entry

acc

gene

alt_gene

oln

protein_name

cm_loca

cm_notes

cm_refs

suboci_full

subloci

bernsel

subloci_uniprot

subloci_echoL

organelle

target_chaperoning1

1st_ss

target_chaperoning2

2nd_ss

pseudo

common_core

basic_proteome

signalp_sp_exist

signalp_pos

signalp_cmax

lipop_pred

lipop_cleavage

lipop_score

lipop_rule

tmhmm

phobius_tms

phobius_sp_exist

phobius_prediction

psortb_loca

psortb_score

solubility

nterm_cterm

SGRT_ECOLI

C1P5Z7

sgrT

b4662 JW0068.1

cytoplasmic peripheral IM protein, Putative inhibitor of glucose uptake transporter SgrT

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)

Putative inhibitor of glucose uptake transporter sgrT. SgrT protein expresssion is inducted by ??-methylglucoside.

[18042713]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

-

no

yes

N

23

0.105

CYT

0

-0.200913

-

0

0

N

i

Unknown

2

YOBI_ECOLI

C1P604

yobI

b4677 JW1850.1

cytoplasmic peripheral IM protein, YobI

Small membrane protein detected experimentally

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

-

no

yes

N

12

0.1

CYT

0

-0.200913

-

0

0

N

i

Unknown

2

AZUC_ECOLI

C1P605

azuC

b4663 JW1891.1

cytoplasmic peripheral IM protein, AzuC

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)

Small protein detected experimentally. AzuC-SPA was most abundant in the inner membrane fraction (77%) indicating this protein is bound to the cytoplasmic side of the inner membrane.

[19121005] [21778229]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cell inner membrane

-

no

yes

N

27

0.099

CYT

0

-0.200913

-

0

0

N

i

Unknown

2

YSHB_ECOLI

C1P620

yshB

b4686 JW3839.1

cytoplasmic peripheral IM protein, YshB

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Potential)

Amphipaseek predicts N-terminal amphipathic helix (1-10). Amphipaseek predicts N-terminal amphipathic helix (1-10).

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

-

no

yes

N

25

0.194

CYT

0

-0.200913

-

0

0

N

i

Unknown

2

6PGD_ECOLI

P00350

gnd

b2029 JW2011

cytoplasmic peripheral IM protein, 6-phosphogluconate dehydrogenase, decarboxylating Gnd

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

[23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasmic

yes

yes

N

20

0.182

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

8

90

FRDA_ECOLI

P00363

frdA

b4154 JW4115

cytoplasmic peripheral IM protein, Fumarate reductase flavosubunit FrdA

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)

FrdA and FrdB Fumarate reductase The terminal electron-transfer enzyme fumarate reductase of Escherichia coli is a complex iron-sulfur flavoenzyme composed of four nonidentical subunits organized into two domains: FrdA and -B (a membrane-extrinsic catalytic domain) and FrdC and -D (a transmembrane anchor domain). [PMC323229] Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279]

[15919657] [23230279] [PMC323229]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

Soluble IM Fraction

Cytoplasmic

yes

yes

N

28

0.271

SpI

27

1.50227

-

0

0

Y

n8-18c27/28o

CytoplasmicMembrane

9

50

DHNA_ECOLI

P00393

ndh

b1109 JW1095

cytoplasmic peripheral IM protein, NADH dehydrogenase Ndh

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

Contains amphipathic C-terminal anchor (390-406). Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279]

[20933494] [23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

IM (predicted)

Cell membrane

Cytoplasmic

yes

yes

N

22

0.114

CYT

0

-0.200913

-

0

0

N

i

CytoplasmicMembrane

10

35

RIR1_ECOLI

P00452

nrdA

dnaF

b2234 JW2228

cytoplasmic peripheral IM protein, Ribonucleoside-diphosphate reductase 1 subunit alpha NrdA

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

Trigger factor-sensitive aggregation. Disulfide bridges between 225 and 462 [9395490] Disulfide bridges between 225 and 462 [9395490] Disulfide bridges between 225 and 462 [9395490]

[23230279] [9395490]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasmic

TF

yes

yes

N

40

0.104

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

9

63

AAT_ECOLI

P00509

aspC

b0928 JW0911

cytoplasmic peripheral IM protein, Aspartate aminotransferase AspC

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

[23230279] [3521591]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasm

Cytoplasmic (Experimental)

yes

yes

N

23

0.126

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

10

AK1H_ECOLI

P00561

thrA

thrA1 thrA2

b0002 JW0001

cytoplasmic peripheral IM protein, Bifunctional aspartokinase/homoserine dehydrogenase 1 ThrA

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)

Copurifies with trigger factor in pull down assays. Identified in the inner membrane by biochemical fractionation followed by two-dimensional gel electrophoresis and tandem mass spectrometry. [15911532] [4562989] [354697] [7003595] [6777249]

[15911532] [354697] [4562989] [6777249] [7003595]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasmic (Experimental)

TF

yes

yes

N

15

0.142

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

8

AK2H_ECOLI

P00562

metL

metM

b3940 JW3911

cytoplasmic peripheral IM protein, Bifunctional aspartokinase/homoserine dehydrogenase 2 MetL

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

Disulfide bond-containing protein [25664544]

[23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasmic

yes

yes

N

24

0.117

CYT

0

-0.200913

-

0

0

N

i

CytoplasmicMembrane

9

RPOD_ECOLI

P00579

rpoD

alt

b3067 JW3039

cytoplasmic nucleoid peripheral IM protein, RNA polymerase sigma factor RpoD

Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (Experimental)

Trigger factor-sensitive aggregation.

[23230279]

Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm

A, N, F1

N/A

Cytoplasm

Cytoplasmic

TF

yes

yes

N

26

0.104

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

9

92

DPO1_ECOLI

P00582

polA

resA

b3863 JW3835

cytoplasmic nucleoid peripheral IM protein, DNA polymerase I PolA

Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (Experimental)

[23230279]

Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm

A, N, F1

N/A

Cytoplasmic

no

yes

N

59

0.268

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

9

76

CAPP_ECOLI

P00864

ppc

glu

b3956 JW3928

cytoplasmic peripheral IM protein, Phosphoenolpyruvate carboxylase Ppc

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

[23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasmic

yes

yes

N

48

0.115

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

8

TRPE_ECOLI

P00895

trpE

b1264 JW1256

cytoplasmic peripheral IM protein, Anthranilate synthase component 1 TrpE

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)

Experimentally detected by proteomics analysis of E.coli membrane fraction (Bernsel et al 2009). No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Potential peripherally associated protein facing the cytoplasm or integral inner membrane protein.

[19766000]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

Soluble IM Fraction

Cytoplasmic

yes

yes

N

19

0.11

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

10

66

TRPGD_ECOLI

P00904

trpGD

trpD

b1263 JW1255

cytoplasmic peripheral IM protein, Bifunctional TrpG

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)

Could not obtain any evidence for membrane binding (F1) Peripheral IM Cytoplasmic side. Identified in the inner membrane by biochemical fractionation followed by two-dimensional gel electrophoresis and tandem mass spectrometry. [15911532] [4594441] [6283099]

[15911532] [19766000] [4594441] [6283099]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

Soluble IM Fraction

Cytoplasmic (Experimental)

yes

yes

N

19

0.166

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

9

22

SYA_ECOLI

P00957

alaS

lovB

b2697 JW2667

cytoplasmic nucleoid peripheral IM protein, Alanine--tRNA ligase AlaS

Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (Experimental)

Trigger factor-sensitive aggregation.

[23138451] [23230279]

Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm

A, N, F1

N/A

Cytoplasm

Cytoplasmic

TF

no

yes

N

26

0.129

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

9

57

SYM_ECOLI

P00959

metG

b2114 JW2101

cytoplasmic peripheral IM protein, Methionine--tRNA ligase MetG

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

Translation unit. Trigger factor-sensitive aggregation.

[23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasm

Cytoplasmic

TF

yes

yes

N

20

0.115

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

10

49

SYGA_ECOLI

P00960

glyQ

glyS(A)

b3560 JW3531

cytoplasmic peripheral IM protein, Glycine--tRNA ligase alpha subunit GlyQ

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

Translation unit. Trigger factor-sensitive aggregation. Disulfide bond-containing protein [25664544]

[23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasm

Cytoplasmic

TF

no

yes

N

57

0.112

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

9

59

SYGB_ECOLI

P00961

glyS

glyS(B)

b3559 JW3530

cytoplasmic peripheral IM protein, Glycine--tRNA ligase beta subunit GlyS

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

Translation unit.

[23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasm

Cytoplasmic

yes

yes

N

65

0.169

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

10

77

CARB_ECOLI

P00968

carB

pyrA

b0033 JW0031

cytoplasmic peripheral IM protein, Carbamoyl-phosphate synthase large chain CarB

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

Copurifies with trigger factor in pull down assays.

[23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasmic

TF

yes

yes

N

24

0.228

CYT

0

-0.200913

-

0

0

Y

n9-16c21/22o

Unknown

4

DNAA_ECOLI

P03004

dnaA

b3702 JW3679

cytoplasmic nucleoid peripheral IM protein, Chromosomal replication initiator DnaA

Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (Experimental)

C-terminal amphiphilic helix (355-370). Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279]

[10660626] [23138451] [23230279]

Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm

A, N, F1

N/A

Cytoplasm

Cytoplasmic

no

yes

N

32

0.146

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

10

38

LACI_ECOLI

P03023

lacI

b0345 JW0336

cytoplasmic peripheral IM protein, Lactose operon repressor LacI

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)

Regulates lac operon transcription when in homotetrameric form.

[15863616] [21936772] [23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasmic

no

yes

N

33

0.136

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

10

32

GUAA_ECOLI

P04079

guaA

b2507 JW2491

cytoplasmic peripheral IM protein, GMP synthase GuaA

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

Trigger factor-sensitive aggregation.

[23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasmic

TF

yes

yes

N

45

0.122

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

9

AMPN_ECOLI

P04825

pepN

b0932 JW0915

cytoplasmic peripheral IM protein, Aminopeptidase PepN

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (By similarity) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

Homologous peptidases are membrane associated in mammals, some data propose that the enzyme may associate with the E.coli membrane. It is structurally classified with <a href="http://www.ebi.ac.uk/thornton-srv/databases/cgi-bin/enzymes/GetPage.pl?ec_number=3.4.11.2">EC 3.4.11.2</a> membrane alanyl aminopeptidases. However, the extent of membrane association needs further analysis as it is generally soluble in the cytoplasmic fraction of E.coli and other bacteria. [323010] Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279]

[23230279] [323010]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cell inner membrane; Peripheral membrane protein; Cytoplasmic side

Cytoplasmic (Experimental)

yes

yes

N

50

0.108

CYT

0

-0.200913

-

0

0

N

i

CytoplasmicMembrane

10

101

RBSA_ECOLI

P04983

rbsA

b3749 JW3728

cytoplasmic peripheral IM protein, Ribose import ATP-binding RbsA

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

Soluble IM Fraction

Cell inner membrane

Cytoplasmic

yes

yes

N

35

0.548

CYT

0

-0.200913

-

0

0

N

i

CytoplasmicMembrane

10

49

FUMC_ECOLI

P05042

fumC

b1611 JW1603

cytoplasmic peripheral IM protein, Fumarate hydratase class II FumC

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

[23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasm

Cytoplasmic

yes

yes

N

57

0.12

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

10

64

PNP_ECOLI

P05055

pnp

b3164 JW5851

cytoplasmic peripheral IM protein, Polyribonucleotide nucleotidyltransferase Pnp

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)

Polynucleotide phosphorylase (PNPase) or Polyribonucleotide nucleotidyltransferase Is a cytoplasmic enzyme that has also been isolated in association with the inner membrane (Stenberg et al., 2005; Maddalo et al., 2011). It is not known if association is direct. The protein has weak hydrophobic patches and three regions with weak predictions from Phobius for membrane interaction. Membrane association may be via binding to RNAaseE with which PNPase interacts as part of the multienzyme complex known as the RNA degradosome. RNAseE has been shown to bind to membranes (Khemici et al., 2008). The interaction of RNase E with PNPase , brings together two enzymes important in the degradation of mRNA. Trigger factor-sensitive aggregation. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279] Part of membrane-associating assemblies that coil around the periphery of the cell when visualized by immunofluorescence microscopy. These assemblies co-localize with the RNA degradosome and participate in the RNA degradation process. [24438330]

[16079137] [23230279] [24438330]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

Soluble IM Fraction

Cytoplasm

Cytoplasmic

TF

yes

yes

N

30

0.323

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

10

74

PTHA_ECOLI

P05706

srlB

gutB

b2704 JW2673

cytoplasmic peripheral IM protein, PTS system glucitol/sorbitol-specific EIIA component SrlB

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Potential)

PTS system glucitol/sorbitol-specific EIIA component.The hydropathic profile of the Enzyme IIgut revealed 7 or 8 long hydrophobic segments which may traverse the cell membrane as alpha-helices as well as 2 or 4 short strongly hydrophobic stretches which may traverse the membrane as beta-structure [3553176].

[2824435] [3553176]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasm

Cytoplasmic

no

yes

N

35

0.143

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

8

115

ILVD_ECOLI

P05791

ilvD

b3771 JW5605

cytoplasmic peripheral IM protein, Dihydroxy-acid dehydratase IlvD

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

[23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasmic

no

yes

N

54

0.166

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

9

25

ILVC_ECOLI

P05793

ilvC

b3774 JW3747

cytoplasmic peripheral IM protein, Ketol-acid reductoisomerase IlvC

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

[23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

Soluble IM Fraction

Cytoplasm

Cytoplasmic

yes

yes

N

29

0.107

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

10

97

BGLR_ECOLI

P05804

uidA

gurA gusA

b1617 JW1609

cytoplasmic peripheral IM protein, Beta-glucuronidase UidA

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)

Experimentally detected by proteomics analysis of E.coli membrane fraction (Bernsel et al 2009). No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Potential peripherally associated protein facing the cytoplasm or integral inner membrane protein.

[19766000]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

Soluble IM Fraction

Cytoplasmic

no

yes

N

59

0.12

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

8

46

DLD_ECOLI

P06149

dld

b2133 JW2121

cytoplasmic peripheral IM protein, Quinone-dependent D-lactate dehydrogenase Dld

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

Requires phospholipid for maximal activity. Exhibites aberrant localization by microscopy in the absence of YidC. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279]

[15919657] [23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

Soluble IM Fraction

Cell inner membrane

Cytoplasmic

yes

yes

N

33

0.121

CYT

0

-0.200913

-

0

0

N

i

CytoplasmicMembrane

10

78

BTUD_ECOLI

P06611

btuD

b1709 JW1699

cytoplasmic peripheral IM protein, Vitamin B12 import ATP-binding BtuD

Vitamin B12 import ATP-binding protein btuD.

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cell inner membrane; Peripheral membrane protein

Cytoplasmic

yes

yes

N

26

0.195

CYT

0

-0.200913

-

0

0

N

i

CytoplasmicMembrane

10

9

ERA_ECOLI

P06616

era

rbaA sdgE

b2566 JW2550

ribosomal cytoplasmic peripheral IM protein, GTPase Era

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm & ribosomal (Experimental) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm & ribosomal (Probable)

Assembly cofactor of ribosome; associate with the 30S subunit and 16S rRNA. YeaA has been viewed through fluorescence microscopy and located evenly throughout cytoplasm. Small scale study of labeling chromosomal coding DNA sequences (CDSs) with fluorescent protein genes. [17272300]

[17272300] [8282709]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm, ribosomal

A, F1, r

N/A

Cytoplasm

Cytoplasmic

yes

yes

N

41

0.127

CYT

0

-0.200913

-

0

0

N

i

CytoplasmicMembrane

10

92

ODP2_ECOLI

P06959

aceF

b0115 JW0111

cytoplasmic peripheral IM protein, Dihydroyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex AceF

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

Trigger factor-sensitive aggregation. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279]

[15911532] [15919657] [23230279] [24742683]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

Soluble IM Fraction

Cytoplasmic

TF

no

yes

N

52

0.106

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

10

95

FLIM_ECOLI

P06974

fliM

cheC2 fla AII fla QII

b1945 JW1929

Flagellar cytoplasmic peripheral IM protein, motor switch FliM

Flaggelar C-ring proteins - FliG, FliM, and FliN.

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

IM (predicted)

Cell inner membrane; Peripheral membrane protein Bacterial flagellum basal body

Cytoplasmic

flagellum

no

yes

N

10

0.109

CYT

0

-0.200913

-

0

0

N

i

CytoplasmicMembrane

10

57

HIS7_ECOLI

P06987

hisB

b2022 JW2004

cytoplasmic peripheral IM protein, Histidine biosynthesis bifunctional HisB

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

Copurifies with trigger factor in pull down assays.

[23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasm

Cytoplasmic

TF

yes

yes

N

24

0.105

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

10

66

RSMA_ECOLI

P06992

rsmA

ksgA

b0051 JW0050

cytoplasmic peripheral IM protein, Ribosomal RNA small subunit methyltransferase A RsmA

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

Assembly cofactor of ribosome; Overex<x>pression of RsmA suppresses defects in mutant of era. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279]

[21529161] [23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasm

Cytoplasmic

yes

yes

N

33

0.128

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

10

MALT_ECOLI

P06993

malT

malA

b3418 JW3381

cytoplasmic nucleoid peripheral IM protein, HTH-type transcriptional regulator MalT

Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (Experimental) Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (Potential)

Cytoplasmic transciption factor that binds maltotriose and ATP and shuttles between the chromosome and membranes as a peripheral protein on the MalK peripheral ATPase of the maltose transporter. Trigger factor-sensitive aggregation. LuxR-type HTH domain signature and profile. The luxR-type HTH domain is a DNA-binding, helix-turn-helix (HTH) domain of about 65 amino acids, present in transcription regulators of the LuxR/FixJ family of response regulators (PROSITE: PDOC0054).

[15063852] [23138451]

Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm

A, N, F1

N/A

Cytoplasmic

TF

yes

yes

N

36

0.12

CYT

0

-0.200913

-

0

0

N

i

Unknown

7

8

PLDB_ECOLI

P07000

pldB

b3825 JW5584

cytoplasmic peripheral IM protein, Lysophospholipase L2 PldB

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

Gene and characteristics of deduced amino acid sequence of lysophospholipase L2. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279]

[23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cell inner membrane; Peripheral membrane protein

Cytoplasmic

yes

yes

N

34

0.104

CYT

0

-0.200913

-

0

0

N

i

CytoplasmicMembrane

7

36

POXB_ECOLI

P07003

poxB

b0871 JW0855

cytoplasmic peripheral IM protein, Pyruvate dehydrogenase PoxB

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Potential)

Is one of several membrane-associated form of the enzyme has a tendency to self-aggregate,is flavo protein dehydrogenases that feed electrons in to the high lysurfacelabile,and manifests. Pyruvate oxidase is a membrane-associated flavoprotein dehydrogenase which is greatly activated by lipids and detergents. The carboxyl-terminal region of the protein has been shown to play a critical role in the interaction with lipids.

[2663858]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cell membrane; Peripheral membrane protein

Cytoplasmic

yes

yes

N

62

0.106

CYT

0

-0.200913

-

0

0

N

i

CytoplasmicMembrane

10

20

PROA_ECOLI

P07004

proA

b0243 JW0233

cytoplasmic peripheral IM protein, Gamma-glutamyl phosphate reductase ProA

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

[23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasm

Cytoplasmic

yes

yes

N

48

0.168

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

10

118

SDHB_ECOLI

P07014

sdhB

b0724 JW0714

cytoplasmic peripheral IM protein, Succinate dehydrogenase iron-sulfur subunit SdhB

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)

Identified in nucleoids collected by sucrose gradient centrifugation and analyzed by liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS). [21541338] [6388571] [2987185] Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279]

[16079137] [23230279] [2987185] [6388571]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

IM (predicted)

Cell inner membrane

Cytoplasmic (Experimental)

yes

yes

N

28

0.102

CYT

0

-0.200913

-

0

0

N

i

CytoplasmicMembrane

10

34

HISP_ECOLI

P07109

hisP

b2306 JW2303

cytoplasmic peripheral IM protein, Histidine transport ATP-binding HisP

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279]

[23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

Soluble IM Fraction

Cell inner membrane; Peripheral membrane protein

Cytoplasmic

yes

yes

N

31

0.431

CYT

0

-0.200913

-

0

0

N

i

CytoplasmicMembrane

10

67

CHEA_ECOLI

P07363

cheA

b1888 JW1877

cytoplasmic peripheral IM protein, Chemotaxis CheA

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)

[19766000]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

IM (predicted), Soluble IM Fraction +

Cytoplasm

Cytoplasmic

no

yes

N

17

0.108

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

10

88

SYFB_ECOLI

P07395

pheT

b1713 JW1703

cytoplasmic peripheral IM protein, Phenylalanine--tRNA ligase beta subunit PheT

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

[23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasm

Cytoplasmic

yes

yes

N

29

0.131

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

9

79

TYPH_ECOLI

P07650

deoA

tpp ttg

b4382 JW4345

cytoplasmic peripheral IM protein, Thymidine phosphorylase DeoA

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

[23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

IM (predicted)

Cytoplasmic

no

yes

N

53

0.104

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

8

12

FHUC_ECOLI

P07821

fhuC

b0151 JW0147

cytoplasmic peripheral IM protein, Iron(3+)-hydroxamate import ATP-binding FhuC

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)

Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279]

[1551849] [23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cell inner membrane

Cytoplasmic

yes

yes

N

39

0.111

CYT

0

-0.200913

-

0

0

N

i

CytoplasmicMembrane

10

45

IDH_ECOLI

P08200

icd

icdA icdE

b1136 JW1122

cytoplasmic peripheral IM protein, Isocitrate dehydrogenase (NADP) Icd

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

Trigger factor-sensitive aggregation.

[23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

IM (predicted)

Cytoplasmic

TF

yes

yes

N

47

0.119

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

9

96

SYFA_ECOLI

P08312

pheS

b1714 JW5277

cytoplasmic peripheral IM protein, Phenylalanine--tRNA ligase alpha subunit PheS

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

[23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasm

Cytoplasmic

yes

yes

N

19

0.166

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

10

12

DNAJ_ECOLI

P08622

dnaJ

groP

b0015 JW0014

cytoplasmic peripheral IM protein, Chaperone DnaJ

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

Interacts with DmsD is a redox enzyme maturation protein (REMP). [20153451]

[20153451] [23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasm

Cytoplasmic

yes

yes

N

39

0.105

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

10

52

PT1_ECOLI

P08839

ptsI

b2416 JW2409

cytoplasmic peripheral IM protein, Phosphoenolpyruvate-phosphotransferase PtsI

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)

[23230279] [2644649]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasm

Cytoplasmic

yes

yes

N

15

0.115

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

10

90

NARG_ECOLI

P09152

narG

bisD narC

b1224 JW1215

cytoplasmic peripheral IM protein, Respiratory nitrate reductase 1 alpha chain NarG

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

Part of the nitrate reductase A (NarGHI) of Escherichia coli which is membrane anchored through the NarI subunit. NarH is the electron transfer subunit. [12910261] In vivo associations of Escherichia coli NarJ with a peptide of the first 50 residues of nitrate reductase catalytic subunit NarG. [19295650] When E. coli is grown anaerobically in the presence of high levels of nitrate, the respiratory chain that is formed is terminated by NarGHI otherwise known as the quinol nitrate oxidoreductase. [2674654] The heterotrimeric NarGHI has an ?????? structure encoded by narGHI respectively. The ?? and ?? subunits can be purified as a soluble NarGH dimer in the cytoplasm showing that these 2 subunits associate to form a stable subcomplex before association with the membrane ?? subunit. [3371362] The NarGH subcomplex is anchored to the cytoplasmic side of the membrane by NarI. NarJ interacts with apoNarGH early on, promoting the insertion of the [4Fe???S] clusters and molybdenum in a sequential manner. Insertion of the [Fe???S] clusters in NarH are not dependent on NarJ. Binding of NarJ also prevents premature targeting of the NarGH dimer to the membrane. [16286471] Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279]

[12910261] [16286471] [23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

Soluble IM Fraction

Cell membrane; Peripheral membrane protein

Cytoplasmic

yes

yes

N

20

0.124

CYT

0

-0.200913

-

0

0

N

i

CytoplasmicMembrane

10

29

GRPE_ECOLI

P09372

grpE

b2614 JW2594

cytoplasmic peripheral IM protein, GrpE

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

Interacts with DmsD is a redox enzyme maturation protein (REMP). [20153451]

[20153451] [23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasm

Cytoplasmic

no

yes

N

35

0.101

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

9

PFLB_ECOLI

P09373

pflB

pfl

b0903 JW0886

cytoplasmic peripheral IM protein, Formate acetyltransferase 1 PflB

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

Trigger factor-sensitive aggregation.

[23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

Soluble IM Fraction

Cytoplasm

Cytoplasmic

TF

yes

yes

N

48

0.103

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

10

30

PUTA_ECOLI

P09546

putA

poaA

b1014 JW0999

cytoplasmic nucleoid peripheral IM protein, Bifunctional PutA

Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (By similarity) Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (Experimental)

The proline dehydrogenase PutA protein from S. typhimurium was shown to act as a membrane-associated enzyme or as a transcriptional repressor [8483946]. Catabolism of proline requires PutA to associate with the membrane because reoxidation of the FAD cofactor in PutA needs the presence of an electron acceptor. Repression of the put operon requires PutA to bind to the put control-region DNA in the cytoplasm. Transcription factor [23138451]. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279]

[23138451] [23230279] [8483946]

Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm

A, N, F1

Soluble IM Fraction

Cytoplasmic

yes

yes

N

64

0.118

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

9

GLTB_ECOLI

P09831

gltB

aspB

b3212 JW3179

cytoplasmic peripheral IM protein, Glutamate synthase (NADPH) large chain GltB

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

Disulfide bond-containing protein [25664544]

[23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasmic

no

yes

N

48

0.135

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

9

MODC_ECOLI

P09833

modC

chlD narD

b0765 JW0748

cytoplasmic peripheral IM protein, Molybdenum import ATP-binding ModC

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

Soluble IM Fraction

Cell inner membrane; Peripheral membrane protein

Cytoplasmic

no

yes

N

35

0.128

CYT

0

-0.200913

-

0

0

N

i

CytoplasmicMembrane

10

UVRA_ECOLI

P0A698

uvrA

dinE

b4058 JW4019

cytoplasmic peripheral IM protein, UvrABC system A UvrA

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (By similarity)

Homolog to import ATP-binding protein MalK. Trigger factor-sensitive aggregation. Copurifies with trigger factor in pull down assays.

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

Soluble IM Fraction

Cytoplasm

Cytoplasmic

TF

no

yes

N

42

0.254

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

9

ACKA_ECOLI

P0A6A3

ackA

ack

b2296 JW2293

cytoplasmic peripheral IM protein, Acetate kinase AckA

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

[23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasm

Cytoplasmic

yes

yes

N

20

0.328

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

10

112

ACP_ECOLI

P0A6A8

acpP

b1094 JW1080

cytoplasmic peripheral IM protein, Acyl carrier AcpP

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)

Part of phospholipid metabolism complex PlsB-PssA-YbgC-Acp[16294310]. Various methods were used to demonstrate that ACP could be cross-linked specifically to an inner membrane protein of E. coli, most probably to the glycerol-3-phosphate acyl transferase (GPAT)[1310877]. Evidence for interactions of acyl carrier protein with glycerol-3-phosphate acyltransferase, an inner membrane protein of Escherichia coli[2676605]. Membrane vesicles of Escherichia coli contain protein-binding sites for acyl carrier protein[2668033]. [3884600] Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279]

[1310877] [23230279] [2668033] [2676605] [3884600]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasm

Cytoplasmic (Experimental)

pseudogene (Ochman & Davalos, 2006)

no

yes

N

47

0.119

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

10

ISCS_ECOLI

P0A6B7

iscS

nuvC yfhO yzzO

b2530 JW2514

cytoplasmic peripheral IM protein, Cysteine desulfurase IscS

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

IscS is critical for iron-sulfur (Fe-S) cluster formation and repair. IscS transfers sulfur to the coregulated "scaffold" protein IscU (NifU), on which Fe-S clusters are assembled.

[23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasm

Cytoplasmic

yes

yes

N

48

0.104

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

9

37

ARGB_ECOLI

P0A6C8

argB

b3959 JW5553

cytoplasmic peripheral IM protein, Acetylglutamate kinase ArgB

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

[23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasm

Cytoplasmic

yes

yes

N

22

0.115

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

9

81

ASSY_ECOLI

P0A6E4

argG

b3172 JW3140

cytoplasmic peripheral IM protein, Argininosuccinate synthase ArgG

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

[23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasm

Cytoplasmic

yes

yes

N

37

0.163

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

9

19

ATPE_ECOLI

P0A6E6

atpC

papG uncC

b3731 JW3709

cytoplasmic peripheral IM protein, ATP synthase epsilon chain AtpC

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)

[16079137] [21778229] [23230279] [9868784]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

Soluble IM Fraction

Cell inner membrane

Cytoplasmic (Experimental)

no

yes

N

41

0.107

CYT

0

-0.200913

-

0

0

N

i

CytoplasmicMembrane

10

120

CARA_ECOLI

P0A6F1

carA

pyrA

b0032 JW0030

cytoplasmic peripheral IM protein, Carbamoyl-phosphate synthase small chain CarA

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

Copurifies with trigger factor in pull down assays.

[23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasmic

TF

yes

yes

N

22

0.124

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

9

60

GLPK_ECOLI

P0A6F3

glpK

b3926 JW3897

cytoplasmic peripheral IM protein, Glycerol kinase GlpK

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

Trigger factor-sensitive aggregation.

