|
SepL
|
/
|
B7UM95
|
SctW
|
sepL
|
39
|
351
|
3 helical domains
|
Gatekeeper/Affinity switch
|
unknown
|
unknown
|
N/A
|
T3S
|
Translocators not secreted, Effectors oversecreted
|
-
|
Cytoplasm (weak interaction IM)
|
|
CesT EscV Secreted proteins and their chaperones SepD
|
Receptor at the membrane for translocation; Once absent the translocation switches to effectors
|
|
|
EscV
|
SepA
|
B7UMA7
|
SctV
|
escV
|
75
|
675
|
7 TM; large cytoplasmic domain
|
Major component, external to channel
|
9
|
676,8
|
N/A
|
Sec
|
-
|
-
|
Inner Membrane
|
|
EscJ Secreted proteins and their chaperones SepL
|
Receptor at the membrane for translocation; Translocation channel
|
|
|
EscU
|
/
|
B7UMB8
|
SctU
|
escU
|
39
|
345
|
3 TM; large cytoplasmic domain
|
Minor component, external to channel
|
1 to 2
|
39.2-78.4
|
cleaved at NPTH motif
|
Sec
|
-
|
-
|
Inner Membrane
|
|
EscI EscP EscR
|
Export apparatus; Switch mechanism from early to middle substrates
|
|
|
EscT
|
/
|
B7UMB9
|
SctT
|
escT
|
28
|
258
|
5 TM
|
Translocase channel
|
1
|
28,7
|
N/A
|
Sec
|
-
|
-
|
Inner Membrane
|
|
EscR EscS EscT
|
Export apparatus
|
|
|
EscS
|
/
|
B7UMC0
|
SctS
|
escS
|
9
|
89
|
2 TM
|
Translocase channel
|
1
|
9,9
|
N/A
|
Sec
|
-
|
-
|
Inner Membrane
|
|
EscR EscT
|
Export apparatus
|
|
|
EscR
|
/
|
B7UMC1
|
SctR
|
escR
|
24
|
217
|
4 TM
|
Translocase channel
|
5
|
121,5
|
N/A
|
Sec
|
-
|
-
|
Inner Membrane
|
|
EscI EscR EscS EscT EscU
|
Export apparatus
|
|
|
EscP
|
/
|
B7UMA4
|
SctP
|
orf16
|
16
|
138
|
C-terminal conserved T3S4 domain (type III secretion and substrate specificity switch) that interacts with SctU C-terminus
|
Molecular ruler
|
unknown
|
unknown
|
N/A
|
T3S
|
Less translocators
|
Not affected
|
Secretory protein
|
|
CesT EscF EscI EscU Orf16
|
Ruler for filament length; Switch mechanism from early to middle substrates
|
|
|
EscN
|
SepB
|
B7UMA6
|
SctN
|
escN
|
48
|
446
|
cytoplasmic; residues 103?371 contain a mixed ?/? Rossmann fold; highly helical
|
Hexameric ring-structure ATPase
|
6
|
293,1
|
N/A
|
No
|
-
|
-
|
Cytoplasm (weak interaction IM)
|
|
EscL EscO EscQ EscV Secreted proteins and their chaperones
|
ATPase (walker box motif). Forms hexameric complex
|
|
|
EscL
|
/
|
B7UMC2
|
SctL
|
orf5
|
27
|
231
|
helical protein
|
External stator connecting ATPase and Cytoplasmic ring
|
12
|
326,2
|
N/A
|
No
|
-
|
-
|
Cytoplasm (weak interaction IM)
|
N/A
|
EscN EscO EscQ EscV EspA
|
Negative regulator of ATPase
|
|
|
EscK
|
/
|
B7UMC3
|
SctK
|
orf4
|
23
|
199
|
N/A
|
Connector of Pods with SctD
|
6
|
140,8
|
N/A
|
No
|
-
|
-
|
Cytoplasm (weak interaction IM)
|
N/A
|
EscD EscQ
|
Connector of Pods with SctD
|
|
|
EscJ
|
/
|
B7UMB1
|
SctJ
|
escJ
|
20
|
190
|
1 large TM, periplasm-facing lipoprotein
