Disulfideome of E.coli K-12 (MG1655) proteome

UniProt Name:
Ribonucleoside-diphosphate reductase 1 subunit alpha (EC 1.17.4.1) (Protein B1) (Ribonucleoside-diphosphate reductase 1 R1 subunit) (Ribonucleotide reductase 1)
Localization:
Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)
Notes:
Trigger factor-sensitive aggregation. Disulfide bridges between 225 and 462 PMID: 9395490 Disulfide bridges between 225 and 462 PMID: 9395490 Disulfide bridges between 225 and 462 PMID: 9395490
References:
PMID: 23230279 PMID: 9395490
Pseudogene/Mobile Element:
-

mRNA molec/cell:
4

Proteomics:
PMID: 16606699 PMID: 12488465 PMID: 19348461 PMID: 20222674 PMID: 16967446 PMID: 19767571 PMID: 15115803 PMID: 20932056
Proteomics / Inner Membrane fraction:
-

Protein molec/cell:
251
PaxDb:
390 ppm

Class:
Alpha and beta proteins (a/b)
SCOP: 51349
Fold:
R1 subunit of ribonucleotide reductase, N-terminal domain
SCOP: 48167
PFL-like glycyl radical enzymes
SCOP: 51997
Super Family:
R1 subunit of ribonucleotide reductase, N-terminal domain
SCOP: 48168
PFL-like glycyl radical enzymes
SCOP: 51998

Go to Complexes

UniProt Name:
Bifunctional aspartokinase/homoserine dehydrogenase 2 (Aspartokinase II/homoserine dehydrogenase II) (AKII-HDII) [Includes: Aspartokinase (EC 2.7.2.4); Homoserine dehydrogenase (EC 1.1.1.3)]
Localization:
Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)
Notes:
Disulfide bond-containing protein PMID: 25664544
References:
PMID: 23230279
Pseudogene/Mobile Element:
-

mRNA molec/cell:
0

Proteomics:
PMID: 16606699 PMID: 19348461 PMID: 20222674 PMID: 19767571 PMID: 20932056
Proteomics / Inner Membrane fraction:
-

Protein molec/cell:
45
PaxDb:
73 ppm

Class:
Alpha and beta proteins (a+b)
SCOP: 53931
Fold:
NAD(P)-binding Rossmann-fold domains
SCOP: 51734
Carbamate kinase-like
SCOP: 53632
FwdE/GAPDH domain-like
SCOP: 55346
Super Family:
NAD(P)-binding Rossmann-fold domains
SCOP: 51735
Carbamate kinase-like
SCOP: 53633
Glyceraldehyde-3-phosphate dehydrogenase-like, C-terminal domain
SCOP: 55347

Go to Complexes

UniProt Name:
Isoleucine--tRNA ligase (EC 6.1.1.5) (Isoleucyl-tRNA synthetase) (IleRS)
Localization:
-
Notes:
Translation unit. Disulfide bond-containing protein PMID: 25664544
References:
-
Pseudogene/Mobile Element:
-

mRNA molec/cell:
4

Proteomics:
PMID: 16606699 PMID: 12488465 PMID: 19348461 PMID: 20222674 PMID: 19767571 PMID: 15115803 PMID: 20932056
Proteomics / Inner Membrane fraction:
-

Protein molec/cell:
64
PaxDb:
381 ppm

Class:
Alpha and beta proteins (a/b)
SCOP: 51349
Fold:
Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases
SCOP: 47322
ValRS/IleRS/LeuRS editing domain
SCOP: 50676
Adenine nucleotide alpha hydrolase-like
SCOP: 52373
Super Family:
Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases
SCOP: 47323
ValRS/IleRS/LeuRS editing domain
SCOP: 50677
Nucleotidylyl transferase
SCOP: 52374