[23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasmic

TF

yes

yes

N

22

0.143

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

9

21

CH60_ECOLI

P0A6F5

groL

groEL mopA

b4143 JW4103

cytoplasmic peripheral IM protein, 60 kDa chaperonin GroL

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

Identified in nucleoids collected by sucrose gradient centrifugation and analyzed by liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS). [21541338]

[23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

Soluble IM Fraction

Cytoplasm

Cytoplasmic

yes

yes

N

58

0.12

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

10

94

CH10_ECOLI

P0A6F9

groS

groES mopB

b4142 JW4102

cytoplasmic peripheral IM protein, 10 kDa chaperonin GroS

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

[20153451] [22380631] [23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasm

Cytoplasmic

no

yes

N

43

0.185

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

10

86

HSLU_ECOLI

P0A6H5

hslU

htpI

b3931 JW3902

cytoplasmic peripheral IM protein, ATP-dependent protease ATPase subunit HslU

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

[23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasm

Cytoplasmic

yes

yes

N

24

0.141

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

10

74

DADA_ECOLI

P0A6J5

dadA

dadR

b1189 JW1178

cytoplasmic peripheral IM protein, D-amino acid dehydrogenase DadA

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)

Trigger factor-sensitive aggregation. Copurifies with trigger factor in pull down assays. Exhibites aberrant localization by microscopy in the absence of YidC. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279]

[23230279] [6102989]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

Soluble IM Fraction

Cell inner membrane

Cytoplasmic

TF

yes

yes

N

23

0.155

TMH

0

0.264511

-

0

0

Y

n3-11c16/17o

CytoplasmicMembrane

10

12

DEF_ECOLI

P0A6K3

def

fms

b3287 JW3248

cytoplasmic peripheral IM protein, Peptide deformylase Def

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

[23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasmic

yes

yes

N

42

0.113

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

9

66

DEOB_ECOLI

P0A6K6

deoB

drm thyR

b4383 JW4346

cytoplasmic peripheral IM protein, Phosphopentomutase DeoB

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

[23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

Soluble IM Fraction

Cytoplasm

Cytoplasmic

yes

yes

N

40

0.147

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

10

101

DEOC_ECOLI

P0A6L0

deoC

dra thyR

b4381 JW4344

cytoplasmic peripheral IM protein, Deoxyribose-phosphate aldolase DeoC

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

[23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasm

Cytoplasmic

yes

yes

N

22

0.136

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

10

115

EFG_ECOLI

P0A6M8

fusA

far fus

b3340 JW3302

cytoplasmic peripheral IM protein, Elongation factor G FusA

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

Translation [7042386]

[23230279] [7042386]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasm

Cytoplasmic (Experimental)

yes

yes

N

39

0.11

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

9

62

EFTS_ECOLI

P0A6P1

tsf

b0170 JW0165

cytoplasmic peripheral IM protein, Elongation factor Ts Tsf

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

Translation Identified in nucleoids collected by sucrose gradient centrifugation and analyzed by liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS). [21541338]

[23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasm

Cytoplasmic

no

yes

N

59

0.109

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

10

87

DER_ECOLI

P0A6P5

der

engA yfgK

b2511 JW5403

ribosomal cytoplasmic peripheral IM protein, GTPase Der

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm & ribosomal (Experimental)

Essential protein, proposed to be engaged in a functional cyclic interaction between ribosomes and the membrane in Escherichia coli through interaction with the 50S subunit. Assembly cofactor of ribosome; associates with the 50S subunit and YihI. YeaA has been viewed through fluorescence microscopy and located evenly throughout cytoplasm. Small scale study of labeling chromosomal coding DNA sequences (CDSs) with fluorescent protein genes. [17272300] Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279]

[17272300] [20172997] [21354313] [23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm, ribosomal

A, F1, r

N/A

Cytoplasmic

yes

yes

N

31

0.139

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

9

66

ENGB_ECOLI

P0A6P7

engB

yihA

b3865 JW5930

cytoplasmic peripheral IM protein, Probable GTP-binding EngB

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

Fluorescent fusions of YqiK have been monitored via fluorescence microscopy. YqiK was found to be distributed diffusely throughout cytoplasm.

[17272300] [23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasmic

yes

yes

N

43

0.126

CYT

0

-0.200913

-

0

0

N

i

Unknown

2

ENO_ECOLI

P0A6P9

eno

b2779 JW2750

cytoplasmic peripheral IM protein facing the cytoplasm & peripheral OM protein facing the extra-cellular space (SEC through IM), Enolase Eno

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm, Peripheral outer membrane protein facing the extra-cellular space (Experimental) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm, Peripheral outer membrane protein facing the extra-cellular space (Potential) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm, Peripheral outer membrane protein facing the extra-cellular space (Probable)

When modified at Lys342 possibly secreted by an unknown mechanism, no evidence of signal peptide) Homodimer, stabilized by Mg2+; Catalyzes the reversible conversion of 2-phosphoglycerate into phosphoenolpyruvate. Essential for the degradation of carbohydrates via glycolysis. Also a component of the RNA degradosome. The covalent binding to the substrate at Lys-342 causes inactivation of the enzyme, and possibly serves as a signal for the export of the protein. Interacts with the C-terminal region of the endoribonuclease RNase EIn the RNA degradosome. Fractions of enolase are present in both the cytoplasm and on the cell surface. As part of the bacterial cytoskeleton in the cytoplasm,Is organized as extended coiled structures that wind around the cell, from one cell pole to the other. When covalently bound to the substrate at Lys-342, the inactive enolase is secreted, and remains attached to the bacterial cell surface. [8610017] [9600841] [9740056] [9732527] [12035760] Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279]

[12035760] [15003462] [17242352] [18337249] [23230279] [8610017] [9600841] [9732527] [9740056]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm, Peripheral outer membrane protein facing the extra-cellular space

A, F1, F4

Soluble IM Fraction

Cytoplasm; cytoskeleton Secreted Cell surface

Cytoplasmic (Experimental)

SEC

yes

yes

N

42

0.139

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

9

100

FABZ_ECOLI

P0A6Q6

fabZ

sefA yaeA

b0180 JW0175

cytoplasmic peripheral IM protein, 3-hydroxyacyl-(acyl-carrier-protein) dehydratase FabZ

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)

Trigger factor-sensitive aggregation. [8354462] [7806516] [8805534] [8910376] Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279]

[15911532] [23230279] [7806516] [8354462] [8805534] [8910376]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasm

Cytoplasmic (Experimental)

TF

yes

yes

N

32

0.104

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

10

27

FIS_ECOLI

P0A6R3

fis

b3261 JW3229

cytoplasmic nucleoid peripheral IM protein, DNA-binding Fis

Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (Experimental)

Nucleoid-associated protein (NAP).

[21541338] [23230279]

Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm

A, N, F1

N/A

Cytoplasm; nucleoid

Cytoplasmic

yes

yes

N

19

0.116

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

9

110

MNMG_ECOLI

P0A6U3

mnmG

gidA trmF

b3741 JW3719

cytoplasmic peripheral IM protein, tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)

Similar localization to mnmE; may form a complex together

[17062623]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasm

Cytoplasmic

yes

yes

N

25

0.172

CYT

0

-0.200913

-

0

1

N

o601-619i

Cytoplasmic

9

6

GLGA_ECOLI

P0A6U8

glgA

b3429 JW3392

cytoplasmic peripheral IM protein, Glycogen synthase GlgA

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

[23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasmic

yes

yes

N

32

0.162

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

9

10

HEM1_ECOLI

P0A6X1

hemA

b1210 JW1201

cytoplasmic peripheral IM protein, Glutamyl-tRNA reductase HemA

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

Translation unit

[23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasmic

yes

yes

N

44

0.116

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

9

23

HFQ_ECOLI

P0A6X3

hfq

b4172 JW4130

cytoplasmic nucleoid peripheral IM protein, RNA-binding Hfq

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm, Nucleoid (Experimental)

The Escherichia coli hflA locus encodes a putative GTP-binding protein and two membrane proteins, one of which contains a protease-like domain. Is a bacterial protein involved in several aspects of nucleic acid transactions. Nucleoid-associated protein (NAP) that binds sequence-nonspecific DNA. Part of membrane-associating assemblies that coil around the periphery of the cell when visualized by immunofluorescence microscopy. These assemblies co-localize with the RNA degradosome and participate in the RNA degradation process. [24438330]

[20011543] [21541338] [24438330]

Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm

A, N, F1

N/A

Cytoplasmic

yes

yes

N

53

0.107

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

10

75

IHFA_ECOLI

P0A6X7

ihfA

hid himA

b1712 JW1702

cytoplasmic nucleoid peripheral IM protein, Integration host factor subunit alpha IhfA

Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (Experimental) Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (Potential)

Translation. Experimentally verified gene-product. Methionine start codon is cleaved. Identified in nucleoids collected by sucrose gradient centrifugation and analyzed by liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS)[21541338]. Bacterial histone-like DNA-binding proteins signature

[21541338] [23230279]

Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm

A, N, F1

N/A

Cytoplasm; nucleoid

Cytoplasmic

no

yes

N

24

0.109

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

9

IHFB_ECOLI

P0A6Y1

ihfB

himD hip

b0912 JW0895

cytoplasmic nucleoid peripheral IM protein, Integration host factor subunit beta IhfB

Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (Experimental) Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (Potential)

Translation, nucleoid-associated protein (NAP). Bacterial histone-like DNA-binding proteins signature

[21541338] [23230279]

Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm

A, N, F1

N/A

Cytoplasm

Cytoplasmic

no

yes

N

39

0.116

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

9

23

HSLO_ECOLI

P0A6Y5

hslO

yrfI

b3401 JW5692

cytoplasmic peripheral IM protein, 33 kDa chaperonin HslO

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

Disulfide bridge (230 232;263 266) [10764757]

[23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

IM (predicted)

Cytoplasm

Cytoplasmic

yes

yes

N

63

0.125

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

10

88

DNAK_ECOLI

P0A6Y8

dnaK

groP grpF seg

b0014 JW0013

cytoplasmic peripheral IM protein of Hsp70 family, Chaperone DnaK

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)

DnaK associates with the inner membrane. The ability to isolate DnaK with a membrane fraction prepared under gentle lysis conditions supports a peripheral association between DnaK and the cytoplasmic membrane. [7961473] Overexpression of three different membrane proteins (YidC, YedZ, and LepI) fused to green fluorescent protein (GFP) led to an increased association (2.3???4.8-fold) of DnaK with the membrane. [17446557] [1396676] [7783627] Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279] Interacts with DmsD is a redox enzyme maturation protein (REMP). [20153451]

[1396676] [16079137] [17446557] [20153451] [23230279] [7783627]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

Soluble IM Fraction

Cytoplasm

Cytoplasmic (Experimental)

yes

yes

N

20

0.127

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

10

115

HTPG_ECOLI

P0A6Z3

htpG

b0473 JW0462

cytoplasmic peripheral IM protein, Chaperone HtpG

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)

Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279]

[16079137] [23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

Soluble IM Fraction

Cytoplasm

Cytoplasmic

yes

yes

N

43

0.189

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

10

109

IF2_ECOLI

P0A705

infB

gicD ssyG

b3168 JW3137

ribosomal cytoplasmic peripheral IM protein, Translation initiation factor IF-2 InfB

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm & ribosomal (Experimental)

Translation. Trigger factor-sensitive aggregation.

[23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm, ribosomal

A, F1, r

N/A

Cytoplasm

Cytoplasmic

TF

yes

yes

N

28

0.114

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

9

65

IF3_ECOLI

P0A707

infC

fit srjA

b1718 JW5829

ribosomal cytoplasmic peripheral IM protein, Translation initiation factor IF-3 InfC

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm & ribosomal (Experimental)

Translation. Trigger factor-sensitive aggregation. Evidence that the inter-domain region is disordered in solution.

[23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm, ribosomal

A, F1, r

N/A

Cytoplasm

Cytoplasmic

TF

no

yes

N

22

0.117

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

10

113

KPRS_ECOLI

P0A717

prs

prsA

b1207 JW1198

cytoplasmic peripheral IM protein, Ribose-phosphate pyrophosphokinase Prs

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

Trigger factor-sensitive aggregation.

[23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasm

Cytoplasmic

TF

yes

yes

N

31

0.131

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

10

9

MINE_ECOLI

P0A734

minE

b1174 JW1163

cytoplasmic peripheral IM protein, Cell division topological specificity factor MinE

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)

MinD recruits MinC to the membrane to form the MinC-MinD complex, which inhibits septum formation, and MinE acts to localize and restrict inhibition to the inappropriate septation sites. The N-Terminal Amphipathic Helix of the Topological Specificity Factor MinE Is Associated with Shaping Membrane Curvature. [21738659]

[10096083] [20025670] [21738659]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasmic

yes

yes

N

18

0.123

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

9

101

KEFF_ECOLI

P0A754

kefF

yabF

b0046 JW0045

cytoplasmic peripheral IM protein, Glutathione-regulated potassium-efflux system ancillary KefF

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)

Properties of four two-component bacterial transport systems of the cation/proton antiporter-2 (CPA2) family led to suggestions that this CPA2 subset may use a channel rather than an antiport mechanism. The transporter subset includes the intensively studied glutathione-gated K+ efflux systems from <i>Escherichia coli</i>, KefGB, and KefFC. KefG and KefF are ancillary proteins. They are peripheral membrane proteins that are encoded in operons with the respective transporter proteins, KefB and KefC, and are required for optimal efflux activity.

[17679694]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

IM (predicted)

Cell inner membrane

Cytoplasmic

yes

yes

N

17

0.127

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

9

34

KEFG_ECOLI

P0A756

kefG

yheR

b3351 JW3314

cytoplasmic peripheral IM protein, Glutathione-regulated potassium-efflux system ancillary KefG

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)

Properties of four two-component bacterial transport systems of the cation/proton antiporter-2 (CPA2) family led to suggestions that this CPA2 subset may use a channel rather than an antiport mechanism. The transporter subset includes the intensively studied glutathione-gated K+ efflux systems from Escherichia coli, KefGB, and KefFC. KefG and KefF are ancillary proteins. They are peripheral membrane proteins that are encoded in operons with the respective transporter proteins, KefB and KefC, and are required for optimal efflux activity. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279]

[17679694] [23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

IM (predicted)

Cell inner membrane

Cytoplasmic

yes

yes

N

23

0.13

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

9

NAGB_ECOLI

P0A759

nagB

glmD

b0678 JW0664

cytoplasmic peripheral IM protein, Glucosamine-6-phosphate deaminase NagB

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

Disulfide bridge (219 219) [8240271]

[23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

IM (predicted)

Cytoplasmic

yes

yes

N

32

0.107

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

8

28

NDK_ECOLI

P0A763

ndk

b2518 JW2502

cytoplasmic peripheral IM protein, Nucleoside diphosphate kinase Ndk

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (By similarity) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)

Nucleoside diphosphate kinase (EC 2.7.4.6) (Ndk) is a ubiquitous enzyme functioning in the intracellular distribution of terminal phosphate bond energy among the various nucleotides used in synthetic and regulatory functions in cells[8606147]. Based on studies in Pseudomonas and many other systems including mitochondria, the uracil-processing DNA repair nuclease ndk is known to interact to plasma membranes and mitochondrial inner membrane[15096615]. Binding is regulated by nucleotides[19146889]. In Pseudomonas, an intracellular protease cleaves the 16-kDa form of Ndk to a 12-kDa form that undergoes autophosphorylation with an efficiency almost identical to that of the 16-kDa form. The 12-kDa form is predominantly associated with the cell membrane fraction, whereas the 16-kDa form is predominantly cytoplasmic. In the membrane-associated state, the 12-kDa form of Ndk was found to synthesize GTP in preference to other nucleoside triphosphates. The formation of the 12-kDa form of Ndk and its association with the cell membrane were found to be related to the growth stage of <i>P.aeruginosa</i>, with less than 1% of the 12-kDa Ndk detectable in the membrane fraction at early log phase in comparison with the levels present at late stationary phase[8606147]. Cytosolic Ndks bind directly to membrane lipids in a dynamic process and can be fully reconstituted with phospholipids and recombinant Ndk [19146889]. Ndks may act as a reservoir of high energy phosphate, supporting the operation of membrane-based processes that utilize nucleotides other than ATP, such as intracellular traffic and phospholipid biosynthesis. Trigger factor-sensitive aggregation[19737520]. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279]

[19146889] [23230279] [8606147]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasm

Cytoplasmic

TF

no

yes

N

17

0.158

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

10

64

RPPH_ECOLI

P0A776

rppH

nudH ygdP

b2830 JW2798

cytoplasmic nucleoid peripheral IM protein, RNA pyrophosphohydrolase RppH

Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (Experimental)

Part of membrane-associating assemblies that coil around the periphery of the cell when visualized by immunofluorescence microscopy. These assemblies co-localize with the RNA degradosome and participate in the RNA degradation process. [24438330]

[24438330]

Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm

A, N, F1

N/A

Cytoplasmic

yes

yes

N

25

0.126

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

9

62

ORN_ECOLI

P0A784

orn

o204a yjeR

b4162 JW5740

cytoplasmic peripheral IM protein, Oligoribonuclease Orn

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

Part of membrane-associating assemblies that coil around the periphery of the cell when visualized by immunofluorescence microscopy. These assemblies co-localize with the RNA degradosome and participate in the RNA degradation process. [24438330]

[24438330]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasm

Cytoplasmic

yes

yes

N

48

0.121

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

9

103

PFKA_ECOLI

P0A796

pfkA

b3916 JW3887

cytoplasmic peripheral IM protein, ATP-dependent 6-phosphofructokinase isozyme 1 PfkA

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

[23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasm

Cytoplasmic

yes

yes

N

20

0.426

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

10

76

PGK_ECOLI

P0A799

pgk

b2926 JW2893

cytoplasmic peripheral IM protein, Phosphoglycerate kinase Pgk

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

[23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasm

Cytoplasmic

yes

yes

N

21

0.107

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

10

100

PLSB_ECOLI

P0A7A7

plsB

b4041 JW4001

cytoplasmic peripheral IM protein, Glycerol-3-phosphate acyltransferase PlsB

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)

Phospholipid metabolism complex PlsB-PssA-YbgC-acp Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279]

[15919657] [16294310] [23230279] [4943977]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

Soluble IM Fraction

Cell inner membrane

Cytoplasmic

no

yes

N

27

0.194

CYT

0

-0.200913

-

0

0

N

i

CytoplasmicMembrane

10

PPK1_ECOLI

P0A7B1

ppk

b2501 JW2486

cytoplasmic peripheral IM protein, Polyphosphate kinase Ppk

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)

Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279]

[1331061] [23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cell membrane

Cytoplasmic

yes

yes

N

26

0.112

CYT

0

-0.200913

-

0

0

N

i

CytoplasmicMembrane

10

7

NADK_ECOLI

P0A7B3

nadK

yfjB yfjE

b2615 JW2596

cytoplasmic peripheral IM protein, NAD kinase NadK

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)

Fluorescent fusions of ppnK have been monitored via fluorescence microscopy. ppnK distributed irregularly throughout the cytoplasm, with some clusters formed.

[17272300]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasm

Cytoplasmic

yes

yes

N

20

0.111

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

9

32

PURA_ECOLI

P0A7D4

purA

adeK

b4177 JW4135

cytoplasmic peripheral IM protein, Adenylosuccinate synthetase PurA

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

[23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasm

Cytoplasmic

yes

yes

N

62

0.102

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

10

41

PUR7_ECOLI

P0A7D7

purC

b2476 JW2461

cytoplasmic peripheral IM protein, Phosphoribosylaminoimidazole-succinocarboxamide synthase PurC

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)

Experimentally detected by proteomics analysis of E.coli membrane fraction (Bernsel et al 2009). No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Potential peripherally associated protein facing the cytoplasm or integral inner membrane protein.

[19766000]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

Soluble IM Fraction

Cytoplasmic

yes

yes

N

65

0.127

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

9

50

PYRD_ECOLI

P0A7E1

pyrD

b0945 JW0928

cytoplasmic peripheral IM protein, Dihydroorotate dehydrogenase PyrD

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279]

[23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

Soluble IM Fraction

Cell membrane; Peripheral membrane protein

Cytoplasmic

yes

yes

N

45

0.146

CYT

0

-0.200913

-

0

0

N

i

CytoplasmicMembrane

10

12

PYRG_ECOLI

P0A7E5

pyrG

b2780 JW2751

cytoplasmic peripheral IM protein, CTP synthase PyrG

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)

PyrG=CTP synthase or CtpS in some systems. CTP synthase forms filaments in Caulobacter crescentus that regulate cell curvature independently of its catalytic function. The morphogenic role of CtpS requires its functional interaction with the intermediate filament, crescentin (CreS). Trigger factor-sensitive aggregation. [19737520] Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279]

[20639870] [23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

IM (predicted)

Cytoplasmic

TF

yes

yes

N

27

0.24

SpI

26

1.52462

-

1

0

N

i

Cytoplasmic

9

77

PYRH_ECOLI

P0A7E9

pyrH

smbA

b0171 JW0166

cytoplasmic peripheral IM protein, Uridylate kinase PyrH

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)

Fluorescent fusions of PyrH have been monitored via fluorescence microscopy. PyrH was found to be located in one membrane-associated foci per cell. [17272300] PyrH-EYFP fusions are predominantly localized near the bacterial membranes of different cells undergoing septation and division. [9922246] Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279]

[17272300] [23230279] [9922246]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasm

Cytoplasmic

yes

yes

N

23

0.14

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

9

30

RBFA_ECOLI

P0A7G2

rbfA

P15B yhbB

b3167 JW3136

ribosomal cytoplasmic peripheral IM protein, 30S ribosome-binding factor RbfA

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm & ribosomal (Experimental)

Assembly cofactor of ribosome; associate with the 30S subunit. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279]

[21529161] [23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasm

Cytoplasmic

yes

yes

N

51

0.152

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

10

68

RECA_ECOLI

P0A7G6

recA

lexB recH rnmB tif umuB zab

b2699 JW2669

cytoplasmic peripheral IM protein, RecA

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)

Protein of Escherichia coli binds specifically to acidic phospholipids such as cardiolipin and phosphatidylglycerol.The interaction of RecA protein with cardiolipin and phosphatidylglycerol, which represent two of the three major phospholipids of the E. coli membrane, may be physiologically important, as it provides a possible mechanism for the RecA-membrane association during the SOS response. Trigger factor-sensitive aggregation. Disulfide bond-containing protein [25664544]

[2228969]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

Soluble IM Fraction

Cytoplasm

Cytoplasmic

TF

no

yes

N

3

0.103

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

10

88

RECF_ECOLI

P0A7H0

recF

uvrF

b3700 JW3677

cytoplasmic nucleoid peripheral IM protein, DNA replication and repair RecF

Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (Potential) Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (Probable)

Experimentally detected by proteomics analysis of E.coli membrane fraction (Bernsel et al 2009). No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Potential peripherally associated protein facing the cytoplasm or integral inner membrane protein. The prokaryotic protein recF is a single-stranded DNA-binding protein which also probably binds ATP. RecF is involved in DNA metabolism; it is required for recombinational DNA repair and for induction of the SOS response. [1542576]

[1542576] [19766000]

Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm

A, N, F1

Soluble IM Fraction

Cytoplasm

Cytoplasmic

yes

yes

N

32

0.128

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

9

6

RIBB_ECOLI

P0A7J0

ribB

htrP

b3041 JW3009

cytoplasmic peripheral IM protein, 3,4-dihydroxy-2-butanone 4-phosphate synthase RibB

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cell membrane

Cytoplasmic

yes

yes

N

22

0.191

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

9

116

RIMM_ECOLI

P0A7X6

rimM

yfjA

b2608 JW5413

ribosomal cytoplasmic peripheral IM protein, Ribosome maturation factor RimM

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm & ribosomal (Experimental)

Assembly cofactor of ribosome; associate with the 30S subunit. [11514519] Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279]

[11514519] [23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm, ribosomal

A, F1, r

N/A

Cytoplasm

Cytoplasmic

yes

yes

N

39

0.124

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

9

106

RNC_ECOLI

P0A7Y0

rnc

b2567 JW2551

cytoplasmic nucleoid peripheral IM protein, Ribonuclease 3 Rnc

Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (Experimental) Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (Probable)

Copurifies with trigger factor in pull down assays. Part of membrane-associating assemblies that coil around the periphery of the cell when visualized by immunofluorescence microscopy. These assemblies co-localize with the RNA degradosome and participate in the RNA degradation process. [24438330]

[24438330] [4865702]

Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm

A, N, F1

N/A

Cytoplasm

Cytoplasmic

TF

yes

yes

N

56

0.133

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

10

116

RPOA_ECOLI

P0A7Z4

rpoA

pez phs sez

b3295 JW3257

cytoplasmic nucleoid peripheral IM protein, DNA-directed RNA polymerase subunit alpha RpoA

Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (Experimental) Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (Probable)

Identified in nucleoids collected by sucrose gradient centrifugation and analyzed by liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS). [21541338]

[21541338] [23230279]

Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm

A, N, F1

N/A

Cytoplasmic

yes

yes

N

25

0.163

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

9

RPOZ_ECOLI

P0A800

rpoZ

b3649 JW3624

cytoplasmic nucleoid peripheral IM protein, DNA-directed RNA polymerase subunit omega RpoZ

Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (Experimental)

The presence of omega subunit of RNA polymerase (RpoZ) is necessary for maturation of RNA polymerase. Experimentally verified gene-product. Methionine start codon is cleaved.

[23230279]

Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm

A, N, F1

N/A

Cytoplasmic

yes

yes

N

35

0.113

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

8

81

RRF_ECOLI

P0A805

frr

rrf

b0172 JW0167

cytoplasmic peripheral IM protein, Ribosome-recycling factor Frr

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

[23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasm

Cytoplasmic

yes

yes

N

33

0.103

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

10

109

METK_ECOLI

P0A817

metK

metX

b2942 JW2909

cytoplasmic peripheral IM protein, S-adenosylmethionine synthase MetK

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)

Fluorescent fusions of MetK have been monitored via fluorescence microscopy. Cells containing almost exclusively diffuse cytoplasmic MetK, or focused locations.

[17272300] [23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

Soluble IM Fraction

Cytoplasm

Cytoplasmic

yes

yes

N

64

0.118

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

10

47

GLYA_ECOLI

P0A825

glyA

b2551 JW2535

cytoplasmic peripheral IM protein, Serine hydroxymethyltransferase

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

[23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasm

Cytoplasmic

yes

yes

N

47

0.123

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

10

SUCC_ECOLI

P0A836

sucC

b0728 JW0717

cytoplasmic peripheral IM protein, Succinate--CoA ligase SucC

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

Trigger factor-sensitive aggregation.

[23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasmic

TF

yes

yes

N

32

0.181

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

8

67

TIG_ECOLI

P0A850

tig

b0436 JW0426

cytoplasmic peripheral IM protein, Trigger factor Tig

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

Translation unit [8633085] Interacts with DmsD is a redox enzyme maturation protein (REMP). [20153451]

[20153451] [23230279] [8633085]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasm

Cytoplasmic

yes

yes

N

21

0.128

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

8

87

TPIS_ECOLI

P0A858

tpiA

tpi

b3919 JW3890

cytoplasmic peripheral IM protein, Triosephosphate isomerase TpiA

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

[23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasm

Cytoplasmic

yes

yes

N

40

0.118

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

9

96

TPX_ECOLI

P0A862

tpx

yzzJ

b1324 JW1317

cytoplasmic peripheral IM protein, Thiol peroxidase Tpx

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

Binds to TrxA. although initially reported to be a periplasmic enzyme, the formation of a mixed-disulfide complex with TrxA indicates a cytoplasmic localization. [7499381] [9298646] Disulfide bond-containing protein [25664544]

[23230279] [7499381] [9298646]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Periplasm

Periplasmic (Experimental)

yes

yes

N

33

0.123

CYT

0

-0.200913

-

0

0

N

i

Periplasmic

10

TALB_ECOLI

P0A870

talB

yaaK

b0008 JW0007

cytoplasmic peripheral IM protein, Transaldolase B TalB

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

Trigger factor-sensitive aggregation.

[23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasm

Cytoplasmic

TF

yes

yes

N

33

0.122

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

8

84

RAPZ_ECOLI

P0A894

rapZ

yhbJ

b3205 JW3172

cytoplasmic peripheral IM protein, RNase adapter RapZ

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

[23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasmic

yes

yes

N

18

0.112

CYT

0

-0.200913

-

0

0

N

i

Unknown

7

38

RIMP_ECOLI

P0A8A8

rimP

yhbC

b3170 JW5533

cytoplasmic peripheral IM protein, Ribosome maturation factor RimP

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm ( Experimental)

Assembly cofactor of ribosome; associate with the 30S subunit.

[21529161]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasm

Cytoplasmic

yes

yes

N

19

0.107

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

9

111

YIDD_ECOLI

P0A8C8

yidD

b4557 JW3682

cytoplasmic peripheral IM protein, Putative insertion efficiency factor YidD

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)

[21803992]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cell inner membrane

Cytoplasmic

no

yes

N

42

0.11

CYT

0

-0.200913

-

0

0

N

i

Unknown

2

YIHI_ECOLI

P0A8H6

yihI

b3866 JW3837

cytoplasmic peripheral IM protein, Der GTPase-activating YihI

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

Assembly cofactor of ribosome; associates with Der.

[21529161]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasmic

yes

yes

N

43

0.101

CYT

0

-0.200913

-

0

0

N

i

Unknown

2

108

RHLB_ECOLI

P0A8J8

rhlB

mmrA

b3780 JW3753

cytoplasmic nucleoid peripheral IM protein, ATP-dependent RNA helicase RhlB

Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (Experimental)

[18337249] [23230279] [24438330]

Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm

A, N, F1

N/A

Cytoplasm

Cytoplasmic

yes

yes

N

56

0.132

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

9

PSD_ECOLI

P0A8K1

psd

b4160 JW4121

cytoplasmic peripheral IM protein, Phosphatidylserine decarboxylase proenzyme Psd

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

Phosphatidylserine decarboxylase is composed of two nonidentical subunits, alpha and beta, derived from a single proenzyme. The proenzyme is processed in a post-translational event into the heterodimer. This enzyme is unique among the known pyruvoyl-dependent decarboxylases because it is membrane-associated. [3042771] The phosphatidylserine decarboxylase (EC 4.1.1.65, phosphatidyl-L-serine carboxy-lyase) of Escherichia coli is associated with the cytoplasmic membrane and requires nonionic detergent for solubilization and detection of enzymatic activity [1495415] A strain of Escherichia coli bearing a hybrid plasmid containing the psd gene, starved for isoleucine by the addition of valine, produces amounts of phosphatidyl-serine decarboxylase, a membrane-bound enzyme, about 40-fold higher than wild type. [368058] The first phospholipid biosynthetic enzyme to be purified to homogeneity was Psd of E. coli. Beginning in the late 1960s purification of membrane proteins after solubilization with detergents was an increasingly popular endeavor. [22925633] [3042771] [5543671] Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279]

[1495415] [23230279] [3042771] [368058]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

Soluble IM Fraction

Cell membrane

Cytoplasmic (Experimental)

no

yes

N

23

0.185

CYT

0

-0.200913

-

0

0

N

i

CytoplasmicMembrane

10

32

SYS_ECOLI

P0A8L1

serS

b0893 JW0876

cytoplasmic peripheral IM protein, Serine--tRNA ligase SerS

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

Translation unit

[23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasm

Cytoplasmic

yes

yes

N

67

0.1

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

10

28

SYN_ECOLI

P0A8M0

asnS

tss

b0930 JW0913

cytoplasmic peripheral IM protein, Asparagine--tRNA ligase AsnS

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

Translation unit. Trigger factor-sensitive aggregation.

