & peripheral IM protein (SEC through IM), Maltose/maltodextrin-binding periplasmic MalE | Peripheral inner membrane protein facing the periplasm, periplasmic | Potential substrate of periplasmic peptidyl prolyl cis???trans isomerase SurA. Sequence contains known binding motif of SurA [RYEF]X[RYEF][P]. MalE is maintained in an unfolded conformation by the cytoplasmic chaperone SecB. Potential Skp substrate PMID: 19953543 PMID: 9298646 An amber mutation in the secA gene of Escherichia coli causes a pleiotropic decrease in the synthesis of secreted proteins. PMID: 3009404 Potential substrate of periplasmic chaperone YfgM. PMID: 25403562 Putative substrate for the periplasmic chaperone YfgM. PMID: 25403562 | PMID: 18498364 PMID: 1989077 PMID: 19953543 PMID: 25403562 PMID: 3009404 PMID: 9298646 | Peripheral inner membrane protein facing the periplasm, periplasmic | F2, G | N/A | Periplasm | Periplasmic (Experimental) | SecB | SEC | SURA,SKP,YfgM | yes | yes | Y | 27 | 0.712 | SpI | 26 | 17.3972 | - | 0 | 0 | Y | n9-17c26/27o | Periplasmic | 10 | 96 | ||||||
| MLTD_ECOLI | P0AEZ7 | mltD dniR yafG | dniR yafG | b0211 JW5018 | OM lipoprotein (SEC through IM), inserted in OM by LOL), Membrane-bound lytic murein transglycosylase D MltD | Outer Membrane Lipoprotein | Proven signal peptidase II substrate. PMID: 15174133 | PMID: 15174130 PMID: 15174133 | Outer Membrane Lipoprotein | I | N/A | Cell membrane | Outer membrane Lipoprotein (Experimental) | SEC | LOL | yes | yes | N | 27 | 0.22 | SpII | 15 | 18.5563 | Pos+2=Q | 0 | 0 | Y | n5-13c18/19o | Unknown | 2 | 29 | ||||||
| YJBE_ECOLI | P0AF45 | yjbE | b4026 JW3986 | periplasmic protein (SEC through IM), YjbE | Periplasmic | G | N/A | Periplasmic | SEC | no | yes | Y | 22 | 0.853 | SpI | 21 | 25.8362 | - | 0 | 0 | Y | n4-15c21/22o | Extracellular | 9 | |||||||||||||
| YJCO_ECOLI | P0AF56 | yjcO | b4078 JW4039 | periplasmic protein (SEC through IM), Sel1-repeat-containing YjcO | Periplasmic | Disulfide bond-containing protein PMID: 25664544 | PMID: 25664544 | Periplasmic | G | N/A | Periplasmic | SEC | yes | yes | Y | 18 | 0.752 | SpI | 17 | 13.9637 | - | 0 | 0 | Y | n4-13c17/18o | Unknown | 2 | 82 | |||||||||
| YJEI_ECOLI | P0AF70 | yjeI | b4144 JW5736 | OM lipoprotein (SEC through IM, inserted in OM by LOL), YjeI | Outer Membrane Lipoprotein | Proven pignal peptidase II substrate. Outer membrane protein determined by LC-MS (Gevaert et al 2002) PMID: 15174130 | PMID: 12488465 PMID: 15174130 PMID: 20932056 | Outer Membrane Lipoprotein | I | Soluble IM Fraction | Outer membrane Lipoprotein (Experimental) | SEC | LOL | yes | yes | Y | 28 | 0.443 | SpII | 18 | 20.5638 | Pos+2=S | 0 | 0 | Y | n6-17c22/23o | Unknown | 2 | 87 | ||||||||
| YJFN_ECOLI | P0AF82 | yjfN | b4188 JW5742 | periplasmic protein (SEC through IM), YjfN | Periplasmic | G | N/A | Periplasm | Periplasmic | SEC | yes | yes | N | 20 | 0.293 | SpI | 21 | 7.51366 | - | 0 | 0 | Y | n4-15c23/24o | Unknown | 2 | 104 | |||||||||||
| YJFY_ECOLI | P0AF86 | yjfY | b4199 JW4157 | periplasmic protein (SEC through IM), YjfY | Periplasmic | G | N/A | Periplasm | Periplasmic | SEC | yes | yes | Y | 21 | 0.833 | SpI | 20 | 15.6931 | - | 0 | 0 | Y | n5-15c20/21o | Unknown | 2 | 93 | |||||||||||
| NLPI_ECOLI | P0AFB1 | nlpI yhbM | yhbM | b3163 JW3132 | OM lipoprotein (SEC through IM, inserted in OM by LOL), NlpI | Outer Membrane Lipoprotein | Proven pignal peptidase II substrate. PMID: 15174130 Induction of NlpI inhibits nucleoid division and halts cell growth. Elevation of NlpI expression enhances the transcription and the outer membrane localization of the heat shock protein IbpA and IbpB. NlpI physically interacts with IbpB. The C-terminus of NlpI is critical for the enhancement in IbpA and IbpB production, and the N-terminus of NlpI is required for the outer membrane localization of NlpI, IbpA, and IbpB. PMID: 25699035 | PMID: 15174130 PMID: 25699035 | Outer Membrane Lipoprotein | I | N/A | Cell membrane; Lipid-anchor | Outer membrane Lipoprotein (Experimental) | SEC | LOL | yes | yes | N | 26 | 0.209 | SpII | 18 | 14.4676 | Pos+2=S | 0 | 0 | Y | n8-19c31/32o | CytoplasmicMembrane | 10 | 56 | ||||||
| OSMY_ECOLI | P0AFH8 | osmY | b4376 JW4338 | periplasmic & extra-cellular protein (SEC through IM), Osmotically-inducible Y OsmY | Periplasmic, Extra-cellular | The most efficient excreting fusion partner, OsmY, was used as a carrier protein to excrete heterologous proteins into the medium. PMID: 18727129 The OsmY protein is an Escherichia coli 20 kDa outer membrane or periplasmic protein that is expressed in response to a variety of stress conditions, in particular, helping to provide protection against osmotic shock. One hypothesis is that OsmY prevents shrinkage of the cytoplasmic compartment by contacting the phospholipid interfaces surrounding the periplasmic space. The domain architecture of two BON domains alone suggests that these domains contact the surfaces of phospholipids, with each domain contacting a membrane PMID: 12175925 | PMID: 12878000 PMID: 1317380 PMID: 18727129 PMID: 9298646 | Periplasmic, Extra-cellular | G, X | N/A | Periplasm | Periplasmic (Experimental) | SEC | yes | yes | Y | 29 | 0.787 | SpI | 28 | 23.4143 | - | 0 | 0 | Y | n12-23c28/29o | Periplasmic | 10 | 108 | ||||||||
| PBP7_ECOLI | P0AFI5 | pbpG yohB | yohB | b2134 JW5355 | periplasmic protein (SEC through IM), D-alanyl-D-alanine endopeptidase PbpG | Periplasmic | PMID: 7721700 | Periplasmic | G | N/A | Periplasm | Periplasmic (Experimental) | SEC | yes | yes | Y | 26 | 0.58 | SpI | 25 | 17.1021 | - | 0 | 0 | Y | n6-17c25/26o | Periplasmic | 10 | 82 | ||||||||
| POTD_ECOLI | P0AFK9 | potD | b1123 JW1109 | periplasmic protein (SEC through IM), Spermidine/putrescine-binding periplasmic PotD | Periplasmic | Potential substrate for the SRP pathway. It was found to be down-regulated in the periplasmic compartment of SRP-depleted cells. PMID: 22030350 | PMID: 1939142 PMID: 22030350 PMID: 9298646 | Periplasmic | G | N/A | Periplasm | Periplasmic (Experimental) | SEC | yes | yes | Y | 24 | 0.865 | SpI | 23 | 25.0348 | - | 0 | 0 | Y | n8-18c23/24o | Periplasmic | 10 | |||||||||
| PPIA_ECOLI | P0AFL3 | ppiA rot rotA | rot rotA | b3363 JW3326 | periplasmic protein (SEC through IM), Peptidyl-prolyl cis-trans isomerase A PpiA | Periplasmic | Potential substrate for the SRP pathway. It was found to be down-regulated in the periplasmic compartment of SRP-depleted cells. PMID: 22030350 | PMID: 2190212 PMID: 22030350 | Periplasmic | G | N/A | Periplasm | Periplasmic (Experimental) | SEC | yes | yes | Y | 25 | 0.753 | SpI | 24 | 24.1414 | - | 0 | 0 | Y | n4-15c24/25o | Periplasmic | 10 | 73 | |||||||
| PROX_ECOLI | P0AFM2 | proX proU | proU | b2679 JW2654 | periplasmic protein (SEC through IM), Glycine betaine/proline betaine-binding periplasmic ProX | Periplasmic | Potential substrate of periplasmic peptidyl prolyl cis???trans isomerase SurA; sequence contains known binding motif of SurA [RYEF]X[RYEF][P]. Identified DsbA substrate PMID: 19953543 PMID: 3305496 Disulfide bridge (157 163) Disulfide bond-containing protein PMID: 25664544 | PMID: 18498364 PMID: 19953543 PMID: 25664544 PMID: 3305496 | Periplasmic | G | N/A | Periplasm | Periplasmic (Experimental) | SEC | SURA,DSBA | yes | yes | Y | 22 | 0.863 | SpI | 21 | 16.9579 | - | 0 | 0 | Y | n5-16c21/22o | Periplasmic | 10 | 52 | ||||||
| PSIF_ECOLI | P0AFM4 | psiF | b0384 JW5054 | periplasmic protein (SEC through IM), Phosphate starvation-inducible PsiF | Periplasmic | Disulfide bond-containing protein PMID: 25664544 | PMID: 25664544 | Periplasmic | G | N/A | Periplasmic | SEC | yes | yes | Y | 22 | 0.376 | SpI | 21 | 13.709 | - | 1 | 0 | Y | n5-16c21/22o | Unknown | 2 | 44 | |||||||||
| MEPS_ECOLI | P0AFV4 | mepS spr yeiV | spr yeiV | b2175 JW2163 | OM lipoprotein (SEC through IM), inserted in OM by LOL), Murein DD-endopeptidase MepS/Murein LD-carboxypeptidase MepS | Outer Membrane Lipoprotein | Proven pignal peptidase II substrate. PMID: 15174130 | PMID: 15174130 | Outer Membrane Lipoprotein | I | N/A | Cell outer membrane | Outer membrane Lipoprotein (Experimental) | SEC | LOL | yes | yes | Y | 34 | 0.288 | SpII | 26 | 11.4182 | Pos+2=S | 0 | 0 | Y | n14-24c29/30o | Unknown | 2 | 36 | ||||||
| RSEB_ECOLI | P0AFX9 | rseB | b2571 JW2555 | periplasmic protein (SEC through IM), Sigma-E factor regulatory RseB | Periplasmic | Responce to stress complex RpoE-RseA-RseB. Proteolysis of transmembrane and cytoplasmic portions of RseA by DegS frees the sigma(E) transcription factor. RseB protein, a known negative regulator, inhibits proteolysis by DegS in vitro by binding tightly to the periplasmic domain of RseA. PMID: 17360428 The N-terminal domain of RseA sequesters ??E in the cytoplasmic membrane, preventing its association with core RNA polymerase. The C-terminal domain of RseA interacts with RseB, a periplasmic protein. PMID: 11777003 PMID: 11777003 | PMID: 11777003 PMID: 17360428 PMID: 9159522 PMID: 9159523 | Periplasmic | G | N/A | Periplasm | Periplasmic (Experimental) | SEC | yes | yes | Y | 24 | 0.61 | SpI | 23 | 10.5992 | - | 0 | 0 | Y | n4-16c28/29o | Periplasmic | 9 | 15 | ||||||||
| SUBI_ECOLI | P0AG78 | sbp | b3917 JW3888 | periplasmic protein (SEC through IM), Sulfate-binding Sbp | Periplasmic | Potential substrate of periplasmic peptidyl prolyl cis???trans isomerase SurA; sequence contains known binding motif of SurA [RYEF]X[RYEF][P]. PMID: 9298646 | PMID: 18498364 PMID: 9298646 | Periplasmic | G | N/A | Periplasm | Periplasmic (Experimental) | SEC | SURA | yes | yes | Y | 20 | 0.802 | SpI | 19 | 15.2757 | - | 0 | 0 | Y | n4-14c19/20o | Periplasmic | 10 | 70 | |||||||
| UGPB_ECOLI | P0AG80 | ugpB | b3453 JW3418 | periplasmic protein (SEC through IM), sn-glycerol-3-phosphate-binding periplasmic UgpB | Periplasmic | Identified DsbA substrate PMID: 19953543 PMID: 3062310 Disulfide bond-containing protein PMID: 25664544 | PMID: 19953543 PMID: 25664544 PMID: 3062310 | Periplasmic | G | N/A | Periplasm | Periplasmic (Experimental) | SEC | DSBA | yes | yes | Y | 24 | 0.804 | SpI | 23 | 17.1867 | - | 0 | 0 | Y | n7-18c23/24o | Periplasmic | 10 | 64 | |||||||
| PSTS_ECOLI | P0AG82 | pstS phoS | phoS | b3728 JW3706 | periplasmic protein (SEC through IM), Phosphate-binding PstS | Periplasmic | Potential substrate of periplasmic peptidyl prolyl cis???trans isomerase SurA; sequence contains known binding motif of SurA [RYEF]X[RYEF][P]. Copurifies with trigger factor in pull down assays. PMID: 9298646 | PMID: 18498364 PMID: 19737520 PMID: 9298646 | Periplasmic | G | N/A | Periplasm | Periplasmic (Experimental) | TF | SEC | SURA | yes | yes | Y | 26 | 0.546 | SpI | 25 | 23.9055 | - | 1 | 0 | Y | n6-17c25/26o | Periplasmic | 10 | 79 | |||||
| SLT_ECOLI | P0AGC3 | slt sltY | sltY | b4392 JW4355 | periplasmic protein (SEC through IM), Soluble lytic murein transglycosylase Slt | Periplasmic | Disulfide bridge (133 166) Disulfide bond-containing protein PMID: 25664544 | PMID: 1938883 PMID: 25664544 | Periplasmic | G | N/A | Periplasm | Periplasmic (Experimental) | SEC | yes | yes | Y | 28 | 0.803 | SpI | 27 | 12.0915 | - | 0 | 0 | Y | n11-22c27/28o | Periplasmic | 10 | 10 | |||||||
| MLTF_ECOLI | P0AGC5 | mltF yfhD | yfhD | b2558 JW2542 | periplasmic peripheral OM protein (SEC through IM), Membrane-bound lytic murein transglycosylase F MltF | Peripheral outer membrane protein facing the periplasm | PMID: 18234673 | Peripheral outer membrane protein facing the periplasm | F3 | N/A | Cell outer membrane | Periplasmic | SEC | no | yes | N | 33 | 0.227 | SpI | 30 | 3.07095 | - | 1 | 0 | Y | n9-20c32/33o | Cytoplasmic | 9 | 10 | ||||||||
| SODC_ECOLI | P0AGD1 | sodC | b1646 JW1638 | periplasmic protein (SEC through IM), Superoxide dismutase SodC | Periplasmic | Disulfide bridge (74 169) PMID: 9405149 Disulfide bond-containing protein PMID: 25664544 | PMID: 25664544 PMID: 8626323 PMID: 9405149 | Periplasmic | G | N/A | Periplasm | Periplasmic (Experimental) | SEC | yes | yes | Y | 20 | 0.82 | SpI | 19 | 16.2903 | - | 0 | 0 | Y | n4-14c19/20o | Periplasmic | 10 | |||||||||
| SODF_ECOLI | P0AGD3 | sodB | b1656 JW1648 | periplasmic protein (SEC through IM), Superoxide dismutase SodB | Periplasmic | The superoxide dismutase SodA is targeted to the periplasm in a SecA-dependent manner by a novel mechanism in Rhizobium leguminosarum. SodM and SodF are <i>E.coli</i> proteins that are close homoloques of the RISodA. SodA does not possess a hydrophobic cleaved N-terminal signal peptide typically present in soluble proteins exported by the Sec-dependent (Sec) pathway or the twin-arginine translocation (TAT) pathway. TatC or SecB mutants in Rhizobium leguminosarum do not affect export of SodA to the periplasm. Inhibition of SecA ATPase activity by azide blocks SodA export to the periplasm. | Periplasmic | G | N/A | Cytoplasmic | SEC | yes | yes | N | 17 | 0.122 | CYT | 0 | -0.200913 | - | 0 | 0 | N | i | Periplasmic | 9 | 77 | ||||||||||
| MLTC_ECOLI | P0C066 | mltC yggZ | yggZ | b2963 JW5481 | OM lipoprotein (SEC through IM), inserted in OM by LOL), Membrane-bound lytic murein transglycosylase C MltC | Outer Membrane Lipoprotein | Proven pignal peptidase II substrate. PMID: 15174130 | PMID: 15174130 PMID: 20932056 | Outer Membrane Lipoprotein | I | N/A | Cell outer membrane | Outer membrane Lipoprotein (Experimental) | SEC | LOL | yes | yes | Y | 24 | 0.3 | SpII | 16 | 14.8852 | Pos+2=S | 0 | 0 | Y | n5-15c20/21o | Unknown | 2 | 17 | ||||||
| MEPA_ECOLI | P0C0T5 | mepA | b2328 JW2325 | periplasmic protein (SEC through IM), Penicillin-insensitive murein endopeptidase MepA | Periplasmic | Substrate of periplasmic disulfide oxidoreductases DsbC and DsbA. Identified DsbC substrate PMID: 19953543 Identified DsbA substrate PMID: 19953543 PMID: 2187143 Disulfide bridge (44 265;187 235;216 223) Disulfide bond-containing protein PMID: 25664544 | PMID: 14726535 PMID: 19953543 PMID: 2187143 PMID: 25664544 | Periplasmic | G | N/A | Periplasm | Periplasmic (Experimental) | SEC | DSBA,DSBC | yes | yes | Y | 20 | 0.847 | SpI | 19 | 22.606 | - | 0 | 0 | Y | n4-14c19/20o | Periplasmic | 10 | 16 | |||||||
| DEGP_ECOLI | P0C0V0 | degP htrA ptd | htrA ptd | b0161 JW0157 | periplasmic protein (SEC through IM), serine endoprotease DegP | Periplasmic | SecB substrate. Substrate of periplasmic disulfide oxidoreductases DsbA. HtrA was not detected by the immunoblotting technique in the periplasm or in the fraction of soluble proteins but was found in the inner membrane. DegP oligomerizes as a hexameric cage but in its presence DegP reorganizes into 12 and 24-mer cages with large chambers that house the substrate for degradation or refolding. Size exclusion chromatography and electron microscopy show that the size of the substrate molecule is the main factor conditioning the oligomeric state adopted by the enzyme. Identified DsbA substrate PMID: 19953543 PMID: 2180903 Disulfide bridges between 83 and 95 PMID: 12878036 Disulfide bridge (83 95) PMID: 12878036 Disulfide bond-containing protein PMID: 25664544 | PMID: 12878036 PMID: 14726535 PMID: 16352602 PMID: 19525348 PMID: 19953543 PMID: 21370981 PMID: 21526129 PMID: 2180903 PMID: 25664544 PMID: 9083020 | Periplasmic | G | N/A | Cell inner membrane | Periplasmic (Experimental) | SECB | SEC | DSBA | yes | yes | Y | 27 | 0.841 | SpI | 26 | 28.3128 | - | 0 | 0 | Y | n4-19c26/27o | Periplasmic | 10 | 31 | |||||
| EMTA_ECOLI | P0C960 | emtA mltE sltZ ycgP | mltE sltZ ycgP | b1193 JW5821 | OM lipoprotein (SEC through IM), inserted in OM by LOL), Endo-type membrane-bound lytic murein transglycosylase A EmtA | Outer Membrane Lipoprotein | Proven pignal peptidase II substrate. PMID: 15174130 | PMID: 15174130 | Outer Membrane Lipoprotein | I | N/A | Cell outer membrane | Outer membrane Lipoprotein (Experimental) | SEC | LOL | yes | yes | Y | 49 | 0.204 | SpII | 15 | 18.8581 | Pos+2=S | 0 | 0 | Y | n5-13c18/19o | CytoplasmicMembrane | 9 | 15 | ||||||
| ACFD_ECOLI | P0CK95 | yghJ | b4466 JW5925 ECK2968 | IM lipoprotein (SEC through IM), inserted in OM by LOL), Putative AcfD homolog YghJ | Inner Membrane Lipoprotein | E | N/A | Cell membrane | Inner membrane lipoprotein | SEC | LOL | no | yes | Y | 23 | 0.283 | SpII | 23 | 37.591 | Pos+2=D | 0 | 0 | Y | n10-21c26/27o | Unknown | 6 | |||||||||||
| RLPA_ECOLI | P10100 | rlpA | b0633 JW0628 | OM lipoprotein (SEC through IM), inserted in OM by LOL), Endolytic PG transglycosylase RlpA RlpA | Outer Membrane Lipoprotein | Proven pignal peptidase II substrate. Outer membrane protein determined by LC-MS (Gevaert et al 2002). Outer membrane protein determined by 2D gel electrophoresis (Lopez-Campistrous et al 2005). Contains a SPOR domain. PMID: 15174130 | PMID: 12488465 PMID: 15174130 PMID: 15911532 PMID: 19880599 PMID: 20932056 | Outer Membrane Lipoprotein | I | N/A | Cell membrane | Outer membrane Lipoprotein (Experimental) | SEC | LOL | yes | yes | N | 20 | 0.14 | SpII | 17 | 19.3561 | Pos+2=T | 0 | 0 | Y | n5-15c20/21o | Unknown | 2 | ||||||||
| FADL_ECOLI | P10384 | fadL ttr | ttr | b2344 JW2341 | OM b-barrel protein (SEC through IM, inserted in OM by BAM), Long-chain fatty acid transport FadL | Outer Membrane b-barrel protein | Substrate of periplasmic peptidyl prolyl cis???trans isomerase SurA. Outer membrane protein determined by 2D gel electrophoresis (Lopez-Campistrous et al 2005) Potential Skp substrate PMID: 19953543 Identified SurA substrates PMID: 19953543 PMID: 1987139 | PMID: 10806384 PMID: 11266632 PMID: 12624733 PMID: 15178802 PMID: 15911532 PMID: 16079137 PMID: 19343722 PMID: 1987139 PMID: 19953543 PMID: 20932056 | Outer Membrane b-barrel protein | H | N/A | Cell outer membrane | Outer Membrane ??-barrel protein (Experimental) | SEC | SURA,SKP | BAM | yes | yes | Y | 26 | 0.796 | SpI | 25 | 16.4528 | - | 0 | 0 | Y | n10-20c25/26o | OuterMembrane | 10 | 1 | |||||
| IAP_ECOLI | P10423 | iap | b2753 JW2723 | periplasmic peripheral IM protein (SEC through IM), Alkaline phosphatase isozyme conversion Iap | Peripheral inner membrane protein facing the periplasm | PMID: 3316184 | Peripheral inner membrane protein facing the periplasm | F2 | N/A | Membrane associated (Experimental) | SEC | yes | yes | Y | 25 | 0.707 | SpI | 24 | 13.6553 | - | 0 | 0 | Y | n9-20c24/25o | Unknown | 4 | 25 | ||||||||||
| YFIL_ECOLI | P11289 | yfiL | b2602 JW5412 | OM lipoprotein (SEC through IM, inserted in OM by LOL), YfiL | Outer Membrane Lipoprotein | Proven pignal peptidase II substrate. PMID: 15174130 | PMID: 15174130 | Outer Membrane Lipoprotein | I | N/A | Outer membrane Lipoprotein (Experimental) | SEC | LOL | yes | yes | Y | 26 | 0.211 | SpII | 16 | 12.903 | Pos+2=Q | 0 | 0 | Y | n5-14c19/20o | Unknown | 2 | 59 | ||||||||
| FECA_ECOLI | P13036 | fecA | b4291 JW4251 | OM b-barrel protein (SEC through IM, inserted in OM by BAM), Fe(3+) dicitrate transport FecA | Outer Membrane b-barrel protein | Substrate of periplasmic peptidyl prolyl cis???trans isomerase SurA. Outer membrane protein determined by 2D gel electrophoresis (Lopez-Campistrous et al 2005, Fountoulakis et al 2003) Potential Skp substrate PMID: 19953543 Identified SurA substrates PMID: 19953543 PMID: 2836368 | PMID: 11266632 PMID: 11872840 PMID: 12624733 PMID: 15911532 PMID: 19343722 PMID: 19953543 PMID: 20932056 PMID: 2836368 | Outer Membrane b-barrel protein | H | N/A | Cell outer membrane | Outer Membrane ??-barrel protein (Experimental) | SEC | SURA,SKP | BAM | no | yes | Y | 34 | 0.831 | SpI | 33 | 18.814 | - | 0 | 0 | Y | n19-29c33/34o | OuterMembrane | 10 | 1 | ||||||
| TREA_ECOLI | P13482 | treA osmA | osmA | b1197 JW1186 | periplasmic protein (SEC through IM), trehalase TreA | Periplasmic | Potential Skp substrate PMID: 19953543 PMID: 2671658 Disulfide bond-containing protein PMID: 25664544 Disulfide bond-containing protein PMID: 25664544 | PMID: 19953543 PMID: 25664544 PMID: 2671658 | Periplasmic | G | N/A | Periplasm | Periplasmic (Experimental) | SEC | SKP | yes | yes | Y | 31 | 0.702 | SpI | 30 | 17.5054 | - | 0 | 0 | Y | n13-26c30/31o | Periplasmic | 10 | 70 | ||||||
| CHIA_ECOLI | P13656 | chiA yheB | yheB | b3338 JW3300 | periplasmic & extra-cellular protein (SEC through IM), TSS secreted over OM), Probable bifunctional chitinase/lysozyme ChiA | Periplasmic, Extra-cellular | Can be fully secreted when the cryptic T2S system is expressed. PMID: 10760150 Disulfide bridge (628 673) Disulfide bond-containing protein PMID: 25664544 | PMID: 10760150 PMID: 11118204 PMID: 25664544 | Periplasmic, Extra-cellular | G, X | N/A | Periplasm | Periplasmic (Experimental) | SEC | T2S | no | yes | Y | 25 | 0.672 | SpI | 24 | 20.7505 | - | 0 | 0 | Y | n11-20c24/25o | Periplasmic | 10 | |||||||
| FECB_ECOLI | P15028 | fecB | b4290 JW4250 | periplasmic & peripheral IM protein (SEC through IM), Fe(3+) dicitrate-binding periplasmic FecB | Peripheral inner membrane protein facing the periplasm, periplasmic | PMID: 2651410 | Peripheral inner membrane protein facing the periplasm, periplasmic | F2, G | N/A | Periplasm | Periplasmic (Experimental) | SEC | no | yes | Y | 22 | 0.843 | SpI | 21 | 8.42911 | - | 0 | 0 | Y | n7-16c21/22o | Periplasmic | 9 | 8 | |||||||||
| PHND_ECOLI | P16682 | phnD psiD | psiD | b4105 JW4066 | periplasmic protein (SEC through IM), Phosphonates-binding periplasmic protein PhnD | Periplasmic | PMID: 16751609 | Periplasmic | G | N/A | Periplasm | Periplasmic (Experimental) | SEC | yes | yes | Y | 27 | 0.567 | SpI | 26 | 16.4228 | - | 0 | 0 | Y | n6-21c26/27o | Periplasmic | 10 | |||||||||
| CYSP_ECOLI | P16700 | cysP | b2425 JW2418 | periplasmic protein (SEC through IM), Thiosulfate-binding protein CysP | Periplasmic | PMID: 9298646 | Periplasmic | G | N/A | Periplasm | Periplasmic (Experimental) | SEC | yes | yes | Y | 26 | 0.741 | SpI | 25 | 14.3932 | - | 0 | 0 | Y | n10-20c25/26o | Periplasmic | 10 | 61 | |||||||||
| FHUE_ECOLI | P16869 | fhuE | b1102 JW1088 | OM b-barrel protein (SEC through IM, inserted in OM by BAM), FhuE receptor FhuE | Outer Membrane b-barrel protein | Outer membrane protein determined by 2D gel electrophoresis (Lopez-Campistrous et al 2005, Fountoulakis et al 2003)','Outer membrane protein determined by 2D gel electrophoresis (Lopez-Campistrous et al 2005, Fountoulakis et al 2003, Molloy et al 2000) PMID: 3032906 | PMID: 10806384 PMID: 3032906 | Outer Membrane b-barrel protein | H | N/A | Cell outer membrane | Outer Membrane ??-barrel protein (Experimental) | SEC | BAM | no | yes | Y | 37 | 0.821 | SpI | 36 | 20.6615 | - | 0 | 0 | Y | n19-30c36/37o | OuterMembrane | 10 | 0 | |||||||
| CIRA_ECOLI | P17315 | cirA cir feuA | cir feuA | b2155 JW2142 | OM b-barrel protein (SEC through IM, inserted in OM by BAM), Colicin I receptor CirA | Outer Membrane b-barrel protein | Outer membrane protein determined by 2D gel electrophoresis (Lopez-Campistrous et al 2005, Fountoulakis et al 2003, Molloy et al 2000) Potential Skp substrate PMID: 19953543 Identified DsbA substrate PMID: 19953543 PMID: 10430870 Disulfide bond-containing protein PMID: 25664544 | PMID: 10430870 PMID: 10806384 PMID: 12624733 PMID: 15911532 PMID: 19953543 PMID: 20932056 PMID: 25664544 | Outer Membrane b-barrel protein | H | N/A | Cell outer membrane | Outer Membrane ??-barrel protein (Experimental) | SEC | DSBA,SKP | BAM | yes | yes | Y | 26 | 0.695 | SpI | 25 | 11.8271 | - | 0 | 0 | Y | n10-20c25/26o | OuterMembrane | 10 | 46 | |||||
| YFAA_ECOLI | P17994 | yfaA pufY | pufY | b2230 JW2224 | periplasmic protein (SEC through IM), YfaA | Periplasmic | G | Soluble IM Fraction | Periplasmic | SEC | no | yes | N | 31 | 0.233 | SpI | 30 | 5.42187 | - | 1 | 1 | N | i12-31o | CytoplasmicMembrane | 9 | 89 | |||||||||||
| PNCB_ECOLI | P18133 | pncB | b0931 JW0914 | periplasmic protein (SEC through IM), Nicotinate phosphoribosyltransferase PncB | Periplasmic | PncB is localized in the periplasm although it does not possess a signal peptide. | PMID: 346557 | Periplasmic | G | N/A | Cytoplasmic | SEC | no | yes | N | 61 | 0.11 | CYT | 0 | -0.200913 | - | 0 | 0 | N | i | Cytoplasmic | 9 | 32 | |||||||||
| YJJA_ECOLI | P18390 | yjjA | b4360 JW5795 | periplasmic protein (SEC through IM), YjjA | Periplasmic | Disulfide bond-containing protein PMID: 25664544 | PMID: 25664544 | Periplasmic | G | N/A | Periplasmic | SEC | yes | yes | Y | 26 | 0.86 | SpI | 25 | 23.6068 | - | 0 | 0 | Y | n8-19c25/26o | Unknown | 2 | 100 | |||||||||