Go to Complexes

UniProt Name:
Glycine--tRNA ligase alpha subunit (EC 6.1.1.14) (Glycyl-tRNA synthetase alpha subunit) (GlyRS)
Localization:
Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)
Notes:
Translation unit. Trigger factor-sensitive aggregation. Disulfide bond-containing protein PMID: 25664544
References:
PMID: 23230279
Pseudogene/Mobile Element:
-

mRNA molec/cell:
-

Proteomics:
PMID: 16606699 PMID: 12488465 PMID: 19348461 PMID: 20222674 PMID: 19767571 PMID: 15115803 PMID: 20932056
Proteomics / Inner Membrane fraction:
-

Protein molec/cell:
-
PaxDb:
306 ppm

Class:
Alpha and beta proteins (a+b)
SCOP: 53931
Fold:
Class II aaRS and biotin synthetases
SCOP: 55680
Super Family:
Class II aaRS and biotin synthetases
SCOP: 55681

Go to Complexes

UniProt Name:
Aromatic-amino-acid aminotransferase (ARAT) (AROAT) (EC 2.6.1.57) (Beta-methylphenylalanine transaminase) (EC 2.6.1.107)
Localization:
-
Notes:
Disulfide bond-containing protein PMID: 25664544
References:
-
Pseudogene/Mobile Element:
-

mRNA molec/cell:
-

Proteomics:
PMID: 16606699 PMID: 12624733 PMID: 12488465 PMID: 19348461 PMID: 20222674 PMID: 16967446 PMID: 19767571 PMID: 15115803
Proteomics / Inner Membrane fraction:
-

Protein molec/cell:
-
PaxDb:
211 ppm

Class:
Alpha and beta proteins (a/b)
SCOP: 51349
Fold:
PLP-dependent transferase-like SCOP: 53382
Super Family:
PLP-dependent transferases
SCOP: 53383

Go to Complexes

UniProt Name:
Thioredoxin/glutathione peroxidase BtuE (EC 1.11.1.15) (EC 1.11.1.9)
Localization:
-
Notes:
Disulfide bond-containing protein PMID: 25664544
References:
-
Pseudogene/Mobile Element:
-

mRNA molec/cell:
-

Proteomics:
PMID: 16606699 PMID: 12488465 PMID: 20222674 PMID: 19767571 PMID: 15115803
Proteomics / Inner Membrane fraction:
-

Protein molec/cell:
-
PaxDb:
536 ppm

Class:
Alpha and beta proteins (a/b)
SCOP: 51349
Fold:
Thioredoxin fold SCOP: 52832
Super Family:
Thioredoxin-like
SCOP: 52833

Go to Complexes

UniProt Name:
Glutathione reductase (GR) (GRase) (EC 1.8.1.7)
Localization:
Cytoplasmic (Experimental)
Notes:
Disulfide bridge (42 47) PMID: 8061609
References:
PMID: 8061609
Pseudogene/Mobile Element:
-

mRNA molec/cell:
1

Proteomics:
PMID: 16606699 PMID: 12624733 PMID: 12488465 PMID: 19348461 PMID: 20222674 PMID: 16858726 PMID: 19767571 PMID: 15115803
Proteomics / Inner Membrane fraction:
-

Protein molec/cell:
1
PaxDb:
110 ppm

Class:
Alpha and beta proteins (a+b)
SCOP: 53931
Fold:
CO dehydrogenase flavoprotein C-domain-like SCOP: 55423
Super Family:
FAD/NAD(P)-binding domain
SCOP: 51905
FAD/NAD-linked reductases, dimerisation (C-terminal) domain
SCOP: 55424

Go to Complexes

UniProt Name:
L-threonine 3-dehydrogenase (TDH) (EC 1.1.1.103) (L-threonine dehydrogenase)
Localization:
-
Notes:
Disulfide bond-containing protein PMID: 25664544
References:
-
Pseudogene/Mobile Element:
-

mRNA molec/cell:
-

Proteomics:
PMID: 16606699 PMID: 12488465 PMID: 19348461 PMID: 20222674 PMID: 16858726 PMID: 19767571 PMID: 15115803 PMID: 20932056
Proteomics / Inner Membrane fraction:
-

