E. coli K-12 / MG1655/ ATCC 47076 proteome

UniProt Name:
Curli production assembly/transport component CsgF
Localization:
Peripheral outer membrane protein facing the extra-cellular space (Experimental) Peripheral outer membrane protein facing the extra-cellular space (Probable)
Notes:
Curli component. CsgF is secreted to the cell surface, where it mediates the cell-association and protease-resistance of the CsgB nucleator, suggesting that CsgF is required for specific localization and/or chaperoning of CsgB for full nucleator activity. Curli are a functional amyloid whose in vivo polymerization requires a dedicated nucleator protein, CsgB, and an assembly protein, CsgF. Without CsgF, curli subunits are released from the cell into the media and are inefficiently polymerized, resulting in fewer and mislocalized curli fibers. PMID: 19131513 PMID: 9928475
References:
PMID: 16522795 PMID: 19131513 PMID: 21645131 PMID: 9928475
Pseudogene/Mobile Element:
-

mRNA molec/cell:
-

Proteomics:
-
Proteomics / Inner Membrane fraction:
-

Protein molec/cell:
-
PaxDb:
0 ppm

Class:
-
Fold:
-
Super Family:
-

Go to Complexes

UniProt Name:
Major curlin subunit
Localization:
Peripheral outer membrane protein facing the extra-cellular space (Experimental) Peripheral outer membrane protein facing the extra-cellular space (Probable)
Notes:
Curli component. CsgA, is secreted from the cell in an unpolymerized form PMID: 11823641 The secretion and stability of CsgA and CsgB are dependent on the outer membrane lipoprotein CsgG. PMID: 21645131 Curli are a functional amyloid whose in vivo polymerization requires a dedicated nucleator protein, CsgB, and an assembly protein, CsgF. Without CsgF, curli subunits are released from the cell into the media and are inefficiently polymerized, resulting in fewer and mislocalized curli fibers. PMID: 19131513 PMID: 9383186 PMID: 9312041 During a process called interbacterial complementation, a csgB mutant cell secretes soluble CsgA that can be assembled on the surface of a cell expressing only csgB PMID: 8817489 High levels of both IbsC and ShoB lead to changes in membrane potential suggesting that both act at the membrane, consistent with the hydrophobic nature of the proteins.
References:
PMID: 11823641 PMID: 18710431 PMID: 21645131 PMID: 8817489 PMID: 9312041 PMID: 9383186
Pseudogene/Mobile Element:
-

mRNA molec/cell:
-

Proteomics:
-
Proteomics / Inner Membrane fraction:
-

Protein molec/cell:
-
PaxDb:
0 ppm

Class:
-
Fold:
-
Super Family:
-

Go to Complexes

UniProt Name:
Minor curlin subunit
Localization:
Peripheral outer membrane protein facing the extra-cellular space (Experimental) Peripheral outer membrane protein facing the extra-cellular space (Probable)
Notes:
Curli component. CsgB localization requires at least one component of the curli secretion apparatus, CsgF. Curli are a functional amyloid whose in vivo polymerization requires a dedicated nucleator protein, CsgB, and an assembly protein, CsgF. Without CsgF, curli subunits are released from the cell into the media and are inefficiently polymerized, resulting in fewer and mislocalized curli fibers. PMID: 19131513 PMID: 9383186 PMID: 9312041
References:
PMID: 19131513 PMID: 9312041 PMID: 9383186
Pseudogene/Mobile Element:
-

mRNA molec/cell:
-

Proteomics:
PMID: 19348461
Proteomics / Inner Membrane fraction:
-

Protein molec/cell:
-
PaxDb:
0.693 ppm

Class:
-
Fold:
-
Super Family:
-

Go to Complexes

UniProt Name:
Curli production assembly/transport component CsgG
Localization:
Outer Membrane Lipoprotein, Peripheral outer membrane protein facing the extra-cellular space (Experimental) Outer Membrane Lipoprotein, Peripheral outer membrane protein facing the extra-cellular space (Probable)
Notes:
Curli component. Proven signal peptidase II substrate. CsgG is an outer membrane protein required for the secretion of the two curli structural subunits CsgA and CsgB. CsgG is a lipoprotein that spans the outermembrane and assembles into an oligomeric annular-shaped structure with an inner diameter of approximately 2 nm (Margery L. Evans and Matthew R. Chapman in press). Overexpression of CsgG results in increased sensitivity to the small antibiotic erythromycin (740Da) but not the larger vancomycin (1440Da) suggesting that CsgG has a pore-like activity (Robinson et al 2006). PMID: 15174130
References:
PMID: 15174130 PMID: 21645131
Pseudogene/Mobile Element:
-

