| entry | acc | gene | alt_gene | oln | protein_name | cm_loca | cm_notes | cm_refs | nterm_cterm | suboci_full | subloci | subloci_iim | bernsel | subloci_uniprot | subloci_echoL | organelle | target_chaperoning1 | 1st_ss | target_chaperoning2 | 2nd_ss | pseudo | common_core | basic_proteome | signalp_sp_exist | signalp_pos | signalp_cmax | lipop_pred | lipop_cleavage | lipop_score | lipop_rule | tmhmm | phobius_tms | phobius_sp_exist | phobius_prediction | psortb_loca | psortb_score | solubility |
| RIR1_ECOLI | P00452 | nrdA dnaF | dnaF | b2234 JW2228 | cytoplasmic peripheral IM protein, Ribonucleoside-diphosphate reductase 1 subunit alpha NrdA | Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) | Trigger factor-sensitive aggregation. Disulfide bridges between 225 and 462 PMID: 9395490 Disulfide bridges between 225 and 462 PMID: 9395490 Disulfide bridges between 225 and 462 PMID: 9395490 | PMID: 23230279 PMID: 9395490 | Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm | A, F1 | A,F1 | N/A | Cytoplasmic | TF | yes | yes | N | 40 | 0.104 | CYT | 0 | -0.200913 | - | 0 | 0 | N | i | Cytoplasmic | 9 | 63 | |||||||
| AK2H_ECOLI | P00562 | metL metM | metM | b3940 JW3911 | cytoplasmic peripheral IM protein, Bifunctional aspartokinase/homoserine dehydrogenase 2 MetL | Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) | Disulfide bond-containing protein PMID: 25664544 | PMID: 23230279 | Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm | A, F1 | A,F1 | N/A | Cytoplasmic | yes | yes | N | 24 | 0.117 | CYT | 0 | -0.200913 | - | 0 | 0 | N | i | CytoplasmicMembrane | 9 | |||||||||
| PPB_ECOLI | P00634 | phoA | b0383 JW0374 | periplasmic protein (SEC through IM), Alkaline phosphatase PhoA | Periplasmic (Experimental) Periplasmic (Probable) | Substrate of periplasmic disulfide oxidoreductases DsbA. Alkaline phosphatase (EC 3.1.3.1; APase) of E.coli is a periplasmic enzyme. PMID: 3533724 Alkaline phosphatase (EC 3.1.3.1; APase) of E.coli is a periplasmic enzyme. PMID: 3533724 Identified DsbA substrate PMID: 19953543 PMID: 7022451 An amber mutation in the secA gene of Escherichia coli causes a pleiotropic decrease in the synthesis of secreted proteins. PMID: 3009404 Disulfide bridges (190 200;308 358) Disulfide bridges (190 200;308 358) Disulfide bond-containing protein PMID: 25664544 | PMID: 3009404 PMID: 3533724 PMID: 7022451 | Periplasmic | G | G | N/A | Periplasm | Periplasmic (Experimental) | SEC | DSBA | no | yes | Y | 22 | 0.408 | SpI | 21 | 3.05238 | - | 0 | 0 | Y | n5-16c21/22o | Periplasmic | 10 | 78 | ||||||
| LEP_ECOLI | P00803 | lepB | b2568 JW2552 | integral IM protein (SEC-YidC in IM), Signal peptidase I LepB | Integral Inner Membrane (Experimental) Integral Inner Membrane (Probable) | Fractionation analysis indicates that upon YidC depletion the protein membrane localization is significantly decreased. Lep was shown to be targeted to the membrane by the SRP pathway. PMID: 8985168 Disulfide bridge (171 177) | PMID: 15919996 PMID: 21778229 PMID: 8985168 | out/out | Integral Inner Membrane | B | B | IM (predicted) | Cell inner membrane | Integral Membrane Protein | SEC-YidC | yes | yes | N | 22 | 0.131 | SpII | 20 | 3.04951 | Pos+2=V | 2 | 0 | Y | n5-16c21/22o | CytoplasmicMembrane | 10 | 27 | ||||||
| ASPG2_ECOLI | P00805 | ansB | b2957 JW2924 | periplasmic protein (SEC through IM), L-asparaginase 2 AnsB | Periplasmic (Experimental) | PMID: 2407723 PMID: 25403562 PMID: 387570 PMID: 8434007 | Periplasmic | G | G | N/A | Periplasm | Periplasmic (Experimental) | SEC | yes | yes | Y | 23 | 0.82 | SpI | 22 | 19.7315 | - | 1 | 0 | Y | n7-17c22/23o | Periplasmic | 10 | 49 | ||||||||
| SYI_ECOLI | P00956 | ileS ilvS | ilvS | b0026 JW0024 | cytoplasmic protein, Isoleucine--tRNA ligase IleS | Translation unit. Disulfide bond-containing protein PMID: 25664544 | Cytoplasmic | A | A | N/A | Cytoplasm | Cytoplasmic | yes | yes | N | 61 | 0.113 | CYT | 0 | -0.200913 | - | 0 | 0 | N | i | Cytoplasmic | 10 | ||||||||||
| SYGA_ECOLI | P00960 | glyQ glyS(A) | glyS(A) | b3560 JW3531 | cytoplasmic peripheral IM protein, Glycine--tRNA ligase alpha subunit GlyQ | Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) | Translation unit. Trigger factor-sensitive aggregation. Disulfide bond-containing protein PMID: 25664544 | PMID: 23230279 | Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm | A, F1 | A,F1 | N/A | Cytoplasm | Cytoplasmic | TF | no | yes | N | 57 | 0.112 | CYT | 0 | -0.200913 | - | 0 | 0 | N | i | Cytoplasmic | 9 | 59 | ||||||
| LAMB_ECOLI | P02943 | lamB malB | malB | b4036 JW3996 | OM b-barrel protein (SEC through IM, inserted in OM by BAM), Maltoporin LamB | Outer Membrane b-barrel protein (Experimental) Outer Membrane b-barrel protein (Probable) | Potential pubstrate of periplasmic chaperones Skp. Substrate of periplasmic peptidyl prolyl cis???trans isomerase SurA. Outer membrane protein determined by 2D gel electrophoresis (Lopez-Campistrous et al 2005, Fountoulakis et al 2003, Molloy 2000). Experimentally verified trimeric beta-barrel. N-terminal region is in periplasm. Potential Skp substrate PMID: 19953543 Identified SurA substrates PMID: 19953543 PMID: 346375 Disulfide bridges between 47 and 63 PMID: 1988451 Disulfide bond-containing protein PMID: 25664544 | PMID: 10806384 PMID: 15911532 PMID: 16079137 PMID: 16372265 PMID: 1988451 PMID: 20932056 PMID: 346375 | Outer Membrane b-barrel protein | H | H | Soluble IM Fraction | Cell outer membrane | Outer Membrane ??-barrel protein (Experimental) | SEC | SKP,SURA,DEGP | BAM | yes | yes | Y | 26 | 0.795 | SpI | 25 | 20.7922 | - | 0 | 0 | Y | n8-18c25/26o | OuterMembrane | 10 | 54 | ||||
| FIMA1_ECOLI | P04128 | fimA pilA | pilA | b4314 JW4277 | Fimbrial extra-cellular peripheral OM protein (SEC through IM, chaperone usher export system over OM), Type-1 protein, A chain FimA | Peripheral outer membrane protein facing the extra-cellular space (Experimental) | Fim operon (class ??1); Fimbrial Subunit. Experimentally verified signal peptide PMID: 2858471 Disulfide bridge (44 84) Disulfide bond-containing protein PMID: 25664544 | PMID: 2858471 | Peripheral outer membrane protein facing the extra-cellular space | F4 | F4 | N/A | Fimbrium | Cell surface appendage (Experimental) | fimbriae | SEC | CU | yes | yes | Y | 24 | 0.78 | SpI | 23 | 25.7568 | - | 1 | 0 | Y | n5-16c23/24o | Extracellular | 10 | |||||
| TYRB_ECOLI | P04693 | tyrB | b4054 JW4014 | cytoplasmic protein, Aromatic-amino-acid aminotransferase TyrB | Disulfide bond-containing protein PMID: 25664544 | Cytoplasmic | A | A | N/A | Cytoplasm | Cytoplasmic | yes | yes | N | 10 | 0.122 | CYT | 0 | -0.200913 | - | 0 | 0 | N | i | Cytoplasmic | 10 | 38 | ||||||||||
| LIVK_ECOLI | P04816 | livK | b3458 JW3423 | periplasmic protein (SEC through IM), Leucine-specific-binding LivK | Periplasmic (Experimental) | Identified DsbA substrate PMID: 19953543 PMID: 9298646 Potential substrate for the SRP pathway. It was found to be down-regulated in the periplasmic compartment of SRP-depleted cells. PMID: 22030350 Disulfide bridge (76 101) Disulfide bridge (42 47) PMID: 8061609 Disulfide bond-containing protein PMID: 25664544 | PMID: 22030350 PMID: 8061609 PMID: 9298646 | Periplasmic | G | G | N/A | Periplasm | Periplasmic (Experimental) | SEC | DSBA | yes | yes | Y | 24 | 0.817 | SpI | 23 | 17.7516 | - | 0 | 0 | Y | n7-18c23/24o | Periplasmic | 10 | 78 | ||||||
| FEPA_ECOLI | P05825 | fepA fep feuB | fep feuB | b0584 JW5086 | OM b-barrel protein (SEC through IM, inserted in OM by BAM), Ferrienterobactin receptor FepA | Outer Membrane b-barrel protein (Experimental) Outer Membrane b-barrel protein (Probable) | Outer membrane protein determined by 2D gel electrophoresis (Lopez-Campistrous et al 2005, Fountoulakis et al 2003, Molloy et al 2000) Potential Skp substrate PMID: 19953543 PMID: 10529396 Disulfide bond-containing protein PMID: 25664544 | PMID: 10529396 PMID: 10806384 PMID: 11266632 PMID: 20932056 PMID: 9886293 | Outer Membrane b-barrel protein | H | H | N/A | Cell outer membrane; Multi-pass membrane protein | Outer Membrane ??-barrel protein (Experimental) | SEC | SKP | BAM | yes | yes | Y | 23 | 0.836 | SpI | 22 | 11.5401 | - | 0 | 0 | Y | n7-17c22/23o | OuterMembrane | 10 | 11 | ||||
| BTUE_ECOLI | P06610 | btuE | b1710 JW1700 | cytoplasmic protein, Thioredoxin/glutathione peroxidase BtuE | Disulfide bond-containing protein PMID: 25664544 | Cytoplasmic | A | A | N/A | Periplasm | Cytoplasmic | yes | yes | N | 37 | 0.111 | CYT | 0 | -0.200913 | - | 0 | 0 | N | i | Periplasmic | 10 | 99 | ||||||||||
| GSHR_ECOLI | P06715 | gor | b3500 JW3467 | cytoplasmic protein, Glutathione reductase Gor | Cytoplasmic (Experimental) | Disulfide bridge (42 47) PMID: 8061609 | PMID: 8061609 | Cytoplasmic | A | A | N/A | Cytoplasm | Cytoplasmic | yes | yes | N | 24 | 0.239 | CYT | 0 | -0.200913 | - | 0 | 0 | N | i | Cytoplasmic | 10 | 67 | ||||||||
| FHUA_ECOLI | P06971 | fhuA tonA | tonA | b0150 JW0146 | OM b-barrel protein (SEC through IM, inserted in OM by BAM), Ferrichrome transporter/phage receptor FhuA | Outer Membrane b-barrel protein (Experimental) Outer Membrane b-barrel protein (Probable) | SecB substrate; Substrate of periplasmic peptidyl prolyl cis???trans isomerase SurA. Outer membrane protein determined by 2D gel electrophoresis (Lopez-Campistrous et al 2005, Fountoulakis et al 2003, Molloy et al 2000) Potential Skp substrate PMID: 19953543 Identified SurA substrates PMID: 19953543 PMID: 3079747 Disulfide bridge (351 362;725 731) Disulfide bond-containing protein PMID: 25664544 | PMID: 10806384 PMID: 11266632 PMID: 15911532 PMID: 3079747 PMID: 9856937 | Outer Membrane b-barrel protein | H | H | N/A | Cell outer membrane | Outer Membrane ??-barrel protein (Experimental) | SECB | SEC | SURA,SKP | BAM | yes | yes | Y | 34 | 0.805 | SpI | 33 | 20.4987 | - | 0 | 0 | Y | n16-24c33/34o | OuterMembrane | 10 | 7 | |||
| USHA_ECOLI | P07024 | ushA | b0480 JW0469 | periplasmic protein (SEC through IM), UshA | Periplasmic (Experimental) | PMID: 9298646 | Periplasmic | G | G | Soluble IM Fraction | Periplasm | Periplasmic (Experimental) | SEC | no | yes | Y | 26 | 0.748 | SpI | 25 | 11.3752 | - | 0 | 0 | Y | n7-18c25/26o | Periplasmic | 10 | 77 | ||||||||
| PPA_ECOLI | P07102 | appA | b0980 JW0963 | periplasmic protein (SEC through IM), AppA | Periplasmic (Experimental) | Identified DsbC substrate PMID: 19953543 PMID: 8387749 Disulfide bridge (99 130;155 430;200 210;404 413) Disulfide bond-containing protein PMID: 25664544 | PMID: 8387749 | Periplasmic | G | G | N/A | Periplasm | Periplasmic (Experimental) | SEC | DSBC | yes | yes | Y | 23 | 0.793 | SpI | 22 | 13.6965 | - | 0 | 0 | Y | n3-13c22/23o | Periplasmic | 10 | 66 | ||||||
| TDH_ECOLI | P07913 | tdh | b3616 JW3591 | cytoplasmic protein, L-threonine 3-dehydrogenase Tdh | Disulfide bond-containing protein PMID: 25664544 | Cytoplasmic | A | A | IM (predicted) | Cytoplasm | Cytoplasmic | yes | yes | N | 25 | 0.103 | CYT | 0 | -0.200913 | - | 0 | 0 | N | i | Cytoplasmic | 10 | 50 | ||||||||||
| ILVB_ECOLI | P08142 | ilvB | b3671 JW3646 | cytoplasmic protein, Acetolactate synthase isozyme 1 large subunit IlvB | Disulfide bond-containing protein PMID: 25664544 | Cytoplasmic | A | A | N/A | Cytoplasmic | yes | yes | N | 45 | 0.118 | CYT | 0 | -0.200913 | - | 0 | 0 | N | i | Cytoplasmic | 9 | 29 | |||||||||||
| FIMF_ECOLI | P08189 | fimF | b4318 JW4281 | Fimbrial extra-cellular peripheral OM protein (SEC through IM, chaperone usher export system over OM), FimF | Peripheral outer membrane protein facing the extra-cellular space (Experimental) | Fim operon (class ??1); Fimbrial Subunit. Required for the regulation of length and mediation of adhesion of Escherichia coli type 1 fimbriae. PMID: 15372038 Disulfide bridge (38 78) Disulfide bond-containing protein PMID: 25664544 | PMID: 15372038 | Peripheral outer membrane protein facing the extra-cellular space | F4 | F4 | N/A | Fimbrium | Cell surface appendage (Experimental) | fimbriae | SEC | CU | no | yes | Y | 23 | 0.871 | SpI | 22 | 17.2366 | - | 0 | 0 | Y | n6-17c22/23o | Extracellular | 9 | ||||||
| FIMG_ECOLI | P08190 | fimG | b4319 JW4282 | Fimbrial extra-cellular peripheral OM protein (SEC through IM, chaperone usher export system over OM), FimG | Peripheral outer membrane protein facing the extra-cellular space (Experimental) | Fim operon (class ??1); Fimbrial Subunit. Required for the regulation of length and mediation of adhesion of Escherichia coli type 1 fimbriae. PMID: 15372038 Disulfide bridge (39 77) Disulfide bond-containing protein PMID: 25664544 | PMID: 15372038 | Peripheral outer membrane protein facing the extra-cellular space | F4 | F4 | N/A | Fimbrium | Cell surface appendage (Experimental) | fimbriae | SEC | CU | no | yes | Y | 24 | 0.773 | SpI | 23 | 18.1281 | - | 1 | 0 | Y | n7-18c23/24o | Extracellular | 9 | 73 | |||||
| FOLC_ECOLI | P08192 | folC dedC | dedC | b2315 JW2312 | cytoplasmic protein, Dihydrofolate synthase/folylpolyglutamate synthase FolC | Disulfide bridge (354 401) | Cytoplasmic | A | A | N/A | Cytoplasmic | yes | yes | N | 26 | 0.113 | CYT | 0 | -0.200913 | - | 0 | 0 | N | i | Cytoplasmic | 9 | 84 | ||||||||||
| CPDB_ECOLI | P08331 | cpdB | b4213 JW4171 | periplasmic protein (SEC through IM), 2',3'-cyclic-nucleotide 2'-phosphodiesterase/3'-nucleotidase CpdB | Periplasmic (Experimental) Periplasmic (Probable) | Potential substrate of periplasmic peptidyl prolyl cis???trans isomerase SurA; sequence contains known binding motif of SurA [RYEF]X[RYEF][P]. References: Identified in periplasm by biochemical fractionation followed by two-dimensional gel electrophoresis and tandem mass spectrometry. PMID: 15911532 PMID: 9298646 Disulfide bond-containing protein PMID: 25664544 | PMID: 15911532 PMID: 3005231 PMID: 9298646 | Periplasmic | G | G | N/A | Periplasm | Periplasmic (Experimental) | SEC | SURA | no | yes | Y | 20 | 0.787 | SpI | 19 | 14.5002 | - | 0 | 0 | Y | n4-14c19/20o | Periplasmic | 10 | 82 | ||||||
