Disulfideome of E.coli K-12 (MG1655) proteome

UniProt Name:
Alkaline phosphatase (APase) (EC 3.1.3.1)
Localization:
Periplasmic (Experimental) Periplasmic (Probable)
Notes:
Substrate of periplasmic disulfide oxidoreductases DsbA. Alkaline phosphatase (EC 3.1.3.1; APase) of E.coli is a periplasmic enzyme. PMID: 3533724 Alkaline phosphatase (EC 3.1.3.1; APase) of E.coli is a periplasmic enzyme. PMID: 3533724 Identified DsbA substrate PMID: 19953543 PMID: 7022451 An amber mutation in the secA gene of Escherichia coli causes a pleiotropic decrease in the synthesis of secreted proteins. PMID: 3009404 Disulfide bridges (190 200;308 358) Disulfide bridges (190 200;308 358) Disulfide bond-containing protein PMID: 25664544
References:
PMID: 3009404 PMID: 3533724 PMID: 7022451
Pseudogene/Mobile Element:
-

mRNA molec/cell:
-

Proteomics:
PMID: 16606699 PMID: 19348461 PMID: 20222674 PMID: 19767571 PMID: 18425732
Proteomics / Inner Membrane fraction:
-

Protein molec/cell:
-
PaxDb:
19.6 ppm

Class:
Alpha and beta proteins (a/b)
SCOP: 51349
Fold:
Alkaline phosphatase-like
SCOP: 53648
Super Family:
Alkaline phosphatase-like
SCOP: 53649

Go to Complexes

UniProt Name:
L-asparaginase 2 (EC 3.5.1.1) (L-asparaginase II) (L-ASNase II) (L-asparagine amidohydrolase II) (Colaspase)
Localization:
Periplasmic (Experimental)
Notes:
-
References:
PMID: 2407723 PMID: 25403562 PMID: 387570 PMID: 8434007
Pseudogene/Mobile Element:
-

mRNA molec/cell:
-

Proteomics:
PMID: 16606699 PMID: 12624733 PMID: 12488465 PMID: 19348461 PMID: 20222674 PMID: 16858726 PMID: 19767571 PMID: 20521846 PMID: 15115803
Proteomics / Inner Membrane fraction:
-

Protein molec/cell:
-
PaxDb:
119 ppm

Class:
Alpha and beta proteins (a/b)
SCOP: 51349
Fold:
Glutaminase/Asparaginase
SCOP: 53773
Super Family:
Glutaminase/Asparaginase
SCOP: 53774

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UniProt Name:
Leucine-specific-binding protein (L-BP) (LS-BP)
Localization:
Periplasmic (Experimental)
Notes:
Identified DsbA substrate PMID: 19953543 PMID: 9298646 Potential substrate for the SRP pathway. It was found to be down-regulated in the periplasmic compartment of SRP-depleted cells. PMID: 22030350 Disulfide bridge (76 101) Disulfide bridge (42 47) PMID: 8061609 Disulfide bond-containing protein PMID: 25664544
References:
PMID: 22030350 PMID: 8061609 PMID: 9298646
Pseudogene/Mobile Element:
-

mRNA molec/cell:
-

Proteomics:
PMID: 16606699 PMID: 20222674
Proteomics / Inner Membrane fraction:
-

Protein molec/cell:
-
PaxDb:
360 ppm

Class:
Alpha and beta proteins (a/b)
SCOP: 51349
Fold:
Periplasmic binding protein-like I SCOP: 53821
Super Family:
Periplasmic binding protein-like I
SCOP: 53822

Go to Complexes

UniProt Name:
Protein UshA [Includes: UDP-sugar hydrolase (EC 3.6.1.45) (UDP-sugar diphosphatase) (UDP-sugar pyrophosphatase); 5'-nucleotidase (5'-NT) (EC 3.1.3.5)]
Localization:
Periplasmic (Experimental)
Notes:
-
References:
PMID: 9298646
Pseudogene/Mobile Element:
-

mRNA molec/cell:
-

Proteomics:
PMID: 16606699 PMID: 12624733 PMID: 12488465 PMID: 20222674 PMID: 19767571 PMID: 15115803 PMID: 17446557
Proteomics / Inner Membrane fraction:
PMID: 17309111

Protein molec/cell:
-
PaxDb:
122 ppm

Class:
Alpha and beta proteins (a+b)
SCOP: 53931
Fold:
Metallo-dependent phosphatases SCOP: 56299
Super Family:
5'-nucleotidase (syn. UDP-sugar hydrolase), C-terminal domain
SCOP: 55816
Metallo-dependent phosphatases
SCOP: 56300

