E. coli K-12 / MG1655/ ATCC 47076 proteome

UniProt Name:
Curli production assembly/transport component CsgG
Localization:
Outer Membrane Lipoprotein, Peripheral outer membrane protein facing the extra-cellular space (Experimental) Outer Membrane Lipoprotein, Peripheral outer membrane protein facing the extra-cellular space (Probable)
Notes:
Curli component. Proven signal peptidase II substrate. CsgG is an outer membrane protein required for the secretion of the two curli structural subunits CsgA and CsgB. CsgG is a lipoprotein that spans the outermembrane and assembles into an oligomeric annular-shaped structure with an inner diameter of approximately 2 nm (Margery L. Evans and Matthew R. Chapman in press). Overexpression of CsgG results in increased sensitivity to the small antibiotic erythromycin (740Da) but not the larger vancomycin (1440Da) suggesting that CsgG has a pore-like activity (Robinson et al 2006). PMID: 15174130
References:
PMID: 15174130 PMID: 21645131
Pseudogene/Mobile Element:
-

mRNA molec/cell:
1

Proteomics:
PMID: 17595003
Proteomics / Inner Membrane fraction:
-

Protein molec/cell:
2
PaxDb:
22.4 ppm

Class:
-
Fold:
-
Super Family:
-

Go to Complexes

UniProt Name:
Flagellar L-ring protein (Basal body L-ring protein)
Localization:
Outer Membrane Lipoprotein (Experimental)
Notes:
Proven pignal peptidase II substrate. L ring protein. PMID: 15174130
References:
PMID: 15174130 PMID: 20932056
Pseudogene/Mobile Element:
-

mRNA molec/cell:
-

Proteomics:
PMID: 19767571 PMID: 20932056
Proteomics / Inner Membrane fraction:
-

Protein molec/cell:
-
PaxDb:
75.5 ppm

Class:
-
Fold:
-
Super Family:
-

Go to Complexes

UniProt Name:
Putative polysaccharide export protein Wza
Localization:
Outer Membrane Lipoprotein, Peripheral outer membrane protein facing the extra-cellular space (Experimental) Outer Membrane Lipoprotein, Peripheral outer membrane protein facing the extra-cellular space (Probable)
Notes:
Has a helical structure that crosses membrane. The transmembrane region of Wza is an alpha-helical barrel. Proven pignal peptidase II substrate. It has been demostrated that it can be also secreted and be surface exposed. PMID: 15174130
References:
PMID: 10619844 PMID: 15174130 PMCID: PMC3315050
Pseudogene/Mobile Element:
-

mRNA molec/cell:
-

Proteomics:
PMID: 16606699 PMID: 20222674 PMID: 15115803
Proteomics / Inner Membrane fraction:
-

Protein molec/cell:
-
PaxDb:
0.366 ppm

Class:
-
Fold:
-
Super Family:
-

Go to Complexes

UniProt Name:
Major outer membrane prolipoprotein Lpp [Cleaved into: Major outer membrane lipoprotein Lpp (Braun lipoprotein) (Murein-lipoprotein)]
Localization:
Outer Membrane Lipoprotein, Peripheral outer membrane protein facing the extra-cellular space (Experimental) Outer Membrane Lipoprotein, Peripheral outer membrane protein facing the extra-cellular space (Probable)
Notes:
Trigger factor-sensitive aggregation. Proven pignal peptidase II substrate. Outer membrane protein determined by 2D gel electrophoresis (Lopez-Campistrous et al 2005). Covalently attached to the peptidoglycan cell wall by the ??-amino group of its C-terminal Lys residue; stabilizes the bacterial envelope. Identified in nucleoids collected by sucrose gradient centrifugation and analyzed by liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS). PMID: 21541338 PMID: 4575979
References:
PMID: 15174130 PMID: 15911532 PMID: 16079137 PMID: 3013869 PMID: 4575979
Pseudogene/Mobile Element:
-

mRNA molec/cell:
-

Proteomics:
PMID: 12488465 PMID: 19348461 PMID: 20222674 PMID: 16967446 PMID: 16858726 PMID: 19767571 PMID: 16079137 PMID: 15115803 PMID: 20932056 PMID: 19343722 PMID: 20923772
Proteomics / Inner Membrane fraction:
PMID: 16079137 PMID: 17309111

Protein molec/cell:
-
PaxDb:
6110 ppm

Class:
Coiled coil proteins
SCOP: 57942
Fold:
Parallel coiled-coil SCOP: 57943
Super Family:
Outer membrane lipoprotein
SCOP: 58042

