E. coli K-12 / MG1655/ ATCC 47076 proteome

UniProt Name:
Flagellar FliJ protein
Localization:
Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)
Notes:
Part of FlgN-FlgK-FliJ-FlhA complex.
References:
PMID: 22233518
Pseudogene/Mobile Element:
-

mRNA molec/cell:
-

Proteomics:
PMID: 16606699 PMID: 20222674
Proteomics / Inner Membrane fraction:
-

Protein molec/cell:
-
PaxDb:
0.836 ppm

Class:
-
Fold:
-
Super Family:
-

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UniProt Name:
Flagella synthesis protein FlgN
Localization:
-
Notes:
Substrate-specific chaperone of the flagellar export system along with FliT and FliS. Protects FlgK, FlgL from proteolysis. Interaction with FlhA which is an integral inner membrane protein isInvolved in efficient protein export.
References:
-
Pseudogene/Mobile Element:
-

mRNA molec/cell:
-

Proteomics:
PMID: 20923772
Proteomics / Inner Membrane fraction:
-

Protein molec/cell:
-
PaxDb:
109 ppm

Class:
All alpha proteins
SCOP: 46456
Fold:
STAT-like SCOP: 47654
Super Family:
FlgN-like
SCOP: 140566

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UniProt Name:
Negative regulator of flagellin synthesis (Anti-sigma-28 factor)
Localization:
Cytoplasmic, Extra-cellular (Probable)
Notes:
Anti-sigma28 factor. FlgM is an anti-sigma factor that binds and inactivates sigma28 (FliA_Ecoli). Once the hook and basal body is assembled, FlgM is secreted by the flagellar export system thereby freeing sigma28 to activate transcription.
References:
PMID: 9988479
Pseudogene/Mobile Element:
-

mRNA molec/cell:
-

Proteomics:
PMID: 16606699 PMID: 20222674 PMID: 20803007
Proteomics / Inner Membrane fraction:
-

Protein molec/cell:
-
PaxDb:
251 ppm

Class:
All alpha proteins
SCOP: 46456
Fold:
Non-globular all-alpha subunits of globular proteins
SCOP: 48661
Super Family:
Anti-sigma factor FlgM
SCOP: 101498

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UniProt Name:
Flagellar hook-associated protein 1 (HAP1)
Localization:
Peripheral outer membrane protein facing the extra-cellular space (Experimental) Peripheral outer membrane protein facing the extra-cellular space (Probable)
Notes:
Part of FlgN-FlgK-FliJ-FlhA complex. First hook-filament junction protein. Flagella proteins contribute to the production of outer membrane vesicles. Analysis of E. coli W3110 OMVs revealed that they contain abundant levels of FliC. Compared to the parental strain expressing wild-type flgK, an E. coli strain with a flgK-null mutation produced reduced amounts of OMVs. PMID: 12730325
References:
PMID: 12730325 PMID: 24134841
Pseudogene/Mobile Element:
-

mRNA molec/cell:
-

Proteomics:
PMID: 16606699 PMID: 19767571 PMID: 17446557
Proteomics / Inner Membrane fraction:
-

Protein molec/cell:
-
PaxDb:
56.2 ppm

Class:
Multi-domain proteins (alpha and beta)
SCOP: 56572
Fold:
Phase 1 flagellin SCOP: 64517
Super Family:
Phase 1 flagellin
SCOP: 64518

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UniProt Name:
Flagellar basal body rod protein FlgB (Putative proximal rod protein)
Localization:
Periplasmic (By similarity) Periplasmic (Experimental)
Notes:
Proximal rod protein. PMID: 12730325 FlgG is a distal component of the basal body rod. While FlgF was highly soluble, FlgB, FlgC, FlgG and FliE tended to self or cross-aggregate into fibrils in solutions at neutral pH or below, at high ionic strength, or at high protein concentration. PMID: 15136044
References:
PMID: 12631703 PMID: 12730325 PMID: 15136044
Pseudogene/Mobile Element:
-

mRNA molec/cell:
-

Proteomics:
PMID: 12488465 PMID: 20222674 PMID: 17446557
Proteomics / Inner Membrane fraction:
-