| DMSA_ECOLI | P18775 | dmsA | b0894 JW5118 | periplasmic peripheral IM protein (TAT-SEC through IM), Dimethyl sulfoxide reductase DmsA | Peripheral inner membrane protein facing the periplasm | TAT signal peptide, dmsABC complex. Part of the anaerobic dimethylsulphoxide (DMSO) reductase DmsABC. The DmsC polypeptide is composed of eight segments of hydrophobic amino acids of appropriate length to cross the cytoplasmic membrane, suggesting that this subunit functions to anchor the DMSO enzyme to the membrane. PMID: 3062312 There are contradictory experiments although it seems that the senario of the DmsABC complex facing the cytoplasm is more favoured. PMID: 11389150 The hydrophobic region of the DmsA twin-arginine leader peptide determines specificity with chaperone DmsD. PMID: 24093457 Fluorescence analysis of these YFP chimeras revealed a wide range of interactions such as the one between the Tat substrate dimethyl sulfoxide reductase (DmsA) and its dedicated proofreading chaperone DmsD. PMID: 20169075 The localization of DmsAB was compared in a strain expressing a partial DmsC subunit (pDMSC59X) with full-length DmsC originating from the chromosomal copy of the dms operon. In those experiments some DmsAB was reported in the periplasm in the absence of full-length DmsC, leading us to suggest that DmsC might serve a stop-transfer role. PMID: 9546395 TTnphoA fusions, and electron paramagnetic resonance monitoring the effects of the probe dysprosium(III) on an engineered 3Fe4S cluster in DmsB indicated that DmsAB faces the cytoplasm PMID: 2170332 PMID: 3280546 PMID: 3062312 Interacts with DmsD, a redox enzyme maturation protein (REMP). PMID: 20153451 | PMID: 11389150 PMID: 17218314 PMID: 20153451 PMID: 20169075 PMID: 2170332 PMID: 24093457 PMID: 3062312 PMID: 3280546 PMID: 9546395 | Peripheral inner membrane protein facing the periplasm | F2 | Soluble IM Fraction | Cell membrane | Periplasmic (Experimental) | DmsD | TAT,SEC | yes | yes | N | 46 | 0.537 | SpI | 45 | 5.24058 | - | 0 | 0 | N | i | CytoplasmicMembrane | 10 | 22 | |||||||
| DMSB_ECOLI | P18776 | dmsB | b0895 JW0878 | periplasmic peripheral IM protein (TAT via piggyback through IM), Anaerobic dimethyl sulfoxide reductase chain B DmsB | Peripheral inner membrane protein facing the periplasm | TAT (piggyback); DmsB is co-exported bound to Tat substrate DmsA. Part of the anaerobic dimethylsulphoxide (DMSO) reductase DmsABC. The DmsC polypeptide is composed of eight segments of hydrophobic amino acids of appropriate length to cross the cytoplasmic membrane, suggesting that this subunit functions to anchor the DMSO enzyme to the membrane. PMID: 3062312 There are contradictory experiments although it seems that the senario of the DmsABC complex facing the cytoplasm is more favoured. PMID: 11389150 The localization of DmsAB was compared in a strain expressing a partial DmsC subunit (pDMSC59X) with full-length DmsC originating from the chromosomal copy of the dms operon. In those experiments some DmsAB was reported in the periplasm in the absence of full-length DmsC, leading us to suggest that DmsC might serve a stop-transfer role. PMID: 9546395 TTnphoA fusions, and electron paramagnetic resonance monitoring the effects of the probe dysprosium(III) on an engineered 3Fe4S cluster in DmsB indicated that DmsAB faces the cytoplasm PMID: 2170332 | PMID: 11389150 PMID: 2170332 PMID: 3062312 PMID: 9546395 | Peripheral inner membrane protein facing the periplasm | F2 | N/A | Cytoplasmic | TAT (piggyback) | yes | yes | N | 44 | 0.107 | CYT | 0 | -0.200913 | - | 0 | 0 | N | i | Unknown | 5 | 27 | |||||||||
| GGT_ECOLI | P18956 | ggt | b3447 JW3412 | periplasmic protein (SEC through IM), Glutathione hydrolase proenzyme Ggt | Periplasmic | Potential substrate of periplasmic chaperone YfgM. PMID: 25403562 Putative substrate for the periplasmic chaperone YfgM. PMID: 25403562 | PMID: 1360205 PMID: 25403562 PMID: 2570061 | Periplasmic | G | N/A | Periplasm | Periplasmic (Experimental) | SEC | YfgM | yes | yes | Y | 26 | 0.723 | SpI | 25 | 20.0548 | - | 0 | 0 | Y | n10-21c25/26o | Periplasmic | 10 | 36 | |||||||
| AGP_ECOLI | P19926 | agp | b1002 JW0987 | periplasmic protein (SEC through IM), Glucose-1-phosphatase Agp | Periplasmic | Disulfide bond-containing protein PMID: 25664544 | PMID: 2153660 PMID: 25664544 PMID: 9298646 | Periplasmic | G | N/A | Periplasm | Periplasmic (Experimental) | SEC | no | yes | Y | 23 | 0.88 | SpI | 22 | 21.9324 | - | 0 | 0 | Y | n5-16c22/23o | Periplasmic | 10 | 62 | ||||||||
| SPEA_ECOLI | P21170 | speA | b2938 JW2905 | periplasmic protein (SEC through IM), Biosynthetic arginine decarboxylase SpeA | Periplasmic | Trigger factor-sensitive aggregation. Experimentally detected by proteomics analysis of E.coli membrane fraction PMID: 19766000. No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Potential peripherally associated protein facing the cytoplasm or integral inner membrane protein. PMID: 2198270 Selective disruption of the cell envelope and an assessment of Spea activity or immunoprecipitable SpeA in various fractions demonstrated its location between the cytoplasmic membrane and peptidoglycan layer. PMID: 3894328 | PMID: 15919657 PMID: 19737520 PMID: 19766000 PMID: 2198270 PMID: 3894328 | Periplasmic | G | Soluble IM Fraction | Periplasm | Cytoplasmic | TF | SEC | yes | yes | N | 27 | 0.129 | CYT | 0 | -0.200913 | - | 0 | 0 | N | i | Periplasmic | 10 | 48 | |||||||
| RNI_ECOLI | P21338 | rna rnsA | rnsA | b0611 JW0603 | periplasmic protein (SEC through IM), Ribonuclease I Rna | Periplasmic | Substrate of periplasmic disulfide oxidoreductases DsbC and DsbA. Identified DsbA substrate PMID: 19953543 PMID: 2253883 Disulfide bridge (103 159) | PMID: 14726535 PMID: 19953543 PMID: 2253883 | Periplasmic | G | N/A | Periplasm Cytoplasm | Periplasmic (Experimental) | SEC | DSBA,DSBC | yes | yes | Y | 24 | 0.538 | SpI | 25 | 15.6073 | - | 0 | 0 | Y | n8-18c23/24o | Periplasmic | 10 | 31 | ||||||
| NMPC_ECOLI | P21420 | nmpC phmA | phmA | b0553 | OM b-barrel protein (SEC through IM, inserted in OM by BAM), Putative porin NmpC | Outer Membrane b-barrel protein | PMID: 337109 | Outer Membrane b-barrel protein | H | N/A | Cell outer membrane; Multi-pass membrane protein | Outer Membrane ss-barrel protein(experimental) | SEC | BAM | pseudogene (Uniprot) | yes | no | Y | 24 | 0.879 | SpI | 23 | 25.1335 | - | 0 | 0 | Y | n4-15c23/24o | OuterMembrane | 10 | 11 | ||||||
| YCBB_ECOLI | P22525 | ycbB | b0925 JW0908 | periplasmic peripheral OM protein (SEC through IM), Probable L,D-transpeptidase YcbB | Peripheral outer membrane protein facing the periplasm | YcbB forms direct meso- diaminopimelate (DAP-DAP, or 3-3) crosslinks within the peptidoglycan. | PMID: 23832002 | Peripheral outer membrane protein facing the periplasm | F3 | N/A | Membrane | Periplasmic | SEC | yes | yes | Y | 31 | 0.448 | SpI | 30 | 10.8337 | - | 0 | 0 | Y | n11-22c30/31o | Periplasmic | 9 | 63 | ||||||||
| ECOT_ECOLI | P23827 | eco eti | eti | b2209 JW2197 | periplasmic protein (SEC through IM), Eco | Periplasmic | Identified in periplasm by biochemical fractionation followed by two-dimensional gel electrophoresis and tandem mass spectrometry. PMID: 15911532 PMID: 9298646 Disulfide bridge (70 107) Disulfide bond-containing protein PMID: 25664544 | PMID: 15911532 PMID: 25664544 PMID: 9298646 | Periplasmic | G | N/A | Periplasm | Periplasmic (Experimental) | SEC | no | yes | Y | 21 | 0.889 | SpI | 20 | 16.5012 | - | 0 | 0 | Y | n4-15c20/21o | Periplasmic | 10 | 85 | |||||||
| OPPA_ECOLI | P23843 | oppA | b1243 JW1235 | periplasmic protein (SEC through IM), oligopeptide-binding protein OppA | Periplasmic | SecB substrate. Potential Skp substrate PMID: 19953543 Identified DsbA substrate PMID: 19953543 PMID: 9298646 Potential substrate for the SRP pathway. It was found to be down-regulated in the periplasm and up-regulated in the cytoplasm of SRP-depleted cells. PMID: 22030350 Disulfide bridge (297 443) Disulfide bond-containing protein PMID: 25664544 | PMID: 16352602 PMID: 19953543 PMID: 22030350 PMID: 25664544 PMID: 9298646 | Periplasmic | G | Soluble IM Fraction | Periplasm | Periplasmic (Experimental) | SECB | SEC | yes | yes | Y | 27 | 0.825 | SpI | 26 | 18.3169 | - | 1 | 0 | Y | n8-18c26/27o | Periplasmic | 10 | 73 | |||||||
| DPPA_ECOLI | P23847 | dppA | b3544 JW3513 | periplasmic protein (SEC through IM), dipeptide transport DppA | Periplasmic | Substrate of periplasmic disulfide oxidoreductases DsbA. Identified in periplasm by biochemical fractionation followed by two-dimensional gel electrophoresis and tandem mass spectrometry. PMID: 15911532 Potential Skp substrate PMID: 19953543 Identified DsbA substrate PMID: 19953543 PMID: 9298646 Disulfide bridge (34 262;450 463) Disulfide bond-containing protein PMID: 25664544 | PMID: 14726535 PMID: 15911532 PMID: 19953543 PMID: 25664544 PMID: 9298646 | Periplasmic | G | N/A | Periplasm | Periplasmic (Experimental) | SEC | DSBA,SKP | yes | yes | Y | 29 | 0.638 | SpI | 28 | 16.4393 | - | 0 | 0 | Y | n13-23c28/29o | Periplasmic | 10 | 85 | |||||||
| PSPE_ECOLI | P23857 | pspE | b1308 JW1301 | periplasmic protein (SEC through IM), Thiosulfate sulfurtransferase PspE | Periplasmic | Experimentally verified gene-product. Verified that the signal peptide is cleaved. PMID: 9868784 Disulfide bond-containing protein PMID: 25664544 | PMID: 1712397 PMID: 25664544 PMID: 9868784 | Periplasmic | G | N/A | Periplasm | Periplasmic (Experimental) | SEC | Prophage Transposase | yes | no | Y | 20 | 0.9 | SpI | 19 | 20.0627 | - | 0 | 0 | Y | n5-15c19/20o | Unknown | 2 | ||||||||
| PRC_ECOLI | P23865 | prc tsp | tsp | b1830 JW1819 | periplasmic peripheral IM protein (SEC through IM), Tail-specific protease Prc | Peripheral inner membrane protein facing the periplasm | Fractionation of the maxicells producing the product or prc gene suggested that the product was associated with the periplasmic side of the cytoplasmic membrane. The prc gene, which is involved in cleavage of the C-terminal peptide from the precursor form of penicillin-binding protein 3 (PBP 3) of Escherichia coli. PMID: 1856173 PMID: 1729701 | PMID: 1729701 PMID: 1856173 | Peripheral inner membrane protein facing the periplasm | F2 | N/A | Cell inner membrane; Peripheral membrane protein; Periplasmic side | Periplasmic (Experimental) | SEC | yes | yes | Y | 23 | 0.786 | SpI | 22 | 11.2952 | - | 0 | 0 | Y | n7-17c22/23o | CytoplasmicMembrane | 10 | ||||||||
| NLPC_ECOLI | P23898 | nlpC | b1708 JW1698 | OM lipoprotein (SEC through IM), inserted in OM by LOL), Probable endopeptidase NlpC | Outer Membrane Lipoprotein | Proven pignal peptidase II substrate. PMID: 15174130 | PMID: 15174130 | Outer Membrane Lipoprotein | I | N/A | Cell membrane | Outer membrane Lipoprotein (Experimental) | SEC | LOL | yes | yes | Y | 21 | 0.325 | SpII | 15 | 16.0698 | Pos+2=S | 0 | 0 | Y | n3-13c17/18o | Unknown | 2 | 34 | |||||||
| ACRE_ECOLI | P24180 | acrE envC | envC | b3265 JW3233 | IM lipoprotein (SEC through IM), inserted in OM by LOL), Multidrug export AcrE | Inner Membrane Lipoprotein | Proven pignal peptidase II substrate. | PMID: 15174130 PMID: 8472900 | Inner Membrane Lipoprotein | E | N/A | Cell inner membrane | Inner membrane lipoprotein | SEC | LOL | yes | yes | Y | 23 | 0.241 | SpII | 23 | 10.9032 | Pos+2=N | 0 | 0 | Y | n7-18c23/24o | CytoplasmicMembrane | 9 | 70 | ||||||
| FDNG_ECOLI | P24183 | fdnG | b1474 JW1470 | periplasmic peripheral IM protein (TAT through IM), Formate dehydrogenase, nitrate-inducible, major subunit FdnG | Peripheral inner membrane protein facing the periplasm | TAT secretion system; Formate dehydrogenase allows E.coli to use formate as major electron donor during anaerobic respiration, when nitrate is used as electron acceptor. The alpha subunit forms the active site. FdnG is the catalytic subunit of formate dehydrogenase-N (FdnGHI). FdnG was found in the periplasmic fraction of a mutant lacking the membrane anchor subunit FdnI, confirming that FdnG is located at the periplasmic face of the cytoplasmic membrane. PMID: 11929547 PMID: 11884747 PMID: 12923080 PMID: 11884747 PMID: 16858726 | PMID: 11884747 PMID: 11929547 PMID: 12923080 PMID: 15342602 PMID: 16858726 PMID: 17218314 | Peripheral inner membrane protein facing the periplasm | F2 | N/A | Periplasm | Membrane associated (Experimental) | TAT | no | yes | N | 34 | 0.342 | SpI | 33 | 6.74593 | - | 0 | 0 | Y | n11-24c33/34o | Periplasmic | 10 | |||||||||
| FLID_ECOLI | P24216 | fliD flaV flbC | flaV flbC | b1924 JW1909 | Flagellar extra-cellular peripheral OM protein (Flagellum secreted), hook-associated 2 FliD | Peripheral outer membrane protein facing the extra-cellular space | Filament capping protein. PMID: 7551038 PMID: 1527488 PMID: 6305924 | PMID: 1527488 PMID: 6305924 PMID: 7551038 | Peripheral outer membrane protein facing the extra-cellular space | F4 | N/A | Secreted Bacterial flagellum | Cell surface appendage (Experimental) | flagellum | Flagellum | Fla | no | yes | N | 25 | 0.12 | CYT | 0 | -0.200913 | - | 0 | 0 | N | i | Extracellular | 10 | 93 | |||||
| DACB_ECOLI | P24228 | dacB | b3182 JW3149 | periplasmic peripheral IM protein (SEC through IM), D-alanyl-D-alanine carboxypeptidase DacB | Peripheral inner membrane protein facing the periplasm | Exported from cytosol and signal peptide is cleaved. Anchored via C terminal the rest of the protein protudes into periplasm. Involved in peptidoglycan biosynthesis and is Penicillin-binding. PMID: 16411754 PMID: 15576782 DacB(PBP-4) anchors to the periplasmic face of the inner membrane via C-terminal amphiphilic alpha-helix. PMID: 9371419 | PMID: 12444970 PMID: 15576782 PMID: 15911532 PMID: 16411754 PMID: 9371419 | Peripheral inner membrane protein facing the periplasm | F2 | N/A | Periplasm | Membrane anchored (Experimental) | SEC | yes | yes | Y | 21 | 0.58 | SpI | 20 | 13.4163 | - | 0 | 0 | Y | n6-16c20/21o | Periplasmic | 9 | 34 | ||||||||
| ASLA_ECOLI | P25549 | aslA atsA | atsA | b3801 JW3773 | periplasmic protein (SEC through IM), Arylsulfatase AslA | Periplasmic | G | N/A | Periplasm | Periplasmic | SEC | no | yes | Y | 25 | 0.743 | SpI | 24 | 17.5099 | - | 0 | 0 | Y | n10-20c24/25o | Unknown | 6 | 25 | ||||||||||
| AMY1_ECOLI | P25718 | malS | b3571 JW3543 | periplasmic protein (SEC through IM), alpha-amylase MalS | Periplasmic | Disulfide bridge (57 75;121 537) PMID: 9268356 Disulfide bond-containing protein PMID: 25664544 | PMID: 1544897 PMID: 25664544 PMID: 9268356 | Periplasmic | G | N/A | Periplasm | Periplasmic (Experimental) | SEC | no | yes | Y | 18 | 0.764 | SpI | 17 | 14.5348 | - | 0 | 0 | Y | n3-11c17/18o | Periplasmic | 10 | 25 | ||||||||
| END1_ECOLI | P25736 | endA | b2945 JW2912 | periplasmic protein (SEC through IM), Endonuclease-1 EndA | Periplasmic | Identified DsbA substrate PMID: 19953543 PMID: 7867949 Disulfide bond-containing protein PMID: 25664544 | PMID: 19953543 PMID: 25664544 PMID: 4955462 PMID: 7867949 | Periplasmic | G | N/A | Periplasm | Periplasmic (Experimental) | SEC | DSBA | no | yes | Y | 23 | 0.883 | SpI | 22 | 22.5344 | - | 0 | 0 | Y | n5-16c22/23o | Unknown | 5 | 99 | |||||||
| LOIP_ECOLI | P25894 | loiP yggG | yggG | b2936 JW2903 | OM lipoprotein (SEC through IM), inserted in OM by LOL), Metalloprotease LoiP | Outer Membrane Lipoprotein | Disulfide bridge (53 108) PMID: 22491786 | PMID: 17909889 PMID: 20932056 PMID: 22491786 | Outer Membrane Lipoprotein | I | N/A | Cell outer membrane | Outer membrane Lipoprotein | SEC | LOL | yes | yes | N | 35 | 0.146 | SpII | 18 | 25.8287 | Pos+2=Q | 0 | 0 | Y | n5-16c34/35o | Unknown | 2 | 60 | ||||||
| YDBD_ECOLI | P25907 | ydbD | b1407 JW5221 | periplasmic protein (SEC through IM), YdbD | Periplasmic | G | N/A | Periplasmic | SEC | no | yes | Y | 25 | 0.744 | SpI | 24 | 7.29978 | - | 0 | 0 | Y | n12-20c24/25o | CytoplasmicMembrane | 9 | 28 | ||||||||||||
| BGLH_ECOLI | P26218 | bglH yieC | yieC | b3720 JW3698 | OM b-barrel protein (SEC through IM, inserted in OM by BAM), Cryptic porin BglH | Outer Membrane b-barrel protein | Reconstitution experiments with lipid bilayer membranes defined BglH as a channel-forming component, i.e. it is an outer membrane porin. Can be easily removed from the outer membrane by treatment with detergent. PMID: 10027967 PMID: 10027967 | PMID: 10027967 | Outer Membrane b-barrel protein | H | N/A | Cell outer membrane | Outer Membrane ??-barrel protein (Experimental) | SEC | BAM | no | yes | Y | 26 | 0.64 | SpI | 25 | 13.3498 | - | 0 | 0 | Y | n6-17c25/26o | OuterMembrane | 10 | 8 | ||||||
| AMIB_ECOLI | P26365 | amiB yjeD | yjeD | b4169 JW4127 | periplasmic protein (SEC through IM), N-acetylmuramoyl-L-alanine amidase AmiB | Periplasmic | PMID: 12787347 | Periplasmic | G | N/A | Periplasm | Periplasmic (Experimental) | SEC | yes | yes | Y | 23 | 0.861 | SpI | 22 | 13.4392 | - | 0 | 0 | Y | n9-17c22/23o | Unknown | 2 | 30 | ||||||||
| FTSP_ECOLI | P26648 | ftsP sufI | sufI | b3017 JW2985 | periplasmic protein (TAT-SEC through IM), Cell division FtsP | Periplasmic | TAT secretion system. PMID: 9298646 | PMID: 10593889 PMID: 17218314 PMID: 19135451 PMID: 9298646 | Periplasmic | G | N/A | Periplasm | Periplasmic (Experimental) | TAT,SEC | no | yes | Y | 28 | 0.494 | SpI | 27 | 10.6124 | - | 0 | 0 | Y | n8-19c27/28o | Periplasmic | 10 | 8 | |||||||
| YIGE_ECOLI | P27840 | yigE | b4482 JW5591 | periplasmic protein (SEC through IM), YigE | Periplasmic | Potential substrate of periplasmic peptidyl prolyl cis???trans isomerase SurA; sequence contains known binding motif of SurA [RYEF]X[RYEF][P]. | PMID: 18498364 | Periplasmic | G | N/A | Periplasmic | SEC | SURA | pseudogene (Ochman & Davalos, 2006) | no | no | Y | 33 | 0.615 | SpI | 32 | 10.2626 | - | 0 | 0 | Y | n19-29c33/34o | Unknown | 2 | ||||||||
| MLIC_ECOLI | P28224 | mliC ydhA | ydhA | b1639 JW1631 | OM lipoprotein (SEC through IM), inserted in OM by LOL), Membrane-bound lysozyme inhibitor of C-type lysozyme MliC | Outer Membrane Lipoprotein | Disulfide bridge (37 102) PMID: 16937244 | PMID: 16937244 PMID: 18369469 | Outer Membrane Lipoprotein | I | N/A | Cell outer membrane | Outer membrane Lipoprotein | SEC | LOL | yes | yes | Y | 23 | 0.288 | SpII | 17 | 7.61087 | Pos+2=S | 0 | 0 | Y | n6-15c20/21o | Unknown | 2 | 53 | ||||||
| CSGA_ECOLI | P28307 | csgA | b1042 JW1025 | Curli related extra-cellular peripheral OM protein (SEC through IM), secreted over OM by curli), Major curlin subunit CsgA | Peripheral outer membrane protein facing the extra-cellular space | Curli component. CsgA, is secreted from the cell in an unpolymerized form PMID: 11823641 The secretion and stability of CsgA and CsgB are dependent on the outer membrane lipoprotein CsgG. PMID: 21645131 Curli are a functional amyloid whose in vivo polymerization requires a dedicated nucleator protein, CsgB, and an assembly protein, CsgF. Without CsgF, curli subunits are released from the cell into the media and are inefficiently polymerized, resulting in fewer and mislocalized curli fibers. PMID: 19131513 PMID: 9383186 PMID: 9312041 During a process called interbacterial complementation, a csgB mutant cell secretes soluble CsgA that can be assembled on the surface of a cell expressing only csgB PMID: 8817489 High levels of both IbsC and ShoB lead to changes in membrane potential suggesting that both act at the membrane, consistent with the hydrophobic nature of the proteins. | PMID: 11823641 PMID: 16704339 PMID: 18710431 PMID: 19131513 PMID: 21645131 PMID: 8817489 PMID: 9312041 PMID: 9383186 | Peripheral outer membrane protein facing the extra-cellular space | F4 | N/A | Fimbrium | Cell surface appendage (Experimental) | Curli | SEC | Curli | yes | yes | Y | 21 | 0.854 | SpI | 20 | 20.7833 | - | 0 | 0 | Y | n3-14c20/21o | Extracellular | 9 | 66 | ||||||
| METQ_ECOLI | P28635 | metQ yaeC | yaeC | b0197 JW0193 | IM lipoprotein (SEC through IM), inserted in OM by LOL), D-methionine-binding MetQ | Inner Membrane Lipoprotein | Proven pignal peptidase II substrate. PMID: 12218041 | PMID: 12218041 PMID: 15174130 | Inner Membrane Lipoprotein | E | Soluble IM Fraction | Cell membrane | Inner membrane lipoprotein (Experimental) | SEC | LOL | yes | yes | N | 25 | 0.18 | SpII | 22 | 19.6725 | Pos+2=G | 0 | 0 | Y | n7-18c23/24o | CytoplasmicMembrane | 9 | 85 | ||||||
| YAAI_ECOLI | P28696 | yaaI | b0013 JW0012 | periplasmic protein (SEC through IM), UPF0412 YaaI | Periplasmic | G | N/A | Periplasmic | SEC | yes | yes | Y | 24 | 0.727 | SpI | 23 | 9.88216 | - | 0 | 0 | Y | n4-15c23/24o | Unknown | 2 | 59 | ||||||||||||
| GLTF_ECOLI | P28721 | gltF | b3214 JW3181 | Fimbrial periplasmic protein (SEC through IM), GltF | Periplasmic | Putative chaperone???usher fimbrial operon (class ??). Fimbrial Subunit. PMID: 10481090 | PMID: 10481090 PMID: 20345943 PMID: 21890895 | Periplasmic | G | N/A | Cell membrane | Periplasmic (Experimental) | fimbriae | SEC | no | yes | Y | 26 | 0.754 | SpI | 25 | 26.3964 | - | 0 | 0 | Y | n7-18c25/26o | Unknown | 2 | 95 | |||||||
| YHCA_ECOLI | P28722 | yhcA | b3215 JW3182 | Fimbrial periplasmic protein (SEC through IM), Chaperone YhcA | Periplasmic | Potential fimbrial yhc operon (class ??); Usher. | PMID: 20345943 | Periplasmic | G | N/A | Periplasm | Periplasmic | fimbriae | SEC | no | yes | N | 20 | 0.315 | CYT | 0 | -0.200913 | - | 0 | 0 | Y | n5-13c21/22o | Cytoplasmic | 8 | 26 | |||||||
| YBFC_ECOLI | P28915 | ybfC | b0704 JW0693 | periplasmic protein (SEC through IM), YbfC | Prediction of three weak transmembrane helixes (one with 0.6 probability) in Phobius. | Periplasmic | G | N/A | Periplasmic | SEC | no | yes | Y | 20 | 0.787 | SpI | 19 | 3.48234 | - | 0 | 0 | Y | n4-15c19/20o | Unknown | 2 | 48 | |||||||||||
| FLGL_ECOLI | P29744 | flgL flaT flaU | flaT flaU | b1083 JW1070 | Flagellar extra-cellular peripheral OM protein (Flagellum secreted), hook-associated 3 FlgL | Peripheral outer membrane protein facing the extra-cellular space | Second hook-filament junction protein. PMID: 12730325 | PMID: 12631703 PMID: 12730325 | Peripheral outer membrane protein facing the extra-cellular space | F4 | N/A | Secreted Bacterial flagellum | Cell surface appendage (Experimental) | flagellum | Flagellum | Fla | yes | yes | N | 58 | 0.149 | CYT | 0 | -0.200913 | - | 0 | 0 | N | i | Extracellular | 10 | 35 | |||||
| FIMD_ECOLI | P30130 | fimD | b4317 JW5780 | Fimbrial OM b-barrel protein (SEC through IM, chaperone usher export system in OM), FimD | Outer Membrane b-barrel protein | Fim operon (class ??1); Usher. ??uter membrane protein related to resistance to ampicillin and tetracycline. Outer membrane protein determined by LC-MS (Gevaert et al 2002) Outer membrane protein determined by 2D gel electrophoresis (Lopez-Campistrous et al 2005, Fountoulakis et al 2003) Identified in the outer membrane by biochemical fractionation followed by two-dimensional gel electrophoresis and tandem mass spectrometry. PMID: 15911532 PMID: 12842468 Disulfide bridge (855 877) | PMID: 12488465 PMID: 12624733 PMID: 12842468 PMID: 15911532 PMID: 16372265 PMID: 20345943 PMID: 24140205 | Outer Membrane b-barrel protein | H | N/A | Cell outer membrane | Outer Membrane ??-barrel protein (Experimental) | fimbriae | SEC | CU | yes | yes | Y | 38 | 0.415 | SpI | 37 | 1.20154 | - | 1 | 0 | Y | n23-38c45/46o | OuterMembrane | 10 | |||||||
| ARTI_ECOLI | P30859 | artI | b0863 JW0847 | periplasmic protein (SEC through IM), Putative ABC transporter arginine-binding 2 ArtI | Periplasmic | Identified in periplasm by biochemical fractionation followed by two-dimensional gel electrophoresis and tandem mass spectrometry. PMID: 15911532 Identified in periplasm by biochemical fractionation followed by two-dimensional gel electrophoresis and tandem mass spectrometry. PMID: 15911532 PMID: 8801422 PMID: 9298646 Potential substrate for the SRP pathway. It was found to be down-regulated in the periplasm and up-regulated in the cytoplasm of SRP-depleted cells. PMID: 22030350 Disulfide bond-containing protein PMID: 25664544 | PMID: 15911532 PMID: 22030350 PMID: 25664544 PMID: 8801422 PMID: 9298646 | Periplasmic | G | N/A | Periplasm | Periplasmic (Experimental) | SEC | yes | yes | Y | 20 | 0.883 | SpI | 19 | 18.5986 | - | 0 | 0 | Y | n4-15c19/20o | Periplasmic | 10 | 76 | ||||||||
| ARTJ_ECOLI | P30860 | artJ | b0860 JW0844 | periplasmic protein (SEC through IM), ABC transporter arginine-binding 1 ArtJ | Periplasmic | Identified DsbA substrate PMID: 19953543 PMID: 8801423 Potential substrate for the SRP pathway. It was found to be down-regulated in the periplasmic compartment of SRP-depleted cells. PMID: 22030350 | PMID: 19953543 PMID: 22030350 PMID: 8801422 PMID: 8801423 | Periplasmic | G | N/A | Periplasm | Periplasmic (Experimental) | SEC | DSBA | yes | yes | Y | 20 | 0.849 | SpI | 19 | 18.9337 | - | 0 | 0 | Y | n4-15c19/20o | Periplasmic | 10 | 73 | |||||||
| YADC_ECOLI | P31058 | yadC | b0135 JW0131 | Fimbrial extra-cellular peripheral OM protein (SEC through IM, chaperone usher export system over OM), YadC | Peripheral outer membrane protein facing the extra-cellular space | Potential fimbrial yad operon (class ??4); Potential tip-adhesin. | PMID: 19820722 PMID: 20345943 | Peripheral outer membrane protein facing the extra-cellular space | F4 | N/A | Fimbrium | Periplasmic | fimbriae | SEC | CU | no | yes | Y | 23 | 0.677 | SpI | 22 | 12.8917 | - | 1 | 0 | Y | n8-17c22/23o | Extracellular | 9 | 14 | ||||||
| YEDD_ECOLI | P31063 | yedD | b1928 JW1913 | OM lipoprotein (SEC through IM, inserted in OM by LOL), YedD | Outer Membrane Lipoprotein | Proven pignal peptidase II substrate. Outer membrane protein determined by LC-MS (Gevaert et al 2002). Outer membrane protein determined by 2D gel electrophoresis (Lopez-Campistrous et al 2005) Identified DsbA substrate PMID: 19953543 PMID: 15174130 Disulfide bond-containing protein PMID: 25664544 | PMID: 12488465 PMID: 15174130 PMID: 15911532 PMID: 19953543 PMID: 25664544 | Outer Membrane Lipoprotein | I | Soluble IM Fraction | Cell membrane | Outer membrane Lipoprotein (Experimental) | SEC | DSBA | LOL | no | yes | Y | 20 | 0.338 | SpII | 15 | 12.6143 | Pos+2=A | 0 | 0 | Y | n4-12c16/17o | Unknown | 2 | 130 | ||||||
