E. coli K-12 / MG1655/ ATCC 47076 proteome

UniProt Name:
ADP-ribose pyrophosphatase (EC 3.6.1.13) (ADP-ribose diphosphatase) (ADP-ribose phosphohydrolase) (ASPPase) (Adenosine diphosphoribose pyrophosphatase) (ADPR-PPase)
Localization:
Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)
Notes:
-
References:
PMID: 19766000
Pseudogene/Mobile Element:
-

mRNA molec/cell:
-

Proteomics:
PMID: 16606699 PMID: 20222674 PMID: 16858726 PMID: 19767571 PMID: 20923772
Proteomics / Inner Membrane fraction:
PMID: 17309111

Protein molec/cell:
-
PaxDb:
101 ppm

Class:
Alpha and beta proteins (a+b)
SCOP: 53931
Fold:
Nudix SCOP: 55810
Super Family:
Nudix
SCOP: 55811

Go to Complexes

UniProt Name:
Aerobic glycerol-3-phosphate dehydrogenase (EC 1.1.5.3)
Localization:
Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)
Notes:
Binds to the cytoplasmic membrane through an amphipathic C-terminal alpha-helix (357-374)PMID: 11955283. Identified in PlsB-PflB-GlpD-Psd PMID: 20521846. Detergent-depleted enzyme can be functionally reconstituted with Escherichia coli membrane vesicles to support glycerol-3-phosphate-dependent active transport of L-prolinePMID: 340460. When GlpD is lost by mutation, the cells not only fail to grow on glycerol or l-alpha-glycerophosphate, but are subject to growth inhibition in the presence of either compound PMID: 5321485. Glycerol-P dehydrogenase is a primary dehydrogenase in the electron transport chain. It functions in vitro only when associated with the cytoplasmic membrane. The enzyme has been purified from the membrane fraction after solubilization with deoxycholate. PMID: 1987111 GlpD enzyme is membrane bound. The active site has been located at the cytoplasmic (inner) face of the membrane. PMID: 6387427 Identified in the inner membrane by biochemical fractionation followed by two-dimensional gel electrophoresis and tandem mass spectrometry. PMID: 15911532 PMID: 8982270 Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). PMID: 23230279
References:
PMID: 11955283 PMID: 15911532 PMID: 15919657 PMID: 23230279 PMID: 340460 PMID: 8982270
Pseudogene/Mobile Element:
-

mRNA molec/cell:
0

Proteomics:
PMID: 16606699 PMID: 12624733 PMID: 12488465 PMID: 17137328 PMID: 19348461 PMID: 20222674 PMID: 16967446 PMID: 16858726 PMID: 19767571 PMID: 20521846 PMID: 15919657 PMID: 15115803 PMID: 20932056
Proteomics / Inner Membrane fraction:
PMID: 15919657 PMID: 17309111

Protein molec/cell:
70
PaxDb:
294 ppm

Class:
Alpha and beta proteins (a+b)
SCOP: 53931
Fold:
FAD/NAD(P)-binding domain
SCOP: 51904
FAD-linked reductases, C-terminal domain
SCOP: 54372
Super Family:
FAD/NAD(P)-binding domain
SCOP: 51905
FAD-linked reductases, C-terminal domain
SCOP: 54373

Go to Complexes

UniProt Name:
Alanine racemase, catabolic (EC 5.1.1.1)
Localization:
Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)
Notes:
-
References:
PMID: 23230279
Pseudogene/Mobile Element:
-

mRNA molec/cell:
3

Proteomics:
PMID: 20222674 PMID: 19767571 PMID: 15115803
Proteomics / Inner Membrane fraction:
-

Protein molec/cell:
10
PaxDb:
33.5 ppm

Class:
Alpha and beta proteins (a/b)
SCOP: 51349
Fold:
TIM beta/alpha-barrel SCOP: 51350
Super Family:
Alanine racemase C-terminal domain-like
SCOP: 50621
PLP-binding barrel
SCOP: 51419

Go to Complexes

UniProt Name:
Alcohol dehydrogenase YqhD (EC 1.1.1.-)
Localization:
Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)
Notes:
-
References:
PMID: 23230279
Pseudogene/Mobile Element:
-

mRNA molec/cell:
-

Proteomics:
PMID: 19348461 PMID: 20222674 PMID: 16858726 PMID: 19767571 PMID: 15115803
Proteomics / Inner Membrane fraction:
-