mRNA molec/cell:
1

Proteomics:
PMID: 17595003
Proteomics / Inner Membrane fraction:
-

Protein molec/cell:
2
PaxDb:
22.4 ppm

Class:
-
Fold:
-
Super Family:
-

Go to Complexes

UniProt Name:
Curli production assembly/transport component CsgE
Localization:
Periplasmic (Experimental)
Notes:
Potential substrate of periplasmic peptidyl prolyl cis???trans isomerase SurA; sequence contains known binding motif of SurA [RYEF]X[RYEF][P]. Curli secretion specificity factor. CsgE provides substrate specificity to the curli secretion pore CsgG, and acts directly on the secretion substrate CsgA to prevent premature subunit assembly. PMID: 9928475
References:
PMID: 9928475
Pseudogene/Mobile Element:
-

mRNA molec/cell:
-

Proteomics:
-
Proteomics / Inner Membrane fraction:
-

Protein molec/cell:
-
PaxDb:
0 ppm

Class:
-
Fold:
-
Super Family:
-

Go to Complexes

UniProt Name:
Secretion monitor
Localization:
Cytoplasmic, Periplasmic (Experimental) Cytoplasmic, Periplasmic (Probable)
Notes:
SecM regulates secA expression by translational coupling of the secM secA operon. Fusion of SecM with alkaline phosphatase activity exhibits substantial activity in the periplasm. SecM periplasmic form is rapidly degraded. Potential substrate of periplasmic peptidyl prolyl cis???trans isomerase SurA; sequence contains known binding motif of SurA [RYEF]X[RYEF][P]. PMID: 11597451
References:
PMID: 11597451 PMID: 1834634
Pseudogene/Mobile Element:
-

mRNA molec/cell:
-

Proteomics:
PMID: 20222674 PMID: 20803007
Proteomics / Inner Membrane fraction:
-

Protein molec/cell:
-
PaxDb:
0.338 ppm

Class:
-
Fold:
-
Super Family:
-

Go to Complexes

UniProt Name:
tRNA-modifying protein YgfZ
Localization:
Cytoplasmic, Periplasmic (By similarity) Cytoplasmic, Periplasmic (Experimental)
Notes:
Subcellular localization analysis of cytotoxic necrotizing factor 1 <a href="http://www.uniprot.org/uniprot/Q1R2U0">(CNF1)</a> demonstrated that YgfZ, a periplasmic protein, contributes to secretion of CNF1 (a normally cytoplasmic protein) into outer-membrane vesicles (OMVs) in pathogenic E.coli strain RS218 O18:K1:H7 PMID: 22174383. PMID: 9298646
References:
PMID: 22174383 PMID: 9298646
Pseudogene/Mobile Element:
-

mRNA molec/cell:
-

Proteomics:
PMID: 16606699 PMID: 12624733 PMID: 12488465 PMID: 17137328 PMID: 19348461 PMID: 20222674 PMID: 19767571 PMID: 15115803
Proteomics / Inner Membrane fraction:
-

Protein molec/cell:
-
PaxDb:
372 ppm

Class:
Alpha and beta proteins (a+b)
SCOP: 53931
Fold:
Elongation factor/aminomethyltransferase common domain
SCOP: 50464
Folate-binding domain
SCOP: 103024
Super Family:
Aminomethyltransferase beta-barrel domain
SCOP: 101790
Folate-binding domain
SCOP: 103025

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UniProt Name:
Metalloprotease PmbA (EC 3.4.-.-) (Protein TldE)
Localization:
-
Notes:
-
References:
-
Pseudogene/Mobile Element:
-

mRNA molec/cell:
-

Proteomics:
PMID: 16606699 PMID: 12624733 PMID: 12488465 PMID: 19348461 PMID: 20222674 PMID: 19767571 PMID: 15115803
Proteomics / Inner Membrane fraction:
-