| MARB_ECOLI | P31121 | marB | b1532 JW1525 | periplasmic protein (SEC through IM), Multiple antibiotic resistance MarB | Periplasmic | G | N/A | Periplasmic | SEC | no | yes | Y | 22 | 0.602 | SpI | 21 | 18.3001 | - | 0 | 0 | Y | n4-15c21/22o | Unknown | 2 | 60 | ||||||||||||
| YDEI_ECOLI | P31130 | ydeI | b1536 JW1529 | periplasmic protein (SEC through IM), YdeI | Periplasmic | G | N/A | Periplasmic | SEC | no | yes | Y | 20 | 0.882 | SpI | 19 | 13.7979 | - | 0 | 0 | Y | n3-14c19/20o | Unknown | 2 | 100 | ||||||||||||
| POTF_ECOLI | P31133 | potF | b0854 JW0838 | periplasmic protein (SEC through IM), Putrescine-binding periplasmic protein PotF | Periplasmic | Disulfide bridge (175 239) PMID: 9651355 Disulfide bond-containing protein PMID: 25664544 | PMID: 25664544 PMID: 9298646 PMID: 9651355 | Periplasmic | G | N/A | Periplasm | Periplasmic (Experimental) | SEC | yes | yes | Y | 27 | 0.731 | SpI | 26 | 15.245 | - | 0 | 0 | Y | n9-20c26/27o | Periplasmic | 10 | 61 | ||||||||
| YIEL_ECOLI | P31471 | yieL | b3719 JW5612 | periplasmic protein (SEC through IM), YieL | Periplasmic | G | N/A | Periplasmic | SEC | no | yes | Y | 24 | 0.659 | SpI | 23 | 16.8534 | - | 0 | 0 | Y | n5-16c23/24o | Unknown | 2 | |||||||||||||
| EFEB_ECOLI | P31545 | efeB ycdB | ycdB | b1019 JW1004 | periplasmic protein (TAT-SEC through IM), Deferrochelatase/peroxidase EfeB | Periplasmic | TAT secretion system. | PMID: 16551627 PMID: 17218314 | Periplasmic | G | N/A | Periplasm | Periplasmic | TAT,SEC | yes | yes | Y | 36 | 0.748 | SpI | 35 | 5.64598 | - | 0 | 0 | Y | n20-30c35/36o | Periplasmic | 10 | 33 | |||||||
| THIB_ECOLI | P31550 | thiB tbpA yabL | tbpA yabL | b0068 JW0067 | periplasmic protein (SEC through IM), Thiamine-binding periplasmic protein ThiB | Periplasmic | Potential substrate of periplasmic peptidyl prolyl cis???trans isomerase SurA; sequence contains known binding motif of SurA [RYEF]X[RYEF][P]. Disulfide bond-containing protein PMID: 25664544 | PMID: 18498364 PMID: 25664544 | Periplasmic | G | N/A | Periplasm | Periplasmic | SEC | SURA | no | yes | Y | 19 | 0.685 | SpI | 18 | 10.962 | - | 0 | 0 | Y | n5-13c18/19o | Periplasmic | 10 | 22 | ||||||
| LPTD_ECOLI | P31554 | lptD imp ostA yabG | imp ostA yabG | b0054 JW0053 | OM b-barrel protein (SEC through IM, inserted in OM by BAM), LPS-assembly LptD | Outer Membrane b-barrel protein | The Protein-disulfide Isomerase DsbC Cooperates with SurA and DsbA in the Assembly of LptD. Outer membrane protein determined by 2D gel electrophoresis (Lopez-Campistrous et al 2005) Potential Skp substrate PMID: 19953543 Identified SurA substrates PMID: 19953543 Identified DsbA substrate PMID: 19953543 PMID: 9298646 Disulfide bridge (31 724;173 725) | PMID: 12724388 PMID: 15911532 PMID: 16079137 PMID: 19343722 PMID: 19953543 PMID: 20615876 PMID: 20932056 PMID: 22901060 PMID: 9298646 | Outer Membrane b-barrel protein | H | Soluble IM Fraction | Cell outer membrane | Outer Membrane ??-barrel protein (Experimental) | SEC | DSBA,DSBC,SURA,SKP | BAM | yes | yes | Y | 25 | 0.626 | SpI | 24 | 18.6702 | - | 0 | 0 | Y | n7-17c24/25o | OuterMembrane | 10 | ||||||
| NFRA_ECOLI | P31600 | nfrA | b0568 JW0557 | OM b-barrel protein (SEC through IM, inserted in OM by BAM), Bacteriophage adsorption A NfrA | Outer Membrane b-barrel protein | Outer membrane protein determined by 2D gel electrophoresis (Lopez-Campistrous et al 2005, Fountoulakis et al 2003) | PMID: 12624733 | Outer Membrane b-barrel protein | H | N/A | Cell outer membrane | Outer Membrane ??-barrel protein | SEC | BAM | no | yes | Y | 28 | 0.836 | SpI | 27 | 8.97469 | - | 0 | 0 | Y | n10-21c27/28o | OuterMembrane | 10 | 4 | |||||||
| YADE_ECOLI | P31666 | yadE | b0130 JW0126 | periplasmic peripheral IM protein (SEC through IM), YadE | Homolog to C-terminus of DnaA which is a peripherally accossiated proteins with a C-terminal anchor. | Peripheral inner membrane protein facing the periplasm | F2 | N/A | Periplasmic | SEC | yes | yes | Y | 20 | 0.838 | SpI | 19 | 12.5161 | - | 1 | 0 | Y | n5-15c19/20o | Unknown | 2 | 12 | |||||||||||
| FIMC_ECOLI | P31697 | fimC | b4316 JW4279 | Fimbrial periplasmic protein (SEC through IM), Chaperone FimC | Periplasmic | Fim operon (class ??1); Chaperone. Potential substrate of periplasmic peptidyl prolyl cis???trans isomerase SurA; sequence contains known binding motif of SurA [RYEF]X[RYEF][P]. PMID: 1363735 | PMID: 1363735 PMID: 18498364 PMID: 20345943 PMID: 24140205 | Periplasmic | G | N/A | Periplasm | Periplasmic (Experimental) | fimbriae | SEC | SURA | yes | yes | N | 37 | 0.473 | SpI | 36 | 11.9808 | - | 1 | 0 | Y | n14-23c41/42o | Periplasmic | 10 | |||||||
| YDDB_ECOLI | P31827 | yddB | b1495 JW1490 | OM b-barrel protein (SEC through IM), YddB | Outer Membrane b-barrel protein | H | N/A | Periplasmic | SEC | no | yes | N | 20 | 0.536 | SpI | 19 | 4.63803 | - | 0 | 0 | Y | n4-14c19/20o | OuterMembrane | 9 | 3 | ||||||||||||
| PQQL_ECOLI | P31828 | pqqL yddC | yddC | b1494 JW1489 | periplasmic protein (SEC through IM), Probable zinc protease PqqL | Periplasmic | Potential substrate of periplasmic peptidyl prolyl cis???trans isomerase SurA; sequence contains known binding motif of SurA [RYEF]X[RYEF][P]. | PMID: 18498364 | Periplasmic | G | Soluble IM Fraction | Cytoplasmic | SEC | SURA | no | yes | N | 27 | 0.318 | SpI | 26 | 5.54177 | - | 1 | 0 | Y | n8-20c26/27o | CytoplasmicMembrane | 9 | 37 | |||||||
| YDEK_ECOLI | P32051 | ydeK orfT | orfT | b1510 JW1503 | OM lipoprotein (SEC through IM, inserted in OM by LOL), YdeK | Outer Membrane Lipoprotein | I | N/A | Cell membrane | Outer membrane Lipoprotein | SEC | LOL | pseudogene (Uniprot) | no | no | N | 62 | 0.164 | SpII | 18 | 0.949979 | Pos+2=S | 0 | 0 | N | i | OuterMembrane | 9 | |||||||||
| YIBG_ECOLI | P32106 | yibG | b3596 JW3570 | periplasmic protein (SEC through IM), YibG | Periplasmic | G | N/A | Periplasmic | SEC | no | yes | Y | 20 | 0.567 | SpI | 19 | 0.904394 | - | 0 | 0 | Y | n3-14c19/20o | Unknown | 2 | 18 | ||||||||||||
| YIIG_ECOLI | P32151 | yiiG | b3896 JW3867 | IM lipoprotein (SEC through IM, inserted in OM by LOL), YiiG | Inner Membrane Lipoprotein | Proven pignal peptidase II substrate. | PMID: 15174130 | Inner Membrane Lipoprotein | E | N/A | Inner membrane lipoprotein | SEC | LOL | no | yes | N | 22 | 0.152 | SpII | 21 | 26.248 | Pos+2=D | 0 | 0 | Y | n5-17c22/23o | Unknown | 4 | 106 | ||||||||
| YIIQ_ECOLI | P32160 | yiiQ | b3920 JW3891 | periplasmic protein (SEC through IM), YiiQ | Periplasmic | G | N/A | Periplasmic | SEC | yes | yes | Y | 24 | 0.736 | SpI | 23 | 14.7775 | - | 0 | 0 | Y | n4-15c23/24o | Unknown | 2 | |||||||||||||
| YIIX_ECOLI | P32167 | yiiX | b3937 JW3908 | periplasmic protein (SEC through IM), YiiX | Periplasmic | G | N/A | Periplasmic | SEC | no | yes | Y | 19 | 0.818 | SpI | 18 | 14.7441 | - | 0 | 0 | Y | n5-13c18/19o | Unknown | 2 | 27 | ||||||||||||
| FDOG_ECOLI | P32176 | fdoG | b3894 JW3865 | periplasmic protein (TAT through IM), Formate dehydrogenase-O major subunit FdoG | Periplasmic | TAT secretion system. PMID: 12923080 | PMID: 12923080 PMID: 17218314 | Periplasmic | G | Soluble IM Fraction | Periplasm | Periplasmic (Experimental) | TAT | no | yes | N | 34 | 0.487 | SpI | 33 | 14.6779 | - | 0 | 0 | Y | n11-24c33/34o | Periplasmic | 10 | |||||||||
| YIJF_ECOLI | P32668 | yijF | b3944 JW3916 | periplasmic protein (SEC through IM), YijF | Periplasmic | G | N/A | Periplasmic | SEC | yes | yes | Y | 19 | 0.257 | SpI | 20 | 11.5911 | - | 0 | 0 | Y | n3-14c18/19o | Unknown | 2 | 14 | ||||||||||||
| YJAH_ECOLI | P32681 | yjaH | b4001 JW3965 | OM lipoprotein (SEC through IM, inserted in OM by LOL), YjaH | Outer Membrane Lipoprotein | I | N/A | Outer membrane Lipoprotein | SEC | LOL | yes | yes | N | 36 | 0.151 | SpII | 30 | 6.9142 | Pos+2=S | 0 | 0 | Y | n20-28c46/47o | Unknown | 2 | 25 | |||||||||||
| YJBF_ECOLI | P32687 | yjbF | b4027 JW5711 | OM lipoprotein (SEC through IM, inserted in OM by LOL), YjbF | Outer Membrane Lipoprotein | Proven pignal peptidase II substrate. PMID: 15174130 | PMID: 15174130 | Outer Membrane Lipoprotein | I | N/A | Cell membrane | Outer membrane Lipoprotein (Experimental) | SEC | LOL | yes | yes | Y | 21 | 0.427 | SpII | 15 | 13.4041 | Pos+2=S | 0 | 0 | Y | n5-13c18/19o | Unknown | 2 | ||||||||
| YJBG_ECOLI | P32688 | yjbG | b4028 JW3988 | periplasmic protein (SEC through IM), YjbG | Periplasmic | G | N/A | Periplasmic | SEC | yes | yes | Y | 21 | 0.827 | SpI | 20 | 17.0485 | - | 0 | 0 | Y | n5-15c20/21o | CytoplasmicMembrane | 9 | |||||||||||||
| YJBH_ECOLI | P32689 | yjbH | b4029 JW3989 | IM lipoprotein (SEC through IM, inserted in OM by LOL), YjbH | Inner Membrane Lipoprotein | E | N/A | Cell membrane | Membrane Lipoprotein | SEC | LOL | yes | yes | Y | 21 | 0.41 | SpII | 17 | 12.8674 | Pos+2=Y | 0 | 0 | Y | n6-14c20/21o | OuterMembrane | 9 | |||||||||||
| NRFG_ECOLI | P32712 | nrfG yjcN | yjcN | b4076 JW4037 | OM lipoprotein (SEC through IM), inserted in OM by LOL), Formate-dependent nitrite reductase complex subunit NrfG | Outer Membrane Lipoprotein | I | N/A | Outer membrane Lipoprotein | SEC | LOL | yes | yes | N | 35 | 0.209 | SpII | 21 | 6.47674 | Pos+2=V | 1 | 0 | Y | n11-22c30/31o | Unknown | 2 | 64 | ||||||||||
| MDTP_ECOLI | P32714 | mdtP yjcP | yjcP | b4080 JW4041 | OM lipoprotein (SEC through IM), inserted in OM by LOL), Multidrug resistance MdtP | Outer Membrane Lipoprotein | I | N/A | Cell outer membrane | Outer membrane Lipoprotein | SEC | LOL | yes | yes | N | 23 | 0.147 | SpII | 23 | 4.25545 | Pos+2=A | 0 | 0 | Y | n9-21c29/30o | OuterMembrane | 9 | 12 | |||||||||
| YJCS_ECOLI | P32717 | yjcS | b4083 JW5721 | periplasmic protein (SEC through IM), Putative alkyl/aryl-sulfatase YjcS | Periplasmic | Periplasmic | G | N/A | Periplasmic | SEC | yes | yes | Y | 30 | 0.725 | SpI | 29 | 16.7747 | - | 0 | 0 | Y | n12-20c29/30o | Unknown | 4 | 27 | |||||||||||
| DACD_ECOLI | P33013 | dacD phsE yeeC | phsE yeeC | b2010 JW5329 | periplasmic peripheral IM protein (SEC through IM), D-alanyl-D-alanine carboxypeptidase DacD | Peripheral inner membrane protein facing the periplasm | Penicillin-binding protein 5 (PBP 5); membrane-bound cell wall dd-carboxypeptidase, localized in the outer leaflet of the cytosolic membrane. PMID: 8955390 | PMID: 20192190 PMID: 8955390 | Peripheral inner membrane protein facing the periplasm | F2 | N/A | Cell inner membrane | Membrane anchored (Experimental) | SEC | yes | yes | Y | 22 | 0.485 | SpI | 21 | 12.6922 | - | 0 | 0 | Y | n5-16c21/22o | CytoplasmicMembrane | 9 | 12 | |||||||
| YADV_ECOLI | P33128 | yadV ecpD | ecpD | b0140 JW0136 | Fimbrial periplasmic protein (SEC through IM), Probable chaperone YadV | Periplasmic | Putative chaperone???usher fimbrial operon (class ??4); Chaperone. Potential substrate of periplasmic peptidyl prolyl cis???trans isomerase SurA; sequence contains known binding motif of SurA [RYEF]X[RYEF][P]. | PMID: 18498364 PMID: 20345943 | Periplasmic | G | N/A | Periplasm | Periplasmic | fimbriae | SEC | SURA | no | yes | Y | 26 | 0.667 | SpI | 25 | 16.6944 | - | 0 | 0 | Y | n8-19c25/26o | Periplasmic | 10 | ||||||
| HTRE_ECOLI | P33129 | htrE | b0139 JW0135 | Fimbrial OM b-barrel protein (SEC through IM, inserted in OM by BAM), HtrE | Outer Membrane b-barrel protein | Putative chaperone???usher fimbrial operon (class ??4); Usher. Disulfide bridge (838 862) | PMID: 20345943 | Outer Membrane b-barrel protein | H | N/A | Cell outer membrane | Outer Membrane ??-barrel protein | fimbriae | SEC | BAM | no | yes | Y | 30 | 0.677 | SpI | 29 | 6.72527 | - | 0 | 0 | N | i | OuterMembrane | 10 | 8 | ||||||
| OPGG_ECOLI | P33136 | mdoG opgG | opgG | b1048 JW1035 | periplasmic protein (SEC through IM), Glucans biosynthesis G MdoG | Periplasmic | Copurifies with trigger factor in pull down assays. Potential Skp substrate PMID: 19953543 PMID: 9298646 | PMID: 19737520 PMID: 19953543 PMID: 9298646 | Periplasmic | G | N/A | Periplasm | Periplasmic (Experimental) | TF | SEC | SKP | yes | yes | Y | 23 | 0.708 | SpI | 22 | 16.361 | - | 0 | 0 | Y | n6-17c22/23o | Periplasmic | 10 | 23 | |||||
| YEBF_ECOLI | P33219 | yebF | b1847 JW1836 | extra-cellular protein (SEC through IM, secreted in OMV), YebF | Extra-cellular | Outer membrane proteins OmpF, OmpC, and OmpX are central to the YebF export mechanism. Identified DsbA substrate PMID: 19953543 PMID: 16369539 Disulfide bond-containing protein PMID: 25664544 | PMID: 16369539 PMID: 19953543 PMID: 22658749 PMID: 25664544 | Extra-cellular | X | N/A | Secreted | Secreted and released (Experimental) | SEC | OMV | no | yes | Y | 22 | 0.777 | SpI | 21 | 20.4773 | - | 0 | 0 | Y | n5-16c21/22o | Extracellular | 10 | 108 | |||||||
| TORA_ECOLI | P33225 | torA | b0997 JW0982 | periplasmic protein (TAT through IM), Trimethylamine-N-oxide reductase 1 TorA | Periplasmic | TAT secretion system. Trimethylamine N-oxide (TMAO) respiration system is encoded by the torCAD operon and comprises a periplasmic TMAO reductase (TorA) and a c-type cytochrome (TorC), which shuttles electrons to TorA. Interaction studies showed that, when immature, TorC can no longer bind TorA efficiently but can bind the periplasmic detector region of sensor TorS. Electron transfer and binding of the c-type cytochrome TorC to the trimethylamine N-oxide reductase TorD, a cytoplasmic chaperone that interacts with the unfolded trimethylamine N-oxide reductase enzyme (TorA). The absence of TorD protein leads to a two times decrease of the final amount of TorA enzyme. PMID: 15073303 | PMID: 11056172 PMID: 15073303 PMID: 17218314 PMID: 2512991 PMID: 9632735 | Periplasmic | G | N/A | Periplasm | Periplasmic (Experimental) | TAT | yes | yes | N | 40 | 0.294 | SpI | 39 | 1.32729 | - | 0 | 0 | Y | n14-24c42/43o | Periplasmic | 10 | 8 | ||||||||
| FLGK_ECOLI | P33235 | flgK flaS flaW | flaS flaW | b1082 JW1069 | Flagellar extra-cellular peripheral OM protein (Flagellum secreted), hook-associated 1 FlgK | Peripheral outer membrane protein facing the extra-cellular space | Part of FlgN-FlgK-FliJ-FlhA complex. First hook-filament junction protein. Flagella proteins contribute to the production of outer membrane vesicles. Analysis of E. coli W3110 OMVs revealed that they contain abundant levels of FliC. Compared to the parental strain expressing wild-type flgK, an E. coli strain with a flgK-null mutation produced reduced amounts of OMVs. PMID: 12730325 | PMID: 12631703 PMID: 12730325 PMID: 22233518 PMID: 24134841 | Peripheral outer membrane protein facing the extra-cellular space | F4 | N/A | Secreted | Cell surface appendage (Experimental) | flagellum | Flagellum | Fla | yes | yes | N | 18 | 0.238 | SpI | 17 | 2.83622 | - | 0 | 0 | N | i | Extracellular | 9 | 43 | |||||
| YEHA_ECOLI | P33340 | yehA | b2108 JW2095 | Fimbrial periplasmic protein (SEC through IM), YehA | Periplasmic | Putative chaperone???usher fimbrial operon yeh (class ??4); Unkown function. | PMID: 16372265 | Periplasmic | G | N/A | Periplasmic | fimbriae | SEC | no | yes | Y | 21 | 0.622 | SpI | 20 | 7.58765 | - | 0 | 0 | Y | n7-15c20/21o | Unknown | 2 | 48 | ||||||||
| YEHB_ECOLI | P33341 | yehB | b2109 JW2096 | Fimbrial OM b-barrel protein (SEC through IM, inserted in OM by BAM), YehB | Outer Membrane b-barrel protein | Putative chaperone???usher fimbrial operon yeh (class ??4); Usher. Disulfide bridge (809 825) | PMID: 16372265 | Outer Membrane b-barrel protein | H | N/A | Cell outer membrane | Outer Membrane ??-barrel protein | fimbriae | SEC | BAM | no | yes | Y | 23 | 0.679 | SpI | 22 | 11.7505 | - | 0 | 0 | Y | n7-18c22/23o | OuterMembrane | 10 | 5 | ||||||
| YEHC_ECOLI | P33342 | yehC | b2110 JW2097 | Fimbrial periplasmic protein (SEC through IM), Probable chaperone YehC | Periplasmic | Putative chaperone???usher fimbrial operon yeh (class ??4); Chaperone. Potential substrate of periplasmic peptidyl prolyl cis???trans isomerase SurA; sequence contains known binding motif of SurA [RYEF]X[RYEF][P]. | PMID: 18498364 | Periplasmic | G | N/A | Periplasm | Periplasmic | fimbriae | SEC | SURA | no | yes | N | 34 | 0.721 | SpI | 33 | 9.53077 | - | 1 | 0 | Y | n18-29c33/34o | Periplasmic | 10 | 43 | ||||||
| YEHD_ECOLI | P33343 | yehD | b2111 JW2098 | Fimbrial periplasmic protein (SEC through IM), YehD | Periplasmic | Putative chaperone???usher fimbrial operon yeh (class ??4); Fimbrial Subunit. Disulfide bond-containing protein PMID: 25664544 | PMID: 19820722 PMID: 20345943 PMID: 25664544 | Periplasmic | G | N/A | Periplasmic | fimbriae | SEC | no | yes | Y | 23 | 0.891 | SpI | 22 | 24.5442 | - | 1 | 0 | Y | n4-15c22/23o | Unknown | 2 | 95 | ||||||||
| YEHE_ECOLI | P33344 | yehE | b2112 JW2099 | periplasmic protein (SEC through IM), YehE | Periplasmic | Yeh operon is homolog to fim operon (yehd is fimI homolog). | PMID: 20345943 | Periplasmic | G | N/A | Periplasmic | SEC | no | yes | Y | 23 | 0.529 | SpI | 22 | 11.8793 | - | 0 | 0 | Y | n6-17c22/23o | CytoplasmicMembrane | 9 | 49 | |||||||||
| YEHR_ECOLI | P33354 | yehR | b2123 JW5351 | OM lipoprotein (SEC through IM, inserted in OM by LOL), YehR | Outer Membrane Lipoprotein | Proven pignal peptidase II substrate. PMID: 15174130 | PMID: 15174130 | Outer Membrane Lipoprotein | I | N/A | Cell membrane | Outer membrane Lipoprotein (Experimental) | SEC | LOL | no | yes | Y | 22 | 0.219 | SpII | 22 | 22.3044 | Pos+2=G | 0 | 0 | Y | n7-18c23/24o | Unknown | 2 | 81 | |||||||
| YEHZ_ECOLI | P33362 | yehZ osmF | osmF | b2131 JW2119 | periplasmic protein (SEC through IM), Glycine betaine-binding YehZ | Periplasmic | PMID: 15911532 | Periplasmic | G | N/A | Periplasm | Periplasmic (Experimental) | SEC | SURA | yes | yes | Y | 24 | 0.77 | SpI | 23 | 13.9464 | - | 0 | 0 | Y | n6-17c23/24o | CytoplasmicMembrane | 9 | 72 | |||||||
| BGLX_ECOLI | P33363 | bglX yohA | yohA | b2132 JW2120 | periplasmic protein (SEC through IM), beta-glucosidase BglX | Periplasmic | PMID: 8757730 | Periplasmic | G | Soluble IM Fraction | Periplasm | Periplasmic (Experimental) | SEC | yes | yes | Y | 21 | 0.824 | SpI | 20 | 6.29436 | - | 0 | 0 | Y | n4-15c20/21o | Periplasmic | 10 | 31 | ||||||||
| MDTQ_ECOLI | P33369 | mdtQ yohG yohH | yohG yohH | b2139 JW5838/JW5358 b2138 | OM lipoprotein (SEC through IM), inserted in OM by LOL), Putative multidrug resistance MdtQ | Outer Membrane Lipoprotein | I | N/A | Cell outer membrane | Cytoplasmic | SEC | LOL | pseudogene (Ochman & Davalos, 2006) | no | yes | Y | 24 | 0.204 | SpII | 21 | 10.0112 | Pos+2=A | 0 | 0 | Y | n9-20c38/39o | OuterMembrane | 10 | 2 | ||||||||
| PPDA_ECOLI | P33554 | ppdA | b2826 JW2794 | periplasmic protein (SEC through IM), Prepilin peptidase-dependent A PpdA | Periplasmic | G | N/A | Periplasmic | SEC | yes | yes | N | 27 | 0.172 | TMH | 0 | 7.10154 | - | 1 | 1 | N | i12-33o | CytoplasmicMembrane | 9 | 10 | ||||||||||||
| NIKA_ECOLI | P33590 | nikA | b3476 JW3441 | periplasmic protein (SEC through IM), Nickel-binding periplasmic protein NikA | Periplasmic | Might be is co-translationally translocated through Sec translocon. PMID: 7867647 Potential substrate for the SRP pathway. When fused to cytoplasmic protein thioredoxin promotes signal recognition particle-dependent translocation PMID: 15838024 | PMID: 15838024 PMID: 7867647 | Periplasmic | G | N/A | Periplasm | Periplasmic (Experimental) | SRP (exp) | SEC | yes | yes | Y | 23 | 0.82 | SpI | 22 | 20.282 | - | 0 | 0 | Y | n8-18c22/23o | Periplasmic | 10 | ||||||||
| YDBA_ECOLI | P33666 | ydbA | b4492 JW5802/JW1402 b1401/b1405 | OM b-barrel protein (SEC through IM), T5S secreted through OM), Putative exported YdbA | Outer Membrane b-barrel protein | Ag43 homologue; known autotransporter protein. Contains autotransporter beta-domain <a href="http://www.ebi.ac.uk/interpro/entry/IPR005546/proteins-matched?species=83333">(IPR005546)</a> | PMID: 15590781 | Outer Membrane b-barrel protein | H | IM (predicted) | Secreted | Cytoplasmic | SEC | Autotransporter(T5S) | pseudogene (Uniprot) | no | yes | Y | 22 | 0.75 | SpI | 20 | 0 | 21 | 0 | 0 | Y | n5-16c21/22o | Unknown | 6 | 28 | ||||||
| YBBC_ECOLI | P33668 | ybbC | b0498 JW0487 | OM lipoprotein (SEC through IM, inserted in OM by LOL), YbbC | Outer Membrane Lipoprotein | I | N/A | Outer membrane Lipoprotein | SEC | LOL | no | yes | Y | 18 | 0.367 | SpII | 17 | 8.97285 | Pos+2=N | 0 | 0 | Y | n6-17c25/26o | Unknown | 2 | 48 | |||||||||||
| YEJA_ECOLI | P33913 | yejA | b2177 JW2165 | periplasmic protein (SEC through IM), YejA | Periplasmic | Potential substrate of periplasmic peptidyl prolyl cis???trans isomerase SurA; sequence contains known binding motif of SurA [RYEF]X[RYEF][P]. | PMID: 18498364 | Periplasmic | G | Soluble IM Fraction | Periplasmic | SEC | SURA | no | yes | Y | 20 | 0.634 | SpI | 19 | 9.78276 | - | 1 | 0 | Y | n5-15c19/20o | Periplasmic | 10 | 19 | ||||||||
| YEJO_ECOLI | P33924 | yejO | b2190 JW5839 | OM b-barrel protein (SEC through IM), T5S secreted through OM), Putative YejO | Outer Membrane b-barrel protein | Ag43 homologue; predicted autotransporter. Contains autotransporter beta-domain <a href="http://www.ebi.ac.uk/interpro/entry/IPR005546/proteins-matched?species=83333">(IPR005546)</a> | PMID: 15590781 | Outer Membrane b-barrel protein | H | N/A | Cell outer membrane | cytoplasmic | SEC | Autotransporter(T5S) | Insertion Sequence, Insertion Element, pseudogene (Uniprot) | no | no | Y | 30 | 0.756 | SpI | 29 | 7.15031 | - | 0 | 0 | Y | n12-22c29/30o | Unknown | 7 | 29 | ||||||
| NAPA_ECOLI | P33937 | napA yojC yojD yojE | yojC yojD yojE | b2206 JW2194 | periplasmic protein (TAT through IM), nitrate reductase NapA | Periplasmic | TAT secretion system. Nitrate reductase, napFDAGHBC, is located in the periplasm. E. coli also expresses two membrane-bound proton-translocating nitrate reductases, encoded by the narGHJI and narZYWV operons. PMID: 11844760 PMID: 10234835 Disulfide bond-containing protein PMID: 25664544 | PMID: 10234835 PMID: 11844760 PMID: 17218314 PMID: 25664544 | Periplasmic | G | N/A | Periplasm | Periplasmic (Experimental) | TAT | yes | yes | Y | 32 | 0.617 | SpI | 31 | 20.6365 | - | 0 | 0 | Y | n13-23c31/32o | Periplasmic | 10 | ||||||||
| HOFQ_ECOLI | P34749 | hofQ hopQ | hopQ | b3391 JW3354 | OM b-barrel protein (SEC through IM, inserted in OM by BAM), DNA utilization HofQ | Outer Membrane b-barrel protein | Secretin family, homologous to the secretin of T2S (P45758 (GSPD_ECOLI)); homologus to ComE of H.influenza involved in DNA uptake | PMID: 16707682 | Outer Membrane b-barrel protein | H | N/A | Cell outer membrane | Periplasmic | SEC | BAM | yes | yes | Y | 19 | 0.736 | SpI | 19 | 10.1451 | - | 0 | 0 | Y | n5-13c18/19o | OuterMembrane | 10 | 63 | ||||||
| AMIA_ECOLI | P36548 | amiA yfeE | yfeE | b2435 JW2428 | periplasmic protein (TAT-SEC through IM), N-acetylmuramoyl-L-alanine amidase AmiA | Periplasmic | The Escherichia coli amidase AmiC is a periplasmic septal ring component exported via the twin-arginine transport pathwayPMID: 17218314PMID: 12787347. PMID: 12787347 | PMID: 12787347 PMID: 17218314 | Periplasmic | G | N/A | Periplasm | Periplasmic (Experimental) | TAT,SEC | yes | yes | Y | 35 | 0.684 | SpI | 34 | 15.9519 | - | 0 | 0 | Y | n16-26c34/35o | Unknown | 2 | 36 | |||||||
| ASR_ECOLI | P36560 | asr | b1597 JW5826 | periplasmic protein (SEC through IM), Acid shock protein Asr | Periplasmic | PMID: 12670971 | Periplasmic | G | N/A | Periplasm | Periplasmic (Experimental) | SEC | no | yes | Y | 22 | 0.907 | SpI | 21 | 28.9272 | - | 0 | 0 | Y | n4-15c21/22o | Periplasmic | 10 | ||||||||||
| CUEO_ECOLI | P36649 | cueO yacK | yacK | b0123 JW0119 | periplasmic protein (TAT-SEC through IM), Blue copper oxidase CueO | Periplasmic | TAT secretion system. PMID: 9298646 | PMID: 17218314 PMID: 9298646 | Periplasmic | G | N/A | Periplasm | Periplasmic (Experimental) | TAT,SEC | yes | yes | Y | 29 | 0.821 | SpI | 28 | 16.1248 | - | 0 | 0 | Y | n11-22c28/29o | Periplasmic | 10 | 6 | |||||||
| YACH_ECOLI | P36682 | yacH | b0117 JW0113 | periplasmic protein (SEC through IM), YacH | Periplasmic | G | N/A | Periplasmic | SEC | yes | yes | Y | 23 | 0.224 | SpI | 29 | 9.50022 | - | 0 | 0 | Y | n11-21c26/27o | Extracellular | 9 | 13 | ||||||||||||
| EAEH_ECOLI | P36943 | eaeH | b0297 JW0291 | periplasmic protein (SEC through IM), Putative attaching and effacing homolog EaeH | Periplasmic | Uniprot gives portential signal peptide (1-25) although it is not predicted by any tool. Intimin homologue but missing the C-terminus. Intimin is in the OM. Could be periplasmic. The signal peptide could be long autotransporter-like signal peptide. Intimin does not have a classical signal peptide either. | Periplasmic | G | N/A | Membrane anchored | SEC | pseudogene (Uniprot), pseudogene (Ochman & Davalos, 2006) | no | no | N | 45 | 0.383 | CYT | 0 | -0.200913 | - | 1 | 1 | N | i21-44o | OuterMembrane | 9 | 5 | |||||||||