Go to Complexes

UniProt Name:
Periplasmic AppA protein [Includes: Phosphoanhydride phosphohydrolase (EC 3.1.3.2) (pH 2.5 acid phosphatase) (AP); 4-phytase (EC 3.1.3.26)]
Localization:
Periplasmic (Experimental)
Notes:
Identified DsbC substrate PMID: 19953543 PMID: 8387749 Disulfide bridge (99 130;155 430;200 210;404 413) Disulfide bond-containing protein PMID: 25664544
References:
PMID: 8387749
Pseudogene/Mobile Element:
-

mRNA molec/cell:
-

Proteomics:
PMID: 16606699 PMID: 12624733 PMID: 20222674
Proteomics / Inner Membrane fraction:
-

Protein molec/cell:
-
PaxDb:
5.57 ppm

Class:
Alpha and beta proteins (a/b)
SCOP: 51349
Fold:
Phosphoglycerate mutase-like SCOP: 53253
Super Family:
Phosphoglycerate mutase-like
SCOP: 53254

Go to Complexes

UniProt Name:
2',3'-cyclic-nucleotide 2'-phosphodiesterase/3'-nucleotidase (EC 3.1.3.6) (EC 3.1.4.16)
Localization:
Periplasmic (Experimental) Periplasmic (Probable)
Notes:
Potential substrate of periplasmic peptidyl prolyl cis???trans isomerase SurA; sequence contains known binding motif of SurA [RYEF]X[RYEF][P]. References: Identified in periplasm by biochemical fractionation followed by two-dimensional gel electrophoresis and tandem mass spectrometry. PMID: 15911532 PMID: 9298646 Disulfide bond-containing protein PMID: 25664544
References:
PMID: 15911532 PMID: 3005231 PMID: 9298646
Pseudogene/Mobile Element:
-

mRNA molec/cell:
-

Proteomics:
PMID: 16606699 PMID: 12624733 PMID: 20222674 PMID: 19767571 PMID: 15115803 PMID: 18796603 PMID: 18425732
Proteomics / Inner Membrane fraction:
-

Protein molec/cell:
-
PaxDb:
91.6 ppm

Class:
Alpha and beta proteins (a+b)
SCOP: 53931
Fold:
Metallo-dependent phosphatases SCOP: 56299
Super Family:
5'-nucleotidase (syn. UDP-sugar hydrolase), C-terminal domain
SCOP: 55816
Metallo-dependent phosphatases
SCOP: 56300

Go to Complexes

UniProt Name:
Lysine/arginine/ornithine-binding periplasmic protein (LAO-binding protein)
Localization:
Periplasmic (Experimental)
Notes:
This periplasmic binding protein is involved in an arginine transport system. ArgT and histidine-binding protein J (hisJ) interact with a common membrane-bound receptor, hisP. Phosphorylated by ArgKPMID: 2136858. Identified DsbA substrate PMID: 19953543 PMID: 9298647 PMID: 9298646 Disulfide bridge (60 67) Disulfide bond-containing protein PMID: 25664544
References:
PMID: 9298646 PMID: 9298647
Pseudogene/Mobile Element:
-

mRNA molec/cell:
3

Proteomics:
PMID: 19348461 PMID: 20222674 PMID: 18727129 PMID: 15115803 PMID: 18796603 PMID: 18425732
Proteomics / Inner Membrane fraction:
-

Protein molec/cell:
1
PaxDb:
2133 ppm

Class:
Alpha and beta proteins (a/b)
SCOP: 51349
Fold:
Periplasmic binding protein-like II SCOP: 53849
Super Family:
Periplasmic binding protein-like II
SCOP: 53850

Go to Complexes

UniProt Name:
Flagellar P-ring protein (Basal body P-ring protein)
Localization:
Periplasmic (Experimental)
Notes:
P ring protein. Substrate of periplasmic disulfide oxidoreductases DsbA. Might be is co-translationally translocated through Sec translocon. Identified DsbA substrate PMID: 19953543 PMID: 12730325 Potential substrate for the SRP pathway. When fused to cytoplasmic protein thioredoxin promotes signal recognition particle-dependent translocation PMID: 15838024
References:
PMID: 12730325 PMID: 15838024
Pseudogene/Mobile Element:
-

mRNA molec/cell:
-

Proteomics:
PMID: 20222674
Proteomics / Inner Membrane fraction:
-

Protein molec/cell:
-
PaxDb:
0.45 ppm

Class:
-
Fold:
-
Super Family:
-

Go to Complexes

UniProt Name:
Uncharacterized protein YbgS
Localization:
-
Notes:
Disulfide bond-containing protein PMID: 25664544
References:
-
Pseudogene/Mobile Element:
-

mRNA molec/cell:
-

Proteomics:
PMID: 20222674 PMID: 19767571 PMID: 15115803
Proteomics / Inner Membrane fraction:
-