Protein molec/cell:
-
PaxDb:
217 ppm

Class:
Alpha and beta proteins (a+b)
SCOP: 53931
Fold:
Putative modulator of DNA gyrase, PmbA/TldD
SCOP: 111282
Super Family:
Putative modulator of DNA gyrase, PmbA/TldD
SCOP: 111283

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UniProt Name:
Metalloprotease TldD (EC 3.4.-.-)
Localization:
-
Notes:
-
References:
-
Pseudogene/Mobile Element:
-

mRNA molec/cell:
2

Proteomics:
PMID: 19348461 PMID: 20222674 PMID: 19767571 PMID: 15115803
Proteomics / Inner Membrane fraction:
-

Protein molec/cell:
89
PaxDb:
134 ppm

Class:
Alpha and beta proteins (a+b)
SCOP: 53931
Fold:
Putative modulator of DNA gyrase, PmbA/TldD
SCOP: 111282
Super Family:
Putative modulator of DNA gyrase, PmbA/TldD
SCOP: 111283

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UniProt Name:
Aerobic respiration control protein ArcA (Dye resistance protein)
Localization:
Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (Experimental) Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (Probable)
Notes:
-
References:
PMID: 18792681 PMID: 23138451 PMID: 23230279
Pseudogene/Mobile Element:
-

mRNA molec/cell:
-

Proteomics:
PMID: 16606699 PMID: 12624733 PMID: 12488465 PMID: 19348461 PMID: 20222674 PMID: 19767571 PMID: 15115803 PMID: 17446557 PMID: 20923772
Proteomics / Inner Membrane fraction:
-

Protein molec/cell:
-
PaxDb:
770 ppm

Class:
Alpha and beta proteins (a/b)
SCOP: 51349
Fold:
Flavodoxin-like SCOP: 52171
Super Family:
C-terminal effector domain of the bipartite response regulators
SCOP: 46894
CheY-like
SCOP: 52172

Go to Complexes

UniProt Name:
Alanine--tRNA ligase (EC 6.1.1.7) (Alanyl-tRNA synthetase) (AlaRS)
Localization:
Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (Experimental)
Notes:
Trigger factor-sensitive aggregation.
References:
PMID: 23138451 PMID: 23230279
Pseudogene/Mobile Element:
-

mRNA molec/cell:
9

Proteomics:
PMID: 16606699 PMID: 12488465 PMID: 19348461 PMID: 20222674 PMID: 16858726 PMID: 19767571 PMID: 15115803 PMID: 20932056
Proteomics / Inner Membrane fraction:
-

Protein molec/cell:
86
PaxDb:
412 ppm

Class:
Alpha and beta proteins (a+b)
SCOP: 53931
Fold:
Reductase/isomerase/elongation factor common domain
SCOP: 50412
RRF/tRNA synthetase additional domain-like
SCOP: 55185
Class II aaRS and biotin synthetases
SCOP: 55680
Putative anticodon-binding domain of alanyl-tRNA synthetase (AlaRS)
SCOP: 101352
Super Family:
Translation proteins
SCOP: 50447
ThrRS/AlaRS common domain
SCOP: 55186
Class II aaRS and biotin synthetases
SCOP: 55681
Putative anticodon-binding domain of alanyl-tRNA synthetase (AlaRS)
SCOP: 101353

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UniProt Name:
ATP-dependent RNA helicase RhlB (EC 3.6.4.13)
Localization:
Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (Experimental)
Notes:
-
References:
PMID: 18337249 PMID: 23230279 PMID: 24438330
Pseudogene/Mobile Element:
-

mRNA molec/cell:
9

Proteomics:
PMID: 16606699 PMID: 12624733 PMID: 12488465 PMID: 19348461 PMID: 20222674 PMID: 16967446 PMID: 19767571 PMID: 15115803 PMID: 20932056
Proteomics / Inner Membrane fraction:
-

Protein molec/cell:
41
PaxDb:
174 ppm

Class:
Alpha and beta proteins (a/b)
SCOP: 51349
Fold:
P-loop containing nucleoside triphosphate hydrolases SCOP: 52539
Super Family:
P-loop containing nucleoside triphosphate hydrolases
SCOP: 52540