| PAGP_ECOLI | P37001 | pagP crcA ybeG | crcA ybeG | b0622 JW0617 | OM b-barrel protein (SEC through IM, inserted in OM by BAM), Lipid A palmitoyltransferase PagP | Outer Membrane b-barrel protein | PMID: 11013210 PMID: 11266632 PMID: 12357033 | Outer Membrane b-barrel protein | H | N/A | Cell outer membrane | Outer Membrane ??-barrel protein (Experimental) | SEC | BAM | no | yes | N | 26 | 0.361 | CYT | 0 | -0.200913 | - | 0 | 0 | Y | n7-18c25/26o | OuterMembrane | 10 | 9 | |||||||
| YADK_ECOLI | P37016 | yadK | b0136 JW0132 | Fimbrial periplasmic protein (SEC through IM), YadK | Periplasmic | Putative chaperone???usher fimbrial operon (class ??4); Fimbrial Subunit. | PMID: 20345943 | Periplasmic | G | N/A | Fimbrium | Periplasmic | fimbriae | SEC | yes | yes | Y | 29 | 0.766 | SpI | 28 | 16.7043 | - | 1 | 0 | Y | n12-23c28/29o | Unknown | 2 | 41 | |||||||
| YADL_ECOLI | P37017 | yadL | b0137 JW0133 | Fimbrial periplasmic protein (SEC through IM), YadL | Periplasmic | Putative chaperone???usher fimbrial operon (class ??4); Unkown function. | PMID: 20345943 | Periplasmic | G | N/A | Fimbrium | Periplasmic | fimbriae | SEC | no | yes | Y | 29 | 0.815 | SpI | 28 | 19.3349 | - | 1 | 0 | Y | n11-22c28/29o | Unknown | 2 | 23 | |||||||
| YADM_ECOLI | P37018 | yadM | b0138 JW0134 | Fimbrial periplasmic protein (SEC through IM), YadM | Periplasmic | Putative chaperone???usher fimbrial operon (class ??4); Fimbrial Subunit. | PMID: 20345943 | Periplasmic | G | N/A | Fimbrium | Periplasmic | fimbriae | SEC | no | yes | Y | 24 | 0.878 | SpI | 23 | 14.8521 | - | 0 | 0 | Y | n9-17c23/24o | Unknown | 2 | 25 | |||||||
| BTUF_ECOLI | P37028 | btuF yadT | yadT | b0158 JW0154 | periplasmic protein (SEC through IM), Vitamin B12-binding protein BtuF | Periplasmic | Disulfide bridge (183 259) Disulfide bond-containing protein PMID: 25664544 | PMID: 11790740 PMID: 25664544 | Periplasmic | G | N/A | Periplasm | Periplasmic (Experimental) | SEC | yes | yes | Y | 23 | 0.751 | SpI | 22 | 10.8439 | - | 0 | 0 | Y | n8-18c22/23o | Periplasmic | 10 | ||||||||
| YAEI_ECOLI | P37049 | yaeI | b0164 JW5014 | periplasmic protein (SEC through IM), Phosphodiesterase YaeI | Periplasmic | G | N/A | Membrane anchored | SEC | no | yes | N | 47 | 0.129 | CYT | 0 | -0.200913 | - | 0 | 0 | Y | n7-17c22/23o | Unknown | 2 | 6 | ||||||||||||
| YADN_ECOLI | P37050 | yadN | b0141 JW0137 | Fimbrial periplasmic protein (SEC through IM), YadN | Periplasmic | Putative chaperone???usher fimbrial operon (class ??4); Fimbrial Subunit. Disulfide bridge (41 86) | PMID: 19820722 PMID: 20345943 | Periplasmic | G | N/A | Fimbrium | Periplasmic | fimbriae | SEC | CU | no | yes | Y | 24 | 0.713 | SpI | 23 | 17.9817 | - | 0 | 0 | Y | n5-15c23/24o | Extracellular | 9 | 77 | ||||||
| YAEF_ECOLI | P37056 | yaeF yaeK | yaeK | b0193 JW5016 | OM lipoprotein (SEC through IM), inserted in OM by LOL), Probable peptidase YaeF | Outer Membrane Lipoprotein | I | N/A | Cell inner membrane | Outer membrane Lipoprotein | SEC | LOL | no | yes | Y | 26 | 0.176 | SpII | 20 | 9.80359 | Pos+2=T | 0 | 0 | Y | n10-21c26/27o | Unknown | 2 | 50 | |||||||||
| SLP_ECOLI | P37194 | slp | b3506 JW3474 | OM lipoprotein (SEC through IM, inserted in OM by LOL), Slp | Outer Membrane Lipoprotein | Proven pignal peptidase II substrate. Outer membrane protein determined by 2D gel electrophoresis (Lopez-Campistrous et al 2005, Molloy et al 2000) Identified in nucleoids collected by sucrose gradient centrifugation and analyzed by liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS). PMID: 21541338 PMID: 10806384 PMID: 9298646 | PMID: 10806384 PMID: 15174130 PMID: 15911532 PMID: 16079137 PMID: 20932056 PMID: 21541338 PMID: 9298646 | Outer Membrane Lipoprotein | I | Soluble IM Fraction | Cell outer membrane | Outer membrane Lipoprotein (Experimental) | SEC | LOL | yes | yes | Y | 18 | 0.153 | SpII | 18 | 21.4364 | Pos+2=S | 0 | 0 | Y | n6-16c21/22o | OuterMembrane | 10 | ||||||||
| YHJA_ECOLI | P37197 | yhjA | b3518 JW3486 | IM lipoprotein (SEC through IM), inserted in OM by LOL), Probable cytochrome c peroxidase YhjA | Inner Membrane Lipoprotein | By virtue of its homology to the Neisseria protein (Q5F5Z9_neigo1) | PMID: 12720546 | Inner Membrane Lipoprotein | E | N/A | Membrane anchored | SEC | LOL | no | yes | N | 29 | 0.287 | SpI | 28 | 4.74738 | - | 1 | 1 | N | i7-28o | Periplasmic | 9 | 21 | ||||||||
| YBCH_ECOLI | P37325 | ybcH | b0567 JW0556 | periplasmic protein (SEC through IM), YbcH | Periplasmic | G | N/A | Periplasmic | SEC | no | yes | Y | 21 | 0.6 | SpI | 20 | 14.6445 | - | 1 | 0 | Y | n4-15c20/21o | CytoplasmicMembrane | 9 | 26 | ||||||||||||
| MODA_ECOLI | P37329 | modA | b0763 JW0746 | periplasmic protein (SEC through IM), Molybdate-binding ModA | Periplasmic | PMID: 8576221 | Periplasmic | G | N/A | Periplasm | Periplasmic (Experimental) | SEC | no | yes | Y | 25 | 0.754 | SpI | 24 | 18.4352 | - | 0 | 0 | Y | n8-19c24/25o | Periplasmic | 10 | 99 | |||||||||
| XYLF_ECOLI | P37387 | xylF xylT | xylT | b3566 JW3538 | periplasmic protein (SEC through IM), D-xylose-binding periplasmic protein XylF | Periplasmic | PMID: 9298646 | Periplasmic | G | N/A | Periplasm | Periplasmic (Experimental) | SEC | yes | yes | Y | 24 | 0.78 | SpI | 23 | 14.4657 | - | 0 | 0 | Y | n6-17c23/24o | Periplasmic | 10 | |||||||||
| MDTE_ECOLI | P37636 | mdtE yhiU | yhiU | b3513 JW3481 | OM lipoprotein (SEC through IM), inserted in OM by LOL), Multidrug resistance MdtE | Outer Membrane Lipoprotein | Outer membrane protein related to resistance to ampicillin and tetracycline Proven pignal peptidase II substrate. | PMID: 15174130 PMID: 16372265 | Outer Membrane Lipoprotein | I | Soluble IM Fraction | Cell inner membrane | Inner membrane lipoprotein | SEC | LOL | yes | yes | Y | 26 | 0.181 | SpII | 20 | 22.544 | Pos+2=D | 0 | 0 | Y | n7-15c20/21o | CytoplasmicMembrane | 9 | 89 | ||||||
| YHJJ_ECOLI | P37648 | yhjJ | b3527 JW3495 | periplasmic protein (SEC through IM), YhjJ | Periplasmic | Potential Skp substrate PMID: 19953543 PMID: 9298646 Disulfide bond-containing protein PMID: 25664544 | PMID: 19953543 PMID: 25664544 PMID: 9298646 | Periplasmic | G | N/A | Periplasm | Periplasmic (Experimental) | SEC | SKP | yes | yes | Y | 25 | 0.722 | SpI | 24 | 14.0569 | - | 0 | 0 | Y | n8-19c24/25o | Unknown | 2 | 83 | |||||||
| BCSC_ECOLI | P37650 | bcsC yhjL | yhjL | b3530 JW5942 | periplasmic protein (SEC through IM), Cellulose synthase operon C BcsC | Periplasmic | G | N/A | Periplasmic | SEC | no | yes | Y | 24 | 0.51 | SpI | 23 | 6.58448 | - | 0 | 0 | Y | n8-18c23/24o | Unknown | 4 | ||||||||||||
| GUN_ECOLI | P37651 | bcsZ bcsC yhjM | bcsC yhjM | b3531 JW3499 | periplasmic protein (SEC through IM), Endoglucanase BcsZ | Periplasmic | PMID: 10102357 PMID: 11260463 PMID: 21454578 PMID: 24097954 | Periplasmic | G | N/A | Secreted | Periplasmic (Experimental) | SEC | no | yes | Y | 22 | 0.88 | SpII | 22 | 12.5942 | Pos+2=T | 0 | 0 | Y | n6-17c21/22o | Extracellular | 10 | 6 | ||||||||
| YHJY_ECOLI | P37663 | yhjY | b3548 JW5659 | OM b-barrel protein (SEC through IM, T5S secreted through OM), YhjY | Outer Membrane b-barrel protein | Ag43 homologue ; predicted autotransporter. Contains autotransporter beta-domain <a href="http://www.ebi.ac.uk/interpro/entry/IPR005546/proteins-matched?species=83333">(IPR005546)</a> | PMID: 15590781 | Outer Membrane b-barrel protein | H | N/A | Periplasmic | SEC | Autotransporter(T5S) | no | yes | N | 31 | 0.371 | SpI | 30 | 11.8549 | - | 1 | 0 | Y | n14-25c30/31o | Unknown | 7 | 9 | ||||||||
| YIAD_ECOLI | P37665 | yiaD | b3552 JW5657 | OM lipoprotein (SEC through IM), inserted in OM by LOL), Probable YiaD | Outer Membrane Lipoprotein | Proven pignal peptidase II substrate. PMID: 15174130 | PMID: 15174130 PMID: 15919996 PMID: 20932056 | Outer Membrane Lipoprotein | I | out/out | N/A | Cell inner membrane | Outer membrane Lipoprotein (Experimental) | SEC | LOL | yes | yes | Y | 18 | 0.162 | SpII | 20 | 21.9879 | Pos+2=T | 3 | 2 | Y | n5-16c20/21o36-55i62-81o | OuterMembrane | 10 | 63 | ||||||
| YIAO_ECOLI | P37676 | yiaO | b3579 JW3551 | periplasmic protein (SEC through IM), 2,3-diketo-L-gulonate-binding periplasmic YiaO | Periplasmic | PMID: 16385129 | Periplasmic | G | N/A | Periplasm | Periplasmic | SEC | no | yes | Y | 25 | 0.815 | SpI | 24 | 15.7433 | - | 0 | 0 | Y | n6-17c24/25o | Periplasmic | 10 | 20 | |||||||||
| YIAT_ECOLI | P37681 | yiaT | b3584 JW3556 | OM b-barrel protein (SEC through IM), Putative YiaT | Outer Membrane b-barrel protein | PMID: 20639525 | Outer Membrane b-barrel protein | H | N/A | Cell outer membrane | Outer Membrane ??-barrel protein | SEC | no | yes | Y | 22 | 0.602 | SpI | 21 | 11.315 | - | 0 | 0 | Y | n7-17c21/22o | OuterMembrane | 10 | 22 | |||||||||
| ENVC_ECOLI | P37690 | envC yibP | yibP | b3613 JW5646 | periplasmic protein (SEC through IM), Murein hydrolase activator EnvC | Periplasmic | PMID: 12113939 PMID: 15165230 PMID: 19525345 PMID: 8472900 | Periplasmic | G | Soluble IM Fraction | Periplasm | Inner Membrane Lipoprotein (Experimental) | SEC | yes | yes | Y | 35 | 0.862 | SpI | 34 | 13.522 | - | 1 | 0 | Y | n18-29c34/35o | OuterMembrane | 9 | 10 | ||||||||
| YIBQ_ECOLI | P37691 | yibQ | b3614 JW5645 | periplasmic protein (SEC through IM), YibQ | Periplasmic | PMID: 19953543 | Periplasmic | G | N/A | Periplasmic | SEC | yes | yes | Y | 24 | 0.782 | SpI | 23 | 13.9145 | - | 1 | 0 | Y | n8-18c23/24o | Unknown | 2 | 14 | ||||||||||
| RMLA1_ECOLI | P37744 | rfbA rmlA rmlA1 | rmlA rmlA1 | b2039 JW2024 | periplasmic protein (SEC through IM), Glucose-1-phosphate thymidylyltransferase 1 RfbA | Periplasmic | PMID: 23138692 PMID: 7537247 | Periplasmic | G | IM (predicted-detected) | Cytoplasmic | SEC | yes | yes | N | 24 | 0.125 | CYT | 0 | -0.200913 | - | 0 | 0 | Y | n7-18c23/24o | Cytoplasmic | 9 | 63 | |||||||||
| GLTI_ECOLI | P37902 | gltI ybeJ yzzK | ybeJ yzzK | b0655 JW5092 | periplasmic protein (SEC through IM), Glutamate/aspartate import solute-binding protein GltI | Periplasmic | Substrate of periplasmic disulfide oxidoreductases DsbA. Identified DsbA substrate PMID: 19953543 PMID: 8740179 Potential substrate for the SRP pathway. It was found to be down-regulated in the periplasmic compartment of SRP-depleted cells. PMID: 22030350 Disulfide bond-containing protein PMID: 25664544 | PMID: 14726535 PMID: 19953543 PMID: 22030350 PMID: 25664544 PMID: 8740179 | Periplasmic | G | N/A | Periplasm | Periplasmic (Experimental) | SEC | DSBA | yes | yes | Y | 23 | 0.775 | SpI | 22 | 21.5903 | - | 0 | 0 | Y | n7-17c22/23o | Periplasmic | 10 | 93 | ||||||
| YBGD_ECOLI | P37909 | ybgD | b0719 JW0709 | Fimbrial periplasmic protein (SEC through IM), YbgD | Periplasmic | Putative chaperone???usher fimbrial operon yeh (class ??); Fimbrial Subunit. Disulfide bridge (42 81) | PMID: 19820722 PMID: 20345943 | Periplasmic | G | N/A | Fimbrium | Periplasmic | fimbriae | SEC | CU | no | yes | Y | 24 | 0.878 | SpI | 23 | 15.4399 | - | 0 | 0 | Y | n7-18c23/24o | Extracellular | 9 | |||||||
| SFMF_ECOLI | P38052 | sfmF ybcG | ybcG | b0534 JW5072 | Fimbrial periplasmic protein (SEC through IM), SfmF | Periplasmic | Potential fimbrial sfm operon (class ??1); Fimbrial Subunit. Disulfide bridge (40 80) | PMID: 19820722 PMID: 20345943 | Periplasmic | G | N/A | Fimbrium | Periplasmic | fimbriae | SEC | CU | no | yes | Y | 21 | 0.763 | SpI | 20 | 16.3129 | - | 0 | 0 | Y | n4-15c20/21o | Extracellular | 9 | 64 | |||||
| TORT_ECOLI | P38683 | torT yccH | yccH | b0994 JW0979 | periplasmic protein (SEC through IM), Periplasmic TorT | Periplasmic | Might be is co-translationally translocated through Sec translocon. PMID: 8576063 The precursor of TorT harbouring hydrophobic signal sequence require SRP and FtsY in addition to SecA for maximal translocation. PMID: 24717922 Potential substrate for the SRP pathway. When fused to cytoplasmic protein thioredoxin promotes signal recognition particle-dependent translocation PMID: 15838024 Disulfide bond-containing protein PMID: 25664544 | PMID: 15838024 PMID: 24717922 PMID: 25664544 PMID: 8576063 | Periplasmic | G | N/A | Periplasm | Periplasmic (Experimental) | SRP (exp) | SEC | yes | yes | Y | 19 | 0.789 | SpI | 18 | 12.9296 | - | 0 | 0 | Y | n3-14c18/19o | Periplasmic | 10 | 12 | ||||||
| DEGQ_ECOLI | P39099 | degQ hhoA | hhoA | b3234 JW3203 | periplasmic protein (SEC through IM), pH-dependent serine endoprotease DegQ | Periplasmic | PMID: 8576051 PMID: 8576052 PMID: 9740056 | Periplasmic | G | N/A | Periplasm | Periplasmic (Experimental) | SEC | yes | yes | Y | 28 | 0.555 | SpI | 27 | 17.6659 | - | 1 | 0 | Y | n7-19c27/28o | Periplasmic | 10 | 66 | ||||||||
| YCHO_ECOLI | P39165 | ychO ychP | ychP | b1220 JW1211 | periplasmic protein (SEC through IM), YchO | Homologous to intimin / invasin family (yeej_coli). | Periplasmic | G | N/A | Periplasm | Cytoplasmic | SEC | yes | yes | Y | 25 | 0.55 | SpI | 24 | 8.3661 | - | 0 | 0 | Y | n8-19c24/25o | OuterMembrane | 10 | 7 | |||||||||
| ZNUA_ECOLI | P39172 | znuA yebL yzzP | yebL yzzP | b1857 JW5831 | periplasmic protein (SEC through IM), High-affinity zinc uptake system ZnuA | Periplasmic | Disulfide bond-containing protein PMID: 25664544 | PMID: 25664544 PMID: 9298646 | Periplasmic | G | N/A | Periplasm | Periplasmic (Experimental) | SEC | yes | yes | Y | 27 | 0.573 | SpI | 23 | 18.3411 | - | 0 | 0 | Y | n6-17c26/27o | Periplasmic | 10 | 84 | |||||||
| ERFK_ECOLI | P39176 | erfK yeeG yzzT | yeeG yzzT | b1990 JW1968 | periplasmic peripheral OM protein (SEC through IM), Probable L,D-transpeptidase ErfK | Peripheral outer membrane protein facing the periplasm | Potential substrate of periplasmic peptidyl prolyl cis???trans isomerase SurA; sequence contains known binding motif of SurA [RYEF]X[RYEF][P]. ErfK has been shown to crosslink Braun's lipoprotein (Lpp) to the peptidoglycan. PMID: 9298646 | PMID: 18498364 PMID: 23832002 PMID: 9298646 | Peripheral outer membrane protein facing the periplasm | F3 | N/A | Periplasm | Periplasmic (Experimental) | SEC | SURA | yes | yes | Y | 22 | 0.658 | SpI | 21 | 17.0042 | - | 0 | 0 | Y | n6-16c21/22o | Unknown | 2 | 11 | ||||||
| AG43_ECOLI | P39180 | flu yeeQ yzzX | yeeQ yzzX | b2000 JW1982 | OM b-barrel protein (SEC through IM), T5S and TAM secreted in OM), Antigen 43 Flu | Outer Membrane b-barrel protein | Outer membrane protein determined by 2D gel electrophoresis (PMID: 15911532, PMID: 12624733, PMID: 10806384). Potential substrate of TAM secretion systemPMID: 22466966. Contains autotransporter beta-domain <a href="http://www.ebi.ac.uk/interpro/entry/IPR005546/proteins-matched?species=83333">(IPR005546)</a> PMID: 9298646 | PMID: 10806384 PMID: 12624733 PMID: 15590781 PMID: 15911532 PMID: 22466966 PMID: 22905239 PMID: 9298646 | Outer Membrane b-barrel protein | H | N/A | Antigen 43: Periplasm | Outer Membrane ??-barrel protein (Experimental) | SEC | Autotransporter(T5S), TAM | Prophage Integrase (EcoGene) | no | no | N | 53 | 0.646 | SpI | 52 | 5.41594 | - | 1 | 0 | Y | n34-45c52/53o | OuterMembrane | 10 | 32 | |||||
| YTFJ_ECOLI | P39187 | ytfJ | b4216 JW4175 | periplasmic protein (SEC through IM), YtfJ | Periplasmic | PMID: 9298646 | Periplasmic | G | N/A | Periplasm | Periplasmic(experimental) | SEC | yes | yes | Y | 21 | 0.734 | SpI | 20 | 13.6826 | - | 0 | 0 | Y | n6-14c22/23o | Unknown | 2 | 18 | |||||||||
| FIMI_ECOLI | P39264 | fimI | b4315 JW5779 | Fimbrial periplasmic protein (SEC through IM), Fimbrin-like FimI | Periplasmic | Fim operon (class ??1); Fimbrial Subunit. Required for the regulation of length and mediation of adhesion of Escherichia coli type 1 fimbriae. Disulfide bridge (43 83) | PMID: 19820722 PMID: 20345943 PMID: 2890081 | Periplasmic | G | N/A | Fimbrium | Periplasmic | fimbriae | SEC | CU | no | yes | Y | 20 | 0.716 | SpI | 19 | 16.0857 | - | 0 | 0 | Y | n6-13c19/20o | Extracellular | 9 | 37 | ||||||
| ALSB_ECOLI | P39265 | alsB yjcX | yjcX | b4088 JW4049 | periplasmic protein (SEC through IM), D-allose-binding periplasmic AlsB | Periplasmic | PMID: 14499611 | Periplasmic | G | N/A | Periplasm | Periplasmic (Experimental) | SEC | no | yes | Y | 24 | 0.81 | SpI | 23 | 18.079 | - | 1 | 0 | Y | n8-18c23/24o | Periplasmic | 10 | 53 | ||||||||
| BSMA_ECOLI | P39297 | bsmA yjfO | yjfO | b4189 JW5743 | OM lipoprotein (SEC through IM, inserted in OM by LOL), BsmA | Outer Membrane Lipoprotein | PMID: 20932056 | Outer Membrane Lipoprotein | I | N/A | Cell membrane | Outer membrane Lipoprotein | SEC | LOL | yes | yes | Y | 30 | 0.447 | SpII | 24 | 20.0908 | Pos+2=S | 1 | 0 | Y | n13-22c27/28o | Unknown | 2 | 46 | |||||||
| YTFQ_ECOLI | P39325 | ytfQ | b4227 JW4186 | periplasmic protein (SEC through IM), ABC transporter periplasmic-binding YtfQ | Periplasmic | Identified DsbA substrate PMID: 19953543 PMID: 9298646 Disulfide bond-containing protein PMID: 25664544 | PMID: 19953543 PMID: 25664544 PMID: 9298646 | Periplasmic | G | N/A | Periplasm | Periplasmic (Experimental) | SEC | DSBA | yes | yes | Y | 22 | 0.849 | SpI | 21 | 21.6548 | - | 1 | 0 | Y | n5-13c21/22o | Periplasmic | 10 | 90 | |||||||
| NANM_ECOLI | P39371 | nanM yjhT | yjhT | b4310 JW5777 | periplasmic protein (SEC through IM), N-acetylneuraminate epimerase NanM | Periplasmic | PMID: 18063573 | Periplasmic | G | N/A | Periplasm | Periplasmic | SEC | no | yes | Y | 20 | 0.747 | SpI | 19 | 18.4486 | - | 0 | 0 | Y | n4-15c19/20o | Unknown | 2 | 75 | ||||||||
| YGIL_ECOLI | P39834 | ygiL | b3043 JW3011 | Fimbrial periplasmic protein (SEC through IM), YgiL | Periplasmic | Putative chaperone???usher fimbrial operon (class ??); Unknown function. Disulfide bridge (41 80) | PMID: 19820722 PMID: 20345943 | Periplasmic | G | N/A | Fimbrium | Periplasmic | fimbriae | SEC | CU | no | yes | Y | 24 | 0.867 | SpI | 23 | 15.5994 | - | 0 | 0 | Y | n7-18c23/24o | Extracellular | 9 | 92 | ||||||
| OPGD_ECOLI | P40120 | mdoD opgD ydcG yzzZ | opgD ydcG yzzZ | b1424 JW1420 | periplasmic protein (TAT-SEC through IM), Glucans biosynthesis D MdoD | Periplasmic | TAT secretion system. PMID: 9298646 Disulfide bond-containing protein PMID: 25664544 | PMID: 17218314 PMID: 25664544 PMID: 9298646 | Periplasmic | G | N/A | Periplasm | Periplasmic (Experimental) | TAT,SEC | no | yes | Y | 33 | 0.683 | SpI | 32 | 18.7932 | - | 0 | 0 | Y | n14-25c32/33o | Periplasmic | 10 | 61 | |||||||
| NLPE_ECOLI | P40710 | nlpE cutF | cutF | b0192 JW0188 | OM lipoprotein (SEC through IM, inserted in OM by LOL), NlpE | Outer Membrane Lipoprotein | Proven pignal peptidase II substrate. PMID: 7635807 | PMID: 15174130 PMID: 20932056 PMID: 7635807 | Outer Membrane Lipoprotein | I | N/A | Cell outer membrane | Outer membrane Lipoprotein(experimental) | SEC | LOL | yes | yes | Y | 36 | 0.226 | SpII | 20 | 12.8918 | Pos+2=N | 0 | 0 | Y | n6-17c35/36o | OuterMembrane | 10 | 112 | ||||||
| YCBF_ECOLI | P40876 | ycbF | b0944 JW5124 | Fimbrial periplasmic protein (SEC through IM), Chaperone YcbF | Periplasmic | Potential fimbrial ycb operon (class ??1); Chaperone. Potential substrate of periplasmic peptidyl prolyl cis???trans isomerase SurA; sequence contains known binding motif of SurA [RYEF]X[RYEF][P]. | PMID: 18498364 | Periplasmic | G | N/A | Periplasm | Periplasmic | fimbriae | SEC | SURA | no | yes | N | 27 | 0.646 | SpI | 26 | 9.39817 | - | 1 | 0 | Y | n8-19c26/27o | Periplasmic | 9 | 19 | ||||||
| MLTB_ECOLI | P41052 | mltB | b2701 JW2671 | OM lipoprotein (SEC through IM), inserted in OM by LOL), Membrane-bound lytic murein transglycosylase B MltB | Outer Membrane Lipoprotein | Proven pignal peptidase II substrate. PMID: 15174130 | PMID: 15174130 PMID: 20932056 | Outer Membrane Lipoprotein | I | Soluble IM Fraction | Cell outer membrane; Lipid-anchor; Periplasmic side | Outer membrane Lipoprotein (Experimental) | SEC | LOL | yes | yes | Y | 24 | 0.231 | SpII | 18 | 24.349 | Pos+2=S | 0 | 0 | Y | n7-17c21/22o | CytoplasmicMembrane | 9 | 27 | |||||||
| YGJJ_ECOLI | P42591 | ygjJ | b3079 JW3050 | periplasmic protein (SEC through IM), YgjJ | Periplasmic | Potential substrate of periplasmic peptidyl prolyl cis???trans isomerase SurA; sequence contains known binding motif of SurA [RYEF]X[RYEF][P]. | PMID: 18498364 | Periplasmic | G | N/A | Periplasmic | SEC | SURA | yes | yes | Y | 22 | 0.252 | SpI | 24 | 7.25386 | - | 0 | 0 | Y | n3-14c19/20o | Unknown | 2 | 31 | ||||||||
| YGJK_ECOLI | P42592 | ygjK | b3080 JW3051 | periplasmic protein (SEC through IM), Glucosidase YgjK | Periplasmic | G | N/A | Periplasmic | SEC | no | yes | Y | 23 | 0.898 | SpI | 22 | 21.1996 | - | 0 | 0 | Y | n5-16c25/26o | Periplasmic | 9 | 22 | ||||||||||||
| YQJC_ECOLI | P42616 | yqjC | b3097 JW5516 | periplasmic protein (SEC through IM), YqjC | Periplasmic | G | N/A | Periplasmic | SEC | yes | yes | Y | 21 | 0.508 | SpI | 20 | 9.52426 | - | 0 | 0 | Y | n5-15c20/21o | Unknown | 2 | 79 | ||||||||||||
| YRAH_ECOLI | P42913 | yraH | b3142 JW3111 | Fimbrial periplasmic protein (SEC through IM), YraH | Periplasmic | Potential fimbrial yra operon (class ??1); Fimbrial Subunit. Disulfide bridge (38 78) | PMID: 19820722 PMID: 20345943 | Periplasmic | G | N/A | Fimbrium | Periplasmic | fimbriae | SEC | CU | no | yes | Y | 23 | 0.673 | SpI | 22 | 11.7644 | - | 0 | 0 | Y | n7-17c22/23o | Extracellular | 9 | |||||||
| YRAI_ECOLI | P42914 | yraI | b3143 JW3112 | Fimbrial periplasmic protein (SEC through IM), Probable chaperone YraI | Periplasmic | Potential fimbrial yra operon (class ??1); Chaperone. Potential substrate of periplasmic peptidyl prolyl cis???trans isomerase SurA; sequence contains known binding motif of SurA [RYEF]X[RYEF][P]. Might be is co-translationally translocated through Sec translocon. Potential substrate for the SRP pathway. When fused to cytoplasmic protein thioredoxin promotes signal recognition particle-dependent translocation PMID: 15838024 | PMID: 15838024 PMID: 18498364 PMID: 20345943 | Periplasmic | G | N/A | Periplasm | Periplasmic | fimbriae | SRP (exp) | SEC | SURA | no | yes | Y | 24 | 0.709 | SpI | 23 | 14.1781 | - | 1 | 0 | Y | n7-18c23/24o | Periplasmic | 10 | 56 | |||||
| YRAJ_ECOLI | P42915 | yraJ | b3144 JW3113 | Fimbrial OM b-barrel protein (SEC through IM, inserted in OM by BAM), YraJ | Outer Membrane b-barrel protein | Potential fimbrial yra operon (class ??1); Usher. Disulfide bridge (815 837) | PMID: 20345943 | Outer Membrane b-barrel protein | H | N/A | Cell outer membrane | Outer Membrane ??-barrel protein | fimbriae | SEC | BAM | no | yes | N | 41 | 0.51 | SpI | 40 | 9.33083 | - | 0 | 0 | N | i | OuterMembrane | 10 | 6 | ||||||
| YRAK_ECOLI | P43319 | yraK | b3145 JW3114 | Fimbrial periplasmic protein (SEC through IM), YraK | Periplasmic | Potential fimbrial yra operon (class ??1); Potential tip-adhesin. Potential substrate of periplasmic peptidyl prolyl cis???trans isomerase SurA; sequence contains known binding motif of SurA [RYEF]X[RYEF][P]. | PMID: 18498364 PMID: 19820722 PMID: 20345943 | Periplasmic | G | N/A | Fimbrium | Periplasmic | fimbriae | SEC | SURA | CU | no | yes | Y | 21 | 0.726 | SpI | 20 | 18.7753 | - | 0 | 0 | Y | n4-15c20/21o | Unknown | 2 | 78 | |||||
| YCAL_ECOLI | P43674 | ycaL | b0909 JW0892 | OM lipoprotein (SEC through IM), inserted in OM by LOL), Metalloprotease YcaL | Outer Membrane Lipoprotein | Proven pignal peptidase II substrate. PMID: 15174130 | PMID: 15174130 | Outer Membrane Lipoprotein | I | N/A | Outer membrane Lipoprotein (Experimental) | SEC | LOL | yes | yes | N | 26 | 0.214 | SpII | 19 | 26.2238 | Pos+2=Q | 0 | 0 | Y | n6-17c25/26o | Unknown | 2 | 24 | ||||||||
| YHCD_ECOLI | P45420 | yhcD | b3216 JW3183 | Fimbrial OM b-barrel protein (SEC through IM, inserted in OM by BAM), YhcD | Outer Membrane b-barrel protein | Potential fimbrial yhc operon (class ??); Uknown function. Disulfide bridge (769 792) | PMID: 20345943 | Outer Membrane b-barrel protein | H | N/A | Cell outer membrane | Outer Membrane ??-barrel protein | fimbriae | SEC | BAM | no | yes | Y | 22 | 0.809 | SpI | 21 | 10.0887 | - | 0 | 0 | Y | n5-16c21/22o | OuterMembrane | 10 | |||||||
| YHCE_ECOLI | P45421 | yhcE | b4569 b3217 | Fimbrial periplasmic protein (SEC through IM), Putative YhcE | Periplasmic | Potential fimbrial yhc operon (class ??); Uknown function. | PMID: 20345943 | Periplasmic | G | N/A | Periplasmic | fimbriae | SEC | pseudogene (Uniprot) | no | no | Y | 20 | 0.69 | SpI | 19 | 4.02903 | - | 0 | 0 | Y | n4-11c16/17o | Unknown | 2 | 20 | |||||||
| YHCF_ECOLI | P45422 | yhcF | b3219 JW3188 | Fimbrial periplasmic protein (SEC through IM), YhcF | Periplasmic | Potential fimbrial yhc operon (class ??); Fimbrial Subunit. | PMID: 20345943 | Periplasmic | G | N/A | Periplasmic | fimbriae | SEC | no | yes | Y | 21 | 0.835 | SpI | 20 | 16.471 | - | 0 | 0 | Y | n6-16c20/21o | Unknown | 2 | 7 | ||||||||
