E. coli K-12 / MG1655/ ATCC 47076 proteome

UniProt Name:
Flagella synthesis protein FlgN
Localization:
-
Notes:
Substrate-specific chaperone of the flagellar export system along with FliT and FliS. Protects FlgK, FlgL from proteolysis. Interaction with FlhA which is an integral inner membrane protein isInvolved in efficient protein export.
References:
-
Pseudogene/Mobile Element:
-

mRNA molec/cell:
-

Proteomics:
PMID: 20923772
Proteomics / Inner Membrane fraction:
-

Protein molec/cell:
-
PaxDb:
109 ppm

Class:
All alpha proteins
SCOP: 46456
Fold:
STAT-like SCOP: 47654
Super Family:
FlgN-like
SCOP: 140566

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UniProt Name:
Peptidoglycan hydrolase FlgJ (EC 3.2.1.-) (Muramidase FlgJ)
Localization:
-
Notes:
Rod capping protein/ muramidase
References:
-
Pseudogene/Mobile Element:
-

mRNA molec/cell:
-

Proteomics:
PMID: 16606699
Proteomics / Inner Membrane fraction:
-

Protein molec/cell:
-
PaxDb:
0.021 ppm

Class:
-
Fold:
-
Super Family:
-

Go to Complexes

UniProt Name:
Negative regulator of flagellin synthesis (Anti-sigma-28 factor)
Localization:
Cytoplasmic, Extra-cellular (Probable)
Notes:
Anti-sigma28 factor. FlgM is an anti-sigma factor that binds and inactivates sigma28 (FliA_Ecoli). Once the hook and basal body is assembled, FlgM is secreted by the flagellar export system thereby freeing sigma28 to activate transcription.
References:
PMID: 9988479
Pseudogene/Mobile Element:
-

mRNA molec/cell:
-

Proteomics:
PMID: 16606699 PMID: 20222674 PMID: 20803007
Proteomics / Inner Membrane fraction:
-

Protein molec/cell:
-
PaxDb:
251 ppm

Class:
All alpha proteins
SCOP: 46456
Fold:
Non-globular all-alpha subunits of globular proteins
SCOP: 48661
Super Family:
Anti-sigma factor FlgM
SCOP: 101498

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UniProt Name:
Basal-body rod modification protein FlgD
Localization:
Peripheral outer membrane protein facing the extra-cellular space (Experimental)
Notes:
Hook-capping protein. The hook cap FlgD is displaced by FlgK, allowing filament growth PMID: 12631703 Using the hook scaffolding protein FlgD of Escherichia coli as a model substrate, it is demonstrated that the export signal is contained within the N-terminal 71 amino acids of FlgD. PMID: 16897036 PMID: 16897036
References:
PMID: 12631703
Pseudogene/Mobile Element:
pseudogene (Ochman & Davalos, 2006)

mRNA molec/cell:
-

Proteomics:
-
Proteomics / Inner Membrane fraction:
-

Protein molec/cell:
-
PaxDb:
0 ppm

Class:
-
Fold:
-
Super Family:
-

Go to Complexes

UniProt Name:
Flagellin
Localization:
Peripheral outer membrane protein facing the extra-cellular space (Experimental) Peripheral outer membrane protein facing the extra-cellular space (Probable)
Notes:
Flameint protein, flagellin. Flagella proteins contribute to the production of outer membrane vesicles. Analysis of E. coli W3110 OMVs revealed that they contain abundant levels of FliC. Compared to the parental strain expressing wild-type fliC, an E. coli strain with a fliC-null mutation produced reduced amounts of OMVs based on both protein and phosphate levels. Identified in nucleoids collected by sucrose gradient centrifugation and analyzed by liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS). PMID: 21541338 PMID: 12730325
References:
PMID: 12730325 PMID: 24134841
Pseudogene/Mobile Element:
-

mRNA molec/cell:
-

Proteomics:
PMID: 16606699 PMID: 17595003 PMID: 17137328 PMID: 20222674 PMID: 19767571
Proteomics / Inner Membrane fraction:
PMID: 17309111

Protein molec/cell:
-
PaxDb:
1212 ppm

Class:
Multi-domain proteins (alpha and beta)
SCOP: 56572
Fold:
Phase 1 flagellin SCOP: 64517
Super Family:
Phase 1 flagellin
SCOP: 64518

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UniProt Name:
Flagellar hook protein FlgE
Localization:
Peripheral outer membrane protein facing the extra-cellular space (Experimental)
Notes:
Hook protein. PMID: 12730325
References:
PMID: 12730325
Pseudogene/Mobile Element:
pseudogene (Ochman & Davalos, 2006)

mRNA molec/cell:
-

Proteomics:
PMID: 19767571 PMID: 18796603
Proteomics / Inner Membrane fraction:
-