Go to Complexes

UniProt Name:
Bifunctional protein PutA [Includes: Proline dehydrogenase (EC 1.5.5.2) (Proline oxidase); Delta-1-pyrroline-5-carboxylate dehydrogenase (P5C dehydrogenase) (EC 1.2.1.88) (L-glutamate gamma-semialdehyde dehydrogenase)]
Localization:
Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (By similarity) Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (Experimental)
Notes:
The proline dehydrogenase PutA protein from S. typhimurium was shown to act as a membrane-associated enzyme or as a transcriptional repressor PMID: 8483946. Catabolism of proline requires PutA to associate with the membrane because reoxidation of the FAD cofactor in PutA needs the presence of an electron acceptor. Repression of the put operon requires PutA to bind to the put control-region DNA in the cytoplasm. Transcription factor PMID: 23138451. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). PMID: 23230279
References:
PMID: 23138451 PMID: 23230279 PMID: 8483946
Pseudogene/Mobile Element:
-

mRNA molec/cell:
0

Proteomics:
PMID: 16606699 PMID: 12624733 PMID: 12488465 PMID: 19348461 PMID: 20222674 PMID: 19767571 PMID: 15115803 PMID: 20932056
Proteomics / Inner Membrane fraction:
PMID: 17309111

Protein molec/cell:
12
PaxDb:
42.4 ppm

Class:
Alpha and beta proteins (a/b)
SCOP: 51349
Fold:
N-terminal domain of bifunctional PutA protein SCOP: 81934
Super Family:
Ribbon-helix-helix
SCOP: 47598
FAD-linked oxidoreductase
SCOP: 51730
ALDH-like
SCOP: 53720
N-terminal domain of bifunctional PutA protein
SCOP: 81935

Go to Complexes

UniProt Name:
cAMP-activated global transcriptional regulator CRP (Catabolite activator protein) (CAP) (Catabolite gene activator) (cAMP receptor protein) (CRP) (cAMP regulatory protein)
Localization:
Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (Experimental) Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (Probable)
Notes:
Trigger factor-sensitive aggregation.
References:
PMID: 15650111 PMID: 23138451 PMID: 23230279
Pseudogene/Mobile Element:
-

mRNA molec/cell:
-

Proteomics:
PMID: 12624733 PMID: 12488465 PMID: 19348461 PMID: 20222674 PMID: 19767571 PMID: 20803007 PMID: 15115803 PMID: 20932056
Proteomics / Inner Membrane fraction:
-

Protein molec/cell:
-
PaxDb:
726 ppm

Class:
All beta proteins
SCOP: 48724
Fold:
DNA/RNA-binding 3-helical bundle
SCOP: 46688
Double-stranded beta-helix
SCOP: 51181
Super Family:
Winged helix DNA-binding domain
SCOP: 46785
cAMP-binding domain-like
SCOP: 51206

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UniProt Name:
Chromosomal replication initiator protein DnaA
Localization:
Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (Experimental)
Notes:
C-terminal amphiphilic helix (355-370). Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). PMID: 23230279
References:
PMID: 10660626 PMID: 23138451 PMID: 23230279
Pseudogene/Mobile Element:
-

mRNA molec/cell:
7

Proteomics:
PMID: 16606699 PMID: 19348461 PMID: 20222674 PMID: 19767571 PMID: 15115803 PMID: 20932056
Proteomics / Inner Membrane fraction:
-

Protein molec/cell:
27
PaxDb:
31.3 ppm

Class:
Alpha and beta proteins (a/b)
SCOP: 51349
Fold:
P-loop containing nucleoside triphosphate hydrolases SCOP: 52539
Super Family:
TrpR-like
SCOP: 48295
P-loop containing nucleoside triphosphate hydrolases
SCOP: 52540

Go to Complexes

UniProt Name:
Chromosome partition protein MukB (Structural maintenance of chromosome-related protein)
Localization:
Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (Experimental) Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (Probable)
Notes:
-
References:
PMID: 11886550 PMID: 16982609 PMID: 20921377 PMID: 22380631 PMID: 23230279
Pseudogene/Mobile Element:
-

mRNA molec/cell:
-

Proteomics:
PMID: 16606699 PMID: 12488465 PMID: 19348461 PMID: 20222674 PMID: 16967446 PMID: 19767571 PMID: 15115803 PMID: 20932056
Proteomics / Inner Membrane fraction:
-