| AK3_ECOLI | P08660 | lysC apk | apk | b4024 JW3984 | cytoplasmic protein, Lysine-sensitive aspartokinase 3 LysC | Disulfide bond-containing protein PMID: 25664544 | Cytoplasmic | A | A | N/A | Cytoplasmic | yes | yes | N | 43 | 0.206 | CYT | 0 | -0.200913 | - | 0 | 0 | N | i | Unknown | 4 | |||||||||||
| PT1_ECOLI | P08839 | ptsI | b2416 JW2409 | cytoplasmic peripheral IM protein, Phosphoenolpyruvate-phosphotransferase PtsI | Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable) | PMID: 23230279 PMID: 2644649 | Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm | A, F1 | A,F1 | N/A | Cytoplasm | Cytoplasmic | yes | yes | N | 15 | 0.115 | CYT | 0 | -0.200913 | - | 0 | 0 | N | i | Cytoplasmic | 10 | 90 | |||||||||
| ARGT_ECOLI | P09551 | argT | b2310 JW2307 | periplasmic protein (SEC through IM), Lysine/arginine/ornithine-binding periplasmic ArgT | Periplasmic (Experimental) | This periplasmic binding protein is involved in an arginine transport system. ArgT and histidine-binding protein J (hisJ) interact with a common membrane-bound receptor, hisP. Phosphorylated by ArgKPMID: 2136858. Identified DsbA substrate PMID: 19953543 PMID: 9298647 PMID: 9298646 Disulfide bridge (60 67) Disulfide bond-containing protein PMID: 25664544 | PMID: 9298646 PMID: 9298647 | Periplasmic | G | G | N/A | Periplasm | Periplasmic (Experimental) | SEC | yes | yes | Y | 23 | 0.595 | SpI | 22 | 19.5206 | - | 0 | 0 | Y | n4-14c22/23o | Periplasmic | 10 | ||||||||
| GLTB_ECOLI | P09831 | gltB aspB | aspB | b3212 JW3179 | cytoplasmic peripheral IM protein, Glutamate synthase (NADPH) large chain GltB | Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) | Disulfide bond-containing protein PMID: 25664544 | PMID: 23230279 | Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm | A, F1 | A,F1 | N/A | Cytoplasmic | no | yes | N | 48 | 0.135 | CYT | 0 | -0.200913 | - | 0 | 0 | N | i | Cytoplasmic | 9 | |||||||||
| LEUC_ECOLI | P0A6A6 | leuC | b0072 JW0071 | cytoplasmic protein, 3-isopropylmalate dehydratase large subunit LeuC | Disulfide bond-containing protein PMID: 25664544 | Cytoplasmic | A | A | N/A | Cytoplasmic | no | yes | N | 41 | 0.123 | CYT | 0 | -0.200913 | - | 0 | 0 | N | i | Cytoplasmic | 9 | 70 | |||||||||||
| DAPF_ECOLI | P0A6K1 | dapF | b3809 JW5592 | cytoplasmic protein, Diaminopimelate epimerase DapF | Disulfide bridge (73 217) | Cytoplasmic | A | A | IM (predicted) | Cytoplasm | Cytoplasmic | yes | yes | N | 19 | 0.106 | CYT | 0 | -0.200913 | - | 0 | 0 | N | i | Cytoplasmic | 9 | 17 | ||||||||||
| DSBB_ECOLI | P0A6M2 | dsbB roxB ycgA | roxB ycgA | b1185 JW5182 | integral IM protein (SEC-YidC in IM), Disulfide bond formation B DsbB | Integral Inner Membrane (Experimental) Integral Inner Membrane (Probable) | Exhibites aberrant localization by microscopy in the absence of YidC PMID: 22108384. The crystal structure revealed a transmembrane (TM) four-helix-bundle structure of DsbBPMID: 18406599. DsbB oxidizes the periplasmic protein DsbA, which in turn oxidizes the cysteines in other periplasmic proteins to make disulfide bondsPMID: 8430071. Gene fusion approaches were used to show that (i) DsbB is a membrane protein which spans the membrane four times and (ii) both the N- and C-termini of the protein are in the cytoplasmPMID: 7957076. The structure of DsbB revealed four transmembrane helices and one short horizontal helix juxtaposed with Cys130 in the mobile periplasmic loopPMID: 17110337. Disulfide bridge (41 44;104 130) PMID: 10064586 | PMID: 15919996 PMID: 17110337 PMID: 18406599 PMID: 7957076 PMID: 8430071 | in/in | Integral Inner Membrane | B | B | IM (predicted), Detected in IM Fraction | Cell inner membrane | Integral Membrane Protein | SRP | SEC-YidC | yes | yes | N | 25 | 0.286 | TMH | 0 | 4.8983 | - | 4 | 4 | N | i12-33o45-64i71-89o145-166i | CytoplasmicMembrane | 10 | 15 | ||||
| FLGI_ECOLI | P0A6S3 | flgI fla FIX flaM | fla FIX flaM | b1080 JW1067 | Flagellar periplasmic protein (SEC through IM), P-ring FlgI | Periplasmic (Experimental) | P ring protein. Substrate of periplasmic disulfide oxidoreductases DsbA. Might be is co-translationally translocated through Sec translocon. Identified DsbA substrate PMID: 19953543 PMID: 12730325 Potential substrate for the SRP pathway. When fused to cytoplasmic protein thioredoxin promotes signal recognition particle-dependent translocation PMID: 15838024 | PMID: 12730325 PMID: 15838024 | Periplasmic | G | G | N/A | Periplasm Bacterial flagellum basal body | Periplasmic (Experimental) | flagellum | SRP (exp) | SEC | DSBA | Fla | no | yes | Y | 20 | 0.783 | SpI | 19 | 15.6262 | - | 0 | 0 | Y | n4-14c19/20o | Periplasmic | 10 | 63 | ||
| GSH1_ECOLI | P0A6W9 | gshA gsh-I | gsh-I | b2688 JW2663 | cytoplasmic protein, Glutamate--cysteine ligase GshA | Disulfide bond-containing protein PMID: 25664544 | Cytoplasmic | A | A | N/A | Cytoplasmic | yes | yes | N | 21 | 0.149 | CYT | 0 | -0.200913 | - | 0 | 0 | N | i | Cytoplasmic | 9 | 26 | ||||||||||
| HSLO_ECOLI | P0A6Y5 | hslO yrfI | yrfI | b3401 JW5692 | cytoplasmic peripheral IM protein, 33 kDa chaperonin HslO | Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) | Disulfide bridge (230 232;263 266) PMID: 10764757 | PMID: 23230279 | Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm | A, F1 | A,F1 | IM (predicted) | Cytoplasm | Cytoplasmic | yes | yes | N | 63 | 0.125 | CYT | 0 | -0.200913 | - | 0 | 0 | N | i | Cytoplasmic | 10 | 88 | |||||||
| MSRA_ECOLI | P0A744 | msrA pms | pms | b4219 JW4178 | cytoplasmic protein, Peptide methionine sulfoxide reductase MsrA | Cytoplasmic (Experimental) | YeaA has been viewed through fluorescence microscopy and located evenly throughout cytoplasm. Small scale study of labeling chromosomal coding DNA sequences (CDSs) with fluorescent protein genes. PMID: 17272300 Disulfide bridge (52 199); (199 207) | PMID: 17272300 | Cytoplasmic | A | A | N/A | Cytoplasmic | yes | yes | N | 16 | 0.11 | CYT | 0 | -0.200913 | - | 0 | 0 | N | i | Unknown | 2 | 85 | ||||||||
| MSRB_ECOLI | P0A746 | msrB yeaA | yeaA | b1778 JW1767 | cytoplasmic protein, Peptide methionine sulfoxide reductase MsrB | Cytoplasmic (Experimental) | Fluorescent fusions of YeaA have been monitored via fluorescence microscopy. YeaA was found to be located evenly throughout cytoplasm. Disulfide bond-containing protein PMID: 25664544 | PMID: 17272300 | Cytoplasmic | A | A | N/A | Cytoplasmic | yes | yes | N | 62 | 0.122 | CYT | 0 | -0.200913 | - | 0 | 0 | N | i | Cytoplasmic | 9 | 96 | ||||||||
| NAGB_ECOLI | P0A759 | nagB glmD | glmD | b0678 JW0664 | cytoplasmic peripheral IM protein, Glucosamine-6-phosphate deaminase NagB | Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) | Disulfide bridge (219 219) PMID: 8240271 | PMID: 23230279 | Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm | A, F1 | A,F1 | IM (predicted) | Cytoplasmic | yes | yes | N | 32 | 0.107 | CYT | 0 | -0.200913 | - | 0 | 0 | N | i | Cytoplasmic | 8 | 28 | ||||||||
| PYRI_ECOLI | P0A7F3 | pyrI | b4244 JW4203 | cytoplasmic protein, Aspartate carbamoyltransferase regulatory chain PyrI | Cytoplasmic (Experimental) | PMID: 3912513 PMID: 8740179 | Cytoplasmic | A | A | N/A | Cytoplasmic (Experimental) | yes | yes | N | 37 | 0.104 | CYT | 0 | -0.200913 | - | 0 | 0 | N | i | Unknown | 2 | 116 | ||||||||||
| RECA_ECOLI | P0A7G6 | recA lexB recH rnmB tif umuB zab | lexB recH rnmB tif umuB zab | b2699 JW2669 | cytoplasmic peripheral IM protein, RecA | Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable) | Protein of Escherichia coli binds specifically to acidic phospholipids such as cardiolipin and phosphatidylglycerol.The interaction of RecA protein with cardiolipin and phosphatidylglycerol, which represent two of the three major phospholipids of the E. coli membrane, may be physiologically important, as it provides a possible mechanism for the RecA-membrane association during the SOS response. Trigger factor-sensitive aggregation. Disulfide bond-containing protein PMID: 25664544 | PMID: 2228969 | Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm | A, F1 | A,F1 | Soluble IM Fraction | Cytoplasm | Cytoplasmic | TF | no | yes | N | 3 | 0.103 | CYT | 0 | -0.200913 | - | 0 | 0 | N | i | Cytoplasmic | 10 | 88 | ||||||
| RL31_ECOLI | P0A7M9 | rpmE | b3936 JW3907 | ribosomal protein, 50S L31 RpmE | Cytoplasmic, Ribosomal (Probable) | Identified in nucleoids collected by sucrose gradient centrifugation and analyzed by liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS). PMID: 21541338 Disulfide bond-containing protein PMID: 25664544 | PMID: 21541338 | Cytoplasmic, Ribosomal | A, r | A,r | N/A | Cytoplasmic | ribosome | yes | yes | N | 31 | 0.105 | CYT | 0 | -0.200913 | - | 0 | 0 | N | i | Cytoplasmic | 9 | 66 | ||||||||
| RS12_ECOLI | P0A7S3 | rpsL strA | strA | b3342 JW3304 | ribosomal protein, 30S S12 RpsL | Cytoplasmic, Ribosomal (Probable) | Identified in nucleoids collected by sucrose gradient centrifugation and analyzed by liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS). PMID: 21541338 Disulfide bond-containing protein PMID: 25664544 | PMID: 21541338 | Cytoplasmic, Ribosomal | A, r | A,r | Soluble IM Fraction | Cytoplasmic | ribosome | no | yes | N | 27 | 0.105 | CYT | 0 | -0.200913 | - | 0 | 0 | N | i | Cytoplasmic | 10 | 84 | |||||||
| TPX_ECOLI | P0A862 | tpx yzzJ | yzzJ | b1324 JW1317 | cytoplasmic peripheral IM protein, Thiol peroxidase Tpx | Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) | Binds to TrxA. although initially reported to be a periplasmic enzyme, the formation of a mixed-disulfide complex with TrxA indicates a cytoplasmic localization. PMID: 7499381 PMID: 9298646 Disulfide bond-containing protein PMID: 25664544 | PMID: 23230279 PMID: 7499381 PMID: 9298646 | Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm | A, F1 | A,F1 | N/A | Periplasm | Periplasmic (Experimental) | yes | yes | N | 33 | 0.123 | CYT | 0 | -0.200913 | - | 0 | 0 | N | i | Periplasmic | 10 | ||||||||
| FLHD_ECOLI | P0A8S9 | flhD flbB | flbB | b1892 JW1881 | nucleoid protein, transcriptional regulator FlhD | Cytoplasmic, Nucleoid (Experimental) | Disulfide bridge (65 65) | PMID: 23138451 | Cytoplasmic, Nucleoid | A, N | A,N | N/A | Cytoplasm | Cytoplasmic | no | yes | N | 22 | 0.156 | CYT | 0 | -0.200913 | - | 0 | 0 | N | i | Cytoplasmic | 9 | 88 | |||||||
| OMPA_ECOLI | P0A910 | ompA con tolG tut | con tolG tut | b0957 JW0940 | OM b-barrel protein (SEC through IM, inserted in OM by BAM), OmpA | Outer Membrane b-barrel protein (Experimental) Outer Membrane b-barrel protein (Potential) Outer Membrane b-barrel protein (Probable) | Substrate of periplasmic peptidyl prolyl cis???trans isomerase SurA. Potential pubstrate of periplasmic chaperones Skp. Trigger factor-sensitive aggregation. Trigger factor-sensitive aggregation. Copurifies with trigger factor in pull down assays. The Lipid Bilayer-Inserted Membrane Protein BamA of Escherichia coli Facilitates Insertion and Folding of Outer Membrane Protein A from Its Complex with Skp. Outer membrane protein determined by LC-MS (Gevaert et al 2002). Outer membrane protein determined by 2D gel electrophoresis (Lopez-Campistrous et al 2005, Fountoulakis et al 2003, Molloy et al 2000) Identified in nucleoids collected by sucrose gradient centrifugation and analyzed by liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS). PMID: 21541338 Potential Skp substrate PMID: 19953543 Identified SurA substrates PMID: 19953543 Identified DsbA substrate PMID: 19953543 PMID: 9298646 SecA is sufficient to transport a secretory protein with hydrophobic signal sequence across SecYEG, but efficient translocation requires SRP in Addition PMID: 24717922 Disulfide bridge (311 323) Disulfide bond-containing protein PMID: 25664544 | PMID: 10806384 PMID: 16079137 PMID: 19953543 PMID: 20932056 PMID: 21778229 PMID: 24717922 PMID: 7813480 PMID: 9298646 PMID: 9808047 | Outer Membrane b-barrel protein | H | H | Soluble IM Fraction | Cell outer membrane | Outer Membrane ??-barrel protein (Experimental) | TF | SEC | SKP,SURA,DEGP,DSBA | BAM | yes | yes | Y | 22 | 0.872 | SpI | 21 | 28.1613 | - | 0 | 0 | Y | n5-16c21/22o | OuterMembrane | 10 | 10 | |||
| ALF1_ECOLI | P0A991 | fbaB dhnA | dhnA | b2097 JW5344 | cytoplasmic peripheral IM protein, Fructose-bisphosphate aldolase class 1 FbaB | Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) | Disulfide bond-containing protein PMID: 25664544 | PMID: 23230279 | Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm | A, F1 | A,F1 | N/A | Cytoplasm | Cytoplasmic | no | yes | N | 64 | 0.115 | CYT | 0 | -0.200913 | - | 0 | 0 | N | i | Unknown | 2 | 4 | |||||||
| FUR_ECOLI | P0A9A9 | fur | b0683 JW0669 | nucleoid protein, Ferric uptake regulation Fur | Cytoplasmic, Nucleoid (Experimental) | Disulfide bond-containing protein PMID: 25664544 | PMID: 23138451 | Cytoplasmic, Nucleoid | A, N | A,N | N/A | Cytoplasm | Cytoplasmic | yes | yes | N | 37 | 0.121 | CYT | 0 | -0.200913 | - | 0 | 0 | N | i | Cytoplasmic | 10 | 109 | ||||||||
| G3P1_ECOLI | P0A9B2 | gapA | b1779 JW1768 | cytoplasmic peripheral IM protein, Glyceraldehyde-3-phosphate dehydrogenase A GapA | Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) | Trigger factor-sensitive aggregation. Disulfide bond-containing protein PMID: 25664544 | PMID: 23230279 | Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm | A, F1 | A,F1 | Soluble IM Fraction | Cytoplasm | Cytoplasmic | TF | yes | yes | N | 21 | 0.121 | CYT | 0 | -0.200913 | - | 0 | 0 | N | i | Cytoplasmic | 9 | 73 | |||||||
| ACEA_ECOLI | P0A9G6 | aceA icl | icl | b4015 JW3975 | cytoplasmic peripheral IM protein, Isocitrate lyase AceA | Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) | Disulfide bond-containing protein PMID: 25664544 | PMID: 23230279 | Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm | A, F1 | A,F1 | N/A | Cytoplasmic | yes | yes | N | 30 | 0.126 | CYT | 0 | -0.200913 | - | 0 | 0 | N | i | Cytoplasmic | 10 | 42 | ||||||||