|
Lipoprotein ring component
|
24
|
498,2
|
Palmitoylated at C19 (by analogy to PrgK PMID: 15931226)
|
Sec, Lipoprotein
|
-
|
-
|
Inner Membrane
|
|
EscD EscI EscJ EscV
|
IM ring
|
|
|
EscI
|
/
|
B7UMB0
|
SctI
|
rorf8
|
15
|
142
|
peripolasmic protein
|
Inner rod
|
3
|
45,9
|
N/A
|
T3S
|
-
|
-
|
Periplasm
|
|
EscC EscI EscP EscR EscT EscU EtgA
|
Inner rod protein assists in secretin insertion into OM
|
|
|
EscF
|
/
|
B7UM90
|
SctF
|
escF
|
8
|
73
|
helical protein
|
Needle component
|
120
|
974
|
N/A
|
T3S
|
-
|
-
|
Secretory protein
|
|
EscE EscF EscG EscP
|
Major Needle component
|
|
|
EspD
|
/
|
B7UM93
|
SctE
|
espD
|
39
|
380
|
2 TM, mainly hydrophylic
|
Translocon pore protein
|
8
|
316
|
N/A
|
T3S
|
Not affected
|
-
|
Host plasma membrane
|
|
CesD2 EspA EspB EspC EspD
|
Translocator, pore forming protein
|
|
|
EscD
|
/
|
B7UM96
|
SctD
|
escD
|
45
|
406
|
1 TM
|
Major IM ring component
|
24
|
1087,2
|
N/A
|
Sec
|
-
|
-
|
Inner Membrane
|
N/A
|
EscC EscJ EscK EscQ
|
EscD and EscJ form ring-like structure in inner membrane; BON domains mediate binding to phospholipids; FHA domain mediates protein-protein interactions and signalling.
|
|
|
EscC
|
/
|
B7UMB3
|
SctC
|
escC
|
56
|
512
|
N-terminal periplasmic domain; secretin family protein
|
Secretin
|
12 to 14
|
674,4
|
N/A
|
Sec
|
-
|
-
|
Outer Membrane
|
|
EscD EscI EscO
|
T3SS basal body and forms OM ring
|
|
|
EspB
|
eaeB
|
Q05129
|
SctB
|
espB
|
33
|
321
|
mainly hydrophylic and helical
|
Translocon pore protein
|
8
|
264,8
|
N/A
|
T3S
|
Not affected
|
-
|
Host plasma membrane
|
|
CesAB CesD EscN EscV EspA EspD SepL
|
Translocator, pore forming protein. Binds to myosins that interact with actin filaments and mediate essential processes (e.g.microvillus formation ,phagocytosis). Inhibits the interaction of myosins with actin PMID: 18078690.
|
|
|
EspA
|
/
|
B7UM94
|
SctA
|
espA
|
20
|
192
|
mainly helical
|
Filament protein
|
28 per turn
|
574
|
N/A
|
T3S
|
Not affected
|
-
|
Secretory protein
|
|
CesAB EscL EscN EscV EspB EspD SepL
|
Translocator. Half-flagellin model; dimerizes before polymerizes; lacks D0 and D3 domains.
|
|
|
N/A
|
/
|
B7UMC9
|
N/A
|
rorf1
|
31
|
272
|
N/A
|
N/A
|
unknown
|
unknown
|
N/A
|
N/A
|
unknown
|
+
|
N/A
|
N/A
|
|
SdiA-regulated domain (NHLrepeats). Unknown Function.
|
|
|
EspG
|
rorf2
|
B7UMC8
|
N/A
|
espG
|
43
|
398
|
cytoplasmic
|
Effector
|
more than injectisomes
|
unknown
|
N/A
|
T3S
|
Not affected
|
+
|
Host Cytoplasm
|
|
CesT
|
Translocated effector. Disassemble microtubules to induce RhoGTPase-mediated cytokeletal rearrangements that promote bacterial attachment, entry, or movement within the cytoplasm PMID: 15972534. Activating the host cell protease calpain.