Protein molec/cell:
5
PaxDb:
192 ppm

Class:
Multi-domain proteins (alpha and beta)
SCOP: 56572
Fold:
Dehydroquinate synthase-like SCOP: 56795
Super Family:
Dehydroquinate synthase-like
SCOP: 56796

Go to Complexes

UniProt Name:
Alcohol dehydrogenase, propanol-preferring (EC 1.1.1.1)
Localization:
Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Potential)
Notes:
No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius.
References:
-
Pseudogene/Mobile Element:
-

mRNA molec/cell:
-

Proteomics:
PMID: 16606699 PMID: 20222674
Proteomics / Inner Membrane fraction:
-

Protein molec/cell:
-
PaxDb:
155 ppm

Class:
Alpha and beta proteins (a/b)
SCOP: 51349
Fold:
NAD(P)-binding Rossmann-fold domains SCOP: 51734
Super Family:
GroES-like
SCOP: 50129
NAD(P)-binding Rossmann-fold domains
SCOP: 51735

Go to Complexes

UniProt Name:
Aldehyde reductase YahK (EC 1.1.1.2) (Zinc-dependent alcohol dehydrogenase YahK)
Localization:
Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Potential)
Notes:
No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). PMID: 23230279 Disulfide bond-containing protein PMID: 25664544
References:
PMID: 23230279
Pseudogene/Mobile Element:
-

mRNA molec/cell:
-

Proteomics:
PMID: 12488465 PMID: 19348461 PMID: 20222674 PMID: 16858726 PMID: 19767571 PMID: 15115803 PMID: 18796603
Proteomics / Inner Membrane fraction:
-

Protein molec/cell:
-
PaxDb:
928 ppm

Class:
Alpha and beta proteins (a/b)
SCOP: 51349
Fold:
NAD(P)-binding Rossmann-fold domains SCOP: 51734
Super Family:
GroES-like
SCOP: 50129
NAD(P)-binding Rossmann-fold domains
SCOP: 51735

Go to Complexes

UniProt Name:
Aldehyde-alcohol dehydrogenase [Includes: Alcohol dehydrogenase (ADH) (EC 1.1.1.1); Acetaldehyde dehydrogenase [acetylating] (ACDH) (EC 1.2.1.10); Pyruvate-formate-lyase deactivase (PFL deactivase)]
Localization:
Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)
Notes:
Trigger factor-sensitive aggregation. Experimentally detected by proteomics analysis of E.coli membrane fraction (Bernsel et al 2009). No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Potential peripherally associated protein facing the cytoplasm or integral inner membrane protein.
References:
PMID: 19766000
Pseudogene/Mobile Element:
-

mRNA molec/cell:
-

Proteomics:
PMID: 16606699 PMID: 12624733 PMID: 12488465 PMID: 17137328 PMID: 19348461 PMID: 20222674 PMID: 16967446 PMID: 16858726 PMID: 19767571 PMID: 15115803
Proteomics / Inner Membrane fraction:
PMID: 17309111

Protein molec/cell:
-
PaxDb:
1598 ppm

Class:
Multi-domain proteins (alpha and beta)
SCOP: 56572
Fold:
Dehydroquinate synthase-like SCOP: 56795
Super Family:
ALDH-like
SCOP: 53720
Dehydroquinate synthase-like
SCOP: 56796

Go to Complexes

UniProt Name:
Aliphatic sulfonates import ATP-binding protein SsuB (EC 3.6.3.-)
Localization:
-
Notes:
-
References:
-
Pseudogene/Mobile Element:
-

mRNA molec/cell:
-

Proteomics:
PMID: 16606699 PMID: 12488465 PMID: 20222674
Proteomics / Inner Membrane fraction:
-

Protein molec/cell:
0
PaxDb:
0.313 ppm

Class:
Alpha and beta proteins (a/b)
SCOP: 51349
Fold:
P-loop containing nucleoside triphosphate hydrolases SCOP: 52539
Super Family:
P-loop containing nucleoside triphosphate hydrolases
SCOP: 52540