Protein molec/cell:
-
PaxDb:
17.2 ppm

Class:
All beta proteins
SCOP: 48724
Fold:
Flagellar hook protein flgE SCOP: 117142
Super Family:
Flagellar hook protein flgE
SCOP: 117143

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UniProt Name:
Flagellar hook-associated protein 1 (HAP1)
Localization:
Peripheral outer membrane protein facing the extra-cellular space (Experimental) Peripheral outer membrane protein facing the extra-cellular space (Probable)
Notes:
Part of FlgN-FlgK-FliJ-FlhA complex. First hook-filament junction protein. Flagella proteins contribute to the production of outer membrane vesicles. Analysis of E. coli W3110 OMVs revealed that they contain abundant levels of FliC. Compared to the parental strain expressing wild-type flgK, an E. coli strain with a flgK-null mutation produced reduced amounts of OMVs. PMID: 12730325
References:
PMID: 12730325 PMID: 24134841
Pseudogene/Mobile Element:
-

mRNA molec/cell:
-

Proteomics:
PMID: 16606699 PMID: 19767571 PMID: 17446557
Proteomics / Inner Membrane fraction:
-

Protein molec/cell:
-
PaxDb:
56.2 ppm

Class:
Multi-domain proteins (alpha and beta)
SCOP: 56572
Fold:
Phase 1 flagellin SCOP: 64517
Super Family:
Phase 1 flagellin
SCOP: 64518

Go to Complexes

UniProt Name:
Flagellar hook-associated protein 3 (HAP3) (Hook-filament junction protein)
Localization:
Peripheral outer membrane protein facing the extra-cellular space (Experimental)
Notes:
Second hook-filament junction protein. PMID: 12730325
References:
PMID: 12730325
Pseudogene/Mobile Element:
-

mRNA molec/cell:
-

Proteomics:
PMID: 16606699 PMID: 19767571 PMID: 17446557
Proteomics / Inner Membrane fraction:
-

Protein molec/cell:
-
PaxDb:
67.6 ppm

Class:
Multi-domain proteins (alpha and beta)
SCOP: 56572
Fold:
Phase 1 flagellin SCOP: 64517
Super Family:
Phase 1 flagellin
SCOP: 64518

Go to Complexes

UniProt Name:
Flagellar L-ring protein (Basal body L-ring protein)
Localization:
Outer Membrane Lipoprotein (Experimental)
Notes:
Proven pignal peptidase II substrate. L ring protein. PMID: 15174130
References:
PMID: 15174130 PMID: 20932056
Pseudogene/Mobile Element:
-

mRNA molec/cell:
-

Proteomics:
PMID: 19767571 PMID: 20932056
Proteomics / Inner Membrane fraction:
-

Protein molec/cell:
-
PaxDb:
75.5 ppm

Class:
-
Fold:
-
Super Family:
-

Go to Complexes

UniProt Name:
Flagellar basal body rod protein FlgB (Putative proximal rod protein)
Localization:
Periplasmic (By similarity) Periplasmic (Experimental)
Notes:
Proximal rod protein. PMID: 12730325 FlgG is a distal component of the basal body rod. While FlgF was highly soluble, FlgB, FlgC, FlgG and FliE tended to self or cross-aggregate into fibrils in solutions at neutral pH or below, at high ionic strength, or at high protein concentration. PMID: 15136044
References:
PMID: 12631703 PMID: 12730325 PMID: 15136044
Pseudogene/Mobile Element:
-

mRNA molec/cell:
-

Proteomics:
PMID: 12488465 PMID: 20222674 PMID: 17446557
Proteomics / Inner Membrane fraction:
-

Protein molec/cell:
-
PaxDb:
0.696 ppm

Class:
-
Fold:
-
Super Family:
-

Go to Complexes

UniProt Name:
Flagellar basal-body rod protein FlgC (Putative proximal rod protein)
Localization:
Periplasmic (By similarity) Periplasmic (Experimental)
Notes:
Proximal rod protein. PMID: 12730325 FlgB, FlgC, FlgF, and FlgG are four proteins that comprise the rod section of the basal-body assembly of the flagellar motor. The rod is a rotational component which is situated transverse to the outer membrane and peptidoglycan layers and is circumscribed by a set of four rings which lie in a horizontal plane within the inner and outer membranes of the cell envelope. PMID: 2181149 FlgG is a distal component of the basal body rod. While FlgF was highly soluble, FlgB, FlgC, FlgG and FliE tended to self or cross-aggregate into fibrils in solutions at neutral pH or below, at high ionic strength, or at high protein concentration. PMID: 15136044
References:
PMID: 12631703 PMID: 12730325 PMID: 15136044 PMID: 2181149
Pseudogene/Mobile Element:
-

mRNA molec/cell:
-

Proteomics:
PMID: 16606699 PMID: 19767571
Proteomics / Inner Membrane fraction:
-