[23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasm

Cytoplasmic

TF

yes

yes

N

53

0.182

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

10

70

SYT_ECOLI

P0A8M3

thrS

b1719 JW1709

cytoplasmic peripheral IM protein, Threonine--tRNA ligase ThrS

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

Translation Trigger factor-sensitive aggregation.

[23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasm

Cytoplasmic

TF

yes

yes

N

33

0.154

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

10

22

MATP_ECOLI

P0A8N0

matP

ycbG

b0956 JW0939

cytoplasmic nucleoid peripheral IM protein, Macrodomain Ter protein MatP

Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (Probable)

[18984159]

Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm

A, N, F1

N/A

Cytoplasm

Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm

yes

yes

N

37

0.125

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

8

92

FETP_ECOLI

P0A8P3

yggX

b2962 JW2929

cytoplasmic peripheral IM protein, Probable Fe(2+)-trafficking protein YggX

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Potential) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)

Experimentally verified gene-product. Methionine start codon is cleaved. Experimentally detected by proteomics analysis of E.coli membrane fraction (Bernsel et al 2009). No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Potential peripherally associated protein facing the cytoplasm or integral inner membrane protein. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279]

[19766000] [23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

Soluble IM Fraction

Cytoplasmic

no

yes

N

41

0.14

CYT

0

-0.200913

-

0

0

Y

n4-11c16/17o

Unknown

2

107

RRAA_ECOLI

P0A8R0

rraA

menG yiiV

b3929 JW3900

cytoplasmic nucleoid peripheral IM protein, Regulator of ribonuclease activity A RraA

Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (Experimental)

Negative regulator of RNaseE. Part of membrane-associating assemblies that coil around the periphery of the cell when visualized by immunofluorescence microscopy. These assemblies co-localize with the RNA degradosome and participate in the RNA degradation process. [24438330]

[24438330]

Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm

A, N, F1

N/A

Cytoplasm

Cytoplasmic

no

yes

N

34

0.108

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

9

RPOC_ECOLI

P0A8T7

rpoC

tabB

b3988 JW3951

cytoplasmic nucleoid peripheral IM protein, DNA-directed RNA polymerase subunit beta' RpoC

Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (Experimental)

Trigger factor-sensitive aggregation.

[23230279]

Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm

A, N, F1

N/A

Cytoplasmic

TF

yes

yes

N

26

0.118

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

9

SYDP_ECOLI

P0A8U0

syd

ydr

b2793 JW2764

cytoplasmic peripheral IM protein, Syd

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279]

[23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cell inner membrane; Peripheral membrane protein; Cytoplasmic side

Cytoplasmic

yes

yes

N

20

0.125

CYT

0

-0.200913

-

0

0

N

i

CytoplasmicMembrane

10

59

RPOB_ECOLI

P0A8V2

rpoB

groN nitB rif ron stl stv tabD

b3987 JW3950

cytoplasmic nucleoid peripheral IM protein, DNA-directed RNA polymerase subunit beta RpoB

Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (Experimental)

Trigger factor-sensitive aggregation.

[23230279]

Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm

A, N, F1

N/A

Cytoplasmic

TF

yes

yes

N

40

0.117

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

9

YJGA_ECOLI

P0A8X0

yjgA

b4234 JW4193

cytoplasmic peripheral IM protein, UPF0307 YjgA

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm, ribosomal (Experimental)

[16980477] [23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm, ribosomal

A, F1, r

N/A

Cytoplasm

Cytoplasmic

yes

yes

N

46

0.101

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

9

81

YBGC_ECOLI

P0A8Z3

ybgC

b0736 JW0726

cytoplasmic peripheral IM protein, Acyl-CoA thioester hydrolase YbgC

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)

Phospholipid metabolism complex PlsB-PssA-YbgC-acp

[16294310]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cell inner membrane

Cytoplasmic

yes

yes

N

52

0.116

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

9

70

RIMJ_ECOLI

P0A948

rimJ

b1066 JW1053

cytoplasmic peripheral IM protein, Ribosomal-protein-alanine acetyltransferase RimJ

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

Assembly cofactor of ribosome; associate with the Pre-30S subunit. Overex<x>pression of RimJ suppresses defects in S5(G28D) mutations.

[23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasm

Cytoplasmic

yes

yes

N

33

0.107

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

10

FABB_ECOLI

P0A953

fabB

fabC

b2323 JW2320

cytoplasmic peripheral IM protein, 3-oxoacyl-(acyl-carrier-protein) synthase 1 FabB

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

[23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasm

Cytoplasmic

yes

yes

N

25

0.163

CYT

0

-0.200913

-

0

0

Y

n4-15c24/25o

Cytoplasmic

10

13

ALKH_ECOLI

P0A955

eda

hga kdgA

b1850 JW1839

cytoplasmic peripheral IM protein, KHG/KDPG aldolase Eda

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

[23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasm

Cytoplasmic

yes

yes

N

30

0.126

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

10

77

ALF1_ECOLI

P0A991

fbaB

dhnA

b2097 JW5344

cytoplasmic peripheral IM protein, Fructose-bisphosphate aldolase class 1 FbaB

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

Disulfide bond-containing protein [25664544]

[23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasm

Cytoplasmic

no

yes

N

64

0.115

CYT

0

-0.200913

-

0

0

N

i

Unknown

2

4

GLPC_ECOLI

P0A996

glpC

b2243 JW2237

cytoplasmic peripheral IM protein, Anaerobic glycerol-3-phosphate dehydrogenase subunit C GlpC

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)

Confirmed cytoplasmic membrane localization of GlpB. GlpB has been isolated from inner membrane preparations. Part of anaerobic glycerol-3-phosphate dehydrogenase composed of a catalytic GlpA/B dimer and of membrane bound GlpC. [3286606]

[3286606]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

Soluble IM Fraction

Cell inner membrane; Peripheral membrane protein

Membrane associated (Experimental)

yes

yes

N

35

0.222

CYT

0

-0.200913

-

0

0

N

i

CytoplasmicMembrane

10

13

FTNB_ECOLI

P0A9A2

ftnB

yecI

b1902 JW1890

cytoplasmic peripheral IM protein, Bacterial non-heme ferritin-like protein FtnB

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)

Experimentally detected by proteomics analysis of E.coli membrane fraction (Bernsel et al 2009). No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Potential peripherally associated protein facing the cytoplasm or integral inner membrane protein.

[19766000]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

Soluble IM Fraction

Cytoplasm

Cytoplasmic

yes

yes

N

48

0.152

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

10

82

FTSZ_ECOLI

P0A9A6

ftsZ

sfiB sulB

b0095 JW0093

cytoplasmic peripheral IM protein, Cell division FtsZ

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)

Essential cell division protein that forms a contractile ring structure (Z ring) at the future cell division site. Assembles at midcell at the inner surface of the cytoplasmic membrane. SulA halts cell division in Escherichia coli by binding to the major component of the division machinery FtsZ. [8917533] Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279] Over-expression of NlpI can interrupt the nucleoids division and the assembly of FtsZ at the septum, mediated by IbpA/IbpB, suggesting a role of the NlpI/IbpA/IbpB complex in the cell division. [25699035]

[1944597] [20497333] [23230279] [25699035] [8917533]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

Soluble IM Fraction

Cytoplasm

Cytoplasmic

yes

yes

N

26

0.12

CYT

0

-0.200913

-

0

0

N

i

CytoplasmicMembrane

10

79

G3P1_ECOLI

P0A9B2

gapA

b1779 JW1768

cytoplasmic peripheral IM protein, Glyceraldehyde-3-phosphate dehydrogenase A GapA

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

Trigger factor-sensitive aggregation. Disulfide bond-containing protein [25664544]

[23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

Soluble IM Fraction

Cytoplasm

Cytoplasmic

TF

yes

yes

N

21

0.121

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

9

73

GLPA_ECOLI

P0A9C0

glpA

b2241 JW2235

cytoplasmic peripheral IM protein, Anaerobic glycerol-3-phosphate dehydrogenase subunit A GlpA

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)

Confirmed cytoplasmic membrane localization of GlpB. GlpB has been isolated from inner membrane preparations. Part of anaerobic glycerol-3-phosphate dehydrogenase composed of a catalytic GlpA/B dimer and of membrane bound GlpC.

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

Soluble IM Fraction

Cell inner membrane; Peripheral membrane protein

Cytoplasmic

yes

yes

N

62

0.155

CYT

0

-0.200913

-

0

0

N

i

CytoplasmicMembrane

10

5

DAPD_ECOLI

P0A9D8

dapD

b0166 JW0161

cytoplasmic peripheral IM protein, 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase DapD

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

[23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasm

Cytoplasmic

yes

yes

N

24

0.195

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

10

41

CYSB_ECOLI

P0A9F3

cysB

b1275 JW1267

cytoplasmic nucleoid peripheral IM protein, HTH-type transcriptional regulator CysB

Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (Experimental)

[23138451] [23230279]

Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm

A, N, F1

IM (predicted)

Cytoplasm

Cytoplasmic

yes

yes

N

24

0.141

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

10

8

ACEA_ECOLI

P0A9G6

aceA

icl

b4015 JW3975

cytoplasmic peripheral IM protein, Isocitrate lyase AceA

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

Disulfide bond-containing protein [25664544]

[23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasmic

yes

yes

N

30

0.126

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

10

42

CFA_ECOLI

P0A9H7

cfa

cdfA

b1661 JW1653

cytoplasmic peripheral IM protein, Cyclopropane-fatty-acyl-phospholipid synthase Cfa

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

Exhibites aberrant localization by microscopy in the absence of YidC.

[23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasm

Cytoplasmic

yes

yes

N

38

0.183

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

10

26

CHEZ_ECOLI

P0A9H9

cheZ

b1881 JW1870

cytoplasmic peripheral IM protein, phosphatase CheZ

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)

Experimentally detected by proteomics analysis of E.coli membrane fraction (Bernsel et al 2009). No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Potential peripherally associated protein facing the cytoplasm or integral inner membrane protein. [12644507]

[12644507] [19766000]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

Soluble IM Fraction

Cytoplasm

Cytoplasmic

yes

yes

N

66

0.129

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

10

107

PHOL_ECOLI

P0A9K3

ybeZ

b0660 JW0657

cytoplasmic peripheral IM protein, PhoH-like protein YbeZ

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

Trigger factor-sensitive aggregation.

[23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasm

Cytoplasmic

TF

yes

yes

N

68

0.101

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

9

61

PHOU_ECOLI

P0A9K7

phoU

nmpA

b3724 JW3702

cytoplasmic peripheral IM protein, Phosphate-specific transport system accessory PhoU

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

Identified as peripheral membrane protein. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279]

[23230279] [3536855]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasm

Cytoplasmic

yes

yes

N

17

0.113

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

10

94

SLYD_ECOLI

P0A9K9

slyD

b3349 JW3311

cytoplasmic peripheral IM protein, FKBP-type peptidyl-prolyl cis-trans isomerase SlyD

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

[23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasm

Cytoplasmic

yes

yes

N

31

0.112

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

10

XGPT_ECOLI

P0A9M5

gpt

gpp gxu

b0238 JW0228

cytoplasmic peripheral IM protein, Xanthine phosphoribosyltransferase Gpt

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cell inner membrane

Cytoplasmic

yes

yes

N

48

0.21

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

10

DLDH_ECOLI

P0A9P0

lpdA

lpd

b0116 JW0112

cytoplasmic peripheral IM protein, Dihydroyl dehydrogenase LpdA

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)

Trigger factor-sensitive aggregation. [6352260] Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279] Disulfide bridge (45 50) Disulfide bond-containing protein [25664544]

[16079137] [23230279] [6352260]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

Soluble IM Fraction

Cytoplasm

Cytoplasmic (Experimental)

TF

yes

yes

N

23

0.176

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

10

79

DEAD_ECOLI

P0A9P6

deaD

csdA mssB rhlD

b3162 JW5531

cytoplasmic peripheral IM protein, ATP-dependent RNA helicase DeaD

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

Trigger factor-sensitive aggregation [19737520]. Contains a SPOR domain. [8552679]

[19880599] [23230279] [8552679]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasm

Cytoplasmic

TF

yes

yes

N

35

0.151

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

10

50

ARCA_ECOLI

P0A9Q1

arcA

cpxC dye fexA msp seg sfrA

b4401 JW4364

cytoplasmic nucleoid peripheral IM protein, Aerobic respiration control ArcA

Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (Experimental) Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (Probable)

[18792681] [23138451] [23230279]

Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm

A, N, F1

N/A

Cytoplasm

Cytoplasmic

yes

yes

N

28

0.105

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

10

90

ACCD_ECOLI

P0A9Q5

accD

dedB usg

b2316 JW2313

cytoplasmic peripheral IM protein, Acetyl-coenzyme A carboxylase carboxyl transferase subunit beta AccD

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

Translation related.

[23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

Soluble IM Fraction

Cytoplasm

Cytoplasmic

yes

yes

N

18

0.112

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

9

62

ADHE_ECOLI

P0A9Q7

adhE

ana

b1241 JW1228

cytoplasmic peripheral IM protein, Aldehyde-alcohol dehydrogenase AdhE

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)

Trigger factor-sensitive aggregation. Experimentally detected by proteomics analysis of E.coli membrane fraction (Bernsel et al 2009). No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Potential peripherally associated protein facing the cytoplasm or integral inner membrane protein.

[19766000]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

Soluble IM Fraction

Cytoplasmic

TF

yes

yes

N

56

0.127

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

9

45

DHAS_ECOLI

P0A9Q9

asd

hom

b3433 JW3396

cytoplasmic peripheral IM protein, Aspartate-semialdehyde dehydrogenase Asd

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Potential)

No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279]

[23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

IM (predicted-detected)

Cytoplasmic

yes

yes

N

44

0.197

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

9

18

FTSE_ECOLI

P0A9R7

ftsE

b3463 JW3428

cytoplasmic peripheral IM protein, Cell division ATP-binding FtsE

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

Homolog to import ATP-binding protein MalK. Is the putative ATP-binding protein component of an ATP-binding cassette (ABC) transporter. FtsE dimerizes and associates with the inner membrane via interaction with FtsX, an integral membrane protein. FtsE dimerizes and associates with the inner membrane via interaction with FtsX, an integral membrane protein. [10048040] Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279]

[10048040] [23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cell inner membrane

Cytoplasmic

yes

yes

N

32

0.118

CYT

0

-0.200913

-

0

0

N

i

CytoplasmicMembrane

10

11

GATD_ECOLI

P0A9S3

gatD

b2091 JW2075

cytoplasmic peripheral IM protein, Galactitol 1-phosphate 5-dehydrogenase GatD

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

Copurifies with trigger factor in pull down assays.

[23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

IM (predicted)

Cytoplasmic

TF

no

yes

N

53

0.115

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

9

53

LIVG_ECOLI

P0A9S7

livG

b3455 JW3420

cytoplasmic peripheral IM protein, High-affinity branched-chain amino acid transport ATP-binding LivG

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (By similarity)

Homolog to import ATP-binding protein MalK. Homolog to import ATP-binding protein MalK.

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasmic

yes

yes

N

21

0.325

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

9

11

YBBA_ECOLI

P0A9T8

ybbA

b0495 JW0484

cytoplasmic peripheral IM protein, ABC transporter ATP-binding YbbA

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (By similarity)

Homolog to import ATP-binding protein MalK

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasmic

yes

yes

N

41

0.105

CYT

0

-0.200913

-

0

0

N

i

CytoplasmicMembrane

10

102

YBHF_ECOLI

P0A9U1

ybhF

b0794 JW5104

cytoplasmic peripheral IM protein, Probable multidrug ABC transporter ATP-binding YbhF

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (By similarity)

Homolog to import ATP-binding protein MalK.

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

Soluble IM Fraction

Cytoplasmic

yes

yes

N

38

0.115

CYT

0

-0.200913

-

0

0

N

i

CytoplasmicMembrane

7

42

YBIT_ECOLI

P0A9U3

ybiT

b0820 JW0804

cytoplasmic peripheral IM protein, ABC transporter ATP-binding YbiT

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (By similarity) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

Homolog to import ATP-binding protein MalK. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279]

[23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasmic

yes

yes

N

19

0.108

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

9

42

LPTB_ECOLI

P0A9V1

lptB

yhbG

b3201 JW3168

cytoplasmic peripheral IM protein, polysaccharide export system ATP-binding LptB

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)

[16079137] [17056748] [23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

Soluble IM Fraction

Cytoplasm

Cytoplasmic(experimental)

yes

yes

N

43

0.117

CYT

0

-0.200913

-

0

0

N

i

CytoplasmicMembrane

10

19

ETTA_ECOLI

P0A9W3

ettA

yjjK

b4391 JW4354

cytoplasmic peripheral IM protein, Energy-dependent translational throttle EttA

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (By similarity) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

Homolog to import ATP-binding protein MalK. Trigger factor-sensitive aggregation. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279]

[23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasm

Cytoplasmic

TF

yes

yes

N

43

0.116

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

9

92

ZNUC_ECOLI

P0A9X1

znuC

yebM

b1858 JW1847

cytoplasmic peripheral IM protein, Zinc import ATP-binding ZnuC

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279]

[23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

Soluble IM Fraction

Cell inner membrane

Cytoplasmic

yes

yes

N

22

0.118

CYT

0

-0.200913

-

0

0

N

i

CytoplasmicMembrane

7

15

MREB_ECOLI

P0A9X4

mreB

envB rodY

b3251 JW3220

cytoplasmic peripheral IM protein, Cell shape-determining MreB

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)

Bacterial actin homologue; is required for cell shape maintenance in most non-spherical bacteria, where it assembles into helical structures just underneath the cytoplasmic membrane; binds to the bitopic inner membrane protein RodZ as monomer or multimer. Trigger factor-sensitive aggregation. Copurifies with trigger factor in pull down assays. [19737520] PBP-5 deletion mutants exhibit irregular distribution of MreB at the deformed zones. [PBP deletion mutants of Escherichia coli exhibit irregular distribution of MreB at the deformed zones. [24057063] [17090951] Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279]

[16391237] [17090951] [19008860] [19078962] [20168300] [21816350] [23230279] [24057063]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

Soluble IM Fraction

Cytoplasmic (Experimental)

TF

yes

yes

N

41

0.127

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

9

62

PTHP_ECOLI

P0AA04

ptsH

hpr

b2415 JW2408

cytoplasmic peripheral IM protein, Phosphocarrier HPr PtsH

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

[23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasm

Cytoplasmic

no

yes

N

22

0.11

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

10

88

OMPR_ECOLI

P0AA16

ompR

kmt ompB

b3405 JW3368

cytoplasmic nucleoid peripheral IM protein, transcriptional regulator OmpR

Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (Experimental)

[23138451] [23230279]

Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm

A, N, F1

Soluble IM Fraction

Cytoplasm

Cytoplasmic

yes

yes

N

38

0.124

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

10

87

THIO_ECOLI

P0AA25

trxA

fipA tsnC

b3781 JW5856

cytoplasmic peripheral IM protein, Thioredoxin 1 TrxA

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

Trigger factor-sensitive aggregation. Disulfide bridge (33 36) [10489448] Disulfide bond-containing protein [25664544]

[10489448] [23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasmic

TF

no

yes

N

20

0.119

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

9

89

CLSB_ECOLI

P0AA84

clsB

ybhO

b0789 JW0772

cytoplasmic peripheral IM protein, Cardiolipin synthase B ClsB

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

In E. coli, the cls and ybhO genes (renamed clsA and clsB, respectively) each encode a CL (cardiolipin) synthase (Cls) that catalyzes the condensation of two PG molecules to form CL and glycerol. [22988102]

[22988102]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cell membrane

Cytoplasmic

yes

yes

N

66

0.136

CYT

0

-0.200913

-

0

0

N

i

CytoplasmicMembrane

7

27

UBID_ECOLI

P0AAB4

ubiD

yigC yigY

b3843 JW3819

cytoplasmic peripheral IM protein, 3-octaprenyl-4-hydroxybenzoate carboxy-lyase UbiD

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279]

[23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

IM (predicted)

Cell membrane

Cytoplasmic

yes

yes

N

65

0.107

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

9

37

USPD_ECOLI

P0AAB8

uspD

yiiT

b3923 JW3894

cytoplasmic peripheral IM protein, Universal stress D UspD

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)

Experimentally detected by proteomics analysis of E.coli membrane fraction (Bernsel et al 2009). No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Potential peripherally associated protein facing the cytoplasm or integral inner membrane protein.

[19766000]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

Soluble IM Fraction

Cytoplasm

Cytoplasmic

yes

yes

N

54

0.108

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

9

96

ARAG_ECOLI

P0AAF3

araG

b1900 JW1888

cytoplasmic peripheral IM protein, Arabinose import ATP-binding AraG

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)

[7028715]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cell inner membrane

Cytoplasmic

yes

yes

N

38

0.293

CYT

0

-0.200913

-

0

0

N

i

CytoplasmicMembrane

10

37

ARTP_ECOLI

P0AAF6

artP

b0864 JW0848

cytoplasmic peripheral IM protein, Arginine transport ATP-binding ArtP

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (By similarity) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

The artPIQM artJ genes encode a third arginine-uptake system in addition to the known argT hisJQMP system of Salmonella typhimurium and E. coli and the arginine (-ornithine) carrier (aps) of E. coli. The artQ, artM and artP products are similar to the transmembraneous proteins and the ATPase of binding-protein-dependent carriers. [8801422] [8801422] [15758229] Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279]

[15758229] [23230279] [8801422]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

Soluble IM Fraction

Cell inner membrane

Cytoplasmic (Experimental)

yes

yes

N

20

0.124

CYT

0

-0.200913

-

0

0

N

i

CytoplasmicMembrane

7

26

DPPD_ECOLI

P0AAG0

dppD

b3541 JW3510

cytoplasmic peripheral IM protein, Dipeptide transport ATP-binding DppD

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279]

[23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

Soluble IM Fraction

Cell inner membrane

Cytoplasmic

yes

yes

N

66

0.286

CYT

0

-0.200913

-

0

0

N

i

CytoplasmicMembrane

7

19

GLTL_ECOLI

P0AAG3

gltL

b0652 JW0647

cytoplasmic peripheral IM protein, Glutamate/aspartate import ATP-binding GltL

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

Soluble IM Fraction

Cell inner membrane

Cytoplasmic

yes

yes

N

27

0.108

CYT

0

-0.200913

-

0

0

N

i

CytoplasmicMembrane

9

43

MGLA_ECOLI

P0AAG8

mglA

b2149 JW2136

cytoplasmic peripheral IM protein, Galactose/methyl galactoside import ATP-binding MglA

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

[15919657]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

Soluble IM Fraction

Cell inner membrane

Cytoplasmic

yes

yes

N

29

0.181

CYT

0

-0.200913

-

0

0

N

i

CytoplasmicMembrane

10

14

PSTB_ECOLI

P0AAH0

pstB

phoT

b3725 JW3703

cytoplasmic peripheral IM protein, Phosphate import ATP-binding PstB

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)

Identified as peripheral membrane protein. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279]

[23230279] [3881386]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

Soluble IM Fraction

Cell inner membrane; Peripheral membrane protein

Cytoplasmic

yes

yes

N

41

0.133

CYT

0

-0.200913

-

0

0

N

i

CytoplasmicMembrane

10

23

SAPD_ECOLI

P0AAH4

sapD

trkE

b1291 JW1284

cytoplasmic peripheral IM protein, Putrescine export system ATP-binding SapD

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279]

[23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cell inner membrane

Cytoplasmic

yes

yes

N

53

0.145

CYT

0

-0.200913

-

0

0

N

i

CytoplasmicMembrane

7

22

SAPF_ECOLI

P0AAH8

sapF

b1290 JW1283

cytoplasmic peripheral IM protein, Putrescine export system ATP-binding SapF

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279]

[23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cell inner membrane

Cytoplasmic

yes

yes

N

45

0.122

CYT

0

-0.200913

-

0

0

N

i

CytoplasmicMembrane

7

8

SSUB_ECOLI

P0AAI1

ssuB

ycbE

b0933 JW0916

cytoplasmic peripheral IM protein, Aliphatic sulfonates import ATP-binding SsuB

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cell inner membrane

Cytoplasmic

yes

yes

N

22

0.181

CYT

0

-0.200913

-

0

0

N

i

CytoplasmicMembrane

10

23

FABF_ECOLI

P0AAI5

fabF

fabJ

b1095 JW1081

cytoplasmic peripheral IM protein, 3-oxoacyl-(acyl-carrier-protein) synthase 2 FabF

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

[23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasmic

yes

yes

N

48

0.158

CYT

0

-0.200913

-

0

0

Y

n6-14c20/21o

Cytoplasmic

9

8

FDOH_ECOLI

P0AAJ5

fdoH

b3893 JW3864

cytoplasmic peripheral IM protein, Formate dehydrogenase-O iron-sulfur subunit FdoH

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Potential)

Part of FDH-O isoenzyme complex whose physiological role is to ensure rapid adaptation during a shift from aerobiosis to anaerobiosis. FDH-O is a membrane-bound enzyme complex composed of three subunits, alpha (FdoG), beta (FdoH), and gamma (FdoI). Identified in nucleoids collected by sucrose gradient centrifugation and analyzed by liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS). [21541338] Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279]

[21890895] [23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

IM (predicted)

Cell membrane; Single-pass membrane protein

Membrane anchored

yes

yes

N

24

0.119

CYT

0

-0.200913

-

0

0

N

i

CytoplasmicMembrane

10

77

YDHY_ECOLI

P0AAL6

ydhY

b1674 JW1664

cytoplasmic peripheral IM protein, Ferredoxin-like YdhY

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Potential)

No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius.

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

IM (predicted-detected)

Cytoplasmic

yes

yes

N

28

0.128

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

9

31

ALF_ECOLI

P0AB71

fbaA

fba fda

b2925 JW2892

cytoplasmic peripheral IM protein, Fructose-bisphosphate aldolase class 2 FbaA

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

Trigger factor-sensitive aggregation. [2649077]

[23230279] [2649077]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasmic (Experimental)

TF

yes

yes

N

46

0.11

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

9

69

KBL_ECOLI

P0AB77

kbl

b3617 JW3592

cytoplasmic peripheral IM protein, 2-amino-3-ketobutyrate coenzyme A ligase Kbl

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

Trigger factor-sensitive aggregation.

[23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasmic

TF

yes

yes

N

34

0.12

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

9

37

PUR8_ECOLI

P0AB89

purB

b1131 JW1117

cytoplasmic peripheral IM protein, Adenylosuccinate lyase PurB

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

[23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasmic

yes

yes

N

61

0.215

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

9

74

ATPD_ECOLI

P0ABA4

atpH

papE uncH

b3735 JW3713

cytoplasmic peripheral IM protein, ATP synthase subunit delta AtpH

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)

Identified in nucleoids collected by sucrose gradient centrifugation and analyzed by liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS). [21541338] Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279]

[15919657] [16079137] [23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

Soluble IM Fraction

Cell inner membrane

Cytoplasmic

no

yes

N

36

0.135

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

10

89

ATPG_ECOLI

P0ABA6

atpG

papC uncG

b3733 JW3711

cytoplasmic peripheral IM protein, ATP synthase gamma chain AtpG

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)

Identified in nucleoids collected by sucrose gradient centrifugation and analyzed by liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS). [21541338] [7508444] [2900236] Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279]

[16079137] [23230279] [2900236] [7508444]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

Soluble IM Fraction

Cell inner membrane

Cytoplasmic (Experimental)

yes

yes

N

41

0.162

CYT

0

-0.200913

-

0

0

N

i

CytoplasmicMembrane

10

48

ATPA_ECOLI

P0ABB0

atpA

papA uncA

b3734 JW3712

cytoplasmic peripheral IM protein, ATP synthase subunit alpha AtpA

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)

Trigger factor-sensitive aggregation. Exhibites aberrant localization by microscopy in the absence of YidC. Identified in nucleoids collected by sucrose gradient centrifugation and analyzed by liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS). [21541338] Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279]

[15919657] [16079137] [23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

IM (predicted)

Cell inner membrane

Cytoplasmic

TF

no

yes

N

28

0.106

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

10

60

ATPB_ECOLI

P0ABB4

atpD

papB uncD

b3732 JW3710

cytoplasmic peripheral IM protein, ATP synthase subunit beta AtpD

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

Exhibites aberrant localization by microscopy in the absence of YidC. Identified in nucleoids collected by sucrose gradient centrifugation and analyzed by liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS). [21541338] Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279]

[15919657] [23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

Soluble IM Fraction

Cell inner membrane; Peripheral membrane protein

Cytoplasmic

yes

yes

N

23

0.123

CYT

0

-0.200913

-

0

0

N

i

CytoplasmicMembrane

10

77

ACCA_ECOLI

P0ABD5

accA

b0185 JW0180

cytoplasmic peripheral IM protein, Acetyl-coenzyme A carboxylase carboxyl transferase subunit alpha AccA

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

[23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

Soluble IM Fraction

Cytoplasm

Cytoplasmic

yes

yes

N

27

0.103

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

10

28

PCNB_ECOLI

P0ABF1

pcnB

b0143 JW5808

cytoplasmic peripheral IM protein, PcnB

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)

RNA degradosome component/adds polyA tails to mRNAs targeted for degradation. The pcnB gene product, is the main enzyme responsible for RNA polyadenylation in E. coli. Polyadenylated RNA molecules are rapidly degraded by a multiprotein complex called RNA degradosome. PAP I localizes in the cytosol but also, like the RNA degradosome, in cellular membranes. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279]

[15737627] [23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

IM (predicted)

Cytoplasm

Cytoplasmic

yes

yes

N

26

0.111

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

9

23

FTSA_ECOLI

P0ABH0

ftsA

divA

b0094 JW0092

cytoplasmic peripheral IM protein, Cell division FtsA

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)

FtsA has a conserved C-terminal membrane targeting sequence (MTS) with extremely weak TmHMM and Phobius predictions. Deletion or point mutations in the MTS, inactivate FtsA function and inhibit cell division. Because FtsA binds to the tubulin-like FtsZ protein that forms the Z ring, it is thought that the MTS of FtsA is required, along with the transmembrane protein ZipA, to assemble the Z ring and anchor it to the cytoplasmic membrane. FtsA function in cell division depends on additive effects of membrane binding and self-interaction, and that the specific requirement of an amphipathic helix for tethering FtsA to the membrane can be bypassed. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279]

[18186792] [23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cell inner membrane

Cytoplasmic

yes

yes

N

25

0.12

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

9

84

CISY_ECOLI

P0ABH7

gltA

gluT icdB

b0720 JW0710

cytoplasmic peripheral IM protein, Citrate synthase GltA

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

Trigger factor-sensitive aggregation.