Protein molec/cell:
-
PaxDb:
0.696 ppm

Class:
-
Fold:
-
Super Family:
-

Go to Complexes

UniProt Name:
Flagellar basal-body rod protein FlgC (Putative proximal rod protein)
Localization:
Periplasmic (By similarity) Periplasmic (Experimental)
Notes:
Proximal rod protein. PMID: 12730325 FlgB, FlgC, FlgF, and FlgG are four proteins that comprise the rod section of the basal-body assembly of the flagellar motor. The rod is a rotational component which is situated transverse to the outer membrane and peptidoglycan layers and is circumscribed by a set of four rings which lie in a horizontal plane within the inner and outer membranes of the cell envelope. PMID: 2181149 FlgG is a distal component of the basal body rod. While FlgF was highly soluble, FlgB, FlgC, FlgG and FliE tended to self or cross-aggregate into fibrils in solutions at neutral pH or below, at high ionic strength, or at high protein concentration. PMID: 15136044
References:
PMID: 12631703 PMID: 12730325 PMID: 15136044 PMID: 2181149
Pseudogene/Mobile Element:
-

mRNA molec/cell:
-

Proteomics:
PMID: 16606699 PMID: 19767571
Proteomics / Inner Membrane fraction:
-

Protein molec/cell:
-
PaxDb:
0 ppm

Class:
-
Fold:
-
Super Family:
-

Go to Complexes

UniProt Name:
Flagellar basal-body rod protein FlgF (Putative proximal rod protein)
Localization:
Periplasmic (By similarity) Periplasmic (Experimental)
Notes:
Homolog to flgG. Proximal rod protein. PMID: 12730325 FlgB, FlgC, FlgF, and FlgG are four proteins that comprise the rod section of the basal-body assembly of the flagellar motor. The rod is a rotational component which is situated transverse to the outer membrane and peptidoglycan layers and is circumscribed by a set of four rings which lie in a horizontal plane within the inner and outer membranes of the cell envelope. PMID: 2181149 FlgG is a distal component of the basal body rod. While FlgF was highly soluble, FlgB, FlgC, FlgG and FliE tended to self or cross-aggregate into fibrils in solutions at neutral pH or below, at high ionic strength, or at high protein concentration. PMID: 15136044
References:
PMID: 12730325 PMID: 15136044 PMID: 2181149
Pseudogene/Mobile Element:
-

mRNA molec/cell:
-

Proteomics:
-
Proteomics / Inner Membrane fraction:
-

Protein molec/cell:
-
PaxDb:
0 ppm

Class:
All beta proteins
SCOP: 48724
Fold:
Flagellar hook protein flgE SCOP: 117142
Super Family:
Flagellar hook protein flgE
SCOP: 117143

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UniProt Name:
Flagellar basal-body rod protein FlgG (Distal rod protein)
Localization:
Periplasmic (By similarity) Periplasmic (Experimental)
Notes:
Distal rod protein PMID: 12730325 FlgB, FlgC, FlgF, and FlgG are four proteins that comprise the rod section of the basal-body assembly of the flagellar motor. The rod is a rotational component which is situated transverse to the outer membrane and peptidoglycan layers and is circumscribed by a set of four rings which lie in a horizontal plane within the inner and outer membranes of the cell envelope. PMID: 2181149 FlgG is a distal component of the basal body rod. While FlgF was highly soluble, FlgB, FlgC, FlgG and FliE tended to self or cross-aggregate into fibrils in solutions at neutral pH or below, at high ionic strength, or at high protein concentration. PMID: 15136044
References:
PMID: 12631703 PMID: 12730325 PMID: 15136044 PMID: 2181149
Pseudogene/Mobile Element:
-

mRNA molec/cell:
-

Proteomics:
PMID: 19767571
Proteomics / Inner Membrane fraction:
-

Protein molec/cell:
-
PaxDb:
5.58 ppm

Class:
All beta proteins
SCOP: 48724
Fold:
Flagellar hook protein flgE
SCOP: 117142
Super Family:
Flagellar hook protein flgE
SCOP: 117143

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UniProt Name:
Flagellar biosynthesis protein FlhA
Localization:
Integral Inner Membrane (Probable)
Notes:
Part of FlgN-FlgK-FliJ-FlhA complex.
References:
PMID: 15919996
Pseudogene/Mobile Element:
-

mRNA molec/cell:
-

Proteomics:
PMID: 16606699 PMID: 20222674 PMID: 19767571
Proteomics / Inner Membrane fraction:
-

Protein molec/cell:
-
PaxDb:
5.36 ppm

Class:
-
Fold:
-
Super Family:
-

Orientation on the IM:
in/in (N-terminus/C-terminus)

Go to Complexes