Go to Complexes

UniProt Name:
Alkyl hydroperoxide reductase C (EC 1.11.1.15) (Alkyl hydroperoxide reductase protein C22) (Peroxiredoxin) (SCRP-23) (Sulfate starvation-induced protein 8) (SSI8) (Thioredoxin peroxidase)
Localization:
Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)
Notes:
Disulfide bridge (47 47;166 166) Disulfide bond-containing protein PMID: 25664544
References:
PMID: 23230279
Pseudogene/Mobile Element:
-

mRNA molec/cell:
-

Proteomics:
PMID: 16606699 PMID: 12624733 PMID: 12488465 PMID: 17137328 PMID: 19348461 PMID: 20222674 PMID: 16967446 PMID: 16858726 PMID: 19767571 PMID: 20803007 PMID: 20521846 PMID: 15115803 PMID: 20932056
Proteomics / Inner Membrane fraction:
-

Protein molec/cell:
-
PaxDb:
3351 ppm

Class:
Alpha and beta proteins (a/b)
SCOP: 51349
Fold:
Thioredoxin fold
SCOP: 52832
Super Family:
Thioredoxin-like
SCOP: 52833

Go to Complexes

UniProt Name:
Alkyl hydroperoxide reductase subunit F (EC 1.8.1.-) (Alkyl hydroperoxide reductase F52A protein)
Localization:
Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Potential) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)
Notes:
Experimentally detected by proteomics analysis of E.coli membrane fraction (Bernsel et al 2009). No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Potential peripherally associated protein facing the cytoplasm or integral inner membrane protein. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). PMID: 23230279 Disulfide bridge (345 348)
References:
PMID: 19766000 PMID: 23230279
Pseudogene/Mobile Element:
-

mRNA molec/cell:
-

Proteomics:
PMID: 16606699 PMID: 12624733 PMID: 19348461 PMID: 20222674 PMID: 16858726 PMID: 19767571 PMID: 15115803
Proteomics / Inner Membrane fraction:
PMID: 17309111

Protein molec/cell:
-
PaxDb:
268 ppm

Class:
Alpha and beta proteins (a/b)
SCOP: 51349
Fold:
Thioredoxin fold SCOP: 52832
Super Family:
FAD/NAD(P)-binding domain
SCOP: 51905
Thioredoxin-like
SCOP: 52833

Go to Complexes

UniProt Name:
Alpha-D-ribose 1-methylphosphonate 5-triphosphate synthase subunit PhnL (RPnTP synthase subunit PhnL) (EC 2.7.8.37)
Localization:
Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (By similarity)
Notes:
Homolog to import ATP-binding protein MalK. Homolog to import ATP-binding protein MalK.
References:
-
Pseudogene/Mobile Element:
-

mRNA molec/cell:
-

Proteomics:
PMID: 16606699
Proteomics / Inner Membrane fraction:
-

Protein molec/cell:
-
PaxDb:
1.29 ppm

Class:
Alpha and beta proteins (a/b)
SCOP: 51349
Fold:
P-loop containing nucleoside triphosphate hydrolases
SCOP: 52539
Super Family:
P-loop containing nucleoside triphosphate hydrolases
SCOP: 52540

Go to Complexes

UniProt Name:
Altronate dehydratase (EC 4.2.1.7) (D-altronate hydro-lyase)
Localization:
Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Potential)
Notes:
No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius.
References:
-
Pseudogene/Mobile Element:
-

mRNA molec/cell:
0

Proteomics:
PMID: 16606699 PMID: 20222674
Proteomics / Inner Membrane fraction:
-

Protein molec/cell:
2
PaxDb:
8.67 ppm

Class:
-
Fold:
-
Super Family:
-

Go to Complexes

UniProt Name:
Amidophosphoribosyltransferase (ATase) (EC 2.4.2.14) (Glutamine phosphoribosylpyrophosphate amidotransferase) (GPATase)
Localization:
Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)
Notes:
Experimentally detected by proteomics analysis of E.coli membrane fraction (Bernsel et al 2009). No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Potential peripherally associated protein facing the cytoplasm or integral inner membrane protein.
References:
PMID: 19766000
Pseudogene/Mobile Element:
-

mRNA molec/cell:
-

Proteomics:
PMID: 12488465 PMID: 19348461 PMID: 20222674 PMID: 16858726 PMID: 19767571 PMID: 15115803
Proteomics / Inner Membrane fraction:
PMID: 17309111