[23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

Soluble IM Fraction

Cytoplasmic

TF

yes

yes

N

43

0.113

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

9

22

CYSK_ECOLI

P0ABK5

cysK

cysZ

b2414 JW2407

cytoplasmic peripheral IM protein, Cysteine synthase A CysK

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Potential) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)

Trigger factor-sensitive aggregation. Experimentally detected by proteomics analysis of E.coli membrane fraction (Bernsel et al 2009). No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Potential peripherally associated protein facing the cytoplasm or integral inner membrane protein. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279]

[19766000] [23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

Soluble IM Fraction

Cytoplasmic

TF

yes

yes

N

31

0.116

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

9

77

DEOD_ECOLI

P0ABP8

deoD

pup

b4384 JW4347

cytoplasmic peripheral IM protein, Purine nucleoside phosphorylase DeoD-type DeoD

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

[23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasmic

yes

yes

N

27

0.108

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

9

108

DPS_ECOLI

P0ABT2

dps

pexB vtm

b0812 JW0797

cytoplasmic nucleoid peripheral IM protein, DNA protection during starvation Dps

Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (Experimental)

Nucleoid-associated protein (NAP) Dodecameric Dps forms a protein cage with a hollow interior that sequesters Fe 2+ and reduces hydroxyl radical formation [23701194] [7984106]

[21541338] [23230279] [7984106]

Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm

A, N, F1

Soluble IM Fraction

Cytoplasm; nucleoid

Cytoplasmic (Experimental)

yes

yes

N

53

0.125

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

10

44

FLIG_ECOLI

P0ABZ1

fliG

fla AII.2 fla BII

b1939 JW1923

Flagellar cytoplasmic peripheral IM protein, motor switch FliG

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

Flaggelar C-ring proteins - FliG, FliM, and FliN. MotA and MotB are ion-conducting membrane proteins that form the stator of the flagellum motor. FliG is a component of the rotor, present in about 25 copies per flagellum. The C-terminal domain of FliG is a component of the rotor in the bacterial flagellar motor. [10440379] [17085573] [16077109] [8631704]

[10440379] [16077109] [17085573] [8631704]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

Soluble IM Fraction

Cell inner membrane; Peripheral membrane protein; Cytoplasmic side Bacterial flagellum basal body

Membrane associated (Experimental)

flagellum

no

yes

N

23

0.251

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

9

80

KDSC_ECOLI

P0ABZ4

kdsC

yrbI yrbJ

b3198 JW3165

cytoplasmic peripheral IM protein, 3-deoxy-D-manno-octulosonate 8-phosphate phosphatase KdsC

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Potential)

Short signal peptide of 17aa. Prediction by Phobius and the SignalP NN approach/extensive islands of hydrophobicity by K/D. No obvious biochemical reason that the enzyme should be secreted. Signal peptide like sequence could act as peripheral membrane association element. Lipopolysaccharide (LPS) is an amphiphilic molecule composed of a hydrophilic heteropolysaccharide and the membrane-embedded Lipid A. The innermost 8-carbon sugar 3-deoxy-D-manno-oct-2-ulosonic acid (KDO) is the only conserved structural element found in all LPS, linking the lipid A to the carbohydrate domain of the molecule. The KDO 8-phosphate phosphatase (KdsC) is one of the enzymes of the KDO biosynthesis pathway and is responsible for cleavage of the phosphate group from KDO 8-phosphate to yield KDO. [12639950] In Escherichia coli, KdsC has been characterized as a homotetrameric molecule with a random-coiled C-terminal tail in each subunit. [12639950] [12639950]

[12639950]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasmic (Experimental)

yes

yes

N

18

0.326

CYT

0

-0.200913

-

0

0

Y

n4-12c17/18o

Cytoplasmic

9

90

FUMA_ECOLI

P0AC33

fumA

b1612 JW1604

cytoplasmic peripheral IM protein, Fumarate hydratase class I, aerobic FumA

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

Disulfide bond-containing protein [25664544]

[23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasmic

yes

yes

N

57

0.116

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

9

37

ASPA_ECOLI

P0AC38

aspA

b4139 JW4099

cytoplasmic peripheral IM protein, Aspartate ammonia-lyase AspA

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

Trigger factor-sensitive aggregation. Detected with chromosomally tap-tagged TF with >80% confidence score [15690043]

[23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasmic

TF

yes

yes

N

22

0.123

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

9

87

SDHA_ECOLI

P0AC41

sdhA

b0723 JW0713

cytoplasmic peripheral IM protein, Succinate dehydrogenase flavosubunit SdhA

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)

Trigger factor-sensitive aggregation. Copurifies with trigger factor in pull down assays. Identified in nucleoids collected by sucrose gradient centrifugation and analyzed by liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS). [21541338] [9600841] [9740056] Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279]

[12560550] [16079137] [23230279] [9600841] [9740056]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

Soluble IM Fraction

Cell inner membrane

Cytoplasmic (Experimental)

TF

yes

yes

N

50

0.189

CYT

0

-0.200913

-

0

0

N

i

CytoplasmicMembrane

10

10

FRDB_ECOLI

P0AC47

frdB

b4153 JW4114

cytoplasmic peripheral IM protein, Fumarate reductase iron-sulfur subunit FrdB

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)

FrdA and FrdB fumarate reductase The terminal electron-transfer enzyme fumarate reductase of Escherichia coli is a complex iron-sulfur flavoenzyme composed of four nonidentical subunits organized into two domains: FrdA and -B (a membrane-extrinsic catalytic domain) and FrdC and -D (a transmembrane anchor domain). [PMC323229] [8419359] [6751816] Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279]

[23230279] [6751816] [8419359] [PMC323229]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

Soluble IM Fraction

Cytoplasmic (Experimental)

no

yes

N

28

0.105

CYT

0

-0.200913

-

0

0

N

i

CytoplasmicMembrane

9

91

GLNK_ECOLI

P0AC55

glnK

ybaI

b0450 JW0440

cytoplasmic peripheral IM protein, Nitrogen regulatory P-II 2 GlnK

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

Encodes a member of the PII signal transduction protein family, proteins that regulate enzyme activity and gene expression in response to the intracellular nitrogen status; cytoplasmic transciption factor that binds maltotriose and ATP and shuttles between the chromosome and membranes as a peripheral protein on the the ammonium transporter AmtB.

[10231487] [11847102] [14668330] [9720863]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

Soluble IM Fraction

Cytoplasmic (Experimental)

yes

yes

N

67

0.123

CYT

0

-0.200913

-

0

0

N

i

CytoplasmicMembrane

7

41

GLRX3_ECOLI

P0AC62

grxC

yibM

b3610 JW3585

cytoplasmic peripheral IM protein, Glutaredoxin 3 GrxC

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

Disulfide bridge (12 15)

[23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasmic

yes

yes

N

30

0.112

CYT

0

-0.200913

-

0

0

N

i

Unknown

2

79

GLRX4_ECOLI

P0AC69

grxD

ydhD

b1654 JW1646

cytoplasmic peripheral IM protein, Glutaredoxin 4 GrxD

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

[15833738] [23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasm

Cytoplasmic

yes

yes

N

42

0.264

CYT

0

-0.200913

-

0

0

N

i

Unknown

2

90

GNSA_ECOLI

P0AC92

gnsA

yccL

b4517 JW0976

cytoplasmic peripheral IM protein, GnsA

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

[23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasmic

no

yes

N

50

0.102

CYT

0

-0.200913

-

0

0

N

i

Unknown

2

DNAB_ECOLI

P0ACB0

dnaB

groP grpA

b4052 JW4012

cytoplasmic nucleoid peripheral IM protein, Replicative DNA helicase DnaB

Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (Experimental)

Trigger factor-sensitive aggregation.

[23230279]

Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm

A, N, F1

N/A

Cytoplasmic

TF

yes

yes

N

3

0.105

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

9

81

HEMG_ECOLI

P0ACB4

hemG

yihB

b3850 JW3827

cytoplasmic peripheral IM protein, Protoporphyrinogen IX dehydrogenase HemG

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

[23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

Soluble IM Fraction

Cytoplasmic

yes

yes

N

33

0.11

CYT

0

-0.200913

-

0

0

N

i

Unknown

2

75

ERPA_ECOLI

P0ACC3

erpA

yadR

b0156 JW0152

cytoplasmic peripheral IM protein, Iron-sulfur cluster insertion ErpA

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

[23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasmic

yes

yes

N

49

0.128

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

9

84

GLMU_ECOLI

P0ACC7

glmU

yieA

b3730 JW3708

cytoplasmic peripheral IM protein, Bifunctional GlmU

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

[23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasm

Cytoplasmic

yes

yes

N

49

0.123

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

10

94

ISCU_ECOLI

P0ACD4

iscU

nifU yfhN

b2529 JW2513

cytoplasmic peripheral IM protein, Iron-sulfur cluster assembly scaffold IscU

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

IscS is critical for iron-sulfur (Fe-S) cluster formation and repair. IscS transfers sulfur to the coregulated "scaffold" protein IscU (NifU), on which Fe-S clusters are assembled. Disulfide bond-containing protein [25664544]

[23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasmic

yes

yes

N

20

0.105

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

9

97

MBHL_ECOLI

P0ACD8

hyaB

b0973 JW0955

cytoplasmic peripheral IM protein, Hydrogenase-1 large chain HyaB

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

Peripherar Inner Membrane Cyto side (Potential) Under anaerobic conditions Escherichia coli synthesizes three membrane-associated [NiFe]-hydrogenases (Hyd). Hyd-1 and Hyd-2 are respiratory hydrogenases with their active sites facing the periplasm. [22769583] [2180913]

[2180913] [22769583]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cell membrane; Peripheral membrane protein

Periplasmic (Experimental)

yes

yes

N

49

0.102

CYT

0

-0.200913

-

0

0

N

i

CytoplasmicMembrane

10

9

HINT_ECOLI

P0ACE7

hinT

ycfF

b1103 JW1089

cytoplasmic peripheral IM protein, Purine nucleoside phosphoramidase HinT

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

[23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasmic

yes

yes

N

57

0.182

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

9

84

DBHA_ECOLI

P0ACF0

hupA

b4000 JW3964

cytoplasmic nucleoid peripheral IM protein, DNA-binding HU-alpha HupA

Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (Experimental) Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (Potential)

Identified in nucleoids collected by sucrose gradient centrifugation and analyzed by liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS)[21541338]. Bacterial histone-like DNA-binding proteins signature (<a href="http://prosite.expasy.org/PDOC00044" targer="_blank">PDOC00044</a>).

[21541338] [23230279]

Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm

A, N, F1

N/A

Cytoplasm; nucleoid

Cytoplasmic

yes

yes

N

24

0.244

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

9

96

DBHB_ECOLI

P0ACF4

hupB

hopD

b0440 JW0430

cytoplasmic nucleoid peripheral IM protein, DNA-binding HU-beta HupB

Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (Experimental) Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (Potential)

Identified in nucleoids collected by sucrose gradient centrifugation and analyzed by liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS)[21541338]. Bacterial histone-like DNA-binding proteins signature

[21541338] [23230279]

Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm

A, N, F1

N/A

Cytoplasmic

yes

yes

N

27

0.114

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

9

HNS_ECOLI

P0ACF8

hns

bglY cur drdX hnsA msyA osmZ pilG topS

b1237 JW1225

cytoplasmic nucleoid peripheral IM protein, DNA-binding H-NS

Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (Experimental)

Experimentally verified gene-product. Methionine start codon is cleaved. Identified in nucleoids collected by sucrose gradient centrifugation and analyzed by liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS)[21541338].

[23230279]

Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm

A, N, F1

N/A

Cytoplasm; nucleoid

Cytoplasmic

yes

yes

N

19

0.173

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

10

103

STPA_ECOLI

P0ACG1

stpA

hnsB

b2669 JW2644

cytoplasmic nucleoid peripheral IM protein, DNA-binding StpA

Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (Experimental)

Identified in nucleoids collected by sucrose gradient centrifugation and analyzed by liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS)[21541338].

[21541338] [23230279]

Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm

A, N, F1

N/A

Cytoplasm; nucleoid

Cytoplasmic

yes

yes

N

19

0.184

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

9

15

LRP_ECOLI

P0ACJ0

lrp

alsB ihb livR oppI

b0889 JW0872

cytoplasmic nucleoid peripheral IM protein, Leucine-responsive regulatory protein Lrp

Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (Experimental)

[23138451] [23230279]

Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm

A, N, F1

IM (predicted)

Cytoplasmic

yes

yes

N

48

0.101

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

9

36

CRP_ECOLI

P0ACJ8

crp

cap csm

b3357 JW5702

cytoplasmic nucleoid peripheral IM protein, cAMP-activated global transcriptional regulator Crp

Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (Experimental) Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (Probable)

Trigger factor-sensitive aggregation.

[15650111] [23138451] [23230279]

Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm

A, N, F1

N/A

Cytoplasmic

TF

yes

yes

N

24

0.105

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

9

YFIA_ECOLI

P0AD49

raiA

yfiA

b2597 JW2578

cytoplasmic peripheral IM protein, Ribosome-associated inhibitor A RaiA

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

Translation

[23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

Soluble IM Fraction

Cytoplasmic

yes

yes

N

63

0.108

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

10

98

KPYK1_ECOLI

P0AD61

pykF

b1676 JW1666

cytoplasmic peripheral IM protein, Pyruvate kinase I PykF

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

[23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasmic

yes

yes

N

42

0.167

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

9

108

AMPH_ECOLI

P0AD70

ampH

yaiH

b0376 JW5052

cytoplasmic peripheral IM protein (SEC through IM), D-alanyl-D-alanine-carboxypeptidase/endopeptidase AmpH

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)

Potential substrate of periplasmic peptidyl prolyl cis???trans isomerase SurA. Sequence contains known binding motif of SurA [RYEF]X[RYEF][P]. The N-terminal export signal of AmpH is processed, but the protein remains membrane associated. Disulfide bond-containing protein [25664544]

[22001512]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cell inner membrane

Periplasmic

SURA

yes

yes

Y

22

0.613

SpI

21

16.801

-

0

0

Y

n5-16c21/22o

Periplasmic

9

41

YGAU_ECOLI

P0ADE6

ygaU

yzzM

b2665 JW2640

cytoplasmic peripheral IM protein, Potassium binding Kbp YgaU

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Potential) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)

Contains Peptidoglycan-binding LysM and phospholipid binding Bon domain but no signal peptide. Identified in nucleoids collected by sucrose gradient centrifugation and analyzed by liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS). [21541338] Contains Peptidoglycan-binding LysM and phospholipid binding Bon domain but no signal peptide. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279] The OsmY protein is an Escherichia coli 20 kDa outer membrane or periplasmic protein that is expressed in response to a variety of stress conditions, in particular, helping to provide protection against osmotic shock. One hypothesis is that OsmY prevents shrinkage of the cytoplasmic compartment by contacting the phospholipid interfaces surrounding the periplasmic space. The domain architecture of two BON domains alone suggests that these domains contact the surfaces of phospholipids, with each domain contacting a membrane [12175925]. Homologous to OsmY.

[12878000] [21541338] [23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

Soluble IM Fraction

Cytoplasmic

yes

yes

N

10

0.101

CYT

0

-0.200913

-

0

0

N

i

Unknown

2

89

SUHB_ECOLI

P0ADG4

suhB

ssyA

b2533 JW2517

cytoplasmic peripheral IM protein, Inositol-1-monophosphatase SuhB

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

SuhB forms a tight complex with RNA polymerase (RNA pol) that inhibits the IMPase catalytic activity of SuhB

[23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasmic

yes

yes

N

21

0.179

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

9

86

IMDH_ECOLI

P0ADG7

guaB

guaR

b2508 JW5401

cytoplasmic peripheral IM protein, Inosine-5'-monophosphate dehydrogenase GuaB

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

Prediction of one very weak transmembrane helix.

[15919657] [23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

IM (predicted)

Cytoplasmic

yes

yes

N

29

0.14

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

9

49

ENTB_ECOLI

P0ADI4

entB

entG

b0595 JW0587

cytoplasmic peripheral IM protein, Enterobactin synthase component B EntB

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

[10692387]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasm

Cytoplasmic

yes

yes

N

26

0.118

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

8

65

YIEE_ECOLI

P0ADM8

yieE

b3712 JW3690

cytoplasmic peripheral IM protein, YieE

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Potential)

No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius.

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

IM (predicted-detected)

Cytoplasmic

yes

yes

N

25

0.109

CYT

0

-0.200913

-

0

0

N

i

Unknown

2

7

YIFE_ECOLI

P0ADN2

yifE

b3764 JW3737

cytoplasmic peripheral IM protein, UPF0438 YifE

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

[23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasmic

yes

yes

N

44

0.105

CYT

0

-0.200913

-

0

0

N

i

Unknown

2

YIGI_ECOLI

P0ADP2

yigI

b3820 JW5588

cytoplasmic peripheral IM protein, YigI

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Potential) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)

No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279]

[19766000] [23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

IM (predicted-detected), Soluble IM Fraction +

Cytoplasmic

yes

yes

N

28

0.137

CYT

0

-0.200913

-

0

0

N

i

Unknown

2

9

ZAPA_ECOLI

P0ADS2

zapA

ygfE

b2910 JW2878

cytoplasmic peripheral IM protein, Cell division ZapA

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

Binds to the FtsZ ring. ZapA interacts directly with itself, FtsN, FtsZ and FtsI. Peripheral inner membrane protein via binding to the FtsZ ring. ZapA orthologue, YgfE, binds FtsZ polymers and promotes FtsZ polymer bundling. [12368265]

[12368265] [17428494] [20487275] [20497333]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasm

Cytoplasmic

yes

yes

N

67

0.152

CYT

0

-0.200913

-

0

0

N

i

Unknown

2

97

YGIB_ECOLI

P0ADT2

ygiB

b3037 JW5927

cytoplasmic peripheral IM protein, UPF0441 YgiB

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

YgiB and ygiC genes are expressed in the same operon as tolC and have homologues (yjfM and yjfC) located elsewhere on the chromosome. Mutational inactivation of the outer membrane channelTolC (a key component of multidrug efflux and type I secretion transporters) renders cells highly susceptible to antibiotics and leads to defects in secretion of protein toxins and the loss of TolC impairs cell division and growth in minimal glucose medium due to metabolic shutdown. The TolC-dependent phenotype was further exacerbated by the loss of ygiB and ygiC genes. YgiBC (and YjfMC) may have parallel but independent functions from TolC. Disulfide bond-containing protein [25664544]

[20545840] [23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

Soluble IM Fraction

Cytoplasmic

yes

yes

N

36

0.178

SpII

36

9.72422

Pos+2=E

0

0

Y

n21-32c37/38o

Periplasmic

9

TRMJ_ECOLI

P0AE01

trmJ

yfhQ

b2532 JW2516

cytoplasmic peripheral IM protein, tRNA (cytidine/uridine-2'-O-)-methyltransferase TrmJ

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

[23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasm

Cytoplasmic

yes

yes

N

63

0.12

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

9

78

AHPC_ECOLI

P0AE08

ahpC

b0605 JW0598

cytoplasmic peripheral IM protein, Alkyl hydroperoxide reductase C AhpC

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

Disulfide bridge (47 47;166 166) Disulfide bond-containing protein [25664544]

[23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasm

Cytoplasmic

yes

yes

N

49

0.111

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

10

90

MAP1_ECOLI

P0AE18

map

b0168 JW0163

cytoplasmic peripheral IM protein, Methionine aminopeptidase Map

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

Has been viewed through fluorescence microscopy and located evenly throughout cytoplasm. Small scale study of labeling chromosomal coding DNA sequences (CDSs) with fluorescent protein genes. [17272300]

[17272300] [23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasmic

yes

yes

N

22

0.108

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

9

63

CPXR_ECOLI

P0AE88

cpxR

yiiA

b3912 JW3883

cytoplasmic nucleoid peripheral IM protein, transcriptional regulator CpxR

Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (Experimental)

[23138451] [23230279] [8294007]

Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm

A, N, F1

N/A

Cytoplasm

Cytoplasmic

yes

yes

N

32

0.14

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

10

118

USPA_ECOLI

P0AED0

uspA

b3495 JW3462

cytoplasmic peripheral IM protein, Universal stress A UspA

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (By similarity) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)

The Usp superfamily encompasses an ancient and conserved group of proteins that are found in bacteria, archaea,fungi, flies and plants. Usually each organism has several copies of Usp genes. Escherichia coli contains six Usp proteins, which can be divided into two subfamilies on the basis of sequence similarity. [19101563] Three of these proteins belong to the UspA subfamily (UspA, UspC, and UspD), and two to the UspFG subfamily (UspF and UspG). Another homologue is YbdQ. UspC binds to the membrane-integrated sensor kinase KdpD involved in K+ ion sensing. The same could be true for the other Usp proteins but we could not find direct evidence in the literature. [1453957] [8506346] [8740179] [9405142] Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279]

[1453957] [15911532] [19101563] [23230279] [8506346] [8740179] [9405142]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

Soluble IM Fraction

Cytoplasm

Cytoplasmic (Experimental)

yes

yes

N

33

0.153

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

10

80

FABG_ECOLI

P0AEK2

fabG

b1093 JW1079

cytoplasmic peripheral IM protein, 3-oxoacyl-(acyl-carrier-protein) reductase FabG

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

[23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasmic

yes

yes

N

23

0.249

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

9

93

FABI_ECOLI

P0AEK4

fabI

envM

b1288 JW1281

cytoplasmic peripheral IM protein, Enoyl-(acyl-carrier-protein) reductase (NADH) FabI

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

Trigger factor-sensitive aggregation. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279]

[23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasmic

TF

yes

yes

N

28

0.245

CYT

0

-0.200913

-

0

0

N

i

CytoplasmicMembrane

10

48

GYRA_ECOLI

P0AES4

gyrA

hisW nalA parD

b2231 JW2225

cytoplasmic nucleoid peripheral IM protein, DNA gyrase subunit A GyrA

Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (Experimental)

Trigger factor-sensitive aggregation.

[23230279]

Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm

A, N, F1

N/A

Cytoplasm

Cytoplasmic

TF

yes

yes

N

36

0.106

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

9

49

GYRB_ECOLI

P0AES6

gyrB

acrB cou himB hisU nalC parA pcbA

b3699 JW5625

cytoplasmic nucleoid peripheral IM protein, DNA gyrase subunit B GyrB

Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (Experimental)

Trigger factor-sensitive aggregation.

[23230279]

Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm

A, N, F1

N/A

Cytoplasm

Cytoplasmic

TF

yes

yes

N

54

0.109

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

9

MIND_ECOLI

P0AEZ3

minD

b1175 JW1164

cytoplasmic peripheral IM protein, Septum site-determining MinD

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

MinD recruits MinC to the membrane to form the MinC-MinD complex, which inhibits septum formation, and MinE acts to localize and restrict inhibition to the inappropriate septation sites. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279]

[10096083] [23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cell inner membrane; Peripheral membrane protein

Cytoplasmic

yes

yes

N

25

0.128

CYT

0

-0.200913

-

0

0

N

i

CytoplasmicMembrane

10

95

MOAB_ECOLI

P0AEZ9

moaB

chlA2

b0782 JW0765

cytoplasmic peripheral IM protein, Molybdenum cofactor biosynthesis B MoaB

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Potential) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)

Experimentally detected by proteomics analysis of E.coli membrane fraction (Bernsel et al 2009). No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Potential peripherally associated protein facing the cytoplasm or integral inner membrane protein. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279]

[19766000] [23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

Soluble IM Fraction

Cytoplasmic

yes

yes

N

24

0.114

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

9

105

ZAPB_ECOLI

P0AF36

zapB

yiiU

b3928 JW3899

cytoplasmic peripheral IM protein, Cell division ZapB

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

[18394147] [20487275]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasm

Cytoplasmic

no

yes

N

19

0.122

CYT

0

-0.200913

-

0

0

N

i

Unknown

2

81

RRAB_ECOLI

P0AF90

rraB

yjgD

b4255 JW4212

cytoplasmic nucleoid peripheral IM protein, Regulator of ribonuclease activity B RraB

Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (Experimental)

Negative regulator of RNaseE. Part of membrane-associating assemblies that coil around the periphery of the cell when visualized by immunofluorescence microscopy. These assemblies co-localize with the RNA degradosome and participate in the RNA degradation process. [24438330]

[24438330]

Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm

A, N, F1

N/A

Cytoplasm

Cytoplasmic

yes

yes

N

41

0.107

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

8

RIDA_ECOLI

P0AF93

ridA

yjgF

b4243 JW5755

cytoplasmic peripheral IM protein, 2-iminobutanoate/2-iminopropanoate deaminase RidA

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

Experimentally verified gene-product. Methionine start codon is cleaved. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279]

[23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasm

Cytoplasmic

yes

yes

N

21

0.115

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

9

75

NUOB_ECOLI

P0AFC7

nuoB

b2287 JW5875

cytoplasmic peripheral IM protein, NADH-quinone oxidoreductase subunit B NuoB

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

Crystal structure of the entire respiratory complex I [23417064] Crystal structure of the entire respiratory complex I [21822288] [7607227] Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279]

[15037611] [19189973] [21822288] [23230279] [23417064] [7607227]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

IM (predicted)

Cell inner membrane; Peripheral membrane protein; Cytoplasmic side

Cytoplasmic (Experimental)

yes

yes

N

62

0.1

CYT

0

-0.200913

-

0

0

N

i

CytoplasmicMembrane

9

78

NUOE_ECOLI

P0AFD1

nuoE

b2285 JW2280

cytoplasmic peripheral IM protein, NADH-quinone oxidoreductase subunit E NuoE

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)

NADH dehydrogenase I is composed of 13 different subunits. Subunits nuoCD, E, F, and G constitute the peripheral sector of the complex[19737520]. Thermus homologue: Q56221 (NQO2_THET8). Copurifies with trigger factor in pull down assays[19737520]. [11416161] [7607227] [7690854] Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279]

[11416161] [23230279] [23543743] [7607227] [7690854]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

Soluble IM Fraction

Cytoplasmic (Experimental)

TF

yes

yes

N

67

0.103

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

9

31

NUOI_ECOLI

P0AFD6

nuoI

b2281 JW2276

cytoplasmic peripheral IM protein, NADH-quinone oxidoreductase subunit I NuoI

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (By similarity) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

[11416161] [15037611] [19189973] [21822288] [23230279] [23417064]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

Soluble IM Fraction

Cell inner membrane

Cytoplasmic (Experimental)

yes

yes

N

27

0.17

CYT

0

-0.200913

-

0

1

N

o6-26i

Cytoplasmic

9

72

NUSA_ECOLI

P0AFF6

nusA

b3169 JW3138

cytoplasmic nucleoid peripheral IM protein, Transcription termination/antitermination NusA

Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (Experimental)

Fluorescent fusions of NusA have been monitored via fluorescence microscopy. NusA was found to be located predominantly in two clusters associated with each nucleoid. NusA can self-assemble into oligomers under heat shock conditions. NusA also acquires chaperone activity.

[17272300] [23230279]

Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm

A, N, F1

N/A

Cytoplasm

Cytoplasmic

yes

yes

N

41

0.104

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

9

99

NUSG_ECOLI

P0AFG0

nusG

b3982 JW3945

cytoplasmic peripheral IM protein, Transcription termination/antitermination NusG

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

[23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasmic

yes

yes

N

26

0.108

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

9

72

ODO1_ECOLI

P0AFG3

sucA

b0726 JW0715

cytoplasmic peripheral IM protein, 2-oxoglutarate dehydrogenase E1 component SucA

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

Trigger factor-sensitive aggregation.

[15919657] [23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

Soluble IM Fraction

Cytoplasmic

TF

yes

yes

N

24

0.133

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

9

6

ODO2_ECOLI

P0AFG6

sucB

b0727 JW0716

cytoplasmic peripheral IM protein, Dihydroyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex SucB

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

Trigger factor-sensitive aggregation. Copurifies with trigger factor in pull down assays. [9298646]

[15919657] [23230279] [9298646]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

Soluble IM Fraction

Cytoplasmic (Experimental)

TF

yes

yes

N

19

0.112

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

9

122

ODP1_ECOLI

P0AFG8

aceE

b0114 JW0110

cytoplasmic peripheral IM protein, Pyruvate dehydrogenase E1 component AceE

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (By similarity) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

AceE is the E1 subunit of the 10MDa Escherichia coli pyruvate dehydrogenase multienzyme complex, which catalyzes the oxidative decarboxylation of the central metabolite pyruvate to CO(2) and acetate (crystal structure solved as a dimer [11955070]). The E1 subunit is present as a 24mer within the complex. AceE is also part of a very large complex partially resolved by cryoEM [16338405]. Analogous enzyme pyryvate oxidase-PoxB is membrane associated [18988747]. Trigger factor-sensitive aggregation. Outer membrane protein determined by LC-MS (Gevaert et al 2002) Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279]

[15919657] [18988747] [23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

IM (predicted)

Cytoplasmic

TF

yes

yes

N

53

0.105

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

9

15

PARC_ECOLI

P0AFI2

parC

b3019 JW2987

cytoplasmic nucleoid peripheral IM protein, DNA topoisomerase 4 subunit A ParC

Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (Experimental)

Dimer of topoisomerase IV subunit A <a href="http://BioCyc.org/ECOLI/NEW-IMAGE?type=ENZYME&object=EG10686-MONOMER">(bioCyc)</a> Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279]

[1334483] [23230279]

Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm

A, N, F1

N/A

Cell membrane; Peripheral membrane protein

Cytoplasmic

yes

yes

N

18

0.118

CYT

0

-0.200913

-

0

0

N

i

CytoplasmicMembrane

10

17

PDXH_ECOLI

P0AFI7

pdxH

b1638 JW1630

cytoplasmic peripheral IM protein, Pyridoxine/pyridoxamine 5'-phosphate oxidase PdxH

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

Copurifies with trigger factor in pull down assays.

[23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasmic

TF

yes

yes

N

43

0.121

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

9

51

PHOB_ECOLI

P0AFJ5

phoB

b0399 JW0389

cytoplasmic nucleoid peripheral IM protein, Phosphate regulon transcriptional regulatory PhoB

Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (Experimental)

PhoB is a dual transcription regulator that activates expression of the Pho regulon in response to environmental Pi. Under phosphate limitation conditions the inner membrane sensor kinase PhoR autophosphorylates; Subsequent transfer of the phosphate group to PhoB results in activation of PhoB. It has been shown that PhoBR and PhoB are responsible for upregulation of phosphonate and glycerol phosphate metabolism and the high-affinity phosphate transport system, respectively, thus showing the complex regulation by the PhoR-PhoB two-component regulatory system.