Protein molec/cell:
-
PaxDb:
0 ppm

Class:
-
Fold:
-
Super Family:
-

Go to Complexes

UniProt Name:
Flagellar basal-body rod protein FlgF (Putative proximal rod protein)
Localization:
Periplasmic (By similarity) Periplasmic (Experimental)
Notes:
Homolog to flgG. Proximal rod protein. PMID: 12730325 FlgB, FlgC, FlgF, and FlgG are four proteins that comprise the rod section of the basal-body assembly of the flagellar motor. The rod is a rotational component which is situated transverse to the outer membrane and peptidoglycan layers and is circumscribed by a set of four rings which lie in a horizontal plane within the inner and outer membranes of the cell envelope. PMID: 2181149 FlgG is a distal component of the basal body rod. While FlgF was highly soluble, FlgB, FlgC, FlgG and FliE tended to self or cross-aggregate into fibrils in solutions at neutral pH or below, at high ionic strength, or at high protein concentration. PMID: 15136044
References:
PMID: 12730325 PMID: 15136044 PMID: 2181149
Pseudogene/Mobile Element:
-

mRNA molec/cell:
-

Proteomics:
-
Proteomics / Inner Membrane fraction:
-

Protein molec/cell:
-
PaxDb:
0 ppm

Class:
All beta proteins
SCOP: 48724
Fold:
Flagellar hook protein flgE SCOP: 117142
Super Family:
Flagellar hook protein flgE
SCOP: 117143

Go to Complexes

UniProt Name:
Flagellar basal-body rod protein FlgG (Distal rod protein)
Localization:
Periplasmic (By similarity) Periplasmic (Experimental)
Notes:
Distal rod protein PMID: 12730325 FlgB, FlgC, FlgF, and FlgG are four proteins that comprise the rod section of the basal-body assembly of the flagellar motor. The rod is a rotational component which is situated transverse to the outer membrane and peptidoglycan layers and is circumscribed by a set of four rings which lie in a horizontal plane within the inner and outer membranes of the cell envelope. PMID: 2181149 FlgG is a distal component of the basal body rod. While FlgF was highly soluble, FlgB, FlgC, FlgG and FliE tended to self or cross-aggregate into fibrils in solutions at neutral pH or below, at high ionic strength, or at high protein concentration. PMID: 15136044
References:
PMID: 12631703 PMID: 12730325 PMID: 15136044 PMID: 2181149
Pseudogene/Mobile Element:
-

mRNA molec/cell:
-

Proteomics:
PMID: 19767571
Proteomics / Inner Membrane fraction:
-

Protein molec/cell:
-
PaxDb:
5.58 ppm

Class:
All beta proteins
SCOP: 48724
Fold:
Flagellar hook protein flgE
SCOP: 117142
Super Family:
Flagellar hook protein flgE
SCOP: 117143

Go to Complexes

UniProt Name:
Flagellar P-ring protein (Basal body P-ring protein)
Localization:
Periplasmic (Experimental)
Notes:
P ring protein. Substrate of periplasmic disulfide oxidoreductases DsbA. Might be is co-translationally translocated through Sec translocon. Identified DsbA substrate PMID: 19953543 PMID: 12730325 Potential substrate for the SRP pathway. When fused to cytoplasmic protein thioredoxin promotes signal recognition particle-dependent translocation PMID: 15838024
References:
PMID: 12730325 PMID: 15838024
Pseudogene/Mobile Element:
-

mRNA molec/cell:
-

Proteomics:
PMID: 20222674
Proteomics / Inner Membrane fraction:
-

Protein molec/cell:
-
PaxDb:
0.45 ppm

Class:
-
Fold:
-
Super Family:
-

Go to Complexes

UniProt Name:
Flagella basal body P-ring formation protein FlgA
Localization:
Periplasmic (Experimental)
Notes:
Periplasmic chaperone for P ring protein. PMID: 12730325
References:
PMID: 12730325
Pseudogene/Mobile Element:
-

mRNA molec/cell:
-

Proteomics:
PMID: 16606699 PMID: 17446557
Proteomics / Inner Membrane fraction:
-

Protein molec/cell:
-
PaxDb:
0.477 ppm

Class:
-
Fold:
-
Super Family:
-

Go to Complexes