Protein molec/cell:
-
PaxDb:
97 ppm

Class:
Alpha and beta proteins (a+b)
SCOP: 53931
Fold:
PRTase-like
SCOP: 53270
Ntn hydrolase-like
SCOP: 56234
Super Family:
PRTase-like
SCOP: 53271
N-terminal nucleophile aminohydrolases (Ntn hydrolases)
SCOP: 56235

Go to Complexes

UniProt Name:
Uncharacterized amino-acid ABC transporter ATP-binding protein YhdZ
Localization:
-
Notes:
-
References:
-
Pseudogene/Mobile Element:
-

mRNA molec/cell:
-

Proteomics:
PMID: 16606699
Proteomics / Inner Membrane fraction:
-

Protein molec/cell:
-
PaxDb:
0 ppm

Class:
Alpha and beta proteins (a/b)
SCOP: 51349
Fold:
P-loop containing nucleoside triphosphate hydrolases SCOP: 52539
Super Family:
P-loop containing nucleoside triphosphate hydrolases
SCOP: 52540

Go to Complexes

UniProt Name:
Aminomethyltransferase (EC 2.1.2.10) (Glycine cleavage system T protein)
Localization:
Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)
Notes:
-
References:
PMID: 23230279
Pseudogene/Mobile Element:
-

mRNA molec/cell:
1

Proteomics:
PMID: 16606699 PMID: 12624733 PMID: 12488465 PMID: 19348461 PMID: 20222674 PMID: 16858726 PMID: 19767571 PMID: 15115803 PMID: 18796603
Proteomics / Inner Membrane fraction:
-

Protein molec/cell:
40
PaxDb:
144 ppm

Class:
Alpha and beta proteins (a+b)
SCOP: 53931
Fold:
Folate-binding domain SCOP: 103024
Super Family:
Aminomethyltransferase beta-barrel domain
SCOP: 101790
Folate-binding domain
SCOP: 103025

Go to Complexes

UniProt Name:
Aminopeptidase N (EC 3.4.11.2) (Alpha-aminoacylpeptide hydrolase)
Localization:
Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (By similarity) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)
Notes:
Homologous peptidases are membrane associated in mammals, some data propose that the enzyme may associate with the E.coli membrane. It is structurally classified with <a href="http://www.ebi.ac.uk/thornton-srv/databases/cgi-bin/enzymes/GetPage.pl?ec_number=3.4.11.2">EC 3.4.11.2</a> membrane alanyl aminopeptidases. However, the extent of membrane association needs further analysis as it is generally soluble in the cytoplasmic fraction of E.coli and other bacteria. PMID: 323010 Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). PMID: 23230279
References:
PMID: 23230279 PMID: 323010
Pseudogene/Mobile Element:
-

mRNA molec/cell:
4

Proteomics:
PMID: 16606699 PMID: 19348461 PMID: 20222674 PMID: 16967446 PMID: 19767571 PMID: 15115803
Proteomics / Inner Membrane fraction:
-

Protein molec/cell:
133
PaxDb:
305 ppm

Class:
Alpha and beta proteins (a+b)
SCOP: 53931
Fold:
Leukotriene A4 hydrolase N-terminal domain SCOP: 63736
Super Family:
Metalloproteases (zincins), catalytic domain
SCOP: 55486
Leukotriene A4 hydrolase N-terminal domain
SCOP: 63737

Go to Complexes

UniProt Name:
Anaerobic glycerol-3-phosphate dehydrogenase subunit A (G-3-P dehydrogenase) (EC 1.1.5.3)
Localization:
Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)
Notes:
Confirmed cytoplasmic membrane localization of GlpB. GlpB has been isolated from inner membrane preparations. Part of anaerobic glycerol-3-phosphate dehydrogenase composed of a catalytic GlpA/B dimer and of membrane bound GlpC.
References:
-
Pseudogene/Mobile Element:
-

mRNA molec/cell:
-

Proteomics:
PMID: 16606699 PMID: 12624733 PMID: 12488465 PMID: 19348461 PMID: 20222674 PMID: 16858726 PMID: 19767571 PMID: 15115803 PMID: 20932056
Proteomics / Inner Membrane fraction:
PMID: 17309111