| LPOA_ECOLI | P45464 | lpoA yraM | yraM | b3147 JW3116 | OM lipoprotein (SEC through IM), inserted in OM by LOL), Penicillin-binding activator LpoA | Outer Membrane Lipoprotein | The two OM lipoproteins, LpoA and LpoB, are essential for the function, respectively, of PBP1A and PBP1B, the major E. coli bifunctional peptidoglycans (PG) synthases. Each Lpo protein binds specifically to its cognate PBP and stimulates its transpeptidase. Outer membrane protein determined by 2D gel electrophoresis (Lopez-Campistrous et al 2005) Disulfide bond-containing protein PMID: 25664544 | PMID: 15911532 PMID: 20932056 PMID: 21183073 PMID: 21183074 PMID: 25664544 | Outer Membrane Lipoprotein | I | Soluble IM Fraction | Cell outer membrane | Membrane Lipoprotein | SEC | LOL | no | yes | Y | 26 | 0.162 | SpII | 26 | 12.6937 | Pos+2=G | 0 | 0 | Y | n14-27c45/46o | CytoplasmicMembrane | 9 | 105 | ||||||
| YFAL_ECOLI | P45508 | yfaL yfaF yfaJ yfaK | yfaF yfaJ yfaK | b2233 JW2227 | OM b-barrel protein (SEC through IM), T5S secreted through OM), Probable autotransporter YfaL | Outer Membrane b-barrel protein | Ag43 homologue; predicted autotransporter. Contains autotransporter beta-domain <a href="http://www.ebi.ac.uk/interpro/entry/IPR005546/proteins-matched?species=83333">(IPR005546)</a> | PMID: 15590781 | Outer Membrane b-barrel protein | H | N/A | Probable autotransporter YfaL: Periplasm; Probable secreted autotransporter protein YfaL: Secreted Cell surface; Probable autotransporter YfaL translocator: Cell outer membrane | Periplasmic | SEC | Autotransporter(T5S) | yes | yes | Y | 29 | 0.358 | SpI | 28 | 13.6585 | - | 0 | 0 | Y | n11-22c29/30o | OuterMembrane | 9 | 52 | ||||||
| FKBA_ECOLI | P45523 | fkpA yzzS | yzzS | b3347 JW3309 | periplasmic protein (SEC through IM), FKBP-type peptidyl-prolyl cis-trans isomerase FkpA | Periplasmic | SecB substrate. PMID: 9298646 Potential substrate for the SRP pathway. It was found to be down-regulated in the periplasm and up-regulated in the cytoplasm of SRP-depleted cells. PMID: 22030350 | PMID: 16352602 PMID: 22030350 PMID: 9298646 | Periplasmic | G | N/A | Periplasm | Periplasmic (Experimental) | SECB | SEC | yes | yes | Y | 26 | 0.701 | SpI | 25 | 14.7593 | - | 0 | 0 | Y | n7-18c25/26o | Periplasmic | 10 | |||||||
| YFEN_ECOLI | P45564 | yfeN | b2408 JW2399 | OM b-barrel protein (SEC through IM, inserted in OM by BAM), YfeN | Outer Membrane b-barrel protein | H | N/A | Outer Membrane ??-barrel protein | SEC | BAM | no | yes | Y | 23 | 0.452 | SpI | 22 | 12.5667 | - | 0 | 0 | Y | n5-16c22/23o | Unknown | 2 | 6 | |||||||||||
| AIS_ECOLI | P45565 | ais pmrG | pmrG | b2252 JW2246 | periplasmic protein (SEC through IM), polysaccharide core heptose Ais | Periplasmic | Periplasmic protein by virtue of its homology with the well characterized Salmonella homologue PmrGPMID: 16803591. Disulfide bond-containing protein PMID: 25664544 | PMID: 16803591 PMID: 25664544 | Periplasmic | G | N/A | Periplasm | Membrane anchored | SEC | yes | yes | N | 32 | 0.351 | SpI | 31 | 9.81523 | - | 1 | 0 | Y | n16-27c31/32o | Unknown | 2 | 50 | |||||||
| HOFP_ECOLI | P45750 | hofP yrfA | yrfA | b3392 JW5694 | periplasmic protein, DNA utilization HofP | No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. | Periplasmic | G | IM (predicted-detected) | Cytoplasmic | no | yes | N | 21 | 0.138 | CYT | 0 | -0.200913 | - | 0 | 0 | Y | n6-16c20/21o | Unknown | 2 | ||||||||||||
| GSPD_ECOLI | P45758 | gspD yheF | yheF | b3325 JW5707 | OM b-barrel protein (SEC through IM, inserted in OM by BAM), Putative type II secretion system D GspD | Outer Membrane b-barrel protein | Potential substrate of periplasmic peptidyl prolyl cis???trans isomerase SurA; sequence contains known binding motif of SurA [RYEF]X[RYEF][P]. Homolog to type two secretion system proteins PulD. Potential outer membrane b-barel protein since it is homolog to pulD major component of type two secretion system. GspD is the outer membrane secretin of type II secretion system (T2SS) which forms a multimeric pore for translocation of secreted proteins. GspD and the inner membrane protein GspC interact with each other via periplasmic domains (observed in the crystal structure). PMID: 21931548 PMID: 11266368 | PMID: 11118204 PMID: 11266368 PMID: 14966662 PMID: 18498364 PMID: 21931548 | Outer Membrane b-barrel protein | H | N/A | Cell outer membrane | Periplasmic (Experimental) | SEC | SURA | BAM | no | yes | Y | 24 | 0.553 | SpI | 23 | 14.273 | - | 0 | 0 | Y | n7-16c23/24o | OuterMembrane | 10 | 34 | |||||
| GSPJ_ECOLI | P45761 | gspJ yheI | yheI | b3331 JW3293 | periplasmic protein (SEC through IM), Putative type II secretion system J GspJ | Periplasmic | Potential component of type II secretion system. GspJ is one of four low abundance pseudopillins together with GspI, H and K. These assemble together with the major pseudopillin GspG and may be involved in initiation, stabilization, regulation or capping PMID: 11266368 | PMID: 11118204 PMID: 11266368 PMID: 14966662 PMID: 18241884 PMID: 21890895 PMID: 23006128 | Periplasmic | G | N/A | Periplasmic (Experimental) | SEC | T2S | no | yes | N | 28 | 0.304 | SpI | 34 | 2.3055 | - | 1 | 1 | N | i7-31o | CytoplasmicMembrane | 9 | ||||||||
| GSPK_ECOLI | P45762 | gspK yheJ | yheJ | b3332 JW3294 | periplasmic protein (SEC through IM), Putative type II secretion system K GspK | Periplasmic | Potential component of type II secretion system. GspK is one of four low abundance pseudopillins together with GspI, J and H. These assemble together with the major pseudopillin GspG and may be involved in initiation, stabilization, regulation or capping PMID: 11266368 | PMID: 11118204 PMID: 11266368 PMID: 14966662 PMID: 18241884 PMID: 21890895 PMID: 23006128 | Periplasmic | G | N/A | Cell inner membrane | Periplasmic (Experimental) | SEC | T2S | no | yes | N | 25 | 0.537 | SpI | 24 | 8.69449 | - | 1 | 0 | Y | n8-19c24/25o | CytoplasmicMembrane | 9 | 30 | ||||||
| YHDW_ECOLI | P45766 | yhdW | b3268 JW3236 | periplasmic protein (SEC through IM), Putative amino-acid ABC transporter-binding YhdW | Periplasmic | Disulfide bond-containing protein PMID: 25664544 | PMID: 25664544 | Periplasmic | G | Soluble IM Fraction | Periplasm | Cytoplasmic | SEC | pseudogene (Uniprot) | no | no | Y | 25 | 0.628 | SpI | 24 | 20.0416 | - | 0 | 0 | Y | n6-17c24/25o | Periplasmic | 10 | 89 | |||||||
| CPOB_ECOLI | P45955 | cpoB ybgF | ybgF | b0742 JW0732 | periplasmic protein (SEC through IM), Cell division coordinator CpoB | Periplasmic | SecB substrate. The N-terminal coiled coil of YbgFvforms a stable trimer and controls the oligomeric status of YbgF.The stable YbgF trimer undergoes a marked change in oligomeric state on binding TolA, forming a one-to-one complex with the Tol protein. PMID: 11703658 | PMID: 11703658 PMID: 16352602 PMID: 20816983 | Periplasmic | G | N/A | Periplasm | Periplasmic (Experimental) | SECB | SEC | yes | yes | Y | 27 | 0.495 | SpI | 26 | 13.974 | - | 0 | 0 | Y | n9-20c26/27o | Periplasmic | 9 | 19 | ||||||
| YAJI_ECOLI | P46122 | yajI | b0412 JW5056 | OM lipoprotein (SEC through IM, inserted in OM by LOL), YajI | Outer Membrane Lipoprotein | I | N/A | Cell membrane | Outer membrane Lipoprotein | SEC | LOL | no | yes | Y | 24 | 0.215 | SpII | 19 | 18.9902 | Pos+2=V | 0 | 0 | Y | n8-18c23/24o | Unknown | 2 | 88 | ||||||||||
| YFIM_ECOLI | P46126 | yfiM | b2586 JW2570 | IM lipoprotein (SEC through IM, inserted in OM by LOL), YfiM | Contains the following signature IPR018736 Protein of unknown function DUF2279,Periplasmic lipoprotein | Inner Membrane Lipoprotein | E | N/A | Cytoplasmic | SEC | LOL | yes | yes | Y | 20 | 0.599 | SpII | 14 | 15.2395 | Pos+2=S | 0 | 0 | Y | n3-13c18/19o | CytoplasmicMembrane | 9 | 12 | ||||||||||
| YBHC_ECOLI | P46130 | ybhC | b0772 JW0755 | OM lipoprotein (SEC through IM), inserted in OM by LOL), Putative acyl-CoA thioester hydrolase YbhC | Outer Membrane Lipoprotein | Proven pignal peptidase II substrate. Outer membrane protein determined by 2D gel electrophoresis (Lopez-Campistrous et al 2005, Molloy et al 2000) PMID: 10806384 Disulfide bridge (185 197) PMID: 19452549 | PMID: 10806384 PMID: 15174130 PMID: 15911532 PMID: 19452549 PMID: 20932056 | Outer Membrane Lipoprotein | I | Soluble IM Fraction | Cell outer membrane | Outer membrane Lipoprotein (Experimental) | SEC | LOL | yes | yes | Y | 24 | 0.274 | SpII | 21 | 24.9976 | Pos+2=S | 0 | 0 | Y | n9-19c24/25o | OuterMembrane | 9 | 71 | |||||||
| YGGM_ECOLI | P46142 | yggM | b2956 JW2923 | periplasmic protein (SEC through IM), YggM | Periplasmic | G | N/A | Periplasmic | SEC | yes | yes | Y | 22 | 0.874 | SpI | 21 | 16.8631 | - | 0 | 0 | Y | n5-16c21/22o | Periplasmic | 9 | 32 | ||||||||||||
| YHDP_ECOLI | P46474 | yhdP yhdQ yhdR | yhdQ yhdR | b4472 JW5542 | OM b-barrel protein (SEC through IM, inserted in OM by BAM), YhdP | Outer Membrane b-barrel protein | H | N/A | Cytoplasmic | SEC | BAM | pseudogene (Ochman & Davalos, 2006) | yes | yes | N | 30 | 0.25 | SpI | 29 | 5.14802 | - | 1 | 0 | Y | n7-22c29/30o | CytoplasmicMembrane | 9 | ||||||||||
| AMO_ECOLI | P46883 | tynA maoA | maoA | b1386 JW1381 | periplasmic protein (SEC through IM), Primary amine oxidase TynA | Periplasmic | Potential Skp substrate PMID: 19953543 PMID: 8631685 | PMID: 19953543 PMID: 8631685 | Periplasmic | G | N/A | Periplasm | Periplasmic (Experimental) | SEC | SKP | no | yes | Y | 31 | 0.714 | SpI | 30 | 12.7601 | - | 0 | 0 | Y | n13-24c30/31o | Periplasmic | 10 | 97 | ||||||
| TORZ_ECOLI | P46923 | torZ bisZ | bisZ | b1872 JW1861 | periplasmic protein (TAT-SEC through IM), Trimethylamine-N-oxide reductase 2 TorZ | Periplasmic | TAT secretion system. PMID: 11004177 | PMID: 11004177 PMID: 17218314 | Periplasmic | G | N/A | Periplasm | Periplasmic (Experimental) | TAT,SEC | yes | yes | N | 32 | 0.688 | SpI | 31 | 17.7989 | - | 0 | 0 | Y | n12-23c31/32o | Periplasmic | 10 | 33 | |||||||
| CSGC_ECOLI | P52107 | csgC | b1043 JW1026 | periplasmic protein (SEC through IM), Curli assembly CsgC | Periplasmic | Uniprot mentions an 8aa signal peptide but Signal P prediction is for a "typical" 22 residue peptide Disulfide bond-containing protein PMID: 25664544 Disulfide bond-containing protein PMID: 25664544 | PMID: 19131513 PMID: 21645131 PMID: 25664544 | Periplasmic | G | N/A | Periplasm | Periplasmic | SEC | no | yes | N | 22 | 0.179 | SpI | 22 | 2.07115 | - | 0 | 0 | Y | n4-16c21/22o | Unknown | 2 | 68 | ||||||||
| YFJT_ECOLI | P52135 | yfjT | b2637 JW2618 | periplasmic protein (SEC through IM), YfjT | Periplasmic | G | N/A | Periplasmic | SEC | Prophage Integrase (EcoGene) | no | no | Y | 24 | 0.673 | SpI | 23 | 19.2157 | - | 0 | 0 | Y | n9-19c23/24o | Unknown | 2 | 22 | |||||||||||
| YPJA_ECOLI | P52143 | ypjA | b2647 JW5422 | OM b-barrel protein (SEC through IM, T5S secreted through OM), YpjA | Outer Membrane b-barrel protein | Ag43 homologue; predicted autotransporter. Contains autotransporter beta-domain <a href="http://www.ebi.ac.uk/interpro/entry/IPR005546/proteins-matched?species=83333">(IPR005546)</a>. Potential Srp-dependent autotransporter. Homologous to hemoglobin-binding protease hbp. Hbp is the only known autotransporter that is secreted through the integral membrane in a Srp-dependent manner. | PMID: 12466262 PMID: 15590781 PMID: 22905239 | Outer Membrane b-barrel protein | H | N/A | Cell outer membrane | Outer Membrane ??-barrel protein | SRP | SEC | Autotransporter(T5S) | no | yes | Y | 30 | 0.688 | SpI | 29 | 11.7048 | - | 1 | 0 | Y | n13-24c29/30o | Unknown | 5 | |||||||
| EMRK_ECOLI | P52599 | emrK | b2368 JW2365 | periplasmic protein (SEC through IM), Probable multidrug resistance EmrK | Periplasmic | PMID: 11914367 | Periplasmic | G | N/A | Cell inner membrane | Periplasmic (Experimental) | SEC | yes | yes | N | 34 | 0.579 | SpI | 33 | 15.7518 | - | 1 | 0 | Y | n17-28c33/34o | CytoplasmicMembrane | 9 | 38 | |||||||||
| FLIK_ECOLI | P52614 | fliK flaE flaR | flaE flaR | b1943 JW1927 | Flagellar cytoplasmic & extra-cellular protein (Flagellum secreted), hook-length control protein FliK | Cytoplasmic, Extra-cellular | Hook-length control protein. Protein FliK detects the point where the flagellar hook substructure has reached its optimal length. Then it terminates hook export and assembly and transmits a signal to begin filament export. | PMID: 17456739 | Cytoplasmic, Extra-cellular | A, X | N/A | Cytoplasmic | flagellum | Flagellum | Fla | no | yes | N | 47 | 0.186 | CYT | 0 | -0.200913 | - | 0 | 0 | N | i | Extracellular | 10 | 97 | ||||||
| HSLJ_ECOLI | P52644 | hslJ ydbI | ydbI | b1379 JW1374 | OM lipoprotein (SEC through IM, inserted in OM by LOL), HslJ | Outer Membrane Lipoprotein | Proven pignal peptidase II substrate. PMID: 15174130 | PMID: 15174130 | Outer Membrane Lipoprotein | I | N/A | Outer membrane Lipoprotein (Experimental) | SEC | LOL | yes | yes | Y | 25 | 0.238 | SpII | 16 | 16.4623 | Pos+2=V | 0 | 0 | Y | n4-15c20/21o | Unknown | 2 | 106 | |||||||
| RZOD_ECOLI | P58041 | rzoD | b4510 JW5080 | OM lipoprotein (SEC through IM), inserted in OM by LOL), Prophage RzoD (o-spanin) | Outer Membrane Lipoprotein | Disulfide bridge (29 29) | Outer Membrane Lipoprotein | I | N/A | Cell outer membrane | Outer membrane Lipoprotein | SEC | LOL | Prophage Integrase (EcoGene) | no | no | Y | 20 | 0.187 | SpII | 19 | 19.9508 | Pos+2=T | 0 | 0 | Y | n8-18c22/23o | Unknown | 2 | 147 | |||||||
| RZOR_ECOLI | P58042 | rzoR | b4528 JW5213 | OM lipoprotein (SEC through IM), inserted in OM by LOL), Prophage RzoR (o-spanin) | Outer Membrane Lipoprotein | Disulfide bridge (29 29) | Outer Membrane Lipoprotein | I | N/A | Cell outer membrane | Outer membrane Lipoprotein | SEC | LOL | Prophage Integrase (EcoGene) | no | no | Y | 20 | 0.189 | SpII | 19 | 20.8844 | Pos+2=T | 0 | 0 | Y | n8-18c22/23o | Unknown | 2 | 88 | |||||||
| LOLA_ECOLI | P61316 | lolA lplA yzzV | lplA yzzV | b0891 JW0874 | periplasmic protein (SEC through IM), Outer-carrier LolA | Periplasmic | PMID: 15276320 PMID: 7628437 PMID: 9298646 | Periplasmic | G | N/A | Periplasm | Periplasmic (Experimental) | SEC | yes | yes | Y | 22 | 0.602 | SpI | 21 | 20.5659 | - | 0 | 0 | Y | n4-15c21/22o | Periplasmic | 10 | 70 | ||||||||
| LOLB_ECOLI | P61320 | lolB hemM ychC | hemM ychC | b1209 JW1200 | OM lipoprotein (SEC through IM), inserted in OM by LOL), Outer-LolB | Outer Membrane Lipoprotein | Proven pignal peptidase II substrate. Outer membrane protein determined by LC-MS (Gevaert et al 2002) Outer membrane protein determined by 2D gel electrophoresis (Lopez-Campistrous et al 2005, Fountoulakis et al 2003) Identified in nucleoids collected by sucrose gradient centrifugation and analyzed by liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS). PMID: 21541338 PMID: 16091355 | PMID: 12488465 PMID: 12624733 PMID: 15174130 PMID: 15276320 PMID: 15911532 PMID: 16091355 PMID: 20932056 PMID: 21541338 | Outer Membrane Lipoprotein | I | Soluble IM Fraction | Cell outer membrane; Lipid-anchor | Outer membrane Lipoprotein (Experimental) | SEC | LOL | yes | yes | Y | 24 | 0.169 | SpII | 21 | 13.4863 | Pos+2=S | 0 | 0 | Y | n11-22c27/28o | Periplasmic | 9 | 85 | ||||||
| SECM_ECOLI | P62395 | secM srrA yacA | srrA yacA | b0097 JW5007 | cytoplasmic & periplasmic protein (SEC through IM), Secretion monitor SecM | Cytoplasmic, Periplasmic | SecM regulates secA expression by translational coupling of the secM secA operon. Fusion of SecM with alkaline phosphatase activity exhibits substantial activity in the periplasm. SecM periplasmic form is rapidly degraded. Potential substrate of periplasmic peptidyl prolyl cis???trans isomerase SurA; sequence contains known binding motif of SurA [RYEF]X[RYEF][P]. PMID: 11597451 | PMID: 11597451 PMID: 1834634 PMID: 18498364 | Cytoplasmic, Periplasmic | A, G | N/A | Cytoplasm; cytosol | Periplasmic (Experimental) | SEC | SURA | yes | yes | Y | 38 | 0.527 | SpI | 37 | 11.5151 | - | 1 | 0 | Y | n20-31c37/38o | Cytoplasmic | 10 | |||||||
| AMIC_ECOLI | P63883 | amiC ygdN | ygdN | b2817 JW5449 | periplasmic protein (TAT-SEC through IM), N-acetylmuramoyl-L-alanine amidase AmiC | Periplasmic | TAT secretion system. PMID: 12787347 | PMID: 12787347 PMID: 17218314 | Periplasmic | G | N/A | Periplasm | Periplasmic (Experimental) | TAT,SEC | yes | yes | N | 32 | 0.61 | SpI | 31 | 9.65072 | - | 0 | 0 | Y | n13-23c31/32o | Unknown | 2 | 33 | |||||||
| YDDW_ECOLI | P64426 | yddW | b1491 JW1486 | OM lipoprotein (SEC through IM), inserted in OM by LOL), UPF0748 YddW | Outer Membrane Lipoprotein | Proven pignal peptidase II substrate. PMID: 15174130 | PMID: 15174130 | Outer Membrane Lipoprotein | I | N/A | Cell membrane | Outer membrane Lipoprotein (Experimental) | SEC | LOL | yes | yes | Y | 36 | 0.168 | SpII | 27 | 12.5186 | Pos+2=K | 0 | 0 | Y | n16-26c30/31o | Unknown | 2 | 62 | |||||||
| YNBE_ECOLI | P64448 | ynbE | b1382 JW1377 | OM lipoprotein (SEC through IM, inserted in OM by LOL), YnbE | Outer Membrane Lipoprotein | Proven pignal peptidase II substrate. PMID: 15174130 | PMID: 15174130 | Outer Membrane Lipoprotein | I | N/A | Outer membrane Lipoprotein (Experimental) | SEC | LOL | no | yes | N | 25 | 0.214 | SpII | 16 | 4.04646 | Pos+2=T | 0 | 0 | Y | n4-15c24/25o | Unknown | 2 | |||||||||
| YDCL_ECOLI | P64451 | ydcL | b1431 JW1427 | OM lipoprotein (SEC through IM, inserted in OM by LOL), YdcL | Outer Membrane Lipoprotein | Proven pignal peptidase II substrate. PMID: 15174130 | PMID: 15174130 PMID: 20932056 | Outer Membrane Lipoprotein | I | N/A | Cell membrane | Outer membrane Lipoprotein (Experimental) | SEC | LOL | no | yes | Y | 25 | 0.223 | SpII | 20 | 19.8371 | Pos+2=A | 0 | 0 | Y | n9-18c23/24o | Unknown | 2 | ||||||||
| YNCJ_ECOLI | P64459 | yncJ | b1436 JW1432 | periplasmic protein (SEC through IM), YncJ | Periplasmic | G | N/A | Periplasmic | SEC | no | yes | Y | 23 | 0.752 | SpI | 22 | 8.63896 | - | 0 | 0 | Y | n5-16c22/23o | Unknown | 2 | 38 | ||||||||||||
| YOAF_ECOLI | P64493 | yoaF | b1793 JW1782 | OM lipoprotein (SEC through IM, inserted in OM by LOL), YoaF | Outer Membrane Lipoprotein | Proven pignal peptidase II substrate. PMID: 15174130 | PMID: 15174130 | Outer Membrane Lipoprotein | I | N/A | Outer membrane Lipoprotein (Experimental) | SEC | LOL | no | yes | Y | 24 | 0.245 | SpII | 16 | 20.7594 | Pos+2=S | 0 | 0 | Y | n4-15c23/24o | Unknown | 2 | 90 | ||||||||
| YEBY_ECOLI | P64506 | yebY | b1839 JW1828 | periplasmic protein (SEC through IM), YebY | Periplasmic | Identified DsbA substrate PMID: 19953543 | PMID: 19953543 | Periplasmic | G | N/A | Periplasmic | SEC | DSBA | yes | yes | Y | 21 | 0.862 | SpI | 20 | 19.6801 | - | 1 | 0 | Y | n5-12c20/21o | Unknown | 2 | 35 | ||||||||
| RCNB_ECOLI | P64534 | rcnB yohN | yohN | b2107 JW5346 | periplasmic protein (SEC through IM), Nickel/cobalt homeostasis RcnB | Periplasmic | PMID: 21665978 | Periplasmic | G | N/A | Periplasm | Periplasmic | SEC | no | yes | Y | 22 | 0.81 | SpI | 21 | 23.7077 | - | 1 | 0 | Y | n8-16c21/22o | Unknown | 2 | 79 | ||||||||
| YPEC_ECOLI | P64542 | ypeC | b2390 JW2387 | periplasmic protein (SEC through IM), YpeC | Periplasmic | G | N/A | Periplasmic | SEC | no | yes | Y | 22 | 0.729 | SpI | 21 | 14.9819 | - | 0 | 0 | Y | n4-15c21/22o | Unknown | 2 | 14 | ||||||||||||
| YFIR_ECOLI | P64548 | yfiR | b2603 JW2584 | periplasmic protein (SEC through IM), YfiR | Periplasmic | Disulfide bond-containing protein PMID: 25664544 | PMID: 25664544 | Periplasmic | G | N/A | Periplasm | Periplasmic | SEC | yes | yes | Y | 23 | 0.684 | SpI | 22 | 13.4131 | - | 0 | 0 | Y | n7-17c22/23o | Unknown | 6 | 21 | ||||||||
| YRAP_ECOLI | P64596 | yraP | b3150 JW3119 | OM lipoprotein (SEC through IM, inserted in OM by LOL), YraP | Outer Membrane Lipoprotein | Contains 2 BON domains <a target="_blank" href="http://www.ebi.ac.uk/interpro/entry/IPR007055">IPR007055</a>. BON domains are putative membrane-binding domains PMID: 12878000.The BON domain is typically ~60 residues long and has an alpha/beta predicted fold. The domain architecture of two BON domains alone suggests that these domains contact the surfaces of phospholipids, with each domain contacting a membrane. These are found in a family of osmotic shock protection proteins (e.g. P27291). It is also found in some Secretins and a group of potential haemolysins. Its likely function is attachment to phospholipid membranes. Is homologous to the OsmY_Ecoli protein. There is a conserved glycine residue and several hydrophobic regions. This pattern of conservation is more suggestive of a binding or structural function rather than a catalytic function. Most proteobacteria seem to possess one or two BON-containing proteins, typically of the OsmY-type proteins. Outside of this group the distribution is more disparate. OsmY protein is a 20 kDa outer membrane or periplasmic protein that is expressed in response to a variety of stress conditions. In particular, helping to provide protection against osmotic shock. One hypothesis is that OsmYPrevents shrinkage of the cytoplasmic compartment by contacting the phospholipid interfaces surrounding the periplasmic space. Proven pignal peptidase II substrate PMID: 15174130. Detected in the outer membrane fraction, with mass spectrometric analysis coupled by SDS gel electrophoresisPMID: 20932056. Outer membrane protein determined by LC-MS PMID: 12488465. DacB, DcrB, HybA, and YraP were assigned to either the inner or outer membrane. These apparent differences could be reasonably accommodated if these proteins were peripherally associated with the side of either the inner or outer membrane that faces the periplasmic space PMID: 15911532. PMID: 15174130 | PMID: 12488465 PMID: 12878000 PMID: 15174130 PMID: 15911532 PMID: 15968066 PMID: 17904518 PMID: 20932056 | Outer Membrane Lipoprotein | I | N/A | Periplasm | Outer membrane Lipoprotein(experimental) | SEC | LOL | yes | yes | Y | 23 | 0.327 | SpII | 18 | 13.0401 | Pos+2=V | 1 | 0 | Y | n3-16c21/22o | Periplasmic | 9 | 95 | |||||||
| MLAD_ECOLI | P64604 | mlaD yrbD | yrbD | b3193 JW3160 | periplasmic protein (SEC through IM), Interphospholipid transport system binding MlaD | Periplasmic | G | Soluble IM Fraction | Cell inner membrane | Periplasmic | SEC | yes | yes | N | 29 | 0.187 | SpI | 28 | 7.17952 | - | 1 | 0 | Y | n8-23c28/29o | Unknown | 2 | 60 | ||||||||||
| YHCN_ECOLI | P64614 | yhcN | b3238 JW5540 | periplasmic protein (SEC through IM), YhcN | Periplasmic | G | N/A | Periplasm | Periplasmic | SEC | yes | yes | Y | 23 | 0.834 | SpI | 22 | 22.7033 | - | 0 | 0 | Y | n7-18c22/23o | Unknown | 2 | ||||||||||||
| YHDV_ECOLI | P64622 | yhdV | b3267 JW3235 | OM lipoprotein (SEC through IM, inserted in OM by LOL), YhdV | Outer Membrane Lipoprotein | Proven signal peptidase II substrate. PMID: 15174130 | PMID: 15174130 | Outer Membrane Lipoprotein | I | IM (predicted) | Cell membrane | Outer membrane Lipoprotein (Experimental) | SEC | LOL | no | yes | Y | 21 | 0.268 | SpII | 16 | 14.2179 | Pos+2=A | 1 | 1 | Y | n4-15c20/21o50-71i | Unknown | 2 | 30 | |||||||
| YHFL_ECOLI | P64627 | yhfL | b3369 JW3332 | OM lipoprotein (SEC through IM, inserted in OM by LOL), YhfL | Outer Membrane Lipoprotein | Proven pignal peptidase II substrate. PMID: 15174130 | PMID: 15174130 PMID: 21890895 | Outer Membrane Lipoprotein | I | N/A | Membrane | Outer membrane Lipoprotein (Experimental) | SEC | LOL | no | yes | N | 22 | 0.162 | SpII | 19 | 15.9797 | Pos+2=T | 0 | 0 | Y | n7-17c22/23o | Unknown | 2 | ||||||||
| YFGH_ECOLI | P65290 | yfgH | b2505 JW5400 | OM lipoprotein (SEC through IM, inserted in OM by LOL), YfgH | Outer Membrane Lipoprotein | Proven pignal peptidase II substrate. PMID: 15174130 | PMID: 15174130 | Outer Membrane Lipoprotein | I | Soluble IM Fraction | Cell membrane | Outer membrane Lipoprotein (Experimental) | SEC | LOL | yes | yes | Y | 27 | 0.349 | SpII | 21 | 24.716 | Pos+2=Q | 0 | 0 | Y | n7-17c22/23o | Unknown | 2 | 73 | |||||||
| YGDI_ECOLI | P65292 | ygdI | b2809 JW5448 | OM lipoprotein (SEC through IM, inserted in OM by LOL), YgdI | Outer Membrane Lipoprotein | Outer membrane protein determined by LC-MS (Gevaert et al 2002) Identified in nucleoids collected by sucrose gradient centrifugation and analyzed by liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS). PMID: 21541338 PMID: 15174130 | PMID: 12488465 PMID: 15174130 PMID: 21541338 | Outer Membrane Lipoprotein | I | N/A | Cell membrane | Outer membrane Lipoprotein (Experimental) | SEC | LOL | no | yes | Y | 22 | 0.339 | SpII | 19 | 21.6734 | Pos+2=S | 1 | 0 | Y | n6-17c22/23o | Unknown | 2 | 119 | |||||||
| YGDR_ECOLI | P65294 | ygdR | b2833 JW2801 | OM lipoprotein (SEC through IM, inserted in OM by LOL), YgdR | Outer Membrane Lipoprotein | Proven pignal peptidase II substrate. PMID: 15174130 | PMID: 15174130 | Outer Membrane Lipoprotein | I | N/A | Cell membrane | Outer membrane Lipoprotein (Experimental) | SEC | LOL | no | yes | N | 28 | 0.252 | SpII | 19 | 15.677 | Pos+2=S | 1 | 0 | Y | n6-17c22/23o | Unknown | 2 | ||||||||
| YQHH_ECOLI | P65298 | yqhH | b3014 JW2982 | OM lipoprotein (SEC through IM, inserted in OM by LOL), YqhH | Outer Membrane Lipoprotein | Proven pignal peptidase II substrate. PMID: 15174130 | PMID: 15174130 | Outer Membrane Lipoprotein | I | N/A | Cell outer membrane | Outer membrane Lipoprotein (Experimental) | SEC | LOL | yes | yes | N | 24 | 0.242 | SpII | 19 | 15.1531 | Pos+2=V | 1 | 0 | Y | n4-17c22/23o | OuterMembrane | 9 | 17 | |||||||