| DLDH_ECOLI | P0A9P0 | lpdA lpd | lpd | b0116 JW0112 | cytoplasmic peripheral IM protein, Dihydroyl dehydrogenase LpdA | Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable) | Trigger factor-sensitive aggregation. PMID: 6352260 Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). PMID: 23230279 Disulfide bridge (45 50) Disulfide bond-containing protein PMID: 25664544 | PMID: 16079137 PMID: 23230279 PMID: 6352260 | Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm | A, F1 | A,F1 | Soluble IM Fraction | Cytoplasm | Cytoplasmic (Experimental) | TF | yes | yes | N | 23 | 0.176 | CYT | 0 | -0.200913 | - | 0 | 0 | N | i | Cytoplasmic | 10 | 79 | ||||||
| TRXB_ECOLI | P0A9P4 | trxB | b0888 JW0871 | cytoplasmic protein, Thioredoxin reductase TrxB | Disulfide bridge (136 139) PMID: 8114095 | Cytoplasmic | A | A | N/A | Cytoplasm | Cytoplasmic | yes | yes | N | 26 | 0.317 | SpI | 25 | 6.69011 | - | 0 | 0 | Y | n9-17c25/26o | Cytoplasmic | 10 | 104 | ||||||||||
| THIO_ECOLI | P0AA25 | trxA fipA tsnC | fipA tsnC | b3781 JW5856 | cytoplasmic peripheral IM protein, Thioredoxin 1 TrxA | Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) | Trigger factor-sensitive aggregation. Disulfide bridge (33 36) PMID: 10489448 Disulfide bond-containing protein PMID: 25664544 | PMID: 10489448 PMID: 23230279 | Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm | A, F1 | A,F1 | N/A | Cytoplasmic | TF | no | yes | N | 20 | 0.119 | CYT | 0 | -0.200913 | - | 0 | 0 | N | i | Cytoplasmic | 9 | 89 | |||||||
| YEDF_ECOLI | P0AA31 | yedF | b1930 JW1915 | cytoplasmic protein, Putative sulfur carrier YedF | Disulfide bond-containing protein PMID: 25664544 | Cytoplasmic | A | A | N/A | Cytoplasmic | no | yes | N | 24 | 0.136 | CYT | 0 | -0.200913 | - | 0 | 0 | N | i | Unknown | 2 | 90 | |||||||||||
| DSBE_ECOLI | P0AA86 | dsbE ccmG yejQ | ccmG yejQ | b2195 JW2183 | integral IM protein (SEC in IM), Thiol:disulfide interchange DsbE | Integral Inner Membrane (Experimental) | PMID: 16364305 PMID: 9537397 | in/out | Integral Inner Membrane | B | B | N/A | Cell inner membrane; Single-pass membrane protein; Periplasmic side | Periplasmic with N-terminal membrane anchor (Experimental) | SRP | SEC | yes | yes | N | 29 | 0.264 | SpI | 27 | 8.59712 | - | 1 | 0 | Y | n6-21c30/31o | CytoplasmicMembrane | 10 | 69 | |||||
| YAJD_ECOLI | P0AAQ2 | yajD | b0410 JW0400 | cytoplasmic protein, Putative HNH nuclease YajD | Disulfide bond-containing protein PMID: 25664544 | Cytoplasmic | A | A | N/A | Cytoplasmic | yes | yes | N | 38 | 0.105 | CYT | 0 | -0.200913 | - | 0 | 0 | N | i | Unknown | 2 | 108 | |||||||||||
| YBEL_ECOLI | P0AAT9 | ybeL | b0643 JW0638 | cytoplasmic protein, YbeL | Disulfide bond-containing protein PMID: 25664544 | Cytoplasmic | A | A | N/A | Cytoplasmic | yes | yes | N | 22 | 0.103 | CYT | 0 | -0.200913 | - | 0 | 0 | N | i | Cytoplasmic | 8 | 95 | |||||||||||
| YBGS_ECOLI | P0AAV6 | ybgS | b0753 JW0736 | periplasmic protein (SEC through IM), YbgS | Disulfide bond-containing protein PMID: 25664544 | Periplasmic | G | G | N/A | Periplasmic | SEC | yes | yes | Y | 25 | 0.845 | SpI | 24 | 32.7676 | - | 0 | 0 | Y | n6-17c24/25o | Periplasmic | 9 | |||||||||||
| YCAR_ECOLI | P0AAZ7 | ycaR | b0917 JW0900 | cytoplasmic protein, UPF0434 YcaR | Disulfide bond-containing protein PMID: 25664544 | Cytoplasmic | A | A | N/A | Cytoplasmic | no | yes | N | 56 | 0.117 | CYT | 0 | -0.200913 | - | 0 | 0 | N | i | Unknown | 2 | ||||||||||||
| EFEO_ECOLI | P0AB24 | efeO ycdO | ycdO | b1018 JW1003 | periplasmic protein (SEC through IM), Iron uptake system component EfeO | Periplasmic (Experimental) Periplasmic (Probable) | Identified DsbA substrate PMID: 19953543 PMID: 12107143 Potential substrate for the SRP pathway. It was found to be down-regulated in the periplasmic compartment of SRP-depleted cells. PMID: 22030350 Disulfide bond-containing protein PMID: 25664544 | PMID: 12107143 PMID: 16551627 PMID: 22030350 | Periplasmic | G | G | N/A | Periplasm | Periplasmic (Experimental) | SEC | DSBA | yes | yes | Y | 27 | 0.767 | SpI | 26 | 18.2235 | - | 0 | 0 | Y | n9-18c26/27o | Periplasmic | 10 | 42 | |||||
| YMGD_ECOLI | P0AB46 | ymgD | b1171 JW5177 | periplasmic protein (SEC through IM), YmgD | Disulfide bond-containing protein PMID: 25664544 | Periplasmic | G | G | N/A | Periplasmic | SEC | no | yes | Y | 20 | 0.656 | SpI | 19 | 14.7848 | - | 0 | 0 | Y | n4-14c19/20o | Unknown | 2 | 86 | ||||||||||
| ACYP_ECOLI | P0AB65 | yccX | b0968 JW5131 | cytoplasmic protein, Acylphosphatase YccX | Disulfide bridge (5 49) PMID: 17134700 | Cytoplasmic | A | A | N/A | Cytoplasmic | no | yes | N | 28 | 0.119 | CYT | 0 | -0.200913 | - | 0 | 0 | Y | n4-11c16/17o | Cytoplasmic | 9 | 103 | |||||||||||
| CDD_ECOLI | P0ABF6 | cdd | b2143 JW2131 | cytoplasmic protein, Cytidine deaminase Cdd | One very weak transmembrane reg ion pred ict ion. Disulfide bond-containing protein PMID: 25664544 | Cytoplasmic | A | A | IM (predicted) | Cytoplasmic | yes | yes | N | 26 | 0.113 | CYT | 0 | -0.200913 | - | 0 | 0 | N | i | Unknown | 2 | 42 | |||||||||||
| NRFA_ECOLI | P0ABK9 | nrfA | b4070 JW4031 | periplasmic protein (SEC through IM), Cytochrome c-552 NrfA | Periplasmic (Experimental) | Disulfide bond-containing protein PMID: 25664544 | PMID: 793493 | Periplasmic | G | G | N/A | Periplasm | Periplasmic (Experimental) | SEC | yes | yes | Y | 27 | 0.665 | SpI | 26 | 14.744 | - | 0 | 0 | Y | n11-21c26/27o | Periplasmic | 10 | 25 | |||||||
| NRFB_ECOLI | P0ABL1 | nrfB yjcI | yjcI | b4071 JW4032 | periplasmic protein (SEC through IM), Cytochrome c-type NrfB | Periplasmic (Experimental) | Disulfide bond-containing protein PMID: 25664544 | PMID: 15280383 | Periplasmic | G | G | N/A | Periplasm | Periplasmic (Experimental) | SEC | no | yes | Y | 33 | 0.498 | SpI | 25 | 6.62799 | - | 0 | 0 | Y | n7-18c23/24o | Periplasmic | 9 | 16 | ||||||
| NAPB_ECOLI | P0ABL3 | napB yejY | yejY | b2203 JW5367 | periplasmic protein (SEC through IM), nitrate reductase, electron transfer subunit NapB | Periplasmic (Experimental) Periplasmic (Probable) | Nitrate reductase, napFDAGHBC, is located in the periplasm. E. coli also expresses two membrane-bound proton-translocating nitrate reductases, encoded by the narGHJI and narZYWV operons. PMID: 11844760 PMID: 10234835 Disulfide bond-containing protein PMID: 25664544 | PMID: 10234835 PMID: 10548535 PMID: 11844760 PMID: 17130127 | Periplasmic | G | G | N/A | Periplasm | Periplasmic (Experimental) | SEC | yes | yes | Y | 28 | 0.825 | SpI | 27 | 14.6891 | - | 0 | 0 | Y | n9-20c27/28o | Periplasmic | 10 | |||||||
| SFMA_ECOLI | P0ABW5 | sfmA | b0530 JW0519 | Fimbrial periplasmic protein (SEC through IM), SfmA | Potential fimbrial sfm operon (class ??1); Fimbrial Subunit. Disulfide bridge (41 81) | Periplasmic | G | G | N/A | Fimbrium | Periplasmic | fimbriae | SEC | CU | yes | yes | Y | 23 | 0.832 | SpI | 22 | 21.4596 | - | 0 | 0 | Y | n6-17c22/23o | Extracellular | 10 | 83 | |||||||
| FUMA_ECOLI | P0AC33 | fumA | b1612 JW1604 | cytoplasmic peripheral IM protein, Fumarate hydratase class I, aerobic FumA | Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) | Disulfide bond-containing protein PMID: 25664544 | PMID: 23230279 | Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm | A, F1 | A,F1 | N/A | Cytoplasmic | yes | yes | N | 57 | 0.116 | CYT | 0 | -0.200913 | - | 0 | 0 | N | i | Cytoplasmic | 9 | 37 | |||||||||
| GLRX2_ECOLI | P0AC59 | grxB | b1064 JW1051 | cytoplasmic protein, Glutaredoxin 2 GrxB | Disulfide bridge (9 12) Disulfide bond-containing protein PMID: 25664544 | Cytoplasmic | A | A | N/A | Cytoplasmic | yes | yes | N | 33 | 0.104 | CYT | 0 | -0.200913 | - | 0 | 0 | N | i | Cytoplasmic | 8 | 65 | |||||||||||
| GLRX3_ECOLI | P0AC62 | grxC yibM | yibM | b3610 JW3585 | cytoplasmic peripheral IM protein, Glutaredoxin 3 GrxC | Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) | Disulfide bridge (12 15) | PMID: 23230279 | Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm | A, F1 | A,F1 | N/A | Cytoplasmic | yes | yes | N | 30 | 0.112 | CYT | 0 | -0.200913 | - | 0 | 0 | N | i | Unknown | 2 | 79 | ||||||||
| NRDH_ECOLI | P0AC65 | nrdH ygaN | ygaN | b2673 JW2648 | cytoplasmic protein, Glutaredoxin-like NrdH NrdH | Disulfide bridge (11 14) | Cytoplasmic | A | A | N/A | Cytoplasmic | no | yes | N | 57 | 0.105 | CYT | 0 | -0.200913 | - | 0 | 0 | N | i | Unknown | 2 | 102 | ||||||||||
| ISCU_ECOLI | P0ACD4 | iscU nifU yfhN | nifU yfhN | b2529 JW2513 | cytoplasmic peripheral IM protein, Iron-sulfur cluster assembly scaffold IscU | Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) | IscS is critical for iron-sulfur (Fe-S) cluster formation and repair. IscS transfers sulfur to the coregulated "scaffold" protein IscU (NifU), on which Fe-S clusters are assembled. Disulfide bond-containing protein PMID: 25664544 | PMID: 23230279 | Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm | A, F1 | A,F1 | N/A | Cytoplasmic | yes | yes | N | 20 | 0.105 | CYT | 0 | -0.200913 | - | 0 | 0 | N | i | Cytoplasmic | 9 | 97 | ||||||||
| MBHM_ECOLI | P0ACE0 | hybC | b2994 JW2962 | periplasmic peripheral IM protein (TAT via piggyback through IM), Hydrogenase-2 large chain HybC | Peripheral inner membrane protein facing the periplasm (Experimental) | The protein is attached to this location through a 5kDa segment of its small subunit hybO that is has a TAT signal peptide. Secreted using the TAT system (piggyback). PMID: 10224080 An active tryptic fragment of membrane-bound hydrogenase isoenzyme 2 from anaerobically grown Escherichia coli has been purified. This indicates that HYD2 is attached to the periplasmic side of the cytoplasmic membrane.PMID: 3516690 The intracellular availability of nickel is essential for the processing of the large subunit and for the transmembrane translocation of HYD2. PMID: 8772179 PMID: 9738917 Disulfide bond-containing protein PMID: 25664544 | PMID: 3516690 PMID: 8772179 PMID: 9738917 | Peripheral inner membrane protein facing the periplasm | F2 | F2 | Soluble IM Fraction | Cell membrane; Peripheral membrane protein | Periplasmic (Experimental) | TAT (piggyback) | yes | yes | N | 34 | 0.139 | CYT | 0 | -0.200913 | - | 0 | 0 | N | i | CytoplasmicMembrane | 10 | 28 | |||||||
| OXYR_ECOLI | P0ACQ4 | oxyR momR mor | momR mor | b3961 JW3933 | nucleoid protein, Hydrogen peroxide-inducible genes activator OxyR | Cytoplasmic, Nucleoid (Experimental) | Disulfide bridge (180 259) PMID: 12015987 Disulfide bridge (199 208) PMID: 9497290 | PMID: 23138451 | Cytoplasmic, Nucleoid | A, N | A,N | Soluble IM Fraction | Cytoplasmic | yes | yes | N | 22 | 0.192 | CYT | 0 | -0.200913 | - | 0 | 0 | N | i | Cytoplasmic | 9 | 16 | ||||||||
| YDCA_ECOLI | P0ACW4 | ydcA | b1419 JW1416 | periplasmic protein (SEC through IM), YdcA | Disulfide bond-containing protein PMID: 25664544 | Periplasmic | G | G | N/A | Periplasmic | SEC | no | yes | Y | 21 | 0.761 | SpI | 20 | 12.9463 | - | 0 | 0 | Y | n4-15c20/21o | Unknown | 2 | |||||||||||
| IVY_ECOLI | P0AD59 | ivy ykfE | ykfE | b0220 JW0210 | periplasmic protein (SEC through IM), Inhibitor of vertebrate lysozyme Ivy | Periplasmic (Experimental) | Experimentally verified gene-product. Verified that the signal peptide is cleaved. PMID: 11278658 Disulfide bridge (85 90) Disulfide bond-containing protein PMID: 25664544 | PMID: 11278658 | Periplasmic | G | G | Soluble IM Fraction | Periplasm | Periplasmic (Experimental) | SEC | yes | yes | Y | 29 | 0.849 | SpI | 28 | 16.6514 | - | 0 | 0 | Y | n13-23c28/29o | Periplasmic | 10 | 27 | ||||||
| AMPH_ECOLI | P0AD70 | ampH yaiH | yaiH | b0376 JW5052 | cytoplasmic peripheral IM protein (SEC through IM), D-alanyl-D-alanine-carboxypeptidase/endopeptidase AmpH | Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable) | Potential substrate of periplasmic peptidyl prolyl cis???trans isomerase SurA. Sequence contains known binding motif of SurA [RYEF]X[RYEF][P]. The N-terminal export signal of AmpH is processed, but the protein remains membrane associated. Disulfide bond-containing protein PMID: 25664544 | PMID: 22001512 | Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm | A, F1 | A,F1 | N/A | Cell inner membrane | Periplasmic | SURA | yes | yes | Y | 22 | 0.613 | SpI | 21 | 16.801 | - | 0 | 0 | Y | n5-16c21/22o | Periplasmic | 9 | 41 | ||||||
| LIVJ_ECOLI | P0AD96 | livJ | b3460 JW3425 | periplasmic protein (SEC through IM), Leu/Ile/Val-binding LivJ | Periplasmic (Experimental) | Substrate of periplasmic disulfide oxidoreductases DsbA. Identified DsbA substrate PMID: 19953543 PMID: 9298646 Potential substrate for the SRP pathway. It was found to be down-regulated in the periplasm and up-regulated in the cytoplasm of SRP-depleted cells. PMID: 22030350 Disulfide bridge (76 101) Disulfide bond-containing protein PMID: 25664544 | PMID: 22030350 PMID: 9298646 | Periplasmic | G | G | N/A | Periplasm | Periplasmic (Experimental) | SEC | DSBA | yes | yes | Y | 24 | 0.832 | SpI | 23 | 20.6668 | - | 0 | 0 | Y | n7-18c23/24o | Periplasmic | 10 | 96 | ||||||
| OSME_ECOLI | P0ADB1 | osmE anr | anr | b1739 JW1728 | OM lipoprotein (SEC through IM), inserted in OM by LOL), Osmotically-inducible putative OsmE | Outer Membrane Lipoprotein (Experimental) | Proven signal peptidase II substrate. Outer membrane protein determined by 2D gel electrophoresis (Lopez-Campistrous et al 2005) Identified in nucleoids collected by sucrose gradient centrifugation and analyzed by liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS). PMID: 21541338 PMID: 10806384 Disulfide bond-containing protein PMID: 25664544 | PMID: 10806384 PMID: 15174130 PMID: 15911532 PMID: 20932056 | Outer Membrane Lipoprotein | I | I | Soluble IM Fraction | Cell inner membrane | Outer membrane Lipoprotein (Experimental) | SEC | LOL | yes | yes | Y | 24 | 0.207 | SpII | 20 | 20.0226 | Pos+2=T | 1 | 0 | Y | n7-18c23/24o | Unknown | 2 | 98 | |||||