Protein molec/cell:
-
PaxDb:
129 ppm

Class:
Alpha and beta proteins (a/b)
SCOP: 51349
Fold:
FAD/NAD(P)-binding domain SCOP: 51904
Super Family:
FAD/NAD(P)-binding domain
SCOP: 51905

Go to Complexes

UniProt Name:
Anaerobic glycerol-3-phosphate dehydrogenase subunit B (Anaerobic G-3-P dehydrogenase subunit B) (Anaerobic G3Pdhase B) (EC 1.1.5.3)
Localization:
Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (By similarity) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)
Notes:
Confirmed cytoplasmic membrane localization of GlpB. GlpB has been isolated from inner membrane preparations. Part of anaerobic glycerol-3-phosphate dehydrogenase composed of a catalytic GlpA/B dimer and of membrane bound GlpC. GlpD enzyme is membrane bound. The active site has been located at the cytoplasmic (inner) face of the membrane. PMID: 6387427 Glycerol-P dehydrogenase is a primary dehydrogenase in the electron transport chain. It functions in vitro only when associated with the cytoplasmic membrane. The enzyme has been purified from the membrane fraction after solubilization with deoxycholate. PMID: 1987111 PMCID: PMC3210124 PMID: 3286606
References:
PMID: 1987111 PMID: 20706981 PMID: 3286606 PMID: 6387427 PMCID: PMC3210124
Pseudogene/Mobile Element:
-

mRNA molec/cell:
-

Proteomics:
PMID: 12624733 PMID: 12488465 PMID: 19348461 PMID: 20222674 PMID: 19767571 PMID: 15115803
Proteomics / Inner Membrane fraction:
-

Protein molec/cell:
-
PaxDb:
61.7 ppm

Class:
Alpha and beta proteins (a/b)
SCOP: 51349
Fold:
FAD/NAD(P)-binding domain
SCOP: 51904
Super Family:
FAD/NAD(P)-binding domain
SCOP: 51905

Go to Complexes

UniProt Name:
Anaerobic glycerol-3-phosphate dehydrogenase subunit C (G-3-P dehydrogenase)
Localization:
Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)
Notes:
Confirmed cytoplasmic membrane localization of GlpB. GlpB has been isolated from inner membrane preparations. Part of anaerobic glycerol-3-phosphate dehydrogenase composed of a catalytic GlpA/B dimer and of membrane bound GlpC. PMID: 3286606
References:
PMID: 3286606
Pseudogene/Mobile Element:
-

mRNA molec/cell:
-

Proteomics:
PMID: 16606699 PMID: 12624733 PMID: 12488465 PMID: 19348461 PMID: 20222674 PMID: 16858726 PMID: 15115803 PMID: 20932056
Proteomics / Inner Membrane fraction:
PMID: 17309111

Protein molec/cell:
0
PaxDb:
39.4 ppm

Class:
All alpha proteins
SCOP: 46456
Fold:
Globin-like SCOP: 46457
Super Family:
alpha-helical ferredoxin
SCOP: 46548

Go to Complexes

UniProt Name:
Anthranilate synthase component 1 (AS) (ASI) (EC 4.1.3.27)
Localization:
Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)
Notes:
Experimentally detected by proteomics analysis of E.coli membrane fraction (Bernsel et al 2009). No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Potential peripherally associated protein facing the cytoplasm or integral inner membrane protein.
References:
PMID: 19766000
Pseudogene/Mobile Element:
-

mRNA molec/cell:
-

Proteomics:
PMID: 16606699 PMID: 20222674
Proteomics / Inner Membrane fraction:
PMID: 17309111

Protein molec/cell:
-
PaxDb:
194 ppm

Class:
Alpha and beta proteins (a+b)
SCOP: 53931
Fold:
ADC synthase
SCOP: 56321
Super Family:
ADC synthase
SCOP: 56322

Go to Complexes