[18031348] [23138451] [2693738]

Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm

A, N, F1

N/A

Cytoplasm

Cytoplasmic

yes

yes

N

33

0.201

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

9

PPX_ECOLI

P0AFL6

ppx

b2502 JW2487

cytoplasmic peripheral IM protein, Exopolyphosphatase Ppx

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279]

[23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

IM (predicted)

Cell membrane

Cytoplasmic

yes

yes

N

29

0.112

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

10

47

PSPA_ECOLI

P0AFM6

pspA

b1304 JW1297

cytoplasmic peripheral IM protein, Phage shock A PspA

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)

Forms large oligomers and binds directly to phospholipids (preferentially phosphatidylserine and phosphatidylglycerol). PMC3709507 Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279]

[19555453] [23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasm

Cytoplasmic

Prophage Transposase

yes

no

N

23

0.112

CYT

0

-0.200913

-

0

0

N

i

CytoplasmicMembrane

10

113

YBBO_ECOLI

P0AFP4

ybbO

b0493 JW0482

cytoplasmic nucleoid peripheral IM protein, Oxidoreductase YbbO

Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (Experimental)

[23138451] [23230279]

Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm

A, N, F1

Soluble IM Fraction

Cytoplasmic

no

yes

N

45

0.12

CYT

0

-0.200913

-

0

1

N

o240-258i

Cytoplasmic

9

PSPD_ECOLI

P0AFV8

pspD

b1307 JW1300

cytoplasmic peripheral IM protein, Phage shock D PspD

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)

Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279]

[12562786] [23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasm

Cytoplasmic

Prophage Transposase

no

no

N

64

0.182

CYT

0

-0.200913

-

0

0

N

i

Unknown

2

34

SEQA_ECOLI

P0AFY8

seqA

b0687 JW0674

cytoplasmic peripheral IM protein, Negative modulator of initiation of replication SeqA

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

Trigger factor-sensitive aggregation. Membrane-associated oriC-binding machinery of E. coli is a multiprotein complex that includes the SeqA and SeqB proteins. [9660922] Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279]

[23230279] [9660922] [9736699]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasm

Cytoplasmic

TF

yes

yes

N

43

0.12

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

8

13

SSPB_ECOLI

P0AFZ3

sspB

b3228 JW3197

cytoplasmic peripheral IM protein, Stringent starvation B SspB

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

[23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasmic

yes

yes

N

47

0.112

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

8

PUR1_ECOLI

P0AG16

purF

b2312 JW2309

cytoplasmic peripheral IM protein, Amidophosphoribosyltransferase PurF

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)

Experimentally detected by proteomics analysis of E.coli membrane fraction (Bernsel et al 2009). No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Potential peripherally associated protein facing the cytoplasm or integral inner membrane protein.

[19766000]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

Soluble IM Fraction

Cytoplasmic

yes

yes

N

32

0.119

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

9

15

RELA_ECOLI

P0AG20

relA

b2784 JW2755

cytoplasmic peripheral IM protein, GTP pyrophosphokinase RelA

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

[23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasmic

no

yes

N

37

0.119

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

8

75

RHO_ECOLI

P0AG30

rho

nitA psuA rnsC sbaA tsu

b3783 JW3756

cytoplasmic peripheral IM protein, Transcription termination factor Rho

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

[23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasmic

yes

yes

N

44

0.103

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

9

SECB_ECOLI

P0AG86

secB

b3609 JW3584

cytoplasmic peripheral IM protein, Protein-export SecB

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

Cytoplasmic protein that forms transient interactions with the membrane; binds to the C-terminus of SecA. Protein that acts as its membrane receptor. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279]

[2170023] [23230279] [9767586] [PMC1326294]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasm

Cytoplasmic

yes

yes

N

53

0.133

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

10

88

RPOE_ECOLI

P0AGB6

rpoE

sigE

b2573 JW2557

cytoplasmic nucleoid peripheral IM protein, ECF RNA polymerase sigma-E factor RpoE

Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (By similarity) Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (Experimental) Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (Probable)

SigmaE controls the ??E-dependent extracytoplasmic stress response and is required to maintain cell envelope integrity in Escherichia coli [9159523] [18253509]. RseA, encodes an anti-sigma(E) activity demonstrated at both the genetic and biochemical levels [9159522]. RseC is an inner membrane protein that positively modulates sigma(E) activity [9159522]. ??E is held at the membrane by the antisigma factor RseA. Proteolysis of transmembrane and cytoplasmic portions of RseA by DegS frees the sigma(E) transcription factor. RseB binds to the periplasmic domain of RseA and protects RseA from proteolysis. [17360428]. Unfolded OMPs activate the protease DegS, which cleave RpoE regulon [15520285]. Identified in the inner membrane by biochemical followed by two-dimensional gel electrophoresis and tandem mass spectrometry [15911532]. [15774872] [16597971] [11777003] Crystal structure of Escherichia coli sigmaE with the cytoplasmic domain of its anti-sigma RseA reveals that RseA functions by sterically occluding the two primary binding determinants on sigma(E) for core RNA polymerase. [12718891] Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279]

[11777003] [15520285] [15774872] [15911532] [16597971] [17360428] [20971899] [23138451] [23230279] [7889934] [9159522]

Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm

A, N, F1

Soluble IM Fraction

Cytoplasm

Cytoplasmic (Experimental)

yes

yes

N

44

0.111

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

10

101

SRP54_ECOLI

P0AGD7

ffh

b2610 JW5414

cytoplasmic peripheral IM protein, Signal recognition particle Ffh

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

Trigger factor-sensitive aggregation. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279]

[18281057] [23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasm

Cytoplasmic

TF

yes

yes

N

63

0.1

CYT

0

-0.200913

-

0

0

N

i

Unknown

5

106

SUCD_ECOLI

P0AGE9

sucD

b0729 JW0718

cytoplasmic peripheral IM protein, Succinate--CoA ligase (ADP-forming) subunit alpha SucD

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

Trigger factor-sensitive aggregation.

[23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasmic

TF

yes

yes

N

31

0.171

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

9

70

TDCB_ECOLI

P0AGF6

tdcB

b3117 JW3088

cytoplasmic peripheral IM protein, L-threonine dehydratase catabolic TdcB

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Potential)

No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Potential substrate of periplasmic chaperone YfgM. [25403562] Putative substrate for the periplasmic chaperone YfgM. [25403562]

[25403562]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

IM (predicted-detected)

Cytoplasmic

YfgM

yes

yes

N

23

0.152

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

9

72

TRKA_ECOLI

P0AGI8

trkA

b3290 JW3251

cytoplasmic peripheral IM protein, Trk system potassium uptake TrkA

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)

[23230279] [2674131]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

Soluble IM Fraction

Cell inner membrane

Cytoplasmic

no

yes

N

17

0.13

CYT

0

-0.200913

-

0

0

Y

n3-11c16/17o

CytoplasmicMembrane

10

68

YFIF_ECOLI

P0AGJ5

yfiF

b2581 JW2565

cytoplasmic peripheral IM protein, tRNA/rRNA methyltransferase YfiF

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

Copurifies with trigger factor in pull down assays.

[23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasmic

TF

no

yes

N

45

0.103

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

9

81

SYY_ECOLI

P0AGJ9

tyrS

b1637 JW1629

cytoplasmic peripheral IM protein, Tyrosine--tRNA ligase TyrS

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

Translation unit

[23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasm

Cytoplasmic

no

yes

N

65

0.123

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

10

63

RSME_ECOLI

P0AGL7

rsmE

yggJ

b2946 JW2913

cytoplasmic peripheral IM protein, Ribosomal RNA small subunit methyltransferase E RsmE

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

Ribosomal

[23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

IM (predicted)

Cytoplasm

Cytoplasmic

yes

yes

N

40

0.129

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

8

18

RLME_ECOLI

P0C0R7

rlmE

ftsJ mrsF rrmJ

b3179 JW3146

cytoplasmic peripheral IM protein, Ribosomal RNA large subunit methyltransferase E RlmE

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

Assembly cofactor of ribosome. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279]

[21529161] [23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasm

Cytoplasmic

yes

yes

N

29

0.111

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

9

16

GATY_ECOLI

P0C8J6

gatY

yegF

b2096 JW5343

cytoplasmic peripheral IM protein, D-tagatose-1,6-bisphosphate aldolase subunit GatY

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)

Trigger factor-sensitive aggregation. Experimentally detected by proteomics analysis of E.coli membrane fraction (Bernsel et al 2009). No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Potential peripherally associated protein facing the cytoplasm or integral inner membrane protein.

[19766000]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

Soluble IM Fraction

Cytoplasmic

TF

yes

yes

N

49

0.137

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

9

11

GATZ_ECOLI

P0C8J8

gatZ

b2095 JW2082

cytoplasmic peripheral IM protein, D-tagatose-1,6-bisphosphate aldolase subunit GatZ

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

Encoded in the phosphotransferase transport system the hexitol galactitol (Gat), binds to the gatY protein that encodes for a D-tagatose-1,6-bisphosphate aldolase. Trigger factor-sensitive aggregation. Trigger factor-sensitive aggregation. Copurifies with trigger factor in pull down assays. Disulfide bond-containing protein [25664544]

[23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

Soluble IM Fraction

Cytoplasmic

TF

yes

yes

N

33

0.318

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

8

6

EFTU1_ECOLI

P0CE47

tufA

b3339 JW3301

cytoplasmic peripheral IM protein, Elongation factor Tu 1 TufA

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (By similarity) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)

Translation initiation factor EF-Tu. Ribosome association and peripheral inner membrane protein. In B. subtilis EF-Tu localizes in a helical pattern underneath the cell membrane and colocalizes with MreB, an actin-like cytoskeletal. [7525272] [7021545] Interacts with DmsD is a redox enzyme maturation protein (REMP). [20153451] Disulfide bond-containing protein [25664544]

[20133608] [20153451] [2022614] [7021545] [7525272] [775340]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasm Cell inner membrane; Peripheral membrane protein

Cytoplasmic (Experimental)

yes

yes

N

47

0.197

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

10

99

EFTU2_ECOLI

P0CE48

tufB

b3980 JW3943

cytoplasmic peripheral IM protein, Elongation factor Tu 2 TufB

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)

[20153451] [2022614] [23230279] [775340]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

Soluble IM Fraction

Cytoplasm Cell inner membrane; Peripheral membrane protein

Cytoplasmic

yes

yes

N

47

0.197

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

10

87

RCSB_ECOLI

P0DMC7

rcsB

b2217 JW2205

cytoplasmic nucleoid peripheral IM protein, transcriptional regulator RcsB

Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (Experimental) Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (Potential)

Regulator protein RcsB is a member of the two-component regulatory system RcsC/RcsB. It is involved in the activation of colanic acid capsule synthesis (cps) and cell division (ftsZ) genes. It is believed that the RcsB protein is activated through phosphorylation, probably by the sensor protein RcsC. RcsB protein for Regulator capsule synthesis B, is a response regulator that belongs to the multicomponent RcsF/RcsC/RcsD/RcsA-RcsB phosphorelay system. LuxR-type HTH domain signature and profile. The luxR-type HTH domain is a DNA-binding, helix-turn-helix (HTH) domain of about 65 amino acids, present in transcription regulators of the LuxR/FixJ family of response regulators (PROSITE: PDOC0054).

[2404948]

Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm

A, N, F1

N/A

Cytoplasmic

no

yes

N

66

0.117

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

10

90

FTSY_ECOLI

P10121

ftsY

b3464 JW3429

cytoplasmic peripheral IM protein, Signal recognition particle receptor FtsY

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)

Contains an essential and autonomous membrane-binding amphipathic helix. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279]

[11353766] [17726013] [18281057] [19414018] [21543314] [23230279] [8194520] [9177162]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cell inner membrane

Cytoplasmic

yes

yes

N

3

0.107

CYT

0

-0.200913

-

0

0

N

i

CytoplasmicMembrane

10

GLNQ_ECOLI

P10346

glnQ

b0809 JW0794

cytoplasmic peripheral IM protein, Glutamine transport ATP-binding GlnQ

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)

Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279]

[16079137] [23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

Soluble IM Fraction

Cell inner membrane

Cytoplasmic (Experimental)

yes

yes

N

42

0.105

CYT

0

-0.200913

-

0

0

N

i

CytoplasmicMembrane

10

97

ENTE_ECOLI

P10378

entE

b0594 JW0586

cytoplasmic peripheral IM protein, Enterobactin synthase component E EntE

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

Homologous to FadD13 protein of Mycobacterium that is peripheral. Phobius predits transmembrane region around aa100. Binds AMP. Very high homology to eight other transport proteins: (FADK_ECOLI, CAIC_ECOLI, LCFA_ECOLI, ENTF_ECOLI, ACSA_ECOLI) Membrane Association of the Escherichia coli Enterobactin Synthase Proteins EntB/G, EntE, and EntF

[10692387]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Membrane

Cytoplasmic

yes

yes

N

36

0.15

CYT

0

-0.200913

-

0

1

N

o86-105i

Cytoplasmic

9

88

SECA_ECOLI

P10408

secA

azi pea prlD

b0098 JW0096

cytoplasmic peripheral IM protein, translocase subunit SecA

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279]

[15919657] [23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

Soluble IM Fraction

Cell inner membrane; Peripheral membrane protein; Cytoplasmic side Cytoplasm

Cytoplasmic

no

yes

N

70

0.109

CYT

0

-0.200913

-

0

0

N

i

CytoplasmicMembrane

10

58

LPXB_ECOLI

P10441

lpxB

pgsB

b0182 JW0177

cytoplasmic peripheral IM protein, Lipid-A-disaccharide synthase LpxB

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)

It has been demonstrate by mass spectrometry and autoradiography that 1.6 to 3.5 phospholipid molecules co-purify with each LpxB monomer[19883124]. It has been demonstrate by mass spectrometry and autoradiography that 1.6 to 3.5 phospholipid molecules co-purify with each LpxB monomer. [19883124] Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279]

[19883124] [23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

Soluble IM Fraction

Cytoplasmic

yes

yes

N

23

0.146

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

10

47

UGPC_ECOLI

P10907

ugpC

b3450 JW3415

cytoplasmic peripheral IM protein, sn-glycerol-3-phosphate import ATP-binding UgpC

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279]

[23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cell inner membrane; Peripheral membrane protein

Cytoplasmic

yes

yes

N

37

0.105

CYT

0

-0.200913

-

0

0

N

i

CytoplasmicMembrane

10

20

LIT_ECOLI

P11072

lit

b1139 JW1125

cytoplasmic peripheral IM protein, Cell death peptidase Lit

Single weak hydrophobic region. Could be a transmembrane domain but could also be a peripheral inner membrane protein.

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cell membrane

Cytoplasmic

Prophage Integrase (EcoGene)

no

no

N

20

0.181

CYT

0

-0.200913

-

0

1

N

i61-81o

Cytoplasmic

8

NARH_ECOLI

P11349

narH

b1225 JW1216

cytoplasmic peripheral IM protein, Respiratory nitrate reductase 1 beta chain NarH

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)

Part of the nitrate reductase A (NarGHI) of Escherichia coli which is membrane anchored through the NarI subunit. NarH is the electron transfer subunit. [12910261] In vivo associations of Escherichia coli NarJ with a peptide of the first 50 residues of nitrate reductase catalytic subunit NarG. [19295650] When E. coli is grown anaerobically in the presence of high levels of nitrate, the respiratory chain that is formed is terminated by NarGHI otherwise known as the quinol nitrate oxidoreductase. [2674654] The heterotrimeric NarGHI has an ?????? structure encoded by narGHI respectively. The ?? and ?? subunits can be purified as a soluble NarGH dimer in the cytoplasm showing that these 2 subunits associate to form a stable subcomplex before association with the membrane ?? subunit. [3371362] The NarGH subcomplex is anchored to the cytoplasmic side of the membrane by NarI. NarJ interacts with apoNarGH early on, promoting the insertion of the [4Fe???S] clusters and molybdenum in a sequential manner. Insertion of the [Fe???S] clusters in NarH are not dependent on NarJ. Binding of NarJ also prevents premature targeting of the NarGH dimer to the membrane. [16286471] The NarGH subcomplex is anchored to the cytoplasmic side of the membrane by NarI. NarJ interacts with apoNarGH early on, promoting the insertion of the [4Fe???S] clusters and molybdenum in a sequential manner. Insertion of the [Fe???S] clusters in NarH are not dependent on NarJ. Binding of NarJ also prevents premature targeting of the NarGH dimer to the membrane. [16286471] [3053688] [2233673] [2674654] Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279]

[12910261] [2233673] [23230279] [3053688]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

Soluble IM Fraction

Cell membrane; Peripheral membrane protein

Cytoplasmic (Experimental)

yes

yes

N

32

0.117

CYT

0

-0.200913

-

0

0

N

i

CytoplasmicMembrane

10

13

ARLY_ECOLI

P11447

argH

b3960 JW3932

cytoplasmic peripheral IM protein, Argininosuccinate lyase ArgH

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

[23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasm

Cytoplasmic

yes

yes

N

53

0.138

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

9

ENTF_ECOLI

P11454

entF

b0586 JW0578

cytoplasmic peripheral IM protein, Enterobactin synthase component F EntF

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

[10692387]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasmic

yes

yes

N

56

0.235

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

9

20

BGLG_ECOLI

P11989

bglG

bglC

b3723 JW3701

cytoplasmic peripheral IM protein, Cryptic beta-glucoside bgl operon antiterminator BglG

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

BglG is a transcriptional antiterminator which controls the expression bgl operon. It has been detected at the membrane when it is expressed with BglF, when expressed as monocistron, the bglG transcripts were observed at the cell poles (unpublished data). In the absence of ??-glucosidase, BglG also assembles near membrane and forms a pro-form complex with BglF that maintains BglG inactive. In the presence of sugar, BglG associates with the proteins of the sugar phosphotransfer system (PTS) and localizes at the cell poles. BglG requires two distinct (de)phosphorylation events to gain activity.

[22984181]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasmic

no

yes

N

38

0.103

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

8

48

METH_ECOLI

P13009

metH

b4019 JW3979

cytoplasmic peripheral IM protein, Methionine synthase MetH

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

[23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasmic

yes

yes

N

35

0.124

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

9

GLPB_ECOLI

P13033

glpB

b2242 JW2236

cytoplasmic peripheral IM protein, Anaerobic glycerol-3-phosphate dehydrogenase subunit B GlpB

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (By similarity) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

Confirmed cytoplasmic membrane localization of GlpB. GlpB has been isolated from inner membrane preparations. Part of anaerobic glycerol-3-phosphate dehydrogenase composed of a catalytic GlpA/B dimer and of membrane bound GlpC. GlpD enzyme is membrane bound. The active site has been located at the cytoplasmic (inner) face of the membrane. [6387427] Glycerol-P dehydrogenase is a primary dehydrogenase in the electron transport chain. It functions in vitro only when associated with the cytoplasmic membrane. The enzyme has been purified from the membrane fraction after solubilization with deoxycholate. [1987111] [PMC3210124] [3286606]

[1987111] [20706981] [3286606] [6387427] [PMC3210124]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

IM (predicted)

Cell inner membrane; Peripheral membrane protein

Cytoplasmic (Experimental)

yes

yes

N

30

0.137

CYT

0

-0.200913

-

0

0

Y

n10-21c28/29o

CytoplasmicMembrane

10

24

GLPD_ECOLI

P13035

glpD

glyD

b3426 JW3389

cytoplasmic peripheral IM protein, Aerobic glycerol-3-phosphate dehydrogenase GlpD

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)

Binds to the cytoplasmic membrane through an amphipathic C-terminal alpha-helix (357-374)[11955283]. Identified in PlsB-PflB-GlpD-Psd [20521846]. Detergent-depleted enzyme can be functionally reconstituted with Escherichia coli membrane vesicles to support glycerol-3-phosphate-dependent active transport of L-proline[340460]. When GlpD is lost by mutation, the cells not only fail to grow on glycerol or l-alpha-glycerophosphate, but are subject to growth inhibition in the presence of either compound [5321485]. Glycerol-P dehydrogenase is a primary dehydrogenase in the electron transport chain. It functions in vitro only when associated with the cytoplasmic membrane. The enzyme has been purified from the membrane fraction after solubilization with deoxycholate. [1987111] GlpD enzyme is membrane bound. The active site has been located at the cytoplasmic (inner) face of the membrane. [6387427] Identified in the inner membrane by biochemical fractionation followed by two-dimensional gel electrophoresis and tandem mass spectrometry. [15911532] [8982270] Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279]

[11955283] [15911532] [15919657] [23230279] [340460] [8982270]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

Soluble IM Fraction

Cytoplasm

Cytoplasmic (Experimental)

yes

yes

N

35

0.138

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

10

48

RPOS_ECOLI

P13445

rpoS

appR katF nur otsX sigS

b2741 JW5437

cytoplasmic nucleoid peripheral IM protein, RNA polymerase sigma factor RpoS

Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (Experimental)

[23230279]

Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm

A, N, F1

N/A

Cytoplasm

Cytoplasmic

no

yes

N

69

0.1

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

10

30

PROV_ECOLI

P14175

proV

b2677 JW2652

cytoplasmic peripheral IM protein, Glycine betaine/proline betaine transport system ATP-binding ProV

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)

Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279]

[16079137] [23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

Soluble IM Fraction

Cell inner membrane

Cytoplasmic

yes

yes

N

24

0.108

CYT

0

-0.200913

-

0

0

N

i

CytoplasmicMembrane

10

73

FECE_ECOLI

P15031

fecE

b4287 JW4247

cytoplasmic peripheral IM protein, Fe(3+) dicitrate transport ATP-binding FecE

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cell inner membrane; Peripheral membrane protein

Cytoplasmic

yes

yes

N

62

0.148

CYT

0

-0.200913

-

0

0

N

i

CytoplasmicMembrane

10

4

FLIN_ECOLI

P15070

fliN

flaN motD

b1946 JW1930

Flagellar cytoplasmic peripheral IM protein, motor switch FliN

Flaggelar C-ring proteins - FliG, FliM, and FliN.

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cell inner membrane; Peripheral membrane protein; Cytoplasmic side Bacterial flagellum basal body

Cytoplasmic

flagellum

no

yes

N

35

0.119

CYT

0

-0.200913

-

0

0

N

i

CytoplasmicMembrane

10

107

MALQ_ECOLI

P15977

malQ

malA

b3416 JW3379

cytoplasmic peripheral IM protein, 4-alpha-glucanotransferase MalQ

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)

Experimentally detected by proteomics analysis of E.coli membrane fraction (Bernsel et al 2009). No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Potential peripherally associated protein facing the cytoplasm or integral inner membrane protein.

[19766000]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

Soluble IM Fraction

Cytoplasm

Cytoplasmic

yes

yes

N

23

0.214

SpI

22

0.437923

-

0

0

N

i

Cytoplasmic

10

31

SDHL_ECOLI

P16095

sdaA

b1814 JW1803

cytoplasmic peripheral IM protein, L-serine dehydratase 1 SdaA

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

[23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

IM (predicted)

Cytoplasmic

yes

yes

N

20

0.136

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

9

71

HYCE_ECOLI

P16431

hycE

hevE

b2721 JW2691

cytoplasmic peripheral IM protein, Formate hydrogenlyase subunit 5 HycE

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)

Nickel-containing large subunit of Hydrogenase 3 (hycABCDEF-GHI) that forms a loose association with the molyb- doenzyme formate dehydrogenase-H on the cytoplasmic face of the cytoplasmic membrane, where together they make up the formate hydrogenlyase complex.

[1625581]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

IM (predicted)

Cytoplasmic

yes

yes

N

27

0.119

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

9

7

SYP_ECOLI

P16659

proS

drpA

b0194 JW0190

cytoplasmic peripheral IM protein, Proline--tRNA ligase ProS

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

Trigger factor-sensitive aggregation.

[23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasm

Cytoplasmic

TF

yes

yes

N

19

0.254

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

10

16

CYSA_ECOLI

P16676

cysA

b2422 JW2415

cytoplasmic peripheral IM protein, Sulfate/thiosulfate import ATP-binding CysA

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279]

[23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cell inner membrane

Cytoplasmic

yes

yes

N

33

0.112

CYT

0

-0.200913

-

0

0

N

i

CytoplasmicMembrane

7

14

PHNC_ECOLI

P16677

phnC

b4106 JW4067

cytoplasmic peripheral IM protein, Phosphonates import ATP-binding PhnC

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cell inner membrane

Cytoplasmic

yes

yes

N

23

0.137

CYT

0

-0.200913

-

0

0

N

i

CytoplasmicMembrane

10

10

PHNK_ECOLI

P16678

phnK

b4097 JW5727

cytoplasmic peripheral IM protein, Putative phosphonates utilization ATP-binding PhnK

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (By similarity)

Homolog to import ATP-binding protein MalK. Homolog to import ATP-binding protein MalK.

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasmic

yes

yes

N

23

0.117

CYT

0

-0.200913

-

0

0

N

i

CytoplasmicMembrane

7

6

PHNL_ECOLI

P16679

phnL

b4096 JW4057

cytoplasmic peripheral IM protein, Alpha-D-ribose 1-methylphosphonate 5-triphosphate synthase subunit PhnL

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (By similarity)

Homolog to import ATP-binding protein MalK. Homolog to import ATP-binding protein MalK.

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasmic

yes

yes

N

34

0.263

CYT

0

-0.200913

-

0

0

N

i

CytoplasmicMembrane

9

8

PHNP_ECOLI

P16692

phnP

b4092 JW4053

cytoplasmic peripheral IM protein, Phosphoribosyl 1,2-cyclic phosphate phosphodiesterase PhnP

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

[8335257]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Periplasmic (Experimental)

yes

yes

N

21

0.153

CYT

0

-0.200913

-

0

0

Y

n3-10c15/16o

Cytoplasmic

9

RHSB_ECOLI

P16917

rhsB

b3482 JW5679

cytoplasmic peripheral IM protein, RhsB

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

[23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasmic

Rhs Element

no

no

N

46

0.116

CYT

0

-0.200913

-

0

0

N

i

Extracellular

9

GLMS_ECOLI

P17169

glmS

b3729 JW3707

cytoplasmic peripheral IM protein, Glutamine--fructose-6-phosphate aminotransferase GlmS

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

Trigger factor-sensitive aggregation.

[23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

IM (predicted)

Cytoplasm

Cytoplasmic

TF

no

yes

N

59

0.122

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

10

91

MURG_ECOLI

P17443

murG

b0090 JW0088

cytoplasmic peripheral IM protein, UDP-N-acetylglucosamine--N-acetylmuramyl-(pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase MurG

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)

Pyrophosphoryl-undecaprenol N-acetylglucosamine transferase. Murein synthesis peripheral membrane protein interacting with cardiolipin. [8449890] [10892798] Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279]

[10892798] [23230279] [8449890]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cell inner membrane

Membrane associated (Experimental)

yes

yes

N

27

0.156

TMH

0

3.61924

-

0

0

N

i

CytoplasmicMembrane

10

48

BETA_ECOLI

P17444

betA

b0311 JW0303

cytoplasmic peripheral IM protein, Oxygen-dependent choline dehydrogenase BetA

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)

Exhibites aberrant localization by microscopy in the absence of YidC. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279]

[23230279] [3512525]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cell membrane

Cytoplasmic

no

yes

N

19

0.163

CYT

0

-0.200913

-

0

0

N

i

CytoplasmicMembrane

10

8

CYSH_ECOLI

P17854

cysH

b2762 JW2732

cytoplasmic peripheral IM protein, Phosphoadenosine phosphosulfate reductase CysH

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

[23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasm

Cytoplasmic

yes

yes

N

59

0.168

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

10

11

MURC_ECOLI

P17952

murC

b0091 JW0089

cytoplasmic peripheral IM protein, UDP-N-acetylmuramate--L-alanine ligase MurC

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Potential) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)

Trigger factor-sensitive aggregation. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279]

[19766000] [23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

Soluble IM Fraction +

Cytoplasm

Cytoplasmic

TF

yes

yes

N

41

0.228

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

9

76

NADE_ECOLI

P18843

nadE

efg ntrL

b1740 JW1729

cytoplasmic peripheral IM protein, NH(3)-dependent NAD(+) synthetase NadE

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

[23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasmic

yes

yes

N

20

0.146

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

9

NARY_ECOLI

P19318

narY

b1467 JW1462

cytoplasmic peripheral IM protein, Respiratory nitrate reductase 2 beta chain NarY

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279]

[23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cell membrane; Peripheral membrane protein

Cytoplasmic

yes

yes

N

32

0.124

CYT

0

-0.200913

-

0

0

N

i

CytoplasmicMembrane

10

14

NARZ_ECOLI

P19319

narZ

b1468 JW1463

cytoplasmic peripheral IM protein, Respiratory nitrate reductase 2 alpha chain NarZ

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279]

[23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

Soluble IM Fraction

Cell membrane; Peripheral membrane protein

Cytoplasmic

yes

yes

N

20

0.145

CYT

0

-0.200913

-

0

0

N

i

CytoplasmicMembrane

10

FADB_ECOLI

P21177

fadB

oldB

b3846 JW3822

cytoplasmic peripheral IM protein, Fatty acid oxidation complex subunit alpha FadB

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

Trigger factor-sensitive aggregation.

[23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasmic

TF

yes

yes

N

31

0.13

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

9

15

RNR_ECOLI

P21499

rnr

vacB yjeC

b4179 JW5741

cytoplasmic peripheral IM protein, Ribonuclease R Rnr

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

[23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasm

Cytoplasmic

yes

yes

N

3

0.1

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

9

45

SRMB_ECOLI

P21507

srmB

rbaB rhlA

b2576 JW2560

cytoplasmic peripheral IM protein, ATP-dependent RNA helicase SrmB

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

Assembly cofactor of ribosome; associate with the 50S subunit.