| YGGN_ECOLI | P0ADS9 | yggN | b2958 JW2925 | periplasmic protein (SEC through IM), YggN | Periplasmic (Experimental) | Substrate of periplasmic disulfide oxidoreductases DsbA. Identified DsbA substrate PMID: 19953543 PMID: 14726535 | PMID: 14726535 | Periplasmic | G | G | N/A | Periplasmic(experimental) | SEC | DSBA | yes | yes | Y | 21 | 0.797 | SpI | 20 | 21.2471 | - | 0 | 0 | Y | n6-16c20/21o | Unknown | 2 | 80 | |||||||
| YGIB_ECOLI | P0ADT2 | ygiB | b3037 JW5927 | cytoplasmic peripheral IM protein, UPF0441 YgiB | Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) | YgiB and ygiC genes are expressed in the same operon as tolC and have homologues (yjfM and yjfC) located elsewhere on the chromosome. Mutational inactivation of the outer membrane channelTolC (a key component of multidrug efflux and type I secretion transporters) renders cells highly susceptible to antibiotics and leads to defects in secretion of protein toxins and the loss of TolC impairs cell division and growth in minimal glucose medium due to metabolic shutdown. The TolC-dependent phenotype was further exacerbated by the loss of ygiB and ygiC genes. YgiBC (and YjfMC) may have parallel but independent functions from TolC. Disulfide bond-containing protein PMID: 25664544 | PMID: 20545840 PMID: 23230279 | Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm | A, F1 | A,F1 | Soluble IM Fraction | Cytoplasmic | yes | yes | N | 36 | 0.178 | SpII | 36 | 9.72422 | Pos+2=E | 0 | 0 | Y | n21-32c37/38o | Periplasmic | 9 | ||||||||||
| YHEV_ECOLI | P0ADW8 | yheV | b4551 JW3312 | cytoplasmic protein, YheV | Disulfide bond-containing protein PMID: 25664544 | Cytoplasmic | A | A | N/A | Cytoplasmic | no | yes | N | 24 | 0.14 | CYT | 0 | -0.200913 | - | 0 | 0 | N | i | Unknown | 2 | ||||||||||||
| AHPC_ECOLI | P0AE08 | ahpC | b0605 JW0598 | cytoplasmic peripheral IM protein, Alkyl hydroperoxide reductase C AhpC | Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) | Disulfide bridge (47 47;166 166) Disulfide bond-containing protein PMID: 25664544 | PMID: 23230279 | Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm | A, F1 | A,F1 | N/A | Cytoplasm | Cytoplasmic | yes | yes | N | 49 | 0.111 | CYT | 0 | -0.200913 | - | 0 | 0 | N | i | Cytoplasmic | 10 | 90 | ||||||||
| BCP_ECOLI | P0AE52 | bcp | b2480 JW2465 | cytoplasmic protein, Peroxiredoxin Bcp | Disulfide bond-containing protein PMID: 25664544 | Cytoplasmic | A | A | N/A | Cytoplasmic | yes | yes | N | 51 | 0.123 | CYT | 0 | -0.200913 | - | 0 | 0 | N | i | Cytoplasmic | 8 | ||||||||||||
| CREA_ECOLI | P0AE91 | creA yjjD | yjjD | b4397 JW4360 | periplasmic protein (SEC through IM), CreA | Identified DsbA substrate PMID: 19953543 Disulfide bond-containing protein PMID: 25664544 | Periplasmic | G | G | N/A | Periplasmic | SEC | DSBA | yes | yes | Y | 22 | 0.8 | SpI | 21 | 14.9398 | - | 0 | 0 | Y | n6-16c21/22o | Unknown | 2 | |||||||||
| DSBA_ECOLI | P0AEG4 | dsbA dsf ppfA | dsf ppfA | b3860 JW3832 | periplasmic protein (SEC through IM), Thiol:disulfide interchange DsbA | Periplasmic (Experimental) | Periplasmic disulfide isomerase DsbC cooperates with SurA and the thiol oxidase DsbA in the folding of the essential ??-barrel protein LptD. DsbA is co-translationally translocated through Sec translocon. PMID: 9298646 DsbA, is a substrate for the SRP pathway. PMID: 15838024 DsbA, is a substrate for the SRP pathway. PMID: 13129941 Disulfide bridge (49 52) PMID: 9300489 Disulfide bond-containing protein PMID: 25664544 | PMID: 13129941 PMID: 15838024 PMID: 9298646 | Periplasmic | G | G | N/A | Periplasm | Periplasmic (Experimental) | SRP (exp) | SEC | yes | yes | Y | 20 | 0.75 | SpI | 19 | 16.7987 | - | 0 | 0 | Y | n4-15c19/20o | Periplasmic | 10 | 71 | |||||
| DSBC_ECOLI | P0AEG6 | dsbC xprA | xprA | b2893 JW2861 | periplasmic protein (SEC through IM), Thiol:disulfide interchange DsbC | Periplasmic (Experimental) Periplasmic (Probable) | Periplasmic disulfide isomerase DsbC cooperates with SurA and the thiol oxidase DsbA in the folding of the essential ??-barrel protein LptD. PMID: 9298646 Disulfide bridge (118 121;161 183) PMID: 10700276 Disulfide bond-containing protein PMID: 25664544 | PMID: 8168498 PMID: 9298646 | Periplasmic | G | G | N/A | Periplasm | Periplasmic (Experimental) | SEC | yes | yes | Y | 21 | 0.778 | SpI | 20 | 21.5074 | - | 0 | 0 | Y | n4-15c20/21o | Periplasmic | 10 | 82 | ||||||
| HDEA_ECOLI | P0AES9 | hdeA yhhC yhiB | yhhC yhiB | b3510 JW3478 | periplasmic protein (SEC through IM), Acid stress chaperone HdeA | Periplasmic (Experimental) Periplasmic (Probable) | Experimentally verified gene-product. Verified that the signal peptide is cleaved. Falls into an unstructured form that is essential for its chaperone activity. The protein is dimeric at neutral pH but dissociates into a partially unfolded monomer at pH 2.0 PMID: 20080625. PMID: 8455549 Potential substrate of periplasmic chaperone YfgM. PMID: 25403562 Putative substrate for the periplasmic chaperone YfgM. PMID: 25403562 Disulfide bridge (39 87) Disulfide bond-containing protein PMID: 25664544 | PMID: 10623550 PMID: 17085547 PMID: 25403562 PMID: 8455549 | Periplasmic | G | G | N/A | Periplasm | Periplasmic (Experimental) | SEC | YfgM | yes | yes | Y | 22 | 0.698 | SpI | 21 | 16.385 | - | 1 | 0 | Y | n4-15c21/22o | Unknown | 2 | 108 | |||||
| HDEB_ECOLI | P0AET2 | hdeB yhhD yhiC | yhhD yhiC | b3509 JW5669 | periplasmic protein (SEC through IM), Acid stress chaperone HdeB | Periplasmic (Experimental) Periplasmic (Probable) | Experimentally verified gene-product. Verified that the signal peptide is cleaved. PMID: 8455550 Potential substrate of periplasmic chaperone YfgM. PMID: 25403562 Putative substrate for the periplasmic chaperone YfgM. PMID: 25403562 Disulfide bond-containing protein PMID: 25664544 | PMID: 17085547 PMID: 25403562 PMID: 8455550 | Periplasmic | G | G | N/A | Periplasm | Periplasmic (Experimental) | SEC | YfgM | yes | yes | Y | 30 | 0.837 | SpI | 29 | 16.8932 | - | 0 | 0 | Y | n10-21c29/30o | Unknown | 2 | 63 | |||||
| HDHA_ECOLI | P0AET8 | hdhA hsdH | hsdH | b1619 JW1611 | cytoplasmic protein, 7-alpha-hydroxysteroid dehydrogenase HdhA | Disulfide bond-containing protein PMID: 25664544 | Cytoplasmic | A | A | N/A | Cytoplasmic | yes | yes | N | 48 | 0.2 | CYT | 0 | -0.200913 | - | 0 | 0 | N | i | Cytoplasmic | 9 | 68 | ||||||||||
| HISJ_ECOLI | P0AEU0 | hisJ | b2309 JW2306 | periplasmic protein (SEC through IM), Histidine-binding periplasmic HisJ | Periplasmic (Experimental) | Substrate of periplasmic disulfide oxidoreductases DsbA. Identified DsbA substrate PMID: 19953543 PMID: 9298646 Disulfide bridge (60 67) Disulfide bond-containing protein PMID: 25664544 | PMID: 9298646 | Periplasmic | G | G | N/A | Periplasm | Periplasmic (Experimental) | SEC | DSBA | yes | yes | Y | 23 | 0.792 | SpI | 22 | 20.7158 | - | 1 | 0 | Y | n4-14c22/23o | Periplasmic | 10 | |||||||
| YJCO_ECOLI | P0AF56 | yjcO | b4078 JW4039 | periplasmic protein (SEC through IM), Sel1-repeat-containing YjcO | Disulfide bond-containing protein PMID: 25664544 | Periplasmic | G | G | N/A | Periplasmic | SEC | yes | yes | Y | 18 | 0.752 | SpI | 17 | 13.9637 | - | 0 | 0 | Y | n4-13c17/18o | Unknown | 2 | 82 | ||||||||||
| PROX_ECOLI | P0AFM2 | proX proU | proU | b2679 JW2654 | periplasmic protein (SEC through IM), Glycine betaine/proline betaine-binding periplasmic ProX | Periplasmic (Experimental) | Potential substrate of periplasmic peptidyl prolyl cis???trans isomerase SurA; sequence contains known binding motif of SurA [RYEF]X[RYEF][P]. Identified DsbA substrate PMID: 19953543 PMID: 3305496 Disulfide bridge (157 163) Disulfide bond-containing protein PMID: 25664544 | PMID: 19953543 PMID: 3305496 | Periplasmic | G | G | N/A | Periplasm | Periplasmic (Experimental) | SEC | SURA,DSBA | yes | yes | Y | 22 | 0.863 | SpI | 21 | 16.9579 | - | 0 | 0 | Y | n5-16c21/22o | Periplasmic | 10 | 52 | |||||
| PSIF_ECOLI | P0AFM4 | psiF | b0384 JW5054 | periplasmic protein (SEC through IM), Phosphate starvation-inducible PsiF | Disulfide bond-containing protein PMID: 25664544 | Periplasmic | G | G | N/A | Periplasmic | SEC | yes | yes | Y | 22 | 0.376 | SpI | 21 | 13.709 | - | 1 | 0 | Y | n5-16c21/22o | Unknown | 2 | 44 | ||||||||||
| UGPB_ECOLI | P0AG80 | ugpB | b3453 JW3418 | periplasmic protein (SEC through IM), sn-glycerol-3-phosphate-binding periplasmic UgpB | Periplasmic (Experimental) | Identified DsbA substrate PMID: 19953543 PMID: 3062310 Disulfide bond-containing protein PMID: 25664544 | PMID: 3062310 | Periplasmic | G | G | N/A | Periplasm | Periplasmic (Experimental) | SEC | DSBA | yes | yes | Y | 24 | 0.804 | SpI | 23 | 17.1867 | - | 0 | 0 | Y | n7-18c23/24o | Periplasmic | 10 | 64 | ||||||
| SLT_ECOLI | P0AGC3 | slt sltY | sltY | b4392 JW4355 | periplasmic protein (SEC through IM), Soluble lytic murein transglycosylase Slt | Periplasmic (Experimental) | Disulfide bridge (133 166) Disulfide bond-containing protein PMID: 25664544 | PMID: 1938883 | Periplasmic | G | G | N/A | Periplasm | Periplasmic (Experimental) | SEC | yes | yes | Y | 28 | 0.803 | SpI | 27 | 12.0915 | - | 0 | 0 | Y | n11-22c27/28o | Periplasmic | 10 | 10 | ||||||
| SODC_ECOLI | P0AGD1 | sodC | b1646 JW1638 | periplasmic protein (SEC through IM), Superoxide dismutase SodC | Periplasmic (Experimental) | Disulfide bridge (74 169) PMID: 9405149 Disulfide bond-containing protein PMID: 25664544 | PMID: 8626323 | Periplasmic | G | G | N/A | Periplasm | Periplasmic (Experimental) | SEC | yes | yes | Y | 20 | 0.82 | SpI | 19 | 16.2903 | - | 0 | 0 | Y | n4-14c19/20o | Periplasmic | 10 | ||||||||
| THIO2_ECOLI | P0AGG4 | trxC yfiG | yfiG | b2582 JW2566 | cytoplasmic protein, Thioredoxin 2 TrxC | Disulfide bridge (64 67) Disulfide bond-containing protein PMID: 25664544 | Cytoplasmic | A | A | N/A | Cytoplasm | Cytoplasmic | yes | yes | N | 70 | 0.102 | CYT | 0 | -0.200913 | - | 0 | 0 | N | i | Cytoplasmic | 10 | 92 | |||||||||
| MEPA_ECOLI | P0C0T5 | mepA | b2328 JW2325 | periplasmic protein (SEC through IM), Penicillin-insensitive murein endopeptidase MepA | Periplasmic (Experimental) | Substrate of periplasmic disulfide oxidoreductases DsbC and DsbA. Identified DsbC substrate PMID: 19953543 Identified DsbA substrate PMID: 19953543 PMID: 2187143 Disulfide bridge (44 265;187 235;216 223) Disulfide bond-containing protein PMID: 25664544 | PMID: 2187143 | Periplasmic | G | G | N/A | Periplasm | Periplasmic (Experimental) | SEC | DSBA,DSBC | yes | yes | Y | 20 | 0.847 | SpI | 19 | 22.606 | - | 0 | 0 | Y | n4-14c19/20o | Periplasmic | 10 | 16 | ||||||
| DEGP_ECOLI | P0C0V0 | degP htrA ptd | htrA ptd | b0161 JW0157 | periplasmic protein (SEC through IM), serine endoprotease DegP | Periplasmic (Experimental) Periplasmic (Probable) | SecB substrate. Substrate of periplasmic disulfide oxidoreductases DsbA. HtrA was not detected by the immunoblotting technique in the periplasm or in the fraction of soluble proteins but was found in the inner membrane. DegP oligomerizes as a hexameric cage but in its presence DegP reorganizes into 12 and 24-mer cages with large chambers that house the substrate for degradation or refolding. Size exclusion chromatography and electron microscopy show that the size of the substrate molecule is the main factor conditioning the oligomeric state adopted by the enzyme. Identified DsbA substrate PMID: 19953543 PMID: 2180903 Disulfide bridges between 83 and 95 PMID: 12878036 Disulfide bridge (83 95) PMID: 12878036 Disulfide bond-containing protein PMID: 25664544 | PMID: 12878036 PMID: 2180903 PMID: 9083020 | Periplasmic | G | G | N/A | Cell inner membrane | Periplasmic (Experimental) | SECB | SEC | DSBA | yes | yes | Y | 27 | 0.841 | SpI | 26 | 28.3128 | - | 0 | 0 | Y | n4-19c26/27o | Periplasmic | 10 | 31 | ||||
| GATZ_ECOLI | P0C8J8 | gatZ | b2095 JW2082 | cytoplasmic peripheral IM protein, D-tagatose-1,6-bisphosphate aldolase subunit GatZ | Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) | Encoded in the phosphotransferase transport system the hexitol galactitol (Gat), binds to the gatY protein that encodes for a D-tagatose-1,6-bisphosphate aldolase. Trigger factor-sensitive aggregation. Trigger factor-sensitive aggregation. Copurifies with trigger factor in pull down assays. Disulfide bond-containing protein PMID: 25664544 | PMID: 23230279 | Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm | A, F1 | A,F1 | Soluble IM Fraction | Cytoplasmic | TF | yes | yes | N | 33 | 0.318 | CYT | 0 | -0.200913 | - | 0 | 0 | N | i | Cytoplasmic | 8 | 6 | ||||||||
| EFTU1_ECOLI | P0CE47 | tufA | b3339 JW3301 | cytoplasmic peripheral IM protein, Elongation factor Tu 1 TufA | Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (By similarity) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable) | Translation initiation factor EF-Tu. Ribosome association and peripheral inner membrane protein. In B. subtilis EF-Tu localizes in a helical pattern underneath the cell membrane and colocalizes with MreB, an actin-like cytoskeletal. PMID: 7525272 PMID: 7021545 Interacts with DmsD is a redox enzyme maturation protein (REMP). PMID: 20153451 Disulfide bond-containing protein PMID: 25664544 | PMID: 20133608 PMID: 20153451 PMID: 2022614 PMID: 7021545 PMID: 7525272 PMID: 775340 | Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm | A, F1 | A,F1 | N/A | Cytoplasm Cell inner membrane; Peripheral membrane protein | Cytoplasmic (Experimental) | yes | yes | N | 47 | 0.197 | CYT | 0 | -0.200913 | - | 0 | 0 | N | i | Cytoplasmic | 10 | 99 | ||||||||