Protein molec/cell:
-
PaxDb:
606 ppm

Class:
-
Fold:
-
Super Family:
-

Go to Complexes

UniProt Name:
Iron uptake system component EfeO
Localization:
Periplasmic (Experimental) Periplasmic (Probable)
Notes:
Identified DsbA substrate PMID: 19953543 PMID: 12107143 Potential substrate for the SRP pathway. It was found to be down-regulated in the periplasmic compartment of SRP-depleted cells. PMID: 22030350 Disulfide bond-containing protein PMID: 25664544
References:
PMID: 12107143 PMID: 16551627 PMID: 22030350
Pseudogene/Mobile Element:
-

mRNA molec/cell:
2

Proteomics:
PMID: 16606699 PMID: 19348461 PMID: 20222674 PMID: 18727129 PMID: 18425732
Proteomics / Inner Membrane fraction:
-

Protein molec/cell:
5
PaxDb:
96.1 ppm

Class:
All beta proteins
SCOP: 48724
Fold:
Cupredoxin-like SCOP: 49502
Super Family:
Cupredoxins
SCOP: 49503

Go to Complexes

UniProt Name:
Uncharacterized protein YmgD
Localization:
-
Notes:
Disulfide bond-containing protein PMID: 25664544
References:
-
Pseudogene/Mobile Element:
-

mRNA molec/cell:
-

Proteomics:
PMID: 16606699 PMID: 20222674 PMID: 20803007 PMID: 15115803
Proteomics / Inner Membrane fraction:
-

Protein molec/cell:
-
PaxDb:
15.9 ppm

Class:
-
Fold:
-
Super Family:
-

Go to Complexes

UniProt Name:
Cytochrome c-552 (EC 1.7.2.2) (Ammonia-forming cytochrome c nitrite reductase) (Cytochrome c nitrite reductase)
Localization:
Periplasmic (Experimental)
Notes:
Disulfide bond-containing protein PMID: 25664544
References:
PMID: 793493
Pseudogene/Mobile Element:
-

mRNA molec/cell:
-

Proteomics:
PMID: 16606699 PMID: 19348461 PMID: 20222674
Proteomics / Inner Membrane fraction:
-

Protein molec/cell:
-
PaxDb:
17.7 ppm

Class:
All alpha proteins
SCOP: 46456
Fold:
Multiheme cytochromes
SCOP: 48694
Super Family:
Multiheme cytochromes
SCOP: 48695

Go to Complexes

UniProt Name:
Cytochrome c-type protein NrfB
Localization:
Periplasmic (Experimental)
Notes:
Disulfide bond-containing protein PMID: 25664544
References:
PMID: 15280383
Pseudogene/Mobile Element:
-

mRNA molec/cell:
-

Proteomics:
PMID: 19348461 PMID: 20222674
Proteomics / Inner Membrane fraction:
-

Protein molec/cell:
-
PaxDb:
7.69 ppm

Class:
All alpha proteins
SCOP: 46456
Fold:
Multiheme cytochromes
SCOP: 48694
Super Family:
Multiheme cytochromes
SCOP: 48695

Go to Complexes

UniProt Name:
Periplasmic nitrate reductase, electron transfer subunit (Diheme cytochrome c NapB)
Localization:
Periplasmic (Experimental) Periplasmic (Probable)
Notes:
Nitrate reductase, napFDAGHBC, is located in the periplasm. E. coli also expresses two membrane-bound proton-translocating nitrate reductases, encoded by the narGHJI and narZYWV operons. PMID: 11844760 PMID: 10234835 Disulfide bond-containing protein PMID: 25664544
References:
PMID: 10234835 PMID: 10548535 PMID: 11844760 PMID: 17130127
Pseudogene/Mobile Element:
-

mRNA molec/cell:
-

Proteomics:
PMID: 20222674 PMID: 20803007
Proteomics / Inner Membrane fraction:
-

Protein molec/cell:
-
PaxDb:
0 ppm

Class:
All alpha proteins
SCOP: 46456
Fold:
Multiheme cytochromes
SCOP: 48694
Super Family:
Multiheme cytochromes
SCOP: 48695