Protein molec/cell:
-
PaxDb:
80.2 ppm

Class:
Alpha and beta proteins (a/b)
SCOP: 51349
Fold:
NAD(P)-binding Rossmann-fold domains SCOP: 51734
Super Family:
GroES-like
SCOP: 50129
NAD(P)-binding Rossmann-fold domains
SCOP: 51735

Go to Complexes

UniProt Name:
Acetolactate synthase isozyme 1 large subunit (AHAS-I) (EC 2.2.1.6) (Acetohydroxy-acid synthase I large subunit) (ALS-I)
Localization:
-
Notes:
Disulfide bond-containing protein PMID: 25664544
References:
-
Pseudogene/Mobile Element:
-

mRNA molec/cell:
-

Proteomics:
PMID: 16606699 PMID: 20222674 PMID: 19767571 PMID: 15115803
Proteomics / Inner Membrane fraction:
-

Protein molec/cell:
14
PaxDb:
272 ppm

Class:
Alpha and beta proteins (a/b)
SCOP: 51349
Fold:
Thiamin diphosphate-binding fold (THDP-binding) SCOP: 52517
Super Family:
DHS-like NAD/FAD-binding domain
SCOP: 52467
Thiamin diphosphate-binding fold (THDP-binding)
SCOP: 52518

Go to Complexes

UniProt Name:
Dihydrofolate synthase/folylpolyglutamate synthase (DHFS / FPGS) (EC 6.3.2.12) (EC 6.3.2.17) (Folylpoly-gamma-glutamate synthetase-dihydrofolate synthetase) (Folylpolyglutamate synthetase) (Tetrahydrofolylpolyglutamate synthase)
Localization:
-
Notes:
Disulfide bridge (354 401)
References:
-
Pseudogene/Mobile Element:
-

mRNA molec/cell:
1

Proteomics:
PMID: 19348461 PMID: 20222674 PMID: 19767571 PMID: 15115803
Proteomics / Inner Membrane fraction:
-

Protein molec/cell:
15
PaxDb:
43.7 ppm

Class:
Alpha and beta proteins (a/b)
SCOP: 51349
Fold:
Ribokinase-like SCOP: 53612
Super Family:
MurD-like peptide ligases, peptide-binding domain
SCOP: 53244
MurD-like peptide ligases, catalytic domain
SCOP: 53623

Go to Complexes

UniProt Name:
Lysine-sensitive aspartokinase 3 (EC 2.7.2.4) (Aspartate kinase III) (AKIII) (Lysine-sensitive aspartokinase III)
Localization:
-
Notes:
Disulfide bond-containing protein PMID: 25664544
References:
-
Pseudogene/Mobile Element:
-

mRNA molec/cell:
-

Proteomics:
PMID: 12488465 PMID: 20222674 PMID: 19767571
Proteomics / Inner Membrane fraction:
-

Protein molec/cell:
-
PaxDb:
121 ppm

Class:
Alpha and beta proteins (a+b)
SCOP: 53931
Fold:
Ferredoxin-like SCOP: 54861
Super Family:
Carbamate kinase-like
SCOP: 53633
ACT-like
SCOP: 55021

Go to Complexes

UniProt Name:
Phosphoenolpyruvate-protein phosphotransferase (EC 2.7.3.9) (Phosphotransferase system, enzyme I)
Localization:
Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable)
Notes:
-
References:
PMID: 23230279 PMID: 2644649
Pseudogene/Mobile Element:
-

mRNA molec/cell:
-

Proteomics:
PMID: 16606699 PMID: 12624733 PMID: 12488465 PMID: 17137328 PMID: 19348461 PMID: 20222674 PMID: 16858726 PMID: 19767571 PMID: 15115803 PMID: 20932056
Proteomics / Inner Membrane fraction:
-

