STEPdb: Sub-cellular Topologies of E.coli Polypeptides.

View: E. coli K-12 / MG1655/ ATCC 47076 proteome Export to Excel Export to HTML Export to XML Export to CSV

E. coli K-12 / MG1655/ ATCC 47076 proteome

(mouse over and click on the one letter topology symbols to see which proteins of this specific subproteome are localized in each location)

Bacterial cell cartoon of sub-cellular locations

show distribution

Manual Curation in STEP

Comments

UniProt Name:
Outer membrane protein A (Outer membrane protein II*)
Localization:
Outer Membrane b-barrel protein (Experimental) Outer Membrane b-barrel protein (Potential) Outer Membrane b-barrel protein (Probable)
Notes:
Substrate of periplasmic peptidyl prolyl cis???trans isomerase SurA. Potential pubstrate of periplasmic chaperones Skp. Trigger factor-sensitive aggregation. Trigger factor-sensitive aggregation. Copurifies with trigger factor in pull down assays. The Lipid Bilayer-Inserted Membrane Protein BamA of Escherichia coli Facilitates Insertion and Folding of Outer Membrane Protein A from Its Complex with Skp. Outer membrane protein determined by LC-MS (Gevaert et al 2002). Outer membrane protein determined by 2D gel electrophoresis (Lopez-Campistrous et al 2005, Fountoulakis et al 2003, Molloy et al 2000) Identified in nucleoids collected by sucrose gradient centrifugation and analyzed by liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS). PMID: 21541338 Potential Skp substrate PMID: 19953543 Identified SurA substrates PMID: 19953543 Identified DsbA substrate PMID: 19953543 PMID: 9298646 SecA is sufficient to transport a secretory protein with hydrophobic signal sequence across SecYEG, but efficient translocation requires SRP in Addition PMID: 24717922 Disulfide bridge (311 323) Disulfide bond-containing protein PMID: 25664544
References:
PMID: 10806384 PMID: 16079137 PMID: 19953543 PMID: 20932056 PMID: 21778229 PMID: 24717922 PMID: 7813480 PMID: 9298646 PMID: 9808047
Pseudogene/Mobile Element:
-

mRNA Expression

mRNA abundance (Taniguchi 2010)

mRNA molec/cell:
-

Protein Existence

Mass spectrometry experiments

Proteomics:
PMID: 16606699 PMID: 12624733 PMID: 12488465 PMID: 17137328 PMID: 19348461 PMID: 20222674 PMID: 19003857 PMID: 16967446 PMID: 16858726 PMID: 19767571 PMID: 18727129 PMID: 16079137 PMID: 15115803 PMID: 20932056 PMID: 19343722 PMID: 17446557 PMID: 18796603 PMID: 20923772 PMID: 18425732
Proteomics / Inner Membrane fraction:
PMID: 16079137 PMID: 17446557 PMID: 17309111

Protein abundance/solubility

Protein abundance (Taniguchi 2010)

Protein molec/cell:
-
PaxDb:
2767 ppm

show distribution

EcoCyc

Protein:
EG10669
Gene Locus:
ompA

Protein ID (Uniprot)

Accession (Uniprot)

Gene Name (Uniprot)

Order Locus Name

Protein Name (STEPdb)

Full Name

Symbol

Uniprot

EchoLOCATION

Bernsel et al

Extracellular Appendage

First Level Targeting Chaperoning

First Level Secretion System

Second Level Targeting Chaperoning

Second Level Secretion System

K-12 Core Proteome (yes/no)

K-12 Basic Proteome (yes/no)

OMPA_ECOLI

P0A910

ompA

con tolG tut

b0957 JW0940

OM b-barrel protein (SEC through IM, inserted in OM by BAM), OmpA

Outer Membrane b-barrel protein

Cell outer membrane

Outer Membrane ??-barrel protein (Experimental)

Soluble protein detected in Inner Membrane Fraction

SEC

SKP,SURA,DEGP,DSBA

BAM: β-Barrel Assembly Machinery

yes

E. coli K-12 / MG1655/ ATCC 47076 proteome

Sub-cellular topologies of E.coli K-12(MG1655) proteins

2800

Manual Curation in STEP

mRNA Expression

Protein Existence

Protein abundance/solubility

Structural Annotation

Protein Family Domains

Protein Hydrodynamics

Bioinformatic tools and predictions