| NADA_ECOLI | P11458 | nadA nicA | nicA | b0750 JW0733 | cytoplasmic protein, Quinolinate synthase A NadA | Disulfide bridge (291 294) | Cytoplasmic | A | A | N/A | Cytoplasm | Cytoplasmic | yes | yes | N | 58 | 0.105 | CYT | 0 | -0.200913 | - | 0 | 0 | N | i | Cytoplasmic | 10 | 34 | |||||||||
| TREA_ECOLI | P13482 | treA osmA | osmA | b1197 JW1186 | periplasmic protein (SEC through IM), trehalase TreA | Periplasmic (Experimental) | Potential Skp substrate PMID: 19953543 PMID: 2671658 Disulfide bond-containing protein PMID: 25664544 Disulfide bond-containing protein PMID: 25664544 | PMID: 2671658 | Periplasmic | G | G | N/A | Periplasm | Periplasmic (Experimental) | SEC | SKP | yes | yes | Y | 31 | 0.702 | SpI | 30 | 17.5054 | - | 0 | 0 | Y | n13-26c30/31o | Periplasmic | 10 | 70 | |||||
| CHIA_ECOLI | P13656 | chiA yheB | yheB | b3338 JW3300 | periplasmic & extra-cellular protein (SEC through IM), TSS secreted over OM), Probable bifunctional chitinase/lysozyme ChiA | Periplasmic, Extra-cellular (Probable) | Can be fully secreted when the cryptic T2S system is expressed. PMID: 10760150 Disulfide bridge (628 673) Disulfide bond-containing protein PMID: 25664544 | PMID: 10760150 PMID: 11118204 | Periplasmic, Extra-cellular | G, X | G,X | N/A | Periplasm | Periplasmic (Experimental) | SEC | T2S | no | yes | Y | 25 | 0.672 | SpI | 24 | 20.7505 | - | 0 | 0 | Y | n11-20c24/25o | Periplasmic | 10 | ||||||
| FUMB_ECOLI | P14407 | fumB | b4122 JW4083 | cytoplasmic protein, Fumarate hydratase class I, anaerobic FumB | Disulfide bond-containing protein PMID: 25664544 | Cytoplasmic | A | A | N/A | Cytoplasmic | yes | yes | N | 32 | 0.117 | CYT | 0 | -0.200913 | - | 0 | 0 | N | i | Cytoplasmic | 9 | 69 | |||||||||||
| SELD_ECOLI | P16456 | selD fdhB | fdhB | b1764 JW1753 | cytoplasmic protein, Selenide, water dikinase SelD | Disulfide bond-containing protein PMID: 25664544 | Cytoplasmic | A | A | N/A | Cytoplasmic | yes | yes | N | 38 | 0.121 | CYT | 0 | -0.200913 | - | 0 | 0 | N | i | Cytoplasmic | 8 | 61 | ||||||||||
| CIRA_ECOLI | P17315 | cirA cir feuA | cir feuA | b2155 JW2142 | OM b-barrel protein (SEC through IM, inserted in OM by BAM), Colicin I receptor CirA | Outer Membrane b-barrel protein (Experimental) Outer Membrane b-barrel protein (Potential) | Outer membrane protein determined by 2D gel electrophoresis (Lopez-Campistrous et al 2005, Fountoulakis et al 2003, Molloy et al 2000) Potential Skp substrate PMID: 19953543 Identified DsbA substrate PMID: 19953543 PMID: 10430870 Disulfide bond-containing protein PMID: 25664544 | PMID: 10430870 PMID: 10806384 PMID: 15911532 PMID: 19953543 PMID: 20932056 | Outer Membrane b-barrel protein | H | H | N/A | Cell outer membrane | Outer Membrane ??-barrel protein (Experimental) | SEC | DSBA,SKP | BAM | yes | yes | Y | 26 | 0.695 | SpI | 25 | 11.8271 | - | 0 | 0 | Y | n10-20c25/26o | OuterMembrane | 10 | 46 | ||||
| YJJA_ECOLI | P18390 | yjjA | b4360 JW5795 | periplasmic protein (SEC through IM), YjjA | Disulfide bond-containing protein PMID: 25664544 | Periplasmic | G | G | N/A | Periplasmic | SEC | yes | yes | Y | 26 | 0.86 | SpI | 25 | 23.6068 | - | 0 | 0 | Y | n8-19c25/26o | Unknown | 2 | 100 | ||||||||||
| AGP_ECOLI | P19926 | agp | b1002 JW0987 | periplasmic protein (SEC through IM), Glucose-1-phosphatase Agp | Periplasmic (Experimental) | Disulfide bond-containing protein PMID: 25664544 | PMID: 2153660 PMID: 9298646 | Periplasmic | G | G | N/A | Periplasm | Periplasmic (Experimental) | SEC | no | yes | Y | 23 | 0.88 | SpI | 22 | 21.9324 | - | 0 | 0 | Y | n5-16c22/23o | Periplasmic | 10 | 62 | |||||||
| TOLA_ECOLI | P19934 | tolA cim excC lky | cim excC lky | b0739 JW0729 | integral IM protein (SEC in IM), Tol-Pal system TolA | Integral Inner Membrane (Experimental) | The N-terminal coiled coil of YbgFvforms a stable trimer and controls the oligomeric status of YbgF. The stable YbgF trimer undergoes a marked change in oligomeric state on binding TolA, forming a one-to-one complex with the Tol protein. PMID: 7744737 Disulfide bridge (363 388) Disulfide bond-containing protein PMID: 25664544 | PMID: 15919657 PMID: 7744737 | in/out | Integral Inner Membrane | B | B | IM (predicted) | Cell inner membrane | Membrane anchored (Experimental) | SRP | SEC | yes | yes | N | 43 | 0.131 | CYT | 0 | -0.200913 | - | 1 | 1 | N | i12-31o | Unknown | 2 | 50 | ||||
| RNI_ECOLI | P21338 | rna rnsA | rnsA | b0611 JW0603 | periplasmic protein (SEC through IM), Ribonuclease I Rna | Periplasmic (Experimental) | Substrate of periplasmic disulfide oxidoreductases DsbC and DsbA. Identified DsbA substrate PMID: 19953543 PMID: 2253883 Disulfide bridge (103 159) | PMID: 2253883 | Periplasmic | G | G | N/A | Periplasm Cytoplasm | Periplasmic (Experimental) | SEC | DSBA,DSBC | yes | yes | Y | 24 | 0.538 | SpI | 25 | 15.6073 | - | 0 | 0 | Y | n8-18c23/24o | Periplasmic | 10 | 31 | |||||
| ECOT_ECOLI | P23827 | eco eti | eti | b2209 JW2197 | periplasmic protein (SEC through IM), Eco | Periplasmic (Experimental) Periplasmic (Probable) | Identified in periplasm by biochemical fractionation followed by two-dimensional gel electrophoresis and tandem mass spectrometry. PMID: 15911532 PMID: 9298646 Disulfide bridge (70 107) Disulfide bond-containing protein PMID: 25664544 | PMID: 15911532 PMID: 9298646 | Periplasmic | G | G | N/A | Periplasm | Periplasmic (Experimental) | SEC | no | yes | Y | 21 | 0.889 | SpI | 20 | 16.5012 | - | 0 | 0 | Y | n4-15c20/21o | Periplasmic | 10 | 85 | ||||||
| OPPA_ECOLI | P23843 | oppA | b1243 JW1235 | periplasmic protein (SEC through IM), oligopeptide-binding protein OppA | Periplasmic (Experimental) | SecB substrate. Potential Skp substrate PMID: 19953543 Identified DsbA substrate PMID: 19953543 PMID: 9298646 Potential substrate for the SRP pathway. It was found to be down-regulated in the periplasm and up-regulated in the cytoplasm of SRP-depleted cells. PMID: 22030350 Disulfide bridge (297 443) Disulfide bond-containing protein PMID: 25664544 | PMID: 22030350 PMID: 9298646 | Periplasmic | G | G | Soluble IM Fraction | Periplasm | Periplasmic (Experimental) | SECB | SEC | yes | yes | Y | 27 | 0.825 | SpI | 26 | 18.3169 | - | 1 | 0 | Y | n8-18c26/27o | Periplasmic | 10 | 73 | ||||||
| DPPA_ECOLI | P23847 | dppA | b3544 JW3513 | periplasmic protein (SEC through IM), dipeptide transport DppA | Periplasmic (Experimental) Periplasmic (Potential) Periplasmic (Probable) | Substrate of periplasmic disulfide oxidoreductases DsbA. Identified in periplasm by biochemical fractionation followed by two-dimensional gel electrophoresis and tandem mass spectrometry. PMID: 15911532 Potential Skp substrate PMID: 19953543 Identified DsbA substrate PMID: 19953543 PMID: 9298646 Disulfide bridge (34 262;450 463) Disulfide bond-containing protein PMID: 25664544 | PMID: 15911532 PMID: 19953543 PMID: 9298646 | Periplasmic | G | G | N/A | Periplasm | Periplasmic (Experimental) | SEC | DSBA,SKP | yes | yes | Y | 29 | 0.638 | SpI | 28 | 16.4393 | - | 0 | 0 | Y | n13-23c28/29o | Periplasmic | 10 | 85 | ||||||
| PSPE_ECOLI | P23857 | pspE | b1308 JW1301 | periplasmic protein (SEC through IM), Thiosulfate sulfurtransferase PspE | Periplasmic (Experimental) Periplasmic (Potential) | Experimentally verified gene-product. Verified that the signal peptide is cleaved. PMID: 9868784 Disulfide bond-containing protein PMID: 25664544 | PMID: 1712397 PMID: 9868784 | Periplasmic | G | G | N/A | Periplasm | Periplasmic (Experimental) | SEC | Prophage Transposase | yes | no | Y | 20 | 0.9 | SpI | 19 | 20.0627 | - | 0 | 0 | Y | n5-15c19/20o | Unknown | 2 | |||||||
| AMY1_ECOLI | P25718 | malS | b3571 JW3543 | periplasmic protein (SEC through IM), alpha-amylase MalS | Periplasmic (Experimental) | Disulfide bridge (57 75;121 537) PMID: 9268356 Disulfide bond-containing protein PMID: 25664544 | PMID: 1544897 | Periplasmic | G | G | N/A | Periplasm | Periplasmic (Experimental) | SEC | no | yes | Y | 18 | 0.764 | SpI | 17 | 14.5348 | - | 0 | 0 | Y | n3-11c17/18o | Periplasmic | 10 | 25 | |||||||
| END1_ECOLI | P25736 | endA | b2945 JW2912 | periplasmic protein (SEC through IM), Endonuclease-1 EndA | Periplasmic (Experimental) Periplasmic (Probable) | Identified DsbA substrate PMID: 19953543 PMID: 7867949 Disulfide bond-containing protein PMID: 25664544 | PMID: 4955462 PMID: 7867949 | Periplasmic | G | G | N/A | Periplasm | Periplasmic (Experimental) | SEC | DSBA | no | yes | Y | 23 | 0.883 | SpI | 22 | 22.5344 | - | 0 | 0 | Y | n5-16c22/23o | Unknown | 5 | 99 | ||||||
| MNMA_ECOLI | P25745 | mnmA asuE trmU ycfB | asuE trmU ycfB | b1133 JW1119 | cytoplasmic protein, tRNA-specific 2-thiouridylase MnmA | Disulfide bridge (102 199) | Cytoplasmic | A | A | N/A | Cytoplasm | Cytoplasmic | yes | yes | N | 23 | 0.288 | CYT | 0 | -0.200913 | - | 0 | 0 | N | i | Cytoplasmic | 10 | 68 | |||||||||
| LOIP_ECOLI | P25894 | loiP yggG | yggG | b2936 JW2903 | OM lipoprotein (SEC through IM), inserted in OM by LOL), Metalloprotease LoiP | Outer Membrane Lipoprotein (Experimental) Outer Membrane Lipoprotein (Probable) | Disulfide bridge (53 108) PMID: 22491786 | PMID: 17909889 PMID: 20932056 PMID: 22491786 | Outer Membrane Lipoprotein | I | I | N/A | Cell outer membrane | Outer membrane Lipoprotein | SEC | LOL | yes | yes | N | 35 | 0.146 | SpII | 18 | 25.8287 | Pos+2=Q | 0 | 0 | Y | n5-16c34/35o | Unknown | 2 | 60 | |||||
| MLIC_ECOLI | P28224 | mliC ydhA | ydhA | b1639 JW1631 | OM lipoprotein (SEC through IM), inserted in OM by LOL), Membrane-bound lysozyme inhibitor of C-type lysozyme MliC | Outer Membrane Lipoprotein (Probable) | Disulfide bridge (37 102) PMID: 16937244 | PMID: 18369469 | Outer Membrane Lipoprotein | I | I | N/A | Cell outer membrane | Outer membrane Lipoprotein | SEC | LOL | yes | yes | Y | 23 | 0.288 | SpII | 17 | 7.61087 | Pos+2=S | 0 | 0 | Y | n6-15c20/21o | Unknown | 2 | 53 | |||||
| FIMD_ECOLI | P30130 | fimD | b4317 JW5780 | Fimbrial OM b-barrel protein (SEC through IM, chaperone usher export system in OM), FimD | Outer Membrane b-barrel protein (Experimental) Outer Membrane b-barrel protein (Probable) | Fim operon (class ??1); Usher. ??uter membrane protein related to resistance to ampicillin and tetracycline. Outer membrane protein determined by LC-MS (Gevaert et al 2002) Outer membrane protein determined by 2D gel electrophoresis (Lopez-Campistrous et al 2005, Fountoulakis et al 2003) Identified in the outer membrane by biochemical fractionation followed by two-dimensional gel electrophoresis and tandem mass spectrometry. PMID: 15911532 PMID: 12842468 Disulfide bridge (855 877) | PMID: 12488465 PMID: 12842468 PMID: 15911532 | Outer Membrane b-barrel protein | H | H | N/A | Cell outer membrane | Outer Membrane ??-barrel protein (Experimental) | fimbriae | SEC | CU | yes | yes | Y | 38 | 0.415 | SpI | 37 | 1.20154 | - | 1 | 0 | Y | n23-38c45/46o | OuterMembrane | 10 | ||||||
| THIC_ECOLI | P30136 | thiC | b3994 JW3958 | cytoplasmic protein, Phosphomethylpyrimidine synthase ThiC | No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Disulfide bond-containing protein PMID: 25664544 | Cytoplasmic | A | A | IM (predicted-detected) | Cytoplasmic | yes | yes | N | 29 | 0.108 | CYT | 0 | -0.200913 | - | 0 | 0 | N | i | Cytoplasmic | 9 | 10 | |||||||||||
| ARTI_ECOLI | P30859 | artI | b0863 JW0847 | periplasmic protein (SEC through IM), Putative ABC transporter arginine-binding 2 ArtI | Periplasmic (Experimental) Periplasmic (Probable) | Identified in periplasm by biochemical fractionation followed by two-dimensional gel electrophoresis and tandem mass spectrometry. PMID: 15911532 Identified in periplasm by biochemical fractionation followed by two-dimensional gel electrophoresis and tandem mass spectrometry. PMID: 15911532 PMID: 8801422 PMID: 9298646 Potential substrate for the SRP pathway. It was found to be down-regulated in the periplasm and up-regulated in the cytoplasm of SRP-depleted cells. PMID: 22030350 Disulfide bond-containing protein PMID: 25664544 | PMID: 15911532 PMID: 22030350 PMID: 8801422 PMID: 9298646 | Periplasmic | G | G | N/A | Periplasm | Periplasmic (Experimental) | SEC | yes | yes | Y | 20 | 0.883 | SpI | 19 | 18.5986 | - | 0 | 0 | Y | n4-15c19/20o | Periplasmic | 10 | 76 | |||||||
| ARTJ_ECOLI | P30860 | artJ | b0860 JW0844 | periplasmic protein (SEC through IM), ABC transporter arginine-binding 1 ArtJ | Periplasmic (Experimental) Periplasmic (Probable) | Identified DsbA substrate PMID: 19953543 PMID: 8801423 Potential substrate for the SRP pathway. It was found to be down-regulated in the periplasmic compartment of SRP-depleted cells. PMID: 22030350 | PMID: 22030350 PMID: 8801422 PMID: 8801423 | Periplasmic | G | G | N/A | Periplasm | Periplasmic (Experimental) | SEC | DSBA | yes | yes | Y | 20 | 0.849 | SpI | 19 | 18.9337 | - | 0 | 0 | Y | n4-15c19/20o | Periplasmic | 10 | 73 | ||||||
| YEDD_ECOLI | P31063 | yedD | b1928 JW1913 | OM lipoprotein (SEC through IM, inserted in OM by LOL), YedD | Outer Membrane Lipoprotein (Experimental) | Proven pignal peptidase II substrate. Outer membrane protein determined by LC-MS (Gevaert et al 2002). Outer membrane protein determined by 2D gel electrophoresis (Lopez-Campistrous et al 2005) Identified DsbA substrate PMID: 19953543 PMID: 15174130 Disulfide bond-containing protein PMID: 25664544 | PMID: 15174130 PMID: 15911532 | Outer Membrane Lipoprotein | I | I | Soluble IM Fraction | Cell membrane | Outer membrane Lipoprotein (Experimental) | SEC | DSBA | LOL | no | yes | Y | 20 | 0.338 | SpII | 15 | 12.6143 | Pos+2=A | 0 | 0 | Y | n4-12c16/17o | Unknown | 2 | 130 | |||||
| DGCZ_ECOLI | P31129 | dgcZ ydeG ydeH | ydeG ydeH | b1535 JW1528 | cytoplasmic protein, Diguanylate cyclase DgcZ | Disulfide bond-containing protein PMID: 25664544 | Cytoplasmic | A | A | N/A | Cytoplasmic | yes | yes | N | 24 | 0.128 | CYT | 0 | -0.200913 | - | 0 | 0 | N | i | Cytoplasmic | 9 | 36 | ||||||||||