| BEPA_ECOLI | P66948 | bepA yfgC | yfgC | b2494 JW2479 | periplasmic protein (SEC through IM), Beta-barrel assembly-enhancing protease BepA | Periplasmic | BepA maintains the integrity of OM both by promoting assembly of OMPs and by proteolytically eliminating OMPs when their correct assembly was compromised. BepA is a metalloprotease that cleaves its targets preferentially between Phe-Phe residues. Potential Skp substrate PMID: 19953543 | PMID: 19953543 PMID: 22491786 PMID: 22901060 PMID: 24003122 | Periplasmic | G | N/A | Periplasm | Periplasmic | SEC | SKP | yes | yes | Y | 28 | 0.636 | SpI | 27 | 15.2429 | - | 0 | 0 | Y | n9-19c27/28o | Unknown | 2 | 10 | ||||||
| YNFC_ECOLI | P67553 | ynfC | b1585 JW5258 | IM lipoprotein (SEC through IM), inserted in OM by LOL), UPF0257 YnfC | Inner Membrane Lipoprotein | Proven pignal peptidase II substrate. | PMID: 15174130 | Inner Membrane Lipoprotein | E | N/A | Cell membrane | Inner membrane lipoprotein | SEC | LOL | yes | yes | N | 21 | 0.178 | SpII | 16 | 14.4125 | Pos+2=D | 0 | 0 | Y | n5-14c19/20o | Cytoplasmic | 8 | ||||||||
| RCSF_ECOLI | P69411 | rcsF | b0196 JW0192 | OM lipoprotein (SEC through IM, inserted in OM by LOL), RcsF | Outer Membrane Lipoprotein | Proven pignal peptidase II substrate. Identified DsbA substrate PMID: 19953543 PMID: 15174130 Disulfide bridge (74 118;109 124) Disulfide bond-containing protein PMID: 25664544 | PMID: 15174130 PMID: 16740933 PMID: 19953543 PMID: 20932056 PMID: 23813676 PMID: 25664544 | Outer Membrane Lipoprotein | I | Soluble IM Fraction | Cell outer membrane | Outer membrane Lipoprotein (Experimental) | SEC | DSBA | LOL | yes | yes | Y | 23 | 0.182 | SpII | 15 | 13.2891 | Pos+2=S | 0 | 0 | Y | n3-13c21/22o | OuterMembrane | 10 | 81 | ||||||
| PGAA_ECOLI | P69434 | pgaA ycdS | ycdS | b1024 JW1010 | OM b-barrel protein (SEC through IM, inserted in OM by BAM), Poly-beta-1,6-N-acetyl-D-glucosamine export PgaA | Outer Membrane b-barrel protein | H | N/A | Cell outer membrane | Outer Membrane ??-barrel protein | SEC | BAM | no | yes | Y | 33 | 0.331 | SpI | 32 | 12.415 | - | 0 | 0 | Y | n18-26c32/33o | Unknown | 2 | 6 | |||||||||
| MBHT_ECOLI | P69741 | hybO yghV | yghV | b2997 JW2965 | periplasmic peripheral IM protein (TAT-SEC through IM), Hydrogenase-2 small chain HybO | Peripheral inner membrane protein facing the periplasm | Copurifies with trigger factor in pull down assays. Exported by the Tat system. Experimentally proven signal peptide cleavage site. Under anaerobic conditions Escherichia coli synthesizes three membrane-associated [NiFe]-hydrogenases (Hyd). Hyd-1 and Hyd-2 are respiratory hydrogenases with their active sites facing the periplasm. PMID: 22769583 PMID: 9738917 | PMID: 17218314 PMID: 19737520 PMID: 22769583 PMID: 9738917 | Peripheral inner membrane protein facing the periplasm | F2 | IM (predicted) | Cell membrane; Peripheral membrane protein; Periplasmic side Periplasm | Membrane anchored (Experimental) | TF | TAT,SEC | yes | yes | N | 38 | 0.248 | SpI | 37 | 7.00921 | - | 1 | 1 | Y | n21-32c37/38o332-355i | CytoplasmicMembrane | 10 | |||||||
| LPP_ECOLI | P69776 | lpp mlpA mulI | mlpA mulI | b1677 JW1667 | OM lipoprotein & extra-cellular peripheral OM protein (SEC through IM, inserted in OM by LOL), Lpp | Outer Membrane Lipoprotein, Peripheral outer membrane protein facing the extra-cellular space | Trigger factor-sensitive aggregation. Proven pignal peptidase II substrate. Outer membrane protein determined by 2D gel electrophoresis (Lopez-Campistrous et al 2005). Covalently attached to the peptidoglycan cell wall by the ??-amino group of its C-terminal Lys residue; stabilizes the bacterial envelope. Identified in nucleoids collected by sucrose gradient centrifugation and analyzed by liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS). PMID: 21541338 PMID: 4575979 | PMID: 15174130 PMID: 15911532 PMID: 16079137 PMID: 19737520 PMID: 21219470 PMID: 21541338 PMID: 3013869 PMID: 4575979 | Outer Membrane Lipoprotein, Peripheral outer membrane protein facing the extra-cellular space | I, F4 | Soluble IM Fraction | Cell outer membrane | Outer membrane Lipoprotein (Experimental) | TF | SEC | LOL | yes | yes | Y | 26 | 0.325 | SpII | 20 | 26.7873 | Pos+2=S | 0 | 0 | Y | n6-17c25/26o | OuterMembrane | 10 | 58 | |||||
| NANC_ECOLI | P69856 | nanC yjhA | yjhA | b4311 JW5778 | periplasmic protein (SEC through IM), Probable N-acetylneuraminic acid channel NanC | Periplasmic | G | N/A | Cell outer membrane | Periplasmic | SEC | no | yes | Y | 24 | 0.708 | SpI | 23 | 19.1942 | - | 0 | 0 | Y | n6-15c23/24o | OuterMembrane | 10 | 8 | ||||||||||
| WCAM_ECOLI | P71244 | wcaM yefK | yefK | b2043 JW2028 | periplasmic protein (TAT through IM), Colanic acid biosynthesis WcaM | Periplasmic | TAT secretion system. | PMID: 17218314 | Periplasmic | G | N/A | Periplasmic | TAT | no | yes | Y | 29 | 0.421 | SpI | 28 | 15.7954 | - | 0 | 0 | Y | n10-21c28/29o | Unknown | 2 | |||||||||
| YAAX_ECOLI | P75616 | yaaX | b0005 JW0004 | periplasmic protein (SEC through IM), YaaX | Periplasmic | G | N/A | Periplasmic | SEC | no | yes | Y | 24 | 0.841 | SpI | 23 | 19.693 | - | 1 | 0 | Y | n6-17c23/24o | Unknown | 2 | 77 | ||||||||||||
| RCLB_ECOLI | P75687 | rclB ykgI | ykgI | b0303 JW5039 | periplasmic protein (SEC through IM), RclB | Periplasmic | G | N/A | Periplasm | Periplasmic | SEC | no | yes | Y | 23 | 0.662 | SpI | 22 | 16.2846 | - | 0 | 0 | Y | n6-17c22/23o | Unknown | 2 | |||||||||||
| YAHO_ECOLI | P75694 | yahO | b0329 JW0321 | periplasmic protein (SEC through IM), YahO | Periplasmic | G | N/A | Periplasm | Periplasmic | SEC | no | yes | Y | 22 | 0.85 | SpI | 21 | 13.1005 | - | 0 | 0 | Y | n8-16c21/22o | Unknown | 2 | 85 | |||||||||||
| SFMH_ECOLI | P75715 | sfmH | b0533 JW5071 | Fimbrial periplasmic protein (SEC through IM), SfmH | Periplasmic | Potential fimbrial sfm operon (class ??1); Potential tip-adhesin. Potential substrate of periplasmic peptidyl prolyl cis???trans isomerase SurA; sequence contains known binding motif of SurA [RYEF]X[RYEF][P]. | PMID: 18498364 PMID: 19820722 PMID: 20345943 | Periplasmic | G | N/A | Fimbrium | Cytoplasmic | fimbriae | SEC | SURA | CU | no | yes | Y | 19 | 0.186 | SpI | 24 | 6.26896 | - | 0 | 0 | Y | n2-9c14/15o | Unknown | 2 | 17 | |||||
| CHIP_ECOLI | P75733 | chiP ybfM | ybfM | b0681 JW0667 | OM b-barrel protein (SEC through IM, inserted in OM by BAM), Chitoporin ChiP | Outer Membrane b-barrel protein | H | N/A | Cell outer membrane | Outer Membrane ??-barrel protein | SEC | BAM | no | yes | Y | 33 | 0.272 | SpI | 32 | 12.9387 | - | 0 | 0 | Y | n10-20c32/33o | Unknown | 2 | 1 | |||||||||
| CHIQ_ECOLI | P75734 | chiQ ybfN | ybfN | b0682 JW0668 | OM lipoprotein (SEC through IM, inserted in OM by LOL), ChiQ | Outer Membrane Lipoprotein | Proven pignal peptidase II substrate. PMID: 15174130 | PMID: 15174130 | Outer Membrane Lipoprotein | I | N/A | Cell membrane | Outer membrane Lipoprotein (Experimental) | SEC | LOL | yes | yes | Y | 19 | 0.487 | SpII | 16 | 19.5262 | Pos+2=A | 0 | 0 | Y | n4-14c18/19o | Unknown | 2 | 77 | ||||||
| YBFP_ECOLI | P75737 | ybfP | b0689 JW0676 | OM lipoprotein (SEC through IM, inserted in OM by LOL), YbfP | Outer Membrane Lipoprotein | Proven pignal peptidase II substrate. PMID: 15174130 | PMID: 15174130 | Outer Membrane Lipoprotein | I | N/A | Cell membrane | Outer membrane Lipoprotein (Experimental) | SEC | LOL | no | yes | Y | 25 | 0.444 | SpII | 22 | 15.3591 | Pos+2=A | 1 | 0 | Y | n7-20c24/25o | Unknown | 2 | 51 | |||||||
| YBGO_ECOLI | P75748 | ybgO | b0716 JW5098 | Fimbrial periplasmic protein (SEC through IM), YbgO | Periplasmic | Putative chaperone???usher fimbrial operon yeh (class ??); Unkown function. Disulfide bond-containing protein PMID: 25664544 | PMID: 20345943 PMID: 25664544 | Periplasmic | G | N/A | Periplasmic | fimbriae | SEC | no | yes | N | 24 | 0.359 | SpI | 23 | 7.76951 | - | 0 | 0 | Y | n7-18c23/24o | Cytoplasmic | 8 | 53 | ||||||||
| YBGP_ECOLI | P75749 | ybgP | b0717 JW0707 | Fimbrial periplasmic protein (SEC through IM), Chaperone YbgP | Periplasmic | Putative chaperone???usher fimbrial operon yeh (class ??); Chaperone. Potential substrate of periplasmic peptidyl prolyl cis???trans isomerase SurA; sequence contains known binding motif of SurA [RYEF]X[RYEF][P]. | PMID: 18498364 PMID: 20345943 | Periplasmic | G | N/A | Periplasm | Periplasmic | fimbriae | SEC | SURA | no | yes | Y | 21 | 0.795 | SpI | 20 | 5.41341 | - | 0 | 0 | Y | n6-16c20/21o | Periplasmic | 10 | 19 | ||||||
| YBGQ_ECOLI | P75750 | ybgQ | b0718 JW5099 | Fimbrial OM b-barrel protein (SEC through IM, inserted in OM by BAM), YbgQ | Outer Membrane b-barrel protein | Putative chaperone???usher fimbrial operon yeh (class ??); Usher. Disulfide bridge (795 814) | PMID: 20345943 | Outer Membrane b-barrel protein | H | N/A | Cell outer membrane | Outer Membrane ??-barrel protein | fimbriae | SEC | BAM | no | yes | Y | 22 | 0.84 | SpI | 21 | 8.29107 | - | 0 | 0 | Y | n6-16c21/22o | OuterMembrane | 10 | 10 | ||||||
| FIU_ECOLI | P75780 | fiu ybiL | ybiL | b0805 JW0790 | OM b-barrel protein (SEC through IM, inserted in OM by BAM), Catecholate siderophore receptor Fiu | Outer Membrane b-barrel protein | Outer membrane protein determined by 2D gel electrophoresis (Lopez-Campistrous et al 2005, Fountoulakis et al 2003)','Outer membrane protein determined by 2D gel electrophoresis (Lopez-Campistrous et al 2005, Fountoulakis et al 2003, Molloy et al 2000) Potential Skp substrate PMID: 19953543 PMID: 10806384 Disulfide bond-containing protein PMID: 25664544 | PMID: 10806384 PMID: 19953543 PMID: 20932056 PMID: 25664544 | Outer Membrane b-barrel protein | H | N/A | Cell outer membrane | Outer Membrane ??-barrel protein (Experimental) | SEC | SKP | BAM | yes | yes | Y | 34 | 0.525 | SpI | 33 | 11.8291 | - | 1 | 0 | Y | n14-25c33/34o | OuterMembrane | 10 | 10 | |||||
| GSIB_ECOLI | P75797 | gsiB yliB | yliB | b0830 JW5111 | periplasmic protein (SEC through IM), Glutathione-binding GsiB | Periplasmic | Potential substrate of periplasmic peptidyl prolyl cis???trans isomerase SurA; sequence contains known binding motif of SurA [RYEF]X[RYEF][P]. PMID: 15911532 | PMID: 15911532 PMID: 18498364 | Periplasmic | G | N/A | Periplasm | Periplasmic(experimental) | SEC | SURA | yes | yes | Y | 27 | 0.582 | SpI | 26 | 15.0769 | - | 0 | 0 | Y | n10-21c26/27o | Periplasmic | 10 | 40 | ||||||
| YLII_ECOLI | P75804 | yliI | b0837 JW0821 | periplasmic protein (SEC through IM), Aldose sugar dehydrogenase YliI | Periplasmic | Potential substrate of periplasmic peptidyl prolyl cis???trans isomerase SurA; sequence contains known binding motif of SurA [RYEF]X[RYEF][P]. | PMID: 18498364 | Periplasmic | G | N/A | Cell outer membrane | Periplasmic | SEC | SURA | no | yes | Y | 21 | 0.629 | SpI | 20 | 9.24571 | - | 0 | 0 | Y | n5-15c20/21o | CytoplasmicMembrane | 9 | 21 | |||||||
| YBJP_ECOLI | P75818 | ybjP | b0865 JW0849 | OM lipoprotein (SEC through IM, inserted in OM by LOL), YbjP | Outer Membrane Lipoprotein | Proven pignal peptidase II substrate. Outer membrane protein determined by 2D gel electrophoresis (Lopez-Campistrous et al 2005) Identified in nucleoids collected by sucrose gradient centrifugation and analyzed by liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS). PMID: 21541338 Identified DsbA substrate PMID: 19953543 PMID: 10806384 Disulfide bond-containing protein PMID: 25664544 | PMID: 10806384 PMID: 15174130 PMID: 15911532 PMID: 19953543 PMID: 20932056 PMID: 21541338 PMID: 25664544 | Outer Membrane Lipoprotein | I | Soluble IM Fraction | Cell membrane | Outer membrane Lipoprotein (Experimental) | SEC | DSBA | LOL | no | yes | Y | 28 | 0.445 | SpII | 18 | 10.6734 | Pos+2=T | 0 | 0 | Y | n9-19c24/25o | Unknown | 2 | 69 | ||||||
| AMID_ECOLI | P75820 | amiD ybjR | ybjR | b0867 JW0851 | OM lipoprotein (SEC through IM), inserted in OM by LOL), N-acetylmuramoyl-L-alanine amidase AmiD | Outer Membrane Lipoprotein | Proven pignal peptidase II substrate. | PMID: 15174130 | Outer Membrane Lipoprotein | I | N/A | Cell outer membrane | Outer membrane Lipoprotein | SEC | LOL | yes | yes | Y | 21 | 0.182 | SpII | 16 | 18.0923 | Pos+2=A | 0 | 0 | Y | n4-14c19/20o | Unknown | 6 | 18 | ||||||
| SSUA_ECOLI | P75853 | ssuA ycbO | ycbO | b0936 JW0919 | periplasmic protein (SEC through IM), Putative aliphatic sulfonates-binding protein SsuA | Periplasmic | PMID: 10781534 | Periplasmic | G | N/A | Periplasm | Periplasmic (Experimental) | SEC | no | yes | Y | 22 | 0.876 | SpI | 21 | 14.9607 | - | 0 | 0 | Y | n7-18c22/23o | Unknown | 2 | 76 | ||||||||
| ELFA_ECOLI | P75855 | elfA ycbQ | ycbQ | b0938 JW5122 | Fimbrial periplasmic protein (SEC through IM), subunit ElfA | Periplasmic | Potential fimbrial ycb operon (class ??1); Fimbrial Subunit. | PMID: 19820722 PMID: 20345943 | Periplasmic | G | N/A | Fimbrium | Periplasmic | fimbriae | SEC | CU | no | yes | Y | 22 | 0.807 | SpI | 21 | 23.0184 | - | 0 | 0 | Y | n5-16c21/22o | Extracellular | 10 | 85 | |||||
| ELFD_ECOLI | P75856 | elfD ycbR | ycbR | b0939 JW0922 | Fimbrial periplasmic protein (SEC through IM), Probable chaperone ElfD | Periplasmic | Potential fimbrial ycb operon (class ??1); Chaperone. Potential substrate of periplasmic peptidyl prolyl cis???trans isomerase SurA; sequence contains known binding motif of SurA [RYEF]X[RYEF][P]. | PMID: 18498364 | Periplasmic | G | N/A | Periplasm | Periplasmic | fimbriae | SEC | SURA | yes | yes | Y | 27 | 0.835 | SpI | 26 | 18.1825 | - | 0 | 0 | Y | n10-21c26/27o | Unknown | 6 | 29 | |||||
| ELFC_ECOLI | P75857 | elfC ycbS | ycbS | b0940 JW0923 | Fimbrial OM b-barrel protein (SEC through IM, inserted in OM by BAM), Probable usher ElfC | Outer Membrane b-barrel protein | Potential fimbrial ycb operon (class ??1); Usher. | PMID: 20345943 | Outer Membrane b-barrel protein | H | N/A | Cell outer membrane | Outer Membrane ??-barrel protein | fimbriae | SEC | BAM | yes | yes | N | 36 | 0.312 | SpI | 35 | 6.8393 | - | 1 | 0 | Y | n19-30c35/36o | OuterMembrane | 10 | 7 | |||||
| ELFG_ECOLI | P75858 | elfG ycbT | ycbT | b0941 JW0924 | Fimbrial periplasmic protein (SEC through IM), ElfG | Periplasmic | Potential fimbrial ycb operon (class ??1); Potential tip-adhesin. | PMID: 19820722 PMID: 20345943 PMID: 21890895 | Periplasmic | G | N/A | Fimbrium | Periplasmic | fimbriae | SEC | CU | no | yes | Y | 31 | 0.334 | SpI | 30 | 5.91464 | - | 1 | 0 | Y | n15-25c30/31o | Extracellular | 9 | 32 | |||||
| YCBU_ECOLI | P75859 | ycbU | b0942 JW0925 | Fimbrial periplasmic protein (SEC through IM), YcbU | Periplasmic | Potential fimbrial ycb operon (class ??1); Fimbrial Subunit. Ycb operon is homolog to fim operon (ycbv is fimI homolog). | PMID: 20345943 | Periplasmic | G | N/A | Periplasmic | fimbriae | SEC | CU | no | yes | Y | 25 | 0.653 | SpI | 24 | 5.70567 | - | 1 | 0 | Y | n6-17c24/25o | Extracellular | 9 | 41 | |||||||
| YCBV_ECOLI | P75860 | ycbV | b0943 JW5123 | Fimbrial periplasmic protein (SEC through IM), YcbV | Periplasmic | Potential fimbrial ycb operon (class ??1); Fimbrial Subunit. Ycb operon is homolog to fim operon (ycbv is fimI homolog). | PMID: 20345943 | Periplasmic | G | N/A | Periplasmic | fimbriae | SEC | CU | no | yes | N | 23 | 0.301 | SpI | 22 | 5.04607 | - | 0 | 0 | Y | n6-17c22/23o | Unknown | 2 | 47 | |||||||
| GFCD_ECOLI | P75882 | gfcD ymcA | ymcA | b0984 JW0967 | periplasmic protein (SEC through IM), GfcD | Periplasmic | G | N/A | Cell membrane | Periplasmic | SEC | yes | yes | Y | 22 | 0.783 | SpI | 21 | 17.8352 | - | 0 | 0 | Y | n7-15c21/22o | OuterMembrane | 9 | |||||||||||
| GFCC_ECOLI | P75883 | gfcC ymcB | ymcB | b0985 JW0968 | periplasmic protein (SEC through IM), GfcC | Periplasmic | G | N/A | Periplasmic | SEC | yes | yes | Y | 23 | 0.723 | SpI | 22 | 8.45245 | - | 0 | 0 | Y | n4-15c22/23o | Unknown | 2 | 41 | |||||||||||
| GFCB_ECOLI | P75884 | gfcB ymcC | ymcC | b0986 JW0969 | OM lipoprotein (SEC through IM, inserted in OM by LOL), GfcB | Outer Membrane Lipoprotein | Proven pignal peptidase II substrate. PMID: 15174130 | PMID: 15174130 | Outer Membrane Lipoprotein | I | N/A | Cell membrane | Outer membrane Lipoprotein (Experimental) | SEC | LOL | no | yes | Y | 20 | 0.315 | SpII | 15 | 11.1657 | Pos+2=T | 0 | 0 | Y | n4-14c19/20o | CytoplasmicMembrane | 9 | 37 | ||||||
| PGAB_ECOLI | P75906 | pgaB ycdR | ycdR | b1023 JW5142 | OM lipoprotein (SEC through IM), inserted in OM by LOL), Poly-beta-1,6-N-acetyl-D-glucosamine N-deacetylase PgaB | Outer Membrane Lipoprotein | Proven pignal peptidase II substrate. PMID: 15174130 | PMID: 15174130 | Outer Membrane Lipoprotein | I | N/A | Cell outer membrane | Outer membrane Lipoprotein (Experimental) | SEC | LOL | no | yes | N | 26 | 0.161 | SpII | 20 | 14.5441 | Pos+2=I | 0 | 0 | Y | n9-20c25/26o | Periplasmic | 9 | 15 | ||||||
| YMDA_ECOLI | P75917 | ymdA | b1044 JW1031 | periplasmic protein (SEC through IM), YmdA | Periplasmic | G | N/A | Periplasmic | SEC | no | yes | Y | 23 | 0.463 | SpI | 22 | 13.2508 | - | 1 | 0 | Y | n11-22c28/29o | Extracellular | 9 | 36 | ||||||||||||
| FLGA_ECOLI | P75933 | flgA | b1072 JW1059 | periplasmic protein (SEC through IM), Flagella basal body P-ring formation FlgA | Periplasmic | Periplasmic chaperone for P ring protein. PMID: 12730325 | PMID: 12631703 PMID: 12730325 | Periplasmic | G | N/A | Periplasm | Periplasmic (Experimental) | SEC | Fla | yes | yes | Y | 22 | 0.722 | SpI | 21 | 5.00523 | - | 0 | 0 | Y | n8-16c21/22o | Periplasmic | 9 | 12 | |||||||
| FLGD_ECOLI | P75936 | flgD fla FIV flaV | fla FIV flaV | b1075 JW1062 | Flagellar extra-cellular peripheral OM protein (Flagellum secreted), Basal-body rod modification FlgD | Peripheral outer membrane protein facing the extra-cellular space | Hook-capping protein. The hook cap FlgD is displaced by FlgK, allowing filament growth PMID: 12631703 Using the hook scaffolding protein FlgD of Escherichia coli as a model substrate, it is demonstrated that the export signal is contained within the N-terminal 71 amino acids of FlgD. PMID: 16897036 PMID: 16897036 | PMID: 12631703 PMID: 16897036 | Peripheral outer membrane protein facing the extra-cellular space | F4 | N/A | Cell surface appendage (Experimental) | Flagellum | Fla | pseudogene (Ochman & Davalos, 2006) | yes | no | N | 29 | 0.171 | SpI | 28 | 2.5996 | - | 0 | 0 | N | i | Extracellular | 10 | 106 | ||||||
| FLGE_ECOLI | P75937 | flgE fla FV flaK | fla FV flaK | b1076 JW1063 | Flagellar extra-cellular peripheral OM protein (Flagellum secreted), hook FlgE | Peripheral outer membrane protein facing the extra-cellular space | Hook protein. PMID: 12730325 | PMID: 12631703 PMID: 12730325 | Peripheral outer membrane protein facing the extra-cellular space | F4 | N/A | Bacterial flagellum basal body | Cell surface appendage (Experimental) | flagellum | Flagellum | Fla | pseudogene (Ochman & Davalos, 2006) | no | yes | N | 28 | 0.186 | CYT | 0 | -0.200913 | - | 0 | 0 | N | i | Extracellular | 10 | 106 | ||||
| FLGF_ECOLI | P75938 | flgF fla FVI flaX | fla FVI flaX | b1077 JW1064 | Flagellar periplasmic protein (Flagellum secreted through IM), basal body rod FlgF | Periplasmic | Homolog to flgG. Proximal rod protein. PMID: 12730325 FlgB, FlgC, FlgF, and FlgG are four proteins that comprise the rod section of the basal-body assembly of the flagellar motor. The rod is a rotational component which is situated transverse to the outer membrane and peptidoglycan layers and is circumscribed by a set of four rings which lie in a horizontal plane within the inner and outer membranes of the cell envelope. PMID: 2181149 FlgG is a distal component of the basal body rod. While FlgF was highly soluble, FlgB, FlgC, FlgG and FliE tended to self or cross-aggregate into fibrils in solutions at neutral pH or below, at high ionic strength, or at high protein concentration. PMID: 15136044 | PMID: 12631703 PMID: 12730325 PMID: 15136044 PMID: 2181149 | Periplasmic | G | N/A | Bacterial flagellum basal body | Periplasmic (Experimental) | flagellum | Flagellum | Fla | yes | yes | N | 30 | 0.199 | CYT | 0 | -0.200913 | - | 0 | 0 | N | i | Periplasmic | 9 | 101 | |||||
| YCFL_ECOLI | P75946 | ycfL | b1104 JW1090 | OM lipoprotein (SEC through IM, inserted in OM by LOL), YcfL | Outer Membrane Lipoprotein | I | N/A | Outer membrane Lipoprotein | SEC | LOL | no | yes | Y | 24 | 0.214 | SpII | 18 | 19.4253 | Pos+2=R | 0 | 0 | Y | n5-16c21/22o | Unknown | 2 | 70 | |||||||||||
| YCFS_ECOLI | P75954 | ycfS | b1113 JW5820 | periplasmic peripheral OM protein (SEC through IM), Probable L,D-transpeptidase YcfS | Peripheral outer membrane protein facing the periplasm | Potential substrate of periplasmic peptidyl prolyl cis???trans isomerase SurA; sequence contains known binding motif of SurA [RYEF]X[RYEF][P]. YcfS has been shown to crosslink Braun's lipoprotein (Lpp) to the peptidoglycan. | PMID: 18498364 PMID: 23832002 | Peripheral outer membrane protein facing the periplasm | F3 | N/A | Periplasm | Periplasmic | SEC | SURA | yes | yes | Y | 29 | 0.319 | SpI | 28 | 14.2493 | - | 1 | 0 | Y | n11-24c28/29o | Unknown | 2 | 63 | |||||||
| YCGI_ECOLI | P76000 | ycgI ymgH | ymgH | b4521 JW1162/JW5179 b1173 | OM b-barrel protein (SEC through IM), T5S secreted through OM), Putative YcgI | Contains a predicted autotransporter domain. Potential outer membrane b-barrel protein though it does not have prediction for a signal peptide. Contains autotransporter beta-domain <a href="http://www.ebi.ac.uk/interpro/entry/IPR005546/proteins-matched?species=83333">(IPR005546)</a> | Outer Membrane b-barrel protein | H | N/A | Cytoplasmic | SEC | Autotransporter(T5S) | pseudogene (Uniprot), pseudogene (Ochman & Davalos, 2006) | no | no | N | 25 | 0.12 | CYT | 0 | -0.200913 | - | 0 | 0 | N | i | Unknown | 7 | 12 | ||||||||
| YCGJ_ECOLI | P76001 | ycgJ | b1177 JW1166 | periplasmic protein (SEC through IM), YcgJ | Periplasmic | Disulfide bond-containing protein PMID: 25664544 | PMID: 25664544 | Periplasmic | G | N/A | Periplasmic | SEC | yes | yes | Y | 23 | 0.404 | SpI | 22 | 8.58297 | - | 0 | 0 | Y | n6-16c24/25o | Unknown | 2 | 77 | |||||||||
| PLIG_ECOLI | P76002 | pliG ycgK | ycgK | b1178 JW1167 | periplasmic protein (SEC through IM), Inhibitor of g-type lysozyme PliG | Periplasmic | SecB substrate. Potential substrate of periplasmic chaperone YfgM. PMID: 25403562 Putative substrate for the periplasmic chaperone YfgM. PMID: 25403562 | PMID: 16352602 PMID: 20734102 PMID: 25403562 | Periplasmic | G | N/A | Periplasm | Periplasmic | SECB | SEC | no | yes | Y | 23 | 0.798 | SpI | 22 | 19.6754 | - | 0 | 0 | Y | n9-17c22/23o | Unknown | 2 | 72 | ||||||
| YCGV_ECOLI | P76017 | ycgV | b1202 JW1193 | OM b-barrel protein (SEC through IM, T5S secreted through OM), YcgV | Outer Membrane b-barrel protein | Ag43 homologue; known autotransporter protein. Signal peptide 1-56 (by clustalW). Contains autotransporter beta-domain <a href="http://www.ebi.ac.uk/interpro/entry/IPR005546/proteins-matched?species=83333">(IPR005546)</a>. Potential Srp-dependent autotransporter. Homologous to hemoglobin-binding protease hbp. Hbp is the only known autotransporter that is secreted through the integral membrane in a Srp-dependent manner. | PMID: 12466262 PMID: 15590781 | Outer Membrane b-barrel protein | H | IM (predicted) | Cytoplasmic | SRP | SEC | Autotransporter(T5S) | no | yes | N | 47 | 0.309 | CYT | 0 | -0.200913 | - | 0 | 1 | N | i27-47o | Extracellular | 9 | 17 | |||||||
| YCJN_ECOLI | P76042 | ycjN | b1310 JW1303 | OM lipoprotein (SEC through IM), inserted in OM by LOL), Putative ABC transporter periplasmic-binding YcjN | Outer Membrane Lipoprotein | Phobius prediction hydrophobic region around residue 400 with transmembrane probability equal to 0.8. Minor prediction of weak transmembrane region in the same region by TMHMM . Otherwise no obvious signals. Homologous to P28904 (TREC_ECOLI) Trehalose-6-phosphate hydrolase that is totally soluble and cytoplasmic Wikipedia classifies Categories: Transferases | EC 2.4.1 | Peripheral membrane proteins. In Streptococcus Jaecalis it is not found associated with the membrane. Proven pignal peptidase II substrate. PMID: 15174130 | PMID: 13859056 PMID: 15174130 PMID: 15660210 | Outer Membrane Lipoprotein | I | N/A | Periplasm | Outer membrane Lipoprotein (Experimental) | SEC | LOL | no | yes | N | 20 | 0.189 | SpII | 20 | 13.8019 | Pos+2=K | 0 | 0 | Y | n6-17c21/22o | Unknown | 2 | 66 | |||||||
| OMPG_ECOLI | P76045 | ompG | b1319 JW1312 | OM b-barrel protein (SEC through IM, inserted in OM by BAM), OmpG | Outer Membrane b-barrel protein | Proteoliposome swelling assays showed that OmpG channels were much larger than those of the OmpF and OmpC porins, with an estimated limited diameter of about 2 nm. PMID: 9721282 Potential Skp substrate PMID: 19953543 PMID: 9721282 | PMID: 19953543 PMID: 9721282 | Outer Membrane b-barrel protein | H | N/A | Cell outer membrane; Multi-pass membrane protein | Outer Membrane ??-barrel protein (Experimental) | SEC | SKP | BAM | no | yes | Y | 20 | 0.465 | SpI | 21 | 14.867 | - | 0 | 0 | Y | n4-14c19/20o | OuterMembrane | 10 | 54 | ||||||