[15919657] [23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

Soluble IM Fraction

Cytoplasm

Cytoplasmic

yes

yes

N

33

0.109

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

10

68

RNE_ECOLI

P21513

rne

ams hmp1

b1084 JW1071

cytoplasmic peripheral IM protein, Ribonuclease E Rne

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)

Membrane-binding specificity of NRne was confirmed using circular dichroism difference spectroscopy. RnE binds specifically to anionic phospholipids through electrostatic interactions through N-terminal region (NRne, residues 1-499). Identified DsbC substrate [19953543] Identified DsbA substrate [19953543] [2011493] Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279] Part of membrane-associating assemblies that coil around the periphery of the cell when visualized by immunofluorescence microscopy. These assemblies co-localize with the RNA degradosome and participate in the RNA degradation process. [24438330]

[15911532] [18976283] [2011493] [22509045] [23230279] [24438330]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

Soluble IM Fraction

Cytoplasm

Cytoplasmic (Experimental)

yes

yes

N

62

0.101

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

10

KPYK2_ECOLI

P21599

pykA

b1854 JW1843

cytoplasmic peripheral IM protein, Pyruvate kinase II PykA

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

[23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasmic

yes

yes

N

43

0.131

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

9

43

DBPA_ECOLI

P21693

dbpA

b1343 JW1337

cytoplasmic peripheral IM protein, ATP-dependent RNA helicase DbpA

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

Assembly cofactor of ribosome; Fragment of 23S rRNA. Assembly cofactor of ribosome; Fragment of 23S rRNA. [21529161]

[21529161]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasm

Cytoplasmic

yes

yes

N

32

0.341

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

9

12

SYD_ECOLI

P21889

aspS

tls

b1866 JW1855

cytoplasmic peripheral IM protein, Aspartate--tRNA ligase AspS

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)

SecY-interacting protein. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279]

[19139097] [23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasm

Cytoplasmic

yes

yes

N

27

0.104

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

10

81

ASNB_ECOLI

P22106

asnB

b0674 JW0660

cytoplasmic peripheral IM protein, Asparagine synthetase B AsnB

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

Copurifies with trigger factor in pull down assays.

[23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasmic

TF

yes

yes

N

37

0.141

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

9

CYSQ_ECOLI

P22255

cysQ

amtA

b4214 JW4172

cytoplasmic peripheral IM protein, 3'(2'),5'-bisphosphate nucleotidase CysQ

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)

Identified in the inner membrane by biochemical fractionation followed by two-dimensional gel electrophoresis and tandem mass spectrometry. [15911532] [15911532]

[15911532]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cell inner membrane

Membrane associated (Experimental)

no

yes

N

40

0.152

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

9

83

GABT_ECOLI

P22256

gabT

b2662 JW2637

cytoplasmic peripheral IM protein, 4-aminobutyrate aminotransferase GabT

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

[23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasmic

yes

yes

N

65

0.16

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

9

77

ADD_ECOLI

P22333

add

b1623 JW1615

cytoplasmic peripheral IM protein, Adenosine deaminase Add

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

[23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasmic

yes

yes

N

40

0.118

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

9

15

MUKB_ECOLI

P22523

mukB

b0924 JW0907

cytoplasmic nucleoid peripheral IM protein, Chromosome partition MukB

Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (Experimental) Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (Probable)

[11886550] [16982609] [20921377] [22380631] [23230279]

Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm

A, N, F1

N/A

Cytoplasm; nucleoid

Cytoplasmic

yes

yes

N

46

0.13

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

10

MUKE_ECOLI

P22524

mukE

kicA ycbA

b0923 JW0906

cytoplasmic nucleoid peripheral IM protein, Chromosome partition MukE

Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (Experimental)

[16982609] [20921377] [23230279]

Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm

A, N, F1

N/A

Cytoplasm; nucleoid

Cytoplasmic

yes

yes

N

25

0.113

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

9

85

LIVF_ECOLI

P22731

livF

b3454 JW3419

cytoplasmic peripheral IM protein, High-affinity branched-chain amino acid transport ATP-binding LivF

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (By similarity)

Homolog to import ATP-binding protein MalK. Homolog to import ATP-binding protein MalK.

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasmic

yes

yes

N

42

0.111

CYT

0

-0.200913

-

0

0

N

i

CytoplasmicMembrane

7

15

FECI_ECOLI

P23484

fecI

b4293 JW4253

cytoplasmic nucleoid peripheral IM protein, Probable RNA polymerase sigma factor FecI

Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (Experimental)

Interacts with FecR at the inner membrane propably through the N-terminal region of FecR (1-85) which is cytoplasmic[16718597]. FecR spans the cytoplasmic membrane once, interacts in the periplasm with its C-terminal region with FecA occupied by ferric citrate, and transmits the information through the cytoplasmic membrane into the cytoplasm, where it converts FecI into an active sigma factor[9573190]. FecI specifically binds FecR (a transmembrane protein) and that this binding is necessary for FecI to function as a sigma factor[12057967]. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279]

[12057967] [23230279]

Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm

A, N, F1

N/A

Cytoplasmic

no

yes

N

23

0.11

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

10

16

PPSA_ECOLI

P23538

ppsA

pps

b1702 JW1692

cytoplasmic peripheral IM protein, Phosphoenolpyruvate synthase PpsA

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

One very weak transmembrane region predicted. Trigger factor-sensitive aggregation.

[23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

Soluble IM Fraction

Cytoplasmic

TF

yes

yes

N

56

0.169

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

9

48

PSS_ECOLI

P23830

pssA

pss

b2585 JW2569

cytoplasmic peripheral IM protein, CDP-diacylglycerol--serine O-phosphatidyltransferase PssA

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

Phospholipid metabolism complex PlsB-PssA-YbgC-Acp. Trigger factor-sensitive aggregation. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279]

[23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasm Cell inner membrane; Peripheral membrane protein; Cytoplasmic side

Cytoplasmic

TF

yes

yes

N

48

0.108

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

10

35

PPIB_ECOLI

P23869

ppiB

b0525 JW0514

cytoplasmic peripheral IM protein, Peptidyl-prolyl cis-trans isomerase B PpiB

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

[23230279] [9268657]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasm

Cytoplasmic (Experimental)

yes

yes

N

10

0.101

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

10

64

HEMH_ECOLI

P23871

hemH

popA visA

b0475 JW0464

cytoplasmic peripheral IM protein, Ferrochelatase HemH

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

[23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasm

Cytoplasmic

yes

yes

N

63

0.136

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

10

15

FEPC_ECOLI

P23878

fepC

b0588 JW0580

cytoplasmic peripheral IM protein, Ferric enterobactin transport ATP-binding FepC

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cell inner membrane

Cytoplasmic

yes

yes

N

63

0.171

CYT

0

-0.200913

-

0

0

N

i

CytoplasmicMembrane

9

17

GSA_ECOLI

P23893

hemL

gsa popC

b0154 JW0150

cytoplasmic peripheral IM protein, Glutamate-1-semialdehyde 2,1-aminomutase HemL

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

[23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasm

Cytoplasmic

yes

yes

N

26

0.222

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

9

33

ARGE_ECOLI

P23908

argE

b3957 JW3929

cytoplasmic peripheral IM protein, Acetylornithine deacetylase ArgE

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

[23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasm

Cytoplasmic

yes

yes

N

23

0.163

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

9

42

RFAC_ECOLI

P24173

rfaC

rfa-2 waaC yibC

b3621 JW3596

cytoplasmic peripheral IM protein, polysaccharide heptosyltransferase 1 RfaC

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

[23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasmic

yes

yes

N

50

0.195

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

9

51

ACCC_ECOLI

P24182

accC

fabG

b3256 JW3224

cytoplasmic peripheral IM protein, Biotin carboxylase AccC

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

Trigger factor-sensitive aggregation [19737520].

[23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasmic

TF

yes

yes

N

36

0.117

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

9

29

RADA_ECOLI

P24554

radA

sms

b4389 JW4352

cytoplasmic nucleoid peripheral IM protein, DNA repair RadA

Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (Experimental)

[23230279]

Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm

A, N, F1

N/A

Cytoplasmic

yes

yes

N

41

0.199

CYT

0

-0.200913

-

0

0

N

i

CytoplasmicMembrane

9

29

FRMA_ECOLI

P25437

frmA

adhC

b0356 JW0347

cytoplasmic peripheral IM protein, S-(hydroxymethyl)glutathione dehydrogenase FrmA

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

[23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

IM (predicted)

Cytoplasm

Cytoplasmic

yes

yes

N

50

0.109

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

10

71

HFLX_ECOLI

P25519

hflX

b4173 JW4131

cytoplasmic peripheral IM protein, GTPase HflX

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (By similarity) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

The hflALocus was found to comprise three genes, hflX, hflK, and hflC. HflK and HflC are membrane proteins and form a mem- brane-bound protease complex along with FtsH(HflB), and they are thought to act as modulators of the function of HflB. The role of the HflX GTPase/ATPase is unclear but has been shown to bind to ribosomes. When overexpressed, a portion of HflXProtein appeared in the membrane fraction, while larger amounts fractionated with the cytoplasmic pool (unpublished data cited in [19181811]). In Corynebacterium glutamicum, HflX has been found to occur equally in the cytoplasm and in the membrane fractions ([15978086]). Has no transmembrane region either in E. coli or in C. glutamicum. Trigger factor-sensitive aggregation. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279]

[15978086] [19181811] [23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasm

Cytoplasmic

TF

yes

yes

N

52

0.116

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

8

52

MNME_ECOLI

P25522

mnmE

thdF trmE

b3706 JW3684

cytoplasmic peripheral IM protein, tRNA modification GTPase MnmE

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)

A GTPase that exhibits high intrinsic GTPase hydrolysis rate,Low affinity for GTP, and extremely low affinity for GDP Can form self-assemblies. Is a cytoplasmic protein partially associated with the inner membrane. Involved in the biosynthesis of the hypermodified nucleoside 5-methylaminomethyl-2-thiouridine, which is found in the wobble position of some tRNAs. Essential for viability depending on genetic background. Fluorescent fusions of MnmE have been monitored via fluorescence microscopy. MnmE located predominantly beside the membrane.

[10601028] [17272300]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

Soluble IM Fraction

Cytoplasm

Cytoplasmic

yes

yes

N

47

0.153

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

9

89

GABD_ECOLI

P25526

gabD

b2661 JW2636

cytoplasmic peripheral IM protein, Succinate-semialdehyde dehydrogenase GabD

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

[23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasmic

no

yes

N

28

0.124

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

9

60

UBIH_ECOLI

P25534

ubiH

visB

b2907 JW2875

cytoplasmic peripheral IM protein, 2-octaprenyl-6-methoxyphenol hydroxylase UbiH

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)

The biosynthesis of UQ is studied in great detail in bacteria and yeast but only to a limited extent in animal tissues. The UQ biosynthetic enzymes may constitute a complex that is tightly bound to the membrane.

[11583838]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasmic

yes

yes

N

42

0.38

SpI

25

1.25365

-

0

0

Y

n3-13c18/19o

Unknown

6

15

HTRL_ECOLI

P25666

htrL

yibB

b3618 JW5644

cytoplasmic peripheral IM protein, HtrL

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)

Experimentally detected by proteomics analysis of E.coli membrane fraction (Bernsel et al 2009). No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Potential peripherally associated protein facing the cytoplasm or integral inner membrane protein.

[19766000]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

Soluble IM Fraction

Cytoplasmic

no

yes

N

45

0.131

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

8

50

RFAG_ECOLI

P25740

rfaG

pcsA waaG

b3631 JW3606

cytoplasmic peripheral IM protein, polysaccharide core biosynthesis RfaG

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Potential) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)

Experimentally detected by proteomics analysis of E.coli membrane fraction (Bernsel et al 2009). No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Potential peripherally associated protein facing the cytoplasm or integral inner membrane protein. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279]

[19766000] [23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

Soluble IM Fraction

Cytoplasmic

yes

yes

N

25

0.118

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

9

30

RFAP_ECOLI

P25741

rfaP

waaP

b3630 JW3605

cytoplasmic peripheral IM protein, polysaccharide core heptose RfaP

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

[23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

Soluble IM Fraction

Cytoplasmic

yes

yes

N

20

0.155

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

9

33

RFAQ_ECOLI

P25742

rfaQ

waaQ

b3632 JW3607

cytoplasmic peripheral IM protein, polysaccharide core heptosyltransferase RfaQ

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

[23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasmic

yes

yes

N

25

0.11

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

8

33

HFLD_ECOLI

P25746

hflD

ycfC

b1132 JW5165

cytoplasmic peripheral IM protein, High frequency lysogenization HflD

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)

Downregulates lambda lysogenization;Interacts with cIIProtein; first gene in purB operon Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279]

[11278968] [23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

Soluble IM Fraction

Cytoplasm

Cytoplasmic

yes

yes

N

54

0.157

CYT

0

-0.200913

-

0

0

N

i

Unknown

2

19

RHLE_ECOLI

P25888

rhlE

b0797 JW0781

cytoplasmic peripheral IM protein, ATP-dependent RNA helicase RhlE

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

Assembly cofactor of ribosome; associates with the 70S subunit. Overex<x>pression of protein suppresses defects in deletion of DeaD. [18083833]

[18083833] [23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasm

Cytoplasmic

yes

yes

N

46

0.14

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

9

62

WAAU_ECOLI

P27242

waaU

rfaK waaK

b3623 JW3598

cytoplasmic peripheral IM protein, polysaccharide 1,2-N-acetylglucosaminetransferase WaaU

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

[1657881]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Membrane; Peripheral membrane protein

Cytoplasmic

no

yes

N

33

0.113

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

8

PLSX_ECOLI

P27247

plsX

b1090 JW5156

cytoplasmic peripheral IM protein, Phosphate acyltransferase PlsX

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

[23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasm

Cytoplasmic

yes

yes

N

24

0.213

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

9

15

GCST_ECOLI

P27248

gcvT

b2905 JW2873

cytoplasmic peripheral IM protein, Aminomethyltransferase GcvT

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

[23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasmic

no

yes

N

31

0.116

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

9

101

ARGK_ECOLI

P27254

argK

ygfD

b2918 JW2885

cytoplasmic peripheral IM protein, GTPase ArgK ArgK

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)

Appears to phosphorylate the periplasmic binding proteins of the lysine, arginine, ornithine transport system as well as the arginine, ornithine transport system[2136858].

[2136858]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasmic

no

yes

N

26

0.12

CYT

0

-0.200913

-

0

0

N

i

CytoplasmicMembrane

10

23

NADR_ECOLI

P27278

nadR

nadI

b4390 JW5800

cytoplasmic nucleoid peripheral IM protein, Trifunctional NAD biosynthesis/regulator NadR

Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (Experimental) Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (Probable)

May loosely associate with the membrane through its interaction with the membrane-bound pnuC (Nicotinamide riboside transporter). In combination with corepressor (NAD?), the cytoplasmic form of nadR would be capable of acting as a transcriptional repressor. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279]

[10464228] [23138451] [23230279]

Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm

A, N, F1

N/A

Cell membrane; Peripheral membrane protein Cytoplasm

Cytoplasmic

yes

yes

N

39

0.131

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

8

39

OPDA_ECOLI

P27298

prlC

opdA

b3498 JW3465

cytoplasmic peripheral IM protein, Oligopeptidase A PrlC

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

[23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasmic

yes

yes

N

26

0.115

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

10

94

TKT1_ECOLI

P27302

tktA

tkt

b2935 JW5478

cytoplasmic peripheral IM protein, Transketolase 1 TktA

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

Trigger factor-sensitive aggregation.

[23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasmic

TF

yes

yes

N

25

0.124

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

9

32

STHA_ECOLI

P27306

sthA

sth udhA

b3962 JW5551

cytoplasmic peripheral IM protein, Soluble pyridine nucleotide transhydrogenase SthA

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

SthA is bound on the inner mebrane through PntAB heterodimer. Trigger factor-sensitive aggregation. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279]

[14660605] [23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasm

Cytoplasmic

TF

yes

yes

N

22

0.133

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

10

22

ACSA_ECOLI

P27550

acs

yfaC

b4069 JW4030

cytoplasmic peripheral IM protein, Acetyl-coenzyme A synthetase Acs

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (By similarity) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

Homologous to FadD13 protein of Mycobacterium that is peripheral. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279]

[22560731] [23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasmic

yes

yes

N

27

0.136

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

9

62

WECG_ECOLI

P27836

wecG

rffM

b3794 JW3770

cytoplasmic peripheral IM protein, UDP-N-acetyl-D-mannosaminuronic acid transferase WecG

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

[23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

Soluble IM Fraction

Cytoplasmic

yes

yes

N

27

0.16

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

8

35

TREC_ECOLI

P28904

treC

olgH

b4239 JW4198

cytoplasmic peripheral IM protein, Trehalose-6-phosphate hydrolase TreC

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

Trigger factor-sensitive aggregation.

[23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasm

Cytoplasmic

TF

yes

yes

N

60

0.145

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

10

9

ALR2_ECOLI

P29012

dadX

alnB dadB

b1190 JW1179

cytoplasmic peripheral IM protein, Alanine racemase, catabolic DadX

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

[23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasmic

yes

yes

N

25

0.206

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

10

2

METN_ECOLI

P30750

metN

abc

b0199 JW0195

cytoplasmic peripheral IM protein, Methionine import ATP-binding MetN

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279]

[23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cell inner membrane

Cytoplasmic

yes

yes

N

42

0.139

CYT

0

-0.200913

-

0

0

N

i

CytoplasmicMembrane

9

23

RNB_ECOLI

P30850

rnb

b1286 JW1279

cytoplasmic nucleoid peripheral IM protein, Exoribonuclease 2 Rnb

Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (Experimental)

Membrane association via an amino-terminal amphipathic helix is required for the cellular organization and function of RNase II [23344958] Part of membrane-associating assemblies that coil around the periphery of the cell when visualized by immunofluorescence microscopy. These assemblies co-localize with the RNA degradosome and participate in the RNA degradation process. [24438330]

[23344958] [24438330]

Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm

A, N, F1

N/A

Cytoplasm

Cytoplasmic

yes

yes

N

23

0.181

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

10

48

MODF_ECOLI

P31060

modF

phrA

b0760 JW0743

cytoplasmic peripheral IM protein, ABC transporter ATP-binding ModF

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cell inner membrane

Cytoplasmic

no

yes

N

34

0.221

CYT

0

-0.200913

-

0

0

N

i

CytoplasmicMembrane

9

33

POTG_ECOLI

P31134

potG

b0855 JW5818

cytoplasmic peripheral IM protein, Putrescine transport ATP-binding PotG

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cell inner membrane

Cytoplasmic

yes

yes

N

35

0.11

CYT

0

-0.200913

-

0

0

N

i

CytoplasmicMembrane

7

11

THIQ_ECOLI

P31548

thiQ

yabJ

b0066 JW0065

cytoplasmic peripheral IM protein, Thiamine import ATP-binding ThiQ

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (By similarity)

Two very weak TM predictions; membrane associated activity known. Part of the ABC transporter complex ThiBPQ involved in thiamine import. Responsible for energy coupling to the transport system [12175925]

[12175925]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cell inner membrane

Cytoplasmic

yes

yes

N

25

0.144

CYT

0

-0.200913

-

0

0

N

i

CytoplasmicMembrane

10

8

CAIC_ECOLI

P31552

caiC

yaaM

b0037 JW0036

cytoplasmic peripheral IM protein, Crotonobetaine/carnitine--CoA ligase CaiC

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (By similarity)

Homolog to FadD13 protein of Mycobacterium that is peripheral.

[22560731]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasmic

yes

yes

N

18

0.105

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

9

OTSA_ECOLI

P31677

otsA

b1896 JW5312

cytoplasmic peripheral IM protein, Trehalose-6-phosphate synthase OtsA

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)

Experimentally detected by proteomics analysis of E.coli membrane fraction (Bernsel et al 2009). No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Potential peripherally associated protein facing the cytoplasm or integral inner membrane protein.

[19766000]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

Soluble IM Fraction

Cytoplasmic

yes

yes

N

19

0.248

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

10

30

NUOF_ECOLI

P31979

nuoF

b2284 JW2279

cytoplasmic peripheral IM protein, NADH-quinone oxidoreductase subunit F NuoF

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

NADH dehydrogenase I is composed of 13 different subunits. Subunits nuoCD, E, F, and G constitute the peripheral sector of the complex.cThermus homologue: Q56222 (NQO1_THET8) Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279]

[15919657] [23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

IM (predicted)

Cytoplasmic

yes

yes

N

57

0.101

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

9

11

TYPA_ECOLI

P32132

typA

bipA yihK

b3871 JW5571

cytoplasmic peripheral IM protein, GTP-binding TypA/BipA

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Potential) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)

Trigger factor-sensitive aggregation. Experimentally detected by proteomics analysis of E.coli membrane fraction (Bernsel et al 2009). No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Potential peripherally associated protein facing the cytoplasm or integral inner membrane protein. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279]

[19766000] [23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

Soluble IM Fraction

Cytoplasmic

TF

yes

yes

N

37

0.112

CYT

0

-0.200913

-

0

0

N

i

CytoplasmicMembrane

7

88

PTFLA_ECOLI

P32155

frvA

yiiK

b3900 JW3871

cytoplasmic peripheral IM protein, PTS system fructose-like EIIA component FrvA

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (By similarity)

Fructose-like phosphotransferase enzyme IIA component

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasm

Cytoplasmic

no

yes

N

19

0.135

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

9

68

PTFX2_ECOLI

P32670

ptsA

frwA yijH

b3947 JW5555

cytoplasmic peripheral IM protein, Multiphosphoryl transfer 2 PtsA

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Potential)

Multiphosphoryl transfer protein 2

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasm

Cytoplasmic

no

yes

N

18

0.56

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

9

68

PTFB3_ECOLI

P32676

frwD

yijN

b3953 JW3925

cytoplasmic peripheral IM protein, PTS system fructose-like EIIB component 3 FrwD

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Potential)

Sequence analysis indicates that an frwD encoded protein has similarity to the IIB domains of the PTS Enzymes II specific for fructose.

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasm

Cytoplasmic

yes

yes

N

21

0.559

CYT

0

-0.200913

-

0

0

Y

n4-12c17/18o

Unknown

6

43

RLUF_ECOLI

P32684

rluF

yjbC

b4022 JW3982

cytoplasmic peripheral IM protein, 23S rRNA pseudouridine(2604) synthase RluF

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

[23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasmic

yes

yes

N

25

0.109

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

9

DUSA_ECOLI

P32695

dusA

yjbN

b4049 JW5950

cytoplasmic peripheral IM protein, tRNA-dihydrouridine(20/20a) synthase DusA

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)

Experimentally detected by proteomics analysis of E.coli membrane fraction (Bernsel et al 2009). No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Potential peripherally associated protein facing the cytoplasm or integral inner membrane protein.

[19766000]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

Soluble IM Fraction

Cytoplasmic

yes

yes

N

27

0.108

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

9

ALSA_ECOLI

P32721

alsA

yjcW

b4087 JW4048

cytoplasmic peripheral IM protein, D-allose import ATP-binding AlsA

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

Soluble IM Fraction

Cell inner membrane

Cytoplasmic

yes

yes

N

21

0.291

CYT

0

-0.200913

-

0

0

N

i

CytoplasmicMembrane

10

18

GCSP_ECOLI

P33195

gcvP

b2903 JW2871

cytoplasmic peripheral IM protein, Glycine dehydrogenase GcvP

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

[23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasmic

yes

yes

N

60

0.111

CYT

0

-0.200913

-

0

1

N

o751-770i

Cytoplasmic

9

21

LLDD_ECOLI

P33232

lldD

lctD

b3605 JW3580

cytoplasmic peripheral IM protein, L-lactate dehydrogenase LldD

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

LldD is a cytochrome. There is a strong experimental indication that it is a membrane bound protein although no transmembrane helix is predicted [18473]. [8407843] [6997721] [18473] Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279]

[15919657] [18473] [23230279] [6997721] [8407843]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

IM (predicted-detected)

Cell inner membrane

Membrane associated (Experimental)

yes

yes

N

32

0.129

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

9

4

MLRA_ECOLI

P33358

mlrA

yehV

b2127 JW2115

cytoplasmic nucleoid peripheral IM protein, HTH-type transcriptional regulator MlrA

Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (Probable)

MlrA has regulatory role in transcription activation of csgD, which is the master regulator of biofilm formation.

[20874755]

Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm

A, N, F1

IM (predicted)

Cytoplasmic

yes

yes

N

23

0.15

CYT

0

-0.200913

-

0

1

N

o135-155i

Cytoplasmic

9

114

YEHX_ECOLI

P33360

yehX

b2129 JW2117

cytoplasmic peripheral IM protein, Glycine betaine uptake system ATP-binding YehX

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (By similarity)

Homolog to import ATP-binding protein MalK.

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasmic

yes

yes

N

42

0.103

CYT

0

-0.200913

-

0

0

N

i

CytoplasmicMembrane

7

8

TKT2_ECOLI

P33570

tktB

b2465 JW2449

cytoplasmic peripheral IM protein, Transketolase 2 TktB

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

[23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasmic

yes

yes

N

24

0.145

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

9

11

NIKD_ECOLI

P33593

nikD

b3479 JW3444

cytoplasmic peripheral IM protein, Nickel import ATP-binding NikD

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cell inner membrane

Cytoplasmic

yes

yes

N

48

0.168

CYT

0

-0.200913

-

0

0

N

i

CytoplasmicMembrane

7

45

NIKE_ECOLI

P33594

nikE

b3480 JW3445

cytoplasmic peripheral IM protein, Nickel import ATP-binding NikE

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cell inner membrane

Cytoplasmic

yes

yes

N

68

0.149

CYT

0

-0.200913

-

0

0

N

i

CytoplasmicMembrane

9

41

NUOCD_ECOLI

P33599

nuoC

nuoCD nuoD

b2286 JW5375

cytoplasmic peripheral IM protein, NADH-quinone oxidoreductase subunit C/D NuoC

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)

Trigger factor-sensitive aggregation. Identified in nucleoids collected by sucrose gradient centrifugation and analyzed by liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS). [21541338] [7607227] Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279]

[15919657] [16079137] [23230279] [7607227]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

IM (predicted)

Cytoplasm

Cytoplasmic (Experimental)

TF

yes

yes

N

39

0.108

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

9

17

NUOG_ECOLI

P33602

nuoG

b2283 JW2278

cytoplasmic peripheral IM protein, NADH-quinone oxidoreductase subunit G NuoG

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)

Trigger factor-sensitive aggregation. [11416161] Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279]

[11416161] [15919657] [16079137] [23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

Soluble IM Fraction

Cytoplasm

Cytoplasmic (Experimental)

TF

yes

yes

N

57

0.143

CYT

0

-0.200913

-

0

0

N

i

CytoplasmicMembrane

10

17

YEJF_ECOLI

P33916

yejF

b2180 JW2168

cytoplasmic peripheral IM protein, ABC transporter ATP-binding YejF

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (By similarity)

Homolog to import ATP-binding protein MalK.

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

Soluble IM Fraction

Cytoplasmic

yes

yes

N

35

0.139

CYT

0

-0.200913

-

0

0

N

i

CytoplasmicMembrane

9

CCMA_ECOLI

P33931

ccmA

yejW

b2201 JW5366

cytoplasmic peripheral IM protein, Cytochrome c biogenesis ATP-binding export CcmA

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cell inner membrane

Cytoplasmic

yes

yes

N

29

0.16

CYT

0

-0.200913

-

0

0

N

i

CytoplasmicMembrane

7

47

MQO_ECOLI

P33940

mqo

yojH

b2210 JW2198

cytoplasmic peripheral IM protein, Malate:quinone oxidoreductase Mqo

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

High signal PProbability and lipoP SPIPrediction; weak TmHMMPrediction definite membrane association according to literature. MQOIs a flavin adenine dinucleotide (FAD)- and lipid-dependent peripheral membrane protein catalyzing the oxidation ofL-malate to oxaloacetate. [11092847] [368072] Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279]

[11092847] [23230279] [368072]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

IM (predicted)

Membrane associated (Experimental)

yes

yes

Y

27

0.278

SpI

21

8.41159

-

0

0

Y

n4-15c21/22o

Unknown

6

17

AHPF_ECOLI

P35340

ahpF

b0606 JW0599

cytoplasmic peripheral IM protein, Alkyl hydroperoxide reductase subunit F AhpF

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Potential) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)

Experimentally detected by proteomics analysis of E.coli membrane fraction (Bernsel et al 2009). No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Potential peripherally associated protein facing the cytoplasm or integral inner membrane protein. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279] Disulfide bridge (345 348)

[19766000] [23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

Soluble IM Fraction

Cytoplasmic

yes

yes

N

35

0.108

CYT

0

-0.200913

-

0

0

N

i

CytoplasmicMembrane

7

76

WBBL_ECOLI

P36667

wbbL

b4571 JW2016 b2031

cytoplasmic peripheral IM protein, Rhamnosyltransferase WbbL

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)

Experimentally detected by proteomics analysis of E.coli membrane fraction (Bernsel et al 2009). No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Potential peripherally associated protein facing the cytoplasm or integral inner membrane protein.

[19766000]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

Soluble IM Fraction

Cytoplasmic

no

yes

N

51

0.111

CYT

0

-0.200913

-

0

0

N

i

Unknown

2

54

ZAPD_ECOLI

P36680

zapD

yacF

b0102 JW0099

cytoplasmic peripheral IM protein, Cell division ZapD

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)

Cell division factor that enhances FtsZ-ring assembly. Directly interacts with FtsZ and promotes bundling of FtsZ protofilaments, with a reduction in FtsZ GTPase activity. 22505682

22505682 [22505682]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasm

Cytoplasmic

yes

yes

N

39

0.116

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

8

80

ACNB_ECOLI

P36683

acnB

yacI yacJ

b0118 JW0114

cytoplasmic peripheral IM protein, Aconitate hydratase B AcnB

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

Trigger factor-sensitive aggregation. [10585860] Disulfide bond-containing protein [25664544]

[10585860] [23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasmic (Experimental)

TF

yes

yes

N

42

0.124

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

9

90

YADG_ECOLI

P36879

yadG

b0127 JW0123

cytoplasmic peripheral IM protein, ABC transporter ATP-binding YadG

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Potential)

Homolog to import ATP-binding protein MalK. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279]

[23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasmic

yes

yes

N

31

0.246

CYT

0

-0.200913

-

0

0

N

i

CytoplasmicMembrane

7

5

YADI_ECOLI

P36881

yadI

b0129 JW0125

cytoplasmic peripheral IM protein, Putative phosphotransferase enzyme IIA component YadI

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (By similarity)

Putative phosphotransferase enzyme IIA component yadI

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasm

Cytoplasmic

yes

yes

N

46

0.118

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

8

17

KDPF_ECOLI

P36937

kdpF

b4513 JW0687

cytoplasmic peripheral IM protein, Potassium-transporting ATPase KdpF subunit KdpF

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Potential)

KdpF, could be identified on high resolution SDS-polyacrylamide gels in addition to the subunits KdpA, KdpB, and KdpC. Furthermore, it could be demonstrated that KdpF remains associated with the purified complex. As determined by mass spectrometry, this pe [9789555]

[21890895] [9789555]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

IM (predicted)

Cell inner membrane

Membrane Anchored (Experimental)

no

yes

N

23

0.109

CYT

0

-0.200913

-

1

0

Y

n5-16c22/23o

CytoplasmicMembrane

10

29

YAGA_ECOLI

P37007

yagA

b0267 JW0260

cytoplasmic peripheral IM protein, YagA

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)

Experimentally detected by proteomics analysis of E.coli membrane fraction (Bernsel et al 2009). No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Potential peripherally associated protein facing the cytoplasm or integral inner membrane protein.