Protein molec/cell:
37
PaxDb:
64.8 ppm

Class:
Alpha and beta proteins (a/b)
SCOP: 51349
Fold:
P-loop containing nucleoside triphosphate hydrolases
SCOP: 52539
Super Family:
P-loop containing nucleoside triphosphate hydrolases
SCOP: 52540

Go to Complexes

UniProt Name:
Chromosome partition protein MukE (Protein KicA)
Localization:
Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (Experimental)
Notes:
-
References:
PMID: 16982609 PMID: 20921377 PMID: 23230279
Pseudogene/Mobile Element:
-

mRNA molec/cell:
-

Proteomics:
PMID: 16606699 PMID: 12488465 PMID: 19348461 PMID: 20222674 PMID: 19767571 PMID: 15115803
Proteomics / Inner Membrane fraction:
-

Protein molec/cell:
-
PaxDb:
103 ppm

Class:
-
Fold:
-
Super Family:
-

Go to Complexes

UniProt Name:
Chromosome partition protein MukF (Protein KicB)
Localization:
Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (Experimental)
Notes:
-
References:
PMID: 16982609 PMID: 20921377 PMID: 23230279
Pseudogene/Mobile Element:
-

mRNA molec/cell:
1

Proteomics:
PMID: 16606699 PMID: 12488465 PMID: 20222674 PMID: 19767571 PMID: 15115803
Proteomics / Inner Membrane fraction:
-

Protein molec/cell:
11
PaxDb:
22.1 ppm

Class:
All alpha proteins
SCOP: 46456
Fold:
STAT-like SCOP: 47654
Super Family:
Winged helix DNA-binding domain
SCOP: 46785
MukF C-terminal domain-like
SCOP: 140570

Go to Complexes

UniProt Name:
CsgBAC operon transcriptional regulatory protein
Localization:
Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (Experimental) Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (Potential)
Notes:
Necessary for transcription of the csgBAC/ymdA operon. Plays a positive role in biofilm formation. CsgD despite its function as a transcription activator, is localized in the cytoplasmic membrane. LuxR-type HTH domain signature and profile. The luxR-type HTH domain is a DNA-binding, helix-turn-helix (HTH) domain of about 65 amino acids, present in transcription regulators of the LuxR/FixJ family of response regulators (PROSITE: PDOC0054).
References:
PMID: 16513732 PMID: 20874755 PMID: 23138451
Pseudogene/Mobile Element:
-

mRNA molec/cell:
4

Proteomics:
PMID: 16606699 PMID: 20222674 PMID: 16967446
Proteomics / Inner Membrane fraction:
-

Protein molec/cell:
23
PaxDb:
48.4 ppm

Class:
All alpha proteins
SCOP: 46456
Fold:
DNA/RNA-binding 3-helical bundle SCOP: 46688
Super Family:
C-terminal effector domain of the bipartite response regulators
SCOP: 46894

Go to Complexes

UniProt Name:
DNA gyrase subunit A (EC 5.99.1.3)
Localization:
Cytoplasmic, Nucleoid, Peripheral inner membrane protein facing the cytoplasm (Experimental)
Notes:
Trigger factor-sensitive aggregation.
References:
PMID: 23230279
Pseudogene/Mobile Element:
-

mRNA molec/cell:
6

Proteomics:
PMID: 16606699 PMID: 12488465 PMID: 19348461 PMID: 20222674 PMID: 16858726 PMID: 19767571 PMID: 15115803 PMID: 20932056
Proteomics / Inner Membrane fraction:
-

Protein molec/cell:
68
PaxDb:
330 ppm

Class:
Multi-domain proteins (alpha and beta)
SCOP: 56572
Fold:
6-bladed beta-propeller
SCOP: 50938
Type II DNA topoisomerase
SCOP: 56718
Super Family:
Type II DNA topoisomerase
SCOP: 56719
GyrA/ParC C-terminal domain-like
SCOP: 101904

Go to Complexes