|
|
|
Ler
|
orf1
|
B7UMC6
|
N/A
|
ler
|
15
|
129
|
cytoplasmic
|
Global Regulator
|
more than injectisomes
|
unknown
|
N/A
|
No
|
No expression
|
-
|
Cytoplasm
|
|
HNS Mcp/CesL StpA
|
Positive regulator of T3SS
|
|
|
EscE
|
/
|
B7UMC5
|
N/A
|
orf2
|
8
|
72
|
cytoplasmic
|
Chaperone of EscF
|
more than injectisomes
|
unknown
|
N/A
|
No
|
-
|
-
|
Cytoplasm
|
N/A
|
SepL
|
One of the chaperones of EscF
|
|
|
CesAB
|
/
|
B7UMC4
|
N/A
|
orf3
|
12
|
107
|
cytoplasmic
|
Chaperone (translocators)
|
more than injectisomes
|
unknown
|
N/A
|
No
|
No EspA, less EspB
|
-
|
Cytoplasm
|
N/A
|
EscN EscV EspA EspB SepL
|
Chaperone for EspA and EspB
|
|
|
EtgA
|
/
|
B7UMB7
|
N/A
|
rorf3
|
17
|
152
|
peripolasmic
|
Muramidase
|
unknown
|
unknown
|
N/A
|
Sec
|
-
|
+
|
Peptidoglycan
|
|
EscI
|
Muramidase that degrades peptidoglycan
|
|
|
GrlR
|
/
|
B7UMB6
|
N/A
|
orf10
|
13
|
123
|
cytoplasmic
|
Negative regulator
|
unknown
|
unknown
|
N/A
|
No
|
Not affected
|
+
|
Cytoplasm
|
N/A
|
GrlA
|
Negative regulator TTSS; avoiding GrlA binding to the LEE1 promoter and thus repressing ler expression
|
|
|
GrlA
|
/
|
B7UMB5
|
N/A
|
orf11
|
16
|
137
|
cytoplasmic
|
Positive regulator
|
unknown
|
unknown
|
N/A
|
No
|
No expression
|
-
|
Cytoplasm
|
N/A
|
GrlR
|
Positive regulator TTSS
|
|
|
CesD
|
/
|
B7UMB4
|
N/A
|
cesD
|
17
|
151
|
cytoplasmic
|
Subunit 2 of SctW chaperone heterodimer
|
1 per SctW
|
unknown
|
N/A
|
No
|
Translocators not secreted, Effectors oversecreted
|
-
|
Cytoplasm
|
|
EspB EspD
|
Chaperone for EspB and EspD
|
|
|
SepD
|
/
|
B7UMB2
|
N/A
|
rorf6
|
17
|
151
|
cytoplasmic
|
Subunit 1 of SctW chaperone heterodimer
|
1 per SctW
|
unknown
|
N/A
|
No
|
Translocators not secreted, Effectors oversecreted
|
-
|
Cytoplasm
|
N/A
|
CesL SepL
|
Chaperone of SepL together with CesL and regulator of switch mechanism
|
|
|
EspZ/SepZ
|
/
|
B7UMA9
|
N/A
|
espZ/sepZ
|
9
|
98
|
2TM
|
Effector
|
more than injectisomes
|
unknown
|
N/A
|
T3S
|
Not affected
|
+
|
Host Cytoplasm
|
N/A
|
EscR
|
Translocated effector; Contributes to host cell survival mechanisms
|
|
|
Mpc/CesL
|
/
|
B7UMA8
|
N/A
|
orf12
|
13
|
117
|
cytoplasmic
|
Chaperone (effectors)
|
1 per SctW
|
unknown
|
N/A
|
No
|
-
|
-
|
Cytoplasm
|
N/A
|
EscO Ler SepD SepL
|
Chaperone of SepL together with SepD and regulator of switch mechanism
|
|
|
EscO
|
/
|
B7UMA5
|
N/A
|
orf15
|
14
|
125
|
cytoplasmic
|
Central stalk, inserting in ATPase ring
|
1
|
14,7
|
N/A
|
No
|
-
|
-
|
Cytoplasm (weak interaction IM)
|
N/A
|
EscC EscG EscL EscN EscV Mcp/CesL
|
central stalk attached to C-terminus of EscV; stabilizes the EscN hexamer and stimulates its ATPase activity
|
|
|
EscQ
|
SepQ
|
B7UMA3
|
N/A
|
escQ
|
34
|
305
|
cytoplasmic
|
Component of 6 Pod assembly