[19766000]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

Soluble IM Fraction

Cytoplasmic

Prophage Integrase (EcoGene)

no

no

N

29

0.125

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

8

2

FBPC_ECOLI

P37009

fbpC

afuC yagC

b0262 JW0254

cytoplasmic peripheral IM protein, Fe(3+) ions import ATP-binding FbpC

Homologous to MalK

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cell inner membrane

Cytoplasmic

Prophage Integrase (EcoGene)

yes

no

N

62

0.109

CYT

0

-0.200913

-

0

0

N

i

CytoplasmicMembrane

7

42

PT1P_ECOLI

P37177

ptsP

ygdF ygdO

b2829 JW2797

cytoplasmic peripheral IM protein, Phosphoenolpyruvate-dependent phosphotransferase system PtsP

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

Phosphoenolpyruvate-protein phosphotransferase ptsP Localization to the inner surface of the cytoplasmic membrane by immunoelectron microscopy of enzyme I of the phosphoenolpyruvate:sugar phosphotransferase system of Escherichia coli. [2644649]

[2644649]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasm

Cytoplasmic

yes

yes

N

33

0.185

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

9

9

PTKB_ECOLI

P37188

gatB

b2093 JW2077

cytoplasmic peripheral IM protein, PTS system galactitol-specific EIIB component GatB

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Potential)

Part of galactitol PTS permease complex. Strong Signal Peptide prediction. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279] Disulfide bond-containing protein [25664544]

[16963640] [23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasm

Cytoplasmic

no

yes

Y

20

0.743

SpI

19

8.62134

-

0

0

Y

n5-14c19/20o

Cytoplasmic

10

DPPF_ECOLI

P37313

dppF

dppE

b3540 JW3509

cytoplasmic peripheral IM protein, Dipeptide transport ATP-binding DppF

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279]

[23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

Soluble IM Fraction

Cell inner membrane

Cytoplasmic

yes

yes

N

53

0.133

CYT

0

-0.200913

-

0

0

N

i

CytoplasmicMembrane

9

24

LHGO_ECOLI

P37339

lhgO

ygaF

b2660 JW2635

cytoplasmic peripheral IM protein, L-2-hydroxyglutarate oxidase LhgO

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Potential) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)

Experimentally detected by proteomics analysis of E.coli membrane fraction (Bernsel et al 2009). No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Potential peripherally associated protein facing the cytoplasm or integral inner membrane protein. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279]

[19766000] [23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

IM (predicted), Soluble IM Fraction

Cytoplasmic

yes

yes

N

21

0.14

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

9

15

DHAM_ECOLI

P37349

dhaM

ycgC

b1198 JW5185

cytoplasmic peripheral IM protein, PEP-dependent dihydroxyacetone kinase, phosphoryl donor subunit DhaM

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Potential)

Part of Dihydroxyacetone kinase complex ([DhaM]2[DhaK]2[DhaL]). DhaMIs homologous to certain components of PTS: to a domain of EI, to HPr, and to the AB domains of EII. Part of Dihydroxyacetone kinase complex ([DhaM]2[DhaK]2[DhaL]). DhaMIs homologous to certain components of PTS: to a domain of EI, to HPr, and to the AB domains of EII. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279]

[23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasmic

no

yes

N

36

0.134

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

9

74

MENE_ECOLI

P37353

menE

b2260 JW2255

cytoplasmic peripheral IM protein, 2-succinylbenzoate--CoA ligase MenE

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (By similarity)

Homologous to FadD13 protein of Mycobacterium that is peripheral.

[22560731]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasmic

yes

yes

N

48

0.113

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

9

31

XYLG_ECOLI

P37388

xylG

b3567 JW3539

cytoplasmic peripheral IM protein, Xylose import ATP-binding XylG

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cell inner membrane

Cytoplasmic

yes

yes

N

20

0.188

CYT

0

-0.200913

-

0

0

N

i

CytoplasmicMembrane

7

48

RFAF_ECOLI

P37692

rfaF

waaF

b3620 JW3595

cytoplasmic peripheral IM protein, ADP-heptose--LPS heptosyltransferase 2 RfaF

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

[23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

Soluble IM Fraction

Cytoplasmic

yes

yes

N

42

0.134

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

9

24

WBBI_ECOLI

P37749

wbbI

yefG

b2034 JW2019

cytoplasmic peripheral IM protein, Beta-1,6-galactofuranosyltransferase WbbI WbbI

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)

Experimentally detected by proteomics analysis of E.coli membrane fraction (Bernsel et al 2009). No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Potential peripherally associated protein facing the cytoplasm or integral inner membrane protein.

[19766000]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

Soluble IM Fraction

Cytoplasm

Cytoplasmic

no

yes

N

42

0.102

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

8

14

WBBJ_ECOLI

P37750

wbbJ

yefH

b2033 JW2018

cytoplasmic peripheral IM protein, Putative polysaccharide biosynthesis O-acetyl transferase WbbJ

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)

Experimentally detected by proteomics analysis of E.coli membrane fraction (Bernsel et al 2009). No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Potential peripherally associated protein facing the cytoplasm or integral inner membrane protein.

[19766000]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

Soluble IM Fraction

Cytoplasmic

no

yes

N

24

0.107

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

9

WBBK_ECOLI

P37751

wbbK

yefI

b2032 JW2017

cytoplasmic peripheral IM protein, Putative glycosyltransferase WbbK

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279] Disulfide bond-containing protein [25664544]

[23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

Soluble IM Fraction

Cell inner membrane

Cytoplasmic

no

yes

N

21

0.119

CYT

0

-0.200913

-

0

0

N

i

Unknown

2

32

RLUB_ECOLI

P37765

rluB

yciL

b1269 JW1261

cytoplasmic peripheral IM protein, Ribosomal large subunit pseudouridine synthase B RluB

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

[23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasmic

yes

yes

N

32

0.101

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

9

YECC_ECOLI

P37774

yecC

b1917 JW1902

cytoplasmic peripheral IM protein, L-cystine transport system ATP-binding YecC

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

Soluble IM Fraction

Cell inner membrane

Cytoplasmic

yes

yes

N

34

0.123

CYT

0

-0.200913

-

0

0

N

i

CytoplasmicMembrane

7

37

FADK_ECOLI

P38135

fadK

ydiD

b1701 JW5910

cytoplasmic peripheral IM protein, Medium-chain fatty-acid--CoA ligase FadK

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (By similarity)

Homologous to FadD13 protein of Mycobacterium that is peripheral

[22560731]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cell membrane; Peripheral membrane protein

Cytoplasmic

no

yes

N

37

0.131

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

9

NFSB_ECOLI

P38489

nfsB

dprA nfnB nfsI ntr

b0578 JW0567

cytoplasmic peripheral IM protein, Oxygen-insensitive NAD(P)H nitroreductase NfsB

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

[12788749] [23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

IM (predicted)

Cytoplasmic (Experimental)

yes

yes

N

18

0.166

CYT

0

-0.200913

-

0

0

N

i

Unknown

2

86

YGGL_ECOLI

P38521

yggL

b2959 JW2926

cytoplasmic peripheral IM protein, YggL

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

[23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasmic

yes

yes

N

29

0.118

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

8

129

USPG_ECOLI

P39177

uspG

ybdQ yzzU

b0607 JW0600

cytoplasmic peripheral IM protein, Universal stress UP12 UspG

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Potential) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)

Experimentally detected by proteomics analysis of E.coli membrane fraction (Bernsel et al 2009). No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Potential peripherally associated protein facing the cytoplasm or integral inner membrane protein. [8740179] [9600841] [12071968] [16460009] Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279]

[12071968] [16460009] [19766000] [23230279] [8740179] [9600841]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

Soluble IM Fraction

Cytoplasmic (Experimental)

yes

yes

N

53

0.335

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

8

85

RSGA_ECOLI

P39286

rsgA

engC yjeQ

b4161 JW4122

ribosomal cytoplasmic peripheral IM protein, Small ribosomal subunit biogenesis GTPase RsgA

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm & ribosomal (Experimental)

Assembly cofactor of ribosome; associate with the 30S subunit.

[23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm, ribosomal

A, F1, r

N/A

Cytoplasm

Cytoplasmic

ribosome

yes

yes

N

53

0.108

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

10

YJFM_ECOLI

P39295

yjfM

b4185 JW4143

cytoplasmic peripheral IM protein, YjfM

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

YgiB and ygiC genes are expressed in the same operon as tolC and have homologues (yjfM and yjfC) located elsewhere on the chromosome. Mutational inactivation of the outer membrane channelTolC (a key component of multidrug efflux and type I secretion transporters) renders cells highly susceptible to antibiotics and leads to defects in secretion of protein toxins and the loss of TolC impairs cell division and growth in minimal glucose medium due to metabolic shutdown. The TolC-dependent phenotype was further exacerbated by the loss of ygiB and ygiC genes. YgiBC (and YjfMC) may have parallel but independent functions from TolC.

[20545840]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

IM (predicted)

Cytoplasmic

no

yes

N

55

0.107

CYT

0

-0.200913

-

0

0

N

i

Unknown

2

105

YJGR_ECOLI

P39342

yjgR

b4263 JW4220

cytoplasmic peripheral IM protein, YjgR

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279]

[23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

IM (predicted)

Cytoplasmic

yes

yes

N

58

0.139

CYT

0

-0.200913

-

0

0

N

i

Unknown

2

87

FHUF_ECOLI

P39405

fhuF

yjjS

b4367 JW4331

cytoplasmic peripheral IM protein, Ferric iron reductase FhuF

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

It has a large transmembrane helix in the middle.

[14756576]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

IM (predicted)

Cytoplasm Cell membrane; Peripheral membrane protein

Cytoplasmic

yes

yes

N

55

0.117

CYT

0

-0.200913

-

0

1

N

i91-112o

Cytoplasmic

10

29

RSMC_ECOLI

P39406

rsmC

yjjT

b4371 JW4333

cytoplasmic peripheral IM protein, Ribosomal RNA small subunit methyltransferase C RsmC

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

Ribosom related

[23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasm

Cytoplasmic

yes

yes

N

27

0.158

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

9

43

ADHP_ECOLI

P39451

adhP

yddN

b1478 JW1474

cytoplasmic peripheral IM protein, Alcohol dehydrogenase, propanol-preferring AdhP

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Potential)

No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius.

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

IM (predicted-detected)

Cytoplasmic

yes

yes

N

40

0.124

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

9

86

MTOX_ECOLI

P40874

solA

b1059 JW1046

cytoplasmic peripheral IM protein, N-methyl-L-tryptophan oxidase SolA

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Potential)

No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius.

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

IM (predicted-detected)

Cytoplasmic

yes

yes

N

16

0.149

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

9

73

AZOR_ECOLI

P41407

azoR

acpD

b1412 JW1409

cytoplasmic peripheral IM protein, FMN-dependent NADH-azoreductase AzoR

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)

Experimentally detected by proteomics analysis of E.coli membrane fraction (Bernsel et al 2009). No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Potential peripherally associated protein facing the cytoplasm or integral inner membrane protein.

[19766000]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

Soluble IM Fraction

Cytoplasmic

no

yes

N

19

0.135

CYT

0

-0.200913

-

0

0

Y

n4-13c18/19o

Cytoplasmic

9

108

UXAA_ECOLI

P42604

uxaA

ygjW

b3091 JW3062

cytoplasmic peripheral IM protein, Altronate dehydratase UxaA

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Potential)

No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius.

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

IM (predicted-detected)

Cytoplasmic

yes

yes

N

68

0.185

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

8

113

OBG_ECOLI

P42641

obgE

cgtA obg yhbZ

b3183 JW3150

cytoplasmic peripheral IM protein, GTPase ObgE/CgtA

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

Assembly cofactor of ribosome; associates 30S, 50S,16S, and 23S rRNA. [15292126] Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279]

[15292126] [21529161]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasm

Cytoplasmic

yes

yes

N

25

0.112

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

8

93

HRPA_ECOLI

P43329

hrpA

b1413 JW5905

cytoplasmic peripheral IM protein, ATP-dependent RNA helicase HrpA

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

[23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasmic

yes

yes

N

52

0.107

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

8

YCAK_ECOLI

P43340

ycaK

b0901 JW0884

cytoplasmic peripheral IM protein, YcaK

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Potential)

No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius.

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

IM (predicted-detected)

Cytoplasmic

no

yes

N

23

0.238

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

9

95

LPXH_ECOLI

P43341

lpxH

ybbF

b0524 JW0513

cytoplasmic peripheral IM protein, UDP-2,3-diacylglucosamine hydrolase LpxH

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

Phobius weak prediction of a hydrophobic TMIn the middle of sequence/Kyte-Doolittle predicts several hydrophobic patches

[20608695]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cell inner membrane

Cytoplasmic

yes

yes

N

33

0.16

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

10

20

UUP_ECOLI

P43672

uup

ycbH ycbI

b0949 JW0932

cytoplasmic peripheral IM protein, ABC transporter ATP-binding Uup

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (By similarity)

Homolog to import ATP-binding protein MalK.

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

Soluble IM Fraction

Cytoplasmic

yes

yes

N

27

0.126

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

10

YRAR_ECOLI

P45469

yraR

b3152 JW5528

cytoplasmic peripheral IM protein, Putative NAD(P)-binding YraR

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)

Experimentally detected by proteomics analysis of E.coli membrane fraction (Bernsel et al 2009). No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Potential peripherally associated protein facing the cytoplasm or integral inner membrane protein.

[19766000]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

Soluble IM Fraction

Cytoplasmic

yes

yes

N

32

0.195

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

9

12

PROQ_ECOLI

P45577

proQ

yebJ yobE yoeC

b1831 JW5300

cytoplasmic peripheral IM protein, RNA chaperone

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

[23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasm

Cytoplasmic

yes

yes

N

27

0.162

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

8

69

LUXS_ECOLI

P45578

luxS

ygaG

b2687 JW2662

cytoplasmic peripheral IM protein, S-ribosylhomocysteine lyase LuxS

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

Copurifies with trigger factor in pull down assays. Disulfide bond-containing protein [25664544]

[23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasmic

TF

no

yes

N

19

0.106

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

8

110

GSPE_ECOLI

P45759

gspE

yheG

b3326 JW3288

cytoplasmic peripheral IM protein, Putative type II secretion system E GspE

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (By similarity) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)

ATPase of the Type II secretion system (cryptic in K12) EspL (GspL homolog) protein interacts through its N-terminal cytoplasmic domain with EspL (GspL homolog) protein

[16525507] [17255937] [PMC398259]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasm

Cytoplasmic

yes

yes

N

23

0.111

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

9

21

YHDZ_ECOLI

P45769

yhdZ

b3271 JW3239

cytoplasmic peripheral IM protein, amino-acid ABC transporter ATP-binding YhdZ

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cell inner membrane

Cytoplasmic

yes

yes

N

12

0.122

CYT

0

-0.200913

-

0

0

N

i

CytoplasmicMembrane

9

11

YEDJ_ECOLI

P46144

yedJ

b1962 JW1945

cytoplasmic peripheral IM protein, YedJ

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Potential)

No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius.

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

IM (predicted-detected)

Cytoplasmic

no

yes

N

65

0.139

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

8

116

YHGF_ECOLI

P46837

yhgF

b3407 JW3370

cytoplasmic peripheral IM protein, YhgF

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Potential) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)

Experimentally detected by proteomics analysis of E.coli membrane fraction (Bernsel et al 2009). No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Potential peripherally associated protein facing the cytoplasm or integral inner membrane protein. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279]

[19766000] [23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

Soluble IM Fraction

Cytoplasmic

yes

yes

N

24

0.217

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

9

90

MLC_ECOLI

P50456

mlc

b1594 JW1586

cytoplasmic nucleoid peripheral IM protein, Mlc

Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (Experimental) Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (Probable)

Cytoplasmic global repressor of gene transcription that is recruited to the inner membrane where it binds the PtsB protein after the latter has been phosphorylated. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279]

[15255904] [23230279]

Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm

A, N, F1

IM (predicted)

Cytoplasmic

yes

yes

N

43

0.124

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

9

18

AEEP_ECOLI

P51981

ycjG

ycjH

b1325 JW1318

cytoplasmic peripheral IM protein (SEC through IM), L-Ala-D/L-Glu epimerase YcjG

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Potential)

No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius.

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

IM (predicted-detected)

Cytoplasmic

yes

yes

N

21

0.226

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

8

19

CSGD_ECOLI

P52106

csgD

b1040 JW1023

cytoplasmic nucleoid peripheral IM protein, CsgBAC operon transcriptional regulatory protein CsgD

Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (Experimental) Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (Potential)

Necessary for transcription of the csgBAC/ymdA operon. Plays a positive role in biofilm formation. CsgD despite its function as a transcription activator, is localized in the cytoplasmic membrane. LuxR-type HTH domain signature and profile. The luxR-type HTH domain is a DNA-binding, helix-turn-helix (HTH) domain of about 65 amino acids, present in transcription regulators of the LuxR/FixJ family of response regulators (PROSITE: PDOC0054).

[16513732] [20874755] [23138451]

Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm

A, N, F1

N/A

Cell inner membrane

Cytoplasmic

yes

yes

N

26

0.267

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

9

20

YFJP_ECOLI

P52131

yfjP

b2632 JW5419

cytoplasmic peripheral IM protein, YfjP

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)

Fluorescent fusions of YfjP have been monitored via fluorescence microscopy. YfjP located predominantly beside the membrane.

[17272300]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasmic

Prophage Integrase (EcoGene)

no

no

N

59

0.126

CYT

0

-0.200913

-

0

0

N

i

Unknown

2

35

FLIJ_ECOLI

P52613

fliJ

flaO flaS

b1942 JW1926

cytoplasmic peripheral IM protein, FliJ protein FliJ

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)

Part of FlgN-FlgK-FliJ-FlhA complex.

[22233518]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cell membrane; Peripheral membrane protein; Cytoplasmic side

Cytoplasmic

no

yes

N

36

0.121

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

10

89

WECF_ECOLI

P56258

wecF

rffT yifM

b4481 JW5596

cytoplasmic peripheral IM protein, TDP-N-acetylfucosamine:lipid II N-acetylfucosaminyltransferase WecF

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279]

[23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

IM (predicted)

Cell inner membrane

Cytoplasmic

pseudogene (Ochman & Davalos, 2006)

yes

yes

N

29

0.203

CYT

0

-0.200913

-

0

0

N

i

Unknown

2

48

in/in

SGCB_ECOLI

P58035

sgcB

b4565 JW5967

cytoplasmic peripheral IM protein, Putative phosphotransferase enzyme IIB component SgcB

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (By similarity)

Part of SgcABC complex, a putative PTSPermease. SgcA (Enzyme IIASgc)Is homologous to the IIA domains of the fructose - and mannitol-specific PTSPermeases (fruB and cmtB) which are peripherally accossiated proteins.

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasm

Cytoplasmic

no

yes

N

19

0.218

SpI

21

4.43024

-

0

0

Y

n4-11c19/20o

Cytoplasmic

9

MUKF_ECOLI

P60293

mukF

kicB

b0922 JW0905

cytoplasmic nucleoid peripheral IM protein, Chromosome partition MukF

Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (Experimental)

[16982609] [20921377] [23230279]

Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm

A, N, F1

N/A

Cytoplasm; nucleoid

Cytoplasmic

yes

yes

N

37

0.127

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

10

76

RSMH_ECOLI

P60390

rsmH

mraW yabC

b0082 JW0080

cytoplasmic peripheral IM protein, Ribosomal RNA small subunit methyltransferase H RsmH

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Potential) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)

Experimentally detected by proteomics analysis of E.coli membrane fraction (Bernsel et al 2009). No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Potential peripherally associated protein facing the cytoplasm or integral inner membrane protein. [10572301] Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279]

[10572301] [19766000] [23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

Soluble IM Fraction

Cytoplasm

Cytoplasmic

yes

yes

N

54

0.11

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

10

65

HIS1_ECOLI

P60757

hisG

b2019 JW2001

cytoplasmic peripheral IM protein, ATP phosphoribosyltransferase HisG

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

[23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasm

Cytoplasmic

yes

yes

N

44

0.181

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

10

64

LEPA_ECOLI

P60785

lepA

b2569 JW2553

cytoplasmic peripheral IM protein, Elongation factor 4 LepA

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

LepA has an amino-terminal domain of 112 residues that is homologous to a domain of similar size found in initiation factor 2, elongation factor Tu, and elongation factor G (IF2, EF-Tu, and EF-G). GTP-photoaffinity labeling and subcellular fractionation experiments indicate that LepA a membrane-bound GTP-binding protein [2999765]. GTPase, EF4/LepA involved in translational elongation, EF4 is stored at the membrane of Escherichia coli cells and released into the cytoplasm upon conditions of high ionic strength or low temperature where EF4 binds to stalled ribosomes and remobilizes them[21300907].Trigger factor-sensitive aggregation[19737520]. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279]

[15919657] [21300907] [23230279] [2999765]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

Soluble IM Fraction

Cell inner membrane

Cytoplasmic

TF

yes

yes

N

39

0.117

CYT

0

-0.200913

-

0

0

N

i

CytoplasmicMembrane

10

45

SYH_ECOLI

P60906

hisS

b2514 JW2498

cytoplasmic peripheral IM protein, Histidine--tRNA ligase HisS

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

[23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasm

Cytoplasmic

yes

yes

N

20

0.103

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

10

59

RISB_ECOLI

P61714

ribE

ribH ybaF

b0415 JW0405

cytoplasmic peripheral IM protein, 6,7-dimethyl-8-ribityllumazine synthase RibE

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

[23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasmic

yes

yes

N

69

0.129

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

9

MDH_ECOLI

P61889

mdh

b3236 JW3205

cytoplasmic peripheral IM protein, Malate dehydrogenase Mdh

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)

Trigger factor-sensitive aggregation. [7028159] Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279]

[15976458] [23230279] [7028159]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasmic (Experimental)

TF

yes

yes

N

18

0.251

SpI

17

0.386899

-

0

0

Y

n3-12c17/18o

Unknown

6

ISPG_ECOLI

P62620

ispG

gcpE

b2515 JW2499

cytoplasmic peripheral IM protein, 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase IspG

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

[23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasmic

yes

yes

N

44

0.118

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

9

GPMA_ECOLI

P62707

gpmA

gpm pgm pgmA

b0755 JW0738

cytoplasmic peripheral IM protein, 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase GpmA

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

[23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasmic

yes

yes

N

41

0.153

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

8

102

YAEH_ECOLI

P62768

yaeH

b0163 JW0159

cytoplasmic peripheral IM protein, UPF0325 YaeH

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

[23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

Soluble IM Fraction

Cytoplasmic

yes

yes

N

26

0.108

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

8

93

CLPB_ECOLI

P63284

clpB

htpM

b2592 JW2573

cytoplasmic peripheral IM protein, Chaperone ClpB

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

[23230279] [8376377]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasm

Cytoplasmic

yes

yes

N

22

0.119

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

10

75

MLAF_ECOLI

P63386

mlaF

yrbF

b3195 JW3162

cytoplasmic peripheral IM protein, Interphospholipid transport system ATP-binding MlaF

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

Phospholipid ABC transporter-binding protein ATP-binding proteins phospholipid ABC transporter-binding protein ATP-binding protein. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279]

[23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

Soluble IM Fraction

Cell inner membrane

Cytoplasmic

yes

yes

N

64

0.109

CYT

0

-0.200913

-

0

0

N

i

CytoplasmicMembrane

10

23

YHES_ECOLI

P63389

yheS

b3352 JW3315

cytoplasmic peripheral IM protein, ABC transporter ATP-binding YheS

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Potential)

Phospholipid ABC transporter-binding protein ATP-binding proteins phospholipid ABC transporter-binding protein ATP-binding proteins Trigger factor-sensitive aggregation.

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasmic

TF

yes

yes

N

54

0.115

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

9

39

MLAB_ECOLI

P64602

mlaB

yrbB

b3191 JW5535

cytoplasmic peripheral IM protein, Interphospholipid transport system binding MlaB

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (By similarity) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

Probable phospholipid ABC transporter-binding protein mlaB Probable phospholipid ABC transporter-binding protein mlaB Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279]

[23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

Soluble IM Fraction

Cytoplasm

Cytoplasmic

no

yes

N

66

0.108

CYT

0

-0.200913

-

0

0

N

i

Unknown

2

HLDD_ECOLI

P67910

hldD

htrM rfaD waaD

b3619 JW3594

cytoplasmic peripheral IM protein, ADP-L-glycero-D-manno-heptose-6-epimerase HldD

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

[2198271] [23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasmic (Experimental)

yes

yes

N

19

0.195

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

9

26

GRCA_ECOLI

P68066

grcA

yfiD

b2579 JW2563

cytoplasmic peripheral IM protein, Autonomous glycyl radical cofactor GrcA

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

[23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasmic

yes

yes

N

30

0.139

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

9

MALK_ECOLI

P68187

malK

b4035 JW3995

cytoplasmic peripheral IM protein, Maltose/maltodextrin import ATP-binding MalK

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)

ATPase of maltose uptake system. MalF functions in maltose transport as a 1:1:2 complex with the integral inner membrane protein MalG and the peripheral inner membrane protein MalK Crystal structure of a catalytic intermediate of the maltose transporter. [18033289]

[16079137] [18456666]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

Soluble IM Fraction

Cell inner membrane

Cytoplasmic

yes

yes

N

15

0.106

CYT

0

-0.200913

-

0

0

N

i

CytoplasmicMembrane

10

19

SRA_ECOLI

P68191

sra

rpsV

b1480 JW5962

cytoplasmic peripheral IM protein, Stationary-phase-induced ribosome-associated Sra

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)

Small protein identified experimentally[19121005]. Stationary-phase-induced and ribosome-associated protein[11292794]. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry)[23230279].

[11292794] [23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasm

Cytoplasmic

no

yes

N

26

0.1

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

10

FIXC_ECOLI

P68644

fixC

yaaS

b0043 JW0042

cytoplasmic peripheral IM protein, FixC

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (By similarity)

The protein has hydrophobic patches all over it's sequence. Could be part of an electron transfer system required for anaerobic carnitine reduction so this is consistent with a membrane association. Strong Prediction on both Phobius and TMHMM for an aminoterminal weakly hydrophobic region. Other studies propose that this sequence may be an aminoterminal signal peptide without evidence for cleavage. The fixABCX genes in Rhodospirillum Rubrum encode a putative membrane complex participating in electron transfer to nitrogenase where FixA and B are soluble proteins.However the RrFixC expressed in E.coli was localized at the membrane fraction and was not carbonate extracted suggesting that it is an integral membrane protein. From another study in Rhizobia. Primary sequence homology, the FixC family is a highly conserved flavoprotein distantly related to the mitochondrial ETF-QO (peripheral, membrane-associated ETF : ubiquinone oxidoreductase) yet lacking both hydrophobic and amphipathic transmembrane {alpha}-helices. Thus FixC family is unlikely to be stably membrane-associated.

[14702404] [15028689] [7473063]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Periplasmic

yes

yes

N

26

0.179

CYT

0

-0.200913

-

0

0

Y

n8-19c25/26o

Cytoplasmic

8

35

AMPA_ECOLI

P68767

pepA

carP xerB

b4260 JW4217

cytoplasmic peripheral IM protein, Cytosol aminopeptidase PepA

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

[23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasmic

yes

yes

N

35

0.112

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

9

58

KAD_ECOLI

P69441

adk

dnaW plsA

b0474 JW0463

cytoplasmic peripheral IM protein, Adenylate kinase Adk

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

Adk cell localization has been monitored via fluorescence microscopy after protein was chromosomally tagged with fluorescent protein/s. These experiments indicate that Adk is distributed diffusely throughout cytoplasm. [8650159]

[17272300] [23230279] [8650159]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

Soluble IM Fraction

Cytoplasm

Cytoplasmic (Experimental)

yes

yes

N

18

0.12

CYT

0

-0.200913

-

0

0

Y

n3-12c17/18o

Cytoplasmic

10

104

LCFA_ECOLI

P69451

fadD

oldD

b1805 JW1794

cytoplasmic peripheral IM protein, Long-chain-fatty-acid--CoA ligase FadD

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

Acyl-coenzyme A (CoA) synthetase that may interact with the inner membrane. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279]

[23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cell membrane

Cytoplasmic

yes

yes

N

63

0.12

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

9

36

YTFE_ECOLI

P69506

ytfE

b4209 JW4167

cytoplasmic peripheral IM protein, Iron-sulfur cluster repair YtfE

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)

Experimentally detected by proteomics analysis of E.coli membrane fraction (Bernsel et al 2009). No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Potential peripherally associated protein facing the cytoplasm or integral inner membrane protein.

[19766000]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

Soluble IM Fraction

Cytoplasm

Cytoplasmic

yes

yes

N

21

0.13

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

10

103

PTGA_ECOLI

P69783

crr

gsr iex tgs treD

b2417 JW2410

cytoplasmic peripheral IM protein, PTS system glucose-specific EIIA component Crr

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279]

[12717030] [23230279] [2960675]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasm

Cytoplasmic (Experimental)

yes

yes

N

44

0.106

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

10

113

PTQA_ECOLI

P69791

chbA

celC

b1736 JW1725

cytoplasmic peripheral IM protein, PTS system N,N'-diacetylchitobiose-specific EIIA component ChbA

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)

IIA component of the phosphoenolpyruvate (glycose transferase PTS system) which is a prototypic signaling system responsible for the vectorial uptake and phosphorylation of carbohydrate substrates. Unlike most PTS systems, phosphoenolpyruvate is composed of two soluble proteins, IIA and IIB, and one transmembrane receptor IIC.