| YDBL_ECOLI | P76076 | ydbL | b1383 JW5216 | periplasmic protein (SEC through IM), YdbL | Periplasmic | G | N/A | Periplasmic | SEC | yes | yes | Y | 22 | 0.838 | SpI | 21 | 15.5115 | - | 1 | 0 | Y | n4-15c21/22o | Unknown | 2 | 51 | ||||||||||||
| YDCS_ECOLI | P76108 | ydcS | b1440 JW1435 | periplasmic protein (SEC through IM), Bifunctional polyhydroxybutyrate synthase / ABC transporter periplasmic binding YdcS | Periplasmic | Potential substrate for the SRP pathway. It was found to be down-regulated in the periplasmic compartment of SRP-depleted cells. PMID: 22030350 Disulfide bond-containing protein PMID: 25664544 | PMID: 15911532 PMID: 22030350 PMID: 25664544 | Periplasmic | G | N/A | Periplasm | Periplasmic (Experimental) | SEC | no | yes | Y | 23 | 0.836 | SpI | 22 | 17.8592 | - | 0 | 0 | Y | n10-18c22/23o | Periplasmic | 10 | 16 | ||||||||
| YNCD_ECOLI | P76115 | yncD | b1451 JW1446 | OM b-barrel protein (SEC through IM, inserted in OM by BAM), Probable TonB-dependent receptor YncD | Outer Membrane b-barrel protein | Outer membrane protein determined by 2D gel electrophoresis (Lopez-Campistrous et al 2005, Fountoulakis et al 2003) Potential Skp substrate PMID: 19953543 | PMID: 12624733 PMID: 19953543 PMID: 20932056 | Outer Membrane b-barrel protein | H | N/A | Cell outer membrane | Outer Membrane ??-barrel protein | SEC | SKP | BAM | no | yes | Y | 24 | 0.888 | SpI | 23 | 12.8703 | - | 0 | 0 | Y | n10-18c23/24o | OuterMembrane | 10 | 2 | ||||||
| YNCE_ECOLI | P76116 | yncE | b1452 JW1447 | periplasmic protein (SEC through IM), YncE | Periplasmic | SecB substrate. | PMID: 16352602 | Periplasmic | G | N/A | Periplasmic | SECB | SEC | no | yes | Y | 31 | 0.794 | SpI | 30 | 16.4204 | - | 0 | 0 | Y | n15-26c30/31o | Unknown | 2 | 64 | ||||||||
| YNCH_ECOLI | P76118 | yncH | b1455 JW5235 | periplasmic protein (SEC through IM), YncH | Periplasmic | G | IM (predicted) | Cytoplasmic | SEC | no | yes | N | 40 | 0.113 | CYT | 0 | -0.200913 | - | 0 | 0 | Y | n2-13c18/19o | Unknown | 2 | 35 | ||||||||||||
| DDPA_ECOLI | P76128 | ddpA yddS | yddS | b1487 JW5240 | periplasmic protein (SEC through IM), Probable D,D-dipeptide-binding periplasmic DdpA | Periplasmic | PMID: 11121068 | Periplasmic | G | N/A | Periplasm | Periplasmic (Experimental) | SEC | no | yes | Y | 26 | 0.839 | SpI | 25 | 19.0002 | - | 0 | 0 | Y | n10-17c25/26o | Periplasmic | 10 | 61 | ||||||||
| LSRB_ECOLI | P76142 | lsrB yneA | yneA | b1516 JW1509 | periplasmic protein (SEC through IM), Autoinducer 2-binding LsrB | Periplasmic | PMID: 15601708 | Periplasmic | G | N/A | Periplasm | Periplasmic (Experimental) | SEC | no | yes | Y | 27 | 0.845 | SpI | 26 | 21.9126 | - | 0 | 0 | Y | n9-20c26/27o | Periplasmic | 10 | 75 | ||||||||
| RZPQ_ECOLI | P76158 | rzpQ ydfP | ydfP | b1553 JW1545 | periplasmic protein (SEC through IM), RzpQ | Periplasmic | G | N/A | Periplasmic | SEC | Prophage Integrase (EcoGene) | no | no | N | 23 | 0.245 | SpI | 22 | 0.491707 | - | 1 | 0 | Y | n7-17c22/23o | Unknown | 2 | 19 | ||||||||||
| YNFB_ECOLI | P76170 | ynfB | b1583 JW1575 | periplasmic peripheral OM protein (SEC through IM), UPF0482 YnfB | Peripheral outer membrane protein facing the periplasm | Outer membrane protein determined by 2D gel electrophoresis (Lopez-Campistrous et al 2005) Disulfide bond-containing protein PMID: 25664544 | PMID: 15911532 PMID: 25664544 | Peripheral outer membrane protein facing the periplasm | F3 | N/A | Periplasmic | SEC | yes | yes | Y | 29 | 0.87 | SpI | 28 | 19.5483 | - | 0 | 0 | Y | n9-20c28/29o | Unknown | 2 | 49 | |||||||||
| YNFD_ECOLI | P76172 | ynfD | b1586 JW5259 | periplasmic protein (SEC through IM), YnfD | Periplasmic | Experimentally verified gene-product. PMID: 9868784 | PMID: 9868784 | Periplasmic | G | Soluble IM Fraction | Periplasmic (Experimental) | SEC | no | yes | Y | 22 | 0.874 | SpI | 21 | 22.7125 | - | 0 | 0 | Y | n3-14c21/22o | Extracellular | 9 | 79 | |||||||||
| YDGD_ECOLI | P76176 | ydgD | b1598 JW1590 | periplasmic (SEC through IM) serine protease YdgD | Periplasmic | G | N/A | Periplasmic | SEC | yes | yes | Y | 22 | 0.691 | SpI | 21 | 15.015 | - | 0 | 0 | Y | n3-14c21/22o | Unknown | 2 | 28 | ||||||||||||
| YDGH_ECOLI | P76177 | ydgH | b1604 JW1596 | periplasmic protein (SEC through IM), YdgH | Periplasmic | G | N/A | Periplasmic | SEC | yes | yes | Y | 23 | 0.805 | SpI | 22 | 22.3224 | - | 0 | 0 | Y | n7-18c22/23o | Unknown | 2 | 13 | ||||||||||||
| MEPH_ECOLI | P76190 | mepH ydhO | ydhO | b1655 JW5270 | periplasmic protein (SEC through IM), Murein DD-endopeptidase MepH | Periplasmic | G | N/A | Periplasmic | SEC | yes | yes | Y | 28 | 0.421 | SpI | 27 | 15.3587 | - | 0 | 0 | Y | n8-19c27/28o | Unknown | 6 | ||||||||||||
| YNHG_ECOLI | P76193 | ynhG | b1678 JW1668 | periplasmic peripheral OM protein (SEC through IM), Probable L,D-transpeptidase YnhG | Peripheral outer membrane protein facing the periplasm | Potential substrate of periplasmic peptidyl prolyl cis???trans isomerase SurA; sequence contains known binding motif of SurA [RYEF]X[RYEF][P]. YnhG forms direct meso- diaminopimelate (DAP-DAP, or 3-3) crosslinks within the peptidoglycan. | PMID: 18498364 PMID: 23832002 | Peripheral outer membrane protein facing the periplasm | F3 | N/A | Periplasm | Periplasmic | SEC | SURA | yes | yes | Y | 24 | 0.48 | SpI | 23 | 15.3547 | - | 0 | 0 | Y | n4-15c23/24o | Periplasmic | 9 | 22 | |||||||
| YDIY_ECOLI | P76206 | ydiY | b1722 JW1711 | periplasmic protein (SEC through IM), YdiY | Periplasmic | G | N/A | Periplasmic | SEC | yes | yes | Y | 26 | 0.481 | SpI | 25 | 20.5374 | - | 0 | 0 | Y | n7-18c23/24o | Unknown | 2 | 15 | ||||||||||||
| YDJY_ECOLI | P76220 | ydjY | b1751 JW5283 | IM lipoprotein (SEC through IM, inserted in OM by LOL), YdjY | Inner Membrane Lipoprotein | Inner Membrane Lipoprotein | E | Soluble IM Fraction | Inner membrane lipoprotein | SEC | LOL | no | yes | N | 34 | 0.346 | SpII | 26 | 15.9526 | Pos+2=D | 0 | 0 | Y | n13-24c29/30o | Unknown | 2 | 96 | ||||||||||
| YNJB_ECOLI | P76223 | ynjB | b1754 JW5284 | periplasmic protein (SEC through IM), YnjB | Periplasmic | Potential substrate of periplasmic peptidyl prolyl cis???trans isomerase SurA; sequence contains known binding motif of SurA [RYEF]X[RYEF][P]. | PMID: 18498364 | Periplasmic | G | N/A | Periplasmic | SEC | SURA | no | yes | Y | 21 | 0.688 | SpI | 20 | 11.9034 | - | 0 | 0 | Y | n4-16c20/21o | Unknown | 2 | 70 | ||||||||
| YNJH_ECOLI | P76227 | ynjH | b1760 JW1749 | periplasmic protein (SEC through IM), YnjH | Periplasmic | Disulfide bond-containing protein PMID: 25664544 | PMID: 25664544 | Periplasmic | G | N/A | Periplasmic | SEC | no | yes | Y | 19 | 0.569 | SpI | 18 | 11.8884 | - | 0 | 0 | Y | n4-14c18/19o | CytoplasmicMembrane | 9 | 27 | |||||||||
| YECT_ECOLI | P76296 | yecT | b1877 JW5310 | periplasmic protein (SEC through IM), YecT | Periplasmic | G | N/A | Periplasmic | SEC | no | yes | Y | 19 | 0.831 | SpI | 18 | 11.1311 | - | 0 | 0 | Y | n4-14c18/19o | Unknown | 4 | 31 | ||||||||||||
| FLHE_ECOLI | P76297 | flhE | b1878 JW1867 | periplasmic protein (SEC through IM), FlhE | Periplasmic | Part of the flagellar export apparatus Disulfide bond-containing protein PMID: 25664544 | PMID: 25664544 | Periplasmic | G | N/A | Periplasmic | SEC | Fla | yes | yes | Y | 17 | 0.511 | SpI | 16 | 8.24229 | - | 0 | 0 | Y | n3-14c26/27o | Unknown | 2 | 68 | ||||||||
| YECR_ECOLI | P76308 | yecR | b1904 JW1892 | OM lipoprotein (SEC through IM, inserted in OM by LOL), YecR | Outer Membrane Lipoprotein | Proven pignal peptidase II substrate. PMID: 15174130 | PMID: 15174130 | Outer Membrane Lipoprotein | I | N/A | Outer membrane Lipoprotein (Experimental) | SEC | LOL | no | yes | N | 25 | 0.325 | SpII | 15 | 8.98675 | Pos+2=T | 0 | 0 | Y | n3-14c22/23o | Unknown | 2 | 15 | ||||||||
| YEDS_ECOLI | P76335 | yedS | b4496 JW5319/JW1948/JW1949 b1964/b1965/b1966 | OM b-barrel protein (SEC through IM, inserted in OM by BAM), Putative YedS | Outer Membrane b-barrel protein | H | N/A | Cell outer membrane | Cytoplasmic | SEC | BAM | pseudogene (Uniprot), pseudogene (Ochman & Davalos, 2006) | yes | no | Y | 22 | 0.864 | SpI | 21 | 22.1485 | - | 0 | 0 | Y | n5-16c21/22o | OuterMembrane | 10 | 18 | |||||||||
| HIUH_ECOLI | P76341 | hiuH yedX | yedX | b1970 JW1953 | periplasmic protein (SEC through IM), 5-hydroxyisourate hydrolase HiuH | Periplasmic | PMID: 12542701 | Periplasmic | G | N/A | Periplasm | Periplasmic | SEC | yes | yes | Y | 24 | 0.79 | SpI | 23 | 18.1944 | - | 0 | 0 | Y | n6-17c23/24o | Unknown | 2 | 78 | ||||||||
| MSRP_ECOLI | P76342 | msrP yedY | yedY | b1971 JW1954 | periplasmic peripheral IM protein (TAT-SEC through IM), Protein-methionine-sulfoxide reductase catalytic subunit MsrP | Peripheral inner membrane protein facing the periplasm | TAT secretion system. PMID: 15355966 | PMID: 15355966 PMID: 16042411 PMID: 17218314 | Peripheral inner membrane protein facing the periplasm | F2 | N/A | Periplasm | Membrane anchored (Experimental) | TAT,SEC | no | yes | N | 45 | 0.526 | SpI | 44 | 7.04958 | - | 0 | 0 | Y | n28-38c44/45o | Unknown | 2 | 7 | |||||||
| ZINT_ECOLI | P76344 | zinT yodA | yodA | b1973 JW1956 | periplasmic protein (SEC through IM), Metal-binding ZinT | Periplasmic | After 45 min of cadmium exposure the YodA protein was primarily detected in the cytoplasmic fraction but was later (150 min) found in both the cytoplasmic and periplasmic compartments. PMID: 9579078 Disulfide bond-containing protein PMID: 25664544 | PMID: 12480884 PMID: 25664544 PMID: 9579078 | Periplasmic | G | N/A | Cytoplasm | Periplasmic (Experimental) | SEC | yes | yes | Y | 24 | 0.683 | SpI | 23 | 12.2997 | - | 0 | 0 | Y | n8-18c23/24o | Unknown | 2 | ||||||||
| YEEJ_ECOLI | P76347 | yeeJ | b1978 JW5833 | OM b-barrel protein (SEC through IM, inserted in OM by BAM), YeeJ | Outer Membrane b-barrel protein | Cytplasmic transciption factor that shuttles from chromosome to the membranes as a peripheral protein on the PtsG transporter. Specifically it binds to the unphosphorylated, tRNAs port-mediating form of the EIIBCGl subunit. | PMID: 15063852 | Outer Membrane b-barrel protein | H | N/A | Cytoplasmic | SEC | BAM | no | yes | N | 52 | 0.258 | SpI | 45 | 1.97486 | - | 0 | 0 | Y | n27-39c51/52o | OuterMembrane | 9 | |||||||||
| YOEA_ECOLI | P76356 | yoeA | b4582 JW1980 b1995 b1998 | OM b-barrel protein (SEC through IM, inserted in OM by BAM), Putative YoeA | Outer Membrane b-barrel protein | H | N/A | Outer Membrane ??-barrel protein | SEC | BAM | pseudogene (Uniprot), pseudogene (Ochman & Davalos, 2006) | no | no | Y | 29 | 0.402 | SpI | 28 | 8.15876 | - | 0 | 0 | Y | n12-23c28/29o | OuterMembrane | 9 | |||||||||||
| YEGJ_ECOLI | P76394 | yegJ | b2071 JW2056 | periplasmic protein (SEC through IM), YegJ | Periplasmic | G | N/A | Periplasmic | SEC | no | yes | Y | 19 | 0.264 | SpI | 19 | 9.75379 | - | 0 | 0 | Y | n4-14c19/20o | Unknown | 2 | 79 | ||||||||||||
| MDTA_ECOLI | P76397 | mdtA yegM | yegM | b2074 JW5338 | periplasmic peripheral IM protein (SEC through IM), Multidrug resistance MdtA | Peripheral inner membrane protein facing the periplasm | Part of MdtABC-TolC multidrug efflux transport system = [(TolC)3][MdtC][MdtB][MdtA]. | PMID: 12107133 PMID: 12107134 PMID: 21890895 | Peripheral inner membrane protein facing the periplasm | F2 | N/A | Cell inner membrane | Membrane anchored | SEC | yes | yes | N | 22 | 0.151 | TMH | 0 | 7.31567 | - | 1 | 1 | N | i9-26o | CytoplasmicMembrane | 9 | 37 | |||||||
| YEGR_ECOLI | P76406 | yegR | b2085 JW5837 | OM lipoprotein (SEC through IM, inserted in OM by LOL), YegR | Outer Membrane Lipoprotein | I | N/A | Outer membrane Lipoprotein | SEC | LOL | yes | yes | Y | 22 | 0.281 | SpII | 17 | 15.1742 | Pos+2=A | 0 | 0 | Y | n4-14c22/23o | Unknown | 2 | 54 | |||||||||||
| YFAP_ECOLI | P76462 | yfaP | b2225 JW2219 | periplasmic protein (SEC through IM), YfaP | Periplasmic | Potential substrate of periplasmic peptidyl prolyl cis???trans isomerase SurA; sequence contains known binding motif of SurA [RYEF]X[RYEF][P]. | PMID: 18498364 | Periplasmic | G | N/A | Periplasmic | SEC | SURA | yes | yes | Y | 20 | 0.599 | SpI | 19 | 13.0156 | - | 0 | 0 | Y | n4-14c19/20o | Unknown | 2 | 107 | ||||||||
| YFAQ_ECOLI | P76463 | yfaQ | b2226 JW2220 | periplasmic protein (SEC through IM), YfaQ | Periplasmic | Potential substrate of periplasmic peptidyl prolyl cis???trans isomerase SurA; sequence contains known binding motif of SurA [RYEF]X[RYEF][P]. | PMID: 18498364 | Periplasmic | G | N/A | Periplasmic | SEC | SURA | no | yes | Y | 20 | 0.66 | SpI | 19 | 8.15864 | - | 0 | 0 | Y | n6-15c19/20o | Unknown | 2 | 9 | ||||||||
| YFAS_ECOLI | P76464 | yfaS yfaR | yfaR | b4500 JW2221/JW2222 b2227/b2228 | periplasmic protein (SEC through IM), Putative alpha-2-macroglobulin homolog YfaS | Periplasmic | Potential substrate of periplasmic peptidyl prolyl cis???trans isomerase SurA; sequence contains known binding motif of SurA [RYEF]X[RYEF][P]. | PMID: 18498364 | Periplasmic | G | Soluble IM Fraction | Periplasmic | SEC | SURA | pseudogene (Ochman & Davalos, 2006), pseudogene (Uniprot) | no | yes | Y | 39 | 0.71 | SpI | 38 | 14.0209 | - | 0 | 0 | Y | n20-31c38/39o | Unknown | 4 | 81 | ||||||
| YFAT_ECOLI | P76466 | yfaT | b2229 JW2223 | periplasmic protein (SEC through IM), YfaT | Periplasmic | G | N/A | Periplasmic | SEC | no | yes | Y | 20 | 0.277 | SpI | 19 | 3.99575 | - | 0 | 0 | Y | n4-15c19/20o | CytoplasmicMembrane | 9 | 26 | ||||||||||||
| YFAZ_ECOLI | P76471 | yfaZ | b2250 JW5371 | OM b-barrel protein (SEC through IM, inserted in OM by BAM), YfaZ | Outer Membrane b-barrel protein | PMID: 20932056 | Outer Membrane b-barrel protein | H | N/A | Cell outer membrane | Outer Membrane ??-barrel protein | SEC | BAM | no | yes | Y | 22 | 0.713 | SpI | 21 | 19.9632 | - | 0 | 0 | Y | n4-15c21/22o | Unknown | 2 | 78 | ||||||||
| YFBK_ECOLI | P76481 | yfbK | b2270 JW2265 | IM lipoprotein (SEC through IM, inserted in OM by LOL), YfbK | Inner Membrane Lipoprotein | E | N/A | Membrane Lipoprotein | SEC | LOL | no | yes | N | 24 | 0.119 | SpII | 18 | 27.352 | Pos+2=G | 0 | 0 | Y | n6-16c21/22o | Unknown | 2 | 49 | |||||||||||
| YFCO_ECOLI | P76498 | yfcO | b2332 JW2329 | Fimbrial periplasmic protein (SEC through IM), YfcO | Periplasmic | Putative chaperone???usher fimbrial operon yfc (class ??); Unkown function | PMID: 20345943 | Periplasmic | G | Soluble IM Fraction | Periplasmic | fimbriae | SEC | no | yes | Y | 22 | 0.835 | SpI | 21 | 14.5584 | - | 0 | 0 | Y | n6-16c21/22o | Extracellular | 9 | 41 | ||||||||
| YFCP_ECOLI | P76499 | yfcP | b2333 JW2330 | Fimbrial periplasmic protein (SEC through IM), YfcP | Periplasmic | Putative chaperone???usher fimbrial operon yfc (class ??); Fimbrial Subunit. Disulfide bridge (43 83) | PMID: 19820722 PMID: 20345943 | Periplasmic | G | N/A | Fimbrium | Periplasmic | fimbriae | SEC | CU | yes | yes | Y | 28 | 0.79 | SpI | 27 | 14.214 | - | 1 | 0 | Y | n8-19c27/28o | Unknown | 2 | |||||||
| YFCQ_ECOLI | P76500 | yfcQ | b2334 JW2331 | Fimbrial periplasmic protein (SEC through IM), YfcQ | Periplasmic | Putative chaperone???usher fimbrial operon yfc (class ??); Fimbrial Subunit. | PMID: 20345943 | Periplasmic | G | N/A | Periplasmic | fimbriae | SEC | yes | yes | Y | 19 | 0.841 | SpI | 18 | 13.6429 | - | 0 | 0 | Y | n4-11c18/19o | Unknown | 2 | 96 | ||||||||
| YFCR_ECOLI | P76501 | yfcR | b2335 JW2332 | Fimbrial periplasmic protein (SEC through IM), YfcR | Periplasmic | Putative chaperone???usher fimbrial operon yfc (class ??); Fimbrial Subunit. | PMID: 20345943 | Periplasmic | G | N/A | Periplasmic | fimbriae | SEC | yes | yes | Y | 29 | 0.68 | SpI | 28 | 11.4125 | - | 0 | 0 | Y | n10-22c30/31o | Unknown | 2 | 49 | ||||||||
| MLAA_ECOLI | P76506 | mlaA vacJ | vacJ | b2346 JW2343 | OM lipoprotein (SEC through IM), inserted in OM by LOL), Interphospholipid transport system MlaA | Outer Membrane Lipoprotein | Proven pignal peptidase II substrate. PMID: 15174130 | PMID: 15174130 PMID: 20932056 | Outer Membrane Lipoprotein | I | N/A | Cell outer membrane | Outer membrane Lipoprotein (Experimental) | SEC | LOL | yes | yes | Y | 22 | 0.16 | SpII | 17 | 20.7085 | Pos+2=A | 0 | 0 | Y | n5-16c21/22o | OuterMembrane | 9 | 19 | ||||||
| YFDX_ECOLI | P76520 | yfdX | b2375 JW2372 | periplasmic protein (SEC through IM), YfdX | Periplasmic | PMID: 11157960 | Periplasmic | G | N/A | Periplasmic (Experimental) | SEC | yes | yes | Y | 22 | 0.881 | SpI | 21 | 20.5809 | - | 0 | 0 | Y | n4-15c21/22o | Unknown | 2 | 49 | ||||||||||
| YFEY_ECOLI | P76537 | yfeY | b2432 JW2425 | OM lipoprotein (SEC through IM, inserted in OM by LOL), YfeY | Outer Membrane Lipoprotein | Proven pignal peptidase II substrate. Outer membrane protein determined by LC-MS (Gevaert et al 2002) PMID: 15174130 Disulfide bond-containing protein PMID: 25664544 | PMID: 12488465 PMID: 15174130 PMID: 20932056 PMID: 25664544 | Outer Membrane Lipoprotein | I | N/A | Outer membrane Lipoprotein (Experimental) | SEC | LOL | yes | yes | Y | 29 | 0.248 | SpII | 17 | 20.3796 | Pos+2=S | 0 | 0 | Y | n8-19c27/28o | Unknown | 2 | 59 | ||||||||
| YFFQ_ECOLI | P76548 | yffQ | b2448 | periplasmic protein (SEC through IM), YffQ | Periplasmic | G | N/A | Periplasmic | SEC | Prophage Integrase (EcoGene) | no | no | N | 19 | 0.652 | SpI | 18 | 3.21713 | - | 0 | 0 | Y | n3-13c18/19o | Unknown | 4 | ||||||||||||
| YPFG_ECOLI | P76559 | ypfG | b2466 JW2450 | periplasmic protein (SEC through IM), YpfG | Periplasmic | Disulfide bond-containing protein PMID: 25664544 | PMID: 25664544 | Periplasmic | G | N/A | Periplasmic | SEC | yes | yes | Y | 22 | 0.804 | SpI | 21 | 15.2807 | - | 1 | 0 | Y | n5-16c21/22o | CytoplasmicMembrane | 9 | 61 | |||||||||
| YFGI_ECOLI | P76573 | yfgI | b2506 JW2490 | periplasmic protein (SEC through IM), YfgI | Periplasmic | G | N/A | Periplasmic | SEC | no | yes | Y | 20 | 0.625 | SpI | 19 | 7.20342 | - | 0 | 0 | Y | n4-14c32/33o | Unknown | 2 | 11 | ||||||||||||
| YFHM_ECOLI | P76578 | yfhM | b2520 JW2504 | IM lipoprotein (SEC through IM), inserted in OM by LOL), Alpha-2-macroglobulin YfhM | Inner Membrane Lipoprotein | Identified DsbA substrate PMID: 19953543 | PMID: 19953543 | Inner Membrane Lipoprotein | E | Soluble IM Fraction | Cell inner membrane | Inner membrane lipoprotein | SEC | DSBA | LOL | yes | yes | Y | 25 | 0.18 | SpII | 17 | 28.7232 | Pos+2=D | 0 | 0 | Y | n6-13c18/19o | Unknown | 4 | |||||||
| YQIG_ECOLI | P76655 | yqiG | b3046 JW5507 | Fimbrial OM b-barrel protein (SEC through IM, inserted in OM by BAM), Putative usher YqiG | Outer Membrane b-barrel protein | Putative chaperone???usher fimbrial operon (class ??); Usher. Disulfide bridge (798 817) | PMID: 20345943 | Outer Membrane b-barrel protein | H | N/A | Cell outer membrane | Outer Membrane ??-barrel protein | fimbriae | SEC | BAM | pseudogene (Uniprot) | no | yes | N | 21 | 0.333 | CYT | 0 | -0.200913 | - | 0 | 0 | N | i | OuterMembrane | 10 | ||||||
| YQII_ECOLI | P76656 | yqiI | b3048 JW5509 | Fimbrial periplasmic protein (SEC through IM), YqiI | Periplasmic | Putative chaperone???usher fimbrial operon (class ??); Unknown function. | PMID: 20345943 | Periplasmic | G | N/A | Periplasmic | fimbriae | SEC | no | yes | Y | 22 | 0.868 | SpI | 21 | 14.4478 | - | 0 | 0 | Y | n4-14c21/22o | Unknown | 2 | 85 | ||||||||
| OMPL_ECOLI | P76773 | ompL yshA | yshA | b3875 JW3846 | OM b-barrel protein (SEC through IM, inserted in OM by BAM), Porin OmpL | Outer Membrane b-barrel protein | PMID: 11080145 | Outer Membrane b-barrel protein | H | N/A | Cell outer membrane | Outer Membrane ??-barrel protein (Experimental) | SEC | BAM | no | yes | Y | 21 | 0.672 | SpI | 20 | 13.4725 | - | 0 | 0 | Y | n6-16c20/21o | OuterMembrane | 10 | 6 | |||||||
| YKFB_ECOLI | P77162 | ykfB | b0250 JW0239 | periplasmic protein (SEC through IM), YkfB | Periplasmic | Disulfide bond-containing protein PMID: 25664544 | PMID: 25664544 | Periplasmic | G | N/A | Periplasmic | SEC | Prophage Integrase (EcoGene) | no | no | Y | 24 | 0.716 | SpI | 23 | 17.8556 | - | 0 | 0 | Y | n11-19c23/24o | Unknown | 2 | 59 | ||||||||
| YAGT_ECOLI | P77165 | yagT | b0286 JW0280 | periplasmic protein (TAT through IM), Aldehyde oxidoreductase iron-sulfur-binding subunit YagT | Periplasmic | TAT secretion system. YagT protein contains a 49 amino acid Tat leader peptide that allows the export of the active heterotrimer to the periplasm. Tat substrates are matured in the cytoplasm prior to their translocation. PMID: 19368556 | PMID: 17218314 PMID: 19368556 | Periplasmic | G | N/A | Periplasm | Cytoplasmic | TAT | no | yes | N | 49 | 0.297 | CYT | 0 | -0.200913 | - | 0 | 0 | N | i | Unknown | 4 | 89 | ||||||||
| LOMR_ECOLI | P77184 | lomR | b4570 JW5884/JW5904 b1369/b1371 | OM b-barrel protein (SEC through IM), Putative LomR | Outer Membrane b-barrel protein | H | N/A | Cytoplasmic | SEC | Insertion Sequence, pseudogene (Uniprot), pseudogene (Ochman & Davalos, 2006) | no | no | N | 27 | 0.169 | SpI | 26 | 0.733275 | - | 0 | 0 | N | i | OuterMembrane | 10 | ||||||||||||
| ECPB_ECOLI | P77188 | ecpB matC yagY | matC yagY | b0292 JW0286 | Fimbrial periplasmic protein (SEC through IM), Probable chaperone EcpB | Periplasmic | Putative chaperone???usher fimbrial operon yag(class ??); Unknown function. | PMID: 20345943 | Periplasmic | G | N/A | Periplasmic | fimbriae | SEC | no | yes | Y | 21 | 0.828 | SpI | 20 | 12.558 | - | 0 | 0 | Y | n5-15c20/21o | Unknown | 2 | 25 | |||||||
| YFCU_ECOLI | P77196 | yfcU yfcT | yfcT | b4661 JW2334/JW2335 b2337 b2338 | Fimbrial OM b-barrel protein (SEC through IM), Putative usher YfcU | Outer Membrane b-barrel protein | H | N/A | Cell outer membrane | Cytoplasmic | fimbriae | SEC | pseudogene (Ochman & Davalos, 2006), pseudogene (Uniprot) | no | yes | Y | 30 | 0.624 | SpI | 29 | 5.84101 | - | 0 | 0 | Y | n11-21c29/30o | OuterMembrane | 10 | 79 | ||||||||
| YAIT_ECOLI | P77199 | yaiT | b4580 JW0362/JW5051 b0371 b0374 | OM b-barrel protein (SEC through IM), T5S secreted through OM), Putative YaiT | Outer Membrane b-barrel protein | Ag43 homologue; predicted autotransporter. Contains autotransporter beta-domain <a href="http://www.ebi.ac.uk/interpro/entry/IPR005546/proteins-matched?species=83333">(IPR005546)</a>. Potential Srp-dependent autotransporter. Homologous to hemoglobin-binding protease hbp. Hbp is the only known autotransporter that is secreted through the integral membrane in a Srp-dependent manner. | PMID: 12466262 PMID: 15590781 | Outer Membrane b-barrel protein | H | N/A | Periplasmic | SRP | SEC | Autotransporter(T5S) | pseudogene (Uniprot), pseudogene (Ochman & Davalos, 2006) | no | no | Y | 28 | 0.844 | SpI | 27 | 23.6574 | - | 0 | 0 | Y | n8-19c27/28o | Unknown | 5 | 55 | ||||||
| DSBG_ECOLI | P77202 | dsbG ybdP | ybdP | b0604 JW0597 | periplasmic protein (SEC through IM), Thiol:disulfide interchange DsbG | Periplasmic | Disulfide bridge (126 129) PMID: 15184683 Disulfide bond-containing protein PMID: 25664544 | PMID: 15184683 PMID: 25664544 PMID: 9383195 | Periplasmic | G | N/A | Periplasm | Periplasmic (Experimental) | SEC | yes | yes | Y | 18 | 0.618 | SpI | 17 | 12.1105 | - | 0 | 0 | Y | n5-12c17/18o | Periplasmic | 10 | 50 | |||||||
| CUSC_ECOLI | P77211 | cusC ibeB ylcB | ibeB ylcB | b0572 JW0561 | OM lipoprotein (SEC through IM), inserted in OM by LOL), Cation efflux system CusC | Outer Membrane Lipoprotein | Proven pignal peptidase II substrate. PMID: 15174130 | PMID: 15174130 PMID: 20932056 PMID: 21249122 | Outer Membrane Lipoprotein | I | N/A | Cell outer membrane | Outer membrane Lipoprotein (Experimental) | SEC | LOL | yes | yes | Y | 22 | 0.405 | SpII | 17 | 11.8848 | Pos+2=S | 0 | 0 | Y | n6-15c20/21o | OuterMembrane | 10 | 27 | ||||||
| CUSF_ECOLI | P77214 | cusF cusX ylcC | cusX ylcC | b0573 JW0562 | periplasmic protein (SEC through IM), Cation efflux system CusF | Periplasmic | PMID: 12813074 | Periplasmic | G | N/A | Periplasm | Periplasmic (Experimental) | SEC | yes | yes | Y | 23 | 0.444 | SpI | 22 | 8.68332 | - | 0 | 0 | Y | n4-15c22/23o | Periplasmic | 10 | |||||||||
| CUSB_ECOLI | P77239 | cusB ylcD | ylcD | b0574 JW0563 | periplasmic peripheral IM protein (SEC through IM), Cation efflux system CusB | Peripheral inner membrane protein facing the periplasm | MFP family function as auxiliary proteins or 'adaptors', connecting a primary porter in the cytoplasmic membrane of a Gram-negative bacterium with an outer membrane factor (OMF; TC #1.B.17) protein that serves a porin or channel function in the outer membrane. | PMID: 19695261 | Peripheral inner membrane protein facing the periplasm | F2 | N/A | Membrane anchored | SEC | yes | yes | N | 29 | 0.243 | SpI | 28 | 7.5302 | - | 1 | 0 | Y | n4-16c28/29o | Unknown | 2 | 56 | ||||||||