| POTF_ECOLI | P31133 | potF | b0854 JW0838 | periplasmic protein (SEC through IM), Putrescine-binding periplasmic protein PotF | Periplasmic (Experimental) | Disulfide bridge (175 239) PMID: 9651355 Disulfide bond-containing protein PMID: 25664544 | PMID: 9298646 | Periplasmic | G | G | N/A | Periplasm | Periplasmic (Experimental) | SEC | yes | yes | Y | 27 | 0.731 | SpI | 26 | 15.245 | - | 0 | 0 | Y | n9-20c26/27o | Periplasmic | 10 | 61 | |||||||
| THIB_ECOLI | P31550 | thiB tbpA yabL | tbpA yabL | b0068 JW0067 | periplasmic protein (SEC through IM), Thiamine-binding periplasmic protein ThiB | Potential substrate of periplasmic peptidyl prolyl cis???trans isomerase SurA; sequence contains known binding motif of SurA [RYEF]X[RYEF][P]. Disulfide bond-containing protein PMID: 25664544 | Periplasmic | G | G | N/A | Periplasm | Periplasmic | SEC | SURA | no | yes | Y | 19 | 0.685 | SpI | 18 | 10.962 | - | 0 | 0 | Y | n5-13c18/19o | Periplasmic | 10 | 22 | |||||||
| LPTD_ECOLI | P31554 | lptD imp ostA yabG | imp ostA yabG | b0054 JW0053 | OM b-barrel protein (SEC through IM, inserted in OM by BAM), LPS-assembly LptD | Outer Membrane b-barrel protein (Experimental) Outer Membrane b-barrel protein (Probable) | The Protein-disulfide Isomerase DsbC Cooperates with SurA and DsbA in the Assembly of LptD. Outer membrane protein determined by 2D gel electrophoresis (Lopez-Campistrous et al 2005) Potential Skp substrate PMID: 19953543 Identified SurA substrates PMID: 19953543 Identified DsbA substrate PMID: 19953543 PMID: 9298646 Disulfide bridge (31 724;173 725) | PMID: 12724388 PMID: 15911532 PMID: 16079137 PMID: 20932056 PMID: 9298646 | Outer Membrane b-barrel protein | H | H | Soluble IM Fraction | Cell outer membrane | Outer Membrane ??-barrel protein (Experimental) | SEC | DSBA,DSBC,SURA,SKP | BAM | yes | yes | Y | 25 | 0.626 | SpI | 24 | 18.6702 | - | 0 | 0 | Y | n7-17c24/25o | OuterMembrane | 10 | |||||
| RHAB_ECOLI | P32171 | rhaB | b3904 JW3875 | cytoplasmic protein, L-Rhamnulokinase RhaB | Disulfide bridge (68 222;353 370;413 417) | Cytoplasmic | A | A | N/A | Cytoplasmic | yes | yes | N | 21 | 0.191 | CYT | 0 | -0.200913 | - | 0 | 0 | N | i | Cytoplasmic | 8 | 3 | |||||||||||
| HTRE_ECOLI | P33129 | htrE | b0139 JW0135 | Fimbrial OM b-barrel protein (SEC through IM, inserted in OM by BAM), HtrE | Putative chaperone???usher fimbrial operon (class ??4); Usher. Disulfide bridge (838 862) | Outer Membrane b-barrel protein | H | H | N/A | Cell outer membrane | Outer Membrane ??-barrel protein | fimbriae | SEC | BAM | no | yes | Y | 30 | 0.677 | SpI | 29 | 6.72527 | - | 0 | 0 | N | i | OuterMembrane | 10 | 8 | |||||||
| YEBF_ECOLI | P33219 | yebF | b1847 JW1836 | extra-cellular protein (SEC through IM, secreted in OMV), YebF | Extra-cellular (Experimental) Extra-cellular (Probable) | Outer membrane proteins OmpF, OmpC, and OmpX are central to the YebF export mechanism. Identified DsbA substrate PMID: 19953543 PMID: 16369539 Disulfide bond-containing protein PMID: 25664544 | PMID: 16369539 | Extra-cellular | X | X | N/A | Secreted | Secreted and released (Experimental) | SEC | OMV | no | yes | Y | 22 | 0.777 | SpI | 21 | 20.4773 | - | 0 | 0 | Y | n5-16c21/22o | Extracellular | 10 | 108 | ||||||
| YEHB_ECOLI | P33341 | yehB | b2109 JW2096 | Fimbrial OM b-barrel protein (SEC through IM, inserted in OM by BAM), YehB | Putative chaperone???usher fimbrial operon yeh (class ??4); Usher. Disulfide bridge (809 825) | Outer Membrane b-barrel protein | H | H | N/A | Cell outer membrane | Outer Membrane ??-barrel protein | fimbriae | SEC | BAM | no | yes | Y | 23 | 0.679 | SpI | 22 | 11.7505 | - | 0 | 0 | Y | n7-18c22/23o | OuterMembrane | 10 | 5 | |||||||
| YEHD_ECOLI | P33343 | yehD | b2111 JW2098 | Fimbrial periplasmic protein (SEC through IM), YehD | Putative chaperone???usher fimbrial operon yeh (class ??4); Fimbrial Subunit. Disulfide bond-containing protein PMID: 25664544 | Periplasmic | G | G | N/A | Periplasmic | fimbriae | SEC | no | yes | Y | 23 | 0.891 | SpI | 22 | 24.5442 | - | 1 | 0 | Y | n4-15c22/23o | Unknown | 2 | 95 | |||||||||
| NAPA_ECOLI | P33937 | napA yojC yojD yojE | yojC yojD yojE | b2206 JW2194 | periplasmic protein (TAT through IM), nitrate reductase NapA | Periplasmic (Experimental) Periplasmic (Probable) | TAT secretion system. Nitrate reductase, napFDAGHBC, is located in the periplasm. E. coli also expresses two membrane-bound proton-translocating nitrate reductases, encoded by the narGHJI and narZYWV operons. PMID: 11844760 PMID: 10234835 Disulfide bond-containing protein PMID: 25664544 | PMID: 10234835 PMID: 11844760 | Periplasmic | G | G | N/A | Periplasm | Periplasmic (Experimental) | TAT | yes | yes | Y | 32 | 0.617 | SpI | 31 | 20.6365 | - | 0 | 0 | Y | n13-23c31/32o | Periplasmic | 10 | |||||||
| AHPF_ECOLI | P35340 | ahpF | b0606 JW0599 | cytoplasmic peripheral IM protein, Alkyl hydroperoxide reductase subunit F AhpF | Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Potential) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Probable) | Experimentally detected by proteomics analysis of E.coli membrane fraction (Bernsel et al 2009). No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Potential peripherally associated protein facing the cytoplasm or integral inner membrane protein. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). PMID: 23230279 Disulfide bridge (345 348) | PMID: 19766000 PMID: 23230279 | Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm | A, F1 | A,F1 | Soluble IM Fraction | Cytoplasmic | yes | yes | N | 35 | 0.108 | CYT | 0 | -0.200913 | - | 0 | 0 | N | i | CytoplasmicMembrane | 7 | 76 | |||||||||
| DSBD_ECOLI | P36655 | dsbD cutA2 cycZ dipZ | cutA2 cycZ dipZ | b4136 JW5734 | integral IM protein (SEC in IM), Thiol:disulfide interchange DsbD | Integral Inner Membrane (Experimental) | DsbD is an inner membrane protein that is recognised and targeted to the Sec pathway by short, cleavable, N-terminal signal sequence. PMID: 15919996 Disulfide bridge (122 128;182 304;480 483) | PMID: 15919996 | in/out | Integral Inner Membrane | B | B | IM (predicted), Detected in IM Fraction | Cell inner membrane; Multi-pass membrane protein | Integral Membrane Protein (Experimental) | SRP | SEC | yes | yes | Y | 20 | 0.853 | SpI | 19 | 10.1575 | - | 8 | 7 | Y | n5-13c19/20o168-195i207-231o243-267i288-317o329-351i363-381o387-406i | CytoplasmicMembrane | 10 | |||||
| ACNB_ECOLI | P36683 | acnB yacI yacJ | yacI yacJ | b0118 JW0114 | cytoplasmic peripheral IM protein, Aconitate hydratase B AcnB | Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) | Trigger factor-sensitive aggregation. PMID: 10585860 Disulfide bond-containing protein PMID: 25664544 | PMID: 10585860 PMID: 23230279 | Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm | A, F1 | A,F1 | N/A | Cytoplasmic (Experimental) | TF | yes | yes | N | 42 | 0.124 | CYT | 0 | -0.200913 | - | 0 | 0 | N | i | Cytoplasmic | 9 | 90 | |||||||
| RLMN_ECOLI | P36979 | rlmN yfgB | yfgB | b2517 JW2501 | cytoplasmic protein, Dual-specificity RNA methyltransferase RlmN | Trigger factor-sensitive aggregation. Disulfide bridge (118 355) | Cytoplasmic | A | A | N/A | Cytoplasm | Cytoplasmic | TF | yes | yes | N | 58 | 0.101 | CYT | 0 | -0.200913 | - | 0 | 0 | N | i | Cytoplasmic | 9 | 60 | ||||||||
| BTUF_ECOLI | P37028 | btuF yadT | yadT | b0158 JW0154 | periplasmic protein (SEC through IM), Vitamin B12-binding protein BtuF | Periplasmic (Probable) | Disulfide bridge (183 259) Disulfide bond-containing protein PMID: 25664544 | PMID: 11790740 | Periplasmic | G | G | N/A | Periplasm | Periplasmic (Experimental) | SEC | yes | yes | Y | 23 | 0.751 | SpI | 22 | 10.8439 | - | 0 | 0 | Y | n8-18c22/23o | Periplasmic | 10 | |||||||
| YADN_ECOLI | P37050 | yadN | b0141 JW0137 | Fimbrial periplasmic protein (SEC through IM), YadN | Putative chaperone???usher fimbrial operon (class ??4); Fimbrial Subunit. Disulfide bridge (41 86) | Periplasmic | G | G | N/A | Fimbrium | Periplasmic | fimbriae | SEC | CU | no | yes | Y | 24 | 0.713 | SpI | 23 | 17.9817 | - | 0 | 0 | Y | n5-15c23/24o | Extracellular | 9 | 77 | |||||||
| PTKB_ECOLI | P37188 | gatB | b2093 JW2077 | cytoplasmic peripheral IM protein, PTS system galactitol-specific EIIB component GatB | Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Potential) | Part of galactitol PTS permease complex. Strong Signal Peptide prediction. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). PMID: 23230279 Disulfide bond-containing protein PMID: 25664544 | PMID: 16963640 PMID: 23230279 | Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm | A, F1 | A,F1 | N/A | Cytoplasm | Cytoplasmic | no | yes | Y | 20 | 0.743 | SpI | 19 | 8.62134 | - | 0 | 0 | Y | n5-14c19/20o | Cytoplasmic | 10 | |||||||||
| YHJJ_ECOLI | P37648 | yhjJ | b3527 JW3495 | periplasmic protein (SEC through IM), YhjJ | Periplasmic (Experimental) | Potential Skp substrate PMID: 19953543 PMID: 9298646 Disulfide bond-containing protein PMID: 25664544 | PMID: 9298646 | Periplasmic | G | G | N/A | Periplasm | Periplasmic (Experimental) | SEC | SKP | yes | yes | Y | 25 | 0.722 | SpI | 24 | 14.0569 | - | 0 | 0 | Y | n8-19c24/25o | Unknown | 2 | 83 | ||||||
| EPTB_ECOLI | P37661 | eptB yhjW | yhjW | b3546 JW5660 | integral IM protein (SEC in IM), EptB | Integral Inner Membrane (Experimental) Integral Inner Membrane (Probable) | Disulfide bond-containing protein PMID: 25664544 | PMID: 15795227 PMID: 15919996 | in/out | Integral Inner Membrane | B | B | IM (predicted) | Cell inner membrane | Integral Membrane Protein (Experimental) | SRP | SEC | yes | yes | N | 38 | 0.136 | TMH | 0 | 11.408 | - | 5 | 5 | N | i12-30o42-65i77-103o123-140i161-178o | CytoplasmicMembrane | 10 | |||||
| YIBQ_ECOLI | P37691 | yibQ | b3614 JW5645 | periplasmic protein (SEC through IM), YibQ | Periplasmic | G | G | N/A | Periplasmic | SEC | yes | yes | Y | 24 | 0.782 | SpI | 23 | 13.9145 | - | 1 | 0 | Y | n8-18c23/24o | Unknown | 2 | 14 | |||||||||||
| WBBK_ECOLI | P37751 | wbbK yefI | yefI | b2032 JW2017 | cytoplasmic peripheral IM protein, Putative glycosyltransferase WbbK | Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) | Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). PMID: 23230279 Disulfide bond-containing protein PMID: 25664544 | PMID: 23230279 | Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm | A, F1 | A,F1 | Soluble IM Fraction | Cell inner membrane | Cytoplasmic | no | yes | N | 21 | 0.119 | CYT | 0 | -0.200913 | - | 0 | 0 | N | i | Unknown | 2 | 32 | |||||||
| GLTI_ECOLI | P37902 | gltI ybeJ yzzK | ybeJ yzzK | b0655 JW5092 | periplasmic protein (SEC through IM), Glutamate/aspartate import solute-binding protein GltI | Periplasmic (Experimental) | Substrate of periplasmic disulfide oxidoreductases DsbA. Identified DsbA substrate PMID: 19953543 PMID: 8740179 Potential substrate for the SRP pathway. It was found to be down-regulated in the periplasmic compartment of SRP-depleted cells. PMID: 22030350 Disulfide bond-containing protein PMID: 25664544 | PMID: 22030350 PMID: 8740179 | Periplasmic | G | G | N/A | Periplasm | Periplasmic (Experimental) | SEC | DSBA | yes | yes | Y | 23 | 0.775 | SpI | 22 | 21.5903 | - | 0 | 0 | Y | n7-17c22/23o | Periplasmic | 10 | 93 | |||||
| YBGD_ECOLI | P37909 | ybgD | b0719 JW0709 | Fimbrial periplasmic protein (SEC through IM), YbgD | Putative chaperone???usher fimbrial operon yeh (class ??); Fimbrial Subunit. Disulfide bridge (42 81) | Periplasmic | G | G | N/A | Fimbrium | Periplasmic | fimbriae | SEC | CU | no | yes | Y | 24 | 0.878 | SpI | 23 | 15.4399 | - | 0 | 0 | Y | n7-18c23/24o | Extracellular | 9 | ||||||||
| SFMF_ECOLI | P38052 | sfmF ybcG | ybcG | b0534 JW5072 | Fimbrial periplasmic protein (SEC through IM), SfmF | Potential fimbrial sfm operon (class ??1); Fimbrial Subunit. Disulfide bridge (40 80) | Periplasmic | G | G | N/A | Fimbrium | Periplasmic | fimbriae | SEC | CU | no | yes | Y | 21 | 0.763 | SpI | 20 | 16.3129 | - | 0 | 0 | Y | n4-15c20/21o | Extracellular | 9 | 64 | ||||||
| TORT_ECOLI | P38683 | torT yccH | yccH | b0994 JW0979 | periplasmic protein (SEC through IM), Periplasmic TorT | Periplasmic (Experimental) | Might be is co-translationally translocated through Sec translocon. PMID: 8576063 The precursor of TorT harbouring hydrophobic signal sequence require SRP and FtsY in addition to SecA for maximal translocation. PMID: 24717922 Potential substrate for the SRP pathway. When fused to cytoplasmic protein thioredoxin promotes signal recognition particle-dependent translocation PMID: 15838024 Disulfide bond-containing protein PMID: 25664544 | PMID: 15838024 PMID: 24717922 PMID: 8576063 | Periplasmic | G | G | N/A | Periplasm | Periplasmic (Experimental) | SRP (exp) | SEC | yes | yes | Y | 19 | 0.789 | SpI | 18 | 12.9296 | - | 0 | 0 | Y | n3-14c18/19o | Periplasmic | 10 | 12 | |||||
| ZNUA_ECOLI | P39172 | znuA yebL yzzP | yebL yzzP | b1857 JW5831 | periplasmic protein (SEC through IM), High-affinity zinc uptake system ZnuA | Periplasmic (Experimental) | Disulfide bond-containing protein PMID: 25664544 | PMID: 9298646 | Periplasmic | G | G | N/A | Periplasm | Periplasmic (Experimental) | SEC | yes | yes | Y | 27 | 0.573 | SpI | 23 | 18.3411 | - | 0 | 0 | Y | n6-17c26/27o | Periplasmic | 10 | 84 | ||||||
| FIMI_ECOLI | P39264 | fimI | b4315 JW5779 | Fimbrial periplasmic protein (SEC through IM), Fimbrin-like FimI | Fim operon (class ??1); Fimbrial Subunit. Required for the regulation of length and mediation of adhesion of Escherichia coli type 1 fimbriae. Disulfide bridge (43 83) | Periplasmic | G | G | N/A | Fimbrium | Periplasmic | fimbriae | SEC | CU | no | yes | Y | 20 | 0.716 | SpI | 19 | 16.0857 | - | 0 | 0 | Y | n6-13c19/20o | Extracellular | 9 | 37 | |||||||