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UniProt Name:
Cation efflux system protein CusC
Localization:
Outer Membrane Lipoprotein (Experimental) Outer Membrane Lipoprotein (Probable)
Notes:
Proven pignal peptidase II substrate. PMID: 15174130
References:
PMID: 15174130 PMID: 20932056 PMID: 21249122
Pseudogene/Mobile Element:
-

mRNA molec/cell:
-

Proteomics:
PMID: 16967446 PMID: 20932056
Proteomics / Inner Membrane fraction:
-

Protein molec/cell:
-
PaxDb:
65.2 ppm

Class:
Membrane and cell surface proteins and peptides
SCOP: 56835
Fold:
Outer membrane efflux proteins (OEP) SCOP: 56953
Super Family:
Outer membrane efflux proteins (OEP)
SCOP: 56954

Go to Complexes

UniProt Name:
Endo-type membrane-bound lytic murein transglycosylase A (EC 4.2.2.n2) (Peptidoglycan lytic endotransglycosylase)
Localization:
Outer Membrane Lipoprotein (Experimental)
Notes:
Proven pignal peptidase II substrate. PMID: 15174130
References:
PMID: 15174130
Pseudogene/Mobile Element:
-

mRNA molec/cell:
-

Proteomics:
PMID: 16606699 PMID: 19348461 PMID: 20222674 PMID: 19767571 PMID: 15115803
Proteomics / Inner Membrane fraction:
-

Protein molec/cell:
-
PaxDb:
0 ppm

Class:
Alpha and beta proteins (a+b)
SCOP: 53931
Fold:
Lysozyme-like
SCOP: 53954
Super Family:
Lysozyme-like
SCOP: 53955

Go to Complexes

UniProt Name:
Endolytic peptidoglycan transglycosylase RlpA (EC 4.2.2.-) (Rare lipoprotein A)
Localization:
Outer Membrane Lipoprotein (Experimental)
Notes:
Proven pignal peptidase II substrate. Outer membrane protein determined by LC-MS (Gevaert et al 2002). Outer membrane protein determined by 2D gel electrophoresis (Lopez-Campistrous et al 2005). Contains a SPOR domain. PMID: 15174130
References:
PMID: 15174130 PMID: 15911532 PMID: 19880599 PMID: 20932056
Pseudogene/Mobile Element:
-

mRNA molec/cell:
-

Proteomics:
PMID: 19348461 PMID: 20222674 PMID: 19767571 PMID: 15115803 PMID: 20932056 PMID: 19343722
Proteomics / Inner Membrane fraction:
-

Protein molec/cell:
-
PaxDb:
33.8 ppm

Class:
Alpha and beta proteins (a+b)
SCOP: 53931
Fold:
Double psi beta-barrel
SCOP: 50684
Ferredoxin-like
SCOP: 54861
Super Family:
Barwin-like endoglucanases
SCOP: 50685
Sporulation related repeat
SCOP: 110997

Go to Complexes

UniProt Name:
Entericidin A
Localization:
Outer Membrane Lipoprotein (Experimental)
Notes:
Small membrane protein detected experimentally. Proven pignal peptidase II substrate. PMID: 15174130
References:
PMID: 15174130
Pseudogene/Mobile Element:
-

mRNA molec/cell:
-

Proteomics:
PMID: 20222674 PMID: 19767571
Proteomics / Inner Membrane fraction:
-

Protein molec/cell:
-
PaxDb:
0.034 ppm

Class:
-
Fold:
-
Super Family:
-

Go to Complexes

UniProt Name:
Entericidin B
Localization:
Outer Membrane Lipoprotein (Experimental)
Notes:
Potentially toxic protein. Proven pignal peptidase II substrate. Identified in nucleoids collected by sucrose gradient centrifugation and analyzed by liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS). PMID: 21541338 PMID: 15174130
References:
PMID: 15174130 PMID: 20932056
Pseudogene/Mobile Element:
-

mRNA molec/cell:
-

Proteomics:
PMID: 19348461 PMID: 20222674 PMID: 19767571 PMID: 20932056 PMID: 19343722
Proteomics / Inner Membrane fraction:
PMID: 17309111

Protein molec/cell:
-
PaxDb:
634 ppm

Class:
-
Fold:
-
Super Family:
-

Go to Complexes

UniProt Name:
Formate-dependent nitrite reductase complex subunit NrfG
Localization:
-
Notes:
-
References:
-
Pseudogene/Mobile Element:
-

mRNA molec/cell:
-

Proteomics:
-
Proteomics / Inner Membrane fraction:
-

Protein molec/cell:
-
PaxDb:
0 ppm

Class:
All alpha proteins
SCOP: 46456
Fold:
alpha-alpha superhelix SCOP: 48370
Super Family:
TPR-like
SCOP: 48452