Protein molec/cell:
-
PaxDb:
1103 ppm

Class:
Alpha and beta proteins (a/b)
SCOP: 51349
Fold:
The swivelling beta/beta/alpha domain SCOP: 52008
Super Family:
Enzyme I of the PEP
sugar phosphotransferase system HPr-binding (sub)domain
SCOP: 47831
Phosphoenolpyruvate/pyruvate domain
SCOP: 51621
Phosphohistidine domain
SCOP: 52009

Go to Complexes

UniProt Name:
Glutamate synthase [NADPH] large chain (EC 1.4.1.13) (Glutamate synthase subunit alpha) (GLTS alpha chain) (NADPH-GOGAT)
Localization:
Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)
Notes:
Disulfide bond-containing protein PMID: 25664544
References:
PMID: 23230279
Pseudogene/Mobile Element:
-

mRNA molec/cell:
6

Proteomics:
PMID: 16606699 PMID: 12488465 PMID: 19348461 PMID: 20222674 PMID: 16967446 PMID: 19767571 PMID: 15115803
Proteomics / Inner Membrane fraction:
-

Protein molec/cell:
174
PaxDb:
302 ppm

Class:
Alpha and beta proteins (a+b)
SCOP: 53931
Fold:
Ntn hydrolase-like SCOP: 56234
Super Family:
FMN-linked oxidoreductases
SCOP: 51395
N-terminal nucleophile aminohydrolases (Ntn hydrolases)
SCOP: 56235
Alpha subunit of glutamate synthase, C-terminal domain
SCOP: 69336

Go to Complexes

UniProt Name:
3-isopropylmalate dehydratase large subunit (EC 4.2.1.33) (Alpha-IPM isomerase) (IPMI) (Isopropylmalate isomerase)
Localization:
-
Notes:
Disulfide bond-containing protein PMID: 25664544
References:
-
Pseudogene/Mobile Element:
-

mRNA molec/cell:
-

Proteomics:
PMID: 16606699 PMID: 20222674 PMID: 16967446
Proteomics / Inner Membrane fraction:
-

Protein molec/cell:
-
PaxDb:
374 ppm

Class:
Alpha and beta proteins (a/b)
SCOP: 51349
Fold:
Aconitase iron-sulfur domain SCOP: 53731
Super Family:
Aconitase iron-sulfur domain
SCOP: 53732

Go to Complexes

UniProt Name:
Diaminopimelate epimerase (DAP epimerase) (EC 5.1.1.7) (PLP-independent amino acid racemase)
Localization:
-
Notes:
Disulfide bridge (73 217)
References:
-
Pseudogene/Mobile Element:
-

mRNA molec/cell:
7

Proteomics:
PMID: 16606699 PMID: 12624733 PMID: 20222674 PMID: 19767571 PMID: 15115803
Proteomics / Inner Membrane fraction:
-

Protein molec/cell:
34
PaxDb:
69 ppm

Class:
Alpha and beta proteins (a+b)
SCOP: 53931
Fold:
Diaminopimelate epimerase-like SCOP: 54505
Super Family:
Diaminopimelate epimerase-like
SCOP: 54506

Go to Complexes

UniProt Name:
Glutamate--cysteine ligase (EC 6.3.2.2) (Gamma-ECS) (GCS) (Gamma-glutamylcysteine synthetase)
Localization:
-
Notes:
Disulfide bond-containing protein PMID: 25664544
References:
-
Pseudogene/Mobile Element:
-

mRNA molec/cell:
4

Proteomics:
PMID: 12488465 PMID: 19348461 PMID: 20222674 PMID: 19767571 PMID: 15115803
Proteomics / Inner Membrane fraction:
-