| YTFQ_ECOLI | P39325 | ytfQ | b4227 JW4186 | periplasmic protein (SEC through IM), ABC transporter periplasmic-binding YtfQ | Periplasmic (Experimental) | Identified DsbA substrate PMID: 19953543 PMID: 9298646 Disulfide bond-containing protein PMID: 25664544 | PMID: 9298646 | Periplasmic | G | G | N/A | Periplasm | Periplasmic (Experimental) | SEC | DSBA | yes | yes | Y | 22 | 0.849 | SpI | 21 | 21.6548 | - | 1 | 0 | Y | n5-13c21/22o | Periplasmic | 10 | 90 | ||||||
| OPGB_ECOLI | P39401 | mdoB opgB yjjO | opgB yjjO | b4359 JW5794 | integral IM protein (SEC in IM), Phosphoglycerol transferase I MdoB | Integral Inner Membrane (Probable) | Disulfide bond-containing protein PMID: 25664544 | PMID: 15919996 | out/out | Integral Inner Membrane | B | B | IM (predicted) | Cell inner membrane | Integral Membrane Protein | SRP | SEC | no | yes | N | 21 | 0.198 | TMH | 0 | 5.71979 | - | 4 | 4 | Y | n4-14c18/19o28-47i59-74o80-98i110-128o | CytoplasmicMembrane | 10 | 7 | ||||
| RIR3_ECOLI | P39452 | nrdE | b2675 JW2650 | cytoplasmic protein, Ribonucleoside-diphosphate reductase 2 subunit alpha NrdE | Disulfide bridge (178 415) | Cytoplasmic | A | A | N/A | Cytoplasmic | yes | yes | N | 30 | 0.107 | CYT | 0 | -0.200913 | - | 0 | 0 | N | i | Cytoplasmic | 9 | 7 | |||||||||||
| YGIL_ECOLI | P39834 | ygiL | b3043 JW3011 | Fimbrial periplasmic protein (SEC through IM), YgiL | Putative chaperone???usher fimbrial operon (class ??); Unknown function. Disulfide bridge (41 80) | Periplasmic | G | G | N/A | Fimbrium | Periplasmic | fimbriae | SEC | CU | no | yes | Y | 24 | 0.867 | SpI | 23 | 15.5994 | - | 0 | 0 | Y | n7-18c23/24o | Extracellular | 9 | 92 | |||||||
| OPGD_ECOLI | P40120 | mdoD opgD ydcG yzzZ | opgD ydcG yzzZ | b1424 JW1420 | periplasmic protein (TAT-SEC through IM), Glucans biosynthesis D MdoD | Periplasmic (Experimental) | TAT secretion system. PMID: 9298646 Disulfide bond-containing protein PMID: 25664544 | PMID: 9298646 | Periplasmic | G | G | N/A | Periplasm | Periplasmic (Experimental) | TAT,SEC | no | yes | Y | 33 | 0.683 | SpI | 32 | 18.7932 | - | 0 | 0 | Y | n14-25c32/33o | Periplasmic | 10 | 61 | ||||||
| YRAH_ECOLI | P42913 | yraH | b3142 JW3111 | Fimbrial periplasmic protein (SEC through IM), YraH | Potential fimbrial yra operon (class ??1); Fimbrial Subunit. Disulfide bridge (38 78) | Periplasmic | G | G | N/A | Fimbrium | Periplasmic | fimbriae | SEC | CU | no | yes | Y | 23 | 0.673 | SpI | 22 | 11.7644 | - | 0 | 0 | Y | n7-17c22/23o | Extracellular | 9 | ||||||||
| YRAJ_ECOLI | P42915 | yraJ | b3144 JW3113 | Fimbrial OM b-barrel protein (SEC through IM, inserted in OM by BAM), YraJ | Potential fimbrial yra operon (class ??1); Usher. Disulfide bridge (815 837) | Outer Membrane b-barrel protein | H | H | N/A | Cell outer membrane | Outer Membrane ??-barrel protein | fimbriae | SEC | BAM | no | yes | N | 41 | 0.51 | SpI | 40 | 9.33083 | - | 0 | 0 | N | i | OuterMembrane | 10 | 6 | |||||||
| YHCD_ECOLI | P45420 | yhcD | b3216 JW3183 | Fimbrial OM b-barrel protein (SEC through IM, inserted in OM by BAM), YhcD | Potential fimbrial yhc operon (class ??); Uknown function. Disulfide bridge (769 792) | Outer Membrane b-barrel protein | H | H | N/A | Cell outer membrane | Outer Membrane ??-barrel protein | fimbriae | SEC | BAM | no | yes | Y | 22 | 0.809 | SpI | 21 | 10.0887 | - | 0 | 0 | Y | n5-16c21/22o | OuterMembrane | 10 | ||||||||
| LPOA_ECOLI | P45464 | lpoA yraM | yraM | b3147 JW3116 | OM lipoprotein (SEC through IM), inserted in OM by LOL), Penicillin-binding activator LpoA | Outer Membrane Lipoprotein (Experimental) Outer Membrane Lipoprotein (Probable) | The two OM lipoproteins, LpoA and LpoB, are essential for the function, respectively, of PBP1A and PBP1B, the major E. coli bifunctional peptidoglycans (PG) synthases. Each Lpo protein binds specifically to its cognate PBP and stimulates its transpeptidase. Outer membrane protein determined by 2D gel electrophoresis (Lopez-Campistrous et al 2005) Disulfide bond-containing protein PMID: 25664544 | PMID: 15911532 PMID: 20932056 PMID: 21183073 PMID: 21183074 | Outer Membrane Lipoprotein | I | I | Soluble IM Fraction | Cell outer membrane | Membrane Lipoprotein | SEC | LOL | no | yes | Y | 26 | 0.162 | SpII | 26 | 12.6937 | Pos+2=G | 0 | 0 | Y | n14-27c45/46o | CytoplasmicMembrane | 9 | 105 | |||||
| AIS_ECOLI | P45565 | ais pmrG | pmrG | b2252 JW2246 | periplasmic protein (SEC through IM), polysaccharide core heptose Ais | Periplasmic ( By similarity) | Periplasmic protein by virtue of its homology with the well characterized Salmonella homologue PmrGPMID: 16803591. Disulfide bond-containing protein PMID: 25664544 | PMID: 16803591 | Periplasmic | G | G | N/A | Periplasm | Membrane anchored | SEC | yes | yes | N | 32 | 0.351 | SpI | 31 | 9.81523 | - | 1 | 0 | Y | n16-27c31/32o | Unknown | 2 | 50 | ||||||
| LUXS_ECOLI | P45578 | luxS ygaG | ygaG | b2687 JW2662 | cytoplasmic peripheral IM protein, S-ribosylhomocysteine lyase LuxS | Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) | Copurifies with trigger factor in pull down assays. Disulfide bond-containing protein PMID: 25664544 | PMID: 23230279 | Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm | A, F1 | A,F1 | N/A | Cytoplasmic | TF | no | yes | N | 19 | 0.106 | CYT | 0 | -0.200913 | - | 0 | 0 | N | i | Cytoplasmic | 8 | 110 | |||||||
| YHDW_ECOLI | P45766 | yhdW | b3268 JW3236 | periplasmic protein (SEC through IM), Putative amino-acid ABC transporter-binding YhdW | Disulfide bond-containing protein PMID: 25664544 | Periplasmic | G | G | Soluble IM Fraction | Periplasm | Cytoplasmic | SEC | pseudogene (Uniprot) | no | no | Y | 25 | 0.628 | SpI | 24 | 20.0416 | - | 0 | 0 | Y | n6-17c24/25o | Periplasmic | 10 | 89 | ||||||||
| YBHC_ECOLI | P46130 | ybhC | b0772 JW0755 | OM lipoprotein (SEC through IM), inserted in OM by LOL), Putative acyl-CoA thioester hydrolase YbhC | Outer Membrane Lipoprotein (Experimental) | Proven pignal peptidase II substrate. Outer membrane protein determined by 2D gel electrophoresis (Lopez-Campistrous et al 2005, Molloy et al 2000) PMID: 10806384 Disulfide bridge (185 197) PMID: 19452549 | PMID: 10806384 PMID: 15174130 PMID: 15911532 PMID: 20932056 | Outer Membrane Lipoprotein | I | I | Soluble IM Fraction | Cell outer membrane | Outer membrane Lipoprotein (Experimental) | SEC | LOL | yes | yes | Y | 24 | 0.274 | SpII | 21 | 24.9976 | Pos+2=S | 0 | 0 | Y | n9-19c24/25o | OuterMembrane | 9 | 71 | ||||||
| CSGC_ECOLI | P52107 | csgC | b1043 JW1026 | periplasmic protein (SEC through IM), Curli assembly CsgC | Uniprot mentions an 8aa signal peptide but Signal P prediction is for a "typical" 22 residue peptide Disulfide bond-containing protein PMID: 25664544 Disulfide bond-containing protein PMID: 25664544 | Periplasmic | G | G | N/A | Periplasm | Periplasmic | SEC | no | yes | N | 22 | 0.179 | SpI | 22 | 2.07115 | - | 0 | 0 | Y | n4-16c21/22o | Unknown | 2 | 68 | |||||||||
| RZOD_ECOLI | P58041 | rzoD | b4510 JW5080 | OM lipoprotein (SEC through IM), inserted in OM by LOL), Prophage RzoD (o-spanin) | Disulfide bridge (29 29) | Outer Membrane Lipoprotein | I | I | N/A | Cell outer membrane | Outer membrane Lipoprotein | SEC | LOL | Prophage Integrase (EcoGene) | no | no | Y | 20 | 0.187 | SpII | 19 | 19.9508 | Pos+2=T | 0 | 0 | Y | n8-18c22/23o | Unknown | 2 | 147 | |||||||
| RZOR_ECOLI | P58042 | rzoR | b4528 JW5213 | OM lipoprotein (SEC through IM), inserted in OM by LOL), Prophage RzoR (o-spanin) | Disulfide bridge (29 29) | Outer Membrane Lipoprotein | I | I | N/A | Cell outer membrane | Outer membrane Lipoprotein | SEC | LOL | Prophage Integrase (EcoGene) | no | no | Y | 20 | 0.189 | SpII | 19 | 20.8844 | Pos+2=T | 0 | 0 | Y | n8-18c22/23o | Unknown | 2 | 88 | |||||||
| YEBY_ECOLI | P64506 | yebY | b1839 JW1828 | periplasmic protein (SEC through IM), YebY | Identified DsbA substrate PMID: 19953543 | Periplasmic | G | G | N/A | Periplasmic | SEC | DSBA | yes | yes | Y | 21 | 0.862 | SpI | 20 | 19.6801 | - | 1 | 0 | Y | n5-12c20/21o | Unknown | 2 | 35 | |||||||||
| YFIR_ECOLI | P64548 | yfiR | b2603 JW2584 | periplasmic protein (SEC through IM), YfiR | Disulfide bond-containing protein PMID: 25664544 | Periplasmic | G | G | N/A | Periplasm | Periplasmic | SEC | yes | yes | Y | 23 | 0.684 | SpI | 22 | 13.4131 | - | 0 | 0 | Y | n7-17c22/23o | Unknown | 6 | 21 | |||||||||
| DPAL_ECOLI | P66899 | ygeX | b2871 JW2839 | cytoplasmic protein, Diaminopropionate ammonia-lyase YgeX | Disulfide bridge (265 291) PMID: 22505717 | Cytoplasmic | A | A | N/A | Cytoplasmic | yes | yes | N | 33 | 0.114 | CYT | 0 | -0.200913 | - | 0 | 0 | N | i | Cytoplasmic | 9 | 75 | |||||||||||
| GLRX1_ECOLI | P68688 | grxA grx | grx | b0849 JW0833 | cytoplasmic protein, Glutaredoxin 1 GrxA | Disulfide bridge (11 14) | Cytoplasmic | A | A | N/A | Cytoplasmic | yes | yes | N | 18 | 0.204 | CYT | 0 | -0.200913 | - | 0 | 0 | N | i | Unknown | 2 | |||||||||||
| RCSF_ECOLI | P69411 | rcsF | b0196 JW0192 | OM lipoprotein (SEC through IM, inserted in OM by LOL), RcsF | Outer Membrane Lipoprotein (Experimental) Outer Membrane Lipoprotein (Probable) | Proven pignal peptidase II substrate. Identified DsbA substrate PMID: 19953543 PMID: 15174130 Disulfide bridge (74 118;109 124) Disulfide bond-containing protein PMID: 25664544 | PMID: 15174130 PMID: 16740933 PMID: 20932056 PMID: 23813676 | Outer Membrane Lipoprotein | I | I | Soluble IM Fraction | Cell outer membrane | Outer membrane Lipoprotein (Experimental) | SEC | DSBA | LOL | yes | yes | Y | 23 | 0.182 | SpII | 15 | 13.2891 | Pos+2=S | 0 | 0 | Y | n3-13c21/22o | OuterMembrane | 10 | 81 | |||||
| YAHK_ECOLI | P75691 | yahK | b0325 JW0317 | cytoplasmic peripheral IM protein, Aldehyde reductase YahK | Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Experimental) Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm (Potential) | No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. No transmembrane helix is predicted. Weak hydrophobic and non cytoplasmic regions predicted by Phobius. Detected in acossiation with the inner membrane (sequential biochemical fractionations coupled with high resolution mass spectrometry). PMID: 23230279 Disulfide bond-containing protein PMID: 25664544 | PMID: 23230279 | Cytoplasmic, Peripheral inner membrane protein facing the cytoplasm | A, F1 | A,F1 | IM (predicted-detected) | Cytoplasmic | no | yes | N | 53 | 0.148 | CYT | 0 | -0.200913 | - | 0 | 0 | N | i | Cytoplasmic | 9 | 73 | |||||||||
| YBGO_ECOLI | P75748 | ybgO | b0716 JW5098 | Fimbrial periplasmic protein (SEC through IM), YbgO | Putative chaperone???usher fimbrial operon yeh (class ??); Unkown function. Disulfide bond-containing protein PMID: 25664544 | Periplasmic | G | G | N/A | Periplasmic | fimbriae | SEC | no | yes | N | 24 | 0.359 | SpI | 23 | 7.76951 | - | 0 | 0 | Y | n7-18c23/24o | Cytoplasmic | 8 | 53 | |||||||||
| YBGQ_ECOLI | P75750 | ybgQ | b0718 JW5099 | Fimbrial OM b-barrel protein (SEC through IM, inserted in OM by BAM), YbgQ | Putative chaperone???usher fimbrial operon yeh (class ??); Usher. Disulfide bridge (795 814) | Outer Membrane b-barrel protein | H | H | N/A | Cell outer membrane | Outer Membrane ??-barrel protein | fimbriae | SEC | BAM | no | yes | Y | 22 | 0.84 | SpI | 21 | 8.29107 | - | 0 | 0 | Y | n6-16c21/22o | OuterMembrane | 10 | 10 | |||||||
| FIU_ECOLI | P75780 | fiu ybiL | ybiL | b0805 JW0790 | OM b-barrel protein (SEC through IM, inserted in OM by BAM), Catecholate siderophore receptor Fiu | Outer Membrane b-barrel protein (Experimental) | Outer membrane protein determined by 2D gel electrophoresis (Lopez-Campistrous et al 2005, Fountoulakis et al 2003)','Outer membrane protein determined by 2D gel electrophoresis (Lopez-Campistrous et al 2005, Fountoulakis et al 2003, Molloy et al 2000) Potential Skp substrate PMID: 19953543 PMID: 10806384 Disulfide bond-containing protein PMID: 25664544 | PMID: 10806384 PMID: 20932056 | Outer Membrane b-barrel protein | H | H | N/A | Cell outer membrane | Outer Membrane ??-barrel protein (Experimental) | SEC | SKP | BAM | yes | yes | Y | 34 | 0.525 | SpI | 33 | 11.8291 | - | 1 | 0 | Y | n14-25c33/34o | OuterMembrane | 10 | 10 | ||||
| YBJP_ECOLI | P75818 | ybjP | b0865 JW0849 | OM lipoprotein (SEC through IM, inserted in OM by LOL), YbjP | Outer Membrane Lipoprotein (Experimental) | Proven pignal peptidase II substrate. Outer membrane protein determined by 2D gel electrophoresis (Lopez-Campistrous et al 2005) Identified in nucleoids collected by sucrose gradient centrifugation and analyzed by liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS). PMID: 21541338 Identified DsbA substrate PMID: 19953543 PMID: 10806384 Disulfide bond-containing protein PMID: 25664544 | PMID: 10806384 PMID: 15174130 PMID: 15911532 PMID: 20932056 | Outer Membrane Lipoprotein | I | I | Soluble IM Fraction | Cell membrane | Outer membrane Lipoprotein (Experimental) | SEC | DSBA | LOL | no | yes | Y | 28 | 0.445 | SpII | 18 | 10.6734 | Pos+2=T | 0 | 0 | Y | n9-19c24/25o | Unknown | 2 | 69 | |||||
| YCGJ_ECOLI | P76001 | ycgJ | b1177 JW1166 | periplasmic protein (SEC through IM), YcgJ | Disulfide bond-containing protein PMID: 25664544 | Periplasmic | G | G | N/A | Periplasmic | SEC | yes | yes | Y | 23 | 0.404 | SpI | 22 | 8.58297 | - | 0 | 0 | Y | n6-16c24/25o | Unknown | 2 | 77 | ||||||||||
| YDCS_ECOLI | P76108 | ydcS | b1440 JW1435 | periplasmic protein (SEC through IM), Bifunctional polyhydroxybutyrate synthase / ABC transporter periplasmic binding YdcS | Periplasmic (Experimental) | Potential substrate for the SRP pathway. It was found to be down-regulated in the periplasmic compartment of SRP-depleted cells. PMID: 22030350 Disulfide bond-containing protein PMID: 25664544 | PMID: 15911532 PMID: 22030350 | Periplasmic | G | G | N/A | Periplasm | Periplasmic (Experimental) | SEC | no | yes | Y | 23 | 0.836 | SpI | 22 | 17.8592 | - | 0 | 0 | Y | n10-18c22/23o | Periplasmic | 10 | 16 | |||||||