| SFMC_ECOLI | P77249 | sfmC | b0531 JW0520 | Fimbrial periplasmic protein (SEC through IM), Probable chaperone SfmC | Periplasmic | Potential fimbrial sfm operon (class ??1); Chaperone. Potential substrate of periplasmic peptidyl prolyl cis???trans isomerase SurA; sequence contains known binding motif of SurA [RYEF]X[RYEF][P]. Might be is co-translationally translocated through Sec translocon. The precursor of SfmC harbouring hydrophobic signal sequence require SRP and FtsY in addition to SecA for maximal translocation. PMID: 24717922 The precursor of SfmC harbouring hydrophobic signal sequence require SRP and FtsY in addition to SecA for maximal translocation. PMID: 24717922 Potential substrate for the SRP pathway. When fused to cytoplasmic protein thioredoxin promotes signal recognition particle-dependent translocation PMID: 15838024 | PMID: 15838024 PMID: 18498364 PMID: 20345943 PMID: 24717922 | Periplasmic | G | N/A | Periplasm | Periplasmic | fimbriae | SRP (exp) | SEC | SURA | yes | yes | Y | 24 | 0.844 | SpI | 23 | 14.9715 | - | 1 | 0 | Y | n7-18c23/24o | Periplasmic | 10 | 28 | |||||
| ECPE_ECOLI | P77263 | ecpE matF yagV | matF yagV | b0289 JW5030 | Fimbrial periplasmic protein (SEC through IM), Probable chaperone EcpE | Periplasmic | Putative chaperone???usher fimbrial operon yag(class ??); Unknown function. Potential substrate of periplasmic peptidyl prolyl cis???trans isomerase SurA; sequence contains known binding motif of SurA [RYEF]X[RYEF][P]. Disulfide bond-containing protein PMID: 25664544 | PMID: 18498364 PMID: 20345943 PMID: 25664544 | Periplasmic | G | N/A | Periplasmic | fimbriae | SEC | SURA | no | yes | Y | 28 | 0.534 | SpI | 27 | 8.52618 | - | 1 | 0 | Y | n12-22c27/28o | Unknown | 2 | 17 | ||||||
| YPHF_ECOLI | P77269 | yphF | b2548 JW2532 | periplasmic protein (SEC through IM), ABC transporter periplasmic-binding YphF | Periplasmic | Disulfide bond-containing protein PMID: 25664544 | PMID: 25664544 PMID: 9298646 | Periplasmic | G | N/A | Periplasm | Periplasmic (Experimental) | SEC | no | yes | Y | 27 | 0.817 | SpI | 26 | 20.8859 | - | 0 | 0 | Y | n11-22c26/27o | Periplasmic | 9 | 63 | ||||||||
| RSXG_ECOLI | P77285 | rsxG rnfG ydgP | rnfG ydgP | b1631 JW1623 | periplasmic protein (SEC through IM), Ion-translocating oxidoreductase complex subunit G RsxG | Periplasmic | Disulfide bond-containing protein PMID: 25664544 | PMID: 25664544 | Periplasmic | G | N/A | Cell inner membrane | Periplasmic | SEC | yes | yes | Y | 25 | 0.427 | SpI | 24 | 11.9411 | - | 0 | 0 | Y | n9-17c24/25o | Unknown | 2 | 42 | |||||||
| YDEU_ECOLI | P77286 | ydeU | b1509 JW1502 | OM b-barrel protein (SEC through IM, T5S secreted through OM), YdeU | Outer Membrane b-barrel protein | Ag43 homologue; predicted autotransporter. | PMID: 15590781 | Outer Membrane b-barrel protein | H | N/A | Cytoplasmic | SEC | Autotransporter(T5S) | pseudogene (Uniprot) | no | yes | N | 33 | 0.107 | CYT | 0 | -0.200913 | - | 0 | 0 | N | i | OuterMembrane | 9 | 3 | |||||||
| YFCV_ECOLI | P77288 | yfcV | b2339 JW2336 | Fimbrial periplasmic protein (SEC through IM), YfcV | Periplasmic | Putative chaperone???usher fimbrial operon yfc (class ??); Fimbrial Subunit. | PMID: 20345943 | Periplasmic | G | N/A | Periplasmic | fimbriae | SEC | CU | yes | yes | Y | 26 | 0.533 | SpI | 25 | 14.9857 | - | 1 | 0 | Y | n7-18c25/26o | Unknown | 2 | 97 | |||||||
| YDER_ECOLI | P77294 | ydeR | b1503 JW1497 | Fimbrial periplasmic protein (SEC through IM), YdeR | Periplasmic | Potential fimbrial yde operon (class ??1); Fimbrial Subunit. Disulfide bridge (39 77) | PMID: 19820722 PMID: 20345943 | Periplasmic | G | N/A | Fimbrium | Periplasmic | fimbriae | SEC | CU | no | yes | Y | 24 | 0.701 | SpI | 23 | 20.0135 | - | 0 | 0 | Y | n8-18c23/24o | Unknown | 2 | 67 | ||||||
| YBET_ECOLI | P77296 | ybeT | b0647 JW0642 | OM lipoprotein (SEC through IM), inserted in OM by LOL), Sel1-repeat-containing YbeT | Outer Membrane Lipoprotein | I | N/A | Outer membrane Lipoprotein | SEC | LOL | no | yes | N | 22 | 0.112 | SpII | 18 | 5.89834 | Pos+2=I | 1 | 0 | Y | n8-17c21/22o | Unknown | 2 | 49 | |||||||||||
| YDEN_ECOLI | P77318 | ydeN | b1498 JW5243 | periplasmic protein (SEC through IM), sulfatase YdeN | Periplasmic | G | N/A | Periplasmic | SEC | no | yes | Y | 30 | 0.809 | SpI | 29 | 24.3093 | - | 0 | 0 | Y | n9-20c29/30o | Unknown | 6 | 56 | ||||||||||||
| YAGS_ECOLI | P77324 | yagS | b0285 JW0279 | periplasmic protein (TAT via piggyback through IM), Aldehyde oxidoreductase FAD-binding subunit YagS | Periplasmic | TAT (piggyback) The YagT protein contains a 49 amino acid Tat leader peptide that allows the export of the active heterotrimer to the periplasm (data not shown). Tat substrates are matured in the cytoplasm prior to their translocation. The YagT protein contains a 49 amino acid Tat leader peptide that allows the export of the active heterotrimer to the periplasm (data not shown). PMID: 19368556 | PMID: 17218314 PMID: 19368556 | Periplasmic | G | N/A | Periplasm | Membrane anchored | TAT (piggyback) | no | yes | N | 16 | 0.129 | CYT | 0 | -0.200913 | - | 0 | 0 | N | i | Cytoplasmic | 9 | 15 | ||||||||
| BORD_ECOLI | P77330 | borD ybcU | ybcU | b0557 JW0546 | OM lipoprotein (SEC through IM), inserted in OM by LOL), Prophage Bor homolog BorD | Outer Membrane Lipoprotein | Proven pignal peptidase II substrate. PMID: 15174130 Disulfide bond-containing protein PMID: 25664544 | PMID: 15174130 PMID: 25664544 | Outer Membrane Lipoprotein | I | Soluble IM Fraction | Cell membrane | Outer membrane Lipoprotein (Experimental) | SEC | LOL | Prophage Integrase (EcoGene) | no | no | Y | 21 | 0.303 | SpII | 16 | 21.3732 | Pos+2=A | 0 | 0 | Y | n5-14c18/19o | Unknown | 2 | 32 | |||||
| HLYE_ECOLI | P77335 | hlyE clyA hpr sheA ycgD | clyA hpr sheA ycgD | b1182 JW5181 | periplasmic & extra-cellular protein (SEC through IM), secreted in OMV), Hemolysin E, chromosomal HlyE | Periplasmic, Extra-cellular | HlyE(ClyA) forms a dodecameric transmembrane pore. The ability of ClyA to translocate to the periplasm is abolished in deletion mutants lacking the last 23 or 11 amino acid residues of the C-terminal region. Also, analysis of ClyA mutants with defined deletions and amino acid exchanges revealed that several ClyA segments which are distant of each other in the primary structure, namely, the N-terminal ??A1 region, the C-terminal region, and the hydrophobic sequence ranging from residues 89 to 101, are crucial for ClyA translocation from the E. coli cytoplasm to the periplasm. In the soluble ClyA monomer, these regions are found closely adjacent to each other in the tail domain. ClyA assembles into an oligomeric structure in solution in the absence of either bilayer membranes or detergents at physiological temperature.These oligomers can rearrange to create transmembrane pores when in contact with detergents or biological membranes. ClyA does not form transmembrane pores on E. coli lipid membranes. Recombinant E. coli overexpressing the cloned clyA gene accumulated this haemolysin in the periplasm and released only very small amounts of it into the external medium. PMID: 11902713 Disulfide bridge (87 285) PMID: 14532000 | PMID: 10027972 PMID: 11902713 PMID: 14532000 PMID: 19421192 PMID: 20511497 PMID: 24019520 PMCID: PMC2916382 | Periplasmic, Extra-cellular | G, X | N/A | Secreted Periplasm Host cell membrane | Secreted and released (Experimental) | SEC | OMV | no | yes | N | 58 | 0.117 | CYT | 0 | -0.200913 | - | 1 | 0 | N | i | Extracellular | 10 | 62 | ||||||
| YAFT_ECOLI | P77339 | yafT | b0217 JW0206 | OM lipoprotein (SEC through IM, inserted in OM by LOL), YafT | Outer Membrane Lipoprotein | Proven pignal peptidase II substrate. PMID: 15174130 | PMID: 15174130 | Outer Membrane Lipoprotein | I | N/A | Cell membrane | Outer membrane Lipoprotein (Experimental) | SEC | LOL | no | yes | Y | 21 | 0.197 | SpII | 18 | 23.0916 | Pos+2=A | 0 | 0 | Y | n6-16c21/22o | Unknown | 2 | 61 | |||||||
| MPPA_ECOLI | P77348 | mppA ynaH | ynaH | b1329 JW1322 | periplasmic protein (SEC through IM), murein peptide-binding protein MppA | Periplasmic | PMID: 9495761 | Periplasmic | G | N/A | Periplasm | Periplasmic (Experimental) | SEC | yes | yes | Y | 23 | 0.7 | SpI | 22 | 8.93006 | - | 0 | 0 | Y | n4-14c22/23o | Periplasmic | 10 | 32 | ||||||||
| YAFY_ECOLI | P77365 | yafY | b0251 JW0240 | IM lipoprotein (SEC through IM, inserted in OM by LOL), YafY | Inner Membrane Lipoprotein | PMID: 23138451 | Inner Membrane Lipoprotein | E | N/A | Cell inner membrane | Cytoplasmic | SEC | LOL | Prophage Integrase (EcoGene) | no | no | Y | 21 | 0.302 | SpII | 20 | 17.0831 | Pos+2=D | 0 | 0 | Y | n6-17c21/22o | Unknown | 2 | 27 | |||||||
| YBCL_ECOLI | P77368 | ybcL | b0545 JW0533 | periplasmic protein (SEC through IM), UPF0098 YbcL | Periplasmic | Disulfide bridge (46 129) Disulfide bond-containing protein PMID: 25664544 | PMID: 11439028 PMID: 25664544 | Periplasmic | G | N/A | Periplasm | Periplasmic (Experimental) | SEC | Prophage Integrase (EcoGene) | yes | no | Y | 20 | 0.717 | SpI | 19 | 19.8157 | - | 0 | 0 | Y | n4-15c19/20o | Periplasmic | 10 | 48 | |||||||
| YNFE_ECOLI | P77374 | ynfE | b1587 JW1579 | periplasmic peripheral IM protein (TAT through IM), Putative dimethyl sulfoxide reductase chain YnfE | Peripheral inner membrane protein facing the periplasm | TAT secretion system. The ynfEFGHI operon is a paralogue of the Escherichia coli dmsABC operon. ynfE and ynfF are paralogues of dmsA. ynfG and ynfH are paralogues of dmsB and dmsC, respectively. PMID: 14522592 | PMID: 14522592 PMID: 17218314 | Peripheral inner membrane protein facing the periplasm | F2 | N/A | Cell membrane | Periplasmic | TAT | yes | yes | N | 28 | 0.177 | SpI | 42 | 4.96127 | - | 0 | 0 | Y | n19-37c42/43o | CytoplasmicMembrane | 9 | 21 | ||||||||
| YDHX_ECOLI | P77375 | ydhX | b1671 JW5271 | periplasmic protein (TAT-SEC through IM), Ferredoxin-like YdhX | Periplasmic | TAT secretion system. | PMID: 17218314 | Periplasmic | G | N/A | Periplasmic | TAT,SEC | yes | yes | N | 28 | 0.293 | SpI | 27 | 6.80968 | - | 1 | 0 | Y | n8-19c27/28o | Unknown | 4 | ||||||||||
| SFMD_ECOLI | P77468 | sfmD | b0532 JW0521 | Fimbrial OM b-barrel protein (SEC through IM, inserted in OM by BAM), SfmD | Outer Membrane b-barrel protein | Potential fimbrial sfm operon (class ??1); Usher. Disulfide bridge (840 862) | PMID: 20345943 | Outer Membrane b-barrel protein | H | N/A | Cell outer membrane | Outer Membrane ??-barrel protein | fimbriae | SEC | BAM | yes | yes | N | 36 | 0.404 | TMH | 0 | 3.9822 | - | 1 | 1 | N | i12-32o | OuterMembrane | 10 | |||||||
| YAGR_ECOLI | P77489 | yagR | b0284 JW0278 | periplasmic protein (TAT via piggyback through IM), Aldehyde oxidoreductase molybdenum-binding subunit YagR | Periplasmic | TAT (piggyback) The YagT protein contains a 49 amino acid Tat leader peptide that allows the export of the active heterotrimer to the periplasm. Tat substrates are matured in the cytoplasm prior to their translocation. | PMID: 19368556 | Periplasmic | G | N/A | Periplasm | Periplasmic | TAT (piggyback) | no | yes | N | 60 | 0.121 | CYT | 0 | -0.200913 | - | 0 | 0 | N | i | CytoplasmicMembrane | 9 | 27 | ||||||||
| YDDL_ECOLI | P77519 | yddL | b1472 JW1468 | OM b-barrel protein (SEC through IM), YddL | Outer Membrane b-barrel protein | H | N/A | Periplasmic | SEC | pseudogene (Ochman & Davalos, 2006), pseudogene (Uniprot) | yes | yes | Y | 22 | 0.898 | SpI | 21 | 18.2436 | - | 0 | 0 | Y | n5-16c21/22o | OuterMembrane | 9 | 48 | |||||||||||
| YAHJ_ECOLI | P77554 | yahJ | b0324 JW0316 | periplasmic protein (TAT-SEC through IM), YahJ | Periplasmic | TAT secretion system. | PMID: 17218314 | Periplasmic | G | N/A | Periplasmic | TAT,SEC | no | yes | Y | 34 | 0.674 | SpI | 33 | 11.0447 | - | 0 | 0 | Y | n18-28c33/34o | Unknown | 4 | 12 | |||||||||
| YAIW_ECOLI | P77562 | yaiW | b0378 JW0369 | OM lipoprotein (SEC through IM, inserted in OM by LOL), YaiW | Outer Membrane Lipoprotein | Disulfide bond-containing protein PMID: 25664544 | PMID: 20932056 PMID: 25664544 | Outer Membrane Lipoprotein | I | N/A | Outer membrane Lipoprotein | SEC | LOL | no | yes | Y | 26 | 0.354 | SpII | 20 | 28.9818 | Pos+2=S | 0 | 0 | Y | n7-18c23/24o | Unknown | 2 | 10 | ||||||||
| YDEQ_ECOLI | P77588 | ydeQ | b1502 JW1496 | Fimbrial periplasmic protein (SEC through IM), YdeQ | Periplasmic | Potential fimbrial yde operon (class ??1); Potential tip-adhesin. | PMID: 18498364 PMID: 19820722 PMID: 20345943 | Periplasmic | G | N/A | Fimbrium | Periplasmic | fimbriae | SEC | SURA | CU | no | yes | N | 25 | 0.739 | SpI | 24 | 10.2575 | - | 0 | 0 | Y | n9-19c24/25o | Unknown | 2 | 61 | |||||
| YFCS_ECOLI | P77599 | yfcS | b2336 JW2333 | Fimbrial periplasmic protein (SEC through IM), Probable chaperone YfcS | Periplasmic | Putative chaperone???usher fimbrial operon yfc (class ??); Chaperone. Potential substrate of periplasmic peptidyl prolyl cis???trans isomerase SurA; sequence contains known binding motif of SurA [RYEF]X[RYEF][P]. | PMID: 18498364 | Periplasmic | G | N/A | Periplasm | Periplasmic | fimbriae | SEC | SURA | yes | yes | Y | 29 | 0.747 | SpI | 28 | 11.2467 | - | 0 | 0 | Y | n10-21c28/29o | Periplasmic | 10 | 77 | ||||||
| YQIH_ECOLI | P77616 | yqiH | b3047 JW5508 | Fimbrial OM b-barrel protein (SEC through IM, inserted in OM by BAM), Chaperone YqiH | Outer Membrane b-barrel protein | Putative chaperone???usher fimbrial operon (class ??); Chaperone. | PMID: 20345943 | Outer Membrane b-barrel protein | H | N/A | Periplasm | Outer Membrane ??-barrel protein | fimbriae | SEC | BAM | no | yes | Y | 26 | 0.426 | SpI | 25 | 12.7072 | - | 1 | 0 | Y | n9-20c25/26o | Periplasmic | 10 | 21 | ||||||
| YFEW_ECOLI | P77619 | yfeW | b2430 JW5395 | periplasmic protein (SEC through IM), UPF0214 YfeW | Periplasmic | G | N/A | Periplasmic | SEC | no | yes | Y | 20 | 0.512 | SpI | 19 | 10.3062 | - | 0 | 0 | Y | n6-14c19/20o | Periplasmic | 9 | |||||||||||||
| ECPD_ECOLI | P77694 | ecpD matE yagW | matE yagW | b0290 JW0284 | Fimbrial OM b-barrel protein (SEC through IM, inserted in OM by BAM), Fimbria adhesin EcpD EcpD | Outer Membrane b-barrel protein | Putative chaperone???usher fimbrial operon yag(class ??); Unknown function. Putative Outer membrane protein (BOMB prediction + SignalP 98% prediction). | PMID: 20345943 | Outer Membrane b-barrel protein | H | N/A | Fimbrium | Cytoplasmic | fimbriae | SEC | BAM | no | yes | N | 32 | 0.295 | SpI | 23 | 7.3263 | - | 0 | 0 | Y | n5-16c23/24o | Unknown | 4 | 2 | |||||
| YBAY_ECOLI | P77717 | ybaY | b0453 JW0443 | OM lipoprotein (SEC through IM, inserted in OM by LOL), YbaY | Outer Membrane Lipoprotein | Proven pignal peptidase II substrate. PMID: 15174130 | PMID: 15174130 | Outer Membrane Lipoprotein | I | N/A | Cell membrane | Outer membrane Lipoprotein (Experimental) | SEC | LOL | yes | yes | Y | 18 | 0.379 | SpII | 18 | 21.022 | Pos+2=A | 0 | 0 | Y | n3-14c19/20o | Periplasmic | 9 | ||||||||
| OMPN_ECOLI | P77747 | ompN ynaG | ynaG | b1377 JW1371 | OM b-barrel protein (SEC through IM, inserted in OM by BAM), OmpN | Outer Membrane b-barrel protein | PMID: 20932056 PMID: 9642192 | Outer Membrane b-barrel protein | H | N/A | Cell outer membrane; Multi-pass membrane protein | Outer Membrane ??-barrel protein (Experimental) | SEC | BAM | yes | yes | Y | 22 | 0.895 | SpI | 21 | 24.6239 | - | 0 | 0 | Y | n5-16c21/22o | OuterMembrane | 10 | 10 | |||||||
| SPY_ECOLI | P77754 | spy | b1743 JW1732 | periplasmic protein (SEC through IM), chaperone Spy | Periplasmic | PMID: 9068658 | Periplasmic | G | N/A | Periplasm | Periplasmic (Experimental) | SEC | yes | yes | Y | 24 | 0.817 | SpI | 23 | 20.7622 | - | 0 | 0 | Y | n4-15c23/24o | Periplasmic | 10 | 104 | |||||||||
| BAMB_ECOLI | P77774 | bamB yfgL | yfgL | b2512 JW2496 | OM lipoprotein (SEC through IM, inserted in OM by LOL), assembly factor BamB | Outer Membrane Lipoprotein | Part of bam complex. Interacts directry with BamA. Proven pignal peptidase II substrate. Outer membrane protein determined by 2D gel electrophoresis (Lopez-Campistrous et al 2005) PMID: 15174130 | PMID: 15174130 PMID: 15851030 PMID: 15911532 PMID: 20932056 PMID: 21236263 PMID: 21370981 PMID: 21823654 | Outer Membrane Lipoprotein | I | Soluble IM Fraction | Cell outer membrane | Outer membrane Lipoprotein (Experimental) | SEC | LOL | yes | yes | Y | 27 | 0.152 | SpII | 19 | 14.7873 | Pos+2=S | 0 | 0 | Y | n6-17c25/26o | OuterMembrane | 10 | |||||||
| YNFF_ECOLI | P77783 | ynfF | b1588 JW5260 | periplasmic peripheral IM protein (TAT-SEC through IM), Probable dimethyl sulfoxide reductase chain YnfF | Peripheral inner membrane protein facing the periplasm | Verified Tat substrate: YnfF has a Tat-only (Class II) predicted 45 aa signal peptide (Tullman-Ercek, 2007). The physiological substrate and relevance of the YnfFGH S- and N-oxide reductase is unknown.; DmsA paralog.So far, hydroxy pyridine N-oxide is the The ynfEFGHI operon is a paralogue of the Escherichia coli dmsABC operon. ynfE and ynfF are paralogues of dmsA. ynfG and ynfH are paralogues of dmsB and dmsC, respectively. PMID: 14522592 | PMID: 14522592 PMID: 17218314 PMID: 19415239 | Peripheral inner membrane protein facing the periplasm | F2 | N/A | Cell membrane | Periplasmic | TAT,SEC | yes | yes | N | 46 | 0.406 | SpI | 45 | 11.1244 | - | 0 | 0 | Y | n24-37c45/46o | CytoplasmicMembrane | 9 | 9 | ||||||||
| YDES_ECOLI | P77789 | ydeS | b1504 JW1498 | Fimbrial extra-cellular peripheral OM protein (SEC through IM, chaperone usher export system over OM), YdeS | Peripheral outer membrane protein facing the extra-cellular space | Potential fimbrial yde operon (class ??1); Fimbrial Subunit. Disulfide bridge (38 78) | PMID: 19820722 PMID: 20345943 | Peripheral outer membrane protein facing the extra-cellular space | F4 | N/A | Fimbrium | Membrane anchored | fimbriae | SEC | CU | no | yes | Y | 23 | 0.426 | SpI | 22 | 11.5353 | - | 1 | 0 | Y | n6-17c22/23o | Extracellular | 10 | 79 | ||||||
| ECPC_ECOLI | P77802 | ecpC matD yagX | matD yagX | b0291 JW0285 | Fimbrial periplasmic protein (SEC through IM), Probable usher EcpC | Periplasmic | Putative chaperone???usher fimbrial operon yag(class ??); Usher. Potential substrate of periplasmic peptidyl prolyl cis???trans isomerase SurA; sequence contains known binding motif of SurA [RYEF]X[RYEF][P]. | PMID: 18498364 PMID: 20345943 | Periplasmic | G | N/A | Periplasmic | fimbriae | SEC | SURA | no | yes | Y | 30 | 0.522 | SpI | 29 | 1.61886 | - | 0 | 0 | Y | n12-23c29/30o | Unknown | 4 | |||||||
| YDGA_ECOLI | P77804 | ydgA | b1614 JW1606 | periplasmic peripheral IM protein (SEC through IM), YdgA | Peripheral inner membrane protein facing the periplasm | Identified in nucleoids collected by sucrose gradient centrifugation and analyzed by liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS). PMID: 21541338 | PMID: 16079137 PMID: 21541338 | Peripheral inner membrane protein facing the periplasm | F2 | Soluble IM Fraction | Cell inner membrane | Cytoplasmic | SEC | yes | yes | N | 25 | 0.174 | SpI | 24 | 3.81151 | - | 1 | 0 | Y | n4-14c19/20o | CytoplasmicMembrane | 10 | 25 | ||||||||
| YNJE_ECOLI | P78067 | ynjE | b1757 JW5287 | periplasmic protein (SEC through IM), Thiosulfate sulfurtransferase YnjE | Periplasmic | Potential substrate of periplasmic peptidyl prolyl cis???trans isomerase SurA; sequence contains known binding motif of SurA [RYEF]X[RYEF][P]. Potential substrate of periplasmic chaperone YfgM. PMID: 25403562 Putative substrate for the periplasmic chaperone YfgM. PMID: 25403562 | PMID: 18498364 PMID: 25403562 | Periplasmic | G | N/A | Periplasm | Periplasmic | SEC | SURA,YfgM | yes | yes | Y | 24 | 0.698 | SpI | 23 | 21.4185 | - | 0 | 0 | Y | n8-18c23/24o | Periplasmic | 10 | 40 | |||||||
| YSAB_ECOLI | Q2M7M3 | ysaB | b4553 JW3532 | OM lipoprotein (SEC through IM, inserted in OM by LOL), YsaB | Outer Membrane Lipoprotein | I | N/A | Cell membrane | Outer membrane Lipoprotein | SEC | LOL | yes | yes | Y | 27 | 0.418 | SpII | 17 | 15.6662 | Pos+2=S | 0 | 0 | Y | n5-16c26/27o | Unknown | 2 | 36 | ||||||||||
| YICS_ECOLI | Q2M7X4 | yicS | b4555 JW5965 | periplasmic protein (SEC through IM), YicS | Periplasmic | Disulfide bond-containing protein PMID: 25664544 | PMID: 25664544 | Periplasmic | G | N/A | Periplasmic | SEC | yes | yes | N | 22 | 0.681 | SpI | 21 | 10.9898 | - | 0 | 0 | Y | n4-14c21/22o | Unknown | 2 | ||||||||||
| PBL_ECOLI | Q46790 | pbl | b2854 | periplasmic protein (SEC through IM), Putative PG-binding-like protein Pbl | Periplasmic | G | N/A | Periplasm | Cytoplasmic | SEC | pseudogene (Uniprot) | no | no | Y | 25 | 0.548 | SpI | 24 | 2.39101 | - | 0 | 0 | Y | n8-19c24/25o | Periplasmic | 9 | 28 | ||||||||||
| YGEQ_ECOLI | Q46797 | ygeQ | b2863 JW5461 | periplasmic protein (SEC through IM), YgeQ | Periplasmic | G | N/A | Periplasmic | SEC | pseudogene (Uniprot), pseudogene (Ochman & Davalos, 2006) | no | no | Y | 37 | 0.449 | SpI | 36 | 11.1359 | - | 0 | 0 | Y | n20-31c36/37o | Unknown | 2 | 68 | |||||||||||
| YGER_ECOLI | Q46798 | ygeR | b2865 JW2833 | OM lipoprotein (SEC through IM, inserted in OM by LOL), YgeR | Outer Membrane Lipoprotein | Proven pignal peptidase II substrate. PMID: 15174130 | PMID: 15174130 PMID: 20932056 | Outer Membrane Lipoprotein | I | N/A | Cell inner membrane | Outer membrane Lipoprotein (Experimental) | SEC | LOL | yes | yes | N | 33 | 0.262 | SpII | 25 | 22.1379 | Pos+2=S | 0 | 0 | Y | n11-23c28/29o | OuterMembrane | 9 | 58 | |||||||
| YGHF_ECOLI | Q46834 | yghF | b2970 JW5484 | periplasmic protein (SEC through IM), Putative type II secretion system C-type YghF | Periplasmic | G | N/A | Periplasm | Cytoplasmic | SEC | pseudogene (Uniprot) | no | yes | N | 43 | 0.145 | SpI | 16 | 1.1016 | - | 0 | 0 | Y | n2-9c14/15o | CytoplasmicMembrane | 9 | 36 | ||||||||||
| YGHG_ECOLI | Q46835 | yghG | b2971 JW2938 | OM lipoprotein (SEC through IM, inserted in OM by LOL), YghG | Outer Membrane Lipoprotein | Belongs to the GSP C family. Proven pignal peptidase II substrate. PMID: 15174130 | PMID: 15174130 | Outer Membrane Lipoprotein | I | N/A | Cell membrane | Outer membrane Lipoprotein (Experimental) | SEC | LOL | no | yes | Y | 29 | 0.253 | SpII | 24 | 17.6171 | Pos+2=A | 0 | 0 | Y | n10-22c27/28o | Unknown | 2 | 40 | |||||||
| YQHG_ECOLI | Q46858 | yqhG yqhF | yqhF | b3013 JW5500 | periplasmic protein (SEC through IM), YqhG | Periplasmic | Potential substrate of periplasmic peptidyl prolyl cis???trans isomerase SurA; sequence contains known binding motif of SurA [RYEF]X[RYEF][P]. | PMID: 18498364 | Periplasmic | G | N/A | Periplasmic | SEC | SURA | no | yes | Y | 19 | 0.889 | SpI | 18 | 17.8226 | - | 0 | 0 | Y | n3-14c18/19o | Unknown | 4 | 84 | |||||||
| YGIS_ECOLI | Q46863 | ygiS | b3020 JW2988 | periplasmic protein (SEC through IM), Probable deoxycholate-binding periplasmic YgiS | Periplasmic | G | N/A | Periplasm | Periplasmic | SEC | yes | yes | Y | 21 | 0.755 | SpI | 20 | 13.5931 | - | 0 | 0 | Y | n6-16c20/21o | Periplasmic | 9 | 27 | |||||||||||
| YAFL_ECOLI | Q47151 | yafL | b0227 JW0217 | periplasmic protein (SEC through IM), Probable endopeptidase YafL | Periplasmic | G | N/A | Cell membrane | Periplasmic | SEC | yes | yes | Y | 26 | 0.582 | SpI | 27 | 11.7588 | - | 1 | 0 | Y | n8-19c37/38o | Unknown | 4 | 34 | |||||||||||
| YAIO_ECOLI | Q47534 | yaiO | b0358 JW0349 | periplasmic protein (SEC through IM), YaiO | <a href="http://beta-barrel.tulane.edu/">TMBB-DB</a> tool predicts this protein as an outer membrane beta barrel protein. | Periplasmic | G | N/A | Cell outer membrane | Periplasmic | SEC | no | yes | Y | 20 | 0.841 | SpI | 19 | 12.701 | - | 1 | 0 | Y | n5-15c19/20o | Unknown | 2 | 5 | ||||||||||
| TAUA_ECOLI | Q47537 | tauA ssiA yaiR | ssiA yaiR | b0365 JW0357 | periplasmic protein (SEC through IM), Taurine-binding periplasmic TauA | Periplasmic | PMID: 8774726 | Periplasmic | G | N/A | Periplasm | Periplasmic (Experimental) | SEC | no | yes | Y | 23 | 0.816 | SpI | 22 | 16.0488 | - | 0 | 0 | Y | n8-18c22/23o | Periplasmic | 9 | |||||||||
| SAPA_ECOLI | Q47622 | sapA | b1294 JW1287 | periplasmic protein (SEC through IM), Probable ABC transporter periplasmic-binding SapA | Periplasmic | Potential substrate of periplasmic peptidyl prolyl cis???trans isomerase SurA; sequence contains known binding motif of SurA [RYEF]X[RYEF][P]. PMID: 9593292 | PMID: 18498364 PMID: 9593292 | Periplasmic | G | N/A | Periplasm | Periplasmic (Experimental) | SEC | SURA | yes | yes | Y | 22 | 0.865 | SpI | 21 | 14.8036 | - | 0 | 0 | Y | n4-15c21/22o | Periplasmic | 10 | 30 | |||||||
| YFEK_ECOLI | Q47702 | yfeK | b2419 JW2412 | periplasmic protein (SEC through IM), YfeK | Periplasmic | G | N/A | Periplasmic | SEC | no | yes | Y | 20 | 0.758 | SpI | 19 | 2.85333 | - | 0 | 0 | Y | n4-15c19/20o | Unknown | 2 | 77 | ||||||||||||
| UIDC_ECOLI | Q47706 | uidC gusC | gusC | b1615 JW1607 | OM b-barrel protein (SEC through IM, inserted in OM by BAM), Membrane-associated UidC UidC | Outer Membrane b-barrel protein | PMID: 15774881 | Outer Membrane b-barrel protein | H | N/A | Cell outer membrane | Outer Membrane ??-barrel protein (Experimental) | SEC | BAM | no | yes | Y | 24 | 0.665 | SpI | 23 | 13.3065 | - | 1 | 0 | Y | n4-15c23/24o | OuterMembrane | 9 | 3 | |||||||
| YJDP_ECOLI | Q6BEX5 | yjdP | b4487 JW5890 | periplasmic protein (SEC through IM), YjdP | Periplasmic | G | N/A | Periplasmic | SEC | no | yes | Y | 23 | 0.721 | SpI | 22 | 14.9551 | - | 0 | 0 | Y | n6-17c22/23o | Unknown | 2 |