Protein molec/cell:
24
PaxDb:
78.2 ppm

Class:
Alpha and beta proteins (a+b)
SCOP: 53931
Fold:
Glutamine synthetase/guanido kinase SCOP: 55930
Super Family:
Glutamine synthetase/guanido kinase
SCOP: 55931

Go to Complexes

UniProt Name:
33 kDa chaperonin (Heat shock protein 33) (HSP33)
Localization:
Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)
Notes:
Disulfide bridge (230 232;263 266) PMID: 10764757
References:
PMID: 23230279
Pseudogene/Mobile Element:
-

mRNA molec/cell:
-

Proteomics:
PMID: 19348461 PMID: 20222674 PMID: 19767571 PMID: 15115803
Proteomics / Inner Membrane fraction:
-

Protein molec/cell:
81
PaxDb:
263 ppm

Class:
Small proteins
SCOP: 56992
Fold:
HSP33 redox switch-like SCOP: 118351
Super Family:
Hsp33 domain
SCOP: 64397
HSP33 redox switch-like
SCOP: 118352

Go to Complexes

UniProt Name:
Peptide methionine sulfoxide reductase MsrA (Protein-methionine-S-oxide reductase) (EC 1.8.4.11) (Peptide-methionine (S)-S-oxide reductase) (Peptide Met(O) reductase)
Localization:
Cytoplasmic (Experimental)
Notes:
YeaA has been viewed through fluorescence microscopy and located evenly throughout cytoplasm. Small scale study of labeling chromosomal coding DNA sequences (CDSs) with fluorescent protein genes. PMID: 17272300 Disulfide bridge (52 199); (199 207)
References:
PMID: 17272300
Pseudogene/Mobile Element:
-

mRNA molec/cell:
-

Proteomics:
PMID: 16606699 PMID: 20222674 PMID: 20803007
Proteomics / Inner Membrane fraction:
-

Protein molec/cell:
-
PaxDb:
292 ppm

Class:
Alpha and beta proteins (a+b)
SCOP: 53931
Fold:
Ferredoxin-like SCOP: 54861
Super Family:
Peptide methionine sulfoxide reductase
SCOP: 55068

Go to Complexes

UniProt Name:
Peptide methionine sulfoxide reductase MsrB (EC 1.8.4.12) (Peptide-methionine (R)-S-oxide reductase)
Localization:
Cytoplasmic (Experimental)
Notes:
Fluorescent fusions of YeaA have been monitored via fluorescence microscopy. YeaA was found to be located evenly throughout cytoplasm. Disulfide bond-containing protein PMID: 25664544
References:
PMID: 17272300
Pseudogene/Mobile Element:
-

mRNA molec/cell:
15

Proteomics:
PMID: 20222674 PMID: 19767571 PMID: 20803007 PMID: 15115803
Proteomics / Inner Membrane fraction:
-

Protein molec/cell:
60
PaxDb:
325 ppm

Class:
All beta proteins
SCOP: 48724
Fold:
Mss4-like SCOP: 51315
Super Family:
Mss4-like
SCOP: 51316

Go to Complexes

UniProt Name:
Glucosamine-6-phosphate deaminase (EC 3.5.99.6) (GlcN6P deaminase) (GNPDA) (Glucosamine-6-phosphate isomerase)
Localization:
Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental)
Notes:
Disulfide bridge (219 219) PMID: 8240271
References:
PMID: 23230279
Pseudogene/Mobile Element:
-

mRNA molec/cell:
-

Proteomics:
PMID: 16606699 PMID: 12488465 PMID: 19348461 PMID: 20222674 PMID: 16967446 PMID: 19767571 PMID: 15115803
Proteomics / Inner Membrane fraction:
-

Protein molec/cell:
-
PaxDb:
212 ppm

Class:
Alpha and beta proteins (a/b)
SCOP: 51349
Fold:
NagB/RpiA/CoA transferase-like SCOP: 100949
Super Family:
NagB/RpiA/CoA transferase-like
SCOP: 100950

Go to Complexes