Go to Complexes

UniProt Name:
Intermembrane phospholipid transport system lipoprotein MlaA
Localization:
Outer Membrane Lipoprotein (Experimental)
Notes:
Proven pignal peptidase II substrate. PMID: 15174130
References:
PMID: 15174130 PMID: 20932056
Pseudogene/Mobile Element:
-

mRNA molec/cell:
-

Proteomics:
PMID: 17595003 PMID: 17137328 PMID: 19348461 PMID: 20222674 PMID: 16967446 PMID: 19767571 PMID: 15115803 PMID: 20932056 PMID: 19343722 PMID: 17446557
Proteomics / Inner Membrane fraction:
-

Protein molec/cell:
-
PaxDb:
22.4 ppm

Class:
-
Fold:
-
Super Family:
-

Go to Complexes

UniProt Name:
LPS-assembly lipoprotein LptE (Rare lipoprotein B)
Localization:
Outer Membrane Lipoprotein (Experimental)
Notes:
Proven signal peptidase II substrate. PMID: 16861298
References:
PMID: 15174130 PMID: 16861298 PMID: 20932056
Pseudogene/Mobile Element:
-

mRNA molec/cell:
-

Proteomics:
PMID: 16606699 PMID: 19348461 PMID: 20222674 PMID: 16967446 PMID: 19767571 PMID: 20803007 PMID: 15115803 PMID: 20932056 PMID: 19343722
Proteomics / Inner Membrane fraction:
-

Protein molec/cell:
-
PaxDb:
109 ppm

Class:
-
Fold:
-
Super Family:
-

Go to Complexes

UniProt Name:
Membrane-bound lysozyme inhibitor of C-type lysozyme
Localization:
Outer Membrane Lipoprotein (Probable)
Notes:
Disulfide bridge (37 102) PMID: 16937244
References:
PMID: 18369469
Pseudogene/Mobile Element:
-

mRNA molec/cell:
-

Proteomics:
PMID: 19348461 PMID: 20222674 PMID: 19767571
Proteomics / Inner Membrane fraction:
-

Protein molec/cell:
-
PaxDb:
0 ppm

Class:
All beta proteins
SCOP: 48724
Fold:
Streptavidin-like SCOP: 50875
Super Family:
YdhA-like
SCOP: 141488

Go to Complexes

UniProt Name:
Membrane-bound lytic murein transglycosylase A (EC 4.2.2.n1) (Mlt38) (Murein hydrolase A)
Localization:
Outer Membrane Lipoprotein (Experimental)
Notes:
The formation of a complex between a murein polymerase (PBP1B) and a murein hydrolase (MltA). In the presence of MipA represents a first step in a reconstitution of the hypothetical murein-synthesizing holoenzyme. Postulated to be responsible for controlled growth of the stress-bearing sacculus of <i>E.coli</i>. Proven pignal peptidase II substrate. Outer membrane protein determined by 2D gel electrophoresis (Lopez-Campistrous et al 2005, Fountoulakis et al 2003) PMID: 15174130 SRP recognizes the first signal anchor sequence of a polytopic membrane protein (MtlA) resulting in cotranslational targeting of MtlA to SecY and phospholipids of the plasma membrane. PMID: 10619852 Possible interaction of SRP with the secretory protein pOmpA is prevented by the association of trigger factor with nascent pOmpA. PMID: 10619852
References:
PMID: 10619852 PMID: 15174130 PMID: 20932056
Pseudogene/Mobile Element:
pseudogene (Ochman & Davalos, 2006)

mRNA molec/cell:
4

Proteomics:
PMID: 12624733 PMID: 19348461 PMID: 20222674 PMID: 16967446 PMID: 19767571 PMID: 15115803 PMID: 20932056 PMID: 19343722
Proteomics / Inner Membrane fraction:
-

Protein molec/cell:
1
PaxDb:
8.05 ppm

Class:
All beta proteins
SCOP: 48724
Fold:
Double psi beta-barrel SCOP: 50684
Super Family:
Barwin-like endoglucanases
SCOP: 50685

Go to Complexes

UniProt Name:
Membrane-bound lytic murein transglycosylase B (EC 4.2.2.n1) (35 kDa soluble lytic transglycosylase) (Murein hydrolase B) (Slt35)
Localization:
Outer Membrane Lipoprotein (Experimental)
Notes:
Proven pignal peptidase II substrate. PMID: 15174130
References:
PMID: 15174130 PMID: 20932056
Pseudogene/Mobile Element:
-

mRNA molec/cell:
0

Proteomics:
PMID: 19348461 PMID: 20222674 PMID: 19767571 PMID: 20932056
Proteomics / Inner Membrane fraction:
PMID: 17309111