[10913122]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasm

Cytoplasmic

pseudogene (Ochman & Davalos, 2006)

yes

yes

N

38

0.187

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

10

111

PTQB_ECOLI

P69795

chbB

celA

b1738 JW1727

cytoplasmic peripheral IM protein, PTS system N,N'-diacetylchitobiose-specific EIIB component ChbB

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)

IIB component of the phosphoenolpyruvate (glycose transferase PTS system) which is a prototypic signaling system responsible for the vectorial uptake and phosphorylation of carbohydrate substrates. Unlike most PTS systems, phosphoenolpyruvate is composed of two soluble proteins, IIA and IIB, and one transmembrane receptor IIC. [2092358]

[10913122] [2092358]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasm

Membrane associated (Experimental)

yes

yes

N

28

0.176

CYT

0

-0.200913

-

0

0

Y

n8-19c27/28o

Cytoplasmic

10

97

PTNAB_ECOLI

P69797

manX

gptB ptsL

b1817 JW1806

cytoplasmic peripheral IM protein, PTS system mannose-specific EIIAB component ManX

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)

N,N'-diacetylchitobiose-specific phosphotransferase enzyme IIB component. Identified by native-PAGE technique for the study of membrane protein complexes in the inner and outer membranes of Escherichia coli. The mannose phosphotransferase system transporter (EIIman) is composed of three subunits, ManX (IIABman), ManY (IICman), and ManZ (IIDman), which are reported to assemble in a 2:1:2 stoichiometry. The IIC and IID components are integral inner membrane proteins. Identified in the inner membrane by biochemical fractionation followed by two-dimensional gel electrophoresis and tandem mass spectrometry. [15911532] [10094700] Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279]

[10094700] [15911532] [15919657] [16079137] [23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

Soluble IM Fraction

Cytoplasm

Cytoplasmic (Experimental)

no

yes

N

43

0.126

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

10

93

PTFAH_ECOLI

P69811

fruB

fpr fruF

b2169 JW2156

cytoplasmic peripheral IM protein, Multiphosphoryl transfer FruB

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)

Identified in the inner membrane by biochemical fractionation followed by two-dimensional gel electrophoresis and tandem mass spectrometry. [15911532] [8013873] [2841594]

[15911532] [2841594] [8013873]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

Soluble IM Fraction

Cytoplasm

Cytoplasmic (Experimental)

no

yes

N

39

0.108

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

10

91

PTFB2_ECOLI

P69816

frwB

yijK

b3950 JW3922

cytoplasmic peripheral IM protein, PTS system fructose-like EIIB component 2 FrwB

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (By similarity)

The sgaTBA genes of Escherichia coli encode a putative 12-transmembrane alpha-helical segment (12 TMS) transporter, an enzyme IIB-like protein and an enzyme IIA-like protein of the phosphotransferase system (PTS), respectively. SgaT represents a novel prototypical enzyme IIC that functions with SgaA and SgaB to allow phosphoryl transfer from HPr(his-P) to l-ascorbate via the phosphoryl transfer pathway

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasm

Cytoplasmic

yes

yes

N

21

0.363

SpI

20

4.62655

-

0

0

N

i

Cytoplasmic

10

87

ULAC_ECOLI

P69820

ulaC

ptxA sgaA yjfU

b4195 JW4153

cytoplasmic peripheral IM protein, Ascorbate-specific PTS system EIIA component UlaC

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Potential)

[12644495]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasm

Cytoplasmic

no

yes

N

47

0.126

CYT

0

-0.200913

-

0

0

N

i

CytoplasmicMembrane

7

84

ULAB_ECOLI

P69822

ulaB

sgaB yjfT

b4194 JW4152

cytoplasmic peripheral IM protein, Ascorbate-specific PTS system EIIB component UlaB

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (By similarity)

The sgaTBA genes of Escherichia coli encode a putative 12-transmembrane alpha-helical segment (12 TMS) transporter, an enzyme IIB-like protein and an enzyme IIA-like protein of the phosphotransferase system (PTS), respectively[12644495]. All three proteins are required for the anaerobic utilization and uptake of L-ascorbate in vivo and its phosphoenolpyruvate-dependent phosphorylation in vitro[12644495].

[12644495]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasm

Cytoplasmic

no

yes

N

62

0.14

CYT

0

-0.200913

-

0

0

N

i

Unknown

2

53

PTMA_ECOLI

P69824

cmtB

b2934 JW2901

cytoplasmic peripheral IM protein, Mannitol-specific cryptic phosphotransferase enzyme IIA component CmtB

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (By similarity) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)

[9525892]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasm

Cytoplasmic

no

yes

N

19

0.136

CYT

0

-0.200913

-

0

0

N

i

Unknown

2

96

PTKA_ECOLI

P69828

gatA

b2094 JW2078/JW2081

cytoplasmic peripheral IM protein, PTS system galactitol-specific EIIA component GatA

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)

Part of phosphoenolpyruvate-dependent carbohydrate transport system (PTS). The carbohydrate-specific permease, is constituted of two cytoplasmic domains, IIA and IIB, and a transmembrane channel IIC domain. GatA (IIA domain) interacts with GatB (IIB domain) [16963640] . Copurifies with trigger factor in pull down assays [19737520].

[16963640]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasm

Cytoplasmic

TF

no

yes

N

69

0.109

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

10

71

PTSN_ECOLI

P69829

ptsN

rpoP yhbI

b3204 JW3171

cytoplasmic peripheral IM protein, Nitrogen regulatory PtsN

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)

enzyme IIA(Ntr) of the nitrogen PTS regulates the intracellular concentration of K(+) by direct interaction with TrkA and KdpD. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279]

[17289841] [21605551] [23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasm

Cytoplasmic

yes

yes

N

26

0.129

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

10

82

DMSD_ECOLI

P69853

dmsD

ynfI

b1591 JW5262

cytoplasmic peripheral IM protein, Tat proofreading chaperone DmsD

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)

Chaperone for TAT signal peptides The ynfEFGHI operon is a paralogue of the Escherichia coli dmsABC operon. ynfE and ynfF are paralogues of dmsA. ynfG and ynfH are paralogues of dmsB and dmsC, respectively. [14522592] DmsD is a redox enzyme maturation protein (REMP) which interacts with the twin-arginine leader sequence of DmsA, the catalytic subunit of DMSO reductase. It interacts with several general chaperones (DnaK, DnaJ, GrpE, GroEL, Tig and Ef-Tu) via in vitro protein-protein interaction screening approaches. It was also found to interact with proteins involved in the molybdenum cofactor biosynthesis pathway. [20153451]

[12813051] [19652330] [20153451]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cell inner membrane

Cytoplasmic

yes

yes

N

31

0.453

SpI

30

2.43679

-

0

0

N

i

CytoplasmicMembrane

10

POTA_ECOLI

P69874

potA

b1126 JW1112

cytoplasmic peripheral IM protein, Spermidine/putrescine import ATP-binding PotA

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)

Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279]

[11976340] [1939142] [23230279] [7592703] [8366082]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

Soluble IM Fraction

Cell inner membrane

Cytoplasmic (Experimental)

yes

yes

N

22

0.124

CYT

0

-0.200913

-

0

0

N

i

CytoplasmicMembrane

9

93

FCTA_ECOLI

P69902

frc

yfdW

b2374 JW2371

cytoplasmic peripheral IM protein, Formyl-CoA:oxalate CoA-transferase Frc

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Potential)

Hypothesized to interact with the oxalate/formate antiporter and to oxalyl-CoA decarboxylase to form a large complex. 3D structure formyl-CoA transferase in Oxalobacter formigenes reveals a CoA binding site at the interface of an interlocked dimer[12839984].

[12839984]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

IM (predicted)

Cytoplasmic

yes

yes

N

24

0.119

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

9

81

DCEB_ECOLI

P69910

gadB

b1493 JW1488

cytoplasmic peripheral IM protein, Glutamate decarboxylase beta GadB

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)

Localized exclusively in the cytoplasm at neutral pH, but is recruited to the membrane when the pH falls.

[12912902]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasm

Cytoplasmic

yes

yes

N

28

0.112

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

10

14

HDA_ECOLI

P69931

hda

idaB yfgE

b2496 JW5397

cytoplasmic nucleoid peripheral IM protein, DnaA regulatory inactivator Hda

Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (Probable)

Homolgous to DnaA and more specific to C-terminal anchor of DnaA.

[12618445]

Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm

A, N, F1

Soluble IM Fraction

Cell inner membrane

Cytoplasmic

yes

yes

N

33

0.157

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

9

YAAY_ECOLI

P75620

yaaY

b0024 JW5003

cytoplasmic peripheral IM protein, YaaY

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Potential)

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Membrane anchored

no

yes

N

43

0.125

CYT

0

-0.200913

-

1

0

N

i

CytoplasmicMembrane

9

YAFS_ECOLI

P75672

yafS

b0213 JW0203

cytoplasmic peripheral IM protein, YafS

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

[23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasmic

yes

yes

N

54

0.121

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

8

YAHK_ECOLI

P75691

yahK

b0325 JW0317

cytoplasmic peripheral IM protein, Aldehyde reductase YahK

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Potential)

No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279] Disulfide bond-containing protein [25664544]

[23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

IM (predicted-detected)

Cytoplasmic

no

yes

N

53

0.148

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

9

73

GSIA_ECOLI

P75796

gsiA

yliA

b0829 JW5897

cytoplasmic peripheral IM protein, Glutathione import ATP-binding GsiA

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279]

[23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

Soluble IM Fraction

Cell inner membrane

Cytoplasmic

yes

yes

N

62

0.122

CYT

0

-0.200913

-

0

0

N

i

CytoplasmicMembrane

9

YBJS_ECOLI

P75821

ybjS

b0868 JW5819

cytoplasmic peripheral IM protein, YbjS

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

[23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasmic

no

yes

N

30

0.118

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

8

6

YBJX_ECOLI

P75829

ybjX

b0877 JW0861

cytoplasmic peripheral IM protein, YbjX

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Potential) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)

In Shigella the homologous protein is required for the expression, or correct membrane localisation of IcsA (VirG) on the bacterial cell surface.In Pasteurella haemolytica lapB (P32181), which is thought to be membrane-associated. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279]

[19766000] [23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

Soluble IM Fraction

Cytoplasmic

yes

yes

N

20

0.111

CYT

0

-0.200913

-

0

0

N

i

Unknown

2

21

LONH_ECOLI

P75867

ycbZ

b0955 JW0938

cytoplasmic peripheral IM protein, Putative Lon protease homolog YcbZ

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

[23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasmic

yes

yes

N

24

0.113

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

9

RLMI_ECOLI

P75876

rlmI

yccW

b0967 JW5898

cytoplasmic peripheral IM protein, Ribosomal RNA large subunit methyltransferase I RlmI

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

[23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasm

Cytoplasmic

yes

yes

N

29

0.117

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

9

YCDY_ECOLI

P75915

ycdY

b1035 JW1018

cytoplasmic peripheral IM protein, Chaperone YcdY

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

[23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasmic

yes

yes

N

38

0.184

CYT

0

-0.200913

-

0

0

N

i

CytoplasmicMembrane

9

89

THIK_ECOLI

P75948

thiK

ycfN

b1106 JW1092

cytoplasmic peripheral IM protein, Thiamine kinase ThiK

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

IM (predicted)

Cytoplasmic

yes

yes

N

39

0.11

CYT

0

-0.200913

-

0

0

N

i

Unknown

2

9

LOLD_ECOLI

P75957

lolD

ycfV

b1117 JW5162

cytoplasmic peripheral IM protein, -releasing system ATP-binding LolD

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

[23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

Soluble IM Fraction

Cell inner membrane; Peripheral membrane protein

Cytoplasmic

yes

yes

N

65

0.122

CYT

0

-0.200913

-

0

0

N

i

CytoplasmicMembrane

10

25

YMFJ_ECOLI

P75973

ymfJ

b1144 JW1130

cytoplasmic peripheral IM protein, YmfJ

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)

Experimentally detected by proteomics analysis of E.coli membrane fraction (Bernsel et al 2009). No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Potential peripherally associated protein facing the cytoplasm or integral inner membrane protein.

[19766000]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

Soluble IM Fraction

Cytoplasmic

Prophage Integrase (EcoGene)

no

no

N

48

0.139

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

8

47

YCGR_ECOLI

P76010

ycgR

b1194 JW1183

Flagellar cytoplasmic peripheral IM protein, brake YcgR

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)

Acts as a "backstop brake" to control flagellar motor direction and speed to affect chemotaxis.

[20303158] [20346719]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Bacterial flagellum basal body

Cytoplasmic

flagellum

no

yes

N

60

0.155

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

8

38

OPPD_ECOLI

P76027

oppD

b1246 JW1238

cytoplasmic peripheral IM protein, Oligopeptide transport ATP-binding OppD

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (By similarity) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

Oligopeptide permease (Opp) transporters are located in the plasma membrane and have functions generally related to the uptake of peptides from the extracellular environment. OppD is the ATP-binding protein. [24793159] Based on sequence similarity, OppB and OppC are the membrane components of the ABC transporter, and OppD and OppF are the ATP-binding components of the ABC transporter [1738314] Opp-LacZ hybrid proteins were used to show that OppB, OppC and OppD are membrane-associated proteins. [2821267] [16045610] [22087229 ] [3926486] Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279]

[16045610] [1738314] [22087229] [23230279] [24793159] [2821267] [3926486]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

Soluble IM Fraction

Cell inner membrane; Peripheral membrane protein

Cytoplasmic (Experimental)

yes

yes

N

69

0.132

CYT

0

-0.200913

-

0

0

N

i

CytoplasmicMembrane

10

18

YCJR_ECOLI

P76044

ycjR

b1314 JW5202

cytoplasmic peripheral IM protein, Probable ketose 3-epimerase YcjR

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)

Experimentally detected by proteomics analysis of E.coli membrane fraction (Bernsel et al 2009). No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Potential peripherally associated protein facing the cytoplasm or integral inner membrane protein.

[19766000]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

Soluble IM Fraction

Cytoplasmic

no

yes

N

47

0.118

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

8

44

INSH5_ECOLI

P76071

insH5

b1370 JW1363

cytoplasmic peripheral IM protein, Transposase InsH for insertion sequence element IS5Y

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)

Experimentally detected by proteomics analysis of E.coli membrane fraction (Bernsel et al 2009). No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Potential peripherally associated protein facing the cytoplasm or integral inner membrane protein.

[19766000]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

Soluble IM Fraction

Cytoplasmic

Insertion Sequence, Transposase, pseudogene (Uniprot)

no

no

N

49

0.125

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

8

PAAC_ECOLI

P76079

paaC

ydbP

b1390 JW1385

cytoplasmic peripheral IM protein, 1,2-phenylacetyl-CoA epoxidase, subunit C PaaC

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)

Experimentally detected by proteomics analysis of E.coli membrane fraction (Bernsel et al 2009). No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Potential peripherally associated protein facing the cytoplasm or integral inner membrane protein.

[19766000]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

Soluble IM Fraction

Cytoplasmic

no

yes

N

57

0.144

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

8

SUFE_ECOLI

P76194

sufE

ynhA

b1679 JW1669

cytoplasmic peripheral IM protein, Cysteine desulfuration SufE

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)

SufE protein and the SufBCD complex stimulate the sulfurylase activity of SufS. Probably through direct interaction .

[12941942]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasm

Cytoplasmic

yes

yes

N

66

0.103

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

9

104

MTFA_ECOLI

P76346

mtfA

yeeI

b1976 JW1958

cytoplasmic peripheral IM protein, MtfA

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)

Cytoplasmic transciption factor that shuttles from chromosome to the membranes as a peripheral protein on the PtsG transporter. Specifically it binds to the unphosphorylated, trnasport-mediating form of the EIIBCGlc subunit. [16855233]

[15063852] [16855233]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasm

Cytoplasmic

yes

yes

N

36

0.109

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

8

87

YEEL_ECOLI

P76349

yeeL

b4497 b1979/b1980

cytoplasmic peripheral IM protein, Putative YeeL

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)

Experimentally detected by proteomics analysis of E.coli membrane fraction (Bernsel et al 2009). No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Potential peripherally associated protein facing the cytoplasm or integral inner membrane protein.

[19766000]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

Soluble IM Fraction

Cytoplasmic

pseudogene (Uniprot), pseudogene (Ochman & Davalos, 2006)

no

no

N

19

0.131

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

8

13

ARND_ECOLI

P76472

arnD

pmrJ yfbH

b2256 JW2250

cytoplasmic peripheral IM protein, Probable 4-deoxy-4-formamido-L-arabinose-phosphoundecaprenol deformylase ArnD

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

[23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasmic

yes

yes

N

67

0.132

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

9

10

SIXA_ECOLI

P76502

sixA

yfcW

b2340 JW2337

cytoplasmic peripheral IM protein, Phosphohistidine phosphatase SixA

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)

Exhibits phosphohistidine phosphatase activity towards the HPt domain of the ArcB sensor involved in the multistep His-Asp phosphorelay[9489669]. The structure of SixA reveals it forms a relatively shallow groove important for accommodating the kidney-shaped four-helix bundle of the HPt domain of AcrB[17628145].

[10734219] [17628145] [9489669]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

IM (predicted)

Cytoplasmic

yes

yes

N

57

0.122

CYT

0

-0.200913

-

0

0

N

i

Unknown

2

79

FADI_ECOLI

P76503

fadI

yfcY

b2342 JW2339

cytoplasmic peripheral IM protein, 3-ketoacyl-CoA thiolase FadI

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

[23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasm

Cytoplasmic

yes

yes

N

32

0.192

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

9

9

YFFO_ECOLI

P76546

yffO

b2446

cytoplasmic peripheral IM protein, YffO

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)

Experimentally detected by proteomics analysis of E.coli membrane fraction (Bernsel et al 2009). No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Potential peripherally associated protein facing the cytoplasm or integral inner membrane protein.

[19766000]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

Soluble IM Fraction

Cytoplasmic

Prophage Integrase (EcoGene)

no

no

N

61

0.105

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

8

YFFR_ECOLI

P76549

yffR

b2449

cytoplasmic peripheral IM protein, YffR

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)

Experimentally detected by proteomics analysis of E.coli membrane fraction (Bernsel et al 2009). No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Potential peripherally associated protein facing the cytoplasm or integral inner membrane protein.

[19766000]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

Soluble IM Fraction

Cytoplasmic

Prophage Integrase (EcoGene)

no

no

N

20

0.169

CYT

0

-0.200913

-

0

0

Y

n3-14c19/20o

Unknown

2

MAO2_ECOLI

P76558

maeB

ypfF

b2463 JW2447

cytoplasmic peripheral IM protein, NADP-dependent malic enzyme MaeB

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

Trigger factor-sensitive aggregation.

[23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasmic

TF

yes

yes

N

45

0.165

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

9

81

YGEH_ECOLI

P76639

ygeH

b2852 JW2820

cytoplasmic peripheral IM protein, YgeH

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)

Fluorescent fusions of YgeH have been monitored via fluorescence microscopy. YgeH located predominantly beside the membrane.

[17272300]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasmic

no

yes

N

53

0.108

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

8

19

YNJD_ECOLI

P76909

ynjD

b1756 JW5286

cytoplasmic peripheral IM protein, ABC transporter ATP-binding YnjD

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (By similarity)

Homolog to import ATP-binding protein MalK. Homolog to import ATP-binding protein MalK.

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasmic

yes

yes

N

53

0.159

CYT

0

-0.200913

-

0

0

N

i

CytoplasmicMembrane

9

19

YLBE_ECOLI

P77129

ylbE

b4572 b0519

cytoplasmic peripheral IM protein, YlbE

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)

Experimentally detected by proteomics analysis of E.coli membrane fraction (Bernsel et al 2009). No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Potential peripherally associated protein facing the cytoplasm or integral inner membrane protein.

[19766000]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

Soluble IM Fraction

Cytoplasmic

pseudogene (Ochman & Davalos, 2006)

no

yes

N

46

0.139

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

8

33

YAHF_ECOLI

P77187

yahF

b0320 JW0312

cytoplasmic peripheral IM protein, YahF

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)

Experimentally detected by proteomics analysis of E.coli membrane fraction (Bernsel et al 2009). No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Potential peripherally associated protein facing the cytoplasm or integral inner membrane protein.

[19766000]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

Soluble IM Fraction

Cytoplasmic

yes

yes

N

28

0.139

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

8

RSXB_ECOLI

P77223

rsxB

rnfB ydgM

b1628 JW1620

cytoplasmic peripheral IM protein, Ion-translocating oxidoreductase complex subunit B RsxB

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

Electron transport. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279]

[23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cell inner membrane

Periplasmic

yes

yes

N

31

0.278

SpI

30

4.42273

-

1

0

Y

n3-14c19/20o

CytoplasmicMembrane

10

22

LSRA_ECOLI

P77257

lsrA

ego ydeX

b1513 JW1506

cytoplasmic peripheral IM protein, Autoinducer 2 import ATP-binding LsrA

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cell inner membrane

Cytoplasmic

no

yes

N

42

0.176

CYT

0

-0.200913

-

0

0

N

i

CytoplasmicMembrane

10

17

DDPD_ECOLI

P77268

ddpD

yddP

b1484 JW1479

cytoplasmic peripheral IM protein, Probable D,D-dipeptide transport ATP-binding DdpD

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cell inner membrane

Cytoplasmic

yes

yes

N

25

0.176

CYT

0

-0.200913

-

0

0

N

i

CytoplasmicMembrane

7

23

FETA_ECOLI

P77279

fetA

ybbL

b0490 JW0479

cytoplasmic peripheral IM protein, Probable iron export ATP-binding FetA

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (By similarity)

Homolog to import ATP-binding protein MalK.

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cell inner membrane

Cytoplasmic

yes

yes

N

33

0.126

CYT

0

-0.200913

-

0

0

N

i

CytoplasmicMembrane

9

42

YDJJ_ECOLI

P77280

ydjJ

b1774 JW1763

cytoplasmic peripheral IM protein, zinc-type alcohol dehydrogenase-like YdjJ

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Potential)

No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius.

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

IM (predicted-detected)

Cytoplasmic

no

yes

N

46

0.11

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

9

77

HYFG_ECOLI

P77329

hyfG

b2487 JW2472

cytoplasmic peripheral IM protein, Hydrogenase-4 component G HyfG

Phobius predicts a slight hydrophobic area within the body protein

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

IM (predicted)

Cytoplasmic

yes

yes

N

27

0.133

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

9

ARNA_ECOLI

P77398

arnA

pmrI yfbG

b2255 JW2249

cytoplasmic peripheral IM protein, Bifunctional polymyxin resistance ArnA

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

[23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasmic

no

yes

N

59

0.178

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

9

3

FADJ_ECOLI

P77399

fadJ

yfcX

b2341 JW2338

cytoplasmic peripheral IM protein, Fatty acid oxidation complex subunit alpha FadJ

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

[23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasm

Cytoplasmic

yes

yes

N

70

0.113

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

9

15

PTFX1_ECOLI

P77439

fryA

ypdD

b2383 JW2380

cytoplasmic peripheral IM protein, Multiphosphoryl transfer 1 FryA

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Potential)

Multiphosphoryl transfer protein 1

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasm

Cytoplasmic

no

yes

N

17

0.156

CYT

0

-0.200913

-

0

0

Y

n2-10c16/17o

Cytoplasmic

9

38

SUFS_ECOLI

P77444

sufS

csdB ynhB

b1680 JW1670

cytoplasmic peripheral IM protein, Cysteine desulfurase SufS

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)

SufE protein and the SufBCD complex stimulate the sulfurylase activity of SufS probably through direct interaction.

[12941942]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasm

Cytoplasmic

yes

yes

N

66

0.18

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

10

11

YCJV_ECOLI

P77481

ycjV

ymjB

b4524 JW1311/JW5203 b1318

cytoplasmic peripheral IM protein, Putative ABC transporter ATP-binding YcjV

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (By similarity)

Homolog to import ATP-binding protein MalK.

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasmic

pseudogene (Uniprot)

yes

no

N

37

0.103

CYT

0

-0.200913

-

0

0

N

i

CytoplasmicMembrane

9

10

DXS_ECOLI

P77488

dxs

yajP

b0420 JW0410

cytoplasmic peripheral IM protein, 1-deoxy-D-xylulose-5-phosphate synthase Dxs

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

YeaA has been viewed through fluorescence microscopy and located evenly throughout cytoplasm. Small scale study of labeling chromosomal coding DNA sequences (CDSs) with fluorescent protein genes. [17272300]

[17272300] [23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasmic

yes

yes

N

52

0.114

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

9

21

PRPE_ECOLI

P77495

prpE

yahU

b0335 JW0326

cytoplasmic peripheral IM protein, Propionate--CoA ligase PrpE

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (By similarity)

Homolog to FadD13 protein of Mycobacterium that is peripheral[22560731].

[22560731]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasmic

no

yes

N

44

0.132

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

9

13

SUFC_ECOLI

P77499

sufC

ynhD

b1682 JW1672

cytoplasmic peripheral IM protein, Probable ATP-dependent transporter SufC

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (By similarity) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

SufE protein and the SufBCD complex stimulate the sulfurylase activity of SufS. Probably through direct interaction. Homolog to import ATP-binding protein MalK. [12554644]

[12554644] [12941942]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasm

Cytoplasmic

yes

yes

N

32

0.213

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

10

69

YPHE_ECOLI

P77509

yphE

b2547 JW2531

cytoplasmic peripheral IM protein, ABC transporter ATP-binding YphE

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (By similarity)

Homolog to import ATP-binding protein MalK. Homolog to import ATP-binding protein MalK.

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasmic

yes

yes

N

26

0.136

CYT

0

-0.200913

-

0

0

N

i

CytoplasmicMembrane

7

5

SUFB_ECOLI

P77522

sufB

ynhE

b1683 JW5273

cytoplasmic peripheral IM protein, FeS cluster assembly SufB

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)

Completely soluble no evidence for hyrophobic patches. IM binding determined by immunolocalization.

[11943156]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasmic

yes

yes

N

31

0.117

CYT

0

-0.200913

-

0

0

N

i

Unknown

2

43

RSXC_ECOLI

P77611

rsxC

rnfC ydgN

b1629 JW1621

cytoplasmic peripheral IM protein, Ion-translocating oxidoreductase complex subunit C RsxC

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279]

[23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cell inner membrane

Cytoplasmic

yes

yes

N

54

0.101

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

8

DDPF_ECOLI

P77622

ddpF

yddO

b1483 JW1478

cytoplasmic peripheral IM protein, Probable D,D-dipeptide transport ATP-binding DdpF

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cell inner membrane

Cytoplasmic

yes

yes

N

32

0.115

CYT

0

-0.200913

-

0

0

N

i

CytoplasmicMembrane

9

18

ARCM_ECOLI

P77624

yahI

b0323 JW0315

cytoplasmic peripheral IM protein, Carbamate kinase-like YahI

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)

Experimentally detected by proteomics analysis of E.coli membrane fraction (Bernsel et al 2009). No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Potential peripherally associated protein facing the cytoplasm or integral inner membrane protein.

[19766000]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

Soluble IM Fraction

Cytoplasmic

yes

yes

N

20

0.12

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

9

82

SUFA_ECOLI

P77667

sufA

ydiC

b1684 JW1674

cytoplasmic peripheral IM protein, SufA

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Potential)

[19366265]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasmic

yes

no

N

34

0.137

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

10

85

SUFD_ECOLI

P77689

sufD

ynhC

b1681 JW1671

cytoplasmic peripheral IM protein, FeS cluster assembly SufD

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)

Forms complex with SufC and SufB.

[10322040]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasmic

yes

yes

N

67

0.127

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

8

36

OPPF_ECOLI

P77737

oppF

b1247 JW1239

cytoplasmic peripheral IM protein, Oligopeptide transport ATP-binding OppF

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279]

[23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

Soluble IM Fraction

Cell inner membrane; Peripheral membrane protein

Cytoplasmic

yes

yes

N

70

0.124

CYT

0

-0.200913

-

0

0

N

i

CytoplasmicMembrane

10

17

YDIJ_ECOLI

P77748

ydiJ

b1687 JW1677

cytoplasmic peripheral IM protein, YdiJ

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

[15919657] [23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

Soluble IM Fraction

Cytoplasmic

yes

yes

N

29

0.119

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

9

2

YDCT_ECOLI

P77795

ydcT

b1441 JW1436

cytoplasmic peripheral IM protein, ABC transporter ATP-binding YdcT

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (By similarity)

Homolog to import ATP-binding protein MalK.

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasmic

yes

yes

N

20

0.146

CYT

0

-0.200913

-

0

0

N

i

CytoplasmicMembrane

10

26

YGHU_ECOLI

Q46845

yghU

b2989 JW5492

cytoplasmic peripheral IM protein, Disulfide-bond oxidoreductase YghU

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Potential)

Phobius gives a large hydrophobic stretch that is not too hydrophobic to be a real TM. hydropathy profile also shows hydrophobic strecthes. localization peripheral inner membrane protein possibly also integral inner membrane/phobius gives a large hydrophobic stretch that is not too hydrophobic to be a real TM. hydropathy profile also shows hydrophobic strecthes. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). [23230279]

[23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Membrane anchored

yes

yes

N

24

0.332

CYT

0

-0.200913

-

1

0

N

i

Cytoplasmic

9

49

YQHD_ECOLI

Q46856

yqhD

b3011 JW2978

cytoplasmic peripheral IM protein, Alcohol dehydrogenase YqhD

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)

[23230279]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasmic

yes

yes

N

21

0.138

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

9

91

CSDA_ECOLI

Q46925

csdA

ygdJ

b2810 JW2781

cytoplasmic peripheral IM protein, Cysteine desulfurase CsdA

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)

Assembly cofactor of ribosome; associate with the 50S subunit. Under cold-shock conditions CsdA participates as an alternative DEAD-box RNA helicase together with RNase E in a ???cold shock??? RNA degradosome (Prud???homme-Genereux et al., 2004). It is therefore possible that CsdA may associate with the inner membrane via RNAseE.

[15554978] [21529161]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasmic

yes

yes

N

59

0.194

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

9

9

FDRA_ECOLI

Q47208

fdrA

ylbD

b0518 JW0506

cytoplasmic peripheral IM protein, FdrA

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)

[7500942]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cell membrane

Integral Membrane Protein

yes

yes

N

31

0.11

CYT

0

-0.200913

-

0

0

N

i

Unknown

2

56

TAUB_ECOLI

Q47538

tauB

ssiB yaiQ

b0366 JW0358

cytoplasmic peripheral IM protein, Taurine import ATP-binding TauB

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cell inner membrane

Cytoplasmic

yes

yes

N

57

0.124

CYT

0

-0.200913

-

0

0

N

i

CytoplasmicMembrane

10

10

YTFR_ECOLI

Q6BEX0

ytfR

ytfS

b4485 JW5752

cytoplasmic peripheral IM protein, ABC transporter ATP-binding YtfR

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (By similarity)

Homolog to import ATP-binding protein MalK. Homolog to import ATP-binding protein MalK.

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

N/A

Cytoplasmic

pseudogene (Ochman & Davalos, 2006)

no

yes

N

25

0.128

CYT

0

-0.200913

-

0

0

N

i

CytoplasmicMembrane

10

24

ADPP_ECOLI

Q93K97

nudF

aspP yqiE yzzG

b3034 JW3002

cytoplasmic peripheral IM protein, ADP-ribose pyrophosphatase NudF

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)

[19766000]

Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm

A, F1

Soluble IM Fraction +

Cytoplasmic

yes

yes

N

38

0.101

CYT

0

-0.200913

-

0

0

N

i

Cytoplasmic

8

57