|
12
|
419,6
|
N/A
|
No
|
-
|
-
|
Cytoplasm (weak interaction IM)
|
N/A
|
EscD EscK EscL EscN
|
Forms ring within basal body
|
|
|
EspH
|
orf18
|
B7UMA2
|
N/A
|
espH
|
19
|
168
|
secreted
|
Effector
|
more than injectisomes
|
unknown
|
N/A
|
T3S
|
Not affected
|
+
|
Host Cytoplasm
|
N/A
|
CesT
|
Translocated effector
|
|
|
CesF
|
rorf10
|
B7UMA1
|
N/A
|
cesF
|
14
|
120
|
cytoplasmic
|
Chaperone (effectors)
|
more than injectisomes
|
unknown
|
N/A
|
No
|
Not affected
|
+
|
Cytoplasm
|
|
EspF
|
Chaperone for EspF
|
|
|
Map
|
/
|
B7UMA0
|
N/A
|
orf19
|
22
|
203
|
secreted
|
Effector
|
more than injectisomes
|
unknown
|
N/A
|
T3S
|
Not affected
|
+
|
Host Cytoplasm
|
|
CesT
|
Mitochondrial dysfunction; Induces transient filopodia formation
|
|
|
Tir
|
Hp90
|
B7UM99
|
N/A
|
tir
|
56
|
550
|
secreted
|
Effector
|
more than injectisomes
|
unknown
|
pY474 by host kinases; pY454 by host kinases PMID: 15220932; pS436, pS437 by host kinases PMID: 16954405.
|
T3S
|
Not affected
|
-
|
Host cytoplasmic membrane
|
|
CesT EscN
|
N-terminal and C-terminal domains remain intracellular and interact with numerous signalling, adapter and cytoskeletal proteins. Function dependent on its phosphorylation sites, a GTPase activating protein (GAP)-like motif and a polyproline region. Phosphorylation affects oligomerization.
|
|
|
CesT
|
orfU
|
P21244
|
N/A
|
cesT
|
17
|
156
|
cytoplasmic
|
Chaperone (effectors)
|
more than injectisomes
|
unknown
|
N/A
|
No
|
Not affected
|
-
|
Cytoplasm
|
|
CesT EscN EspF EspG EspH EspZ GapA Map NleA NleF NleG NleH NleH2 SepL Tir
|
Chaperone of Tir and Map
|
|
|
Intimin
|
eaeA
|
P19809
|
N/A
|
eae
|
102
|
939
|
secreted
|
Attaching and effacing protein
|
more than injectisomes
|
unknown
|
N/A
|
T3S
|
Not affected
|
-
|
Bacterial outer membrane
|
N/A
|
Tir
|
Adhesin on the bacterial membrane
|
|
|
CesD2
|
/
|
B7UM91
|
N/A
|
orf27
|
15
|
135
|
cytoplasmic
|
Chaperone (translocators)
|
more than injectisomes
|
unknown
|
N/A
|
No
|
Less EspD
|
Not affected
|
Cytoplasm
|
|
EspB EspD
|
Chaperone of EspD. Homologous LcrR will bind YopB.
|
|
|
EscG
|
/
|
B7UM89
|
N/A
|
orf29
|
10
|
92
|
cytoplasmic
|
Chaperone of EscF
|
more than injectisomes
|
unknown
|
N/A
|
No
|
-
|
-
|
Cytoplasm
|
N/A
|
EscE EscF EscO
|
One of the chaperones of EscF
|
|
|
EspF
|
orfd4
|
B7UM88
|
N/A
|
espF
|
20
|
206
|
secreted
|
Effector
|
more than injectisomes
|
unknown
|
N/A
|
T3S
|
Not affected
|
+
|
Host Cytoplasm
|
|
CesF CesT
|
Translocated effector. Mitochondrial dysfunction. Microvilli effacement. TJ disruption, apoptosis. Epithelial transporter inhibition. Antiphagocytosis. Membrane remodeling. Actin-pedestal maturation. Nucleolus.
|
|
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