| YNFB_ECOLI | P76170 | ynfB | b1583 JW1575 | periplasmic peripheral OM protein (SEC through IM), UPF0482 YnfB | Peripheral outer membrane protein facing the periplasm (Experimental) | Outer membrane protein determined by 2D gel electrophoresis (Lopez-Campistrous et al 2005) Disulfide bond-containing protein PMID: 25664544 | PMID: 15911532 | Peripheral outer membrane protein facing the periplasm | F3 | F3 | N/A | Periplasmic | SEC | yes | yes | Y | 29 | 0.87 | SpI | 28 | 19.5483 | - | 0 | 0 | Y | n9-20c28/29o | Unknown | 2 | 49 | ||||||||
| YNJH_ECOLI | P76227 | ynjH | b1760 JW1749 | periplasmic protein (SEC through IM), YnjH | Disulfide bond-containing protein PMID: 25664544 | Periplasmic | G | G | N/A | Periplasmic | SEC | no | yes | Y | 19 | 0.569 | SpI | 18 | 11.8884 | - | 0 | 0 | Y | n4-14c18/19o | CytoplasmicMembrane | 9 | 27 | ||||||||||
| MSRC_ECOLI | P76270 | msrC yebR | yebR | b1832 JW1821 | cytoplasmic protein, Free methionine-R-sulfoxide reductase MsrC | Disulfide bond-containing protein PMID: 25664544 | Cytoplasmic | A | A | N/A | Cytoplasmic | no | yes | N | 20 | 0.13 | CYT | 0 | -0.200913 | - | 0 | 0 | N | i | Unknown | 2 | 57 | ||||||||||
| FLHE_ECOLI | P76297 | flhE | b1878 JW1867 | periplasmic protein (SEC through IM), FlhE | Part of the flagellar export apparatus Disulfide bond-containing protein PMID: 25664544 | Periplasmic | G | G | N/A | Periplasmic | SEC | Fla | yes | yes | Y | 17 | 0.511 | SpI | 16 | 8.24229 | - | 0 | 0 | Y | n3-14c26/27o | Unknown | 2 | 68 | |||||||||
| ZINT_ECOLI | P76344 | zinT yodA | yodA | b1973 JW1956 | periplasmic protein (SEC through IM), Metal-binding ZinT | Periplasmic (Experimental) Periplasmic (Probable) | After 45 min of cadmium exposure the YodA protein was primarily detected in the cytoplasmic fraction but was later (150 min) found in both the cytoplasmic and periplasmic compartments. PMID: 9579078 Disulfide bond-containing protein PMID: 25664544 | PMID: 12480884 PMID: 9579078 | Periplasmic | G | G | N/A | Cytoplasm | Periplasmic (Experimental) | SEC | yes | yes | Y | 24 | 0.683 | SpI | 23 | 12.2997 | - | 0 | 0 | Y | n8-18c23/24o | Unknown | 2 | |||||||
| YFCP_ECOLI | P76499 | yfcP | b2333 JW2330 | Fimbrial periplasmic protein (SEC through IM), YfcP | Putative chaperone???usher fimbrial operon yfc (class ??); Fimbrial Subunit. Disulfide bridge (43 83) | Periplasmic | G | G | N/A | Fimbrium | Periplasmic | fimbriae | SEC | CU | yes | yes | Y | 28 | 0.79 | SpI | 27 | 14.214 | - | 1 | 0 | Y | n8-19c27/28o | Unknown | 2 | ||||||||
| YFEY_ECOLI | P76537 | yfeY | b2432 JW2425 | OM lipoprotein (SEC through IM, inserted in OM by LOL), YfeY | Outer Membrane Lipoprotein (Experimental) | Proven pignal peptidase II substrate. Outer membrane protein determined by LC-MS (Gevaert et al 2002) PMID: 15174130 Disulfide bond-containing protein PMID: 25664544 | PMID: 15174130 PMID: 20932056 | Outer Membrane Lipoprotein | I | I | N/A | Outer membrane Lipoprotein (Experimental) | SEC | LOL | yes | yes | Y | 29 | 0.248 | SpII | 17 | 20.3796 | Pos+2=S | 0 | 0 | Y | n8-19c27/28o | Unknown | 2 | 59 | |||||||
| YPFG_ECOLI | P76559 | ypfG | b2466 JW2450 | periplasmic protein (SEC through IM), YpfG | Disulfide bond-containing protein PMID: 25664544 | Periplasmic | G | G | N/A | Periplasmic | SEC | yes | yes | Y | 22 | 0.804 | SpI | 21 | 15.2807 | - | 1 | 0 | Y | n5-16c21/22o | CytoplasmicMembrane | 9 | 61 | ||||||||||
| YFHM_ECOLI | P76578 | yfhM | b2520 JW2504 | IM lipoprotein (SEC through IM), inserted in OM by LOL), Alpha-2-macroglobulin YfhM | Identified DsbA substrate PMID: 19953543 | Inner Membrane Lipoprotein | E | E | Soluble IM Fraction | Cell inner membrane | Inner membrane lipoprotein | SEC | DSBA | LOL | yes | yes | Y | 25 | 0.18 | SpII | 17 | 28.7232 | Pos+2=D | 0 | 0 | Y | n6-13c18/19o | Unknown | 4 | ||||||||
| YQIG_ECOLI | P76655 | yqiG | b3046 JW5507 | Fimbrial OM b-barrel protein (SEC through IM, inserted in OM by BAM), Putative usher YqiG | Putative chaperone???usher fimbrial operon (class ??); Usher. Disulfide bridge (798 817) | Outer Membrane b-barrel protein | H | H | N/A | Cell outer membrane | Outer Membrane ??-barrel protein | fimbriae | SEC | BAM | pseudogene (Uniprot) | no | yes | N | 21 | 0.333 | CYT | 0 | -0.200913 | - | 0 | 0 | N | i | OuterMembrane | 10 | |||||||
| YKFB_ECOLI | P77162 | ykfB | b0250 JW0239 | periplasmic protein (SEC through IM), YkfB | Disulfide bond-containing protein PMID: 25664544 | Periplasmic | G | G | N/A | Periplasmic | SEC | Prophage Integrase (EcoGene) | no | no | Y | 24 | 0.716 | SpI | 23 | 17.8556 | - | 0 | 0 | Y | n11-19c23/24o | Unknown | 2 | 59 | |||||||||
| DSBG_ECOLI | P77202 | dsbG ybdP | ybdP | b0604 JW0597 | periplasmic protein (SEC through IM), Thiol:disulfide interchange DsbG | Periplasmic (Experimental) | Disulfide bridge (126 129) PMID: 15184683 Disulfide bond-containing protein PMID: 25664544 | PMID: 9383195 | Periplasmic | G | G | N/A | Periplasm | Periplasmic (Experimental) | SEC | yes | yes | Y | 18 | 0.618 | SpI | 17 | 12.1105 | - | 0 | 0 | Y | n5-12c17/18o | Periplasmic | 10 | 50 | ||||||
| RCLA_ECOLI | P77212 | rclA ykgC | ykgC | b0304 JW5040 | cytoplasmic protein, Probable pyridine nucleotide-disulfide oxidoreductase RclA | Disulfide bridge (43 48) | Cytoplasmic | A | A | N/A | Cytoplasmic | no | yes | N | 20 | 0.197 | CYT | 0 | -0.200913 | - | 0 | 0 | N | i | Cytoplasmic | 9 | 46 | ||||||||||
| ECPE_ECOLI | P77263 | ecpE matF yagV | matF yagV | b0289 JW5030 | Fimbrial periplasmic protein (SEC through IM), Probable chaperone EcpE | Putative chaperone???usher fimbrial operon yag(class ??); Unknown function. Potential substrate of periplasmic peptidyl prolyl cis???trans isomerase SurA; sequence contains known binding motif of SurA [RYEF]X[RYEF][P]. Disulfide bond-containing protein PMID: 25664544 | Periplasmic | G | G | N/A | Periplasmic | fimbriae | SEC | SURA | no | yes | Y | 28 | 0.534 | SpI | 27 | 8.52618 | - | 1 | 0 | Y | n12-22c27/28o | Unknown | 2 | 17 | |||||||
| YPHF_ECOLI | P77269 | yphF | b2548 JW2532 | periplasmic protein (SEC through IM), ABC transporter periplasmic-binding YphF | Periplasmic (Experimental) | Disulfide bond-containing protein PMID: 25664544 | PMID: 9298646 | Periplasmic | G | G | N/A | Periplasm | Periplasmic (Experimental) | SEC | no | yes | Y | 27 | 0.817 | SpI | 26 | 20.8859 | - | 0 | 0 | Y | n11-22c26/27o | Periplasmic | 9 | 63 | |||||||
| RSXG_ECOLI | P77285 | rsxG rnfG ydgP | rnfG ydgP | b1631 JW1623 | periplasmic protein (SEC through IM), Ion-translocating oxidoreductase complex subunit G RsxG | Disulfide bond-containing protein PMID: 25664544 | Periplasmic | G | G | N/A | Cell inner membrane | Periplasmic | SEC | yes | yes | Y | 25 | 0.427 | SpI | 24 | 11.9411 | - | 0 | 0 | Y | n9-17c24/25o | Unknown | 2 | 42 | ||||||||
| YDER_ECOLI | P77294 | ydeR | b1503 JW1497 | Fimbrial periplasmic protein (SEC through IM), YdeR | Potential fimbrial yde operon (class ??1); Fimbrial Subunit. Disulfide bridge (39 77) | Periplasmic | G | G | N/A | Fimbrium | Periplasmic | fimbriae | SEC | CU | no | yes | Y | 24 | 0.701 | SpI | 23 | 20.0135 | - | 0 | 0 | Y | n8-18c23/24o | Unknown | 2 | 67 | |||||||
| BORD_ECOLI | P77330 | borD ybcU | ybcU | b0557 JW0546 | OM lipoprotein (SEC through IM), inserted in OM by LOL), Prophage Bor homolog BorD | Outer Membrane Lipoprotein (Experimental) | Proven pignal peptidase II substrate. PMID: 15174130 Disulfide bond-containing protein PMID: 25664544 | PMID: 15174130 | Outer Membrane Lipoprotein | I | I | Soluble IM Fraction | Cell membrane | Outer membrane Lipoprotein (Experimental) | SEC | LOL | Prophage Integrase (EcoGene) | no | no | Y | 21 | 0.303 | SpII | 16 | 21.3732 | Pos+2=A | 0 | 0 | Y | n5-14c18/19o | Unknown | 2 | 32 | ||||
| HLYE_ECOLI | P77335 | hlyE clyA hpr sheA ycgD | clyA hpr sheA ycgD | b1182 JW5181 | periplasmic & extra-cellular protein (SEC through IM), secreted in OMV), Hemolysin E, chromosomal HlyE | Periplasmic, Extra-cellular (Experimental) Periplasmic, Extra-cellular (Potential) Periplasmic, Extra-cellular (Probable) | HlyE(ClyA) forms a dodecameric transmembrane pore. The ability of ClyA to translocate to the periplasm is abolished in deletion mutants lacking the last 23 or 11 amino acid residues of the C-terminal region. Also, analysis of ClyA mutants with defined deletions and amino acid exchanges revealed that several ClyA segments which are distant of each other in the primary structure, namely, the N-terminal ??A1 region, the C-terminal region, and the hydrophobic sequence ranging from residues 89 to 101, are crucial for ClyA translocation from the E. coli cytoplasm to the periplasm. In the soluble ClyA monomer, these regions are found closely adjacent to each other in the tail domain. ClyA assembles into an oligomeric structure in solution in the absence of either bilayer membranes or detergents at physiological temperature.These oligomers can rearrange to create transmembrane pores when in contact with detergents or biological membranes. ClyA does not form transmembrane pores on E. coli lipid membranes. Recombinant E. coli overexpressing the cloned clyA gene accumulated this haemolysin in the periplasm and released only very small amounts of it into the external medium. PMID: 11902713 Disulfide bridge (87 285) PMID: 14532000 | PMID: 10027972 PMID: 11902713 PMID: 20511497 | Periplasmic, Extra-cellular | G, X | G,X | N/A | Secreted Periplasm Host cell membrane | Secreted and released (Experimental) | SEC | OMV | no | yes | N | 58 | 0.117 | CYT | 0 | -0.200913 | - | 1 | 0 | N | i | Extracellular | 10 | 62 | |||||
| YBCL_ECOLI | P77368 | ybcL | b0545 JW0533 | periplasmic protein (SEC through IM), UPF0098 YbcL | Periplasmic (Probable) | Disulfide bridge (46 129) Disulfide bond-containing protein PMID: 25664544 | PMID: 11439028 | Periplasmic | G | G | N/A | Periplasm | Periplasmic (Experimental) | SEC | Prophage Integrase (EcoGene) | yes | no | Y | 20 | 0.717 | SpI | 19 | 19.8157 | - | 0 | 0 | Y | n4-15c19/20o | Periplasmic | 10 | 48 | ||||||
| RCLR_ECOLI | P77379 | rclR ykgD | ykgD | b0305 JW0298 | nucleoid protein, RCS-specific HTH-type transcriptional activator RclR | Cytoplasmic, Nucleoid (Experimental) | Disulfide bridge (21 89) PMID: 24078635 Disulfide bridge (21 89) PMID: 24078635 | PMID: 23138451 | Cytoplasmic, Nucleoid | A, N | A,N | IM (predicted) | Cytoplasmic | no | yes | N | 34 | 0.112 | CYT | 0 | -0.200913 | - | 0 | 0 | N | i | Unknown | 2 | 13 | ||||||||
| SFMD_ECOLI | P77468 | sfmD | b0532 JW0521 | Fimbrial OM b-barrel protein (SEC through IM, inserted in OM by BAM), SfmD | Potential fimbrial sfm operon (class ??1); Usher. Disulfide bridge (840 862) | Outer Membrane b-barrel protein | H | H | N/A | Cell outer membrane | Outer Membrane ??-barrel protein | fimbriae | SEC | BAM | yes | yes | N | 36 | 0.404 | TMH | 0 | 3.9822 | - | 1 | 1 | N | i12-32o | OuterMembrane | 10 | ||||||||
| YAIW_ECOLI | P77562 | yaiW | b0378 JW0369 | OM lipoprotein (SEC through IM, inserted in OM by LOL), YaiW | Outer Membrane Lipoprotein (Experimental) | Disulfide bond-containing protein PMID: 25664544 | PMID: 20932056 | Outer Membrane Lipoprotein | I | I | N/A | Outer membrane Lipoprotein | SEC | LOL | no | yes | Y | 26 | 0.354 | SpII | 20 | 28.9818 | Pos+2=S | 0 | 0 | Y | n7-18c23/24o | Unknown | 2 | 10 | |||||||
| YKGA_ECOLI | P77601 | ykgA | b0300 JW5037 | nucleoid protein, Putative HTH-type transcriptional regulator YkgA | Cytoplasmic, Nucleoid (Experimental) | Disulfide bond-containing protein PMID: 25664544 | PMID: 23138451 | Cytoplasmic, Nucleoid | A, N | A,N | N/A | Cytoplasmic | pseudogene (Uniprot), pseudogene (Ochman & Davalos, 2006) | no | no | N | 56 | 0.1 | CYT | 0 | -0.200913 | - | 0 | 0 | N | i | Cytoplasmic | 9 | 14 | ||||||||
| THII_ECOLI | P77718 | thiI yajK | yajK | b0423 JW0413 | cytoplasmic protein, tRNA sulfurtransferase ThiI | Disulfide bridge (344 456) PMID: 16855700 | Cytoplasmic | A | A | N/A | Cytoplasm | Cytoplasmic | no | yes | N | 55 | 0.111 | CYT | 0 | -0.200913 | - | 0 | 0 | N | i | Cytoplasmic | 10 | ||||||||||
| YDES_ECOLI | P77789 | ydeS | b1504 JW1498 | Fimbrial extra-cellular peripheral OM protein (SEC through IM, chaperone usher export system over OM), YdeS | Potential fimbrial yde operon (class ??1); Fimbrial Subunit. Disulfide bridge (38 78) | Peripheral outer membrane protein facing the extra-cellular space | F4 | F4 | N/A | Fimbrium | Membrane anchored | fimbriae | SEC | CU | no | yes | Y | 23 | 0.426 | SpI | 22 | 11.5353 | - | 1 | 0 | Y | n6-17c22/23o | Extracellular | 10 | 79 | |||||||
| RL362_ECOLI | Q2EEQ2 | ykgO rpmJ2 | rpmJ2 | b4506 JW5034 | ribosomal protein, 50S L36 2 YkgO | Translation, Ribosomal. Expressed during stationary phase in minimal medium (at protein level). Disulfide bond-containing protein PMID: 25664544 | Cytoplasmic, Ribosomal | A, r | A,r | N/A | - | ribosome | no | yes | N | 38 | 0.12 | CYT | 0 | -0.200913 | - | 0 | 0 | N | i | Unknown | 2 | 136 | |||||||||
| YICS_ECOLI | Q2M7X4 | yicS | b4555 JW5965 | periplasmic protein (SEC through IM), YicS | Disulfide bond-containing protein PMID: 25664544 | Periplasmic | G | G | N/A | Periplasmic | SEC | yes | yes | N | 22 | 0.681 | SpI | 21 | 10.9898 | - | 0 | 0 | Y | n4-14c21/22o | Unknown | 2 | |||||||||||
| COPA_ECOLI | Q59385 | copA atcU f834 ybaR | atcU f834 ybaR | b0484 JW0473 | integral IM protein (SEC-YidC in IM), Copper-exporting P-type ATPase CopA | Integral Inner Membrane (Experimental) | Exhibites aberrant localization by microscopy in the absence of YidC. PMID: 10639134 Disulfide bond-containing protein PMID: 25664544 | PMID: 10639134 PMID: 15919996 | in/in | Integral Inner Membrane | B | B | IM (predicted), Detected in IM Fraction | Copper-exporting P-type ATPase: Cell inner membrane | Integral Membrane Protein (Experimental) | SRP | SEC-YidC | no | yes | N | 50 | 0.162 | CYT | 0 | -0.200913 | - | 8 | 8 | N | i185-205o217-238i245-264o284-302i436-458o464-487i778-797o803-824i | CytoplasmicMembrane | 10 | 21 |