Protein molec/cell:
0
PaxDb:
8.76 ppm

Class:
Alpha and beta proteins (a+b)
SCOP: 53931
Fold:
Lysozyme-like SCOP: 53954
Super Family:
Lysozyme-like
SCOP: 53955

Go to Complexes

UniProt Name:
Membrane-bound lytic murein transglycosylase C (EC 4.2.2.n1) (Murein lyase C)
Localization:
Outer Membrane Lipoprotein (Experimental)
Notes:
Proven pignal peptidase II substrate. PMID: 15174130
References:
PMID: 15174130 PMID: 20932056
Pseudogene/Mobile Element:
-

mRNA molec/cell:
-

Proteomics:
PMID: 19348461 PMID: 20222674 PMID: 16967446 PMID: 19767571 PMID: 20932056 PMID: 19343722
Proteomics / Inner Membrane fraction:
-

Protein molec/cell:
-
PaxDb:
5.77 ppm

Class:
Alpha and beta proteins (a+b)
SCOP: 53931
Fold:
Lysozyme-like
SCOP: 53954
Super Family:
Lysozyme-like
SCOP: 53955

Go to Complexes

UniProt Name:
Membrane-bound lytic murein transglycosylase D (EC 4.2.2.n1) (Murein hydrolase D) (Regulatory protein DniR)
Localization:
Outer Membrane Lipoprotein (Experimental)
Notes:
Proven signal peptidase II substrate. PMID: 15174133
References:
PMID: 15174130 PMID: 15174133
Pseudogene/Mobile Element:
-

mRNA molec/cell:
-

Proteomics:
PMID: 20222674
Proteomics / Inner Membrane fraction:
-

Protein molec/cell:
0
PaxDb:
1.24 ppm

Class:
Alpha and beta proteins (a+b)
SCOP: 53931
Fold:
Lysozyme-like
SCOP: 53954
LysM domain
SCOP: 54105
Super Family:
Lysozyme-like
SCOP: 53955
LysM domain
SCOP: 54106

Go to Complexes

UniProt Name:
Metalloprotease LoiP (EC 3.4.24.-)
Localization:
Outer Membrane Lipoprotein (Experimental) Outer Membrane Lipoprotein (Probable)
Notes:
Disulfide bridge (53 108) PMID: 22491786
References:
PMID: 17909889 PMID: 20932056 PMID: 22491786
Pseudogene/Mobile Element:
-

mRNA molec/cell:
-

Proteomics:
PMID: 16606699 PMID: 19348461 PMID: 20222674 PMID: 19767571 PMID: 15115803 PMID: 20932056
Proteomics / Inner Membrane fraction:
-

Protein molec/cell:
-
PaxDb:
41.2 ppm

Class:
-
Fold:
-
Super Family:
-

Go to Complexes

UniProt Name:
Metalloprotease YcaL (EC 3.4.-.-)
Localization:
Outer Membrane Lipoprotein (Experimental)
Notes:
Proven pignal peptidase II substrate. PMID: 15174130
References:
PMID: 15174130
Pseudogene/Mobile Element:
-

mRNA molec/cell:
-

Proteomics:
PMID: 19343722
Proteomics / Inner Membrane fraction:
-

Protein molec/cell:
-
PaxDb:
0 ppm

Class:
-
Fold:
-
Super Family:
-

Go to Complexes

UniProt Name:
Multidrug resistance protein MdtE
Localization:
Outer Membrane Lipoprotein (Experimental)
Notes:
Outer membrane protein related to resistance to ampicillin and tetracycline Proven pignal peptidase II substrate.
References:
PMID: 15174130
Pseudogene/Mobile Element:
-

mRNA molec/cell:
-

Proteomics:
PMID: 16606699 PMID: 17137328 PMID: 19348461 PMID: 20222674 PMID: 19767571 PMID: 16079137 PMID: 15115803 PMID: 18796603
Proteomics / Inner Membrane fraction:
PMID: 16079137 PMID: 17446557 PMID: 17309111

Protein molec/cell:
0
PaxDb:
32.2 ppm

Class:
Membrane and cell surface proteins and peptides
SCOP: 56835
Fold:
HlyD-like secretion proteins SCOP: 111368
Super Family:
HlyD-like secretion proteins
SCOP: 111369

Go to Complexes