Go to Complexes

UniProt Name:
Uncharacterized fimbrial-like protein SfmA (Type-1A pilin)
Localization:
-
Notes:
Potential fimbrial sfm operon (class ??1); Fimbrial Subunit. Disulfide bridge (41 81)
References:
-
Pseudogene/Mobile Element:
-

mRNA molec/cell:
-

Proteomics:
-
Proteomics / Inner Membrane fraction:
-

Protein molec/cell:
-
PaxDb:
0 ppm

Class:
All beta proteins
SCOP: 48724
Fold:
Common fold of diphtheria toxin/transcription factors/cytochrome f
SCOP: 49379
Super Family:
Bacterial adhesins
SCOP: 49401

Go to Complexes

UniProt Name:
Uncharacterized protein YdcA
Localization:
-
Notes:
Disulfide bond-containing protein PMID: 25664544
References:
-
Pseudogene/Mobile Element:
-

mRNA molec/cell:
-

Proteomics:
PMID: 16606699 PMID: 20222674
Proteomics / Inner Membrane fraction:
-

Protein molec/cell:
-
PaxDb:
0 ppm

Class:
-
Fold:
-
Super Family:
-

Go to Complexes

UniProt Name:
Inhibitor of vertebrate lysozyme
Localization:
Periplasmic (Experimental)
Notes:
Experimentally verified gene-product. Verified that the signal peptide is cleaved. PMID: 11278658 Disulfide bridge (85 90) Disulfide bond-containing protein PMID: 25664544
References:
PMID: 11278658
Pseudogene/Mobile Element:
-

mRNA molec/cell:
-

Proteomics:
PMID: 12488465 PMID: 20222674 PMID: 20803007 PMID: 15115803 PMID: 18425732
Proteomics / Inner Membrane fraction:
PMID: 17309111

Protein molec/cell:
-
PaxDb:
495 ppm

Class:
Alpha and beta proteins (a+b)
SCOP: 53931
Fold:
Inhibitor of vertebrate lysozyme, Ivy
SCOP: 89871
Super Family:
Inhibitor of vertebrate lysozyme, Ivy
SCOP: 89872

Go to Complexes

UniProt Name:
Leu/Ile/Val-binding protein (LIV-BP)
Localization:
Periplasmic (Experimental)
Notes:
Substrate of periplasmic disulfide oxidoreductases DsbA. Identified DsbA substrate PMID: 19953543 PMID: 9298646 Potential substrate for the SRP pathway. It was found to be down-regulated in the periplasm and up-regulated in the cytoplasm of SRP-depleted cells. PMID: 22030350 Disulfide bridge (76 101) Disulfide bond-containing protein PMID: 25664544
References:
PMID: 22030350 PMID: 9298646
Pseudogene/Mobile Element:
-

mRNA molec/cell:
4

Proteomics:
PMID: 16606699 PMID: 20222674 PMID: 18425732
Proteomics / Inner Membrane fraction:
-

Protein molec/cell:
8
PaxDb:
2975 ppm

Class:
Alpha and beta proteins (a/b)
SCOP: 51349
Fold:
Periplasmic binding protein-like I
SCOP: 53821
Super Family:
Periplasmic binding protein-like I
SCOP: 53822

Go to Complexes

UniProt Name:
Uncharacterized protein YggN
Localization:
Periplasmic (Experimental)
Notes:
Substrate of periplasmic disulfide oxidoreductases DsbA. Identified DsbA substrate PMID: 19953543 PMID: 14726535
References:
PMID: 14726535
Pseudogene/Mobile Element:
-

mRNA molec/cell:
-

Proteomics:
PMID: 16606699 PMID: 19348461 PMID: 20222674 PMID: 19767571 PMID: 15115803 PMID: 19343722
Proteomics / Inner Membrane fraction:
-

Protein molec/cell:
-
PaxDb:
108 ppm

Class:
-
Fold:
-
Super Family:
-

Go to Complexes

UniProt Name:
Protein CreA (Catabolite regulation protein A)
Localization:
-
Notes:
Identified DsbA substrate PMID: 19953543 Disulfide bond-containing protein PMID: 25664544
References:
-
Pseudogene/Mobile Element:
-

mRNA molec/cell:
-

Proteomics:
PMID: 16606699 PMID: 20222674 PMID: 19767571 PMID: 20803007 PMID: 15115803
Proteomics / Inner Membrane fraction:
-

Protein molec/cell:
-
PaxDb:
49.7 ppm

Class:
-
Fold:
-
Super Family:
-

Go to Complexes